Filmtec™ Reverse Osmosis Membranes Technical Manual
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Protocols and Tips in Protein Purification
Department of Molecular Biology & Biotechnology Protocols and tips in protein purification or How to purify protein in one day Second edition 2018 2 Contents I. Introduction 7 II. General sequence of protein purification procedures 9 Preparation of equipment and reagents 9 Preparation and use of stock solutions 10 Chromatography system 11 Preparation of chromatographic columns 13 Preparation of crude extract (cell free extract or soluble proteins fraction) 17 Pre chromatographic steps 18 Chromatographic steps 18 Sequence of operations during IEC and HIC 18 Ion exchange chromatography (IEC) 19 Hydrophobic interaction chromatography (HIC) 21 Gel filtration (SEC) 22 Affinity chromatography 24 Purification of His-tagged proteins 25 Purification of GST-tagged proteins 26 Purification of MBP-tagged proteins 26 Low affinity chromatography 26 III. “Common sense” strategy in protein purification 27 General principles and tips in “common sense” strategy 27 Algorithm for development of purification protocol for soluble over expressed protein 29 Brief scheme of purification of soluble protein 36 Timing for refined purification protocol of soluble over -expressed protein 37 DNA-binding proteins 38 IV. Protocols 41 1. Preparation of the stock solutions 41 2. Quick and effective cell disruption and preparation of the cell free extract 42 3. Protamin sulphate (PS) treatment 43 4. Analytical ammonium sulphate cut (AM cut) 43 5. Preparative ammonium sulphate cut 43 6. Precipitation of proteins by ammonium sulphate 44 7. Recovery of protein from the ammonium sulphate precipitate 44 8. Analysis of solubility of expression 45 9. Analysis of expression for low expressed His tagged protein 46 10. Bio-Rad protein assay Sveta’s easy protocol 47 11. -
Evaluation of Azeotropic Dehydration for the Preservation of Shrimp. James Edward Rutledge Louisiana State University and Agricultural & Mechanical College
Louisiana State University LSU Digital Commons LSU Historical Dissertations and Theses Graduate School 1969 Evaluation of Azeotropic Dehydration for the Preservation of Shrimp. James Edward Rutledge Louisiana State University and Agricultural & Mechanical College Follow this and additional works at: https://digitalcommons.lsu.edu/gradschool_disstheses Recommended Citation Rutledge, James Edward, "Evaluation of Azeotropic Dehydration for the Preservation of Shrimp." (1969). LSU Historical Dissertations and Theses. 1689. https://digitalcommons.lsu.edu/gradschool_disstheses/1689 This Dissertation is brought to you for free and open access by the Graduate School at LSU Digital Commons. It has been accepted for inclusion in LSU Historical Dissertations and Theses by an authorized administrator of LSU Digital Commons. For more information, please contact [email protected]. This dissertation has been 70-9089 microfilmed exactly as received RUTLEDGE, James Edward, 1941- EVALUATION OF AZEOTROPIC DEHYDRATION FOR THE PRESERVATION OF SHRIMP. The Louisiana State University and Agricultural and Mechanical College, PhJD., 1969 Food Technology University Microfilms, Inc., Ann Arbor, Michigan Evaluation of Azeotropic Dehydration for the Preservation of Shrimp A Dissertation Submitted to the Graduate Faculty of the Louisiana State University and Agricultural and Mechanical College in partial fulfillment of the requirements for the degree of Doctor of Philosophy in The Department of Food Science and Technology by James Edward Rutledge B.S., Texas A&M University, 1963 M.S., Texas A&M University, 1966 August, 1969 ACKNOWLEDGMENT The author wishes to express his sincere appreciation to his major professor, Dr, Fred H. Hoskins, for the guidance which he supplied not only in respect to this dissertation but also in regard to the author’s graduate career at Louisiana State University, Gratitude is also extended to Dr. -
Assessment of End-Of-Life Opportunities for Reverse Osmosis
