DOCSLIB.ORG

Isolation and Characterisation of Substances from Royal Jelly Andreas Stocker

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Isolation and Characterisation of Substances from Royal Jelly Andreas Stocker Isolation and characterisation of substances from Royal Jelly Andreas Stocker To cite this version: Andreas Stocker. Isolation and characterisation of substances from Royal Jelly. Other. Université d’Orléans; Université technique de Munich, 2003. English. tel-00008586 HAL Id: tel-00008586 https://tel.archives-ouvertes.fr/tel-00008586 Submitted on 27 Feb 2005 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. THÈSE EN COTUTELLE PRESENTÉE A L’UNIVERSITÉ D’ORLÉANS POUR OBTENIR LE GRADE DE DOCTEUR-INGÉNIEUR DE L’UNIVERSITÉ D’ORLÉANS Discipline : Biophysique moléculaire PAR -STOCKER Andreas Isolation and characterisation of substances from Royal Jelly Soutenue le 26 septembre 2003 M. Rudi F. VOGEL Président de la Jury MEMBRES DU JURY : (Fonction) M. Eberhard BENGSCH Directeur de Thèse; Examinateur M. Jürgen POLSTER Directeur de Thèse; Examinateur M. André BRACK Examinateur M. Antonius KETTRUP Rapporteur M. Bernard HEUSCH Rapporteur Mme. Paule VASSEUR Rapporteur extérieur M. Friedrich LOTTSPEICH Rapporteur extérieur M. Helmut HORN Rapporteur extérieur Mme. Agnes H. HENSCHEN-EDMAN Membre invité Deutsch-Französische Doppelpromotion (Cotutelle de Thèse) Lehrstuhl für Biologische Chemie Centre de Biophysique Moléculaire Lehrstuhl für Technische Mikrobiologie Isolation and characterisation of substances from Royal Jelly Andreas Stocker Vollständiger Abdruck der von der Fakultät Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt der Technischen Universität München und der Faculté des Sciences, Université d’Orléans zur Erlangung des akademischen Grades eines Doktor-Ingenieurs genehmigten Dissertation. Vorsitzender/Président du Jury: Univ.-Prof. Dr.rer.nat.habil. Rudi F. Vogel Prüfungskommission/ Jury de Thèse de Doctorat: Prüfer/Examinateurs: 1. Univ.-Prof. Dr.rer.nat. Dr.rer.nat.habil. Jürgen Polster 1. Dr.Sc.habil. Eberhard Bengsch (Directeur de Thèse) HDR, CNRS, Centre de Biophysique Moléculaire, Orléans (Frankreich) 2. Dr.Sc.habil. André Brack (Directeur de Recherche) HDR, CNRS, Centre de Biophysique Moléculaire, Orléans (Frankreich) Gutachter/Rapporteurs: 3. Univ.-Prof. Dr.rer.nat. Dr.h.c. (RO) Antonius Kettrup 4. Dr.Sc.habil. Bernard Heusch (Directeur de Recherche) HDR, CNRS, Direction des Relations Internationales, Paris (Frankreich) Externe Gutachter/ Rapporteur extérieurs: 5. Prof. Dr.Sc.habil. Paule Vasseur, U.F.R. Sciences Fondamentales et Appliquées, Université Metz (Frankreich) 6. Dr.rer.nat. Dr.sc.agr. Helmut Horn, Universität Hohenheim-Stuttgart Landesanstalt für Bienenkunde 7. Univ.-Doz. Dr.phil. Dr.med.habil. Friedrich Lottspeich Max-Planck-Institut für Biochemie, Martinsried Membre invité: 8. Prof. Dr.med. Agnes H. Henschen-Edman Departement of Biological Sciences, Molecular Biology and Biochemistry, University of California, Irvine (USA) Die Dissertation wurde am 24.6.2003 bei der Technischen Universität München eingereicht und durch die Fakultät Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt, sowie durch die Faculté des Sciences, Université d’Orléans am 7.8.2003 angenommen. III MRJP5 MRJP3 MRJP1 MRJP3 MRJP2 MRJP2 MRJP3 Central topic of this work: Major royal jelly proteins (MRJP’s), its polymorphism and proteolytic derivates (detailed information in fig. 6.5.1; page 147 and fig. 6.6.3; page 156). Preface IV Preface This dissertation was completed as the first contracted German-French joint research project (Cotutelle de Thèse) of the Technische Universität München and the Centre de Biophysique Moléculaire, CNRS UPR 