Staining Consistently Clear Results Staining
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AEDIT Text Editor Iii Notational Conventions This Manual Uses the Following Conventions: • Computer Input and Output Appear in This Font
Quick Contents Chapter 1. Introduction and Tutorial Chapter 2. The Editor Basics Chapter 3. Editing Commands Chapter 4. AEDIT Invocation Chapter 5. Macro Commands Chapter 6. AEDIT Variables Chapter 7. Calc Command Chapter 8. Advanced AEDIT Usage Chapter 9. Configuration Commands Appendix A. AEDIT Command Summary Appendix B. AEDIT Error Messages Appendix C. Summary of AEDIT Variables Appendix D. Configuring AEDIT for Other Terminals Appendix E. ASCII Codes Index AEDIT Text Editor iii Notational Conventions This manual uses the following conventions: • Computer input and output appear in this font. • Command names appear in this font. ✏ Note Notes indicate important information. iv Contents 1 Introduction and Tutorial AEDIT Tutorial ............................................................................................... 2 Activating the Editor ................................................................................ 2 Entering, Changing, and Deleting Text .................................................... 3 Copying Text............................................................................................ 5 Using the Other Command....................................................................... 5 Exiting the Editor ..................................................................................... 6 2 The Editor Basics Keyboard ......................................................................................................... 8 AEDIT Display Format .................................................................................. -
COMMAND LINE CHEAT SHEET Presented by TOWER — the Most Powerful Git Client for Mac
COMMAND LINE CHEAT SHEET presented by TOWER — the most powerful Git client for Mac DIRECTORIES FILES SEARCH $ pwd $ rm <file> $ find <dir> -name "<file>" Display path of current working directory Delete <file> Find all files named <file> inside <dir> (use wildcards [*] to search for parts of $ cd <directory> $ rm -r <directory> filenames, e.g. "file.*") Change directory to <directory> Delete <directory> $ grep "<text>" <file> $ cd .. $ rm -f <file> Output all occurrences of <text> inside <file> (add -i for case-insensitivity) Navigate to parent directory Force-delete <file> (add -r to force- delete a directory) $ grep -rl "<text>" <dir> $ ls Search for all files containing <text> List directory contents $ mv <file-old> <file-new> inside <dir> Rename <file-old> to <file-new> $ ls -la List detailed directory contents, including $ mv <file> <directory> NETWORK hidden files Move <file> to <directory> (possibly overwriting an existing file) $ ping <host> $ mkdir <directory> Ping <host> and display status Create new directory named <directory> $ cp <file> <directory> Copy <file> to <directory> (possibly $ whois <domain> overwriting an existing file) OUTPUT Output whois information for <domain> $ cp -r <directory1> <directory2> $ curl -O <url/to/file> $ cat <file> Download (via HTTP[S] or FTP) Copy <directory1> and its contents to <file> Output the contents of <file> <directory2> (possibly overwriting files in an existing directory) $ ssh <username>@<host> $ less <file> Establish an SSH connection to <host> Output the contents of <file> using -
Gst Gram Staining Learning Objectives the Student Will Use Aseptic Techniques in the Safe Inoculation of Various Forms of Media
GSt Gram Staining Learning Objectives The student will Use aseptic techniques in the safe inoculation of various forms of media. Follow oral and written instructions and manage time in the lab efficiently. Use the bright field light microscope to view microbes under oil immersion, make accurate observations and appropriate interpretations and store the microscope according to lab procedures. Properly prepare a bacterial smear for accurate staining and describe the chemical basis for simple staining and negative staining. Background/Theory Differential staining distinguishes organisms based on their interactions with multiple stains. In other words, two organisms may appear to be different colors. Differential staining techniques commonly used in clinical settings include Gram staining, acid-fast staining, endospore staining, flagella staining, and capsule staining. This link to the OpenStax Microbiology text provides more detail on these differential staining techniques. (OpenStax CNX, 2018) The Gram stain is a differential staining procedure that involves multiple steps. It was developed by Danish microbiologist Hans Christian Gram in 1884 as an effective method to distinguish between bacteria containing the two most common types of cell walls. (OpenStax CNX, 2018) One type consists of an inner plasma membrane and a thick outer layer of peptidoglycan. The other type consists of a double phospholipid Figure 1 Simplified structures of Gram negative cells (left) and Gram positive bilayer with a thin layer of cells (right) peptidoglycan between the two. The Gram Staining technique remains one of the most frequently used staining techniques. The steps of the Gram stain procedure are listed below and illustrated in Figure. (OpenStax CNX, 2018) 1. -
Clostridium Difficile (C. Diff)
Living with C. diff Learning how to control the spread of Clostridium difficile (C. diff) This can be serious, I need to do something about this now! IMPORTANT C. diff can be a serious condition. If you or someone in your family has been diagnosed with C. diff, there are steps you can take now to avoid spreading it to your family and friends. This booklet was developed to help you understand and manage C. diff. Follow the recommendations and practice good hygiene to take care of yourself. C. diff may cause physical pain and emotional stress, but keep in mind that it can be treated. For more information on your C.diff infection, please contact your healthcare provider. i CONTENTS Learning about C. diff What is Clostridium difficile (C. diff)? ........................................................ 1 There are two types of C.diff conditions .................................................... 2 What causes a C. diff infection? ............................................................... 2 Who is most at risk to get C. diff? ............................................................ 3 How do I know if I have C. diff infection? .................................................. 3 How does C. diff spread from one person to another? ............................... 4 What if I have C. diff while I am in a healthcare facility? ............................. 5 If I get C. diff, will I always have it? ........................................................... 6 Treatment How is C. diff treated? ............................................................................. 7 Prevention How can the spread of C. diff be prevented in healthcare facilities? ............ 8 How can I prevent spreading C. diff (and other germs) to others at home? .. 9 What is good hand hygiene? .................................................................... 9 What is the proper way to wash my hands? ............................................ 10 What is the proper way to clean? ......................................................... -
Eosin Staining
Science of H & E Andrew Lisowski, M.S., HTL (A.S.C.P.) 1 Hematoxylin and Eosin Staining “The desired end result of a tissue stained with hematoxylin and eosin is based upon what seems to be almost infinite factors. Pathologists have individual preferences for section thickness, intensities, and shades. The choice of which reagents to use must take into consideration: cost, method of staining, option of purchasing commercially-prepared or technician-prepared reagents, safety, administration policies, convenience, availability, quality, technical limitations, as well as personal preference.” Guidelines for Hematoxylin and Eosin Staining National Society for Histotechnology 2 Why Do We Stain? In order to deliver a medical diagnosis, tissues must be examined under a microscope. Once a tissue specimen has been processed by a histology lab and transferred onto a glass slide, it needs to be appropriately stained for microscopic evaluation. This is because unstained tissue lacks contrast: when viewed under the microscope, everything appears in uniform dull grey color. Unstained tissue H&E stained tissue 3 What Does "Staining" Do? . Contrasts different cells . Highlights particular features of interest . Illustrates different cell structures . Detects infiltrations or deposits in the tissue . Detect pathogens Superbly contrasted GI cells Placenta’s large blood H&E stain showing extensive vessels iron deposits There are different staining techniques to reveal different structures of the cell 4 What is H&E Staining? As its name suggests, H&E stain makes use of a combination of two dyes – hematoxylin and eosin. It is often termed as “routine staining” as it is the most common way of coloring otherwise transparent tissue specimen. -
Powerview Command Reference
PowerView Command Reference TRACE32 Online Help TRACE32 Directory TRACE32 Index TRACE32 Documents ...................................................................................................................... PowerView User Interface ............................................................................................................ PowerView Command Reference .............................................................................................1 History ...................................................................................................................................... 12 ABORT ...................................................................................................................................... 13 ABORT Abort driver program 13 AREA ........................................................................................................................................ 14 AREA Message windows 14 AREA.CLEAR Clear area 15 AREA.CLOSE Close output file 15 AREA.Create Create or modify message area 16 AREA.Delete Delete message area 17 AREA.List Display a detailed list off all message areas 18 AREA.OPEN Open output file 20 AREA.PIPE Redirect area to stdout 21 AREA.RESet Reset areas 21 AREA.SAVE Save AREA window contents to file 21 AREA.Select Select area 22 AREA.STDERR Redirect area to stderr 23 AREA.STDOUT Redirect area to stdout 23 AREA.view Display message area in AREA window 24 AutoSTOre .............................................................................................................................. -
Laboratory Exercises in Microbiology: Discovering the Unseen World Through Hands-On Investigation
City University of New York (CUNY) CUNY Academic Works Open Educational Resources Queensborough Community College 2016 Laboratory Exercises in Microbiology: Discovering the Unseen World Through Hands-On Investigation Joan Petersen CUNY Queensborough Community College Susan McLaughlin CUNY Queensborough Community College How does access to this work benefit ou?y Let us know! More information about this work at: https://academicworks.cuny.edu/qb_oers/16 Discover additional works at: https://academicworks.cuny.edu This work is made publicly available by the City University of New York (CUNY). Contact: [email protected] Laboratory Exercises in Microbiology: Discovering the Unseen World through Hands-On Investigation By Dr. Susan McLaughlin & Dr. Joan Petersen Queensborough Community College Laboratory Exercises in Microbiology: Discovering the Unseen World through Hands-On Investigation Table of Contents Preface………………………………………………………………………………………i Acknowledgments…………………………………………………………………………..ii Microbiology Lab Safety Instructions…………………………………………………...... iii Lab 1. Introduction to Microscopy and Diversity of Cell Types……………………......... 1 Lab 2. Introduction to Aseptic Techniques and Growth Media………………………...... 19 Lab 3. Preparation of Bacterial Smears and Introduction to Staining…………………...... 37 Lab 4. Acid fast and Endospore Staining……………………………………………......... 49 Lab 5. Metabolic Activities of Bacteria…………………………………………….…....... 59 Lab 6. Dichotomous Keys……………………………………………………………......... 77 Lab 7. The Effect of Physical Factors on Microbial Growth……………………………... 85 Lab 8. Chemical Control of Microbial Growth—Disinfectants and Antibiotics…………. 99 Lab 9. The Microbiology of Milk and Food………………………………………………. 111 Lab 10. The Eukaryotes………………………………………………………………........ 123 Lab 11. Clinical Microbiology I; Anaerobic pathogens; Vectors of Infectious Disease….. 141 Lab 12. Clinical Microbiology II—Immunology and the Biolog System………………… 153 Lab 13. Putting it all Together: Case Studies in Microbiology…………………………… 163 Appendix I. -
Francisella Tularensis 6/06 Tularemia Is a Commonly Acquired Laboratory Colony Morphology Infection; All Work on Suspect F
