MCP-5 (CCL12), mouse Recombinant Mouse Monocyte Chemotactic Protein-5 (CCL12)
Instruction Manual
Catalog Number D-65725 Synonyms C-C motif chemokine 12, MCP-1-related chemokine, Monocyte chemoattractant protein 5, Monocyte chemotactic protein 5, MCP-5, Small-inducible cytokine A12, Ccl12, Mcp5, Scya12 Description The MCP proteins belong to the CC chemokine family, and signal through CCR2 and, with the exception of MCP-1, other CCR receptors. The MCP proteins chemoattract and activate monocytes, activated T cells, basophils, NK cells, and immature dendritic cells. The MCP family cross- reacts across species. Recombinant murine MCP-5 is a 9.3 kDa non-glycosylated, single polypeptide chain consisting of 82 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines. The rMu MCP5 has been purified by proprietary chromatographic techniques. Quantity 20 µg Molecular Mass 9.3 kDa Source E. coli Biological-Activity Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 10.0-50.0 ng/ml. Specific Activity see below Formulation White lyophilized (freeze-dried) powder. Lyophilized from a (0.2 µm) sterile-filtered, concemtrated protein solution in 1×PBS (pH 7.4). Reconstitution Please Note: Always centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use. Purity Greater than 97% (determined by SDS-PAGE and RP-HPLC analysis). Endotoxin Level < 0.1 ng per µg (1EU/µg) of rMu MCP-5. Storage The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted MCP-5 should be used immediately or stored in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% endotoxin-free HSA or BSA; e.g. Cat.No. C-69500A). Avoid repeated freeze-thaw cycles.
05/2019