Assessment of End-of-Life Opportunities for Reverse Osmosis Membranes Will Lawler A thesis in fulfilment of the requirements for the degree of Doctor of Philosophy School of Chemical Engineering Faculty of Engineering March 2015 i. Abstract Reverse osmosis (RO) membranes are now core to modern desalination processes and are widely used around the world. Based on the increasing number of desalination plants, and the finite lifespan of the membranes, the resulting number of used RO modules to be discarded is becoming a critical challenge. The overall aim of this study is to identify, develop and assess alternative end-of-life options for used RO elements and investigate the associated technical readiness, environmental impact, financial considerations and legislative challenges. The assessed end-of-life alternatives include, direct reuse of the old membranes within lower throughput systems; chemical conversion into porous, ultrafiltration (UF) like filters; direct reuse or recycling of the various module components; various energy recovery techniques, and landfill disposal. The results show that direct reuse is a promising application that can be utilised with minimal additional treatment or infrastructure; however, module storage techniques are a critical consideration, particularly as membrane drying has a significant and irreversible impact on membrane performance due to pore collapse in the polysulfone support layer. The method for chemical conversion with controlled exposure to NaOCl has been optimised, resulting in promising organic and virus removal properties, comparable to commercially available 10 – 30 kDa molecular weight cut off UF membranes; however, there was significant variation in hydraulic performance, ranging from 8 – 400 L.m-2.h-1.bar-1. -
Flash Column Chromatography Guide
8.9 - Flash Column Chromatography Guide Overview: Flash column chromatography is a quick and (usually) easy way to separate complex mixtures of compounds. We will be performing relatively large scale separations in 5.301, around 1.0 g of compound. Columns are often smaller in scale than this and some of you will experience these once you move into the research lab. Column chromatography uses the same principles discussed in the TLC Handout, but can be used on a preparative scale. We are running flash columns since we will use compressed air to push the solvent through the column. This not only helps provide better separation, but also cuts down the amount of time required to run a column. Reading: For an excellent description, see LLP pages 205 - 214. Preparing and Running a Flash Column: 1) Determine the dry, solvent-free weight of the mixture that you want to separate. 2) Determine the solvent system for the column by using TLC (see TLC Handout). You should aim for Rf values between 0.2 and 0.3. If your mixture is complicated then this may not be possible. In complex cases, you will probably have to resort to gradient elution. This simply means that you increase the polarity of the solvent running through the column (eluent) throughout the course of the purification. This technique will be described in more detail later in the handout, but for the TLC analysis you should determine which solvent systems put the different spots in the 0.2 to 0.3 Rf range. 3) Determine the method of application to the column. -
Dynamics and Stability of Resonant Rings in Galaxies B
Astronomy Reports, Vol. 44, No. 5, 2000, pp. 279–285. Translated from Astronomicheskiœ Zhurnal, Vol. 77, No. 5, 2000, pp. 323–330. Original Russian Text Copyright © 2000 by Kondrat’ev. Dynamics and Stability of Resonant Rings in Galaxies B. P. Kondrat’ev Udmurt State University, Krasnoarmeœskaya ul. 71, Izhevsk, 426034 Russia Received December 24, 1998 Abstract—We have developed a model for a spheroidal, ring-shaped galaxy. The stars move in a ring with an elliptical cross section at the 1 : 1 frequency resonance. The shape of the cross section of the equilibrium ring depends on the oblateness of the galaxy itself, so that the ellipse of the ring cross section is radially extended when the oblateness of the galaxy is small. If the oblateness of galaxy exceeds some critical value, the ellipse cross section is extended along the Ox3 axis. The shape of the ring cross section is circular for a galaxy with critical eccentricity. The stability of the ring over a wide range of perturbations is studied. A fundamental bicu- bic dispersion equation for the frequencies of small oscillations of a perturbed ring is derived. Application of the model to the ring galaxy NGC 7020 shows that its ring cross section should be approximately circular. Anal- ysis of the dispersion equation demonstrates that stellar orbits in the arm are unstable (but the instability incre- ment is small). We conclude that stars in the ring of this galaxy should drift from the 1 : 1 resonance, and the ring itself should evolve. © 2000 MAIK “Nauka/Interperiodica”. 1. INTRODUCTION appearance of various commensurability ratios. -
The Avogadro Constant to Be Equal to Exactly 6.02214X×10 23 When It Is Expressed in the Unit Mol −1
[august, 2011] redefinition of the mole and the new system of units zoltan mester It is as easy to count atomies as to resolve the propositions of a lover.. As You Like It William Shakespeare 1564-1616 Argentina, Austria-Hungary, Belgium, Brazil, Denmark, France, German Empire, Italy, Peru, Portugal, Russia, Spain, Sweden and Norway, Switzerland, Ottoman Empire, United States and Venezuela 1875 -May 20 1875, BIPM, CGPM and the CIPM was established, and a three- dimensional mechanical unit system was setup with the base units metre, kilogram, and second. -1901 Giorgi showed that it is possible to combine the mechanical units of this metre–kilogram–second system with the practical electric units to form a single coherent four-dimensional system -In 1921 Consultative Committee for Electricity (CCE, now CCEM) -by the 7th CGPM in 1927. The CCE to proposed, in 1939, the adoption of a four-dimensional system based on the metre, kilogram, second, and ampere, the MKSA system, a proposal approved by the ClPM in 1946. -In 1954, the 10th CGPM, the introduction of the ampere, the kelvin and the candela as base units -in 1960, 11th CGPM gave the name International System of Units, with the abbreviation SI. -in 1970, the 14 th CGMP introduced mole as a unit of amount of substance to the SI 1960 Dalton publishes first set of atomic weights and symbols in 1805 . John Dalton(1766-1844) Dalton publishes first set of atomic weights and symbols in 1805 . John Dalton(1766-1844) Much improved atomic weight estimates, oxygen = 100 . Jöns Jacob Berzelius (1779–1848) Further improved atomic weight estimates . -
UMAP Lidars (UMBC Measurements of Atmospheric Pollution)
UMAP Lidars (UMBC Measurements of Atmospheric Pollution) Raymond Hoff Ruben Delgado, Tim Berkoff, Jamie Compton, Kevin McCann, Patricia Sawamura, Daniel Orozco, UMBC October 5 , 2010 DISCOVR-AQ Engel-Cox, J. et.al. 2004. Atmospheric Environment. Baltimore, MD Summer 2004 100 1.6 90 Old Town TEOMPM2.5 MODIS 1.4 80 MODIS AOD July 21 ) 1.2 3 70 Mixed down July 9 smoke 1 g/m 60 High altitude µ ( smoke 50 0.8 (g ) AOD 2.5 40 0.6 PM 30 0.4 20 0.2 MODISAerosol Optical Depth 10 0 0 07/01/04 07/08/04 07/15/04 07/22/04 07/29/04 08/05/04 08/12/04 08/19/04 08/26/04 Courtesy EPA/UWisconsin Conceptually simple idea Η α Extinction Profile and PBL H 100 1.6 Smoke mixing in Hourly PM2.5 90 Daily Average PM2.5 1.4 Maryland 80 MODIS AOD Lidar OD Total Column 20-22 July 2004 Lidar OD Below Boundary Layer 1.2 ) 70 3 60 1 (ug/m 50 0.8 2.5 40 0.6 PM 30 Depth Optical 0.4 20 10 0.2 0 0 07/19/04 07/20/04 07/21/04 07/22/04 07/23/04 Before upgrading: 2008 Existing ALEX - UV N2, H2O Raman lidar - 355, 389, 407 nm ELF - 532(⊥,), 1064 elastic lidar GALION needs QA/QC site for lidars in US Have all the wavelengths that people might use routinely Ability to cross-calibrate instruments Need to add MPL and LEOSPHERE Micropulse Lidar Leosphere UMBC Monitoring of Atmospheric Pollution (UMAP) (NASA Radiation Sciences Supported Project) Weblinks to instruments http://alg.umbc.edu/UMAP Data available 355 nm Leosphere retrieval (new) New instruments BAM and TEOM (PM2.5 continuously) TSI 3683 (3λ nephelometer) CIMEL (8λ AOD, size distribution) LICEL (new detector package for ALEX) MPL Shareware Concept Lidar and radiosonde measurements were also used to evaluate nearby ACARS PBLH estimates at RFK and BWI airports. -
Water Specialist for the Oil & Gas Sector Ovivowater.Com
Water Specialist for the Oil & Gas Sector creating value in water through innovation, creativity and expertise ovivowater.com © 2013 GLV Inc. All rights reserved. Ovivo - Industrial Markets Ovivo: A market Leader As society and the global economy demand more and more from water, there is a growing requirement for ever more applications to manage clean water, to create specialist process waters, to treat wastewater, to extract energy from wastewater and to champion the reuse of water. Ovivo - creating value in water through innovation, creativity and Many of the best known, most respected and reliable expertise in clean water, process brand names in the water and wastewater industries are part of Ovivo’s heritage. The combined strengths water, wastewater treatment, waste- of our brands and talents, including Brackett Green, to-energy and water reuse markets Caird & Rayner Clark, Christ Water Technology and across 5 continents. Eimco Water Technologies create one of the most comprehensive bank of technologies in the sector, one of the most impressive list of references, and practical application knowledge. Ovivo aims to become the water partner of choice for clients in the public and private sectors and the leading source of water expertise for engineers and consultants across the globe. Get in touch with some of the best brains in the business. For further information, visit ovivowater.com Ovivo - bringing water to life Copyright © 2013 GLV Inc. All rights reserved. Water Specialist for the Oil & Gas Sector Our Role in your Industry Oil and gas are precious resources. As an Ovivo develops and implements innovative integral element of oil and gas production, water solutions to meet the challenges of treating water and management is especially important. -
Page 1 of 29 Dalton Transactions
Dalton Transactions Accepted Manuscript This is an Accepted Manuscript, which has been through the Royal Society of Chemistry peer review process and has been accepted for publication. Accepted Manuscripts are published online shortly after acceptance, before technical editing, formatting and proof reading. Using this free service, authors can make their results available to the community, in citable form, before we publish the edited article. We will replace this Accepted Manuscript with the edited and formatted Advance Article as soon as it is available. You can find more information about Accepted Manuscripts in the Information for Authors. Please note that technical editing may introduce minor changes to the text and/or graphics, which may alter content. The journal’s standard Terms & Conditions and the Ethical guidelines still apply. In no event shall the Royal Society of Chemistry be held responsible for any errors or omissions in this Accepted Manuscript or any consequences arising from the use of any information it contains. www.rsc.org/dalton Page 1 of 29 Dalton Transactions Core-level photoemission spectra of Mo 0.3 Cu 0.7 Sr 2ErCu 2Oy, a superconducting perovskite derivative. Unconventional structure/property relations a, b b b a Sourav Marik , Christine Labrugere , O.Toulemonde , Emilio Morán , M. A. Alario- Manuscript Franco a,* aDpto. Química Inorgánica, Facultad de CC.Químicas, Universidad Complutense de Madrid, 28040- Madrid (Spain) bCNRS, Université de Bordeaux, ICMCB, 87 avenue du Dr. A. Schweitzer, Pessac, F-33608, France -
ABIGAIL VALANCE ©2007 Pate-Meadows Designs, Inc
ABIGAIL VALANCE ©2007 Pate-Meadows Designs, Inc. (Rev. 4/10) • This fully lined and interlined, mock Return pleated balloon shade can be adapted to fit Face Width (excluding returns) various window widths. The finished length of the valance is approximately 25”. The finished width of the valance is based on the face width excluding the returns (see illustration at right). The pattern allows for 3 ½” returns at each side where the valance 25" returns to the wall to cover the hardware. • This valance works best when constructed out of a medium-weight decorative fabric. We like to use a contrasting fabric for the pleat insert and piping. The valance is lined and interlined and has a facing along the bottom to prevent white lining from showing to the front side. We illustrate