Francisella tularensis 6/06 Tularemia is a commonly acquired laboratory Colony Morphology infection; all work on suspect F. tularensis cultures .Aerobic, fastidious, requires cysteine for growth should be performed at minimum under BSL2 .Grows poorly on Blood Agar (BA) conditions with BSL3 practices. .Chocolate Agar (CA): tiny, grey-white, opaque A colonies, 1-2 mm ≥48hr B .Cysteine Heart Agar (CHA): greenish-blue colonies, 2-4 mm ≥48h .Colonies are butyrous and smooth Gram Stain .Tiny, 0.2–0.7 μm pleomorphic, poorly stained gram-negative coccobacilli .Mostly single cells Growth on BA (A) 48 h, (B) 72 h Biochemical/Test Reactions .Oxidase: Negative A B .Catalase: Weak positive .Urease: Negative Additional Information .Can be misidentified as: Haemophilus influenzae, Actinobacillus spp. by automated ID systems .Infective Dose: 10 colony forming units Biosafety Level 3 agent (once Francisella tularensis is . Growth on CA (A) 48 h, (B) 72 h suspected, work should only be done in a certified Class II Biosafety Cabinet) .Transmission: Inhalation, insect bite, contact with tissues or bodily fluids of infected animals .Contagious: No Acceptable Specimen Types .Tissue biopsy .Whole blood: 5-10 ml blood in EDTA, and/or Inoculated blood culture bottle Swab of lesion in transport media . Gram stain Sentinel Laboratory Rule-Out of Francisella tularensis Oxidase Little to no growth on BA >48 h Small, grey-white opaque colonies on CA after ≥48 h at 35/37ºC Positive Weak Negative Positive Catalase Tiny, pleomorphic, faintly stained, gram-negative coccobacilli (red, round, and random) Perform all additional work in a certified Class II Positive Biosafety Cabinet Weak Negative Positive *Oxidase: Negative Urease *Catalase: Weak positive *Urease: Negative *Oxidase, Catalase, and Urease: Appearances of test results are not agent-specific. -
Basics of Hematology and Patho-Histology
Basics of Hematology and Patho-histology Practical Course in Molecular Pathology Winter Term 2015 Ernst Müllner MFPL (Max F Perutz Laboratories) Department of Medical Biochemistry Medical University of Vienna [email protected] www.mfpl.ac.at/mfpl-group/group/muellner.html (Müllner homepage / research) E. coli + macrophages medicalschool.tumblr.com/post/43914024728/sem-image-of-e-coli-bacteria-and-macrophages medicalschool.tumblr.com/post/18256087351/r ed-blood-cells-erythrocytes-trapped-by-fibrin Overview on main white blood cell (WBC) types – (Wikipedia) Mature white blood cell types I White Blood cells (WBCs) are frequently also referred to as peripheral blood mononuclear cells (PBMCs). Granulocytes in general are part of the innate immune system. Names derive from staining with hematoxylin and eosin. Whereas basophils stain dark blue and eosinophils are bright red, neutrophils stain neutral to pink. Basophil granulocytes Eosinophil granulocytes Neutrophil granulocytes Least common granulocyte type About 1-6% of WBCs; component Most abundant WBC type (40- (0.01- 0.3% of WBCs. Large of innate immune system to com- 75%) and essential part of the cytoplasmic granules obscure the bat parasites and certain infec- innate immune system. A patho- nucleus under the microscope. tions; also associated with allergy gen is likely to first encounter a When unstained, the nucleus is and asthma. Following activation, neutrophil. Normally contain a nu- visible and usually has 2 lobes. eosinophils effector functions in- cleus of 2-5 lobes. Neutrophils Basophils appear in inflammatory clude production and release (de- quickly congregate at a infection reactions, particularly those granulation) of cytotoxic substan- site, attracted by cytokines from causing allergies, mainly via the ces (granule proteins, reactive activated endothelium, mast cells, vasodilator histamine (antihistami- oxygen species …) and production or macrophages. -
Pattern of Cervical Cytology Using Papanicolaou Stain: an Experience from a Tertiary Hospital
Original Article Indian Journal of Forensic Medicine and Pathology Volume 13 Number 1, January - March 2020 DOI: http://dx.doi.org/10.21088/ijfmp.0974.3383.13120.12 Pattern of Cervical Cytology using Papanicolaou Stain: An Experience from a Tertiary Hospital Rashmi Shetty1, Ankitha Hebbar2, Nagarekha Kulkarni3, C Bharath4, Pavithra P5 How to cite this article: Rashmi Shetty, Ankitha Hebbar, Nagarekha Kulkarni et al. Pattern of Cervical Cytology using Papanicolaou Stain: An Experience from a Tertiary Hospital. Indian J. Forensic Med Pathol. 