Differentially expressed as detected by Steroltalk microarray after 24h; na – not assigned

Atorvastatin 24h Rosuvastatin 24h Rifampicin 24h

Gene Symbol Description Log2 ratio p-value Log2 ratio p-value Log2 ratio p-value Bile acid synthesis Cytochrome P450, family 7, CYP7B1 subfamily B, polypeptide 1 1.16 0.000 0.61 0.042 0.18 0.359 Carbohydrate metabolism Glucose-6-phosphatase, G6PASE catalytic subunit 0.57 0.096 0.50 0.042 0.11 0.635 HK1 Hexokinase 1 -0.19 0.312 0.47 0.017 0.04 0.754 LDHA Lactate dehydrogenase A 0.81 0.045 0.32 0.339 0.32 0.293 Pyruvate dehydrogenase PDHB (lipoamide) beta 0.85 0.047 0.60 0.020 0.25 0.344 Phosphoenolpyruvate PEPCK carboxykinase 1 (soluble) 1.18 0.011 1.05 0.010 -0.05 0.865 Pyruvate kinase, liver and PK1 RBC 0.22 0.404 0.56 0.017 0.07 0.691 Cell signaling ADIPOR2 Adiponectin receptor 2 1.00 0.012 0.49 0.086 0.29 0.309 Glucokinase (hexokinase 4) GCKR regulator 0.40 0.177 0.55 0.027 0.25 0.205 LEP Leptin -0.01 0.979 -1.18 0.004 0.12 0.555 Cholesterol biosynthesis Cytochrome P450, family 51, subfamily A, polypeptide CYP51A1 1 1.85 0.000 0.85 0.015 0.19 0.477 24-dehydrocholesterol DHCR24 reductase 1.04 0.003 0.66 0.069 0.74 0.040 7-dehydrocholesterol DHCR7 reductase 1.41 0.000 1.72 0.000 0.23 0.226 Emopamil binding EBP (sterol ) 0.97 0.018 1.46 0.000 0.20 0.543 Farnesyl-diphosphate FDFT1 farnesyltransferase 1 1.43 0.006 1.82 0.000 0.21 0.565 Farnesyl diphosphate FDPS synthase 1.57 0.001 2.00 0.000 0.14 0.630 3-hydroxy-3-methylglutaryl- HMGCR Coenzyme A reductase 2.62 0.000 2.07 0.000 0.14 0.732 3-hydroxy-3-methylglutaryl- Coenzyme A synthase 1 HMGCS1 (soluble) 1.73 0.019 2.80 0.000 0.16 0.745 Hydroxysteroid (17-beta) HSD17B7 dehydrogenase 7 1.66 0.000 1.14 0.019 0.08 0.816 Isopentenyl-diphosphate IDI1 delta isomerase 1 2.91 0.000 3.18 0.000 0.26 0.504 Lanosterol synthase (2,3- oxidosqualene-lanosterol LSS cyclase) 1.28 0.006 1.28 0.000 0.14 0.633 NAD(P) dependent steroid NSDHL dehydrogenase-like 1.70 0.000 1.12 0.028 0.14 0.690 Sterol-C4-methyl oxidase- SC4MOL like 1.52 0.001 1.57 0.000 0.31 0.428 SQLE Squalene epoxidase 1.72 0.006 2.12 0.000 0.09 0.809 Drug metabolism Cytochrome P450, family 1, CYP1A1 subfamily A, polypeptide 1 0.95 0.017 1.15 0.007 0.71 0.034 Cytochrome P450, family 1, CYP1B1 subfamily B, polypeptide 1 0.30 0.401 0.70 0.015 0.09 0.633 Cytochrome P450, family 2, CYP2A13 subfamily A, polypeptide 13 1.30 0.039 -0.07 0.823 1.26 0.000 Cytochrome P450, family 2, CYP2A6 subfamily A, polypeptide 6 1.39 0.032 -0.22 0.508 1.23 0.001 Cytochrome P450, family 2, CYP2A7 subfamily A, polypeptide 7 1.49 0.030 -0.10 0.771 1.29 0.001 Cytochrome P450, family 2, CYP2B6 subfamily B, polypeptide 6 1.10 0.016 0.34 0.294 0.34 0.208 Cytochrome P450, family 2, CYP2C18 subfamily C, polypeptide 18 1.02 0.027 0.19 0.598 0.95 0.011 Cytochrome P450, family 2, CYP2C19 subfamily C, polypeptide 19 1.37 0.010 0.49 0.143 1.21 0.001 Cytochrome P450, family 2, CYP2C8 subfamily C, polypeptide 8 1.45 0.004 0.43 