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3rd European conference on Xylella fastidiosa and XF-ACTORS final meeting Building knowledge, protecting plant health 26-30 April 2021 Book of Abstracts draft Table of contents XF-ACTORS Final Meeting ..................................................................................................... 2 DAY 1 - Monday 26 APRIL 2021 ............................................................................................. 2 SESSION 1: Achieving practical outcomes from genetic and biological studies ..................... 2 DAY 2 – Tuesday 27 APRIL 2021 ......................................................................................... 13 SESSION 2: Vectors and control strategies ..................................................................... 13 DAY 2 – Tuesday 27 APRIL 2021 ......................................................................................... 22 SESSION 3: Control of the bacterium in the host plants .................................................. 22 DAY 3 – Wednesday 28 APRIL 2021 ..................................................................................... 27 SESSION 4: Implementation and harmonization of diagnostic protocols, surveillance and modelling .................................................................................................................... 27 3rd European conference on Xylella fastidiosa ........................................................................ 38 DAY 4 – Thursday 29 APRIL 2021 ........................................................................................ 38 SESSION 1: Advances on pathogen biology, ecology and genetics ................................... 38 SESSION 2: Understanding vectors biology and pathogen transmission ............................ 48 DAY 5 – Friday 30 APRIL 2021 .................................................................................... 57 SESSION 3: From epidemiological modelling to surveillance and early detection ................ 57 SESSION 4: Towards sustainable management strategies ............................................... 67 E-poster session ................................................................................................................. 75 Pathogen biology, ecology and genetics ....................................................................... 76 Host plant-interactions and search for resistant/tolerant germplasm ............................... 88 Epidemiology and modeling of Xylella fastidiosa diseases .............................................. 99 Vectors biology and control ....................................................................................... 105 Surveillance and early detection tools ........................................................................ 140 Endophytic microbial resources and their potential applications for Xylella fastidiosa control .............................................................................................................................. 163 Social sciences and communication ............................................................................. 176 Acknowledgments ............................................................................................................ 180 1 | XF-ACTORS Final Meeting DAY 1 - Monday 26 APRIL 2021 - 14.30-19.20 14.30-15.00 Opening ceremony and Introduction to the event SESSION 1: Achieving practical outcomes from genetic and biological studies Chairs: Donato Boscia (CNR-IPSP) and Marie-Agnès Jacques (INRAe) 15.00-15.15 Introduction and adaptation of an emerging pathogen to olive trees in Italy Anne Sicard, UC Berkeley (US) and PHIM Plant Health Institute, Montpellier (FR) 15.15-15.30 Probable dates and scenario of introduction of Xylella fastidiosa subsp. multiplex in France Enora Dupas, INRAe, Beaucouzé (FR) 15.30-15.40 Q&A 15.40-15.55 Extreme temperature differentially affects growth and survival of Xylella fastidiosa strains Miguel Román-Écija, Instituto de Agricultura Sostenible (IAS), Consejo Superior de Investigaciones Científicas (CSIC), Córdoba (ES) 15.55-16.10 Investigation of the metabolic network of Xylella fastidiosa responsible of its fastidious growth Caroline Baroukh, LIPME Université de Toulouse INRAE CNRS, Castanet-Tolosan (ES) 16.10-16.20 Q&A 16.20-16.35 Unravelling distinctive features of Xylella fastidiosa strain ‘De Donno’ Giusy D’Attoma, Istituto per la Protezione Sostenibile delle Piante, CNR, Bari (IT) 16.35-16.50 Studies on Diffusible Signal Factors molecules in Xylella fastidiosa strain De Donno Danilo Vona, Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro, Bari (IT) 16.50-17.00 Q&A 17.00-17.30 Coffee Break 17.30-17.45 Artificial inoculation of Xylella fastidiosa subsp. pauca strains in olive plants; an overview of greenhouse experiments Helvecio Della Coletta-Filho, Centro APTA Citros Sylvio Moreira, Instituto Agronômico, Cordeirópolis (BR) 17.45-18.00 Xylella fastidiosa subsp. pauca and multiplex elicit differential responses in a susceptible olive cultivar Annalisa Giampetruzzi, Istituto per la Protezione Sostenibile delle Piante, CNR, Bari (IT) 18.00-18.10 Q&A 18.10-18.25 Estimating prevalence and population size of Xylella fastidiosa in olive cultivars with differential phenotypic responses to the bacterial infection Maria Saponari, Institute for Sustainable Plant Protection CNR, Bari (IT) 18.25-18.40 Xylella fastidiosa and olive interactions: the key role of the plant cell wall Pasquale Saldarelli, Institute for Sustainable Plant Protection CNR, Bari (IT) 18.40-18.50 Q&A 18.50-19.20 Conclusions: Marie-Agnès Jacques, Donato Boscia and Pasquale Saldarelli 2 | Introduction and adaptation of an emerging pathogen to olive trees in Italy Sicard A1,2, Saponari