4301 affiliated to the University of Orléans. Substances and results were produced in about equal parts in Germany and in France. The multidisciplinary structure of the dissertation demanded that the dissertation was integrated in several institutes thereby enabling a multifaceted execution of the theme. The joint research with the Centre de Biophysique Moléculaire, CNRS Orléans, permitted, among other procedures, the characterisation of peptides and proteins extracted from royal jelly by a multitude of physical-chemical and biochemical methods of separation. Particular peptides were produced by the Merryfield Synthesis for antibacterial tests and were characterised by NMR studies. The dissertation originated from a Diplomarbeit at the TU München in Weihenstephan with the title: Antibacterial effects of proteins fractions extracted from Royal Jelly. This microbiological and protein-analytical research became the point of departure for the following dissertation. At the TU München the research was mainly executed in the chairs of Technische Mikrobiologie, of Biologische Chemie and of Obstbau, and in the Institut für Mikrobiologie (FML), and with the laboratory of Proteomics. At the GSF München, in the Institut für Ökologische Chemie numerous and different tests as the analysis of trace elements and the characterisation of sequences of peptides extracted from royal jelly were achieved with NMR spectroscopy. In cooperation with the Universität Hohenheim, Stuttgart (Landesanstalt für Bienenkunde) the botanical origins of the royal jelly samples were determined by analysis of the pollen morphology. Preface V In cooperation with the Max-Planck-Institut für Biochemie, Martinsried (laboratory of Proteinanalytik) proteins were characterised with MALDI-MS and Edman- degradation. Antiviral properties of royal jelly against Coxsackie viruses were tested in the laboratory of Virusforschung of the same institute. The beginning phase of the Cotutelle de Thèse of the TU München was supported through organisation and financing by the Centre de Coopération Universitaire Franco-Bavarois (CCUFB), München. The research was also supported financially by the Université Franco-Allemande (UFA), Sarrebruck, Allemagne (Robert-Bosch-Stiftung). Content VI Content page 1. General introduction and objectives 1 2. Seasonal variation of microbiological activity and botanical origin of royal jelly samples 9 2.1 Introduction 10 2.2 Methods and Materials 11 2.3 Results 15 2.4 Various geographical and seasonal origins 38 2.5 Discussion 39 3. Microbiological effects 45 3.1 Inhibitory potential against nosocomial pathogen bacteria 45 3.2 Visualisation of flavan-3-ols in bacteria and lymphocytes - inhibitory effects of flavan-3-ols in bacteria and accumulation in lymphocytes 62 3.3 Extension of the investigated anti-microbial activities on viral infections 69 4. Contents of trace elements and homeostatic effects 73 4.1 Introduction 73 4.2 Methods and Materials 75 4.3 Results 77 4.4 Discussion 87 5. Multielement stable isotope ratio analysis – authenticity and origin 92 5.1 Introduction 92 5.2 Methods and Materials 95 5.3 Results 97 5.4 Discussion 106 6. Analysis, isolation and characterisation of proteins and peptides – detailed investigation of protein components 112 6.1 Gel filtration, ultra filtration and ion exchange chromatography 125 Content VII 6.2. Analytical C8 RP-HPLC and N-terminal sequencing (Edman degradation) 128 6.3 Analytical 2-D electrophoresis of selected C8 RP-HPLC peaks: from HPLC to 2-DE gel 136 6.4. Analysis of C8 RP-HPLC protein peaks with electrospray ionisation- mass spectrometry (ESI-MS) 141 6.5 Micropreparative 2-D electrophoresis and N-terminal sequencing (Edman