Version: 2 Revision Date: 2015-12-04

Product Data Sheet Purified anti-Hoxb-1

Catalog # / Size: 908101 / 100 µl Previously: Covance Catalog# PRB-231P Clone: Poly19081 Isotype: Rabbit Polyclonal Immunogen: The Hoxb-1 antibody was raised against a fusion containing the majority of the first exon from mouse Hoxb-1. Reactivity: Mouse, Primate Preparation: The antibody was purified by affinity chromatography. Formulation: Phosphate-buffered solution + 0.03% Thimerosal. Concentration: 1 mg/ml Storage: The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody. Applications: Applications: WB, IHC Recommended Usage: The optimal working dilution should be determined for each specific assay condition. • WB: 1:1,000* • IHC: 1:200 Application Notes: This antibody is effective in immunoblotting and immunohistochemistry (IHC). It has been used successfully in paraffin embedded tissues. *Predicted MW=32 kD This antibody was subsequently purified using protein-A chromatography and can be used to study the development and control of transcription. Application References: 1. Arenkiel BR, Tvrdik P, Gaufo GO, Capecchi MR. Hoxb1 functions in both motoneurons and in tissues of the periphery to establish and maintain the proper neuronal circuitry. Dev 18(13):1539-52, 2004. 2. Gaufo GO, Flodby P, Capecchi MR. Hoxb1 controls effectors of sonic hedgehog and Mash1 signaling pathways. Development 127(24):5343-5354, 2000. 3. Scott MP, Tamkun JW, Hartzell GW. The structure and function of the homeodomain. Biochim Biophys Acta 989 :25-48, 1989. 4. Goddard JM, Rossel M, Manley NR, Capecchi MR. Mice with targeted disruption of Hox b1 fail to form the motor nucleus of the VIIth nerve. Development 122:3217-3228, 1996. 5. Gehring WJ. in the study of development. Science 236:1245, 1987.

Description: HOXB1 ( protein B1) is a member of the homeobox family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD. HOXB1 is expressed in rhombomere-4 (r-4) of the hindbrain, where it drives the development of r-4 derived structures including the motor neurons of the facial nerve, and neural crest cells that form Schwann cells plus cartilage and bone of the neck and ear. Other Names: Homeobox protein Hox-B1, homeo box 2I, homeo box B1, homeobox protein Hox-2I Related Products:Product Clone Application Purified anti-FOX3 (NeuN) 1B7 IHC, WB, ICC Anti-HOX C8 C952-12E IHC, WB, IF Anti-Prox1 Poly19252 IHC, IF, WB Purified anti-Pax-2 Poly19010 IHC, WB, IP Purified anti-Pax-6 Poly19013 WB, IHC, IF Purified anti-Tubulin β 3 (TUBB3) TUJ1 IHC, WB, IP, ICC

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