Genetic Diversity and Conservation Biology of Moringa Peregrina Populations in Sinai, Egypt
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Genetic diversity and conservation biology of Moringa peregrina populations in Sinai, Egypt Inauguraldissertation zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften (Dr. rer. nat.) der Mathematisch-Naturwissenschaftlichen Fakultät der Ernst-Moritz-Arndt-Universität Greifswald vorgelegt von Mohamed A. EL Dadamouny A. Sheteiwy geboren am 03.05.1981 in El Manzala, Ägypten Greifswald, März 2018 Dekan: Prof. Dr. Werner Weitschies 1. Gutachter : Prof. Dr. Christoph Oberprieler Universität Regensburg, Germany 2. Gutachter: Prof. Dr. Martin Schnittler Universität Greifswald, Germany Tag der Promotion: 21.08.2018. Dedication I dedicate my dissertation to my family. To my father, my mother, and to my wife Mrs. Riham Sheteiwy My daughters Sama, Hana, and Jana, and to the spirit of my grandmother Preface Since my undergraduate stage (1998–2002) at Faculty of Science in Ismailia – Egypt, I used to visit Sinai Peninsula for field work. I was attracted to the highly diverse flora of Sinai, which includes around 2000 plant species. Most of these species are medicinal plants that used in the folk medicine and considered as one of the income resources to the Bedouins in this interesting peninsula. One of these medicinal plants was Moringa peregrina, which was standing with Acacia species and Crataegus x Sinaica for several years facing the impacts of high aridity of the area and over collection. These threats led to a gap in the population dynamics. One of the common observations at that time was the poor recruitment due to the low establishment. I did my graduation project (2001–2002) on that point trying to increase the establishment rate with a decreasing of the mortality rate for the germinated seedlings. From 2002 to 2004, I worked within a project aimed to conserve the remaining populations. I got a great chance to understand more about the tree biology (e.g. phenological stages starting from budding and flowering time, fruiting, and etc.). From 2004–2009, I did also my M.Sc. project on the population dynamics of this valuable species. For that reason, its declining populations are located under threats of climate change and anthropogenic impacts. The response of the tree towards such external threats was unclear with a continuous missing in the recruitment and then a continuous decline in its population size. Establishing the conservation programs could help in mitigating the adverse impacts of these threats. But, the tree itself should have the capability to adapt and to overcome towards these external problems if the current populations are genetically diverse. Therefore, we aimed in my Ph.D. project to find a solution for the gap on its reproductive biology and genetic diversity within and among its populations that needed for conservation programs. As an outputs for all works done over the past years under the supervision of Prof. Martin Schnittler, four scientific papers (two accepted papers and two manuscripts) and one review are achieved and included in this cumulative thesis. List of publications included in this cumulative dissertation Peer-Reviewed Publications Dadamouny MA and Schnittler M (2016) Trends of climate with a rapid change in Sinai, Egypt. Journal of water and climate change 7(2):393–414. IWA doi: 10.2166/wcc.2015.215 Dadamouny MA, Unterseher M, König P, and Schnittler M (2016) Population performance of Moringa peregrina (Forssk.) Fiori (Moringaceae) at Sinai Peninsula, Egypt in the last decades: consequences for its conservation. Journal for Nature Conservation 34:65–74 Manuscripts Dadamouny MA and Schnittler M. Demographic and size structure status of the threatened populations of Moringa peregrina trees at Sinai Peninsula, Egypt. Mohamed Dadamouny, Anja Klahr, Franziska Eichhorn, and Martin Schnittler. Relictic populations of the endangered tree Moringa peregrina (Moringaceae) in Sinai, Egypt are still genetically diverse. 4 Genetic diversity and conservation biology of Moringa peregrina populations in Sinai, Egypt Contents Contents Page Title page - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 1 Defense committee - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 2 Dedication - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 3 Preface - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 4 Table of Contents - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 5 Chapter 1: Summary - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 7 1.1. Englisch Summary - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 7 1.2. German Summary - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 9 Chapter 2: Introduction - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 12 Background - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 12 1. Species identification - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 12 1.1. Taxonomic position of M. peregrina - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 12 1.2. Distribution of M. peregrina - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 12 1.3. Morphology of M. peregrina - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 13 1.4. Anatomy of M. peregrina - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 17 1.5. Phytochemical analyses of M. peregrina - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 18 1.6. Seed viability and germination of M. peregrina - - - - - - - - - - - - - - - - - - - - - - - - 18 1.7. Uses of M. peregrina - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 19 2. Problem identification and looking for solutions - - - - - - - - - - - - - - - - - - - - - - - - - - - - 19 3. Goals of the thesis - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 21 References - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 22 Chapter 3: Publications - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 24 3.1. Trends of climate with a rapid change in Sinai, Egypt - - - - - - - - - - - - - - - - - - - - - - - 25 Abstract - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 25 Introduction - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 25 Study area - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 27 Material and methods - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 29 Results - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 30 Discussion - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 36 Conclusion - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 41 References - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 42 Appendix I - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 47 Appendix II - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 50 3.2. Population performance of Moringa peregrina (Forssk.) Fiori (Moringaceae) at Sinai Peninsula, Egypt - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- - - - - - 52 Abstract - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 52 Introduction - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 52 Material and methods - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 54 Study area - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 54 Results - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 56 Discussion - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 63 5 Contents Genetic diversity and conservation biology of Moringa peregrina Conclusion - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 65 References - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 66 Appendix III - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 69 Appendix IV - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 71 3.3. Demographic and size structure status of the threatened populations of Moringa peregrina trees at Sinai Peninsula, Egypt - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -