Intramedullary Spinal Glioblastoma Metastasis from a Brainstem Anaplastic Astrocytoma: a Case Report and Review of the Literatur

Case Report ISSN: 2631-4339

Intramedullary spinal glioblastoma metastasis from a brainstem anaplastic astrocytoma: A case report and review of the literature Daniella T Fofe*, Juan Gonzalez, Germain Milbouw and Guus Koerts Department of Neurosurgery, Centre Hospitalier Regional, Namur, Belgium

Abstract Background: Brainstem gliomas in adults are a rare and heterogeneous group of brain tumors that vary with regard to underlying pathology, radiographic appearance, clinical course and prognosis. Diffuse intrinsic pontine gliomas represent the most common subtype. In the literature there are not many cases of spinal metastatic glioblastoma from previous intracranial high- grade glioma. We report the clinical presentation, immunohistochemistry (IHC), radiological presentation and the operative management of a cervical intramedullary spinal glioblastoma from a previous brainstem anaplastic astrocytoma, but also a brief review of the literature about this topic. Case presentation: A 25-year-old male patient underwent in 2016, a stereotactic biopsy for a brainstem lesion. Anatomopathologic analysis revealed a diffuse anaplastic astrocytoma. He then received chemotherapy with Temozolomide and radiotherapy. Clinical and radiological evolution has been quite good, but three years later, he developed a worsening of the right hemiparesis, a new onset of left hemiparesthesia and left lower limb hemi anaesthesia. A full spine MRI showed a C2-C3 nodular intramedullary lesion. A surgical resection of this lesion was performed. A glioblastoma was diagnosed. Further radio- chemotherapy was given and the follow- up showed no recurrence 6 months after. Conclusion: Intramedullary metastasis from intracranial glioma are rare but possible. Despite the progress in the management of glioblastoma, this pathology should be recognized. Clinical physicians should be aware about the significance that secondary spinal glioblastoma might ensue in patients with intracranial gliomas.

Introduction [5]. Here we report very rare case of metastatic spinal glioblastoma originated from a previous brainstem high grade glioma in a young Glial tumors represent 30% of primary tumors of the central male patient. We present the pathology, the immunohistochemistry nervous system (CNS) in adults and 81% of malignant CNS tumors and the management of the disease. diagnosed in the USA [1]. Glioblastoma multiforme (GBM) was ﬁrst described by Rudolph Virchow in 1863 and represents the most Case presentation malignant tumor of the cerebral hemispheres in adult population [2]. These tumors are associated with a very poor prognosis with actually a Medical history and examination median survival between 15-18 months [3]. GBM is usually localized in A 25-year-old male patient, without systemic disease, presented to the supratentorial parts of the brain and infratentorial localization are the department of neurosurgery at the Hospital of Namur in Belgium, rare situations [4]. Anaplastic astrocytoma also pertains to aggressive in August 2016 for right hemiparesia, right facial palsy, dysarthria and behaviour and the potential of malignant transformation into diplopia. A full cerebral magnetic resonance imaging (MRI) showed a glioblastoma, with 23% of 5‑year survival rate [5]. Brainstem tumors large pontine infiltrating tumor, with surrounding oedema compatible occur more frequently in children (10-20% of all tumors) and 90% of with a brainstem glioma (Figure 1A and 1B). them are gliomas [6]. In adults, brainstem tumors account for only 3-5% [7]. In virtue of their deep location and the presence of vital neural A stereotactic biopsy of this lesion was performed in September centers within, they are associated with a poor prognosis. The common 2016. The histopathology demonstrated an anaplastic astrocytoma causes of death are loco regional extension and / or recurrence and, less (grade III OMS Classification), with a Ki67 around 10-15%. IHC frequently, leptomeningeal dissemination [8]. Brainstem tumors are more commonly encountered in children and represent 10% of all paediatric brain tumors. They represent only *Correspondence to: Daniella Tsague Fofe, Department of Neurosurgery, 1% to 2% of all brain tumors in adults and commonly involve the pons Centre Hospitalier Regional (CHR) Avenue Albert Ier, 185, Namur, Belgium, [email protected] (60%-63% of tumors). They are also identified in the medulla oblongata (25% of tumors) and the midbrain (12%-15% of tumors) [22]. Spinal Key words: anaplastic glioma, intramedullary glioblastoma, spinal matastasis, metastatic glioblastoma, which is a very infrequent clinical condition, gfap expression is usually the result of tumoral cells dissemination from a previous Received: October 19, 2020; Accepted: October 29, 2020; Published: November intracranial glioblastoma via the cerebrospinal fluid seeding pathway 05, 2020

Neuro Neurosurg, 2020 doi: 10.15761/NNS.1000138 Volume 4: 1-5 Fofe DT (2020) Intramedullary spinal glioblastoma metastasis from a brainstem anaplastic astrocytoma: A case report and review of the literature

analysis showed positive glial fibrillar acidic protein (GFAP) (Figure 2), high Ki-67 labeling index (10 – 15%), positive tumor protein P53 (TP53) mutation and a negative finding of isocitrate dehydrogenase-1 (IDH-1). A K27M mutation in histone H3.3 was also found. He then received combined radiotherapy in total of 54 Gy distributed in 1.8 Gy/day and chemotherapy with Temozolomide (75mg/m2). This treatment resulted in a quite good clinical and radiological evolution (Figure 1C), with improvement of motor deficit. Figure 3. Preoperative cervical MRI showing an intramedullary C2-C3 lesion on T2- Despite the persistence of right facial palsy (House-Brackmann Weighted cervical MRI (A), axial contrast-enhanced T1-weighted (B) and Flair sequence (C). Note that this lesion is associated with a cranial kystic cavity and a medullar oedema [4]), the patient was satisfied with the result and started working again. Three years later, he developed a worsening of his neurological Histopathologic diagnosis and safe maximal resection of the lesion condition. He was readmitted on October 2019, owing to this new were the goals of the suggested surgery. onset of symptoms for a check-up. To facilitate tumoral removal, fluorescence-guided surgery (FGS) The neurological examination revealed a worsening of the using 5-aminolevulinic acid (5-ALA) was used. That means the patient right hemiparesis, a left-sided hemiparesthesia and left lower limb received three hours before reaching the operating room, an oral anaesthesia. solution of 5 – aminolevulinic acid (5-ALA) also known as Gliolan. Cranial and whole spinal magnetic resonance imaging (MRI) The patient was placed in prone, with the head fixed in a Mayfield examinations were performed. Cranial MRI showed at C2-C3 level, headrest, perfectly exposing the upper cervical region. The incision a nodular process measuring 36 × 10 × 9 MM, hyperintense on T2- location was defined using a fluoroscopy, guided by the occipito- weighted and on Flair sequences and heterogeneously enhanced in cervical landmarks. We realized a midline posterior approach and then periphery after injection of gadolinium (Figure 3). a laminectomy of C1, C2 and C3 was performed. On the MRI imaging, the lesion seems to be right laterally located within the spinal cord. Surgery and pathology We perform under microscope a right paramedian linear opening The surgical management of the cervical metastatic lesion of the dura-mater, allowing immediate visualization of a light red consisted in a gross total resection (GTR) under general anaesthesia. coloured lesion inside the right hemi medulla flowering under the thickened arachnoid. The arachnoid was opened and a lateral incision of the medulla just in front of the emergence of the posterior roots was done. There seemed to be a boundary between the tumor and the medulla. The tumor was progressively aspirated and removed largely using an Ultrasonic Surgical Aspirator (Figure 5). Unfortunately, the tumoral cells showed no fluorescence with Gliolan uder microscope. Nevertheless, we performed a GTR . Histopathologic examination revealed a glioblastoma (grade IV OMS Figure 1. Preoperative cervical MRI showing a diffuse glial lesion developed in the annular protuberance of the brainstem (white arrow) on sagittal T2-weighted sequence (A), axial classification). IHC studies showed a high rate of MGMT mutation T2-weighted sequence (B) and axial T2-weighted sequence 2 years after multimodal (30%), a strong expression of glial fibrillar acidic protein (GFAP) treatment of the pontine glioma (C). (Figure 4). There was no expression of isocitrate dehydrogenase 1. The patient received a further concurrent chemoradiotherapy (CCRT) following the STUPP scheme. The evolution was quite good, and the patient recovered from his right hemiparesis so that he could walk without any additional help. The follow up was done using cerebral and full spine MRI examination and the last MRI, performed 6 months after the surgery revealed no residual tumor or tumoral recurrence (Figure 6). Currently he has recovered from his neurological deficits and thus he pursues a normal life and a satisfactory professional activity. Discussion Our young patient presented a brainstem anaplastic glioma responsible of a metastatic cervical spinal glioblastoma. The relationship between this brainstem lesion and the spinal cord tumor might seem uncertain. In this case we tried to reassemble the criteria proposed by Weiss in 1955 to establish an acceptable metastatic link Figure 2. Biopsy of the brainstem high grade glioma with A and B GFAP-positive cells in IHC; C and D hematoxylin and eosin staining showing microvascular proliferation and between the primary tumor and the spinal metastatic glioblastoma. The many mitoses (40X magnification) Weiss criteria are 1. the presence of a single histological characteristic

