Product Datasheet

MYOT antibody

Catalog No: #38917

Orders: [email protected] Description Support: [email protected]

Product Name MYOT antibody

Host Species Rabbit

Clonality Polyclonal

Purification Antibodies were purified by affinity purification using immunogen.

Applications WB,IHC,IF

Species Reactivity Human,Mouse,Rat

Specificity The antibody detects endogenous level of total MYOT antibody.

Immunogen Type Recombinant Protein

Immunogen Description Recombinant protein of human MYOT.

Target Name MYOT

Other Names MFM3; TTID; TTOD; LGMD1; LGMD1A;

Accession No. Swiss-Prot#: Q9UBF9NCBI Gene ID: 9499

SDS-PAGE MW 55kd

Concentration 1.0mg/ml

Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02%

sodium azide and 50% glycerol.

Storage Store at -20°C

Application Details

WB 1:500 - 1:2000IHC 1:50 - 1:200IF 1:10 - 1:100

Images

Western blot analysis of extracts of various cell lines, using MYOT at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 1 Immunohistochemistry of paraffin-embedded mouse heart using MYOT at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using MYOT . Blue: DAPI for nuclear staining.

Background

This gene encodes a cystoskeletal protein which plays a significant role in the stability of thin filaments during muscle contraction. This protein binds F-actin, crosslinks actin filaments, and prevents latrunculin A-induced filament disassembly. Mutations in this gene have been associated with limb-girdle muscular dystrophy and myofibrillar myopathies. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Note: This product is for in vitro research use only and is not intended for use in humans or animals.

Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 2