The loss of photosynthesis pathway in a holoparasitic plant Aeginetia indica revealed by plastid genome and transcriptome sequencing Jingfang Chen School of Life Sciences, Sun Yat-sen Universitu Runxian Yu School of Life Sciences, Sun Yat-sen University Jinhong Dai School of Life Sciences, Sun Yat-sen University Ying Liu School of Life Sciences, Sun Yat-sen University Renchao Zhou (
[email protected] ) Research article Keywords: Aeginetia indica, plastid genome, transcriptome Posted Date: November 27th, 2019 DOI: https://doi.org/10.21203/rs.2.17795/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Version of Record: A version of this preprint was published at BMC Plant Biology on May 8th, 2020. See the published version at https://doi.org/10.1186/s12870-020-02415-2. Page 1/16 Abstract Background With three origins of holoparasitism, Orobanchaceae provides an ideal system to study the evolution of holoparasitic lifestyle in plants. The evolution of holoparasitism can be revealed by plastid genome degradation and the coordinated changes in the nuclear genome, since holoparasitic plants lost the capability of photosynthesis. Among the three clades with holoparasitic plants in Orobanchaceae, only Clade VI has no available plastid genome sequences for holoparasitic plants. Results In this study, we sequenced the plastome and transcriptome of Aeginetia indica , a holoparasitic plant in Clade VI of Orobanchaceae, to study its plastome evolution and the corresponding changes in the nuclear genome as a response of the loss of photosynthetic function. Its plastome is reduced to 86,212 bp in size, and almost all photosynthesis-related genes were lost.