degradation) 147 6.6. MALDI-MS characterisation and micropreparative 2-D electrophoresis 153 7. General discussion, final remarks and perspectives 158 8. Summary, Sommaire, Zusammenfassung 165 9. References 175 Acknowledgements Abbreviations VIII Abbreviations AU Absorbance Units APS ammonium persulphate cDNA copy desoxiribonucleic acid CF-IRMS continuous flow isotope ratio mass spectrometry CHAPS cholamidopropyl-dimethylammonio-propanesulfonate 1D one dimensional 2D two dimensional Da Dalton DMACA p-dimethyl-aminocinnamaldehyd DTT Dithiothreitol DSM Deutsche Stammsammlung für Mikroorganismen und Zellkulturen ESI-MS electrospray ionization mass spectrometry RJ Royal Jelly 10-HDA 10-hydroxy-∆2-decenoic acid HPLC High Performance Liquid Chromatography ICP-MS inductive coupled plasma mass spectrometry IEF isoelectric focussing IPG immobilized pH-gradients IPG-Dalt two dimensional electrophoresis with immobilized pH-gradients kDa kiloDalton LMW low molecular weight MIC minimal inhibitory concentration Abbreviations IX MALDI-MS matrix assisted laser desorption ionisation mass spectrometry MR relative molecular weight m/z mass/charge ratio NMR nuclear magnetic resonance spectrometry OES optical emission spectrometry pI isoelectric point ppb parts per billion ppm parts per million RT room temperature SDS Sodium n-Dodecyl Sulphate ssp. subspecies %T proportion of total acrylamide TEMED N,N,N’,N’-tetramethylendiamin TI-MS thermal ionisation mass spectrometry TMW Technische Mikrobiologie Weihenstephan TOF time of flight Tris Tris(hydroxymethyl)-aminomethan w/v mass per volume v/v volume per volume 1 General introduction and objectives 1 1 General introduction and objectives Royal jelly is a bee product from the hypopharyngeal, mandibular and postcerebral glands of young worker bees. It is produced under partial digestion of essentially
Load more

Recommended publications
  • "National List of Vascular Plant Species That Occur in Wetlands: 1996 National Summary."
    Intro 1996 National List of Vascular Plant Species That Occur in Wetlands The Fish and Wildlife Service has prepared a National List of Vascular Plant Species That Occur in Wetlands: 1996 National Summary (1996 National List). The 1996 National List is a draft revision of the National List of Plant Species That Occur in Wetlands: 1988 National Summary (Reed 1988) (1988 National List). The 1996 National List is provided to encourage additional public review and comments on the draft regional wetland indicator assignments. The 1996 National List reflects a significant amount of new information that has become available since 1988 on the wetland affinity of vascular plants. This new information has resulted from the extensive use of the 1988 National List in the field by individuals involved in wetland and other resource inventories, wetland identification and delineation, and wetland research. Interim Regional Interagency Review Panel (Regional Panel) changes in indicator status as well as additions and deletions to the 1988 National List were documented in Regional supplements. The National List was originally developed as an appendix to the Classification of Wetlands and Deepwater Habitats of the United States (Cowardin et al.1979) to aid in the consistent application of this classification system for wetlands in the field.. The 1996 National List also was developed to aid in determining the presence of hydrophytic vegetation in the Clean Water Act Section 404 wetland regulatory program and in the implementation of the swampbuster provisions of the Food Security Act. While not required by law or regulation, the Fish and Wildlife Service is making the 1996 National List available for review and comment.
