Petroica Boodang) with the Pacific Robin (Petroica Multicolor) Which Is Found on Nearby Islands, Such As Solomons Island, Vanuatu, Fiji, and Norfolk Island

Using Nuclear Introns to Infer the Evolutionary History of the Understudied Australian Red Robins

John Malloy, Anna Kearns, Matthias Gobbert, Amy Driskell, Kevin Omland Introduction The Australasian red robins (genus Petroica) are commonly used as a textbook example of island speciation due to the radiation of the species outward from the Australian mainland (Mayr, Animal Species and Evolution, 1950). Although their common name sounds familiar, the Australian Robins are a distinct group not at all closely related to our American Robin. Traditionally, the evolutionary relationships within the genus have grouped the mainland Scarlet Robin (Petroica boodang) with the Pacific Robin (Petroica multicolor) which is found on nearby islands, such as Solomons Island, Vanuatu, Fiji, and Norfolk Island. However, recent molecular based phylogenies suggest this traditional view to be flawed. A mitochondrial DNA study by Christidis et al. (2011) puts the Scarlet Robin with the Flame and Pink Robins instead of with the Pacific Robin. In previous studies by the Omland lab, mitochondrial gene trees have been shown to be problematic in inferring species trees (Jacobsen and Omland, 2011). Therefore, here we test the validity of the traditional and mitochondrial phylogenies using nuclear DNA to infer a species tree of the Petroica genus .

Traditional Morphological Mitochondrial DNA Nuclear DNA Relationship Relationship Relationship Paciﬁc Robin 0.56 Scarlet Robin 0.57 Scarlet Robin

0.95 Flame Robin 1 Pink Robin Scarlet Robin Pink Robin Flame Robin 0.91 0.51 Red-Capped Red-Capped Red-Capped Robin 1 Robin 1 Robin

Pink Robin Paciﬁc Robin Paciﬁc Robin

P. macrocephala P. macrocephala Flame Robin Christidis et al. (2011). Analysis performed with MrBayes 3.1.1 Analysis performed with *BEAST 1.8.0 (Drummond et al. (Huelsenbeck and Ronquist, 2003). Branch labels are posterior 2012). Branch labels are posterior probabilities. probabilities.

Conclusions Range of the Pacific Robin The nuclear DNA results support the mitochondrial DNA study for the overall clades. In both analyses, the Scarlet, Flame, and Pink Robins are Solomons Is. together in one clade, and the Red- Capped and Pacific Robins are in a Petroica goodenovii (Red- Petroica multicolor Capped Robin) Vanuatu Fiji separate clade. (Pacific Robin – Norfolk Island Robin) Both analyses debunk the traditional view of Scarlet and Pacific Robins as Norfolk Is. sister taxa.

The nuclear DNA analysis puts the Scarlet and Pink Robins as sister taxa, whereas the mitochondrial analysis places the Scarlet Robin Petroica rodinogaster (Pink Robin) with the Flame Robin.

Further testing is necessary to infer a complete phylogeny of the genus Petroica.

Petroica boodang Acknowledgements (Scarlet Robin) This work was funded, in part, by the UBM program at UMBC, an interdisciplinary biology and math training grant funded by the National Science Foundation (NSF) as Petroica phoenicea well as by an NSF grant to Dr. Kevin Omland. Results were obtained with the use of (Flame Robin) Range shown in red is the range of the family Petroicidae the UMBC High Performance Computing Facility, supported by NSF, with additional Map Source: Wikipedia http://en.wikipedia.org/wiki/Petroicidae substantial support by UMBC.