2020;13(1):83–88. Abstract Introduction: Cervical cancer screening using Pap smear is the cornerstone of any cancer control program. The study aimed to know the burden of various cervical lesions which were assessed by conventional Pap smear study. Methodology: We included 500 referred symptomatic patients in the study. The history, deatiled clinical examination, per speculum examination and a vaginal examination were performed for all women. Pap smear was used to screen all women for cervical cancer. Results: Mean age of the study population was 44 years and the most common complaint was whitish discharge per vaginam (54%). Classifying patients according to the Bethesda System 2001 Guidelines, we observed 61% (n = 303) cases to be Negative for Intraepithelial Lesion or Malignancy (NILM), 36% (n = 182) as Atypical Squamous Cells (ASC), 2% (n = 10) as Atypical Endocervical Cells (AEC) and 1% (n = 05) as unsatisfactory. Of the 303 cases of NILM, non-specific inflammatory changes were seen in 63%, reactive cellular changes in 21%, atrophic changes in 10%, candidiasis in 3%, Gardnerella vaginalis in 2% and inflammation with Trichomonas in 1%. -
Rapid-Air-Dry Papanicolaou Stain in Canine and Feline Tumor Cytology: a Quantitative Comparison with the Giemsa Stain
FULL PAPER Clinical Pathology Rapid-Air-Dry Papanicolaou Stain in Canine and Feline Tumor Cytology: A Quantitative Comparison with the Giemsa Stain Mariko SAWA 1), Akira YABUKI1)*, Noriaki MIYOSHI2), Kou ARAI3) and Osamu YAMATO 1) 1)Laboratory of Veterinary Clinical Pathology, Department of Veterinary Medicine, Kagoshima University, Kagoshima 890–0065, Japan 2)Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Kagoshima University, Kagoshima 890–0065, Japan 3)Kagoshima University Veterinary Teaching Hospital, Kagoshima University, Kagoshima 890–0065, Japan (Received 4 February 2012/Accepted 24 April 2012/Published online in J-STAGE 18 May 2012) ABSTRACT. The Papanicolaou stain is a gold-standard staining method for tumor diagnosis in human cytology. However, it has not been used routinely in veterinary cytology, because of its complicated multistep procedure and requirement for wet fixation. Currently, a rapid Papanicolaou stain using air-dried smears is utilized in human cytology, but usefulness of this rapid-air-dry Papanicolaou (RAD-Pap) stain in the veterinary field has not been fully evaluated. The purpose of this study was to evaluate the usefulness of the RAD-Pap stain by using quantitative analysis. Air-dried impression smears were collected from tumor specimens and stained with RAD-Pap and Giemsa. Twelve parameters representing the criteria of malignancy were quantitated, and characteristics of the RAD-Pap were evaluated statistically. The RAD-Pap stain could be applied to all the smears, and images of nucleoli and chromatin patterns were clear and detailed. In quantitative analysis with the RAD-Pap stain, but not with the Giemsa stain, dispersion of nucleolus size and dispersion of nucleolus/nucleus ratio in malignant tumors were significantly higher than those in benign tumors. -
Medical Bacteriology
LECTURE NOTES Degree and Diploma Programs For Environmental Health Students Medical Bacteriology Abilo Tadesse, Meseret Alem University of Gondar In collaboration with the Ethiopia Public Health Training Initiative, The Carter Center, the Ethiopia Ministry of Health, and the Ethiopia Ministry of Education September 2006 Funded under USAID Cooperative Agreement No. 663-A-00-00-0358-00. Produced in collaboration with the Ethiopia Public Health Training Initiative, The Carter Center, the Ethiopia Ministry of Health, and the Ethiopia Ministry of Education. Important Guidelines for Printing and Photocopying Limited permission is granted free of charge to print or photocopy all pages of this publication for educational, not-for-profit use by health care workers, students or faculty. All copies must retain all author credits and copyright notices included in the original document. Under no circumstances is it permissible to sell or distribute on a commercial basis, or to claim authorship of, copies of material reproduced from this publication. ©2006 by Abilo Tadesse, Meseret Alem All rights reserved. Except as expressly provided above, no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or by any information storage and retrieval system, without written permission of the author or authors. This material is intended for educational use only by practicing health care workers or students and faculty in a health care field. PREFACE Text book on Medical Bacteriology for Medical Laboratory Technology students are not available as need, so this lecture note will alleviate the acute shortage of text books and reference materials on medical bacteriology.