佐藤 清・石渡孝行・植田雅浩・須田大樹 埼玉県立自然の博物館研究報告（Bull. Saitama Mus. Nat. Hist. [N. S.]） No. 15，25-32. March 2021 

 短  報    First record of the hard testudinarium Koch, 1844   (: ) in Saitama Prefecture, Japan   Mamoru TAKAHASHI 1)，Hitoko MISUMI 1)，Yumi BABA2)  Nobuko FUJITA3) and Hiromi FUJITA3) 4)   1) Department of Anesthesiology, Faculty of Medicine, Saitama Medical University, Moroyama-machi, Iruma-gun 350-0495, Japan  2) Graduate School, Faculty of Arts and Letters, Tohoku University, Kawauchi, Aoba-ku 980-8576, Japan  3) Mahara Institute of Medical Acarology, 56‒3 Korekuni, Aratano, Anan, Tokushima 779-1510, Japan  4) Laboratory of Microbiology, Department of Food Nutritional Sciences, University of Shizuoka, Shizuoka 422-8526, Japan   Keywords：life cycle, Sus scrofa, distribution     Introduction thrombocytopenia syndrome and spotted fever group rickettsiae  (Yu et al., 2011; Ando & Fujita, 2013; Natsuaki et al., 2014; NIID,  The hard-bodied tick Amblyomma testudinarium Koch, 1844 is 2016; Takada et al., 2019). In Japan, adult A. testudinarium  widely distributed in tropical and temperate regions in Asia, and Japan parasitizes various larger mammals, such as wild boars (Sus scrofa  is the northern limit of its habitat. (Fig. 1A) (Anastos, 1950; Teng & Linnaeus, 1758), deer (Cervus nippon Temminck, 1838), , and  Jiang, 1991; Voltzit & Keirans, 2002; Kolonin, 2009; Guglielmone et humans. In contrast, immature A. testudinarium infests mostly larger  al., 2014). In Japan, A. testudinarium is mainly found in subtropical and medium-sized mammals and reptiles, and appear to occasionally  regions, regions with warmer weather conditions, and/or temperate infests birds and amphibians. (Yamaguti et al., 1971; Kitaoka &  regions from Kanto and Hokuriku Districts to the Nansei Islands. Suzuki, 1974; Kakuda et al., 1989; Inokuma et al., 2002; Ishibashi et  However, the species was recently recorded in Tohoku District (Fig. al., 2009; Okino et al., 2012; Takahashi et al., 2012,; Takahashi et al.,   1B) (Fujita &Takada, 2019; Terada et al., 2019; Kabeya et al., 2020). 2017; Takada et al., 2019; Inoue et al., 2020).  A. testudinarium is an important vector species that transmits Herein, we describe the distribution of A. testudinarium , in  pathogens causing various human infectious diseases associated with Saitama Prefecture, Japan for the first time, and in addition, we  a high risk of fatality and/or skin problems, such as severe fever with examined the possibility of the establishment of its life cycle using

  

  

  10cm  A % & 図 4．標本写真（SMNH- Pt 6106） Fig. 1 A. Global distribution of Amblyomma testudinarium. (modified from Kolonin, 2009). B. Detailed distribution in Japan (Fujita & Takada, 2019). C. Collection sites: upper closed circle, Oyagi, Moroyama-machi; lower asterisk, Gongendo, Moroyama-machi Saitama Prefecture, Japan.