Neuro Neurosurg, 2020 doi: 10.15761/NNS.1000138 Volume 4: 2-5 Fofe DT (2020) Intramedullary spinal glioblastoma metastasis from a brainstem anaplastic astrocytoma: A case report and review of the literature

that the upper cervical region was not within or at the margin of the field of irradiation. Better still, the same fatty changes of bone narrow are now visible in the cervical vertebrae after irradiation of the tumoral bed (Figure 6A). High grade brainstem glioma is a less frequent entity in adults than in children (<2%) (9,22) and it is not that usual to deal with intramedullary metastasis originated from this type of tumors [10]. The most common location of brainstem gliomas is known to be the pons followed by medulla and midbrain [11,22]. Glioblastoma, contrarily are rarely encountered in the brainstem. Clinically, brainstem gliomas present with symptoms like multiple cranial nerve palsy and long tract signs [12]. It was clearly the case of our patient who presented with right hemiparesia, right facial palsy, dysarthria and diplopia. Guillamo et al. [13] have classified brainstem gliomas in these groups : adult diffuse infiltrative low-grade gliomas, adult malignant brainstem glioma, focal tectal brainstem glioma and others. Otherwise, Epstein and Farmer [14] presented another classification of brainstem gliomas : they separated them into diffuse, focal, exophytic and cervico-medullary. According to these classifications, we can deduce that the primary lesion we refer to in this case report was an adult diffuse malignant brainstem glioma.

Figure 4. Histologic studies of the intramedullary lesion confirming a GBM with: A: increased cellularity and frequent mitoses and necrosis on hematoxylin and eosin staining (9 X magnification) and B: GFAP-positive cells in IHC (20 X magnification)

Figure 6. Postoperative MRI images. A: Sagittal T2-weighted sequence showing a little intramedullary cavity filled by LCR, and no residue or recurrence of the C2-C3 lesion. B: Coronal Flair sequence showing no sign of intramedullary lesion and a right-side intramedullary resection cavity (Blu arrow). C: Sagittal contrast- enhanced T1-weighted sequence showing no enhancement in the medulla Figure 5. The dura mater (DM) has been opened allowing the visualization of the medulla and the cervical roots. A: Part of the tumor have been removed. The blue arrow shows a remnant of this light red coloured tumor. B: Intramedullary cavity after total removal of the tumor tumor of the central nervous system; 2. a clinical history which demonstrate that the tumor accounted for the initial symptoms; 3. a complete necropsy to exclude any other primitive tumor ; 4. identical morphology of the primary lesion and the metastasis. In our case, the majority of the criteria were fulfilled except for the third one because the patient is alive and the check-up didn’t show any other tumoral location. The spinal glioblastoma might be consider as induced by radiotherapy. This hypothesis is invalidated because the GBM occurred out of the margin of the previously irradiated field. In fact, the planned target volume included the brainstem tumor as defined by the T2- weighted MRI images with a margin of 1cm (Figure 7A). Moreover, we can notice fatty changes in clivus bone narrow Figure 7. T2-weighted MRI showing A: the planned target volume including the brainstem tumor with a margin of more than 1 cm. The cervical region is out of irradiation field. B: (Figure 7A), corresponding to the irradiation field of brainstem tumor. The fatty changes in the occipital and clivus narrow bone, due to previous radiotherapy It’s clearly not the case of the cervical vertebrae. It is the confirmation (blue arrows)

Neuro Neurosurg, 2020 doi: 10.15761/NNS.1000138 Volume 4: 3-5 Fofe DT (2020) Intramedullary spinal glioblastoma metastasis from a brainstem anaplastic astrocytoma: A case report and review of the literature