M3, Vanhove M1, Castillo A I1, Giampetruzzi A3, Loconsole G3, Saldarelli P3, D3, Neema C2, Almeida RPP1 1 University of California, Berkeley, 94720 CA Berkeley, U.S.A. 2 Plant Health Institute of Montpellier (PHIM), 34398 Montpellier, France 3 National Research Council (CNR), Institute for Sustainable Plant Protection (IPSP), 70126 Bari, Italy The invasive plant pathogen Xylella fastidiosa currently threatens European flora through the loss of economically and culturally important host plants. Previously absent from Europe, and considered a quarantine pathogen, X. fastidiosa was first detected in Apulia, Italy in 2013 associated with a devastating disease of olive trees. Although the biology of X. fastidiosa has been studied for over a century, there is still no information on the determinants of specificity between bacterial genotypes and host plant species, which is particularly relevant today as X. fastidiosa is expanding in the naïve European landscape. We analyzed the genomes of 79 X. fastidiosa samples from diseased olive trees across the affected area in Italy as well as genomes of the most genetically closely related strains from Central America. We provided insights into the ecological and evolutionary emergence of this pathogen in Italy. We showed the pathogen was recently introduced, and we generated a list of candidate genes that could play a major role in the adaptation of X. fastidiosa to new environments. Keywords: population genomics, adaptation, Olive Quick Decline Syndrome 3 | Probable dates and scenario of introduction of Xylella fastidiosa subsp. multiplex in France Dupas E1, Durand K1, Rieux A2, Briand M1, Pruvos O2, Denancé N3, Legendre B4, Cunty A4, Cesbron S1, Ravigné V2, Jacques M A1 1 Research Institute of Horticulture and Seeds (IRHS), INRAE AGROCAMPUS-Ouest Université d'Angers, 49071 Beaucouzé, France 2 French Agricultural Research Centre for International Development (CIRAD), 97410 Saint-Pierre, La Réunion, France 3 French Variety and Seed Study and Control Group (GEVES), 49071 Beaucouzé, France 4 French Agency for Food, Environmental and Occupational Health & Safety (ANSES), 49044 Angers, France Xylella fastidiosa was detected for the first time in the French territory in 2015, in Corsica and PACA regions. Most samples were infected by strains from two lineages (ST6 and ST7) of X. fastidiosa subsp. multiplex. While numerous of foci were detected all over Corsica, in natural, urban and semi-urban environments, in a large range of hosts (39 plant species), a more limited number of mostly urban foci was reported from PACA in a range of 25 plant species, from which 15 were not reported infected in Corsica. Hence, situations look like having different histories. Our aim was to decipher the most probable scenario of introduction of each ST in these two regions. A collection of 82 genome sequences including 56 genome sequences of French strains was used to date the divergence of French X. fastidiosa subsp. multiplex strains from their American relatives. We developed a 13-VNTR scheme for direct typing of X. fastidiosa subsp. multiplex in infected plant samples and used Approximated Bayesian Computation analyses to select the best scenario to explain the spread of the bacterium in France. The scenario choice and testing will be presented together with parameters allowing the selection of the best scenario and its interpretation in the context of plant trade. 4 | Extreme temperature differentially affects growth and survival of Xylella fastidiosa strains Román-Écija M , Landa BB, Testi L, Navas-Cortés JA Instituto de Agricultura Sostenible (IAS), Consejo Superior de Investigaciones Científicas (CSIC), 14004 Córdoba, Spain The development of regionalised risk models for the establishment of Xylella fastidiosa (Xf) and its subspecies requires
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  • The Effect of Canopy Area on Ripening and Wine Quality (2017) Rosemont Vineyards and Winery Submitted by Justin Rose

    The Effect of Canopy Area on Ripening and Wine Quality (2017) Rosemont Vineyards and Winery Submitted by Justin Rose

    The Effect of Canopy Area on Ripening and Wine Quality (2017) Rosemont Vineyards and Winery Submitted by Justin Rose Summary This study examines the impact of canopy height and ripening on wine quality in Merlot. Three sets of five rows of Merlot were hedged to different heights in mid-June: 52 inches (High canopy), 44 Inches (Medium canopy, normal height), and 36 inches (Short canopy). All other vineyard treatments were identical. Not much additional shoot growth occurred after hedging. Grapes were harvested on August 25 and processed into separate T Bins. All other treatments were identical. Juice Brix was slightly higher for the short canopy compared to the higher canopy. This may have been due to a seeming resistance to rain dilution seen in the short canopy vine compared to the medium and higher canopy vines. The ethanol, TA, color, and tannin increased with decrease in canopy height, and pH decreased with canopy height. Overall, descriptive analysis had difficulty distinguishing the wines consistently. The short canopy treatment tended to have slightly more Bitterness and Overall Aromatic Intensity. The short canopy wine also exhibited some slight reduction relative to the other two wines, which may have influenced results. Fruit Intensity and Astringency tended to vary between wines between tastings. In general, the high canopy wine tended to be the most preferred. Future studies should examine how bud fruitfulness and yield are impacted by multiple vintages of heavy hedging, pick fruit at different times depending on which treatment is deemed “optimally” ripe, and hedging shoots when they reach their designated height to try to force lateral growth.