 - 25 - Mamoru TAKAHASHI・Hitoko MISUMI・Yumi BABA・Nobuko FUJITA・Hiromi FUJITA

rearing experiments under field conditions. testudinarium described by Yamaguti et al. (1971). Larva (Fig. 2 A-C）: A. Whole view. The posterior margin of Materials and Methods the scutum was approximately straight. B. Hypostome: the apex was broadly rounded, corona with 2–3 rows of fine denticles, dental Collection and identification of ticks formula 2/2. C. Identification of two spurs of Coxa l; the external spur From April 2019 to July 2020, 28 wild boars were caught during was considerably larger than the internal spur. the implementation of pest control measures in Moroyama-machi, Nymph (Fig. 2 D-F): D. Scutum and palpi. The posterior margin Ogose-machi, and Hatoyama-machi, Saitama Prefecture, Japan. of the scutum was narrowly rounded. E. Hypostome with a small Whole skin samples with hair were collected from each boar after corona of fine denticles apically. Dental formula 2/2. F. Identification death, and all ticks found on the skin were collected manually. of two long, triangular spurs of Coxa l. The external spur was larger Subsequently, the skin samples were spread, and three aluminum vats than the internal spur. (370 mm × 310 mm × 40 mm each) filled with water were placed Adult female (Fig. 3 A-D): A. Dorsal view. The posterior margin under the skin for 2–3 days. All ticks that dropped into the vats were of the scutum was broadly rounded. B. Hypostome with a large recovered and preserved in glass tubes containing 70.0% or 99.5% corona of fine denticles apically. Dental formula 5/5, the inner file of ethanol. Some ticks were placed in 10% KOH solution for 24 h. After two rows was considerably smaller. C. Coxa I with two spurs. Coxae cleaning with tap water, each tick was mounted on a glass slide with II–IV each had a single, broadly rounded spur. The spur of Coxa Ⅳ Hoyer’s solution for a detailed observation. All ticks were identified was slightly longer than those of Coxae II and III. D. Identification of under a stereomicroscope (SMZ-U ZOOM 1:10) and/or a two paddle-shaped spurs of Coxa l; the internal spur was wider, and photomicroscope (Eclipse E600 DIC), both from Nikon Corporation the external spur was larger than the internal spur. (Tokyo, Japan), using taxonomic keys with the description for Adult male (Fig. 3 E-H): E. The posterior margin of the scutum individual tick species (Yamaguti et al., 1971; Voltzit & Keirans, was narrowly rounded. F. Hypostome was similar to that of females. 2002; Fujita & Takada, 2007, 2019). In this study, only A. G. The number of spurs in each Coxa was the same as that in females, testudinarium ticks were observed. although the spur of Coxa Ⅳ was longer than that in females. H. Coxa I was the same as that in females. Observation and rearing of A. testudinarium under field  conditions Collection records of A. testudinarium Four adult females considered to be capable of laying eggs A. testudinarium was collected from three wild boars caught in owing to sufficient blood feeding and one nymph that showed two areas, that is, Gongendo (35°54′ 0″ N, 139°16′ 48″E, sufficient blood feeding were reared in glass containers (35 mm Alt. 387.8 m) and Oyagi ( 35°55′12″ N, 139°17′24″ E , diameter × 45 mm high) containing plaster of Paris mixed with Alt. 255.0 m) in Moroyama-machi, Saitama Prefecture, Japan (Fig. charcoal powder (9:1) and a small amount of water. The top of the 1C and Table 1). Case 1. On June 9, 2019, an adult female boar was container was firmly fitted with a polyethylene lid, with a thin captured in Gongendo in Moroyama-machi. One adult female and 20 polyethylene sheet underneath. Adequate humidity in the container adult male A. testudinarium were collected. The adult female and 15 was maintained by periodically adding a few drops of water. adult males infested an area near the nipples of the abdomen. Five adult males were caught moving along the side of the body (Fig. 4A). Results and Discussion All ticks were unengorged and appeared to have infested the boar shortly before capture. Identification of ticks Case 2. On May 17, 2020, an adult female boar was captured in Ticks belonging to the genus Amblyomma based on the Oyagi in Moroyama-machi. Eight A. testudinarium, including 1 adult characteristics of the palpi, scutum, eyes, and festoons were recovered female, 2 adult males, and 5 nymphs, were collected from the from the ticks collected from wild boars, and the morphological abdominal region. All adult ticks were unengorged, but the five identification of all specimens was consistent with that of A. nymphs were almost fully engorged. testudinarium by using taxonomic keys and description of A. Case 3. On May 21, 2020, an adult female boar was captured in

- 26 - - 27 - Mamoru TAKAHASHI・Hitoko MISUMI・Yumi BABA・Nobuko FUJITA・Hiromi FUJITA First record of Amblyomma testudinarium ticks in Saitama Pref.