As much as it is clear that the management of glioblastoma includes Another retrospective review on 157 patients conducted by Arita et the administration of radiotherapy and concurrent temozolomide, that al. [19] concluded in similar findings. They found less GFAP expression have been shown to improve the survival in patients; in case of high in both leptomeningeal lesion and intracranial primary tumor of all the grade brainstem glioma, radiotherapy remains the standard treatment patients who presented leptomeningeal dissemination. for adult brainstem gliomas [22]. However, in exophytic lesions, a debulking as large as possible, avoiding brainstem dysfunction and For this case review, we manage to find tissues samples of both preventing impairment of quality of life as well is indicated [22]. In spinal secondary glioblastoma and previous brainstem glioma. The order to make an accurate diagnosis, it is recommended to perform immunohistochimical analysis showed in both cases a high expression a surgical biopsy if possible, in high grade diffuse gliomas [22]. A of the GFAP. These findings support the hypothesis that highly positive impact on overall survival have been shown in patient who has differentiated astrocytic tumor like our brainstem high grade glioma, undergone a surgical procedure. In our case the location, the diffuse show more conventional infiltrative behavior and are more likely to character and infiltrative behaviour did not allow a surgical treatment. induce intramedullary metastases, contrarily to poorly differentiated Our therapeutic strategy consisted in a stereotactic biopsy followed by glioma cells which frequently disseminate via the CSF pathway. a radio-chemotherapy, with a good evolution over more than three years. The immunohistochemistry of the brainstem tissue samples revealed a K27M mutation in histome H3.3. According to the literature, non-malignant brainstem glioma has a better prognosis in adults. This statement is clearly not true for A K27M mutation in histone 3 has been described to identify malignant gliomas, which prognosis is around 11 to 17 months [12,16]. high-grade midline gliomas associated with a particularly unfavorable prognosis [15]. Spinal cord GBM represent 1-5% of all the GBM and 1-3% of all the spinal cord tumors [5]. It is important to distinguish primary GBM This case presentation underlines the significance of keeping in from secondary ones. The difference between the two cases relies on mind spinal metastasis as a delayed complication of high-grade glioma. the presence or not of a pre-existing less aggressive glioma [5]. In the In general, earlier diagnosis of dissemination can lead to improvement present case, the patient was treated for a previous high grade glioma in the brainstem and then he was diagnosed for a spinal metastatic Conclusion glioblastoma. We presented a rare case of an intramedullary C2-C3 metastatic According to the literature, 20% of patient presenting high grade glioblastoma from a previous anaplastic brainstem astrocitoma (WHO intracranial gliomas develop later leptomeningeal and intramedullary grade III) in a 25-year-old male patient. We clearly demonstrate that dissemination [10]. A malignant transformation of an anaplastic the spinal lesion was not induced by radiotherapy but a real metastasis. intracranial astrocytoma into a spinal GBM is less frequent. It is usually Despite the progress in management of high-grade gliomas, recurrences a local recurrence or a dissemination along the white matter tract [2,5]. and dissemination are not rare events. Spinal metastasis are more frequently seen in younger patients Cervical spinal cord metastasis is not usual as second localization [17] and that can be explained by the simple fact that elderly patients site. Regardless of the treatment, even if this clinical condition occurs succumb more quickly to neoplastic pathologies than younger patient, more frequently in young patients, the prognosis is generally poor who live longer even with malignant glioma. and its presence tends to signalize end-stage cancer [21]. So, it is The common ways for tumoral cells to spread are thought to be via fundamental to investigate the full spinal cord in order to find earlier the cerebrospinal fluid (CSF) pathway either from direct spread into spinal metastatic lesions, even in healthy, young patients with lower the subarachnoid space or from iatrogenic spread following surgical grade gliomas with clinical and radiological signs of remission. manipulation [18,19]. The usual locations of intraspinal metastases are lower thoracic and lumbar spine [18]. Exceptionally our patient Highlights presented a cervical metastasis. • Brainstem glioma is a very rare entity in adults (1-2%). The most common symptoms of a spinal metastasis are radicular • Spinal cord GBM represent 1-5% of all the GBM and 1-3% of all the or local pain, paresthesia’s and other sensory symptoms followed by spinal cord tumors. motor weakness [18]. Paresthesia, dysesthesia and right hemiparesis were the symptoms we observed, which motivated our investigations. • Intramedullary metastatic lesion should be highly suspected in all patients with a history of intracranial high-grade glioma who Despite the primary brainstem disease was under control this complain about local spinal symptoms and signs or symptoms not young patient developed an intramedullary metastasis. explained by the primary lesion. James Powell [18] Reported cases of intramedullary metastases in • The ways of dissemination are via the CSF pathways, from direct patients with stable intracranial disease and that’s why it’s important spread into the subarachnoid space or from iatrogenic spread to perform a regular follow up of these patients with MRI imaging, following surgical manipulation. especially when they are young. The GFAP (Glial Fibrillar Acid protein) has been found to be • GFAP expression is a one of the most important determinants of the the most reliable marker of astrocytic tumors differentiation [10]. dissemination of glial tumoral cells. According to K Onda [20], in a clinicopathological study of 14 cases Acknowledgments examined by complete autopsy, glial tumors with GFAP negative cells tend to be locally less invasive but they disseminate vigorously through We are very thankful to the pathologists of UZ Brussel Institute of CSF, whereas the GFAP positive cells gliomas tended to be invasive and anatomo-pathology and Gosselies Institute of Pathology and Genetics showed infiltrative behaviour at the primary tumoral site. (IPG) for providing us the histopathological analysis our patient.

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Copyright: ©2020 Fofe DT. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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