0.203 1.54 0.000 Cytochrome P450, family 2, CYP2C9 subfamily C, polypeptide 9 1.45 0.004 0.29 0.347 1.14 0.001 Cytochrome P450, family 2, CYP2E1 subfamily E, polypeptide 1 0.26 0.585 0.74 0.026 -0.40 0.237 Cytochrome P450, family 2, CYP2R1 subfamily R, polypeptide 1 0.75 0.019 0.15 0.628 0.07 0.775 Cytochrome P450, family 39, subfamily A, polypeptide CYP3A4 1 3.69 0.000 1.22 0.001 3.63 0.000 Cytochrome P450, family 3, CYP3A5 subfamily A, polypeptide 5 3.12 0.000 1.25 0.002 3.26 0.000 Cytochrome P450, family 3, CYP3A7 subfamily A, polypeptide 7 2.61 0.000 NA NA NA NA Fatty acid metabolism Cytochrome P450, family 4, CYP4F11 subfamily A, polypeptide 11 0.75 0.031 0.45 0.090 0.74 0.001 Cytochrome P450, family 4, CYP4F2 subfamily F, polypeptide 2 1.01 0.035 0.76 0.024 0.96 0.005 Cytochrome P450, family 4, CYP4F3 subfamily F, polypeptide 3 0.98 0.044 0.34 0.320 0.29 0.412 Cytochrome P450, family 4, CYP4F8 subfamily F, polypeptide 8 0.79 0.041 0.52 0.106 0.88 0.001 Lipase A, lysosomal acid, LIPA cholesterol esterase 0.99 0.017 0.86 0.029 0.33 0.375 Stearoyl-CoA desaturase SCD1 (delta-9-desaturase) 0.99 0.023 1.21 0.007 0.84 0.020 Heme metabolism Aminolevulinate, delta-, ALAS1 synthase 1 1.33 0.001 -0.07 0.869 1.77 0.000 Heme oxygenase HMOX1 (decycling) 1 0.70 0.036 0.68 0.032 0.63 0.026 Housekeeping genes RPS27A Ribosomal protein S27a 0.66 0.042 0.04 0.851 0.11 0.574 Lipid transport APOA4 Apolipoprotein A-IV 0.57 0.177 0.84 0.001 0.40 0.106 APOA5 Apolipoprotein A-V 1.13 0.004 0.74 0.002 0.46 0.051 APOAII Apolipoprotein A-II 0.77 0.024 0.74 0.003 0.06 0.810 APOC2 Apolipoprotein C-II 0.61 0.100 0.55 0.016 0.14 0.558 APOC3 Apolipoprotein C-III 1.06 0.012 1.18 0.000 0.12 0.589 Low density lipoprotein LDLR receptor 1.03 0.005 0.95 0.001 0.21 0.405 Nuclear receptor superfamily Nuclear receptor subfamily NR0B2 0, group B, member 2 0.76 0.032 0.33 0.212 0.16 0.402 NR1C2 1.43 0.018 1.27 0.003 0.11 0.686 Other acetyl-Coenzyme A ACAS2 synthetase 2 1.35 0.006 2.73 0.000 0.28 0.395 Acetyl-Coenzyme A acetyltransferase 2 (acetoacetyl Coenzyme A ACAT2 thiolase) 1.63 0.001 2.12 0.000 0.17 0.620 NOS2 Nitric oxide synthase 2 -0.02 0.946 0.56 0.012 0.13 0.371 SCP2 Sterol carrier protein 2 1.35 0.021 0.54 0.056 0.20 0.488 Serum FGB Fibrinogen beta chain 1.20 0.040 0.41 0.190 0.08 0.797 SREBF signaling pathway INSIG1 Insulin induced gene 1 1.54 0.002 2.11 0.000 0.30 0.459 Transcription regulators CAMP responsive element CREM modulator 0.56 0.042 0.53 0.180 0.18 0.530 Transporters ATP-binding cassette, sub- family C (CFTR/MRP), ABCC1 member 1 1.01 0.020 0.90 0.012 0.02 0.947 ATP-binding cassette, sub- family C (CFTR/MRP), ABCC2 member 2 1.23 0.017 0.37 0.383 0.94 0.013 Solute carrier organic anion transporter family, member SLCO1B1 1B1 0.75 0.025 0.05 0.907 0.24 0.449

Data represents log2 ratios of rosuvastatin, atorvastatin and rifampicin treated samples at 24h. Samples of 7 donors were analyzed using Limma. UniGene symbols are used as abbreviations.