the facing out of the face fabric; however, a contrasting decorative fabric can be used for the facing instead. • Use drapery interlining to give the valance the best body and shape. Interlining is a flannel-like cotton fabric that hangs between the face fabric and the lining. It is available in white or natural colors and can be found at most decorative fabric stores or at www.patemeadows.com. It is not the same thing as interfacing . • This pattern allows for a 3 ½” return; therefore, the valance must be installed with a 3 ½” projection from the wall (see illustration above). It will not work installed on knobs alone. We recommend using decorative knobs with post adapters which are available at www.patemeadows.com. • The ties can be omitted and the valance can be hung on a decorative rod with rings as shown at right. -
February-March 2017
FROM THE QUARTERDECK FEBRUARY / MARCH 2017 THE COURSE IS SET... The process of modernizing our Jackson Creek waterfront is underway. Two committees have been formed: one We’ve been busy. Yes, really busy. to make financing recommendations for the East Dock While the winter months for most are replacement, and a second to supervise the eventual a break in sailing activities, Board construction. Steve Quiriconi and Ted Bennett are the members and many other volunteers chairs of these two committees. At its January 2017 have been planning for the season meeting, the Board approved a budget of up to $5,000 ahead. Here is an update on what’s for engineering and permitting. Even if construction in the works: does not move forward this year, the engineering and permitting work will be useful in the future. First and foremost, I am happy to announce that in late December the Long-Range Planning Committee and the Meanwhile, dredging of Jackson Creek is underway and Flag Officers concluded the search process and hired expected to conclude in several months, depending on a new General Manager. Brian Ankrom, who resides in the weather. This much-needed maintenance is intended White Stone with his wife and children, arrived on the to increase the depth and width of the channel, providing job on January 2nd ready to dive in and become part peace of mind for our larger boat owners and eliminating of FBYC. His educational and professional background in excuses for frequent bottom bumps! Various Jackson recreation and project management bring valuable skills Creek bulkhead repairs, most notably near Fannie’s to the tasks ahead. -
Since 1978 Children's Corner Has Honored the Classic Traditions Of
BY LEZETTE THOMASON Since 1978 Children’s Corner has honored the classic traditions of children’s garment sewing with timeless designs. The four original owners began a shopClaire that designed and stitched custom garments for little girls and boys. The vision of sharing these Children’s Corner designs with other heirloom shops was realized a few months later. Through the years new patterns have immerged while older ones have been retired. Children’s Corner attempts to keep up with current fashion trends, still ever respectful of a timeless look. All patterns are sized to be age appropriate. Children’s Corner prides itself on well-fitting patterns that are drafted using the excellent teachings of Elizabeth Travis Johnson. The patterns are drafted using the same sloper for each size, insuring the same fit from one pattern to the next. The pattern pieces, such as collars and sleeves, are therefore interchangeable. Original watercolor by Lucy Poyner In 1981, the Children’s Corner pattern Claire was born. Claire featured time-honored traditions – pleats, a Peter Pan collar and tiny corded piping. Lezette designed Claire to button in the front as modern mothers wanted a little girl to dress herself. Mothers also wanted to avoid Elizabeth Travis Johnson’s dreaded placket, “opening under a back pleat”. Claire, now updated, has a longer length and loose pleats rather than pressed down pleats. Lezette includes some helpful lessons in these instructions. The first is neatly finishing a sleeve edged with corded piping without bulkiness. The second lesson is how to make a machine overcasted seam look like it has been serged.