rearing experiments under field conditions. testudinarium described by Yamaguti et al. (1971). Table 1. Collection records of Amblyomma testudinarium from Moroyama-machi, Saitama Prefecture, Japan  ） No. of A. testudinarium collected Larva (Fig. 2 A-C : A. Whole view. The posterior margin of Case No. Host species Sex Date Collection site Materials and Methods the scutum was approximately straight. B. Hypostome: the apex was Female Male Nymph Larva broadly rounded, corona with 2–3 rows of fine denticles, dental 1 Sus scrofa Adult female June 9, 2019 Gongendo 1 20 00 Collection and identification of ticks formula 2/2. C. Identification of two spurs of Coxa l; the external spur 2 Sus scrofa Adult female May 17, 2020 Oyagi 1 250 From April 2019 to July 2020, 28 wild boars were caught during was considerably larger than the internal spur. 3 Sus scrofa Adult female May 21, 2020 Oyagi 10 61 10 6 the implementation of pest control measures in Moroyama-machi, Nymph (Fig. 2 D-F): D. Scutum and palpi. The posterior margin 7RWDO 12 83 15 6 Ogose-machi, and Hatoyama-machi, Saitama Prefecture, Japan. of the scutum was narrowly rounded. E. Hypostome with a small Whole skin samples with hair were collected from each boar after corona of fine denticles apically. Dental formula 2/2. F. Identification death, and all ticks found on the skin were collected manually. of two long, triangular spurs of Coxa l. The external spur was larger Subsequently, the skin samples were spread, and three aluminum vats than the internal spur. (370 mm × 310 mm × 40 mm each) filled with water were placed Adult female (Fig. 3 A-D): A. Dorsal view. The posterior margin under the skin for 2–3 days. All ticks that dropped into the vats were of the scutum was broadly rounded. B. Hypostome with a large recovered and preserved in glass tubes containing 70.0% or 99.5% corona of fine denticles apically. Dental formula 5/5, the inner file of ethanol. Some ticks were placed in 10% KOH solution for 24 h. After two rows was considerably smaller. C. Coxa I with two spurs. Coxae cleaning with tap water, each tick was mounted on a glass slide with II–IV each had a single, broadly rounded spur. The spur of Coxa Ⅳ Hoyer’s solution for a detailed observation. All ticks were identified was slightly longer than those of Coxae II and III. D. Identification of under a stereomicroscope (SMZ-U ZOOM 1:10) and/or a two paddle-shaped spurs of Coxa l; the internal spur was wider, and photomicroscope (Eclipse E600 DIC), both from Nikon Corporation the external spur was larger than the internal spur. (Tokyo, Japan), using taxonomic keys with the description for Adult male (Fig. 3 E-H): E. The posterior margin of the scutum individual tick species (Yamaguti et al., 1971; Voltzit & Keirans, was narrowly rounded. F. Hypostome was similar to that of females. 2002; Fujita & Takada, 2007, 2019). In this study, only A. G. The number of spurs in each Coxa was the same as that in females, testudinarium ticks were observed. although the spur of Coxa Ⅳ was longer than that in females. H. Coxa I was the same as that in females. Observation and rearing of A. testudinarium under field  conditions Collection records of A. testudinarium Four adult females considered to be capable of laying eggs A. testudinarium was collected from three wild boars caught in owing to sufficient blood feeding and one nymph that showed two areas, that is, Gongendo (35°54′ 0″ N, 139°16′ 48″E, sufficient blood feeding were reared in glass containers (35 mm Alt. 387.8 m) and Oyagi ( 35°55′12″ N, 139°17′24″ E , diameter × 45 mm high) containing plaster of Paris mixed with Alt. 255.0 m) in Moroyama-machi, Saitama Prefecture, Japan (Fig. charcoal powder (9:1) and a small amount of water. The top of the 1C and Table 1). Case 1. On June 9, 2019, an adult female boar was container was firmly fitted with a polyethylene lid, with a thin captured in Gongendo in Moroyama-machi. One adult female and 20 polyethylene sheet underneath. Adequate humidity in the container adult male A. testudinarium were collected. The adult female and 15 was maintained by periodically adding a few drops of water. adult males infested an area near the nipples of the abdomen. Five adult males were caught moving along the side of the body (Fig. 4A). All ticks were unengorged and appeared to have infested the boar Results and Discussion Fig. 2. Characteristic features of larvae (A–C) and nymphs (D–F) of A. testudinarium. Abbreviations: C1–III, Coxa I–III; ca., capitulum; ch., chelicera; co., corona; h., shortly before capture. hypostome; in., internal spur; ex., external spur; sc., scutum. Identification of ticks Case 2. On May 17, 2020, an adult female boar was captured in Ticks belonging to the genus Amblyomma based on the Oyagi in Moroyama-machi. Eight A. testudinarium, including 1 adult Gongendo. In total, 87 A. testudinarium individuals, including Oviposition of adult females and developmental period of one characteristics of the palpi, scutum, eyes, and festoons were recovered female, 2 adult males, and 5 nymphs, were collected from the 10 adult females, 61 adult males, 10 nymphs, and 6 larvae, were nymph from the ticks collected from wild boars, and the morphological abdominal region. All adult ticks were unengorged, but the five collected from an area near the nipples. Among these ticks, four adult All four adult females laid eggs. During the month after capture, identification of all specimens was consistent with that of A. nymphs were almost fully engorged. females showed sufficient blood feeding and they were thought to be these four adult females (body weights of 1.712, 1.129, 0.952, and testudinarium by using taxonomic keys and description of A. Case 3. On May 21, 2020, an adult female boar was captured in capable of laying eggs (Fig. 4B). 0.701 g, respectively) laid approximately 7600, 5600, 2300, and 1900