Differentially expressed genes as detected by Steroltalk microarray after 48h; na – not assigned

Atorvastatin Rosuvastatin 48h 48h Rifampicin 48h Gene Log2 p- Log2 p- Log2 p- Symbol Gene Description ratio value ratio value ratio value Bile acid synthesis

CYP39A1 Cytochrome P450, family 3, subfamily A, polypeptide 3 0.51 0.090 -0.52 0.200 0.67 0.004

CYP7B1 Cytochrome P450, family 7, subfamily B, polypeptide 1 1.27 0.000 0.12 0.730 0.43 0.035

CYP8B1 Cytochrome P450, family 8, subfamily B, polypeptide 1 0.34 0.386 -0.64 0.036 -0.86 0.004 Carbohydrate metabolism HK2 Hexokinase 2 0.05 0.855 0.13 0.741 0.58 0.011

PDHB Pyruvate dehydrogenase (lipoamide) beta 1.04 0.016 -0.33 0.200 0.31 0.242

PCK1 Phosphoenolpyruvate carboxykinase 1 (soluble) 1.21 0.011 0.59 0.190 -0.10 0.742 Cell signaling ADIPOR1 Adiponectin receptor 1 0.73 0.048 -0.21 0.439 0.37 0.187 ADIPOR2 Adiponectin receptor 2 1.07 0.008 -0.19 0.493 0.25 0.382

CASP4 Caspase 4, apoptosis-related cysteine peptidase 0.60 0.116 -0.59 0.018 0.52 0.036

Nuclear factor of kappa light polypeptide gene enhancer in NFKB1 B-cells 1 (p105) 0.62 0.043 0.01 0.964 0.42 0.006

SUMO1 SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae) 1.03 0.044 -0.19 0.459 0.37 0.148 Cholesterol biosynthesis

CYP51A1 Cytochrome P450, family 51, subfamily A, polypeptide 1 2.42 0.000 0.86 0.014 0.31 0.257

DHCR24 24-dehydrocholesterol reductase 1.54 0.000 0.80 0.028 0.67 0.063

DHCR7 7-dehydrocholesterol reductase 2.01 0.000 1.67 0.000 0.17 0.375

EBP Emopamil binding protein (sterol isomerase) 1.97 0.000 1.20 0.000 0.17 0.604

FDFT1 Farnesyl-diphosphate farnesyltransferase 1 2.34 0.000 1.48 0.000 0.13 0.722

FDPS Farnesyl diphosphate synthase 2.54 0.000 1.88 0.000 0.21 0.487

HMGCR 3-hydroxy-3-methylglutaryl-Coenzyme A reductase 3.18 0.000 1.53 0.002 0.20 0.636

3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 HMGCS1 (soluble) 2.93 0.000 2.02 0.000 0.21 0.664

HSD17B7 Hydroxysteroid (17-beta) dehydrogenase 7 1.67 0.000 0.78 0.107 0.18 0.591

IDI1 Isopentenyl-diphosphate delta isomerase 1 3.31 0.000 2.08 0.000 0.25 0.523

LSS Lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) 2.18 0.000 1.19 0.000 0.18 0.544

NSDHL NAD(P) dependent steroid dehydrogenase-like 2.11 0.000 0.67 0.195 0.09 0.788 SC4MOL Sterol-C4-methyl oxidase-like 1.86 0.000 0.83 0.033 0.23 0.554 SQLE Squalene epoxidase 2.85 0.000 1.78 0.000 0.10 0.773 Circadian regulation

DBP D site of albumin promoter (albumin D-box) binding protein -0.04 0.854 -0.17 0.488 0.34 0.018

TIM Timeless homolog (Drosophila) 0.63 0.034 -0.06 0.819 0.26 0.213 Drug metabolism

CYP1A1 Cytochrome P450, family 1, subfamily A, polypeptide 1 1.15 0.005 0.41 0.394 0.68 0.042

CYP2A13 Cytochrome P450, family 2, subfamily A, polypeptide 13 1.50 0.019 -0.41 0.256 1.34 0.000

CYP2A6 Cytochrome P450, family 2, subfamily A, polypeptide 6 1.59 0.015 -0.77 0.023 1.34 0.000