    [Show full text]
  • Seed Ecology Iii
    SEED ECOLOGY III The Third International Society for Seed Science Meeting on Seeds and the Environment “Seeds and Change” Conference Proceedings June 20 to June 24, 2010 Salt Lake City, Utah, USA Editors: R. Pendleton, S. Meyer, B. Schultz Proceedings of the Seed Ecology III Conference Preface Extended abstracts included in this proceedings will be made available online. Enquiries and requests for hardcopies of this volume should be sent to: Dr. Rosemary Pendleton USFS Rocky Mountain Research Station Albuquerque Forestry Sciences Laboratory 333 Broadway SE Suite 115 Albuquerque, New Mexico, USA 87102-3497 The extended abstracts in this proceedings were edited for clarity. Seed Ecology III logo designed by Bitsy Schultz. i June 2010, Salt Lake City, Utah Proceedings of the Seed Ecology III Conference Table of Contents Germination Ecology of Dry Sandy Grassland Species along a pH-Gradient Simulated by Different Aluminium Concentrations.....................................................................................................................1 M Abedi, M Bartelheimer, Ralph Krall and Peter Poschlod Induction and Release of Secondary Dormancy under Field Conditions in Bromus tectorum.......................2 PS Allen, SE Meyer, and K Foote Seedling Production for Purposes of Biodiversity Restoration in the Brazilian Cerrado Region Can Be Greatly Enhanced by Seed Pretreatments Derived from Seed Technology......................................................4 S Anese, GCM Soares, ACB Matos, DAB Pinto, EAA da Silva, and HWM Hilhorst
    [Show full text]
  • Complementary and Alternative Medicine
    Complementary and Alternative Medicine Policy Number: Original Effective Date: MM.12.013 01/01/2014 Line(s) of Business: Current Effective Date: HMO; PPO 11/17/2017 Section: Miscellaneous Place(s) of Service: Outpatient I. Description Complementary and alternative medicine (CAM), also called non-traditional medicine, is a group of diverse medical and healthcare systems, practices and products that are not typically considered to be a part of traditional Western medicine (i.e., conventional medicine). This policy addresses services performed by CAM providers such as naturopaths, chiropractors and acupuncturists. Complementary medicine generally refers to using a non-traditional approach together with conventional medicine. Alternative medicine refers to using a non-traditional approach in place of conventional medicine. Various CAM assessments and therapies are supported by some degree of scientific evidence and are intended to reduce disease-based symptoms and to improve health outcomes. However, due to a lack of well-designed scientific studies and/or peer reviewed literature many CAM therapies have not been shown to improve health outcomes over conventional therapies. II. Criteria/Guidelines A CAM service or procedure is covered (subject to Limitations and Administrative Guidelines) when all of the following criteria are met: A. It meets the definition of medical necessity as specified in Hawaii Revised Statutes (HRS) Section 432: 1. It is for the purpose of treating a medical condition. 2. It is the most appropriate delivery or level of service, considering potential benefits and harms to the patient; 3. It is known to be effective in improving health outcomes; provided that: a. Effectiveness is determined first by scientific evidence; b.
    [Show full text]
  • The Determination of Complementary and Alternative Medicine Use in Patients Presenting at the Emergency Room
    Complementary Therapies in Clinical Practice 31 (2018) 164e169 Contents lists available at ScienceDirect Complementary Therapies in Clinical Practice journal homepage: www.elsevier.com/locate/ctcp The determination of Complementary and Alternative Medicine use in patients presenting at the emergency room * Zeliha Koç a, ,Tugba Çınarlı b a Ondokuz Mayıs University, Health Science Faculty, Samsun, Turkey b Ondokuz Mayıs University, Health Services Vocational College, Samsun, Turkey article info abstract Article history: Objective: The aim of this study was to determine CAM use in patients presenting at the emergency Received 13 September 2017 room. Received in revised form Method: This cross-sectional study was conducted with the participation of 385 patients presenting at 12 November 2017 the emergency room. Data was collected with a questionnaire between the dates 02.01.2016e31.03.2016. Accepted 1 February 2018 Results: The reasons for the presentation at the emergency room were found to be stomach ache (17.2%), vomiting nausea (14.8%), headache (11.2%), shortness of breath (10.9%), and urinary problems (9.6%). 94% Keywords: of the patients presenting at the emergency room were found to use CAM methods with the methods Emergency room Patients used being prayer (82.3%), herbal medicine/tea (48.6%), and diets supplementary (9.4%). 80.9% of the Complementary and Alternative Medicine patients were found not to share their CAM usage with health professionals. Conclusion: In order to ensure patient safety and prevent patients from coming to harm, it is thought that encouraging patients to share their CAM use with health care professionals is very important. © 2018 Elsevier Ltd.