 - 27 - Mamoru TAKAHASHI・Hitoko MISUMI・Yumi BABA・Nobuko FUJITA・Hiromi FUJITA

Fig. 3. Characteristic features of adult female (A–D) and adult male (E–H) Amblyomma testudinarium.. Abbreviations: a., genital aperture; C1–III, Coxa I–III; ca., capitulum; ch., chelicera; co., corona; e., eye; f., festoon; h., hypostome; in., internal spur; ex., external spur; sc., scutum.

Fig. 4. Amblyomma testudinarium infestation of wild boars (Sus scrofa) captured in Moroyama-machi, Saitama Prefecture, Japan. A. An adult female (a) and two males (b) that had just bitten the abdominal skin of a wild boar caught in Gongendo, Moroyama-machi on June 9, 2019. B. Five adult females (a) with sufficient blood feeding, and a male (b) that had just infested the skin near the nipple of a wild boar caught in Oyagi, Moroyama-machi on May 21, 2020.

- 28 - - 29 - Mamoru TAKAHASHI・Hitoko MISUMI・Yumi BABA・Nobuko FUJITA・Hiromi FUJITA First record of Amblyomma testudinarium ticks in Saitama Pref.

Fig. 5. Life cycle of A. testudinarium. E: egg, UL: unfed larva, EL: engorged larva, UN: unfed nymph, EN: engorged nymph, UF: unfed adult female, UM: unfed adult male, EM: engorged adult male, EF: engorged adult female.

eggs, respectively. One nymph developed into an adult female with a Regarding the criteria for the distribution of A. testudinarium, we molting period of 26 days. proposed that although this species has been collected from grazing Overall, individuals of all developmental stages except eggs cattle in Aomori Prefecture, it cannot be considered as distributed. were collected from wild boars (Table 1), and the female that showed In 2019, in Fukushima Prefecture, Tohoku District, adjacent to sufficient blood-feeding oviposited in outdoor rearing. Thus, the life Gunma Prefecture, one adult A. testudinarium was collected from an cycle of A. testudinarium was considered to be completed in Asian black bear (Ursus thibetanus) (Kabeya, et al., 2020). Fig. 3. Characteristic features of adult female (A–D) and adult male (E–H) Amblyomma testudinarium.. Abbreviations: a., genital aperture; C1–III, Coxa I–III; ca., （ capitulum; ch., chelicera; co., corona; e., eye; f., festoon; h., hypostome; in., internal spur; ex., external spur; sc., scutum. Moroyama-machi as shown in Fig. 5 modified from Takahashi, However, it is not clear whether the tick was inhabiting and breeding ） 2020 , and the tick was thought to be breeding. Therefore, further in this region. studies are necessary to elucidate the life history of this tick under Therefore, the northern distribution limit of this species in Japan natural conditions in Moroyama-machi. is currently Kanto District. It should be noted that Fujita & Takada (2019) showed that A. testudinarium is also distributed in Aomori Geographical distribution Prefecture (Fig. 1B), according to a report of Terada et al. (2019). A. testudinarium have been collected from ranging in A. testudinarium was originally a southern tick that mainly size from large to small, including birds, reptiles, and amphibians inhabited tropical and subtropical Asia, and therefore, it was not (Anastos, 1950; Yamaguti et al., 1971; Teng & Jiang, 1991; distributed in the northern part of Honshu, Japan. Takahashi et al., 2017; Takada et al., 2019). Even if they infest the According to the definition of distribution provided above, the hosts, they do not necessarily entrench and breed in that region. breeding or distribution of this species was not observed in all six Here, we propose that the original distribution of ticks depends prefectures in Kanto District, although there were fragmentary on the establishment of their life cycle and breeding of ticks in the collection records by Yamaguti et al. (1971) in Tokyo and Ibaraki area or region of collection, that is, the occurrence or lack of breeding. Prefecture by 2000. Terada et al. (2019) reported that one adult female A. In Chiba Prefecture, human infestation of this species was Fig. 4. Amblyomma testudinarium infestation of wild boars (Sus scrofa) captured in Moroyama-machi, Saitama Prefecture, Japan. A. An adult female (a) and two males testudinarium was collected from each of the grazing cattle in three reported in 2001 (Tsunoda, 2004), and since then, surveys on wild (b) that had just bitten the abdominal skin of a wild boar caught in Gongendo, Moroyama-machi on June 9, 2019. B. Five adult females (a) with sufficient blood feeding, and a male (b) that had just infested the skin near the nipple of a wild boar caught in Oyagi, Moroyama-machi on May 21, 2020. pastures in Aomori Prefecture, but it was not collected from the animals and vegetation have reported the collection and settlement of vegetation in the pastures. Therefore, the history of infestation to these this species in various areas in the southern part of the Boso Peninsula grazing cattle is unknown. in Chiba Prefecture (Takemura, et al., 2017).

