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Gradients in richness and turnover of a forest passerine’s diet prior to breeding
Citation for published version: Shutt, J, Nicholls, J, Trivedi, U, Burgess, M, Stone, G, Hadfield, J & Phillimore, A 2020, 'Gradients in richness and turnover of a forest passerine’s diet prior to breeding: A mixed model approach applied to faecal metabarcoding data ', Molecular Ecology, vol. 29, no. 6. https://doi.org/10.1111/mec.15394
Digital Object Identifier (DOI): 10.1111/mec.15394
Link: Link to publication record in Edinburgh Research Explorer
Document Version: Peer reviewed version
Published In: Molecular Ecology
Publisher Rights Statement: This is the peer reviewed version of the following article: Shutt, JD, Nicholls, JA, Trivedi, UH, et al. Gradients in richness and turnover of a forest passerine's diet prior to breeding: A mixed model approach applied to faecal metabarcoding data. Mol Ecol. 2020; 29: 1199– 1213. https://doi.org/10.1111/mec.15394, which has been published in final form at https://doi.org/10.1111/mec.15394. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
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Download date: 05. Oct. 2021 1 Title: Gradients in richness and turnover of a forest passerine’s diet prior to
2 breeding: a mixed model approach applied to faecal metabarcoding data
3
4 Running title: Pre-breeding dietary gradients of a passerine
5
6 Authors: Jack D Shutt1‡, James A Nicholls1,2‡, Urmi H Trivedi3, Malcolm D Burgess4,5,
7 Graham N Stone1, Jarrod D Hadfield1#, Albert B Phillimore1#*
8
9 Affiliations:
10 1 Institute of Evolutionary Biology, The University of Edinburgh, The King’s Buildings,
11 Edinburgh, EH9 3FL, UK;
12 2 Current address: Australian National Insect Collection, CSIRO, Clunies Ross Street,
13 Acton, ACT 2601, Australia;
14 3 Edinburgh Genomics, The University of Edinburgh, The King’s Buildings, Edinburgh,
15 EH9 3FL, UK;
16 4 Centre for Research in Animal Behaviour, University of Exeter, Exeter, EX4 4QG, UK;
17 5 RSPB Centre for Conservation Science, The Lodge, Sandy, Bedfordshire, SG19 2DL, UK
18
19 ‡ and # Contributed equally.
20 Correspondence: Albert Phillimore [email protected]
21 Orcid ID’s: JDS 0000-0002-4146-8748, MDB 0000-0003-1288-1231, ABP 0000-0002-
22 6553-1553
23
1 24 Abstract
25 Little is known about the dietary richness and variation of generalist insectivorous
26 species, including birds, due primarily to difficulties in prey identification. Using
27 faecal metabarcoding we provide the most comprehensive analysis of a passerine’s
28 diet to date, identifying the relative magnitudes of biogeographic, habitat and
29 temporal trends in the richness and turnover in diet of Cyanistes caeruleus (blue tit)
30 along a 39-site, 2° latitudinal transect in Scotland. Faecal samples were collected in
31 2014-15 from adult birds roosting in nestboxes prior to nest building. DNA was
32 extracted from 793 samples and we amplified COI and 16S minibarcodes. We
33 identified 432 molecular operational taxo