Journal of Chromatography a Flavylium Chromophores As Species Markers for Dragon's Blood Resins from Dracaena and Daemonorops
Journal of Chromatography A, 1209 (2008) 153–161 Contents lists available at ScienceDirect Journal of Chromatography A journal homepage: www.elsevier.com/locate/chroma Flavylium chromophores as species markers for dragon’s blood resins from Dracaena and Daemonorops trees Micaela M. Sousa a,b , Maria J. Melo a,b,∗ , A. Jorge Parola b , J. Sérgio Seixas de Melo c , Fernando Catarino d , Fernando Pina b, Frances E.M. Cook e, Monique S.J. Simmonds e, João A. Lopes f a Department of Conservation and Restoration, Faculty of Sciences and Technology, New University of Lisbon, 2829-516 Monte da Caparica, Portugal b REQUIMTE, CQFB, Chemistry Department, Faculty of Sciences and Technology, New University of Lisbon, 2829-516 Monte da Caparica, Portugal c Department of Chemistry, University of Coimbra, P3004-535 Coimbra, Portugal d Botanical Garden, University of Lisbon, Lisbon, Portugal e Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3AB, UK f REQUIMTE, Servic¸ o de Química-Física, Faculdade de Farmácia, Universidade do Porto, Rua Aníbal Cunha 164, 4099-030 Porto, Portugal article info abstract Article history: A simple and rapid liquid chromatographic method with diode-array UV–vis spectrophotometric detec- Received 20 May 2008 tion has been developed for the authentication of dragon’s blood resins from Dracaena and Daemonorops Received in revised form 28 August 2008 trees. Using this method it was discovered that the flavylium chromophores, which contribute to the red Accepted 3 September 2008 colour of these resins, differ among the species and could be used as markers to differentiate among Available online 7 September 2008 species. A study of parameters, such as time of extraction, proportion of MeOH and pH, was undertaken to optimise the extraction of the flavyliums.
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