 - 29 - Mamoru TAKAHASHI・Hitoko MISUMI・Yumi BABA・Nobuko FUJITA・Hiromi FUJITA

In Tochigi Prefecture, since 2015, the number of human bites by Estrada-Peña, A. & Horak, I.G., 2014. The hard ticks of the world adults and nymphs of A. testudinarium has been increasing, and this (Acari: Ixodida: Ixodidae). 738 pp., Springer, Dordrecht, has not been previously reported. Furthermore, from 2017 to 2019, Heidelberg, New York and London. there have been 62 cases of human bites by adults and nymphs of the Inokuma, H., Fujimoto, T., Hosoi, E., Tanaka, S., Fujisaki, K. & species (Shimada, et al., 2020); thus, A. testudinarium is considered to Okuda, M., 2002. Tick infestation of sika deer (Cervus nippon) in be breeding in Tochigi Prefecture. the western part of Yamaguchi Prefecture, Japan. J Vet Med Sci., In Kanagawa Prefecture, Doi et al. (2019) collected a few 64:615–617. nymphs and more than 50 larvae in tick surveys of Lake Tanzawa Inoue, Y., Natsuaki, M., and Yamanishi, K., 2020. Epidemiological and Mt. Hakone in 2018 and 2019. Therefore, A. testudinarium survey of tick bites occurring in Hyogo Prefecture from 2014 appears to have settled in this area. through 2018., Med. Entomol. Zool., 71: 31‒38. As described above, since 2000, A. testudinarium has been Ishibashi, O., Niizuma, J., Miura, A., Iizuka, S., Fujita, H., Ogura, G., reported to be distributed in the Kanto district (Chiba, Tochigi and Sakashita, M., Ganeko, H., Sunagawa, K. & Nakada, T., 2009. Kanagawa prefectures), and the distribution of this species was also Survey of parasitic ixodid ticks on small Asian mongoose on confirmed for the first time in Saitama Prefecture in the current study. Okinawajima island，Japan. Jpn. J. Zoo. Wildl. Med., 14: 51-57. The distribution seems to be expanding, however, the factors affecting (In Japanese with English abstract) this process are still unclear. Kabeya, M., Monma, N., Kumada, R., Nemoto, Y., Saito, R., Nakamura, K., Kanemitsu, K. & Fujita, H., 2020. Survey of Acknowledgments ixodid ticks parasitic on wild mammals in Fukushima Prefecture, Japan, 2016-2019. Med. Entomol. Zool., 71: 112 (In Japanese）. We thank the members of the Ogose Hunting Club, including S. Kakuda, H., Shiraishi, S. & Uchida, T., 1989. Ticks from wild Hosoya, S. Suzuki, I. Ishii, T. Ichihara, S. Kato, K. Mizumura, I. mammals in the Kyushu district including Okinawa Prefecture, Kawana, S. Yoshida, and N. Yokote, for their cooperation with tick Japan . J. Fat. Agr., Kyushu Univ., 33: 267-273. collection from wild boars. Kitaoka, S. & Suzuki, H.,1974. Reports of medico-zoological investigations in the Nansei Islands Part 2. Ticks and their References seasonal prevalences in southern Amami −oshima. Med. Entomol. Zool., 25: 21-26. (In Japanese with English abstract) Anastos, G., 1950. The scutate ticks, or Ixodidae of . Kolonin, G.V., 2009. Online. Fauna of ixodid ticks of the world. Entomol., Am., 30: 1–144. http://www.kolonin.org/ (August 24, 2009). Last accessed 8 Jun Ando, S. & Fujita, H., 2013. Diversity between spotted fever group 2010. and ticks as vector. Med. Entomol. Zool., 64: 5‒7 (In National Institute of Infectious Diseases (NIID), 2016. Severe fever Japanese with English abstract). with thrombocytopenia syndrome (SFTS) in Japan, as of Doi, K., Kato, T. & Hayama, S., 2019. Tick surveillance on the February 2016. IASR., 37: 39-40. Hakone mountain trail and in the Tanzawa Lake area. Natsuaki, M., Takada, N., Kawabata, H., Ando, S. & Yamanishi, K., Proceedings of Tanzawa-Ooyama Nature Restoration Activities 2014. Case of tick-associated rash illness caused by Amblyomma Symposium in 2019: p. 8 (In Japanese). testudinarium. J. Dermatol., 41: 834-836 Fujita H, & Takada N., 2007. Identification of immature ticks in Okino, T., Ushirogawa, H., Matoba, K. & Hatsushika, R., 2012. Japan. In: Organizing Committee of SADI, editor. Acari and Bibliographical studies on human cases of hard tick (Acarina: Emerging/Reemerging infectious diseases. Tokyo: Zenkoku Ixodidae) bites in Japan (8) Whole aspect of tick bite cases :1941 Noson Kyoiku Kyokai Publishing; pp. 53–68. (In Japanese). to 2005. Kawasaki, Med. J., 38: 143－150. (In Japanese with Fujita, H. & Takada, N., 2019. Distribution of ticks as a background English abstract). for epidemiology. 2019. In: Medical Acarology in Japan, (ed. Shimada, M., Kawabata, H., Ando, S., Hou, S., Kobayashi, Y., Hirose, Takada, N.), pp. 192-199, Hokuryukan, Tokyo (In Japanese). Y., Shuto, F., Shimizu, K., Takahashi, T. & Komatsumoto, S., Guglielmone, A.A., Robbins, R.G., Apanaskevich, D.A., Petney, T.N., 2020. Tick bites (72 cases) between 2017 and 2019 at Ashikaga

- 30 - - 31 - Mamoru TAKAHASHI・Hitoko MISUMI・Yumi BABA・Nobuko FUJITA・Hiromi FUJITA First record of Amblyomma testudinarium ticks in Saitama Pref.