CYP2A7 Cytochrome P450, family 2, subfamily A, polypeptide 7 1.65 0.018 -0.76 0.034 1.43 0.000

CYP2B6 Cytochrome P450, family 2, subfamily B, polypeptide 6 0.98 0.031 0.13 0.669 0.42 0.121

CYP2C18 Cytochrome P450, family 2, subfamily C, polypeptide 18 1.39 0.004 -0.36 0.314 1.01 0.007

CYP2C19 Cytochrome P450, family 2, subfamily C, polypeptide 19 1.78 0.001 -0.17 0.618 1.33 0.000

CYP2C8 Cytochrome P450, family 2, subfamily C, polypeptide 8 1.60 0.002 -0.42 0.218 1.57 0.000

CYP2C9 Cytochrome P450, family 2, subfamily C, polypeptide 9 1.64 0.001 -0.33 0.286 1.23 0.000

CYP2J2 Cytochrome P450, family 2, subfamily J, polypeptide 2 0.78 0.036 -0.16 0.586 0.04 0.870

CYP2R1 Cytochrome P450, family 2, subfamily R, polypeptide 1 0.94 0.005 -0.35 0.341 0.17 0.495

CYP3A4 Cytochrome P450, family 39, subfamily A, polypeptide 1 4.34 0.000 0.97 0.007 4.07 0.000

CYP3A5 Cytochrome P450, family 3, subfamily A, polypeptide 5 3.91 0.000 0.40 0.297 3.56 0.000

CYP3A7 Cytochrome P450, family 3, subfamily A, polypeptide 7 2.95 0.000 NA NA NA NA Fatty acid metabolism

ACSS2 Acyl-CoA synthetase short-chain family member 2 2.60 0.000 2.36 0.000 0.15 0.647

CYP4F11 cytochrome P450, family 4, subfamily F, polypeptide 11 1.04 0.004 -0.17 0.474 0.84 0.000

CYP4F2 cytochrome P450, family 4, subfamily F, polypeptide 2 1.15 0.017 -0.06 0.847 0.85 0.013

CYP4F3 cytochrome P450, family 4, subfamily F, polypeptide 3 1.04 0.034 -0.18 0.600 0.33 0.351

CYP4F8 cytochrome P450, family 4, subfamily F, polypeptide 8 1.17 0.004 -0.08 0.841 0.85 0.001

HMGCL 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase 0.59 0.045 -0.23 0.431 0.24 0.291

LIPA Lipase A, lysosomal acid, cholesterol esterase 1.25 0.003 0.10 0.784 0.35 0.343

SCD1 Stearoyl-CoA desaturase (delta-9-desaturase) 1.63 0.000 0.88 0.028 0.79 0.028 Heme metabolism

ALAS1 Aminolevulinate, delta-, synthase 1 1.18 0.004 0.28 0.544 1.42 0.000

HMOX1 Heme oxygenase (decycling) 1 0.59 0.075 0.09 0.761 0.70 0.014 Housekeeping genes

PPIA Peptidylprolyl isomerase A (cyclophilin A) 0.84 0.012 -0.29 0.286 0.40 0.163 RPL19 Ribosomal protein L19 0.75 0.054 -0.35 0.039 0.32 0.055 RPS27A Ribosomal protein S27a 0.64 0.046 0.00 0.983 0.34 0.095 Lipid transport APOA1 Apolipoprotein A-I 1.20 0.012 0.07 0.739 0.19 0.408 APOA4 Apolipoprotein A-IV 1.48 0.001 0.60 0.018 0.52 0.037 APOA5 Apolipoprotein A-V 1.20 0.003 0.08 0.714 0.22 0.354 APOAII Apolipoprotein A-II 1.64 0.000 0.22 0.342 0.22 0.363

APOB Apolipoprotein B (including Ag(x) antigen) 0.79 0.148 -0.88 0.011 0.21 0.555 APOC1 Apolipoprotein C-I 1.44 0.003 -0.18 0.446 0.59 0.017 APOC2 Apolipoprotein C-II 0.93 0.016 -0.23 0.308 0.21 0.372 APOC3 Apolipoprotein C-III 1.67 0.000 0.68 0.003 0.08 0.715

LDLR Low density lipoprotein receptor 1.52 0.000 0.91 0.001 0.30 0.246 Nuclear receptor superfamily

NR0B2 Nuclear receptor subfamily 0, group B, member 1 0.74 0.042 0.14 0.571 0.28 0.161 PPARD Peroxisome proliferator-activated receptor delta 1.90 0.005 1.19 0.005 0.17 0.527 NR1D2 nuclear receptor subfamily 1, group D, member 2 0.24 0.501 0.19 0.508 0.67 0.004 VDR vitamin D3 receptor 0.89 0.033 0.16 0.533 0.31 0.239

NR2C1 Nuclear receptor subfamily 2, group C, member 1 0.33 0.166 -0.16 0.551 0.37 0.032

NR2F2 Nuclear receptor subfamily 2, group F, member 2 0.00 0.988 -0.36 0.023 0.04 0.762

Nuclear receptor subfamily 5, group A, member 2, liver NR5A2 receptor homologus protein 1 0.00 0.985 0.00 0.985 0.40 0.003 Other

Acetyl-Coenzyme A acetyltransferase 2 (acetoacetyl ACAT2 Coenzyme A thiolase) 2.66 0.000 1.42 0.000 0.07 0.845 ORM1 Orosomucoid 1 0.22 0.515 -0.96 0.000 0.06 0.824 P53 0.30 0.302 -0.47 0.073 0.45 0.037 PON2 Paraoxonase 2 0.68 0.153 -0.74 0.008 0.46 0.104

SCARB2 Scavenger receptor class B, member 2 0.38 0.306 -0.76 0.027 0.37 0.287

UCP2 2 (mitochondrial, proton carrier) 0.59 0.018 -0.07 0.808 0.15 0.433 Serum proteins

APCS Amyloid P component, serum 0.40 0.279 -1.27 0.000 0.30 0.315 C5 Complement component 5 0.78 0.106 -0.61 0.018 0.24 0.353 C9 Complement component 9 -0.17 0.698 -0.72 0.013 -0.19 0.513

CRP C-reactive protein, pentraxin-related -0.38 0.474 -1.98 0.002 0.63 0.065

SAA2 Serum amyloid A2 (also detects SAA1) -0.24 0.595 -0.74 0.004 0.02 0.952 SAA4 Serum amyloid A4 0.54 0.096 -0.61 0.023 0.20 0.469 SREBF signaling pathway INSIG1 Insulin induced gene 1 2.24 0.000 1.86 0.000 0.24 0.559

MBTPS2 Membrane-bound transcription factor peptidase, site 2 0.09 0.813 -0.22 0.462 0.40 0.042

SREBF2 Sterol regulatory element binding transcription factor 2 0.88 0.020 0.29 0.470 0.24 0.277 Transcription regulators

CEBPA CCAAT/enhancer binding protein (C/EBP), alpha 0.45 0.131 -0.48 0.199 0.79 0.010 CREM CAMP responsive element modulator, tau 0.59 0.039 -0.15 0.723 0.31 0.285

HIF1A Hypoxia-inducible factor 1, alpha subunit 0.22 0.558 -0.27 0.460 0.88 0.007

NCOR1 Nuclear receptor co-repressor 1 0.60 0.032 -0.05 0.870 0.45 0.020 Nuclear receptor subfamily 5, group A, member 2, liver MLXIPL receptor homologus protein 1 0.85 0.044 0.14 0.637 0.33 0.268 Transporters

ABCB7 ATP-binding cassette, sub-family B (MDR/TAP), member 7 0.59 0.108 -0.43 0.197 0.53 0.046

ATP-binding cassette, sub-family C (CFTR/MRP), member ABCC2 2 1.04 0.040 0.05 0.903 1.06 0.005

ABCG2 ATP-binding cassette, sub-family G (WHITE), member 2 0.79 0.027 -0.11 0.601 0.43 0.051

ATP-binding cassette, sub-family G (WHITE), member 5 ABCG5 (sterolin 1) 0.49 0.210 -0.63 0.003 0.27 0.185

Solute carrier family 10 (sodium/bile acid SLC10A1 family), member 1 0.54 0.239 -1.17 0.007 -0.22 0.471

Solute carrier family 2 (facilitated ), SLC2A2 member 2 -0.50 0.274 -1.23 0.001 0.45 0.151

Solute carrier organic anion transporter family, member SLCO1B1 1B1 0.88 0.012 -0.32 0.517 0.76 0.021

Solute carrier organic anion transporter family, member SLCO1B1 1B2 0.32 0.227 -0.26 0.391 0.55 0.018

Data represents log2 ratios of rosuvastatin, atorvastatin and rifampicin treated samples at 48h. Samples of 7 donors were analyzed using Limma. UniGene symbols are used as abbreviations