MCP-5 (CCL12), mouse Recombinant Mouse Chemotactic -5 (CCL12)

Instruction Manual

Catalog Number D-65725 Synonyms C-C motif 12, MCP-1-related chemokine, Monocyte chemoattractant protein 5, Monocyte chemotactic protein 5, MCP-5, Small-inducible A12, Ccl12, Mcp5, Scya12 Description The MCP belong to the CC chemokine family, and signal through CCR2 and, with the exception of MCP-1, other CCR receptors. The MCP proteins chemoattract and activate , activated T cells, basophils, NK cells, and immature dendritic cells. The MCP family cross- reacts across species. Recombinant murine MCP-5 is a 9.3 kDa non-glycosylated, single polypeptide chain consisting of 82 amino acid residues including the four highly conserved cysteine residues present in the CC . The rMu MCP5 has been purified by proprietary chromatographic techniques. Quantity 20 µg Molecular Mass 9.3 kDa Source E. coli Biological-Activity Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 10.0-50.0 ng/ml. Specific Activity see below Formulation White lyophilized (freeze-dried) powder. Lyophilized from a (0.2 µm) sterile-filtered, concemtrated protein solution in 1×PBS (pH 7.4). Reconstitution Please Note: Always centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use. Purity Greater than 97% (determined by SDS-PAGE and RP-HPLC analysis). Endotoxin Level < 0.1 ng per µg (1EU/µg) of rMu MCP-5. Storage The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted MCP-5 should be used immediately or stored in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% endotoxin-free HSA or BSA; e.g. Cat.No. C-69500A). Avoid repeated freeze-thaw cycles.

05/2019