In Tochigi Prefecture, since 2015, the number of human bites by Estrada-Peña, A. & Horak, I.G., 2014. The hard ticks of the world Red Cross Hospital,Tochigi Prefecture, Japan: Focus on 62 cases 39 Acari: Ixodidae. 355 pp., Science Press, Beijing. (In Chinese). adults and nymphs of A. testudinarium has been increasing, and this (Acari: Ixodida: Ixodidae). 738 pp., Springer, Dordrecht, of Amblyomma testudinarium bites. Med. Entomol. Zool., 71: Terada, Y., Takahashi, T., Abe, T. & Moriyama, Y., 2019. Three cases has not been previously reported. Furthermore, from 2017 to 2019, Heidelberg, New York and London. 219-223 (In Japanese with English abstract). of tick infestation (Amblyomma testudinarium) on grazing cattle there have been 62 cases of human bites by adults and nymphs of the Inokuma, H., Fujimoto, T., Hosoi, E., Tanaka, S., Fujisaki, K. & Takada, N., Natsuaki, M. & Fujita, H., 2019. Tick-borne infectious in Aomori Prefecture, the northern part of Honshu, Japan. Med. species (Shimada, et al., 2020); thus, A. testudinarium is considered to Okuda, M., 2002. Tick infestation of sika deer (Cervus nippon) in diseases. In: Medical Acarology in Japan, (ed. Takada, N.), pp. Entomol. Zool. 70: 167‒170. (In Japanese with English abstract). be breeding in Tochigi Prefecture. the western part of Yamaguchi Prefecture, Japan. J Vet Med Sci., 200-245, Hokuryukan, Tokyo. (In Japanese). Tsunoda, T., 2004. Tick bite cases in researchers studying deer in In Kanagawa Prefecture, Doi et al. (2019) collected a few 64:615–617. Takahashi, M., Kuriyama T. & Fujita H., 2012. Ticks (Acari: Boso Peninsula, central Japan. Med. Entomol. Zool., 55: nymphs and more than 50 larvae in tick surveys of Lake Tanzawa Inoue, Y., Natsuaki, M., and Yamanishi, K., 2020. Epidemiological Ixodidae) on terrestrial in the Izu Peninsula, Japan. Annu. 243-245. and Mt. Hakone in 2018 and 2019. Therefore, A. testudinarium survey of tick bites occurring in Hyogo Prefecture from 2014 Rep. Ohara Gen. Hosp., 52: 61–67. Voltzit, O.V. & Keirans, J.E., 2002. A review of Asian Amblyomma appears to have settled in this area. through 2018., Med. Entomol. Zool., 71: 31‒38. Takahashi, M., Misumi, H. & Tamura, H., 2017. First record in Japan species (Acari, Ixodida, Ixodidae). Acarina, 10: 95–136. As described above, since 2000, A. testudinarium has been Ishibashi, O., Niizuma, J., Miura, A., Iizuka, S., Fujita, H., Ogura, G., of tortoise tick Amblyomma geoemydae (Cantor 1847) (Acari: Yamaguti N, Tipton VJ, Keegan HL, Toshioka S., 1971. Ticks of reported to be distributed in the Kanto district (Chiba, Tochigi and Sakashita, M., Ganeko, H., Sunagawa, K. & Nakada, T., 2009. Ixodidae) parasitizing Pryer’s keelback Hebius pryeri Japan, Korea, and the Ryukyu Islands. Brigham Young Kanagawa prefectures), and the distribution of this species was also Survey of parasitic ixodid ticks on small Asian mongoose on (Boulenger, 1887) (Reptile: Colubridae). Inter. J. Acarol., 43: University Science Bulletin Biological Series, 15: i–iv, 1–226. confirmed for the first time in Saitama Prefecture in the current study. Okinawajima island，Japan. Jpn. J. Zoo. Wildl. Med., 14: 51-57. 314-319. Yu XJ, Liang MF, Zhang SY, Liu Y, Li JD, Sun YL, Zhang L, Zhang The distribution seems to be expanding, however, the factors affecting (In Japanese with English abstract) Takahashi, M. 2020. Online. Current status of Tsutsugamushi disease QF, Popov VL, Li C, Qu J, Li Q, Zhang YP, Hai R, Wu W, Wang this process are still unclear. Kabeya, M., Monma, N., Kumada, R., Nemoto, Y., Saito, R., in the Kanto district, and a topic of ticks. SADI news: Q, Zhan FX, Wang XJ, Kan B, Wang SW, Wan KL, Jing HQ, Nakamura, K., Kanemitsu, K. & Fujita, H., 2020. Survey of Information Exchange in 2020: 63-68. Organizing Committee of Lu JX, Yin WW, Zhou H, Guan XH, Liu JF, Bi ZQ, Liu GH, Acknowledgments ixodid ticks parasitic on wild mammals in Fukushima Prefecture, SADI. http://www.sadi-web-site.com/2020SADInews.pdf Ren J, Wang H, Zhao Z, Song JD, He JR, Wan T, Zhang JS, Fu Japan, 2016-2019. Med. Entomol. Zool., 71: 112 (In Japanese）. Takemura, A., Tasaki, H., Taira, M. & Fujimagari, M., 2017. XP, Sun LN, Dong XP, Feng ZJ, Yang WZ, Hong T, Zhang Y, We thank the members of the Ogose Hunting Club, including S. Kakuda, H., Shiraishi, S. & Uchida, T., 1989. Ticks from wild Geographical and monthly distribution of Amblyomma Walker DH, Wang Y, Li DX.,2011, Fever with Hosoya, S. Suzuki, I. Ishii, T. Ichihara, S. Kato, K. Mizumura, I. mammals in the Kyushu district including Okinawa Prefecture, testudinarium in Chiba Prefecture. Bull. Publ. Health Lab. Chiba thrombocytopenia associated with a novel bunyavirus in China. Kawana, S. Yoshida, and N. Yokote, for their cooperation with tick Japan . J. Fat. Agr., Kyushu Univ., 33: 267-273. Prefect., 66: 72-76. (In Japanese). N. Engl. J. Med., 364: 1523-1532 collection from wild boars. Kitaoka, S. & Suzuki, H.,1974. Reports of medico-zoological Teng, K. & Jiang, Z., 1991. Economic insect of fauna of China. Fasc. investigations in the Nansei Islands Part 2. Ticks and their

References seasonal prevalences in southern Amami −oshima. Med. Entomol. Zool., 25: 21-26. (In Japanese with English abstract) Anastos, G., 1950. The scutate ticks, or Ixodidae of Indonesia. Kolonin, G.V., 2009. Online. Fauna of ixodid ticks of the world. Entomol., Am., 30: 1–144. http://www.kolonin.org/ (August 24, 2009). Last accessed 8 Jun Ando, S. & Fujita, H., 2013. Diversity between spotted fever group 2010. rickettsia and ticks as vector. Med. Entomol. Zool., 64: 5‒7 (In National Institute of Infectious Diseases (NIID), 2016. Severe fever Japanese with English abstract). with thrombocytopenia syndrome (SFTS) in Japan, as of Doi, K., Kato, T. & Hayama, S., 2019. Tick surveillance on the February 2016. IASR., 37: 39-40. Hakone mountain trail and in the Tanzawa Lake area. Natsuaki, M., Takada, N., Kawabata, H., Ando, S. & Yamanishi, K., Proceedings of Tanzawa-Ooyama Nature Restoration Activities 2014. Case of tick-associated rash illness caused by Amblyomma Symposium in 2019: p. 8 (In Japanese). testudinarium. J. Dermatol., 41: 834-836 Fujita H, & Takada N., 2007. Identification of immature ticks in Okino, T., Ushirogawa, H., Matoba, K. & Hatsushika, R., 2012. Japan. In: Organizing Committee of SADI, editor. Acari and Bibliographical studies on human cases of hard tick (Acarina: Emerging/Reemerging infectious diseases. Tokyo: Zenkoku Ixodidae) bites in Japan (8) Whole aspect of tick bite cases :1941

Noson Kyoiku Kyokai Publishing; pp. 53–68. (In Japanese). to 2005. Kawasaki, Med. J., 38: 143－150. (In Japanese with Fujita, H. & Takada, N., 2019. Distribution of ticks as a background English abstract). for epidemiology. 2019. In: Medical Acarology in Japan, (ed. Shimada, M., Kawabata, H., Ando, S., Hou, S., Kobayashi, Y., Hirose, Takada, N.), pp. 192-199, Hokuryukan, Tokyo (In Japanese). Y., Shuto, F., Shimizu, K., Takahashi, T. & Komatsumoto, S., Guglielmone, A.A., Robbins, R.G., Apanaskevich, D.A., Petney, T.N., 2020. Tick bites (72 cases) between 2017 and 2019 at Ashikaga

 - 31 - Mamoru TAKAHASHI・Hitoko MISUMI・Yumi BABA・Nobuko FUJITA・Hiromi FUJITA

埼玉県におけるタカサゴキララマダニの初記録

高橋 守 1)・三角仁子 1)・馬場裕美 2)・藤田信子 3)・藤田博己 3) 4)

1) 埼玉医科大学医学部麻酔科 〒350-0495 埼玉県入間郡毛呂山町毛呂本郷 38 2) 東北大学大学院文学研究科 〒980-8576 宮城県仙台市青葉区川内 27 番 1 号 3) 馬原アカリ医学研究所 〒779-1510 徳島県阿南市新野町是国 56-3 4) 静岡県立大学食品栄養科学部食品生命科学科微生物学研究室 〒422-8526 静岡県静岡市駿河区谷田 52-1

キーワード生活環、イノシシ、分布

- 32 - - 33 -