    [Show full text]
  • Capital of Romanian Beekeeping and Apitherapy
    Capital of Romanian beekeeping and apitherapy Bucharest became the capital of the Romanian beekeeping and Apitherapy from October 11th to 14th by hosting the XII National Congress of Apitherapy with international participation. The Congress was organized by the Romanian Society of Apitherapy (R.S.A.) in partnership with IRDB (Institute of Research-Development in Beekeeping) and FIITAE Apimondia (Foundation of the International Institute of Technology and Apiculture economy) and brought together physicians, pharmacists, beekeepers, biologists, biochemists, engineers and businessmen from many countries of the world. The diverse theme of Congress was supported by oral presentations and posters, scientific debates, free conferences for pupils, students and pensioners, workshops, as well as an exhibition with the sale of apiculture products open to the general public. We selectively mention from the works presented at the Congress, based on section framing: • The history of beekeeping in Romania - Beekeeping, traditional occupation – Gabriela VLĂSCEANU, Iuliana CRIŞAN (Bucharest, Romania) • Melifero-Medicinal Plants - The melifera base in Romania and its importance for Apitherapy – Gabriela VLĂSCEANU (Bucharest, Romania) • Quality of apiculture products - A better quality of apiculture products through the application of new procedures and treatments to combat the parasite Varroa destructor – Adrian SICEANU, Eliza CĂUIA, Gabriela Oana VIŞAN, Dumitru CĂUIA (Bucharest, Romania) - The incidence of antibiotics in apiculture products. Case study
    [Show full text]
  • Complementary and Alternative Medicine Table of Contents Related Coverage Resources
    Medical Coverage Policy Effective Date ............................................. 2/15/2021 Next Review Date ....................................... 2/15/2022 Coverage Policy Number .................................. 0086 Complementary and Alternative Medicine Table of Contents Related Coverage Resources Overview.............................................................. 1 Acupuncture Coverage Policy .................................................. 1 Atherosclerotic Cardiovascular Disease Risk General Background ........................................... 3 Assessment: Emerging Laboratory Evaluations Medicare Coverage Determinations .................. 36 Attention-Deficit/Hyperactivity Disorder (ADHD): Coding/Billing Information ................................. 37 Assessment and Treatment References ........................................................ 39 Autism Spectrum Disorders/Pervasive Developmental Disorders: Assessment and Treatment Biofeedback Chiropractic Care Drug Testing Hyperbaric and Topical Oxygen Therapies Physical Therapy INSTRUCTIONS FOR USE The following Coverage Policy applies to health benefit plans administered by Cigna Companies. Certain Cigna Companies and/or lines of business only provide utilization review services to clients and do not make coverage determinations. References to standard benefit plan language and coverage determinations do not apply to those clients. Coverage Policies are intended to provide guidance in interpreting certain standard benefit plans administered by Cigna Companies. Please
    [Show full text]
  • Thermal Waters and Balneology in Bulgaria
    K. Bojadgieva, S. Dipchikova, A. Benderev, J. Koseva: BALNEOLOGY IN BULGARIA Chapter 1.3 THERMAL WATERS AND BALNEOLOGY IN BULGARIA K.Bojadgieva1, S.Dipchikova2, A.Benderev3 and J.Koseva2 1-Oil and Gas Production and Exploration, Ltd, 23 Sitnjakovo blvd., 1505 Sofia 2-Specialized hospital for physiotherapy and rehabilitation, 2B Ovcha kupel,1608 Sofia 3-Geological Institute, Bulgarian Academy of Sciences, Bl.24, G.Bonchev str.,1113 Sofia INTRODUCTION seases are treated using scientific methods and programs, confirmed by a long-term Bulgaria has a variety of mineral waters o professional experience. with temperatures up to 100 C. Thermal wa- The economical and social changes in ters with high alkalinity and low level of our country during the transition period to- TDS are predominant. The country is situa- wards the free market economy led to a dra- ted at the southern part of the Balkan Penin- stic decrease in the governmental subsidies sula and is an heir of ancient civilizations. for balneology, to a deterioration of some of There are extremely good bio-climatic the resorts and even to a closure of some of resources which combined with the existing them. The Bulgarian spas are under the Mi- ancient Mediterranean traditions in thermal nistry of Health governance and their spon- water use, provide an have been based for soring, management and exploitation is go- the balneological development in the coun- ing to be done according to the new laws try. A number of big spa resorts have deve- and regulations in the country. loped on places of old Thracian or Roman In most of Bulgarian spas the thermal residential areas, like: Sandanski, Kjusten- waters are used partially and ineffectively.
    [Show full text]
  • Geranium Pratense
    Geranium pratense Family: Geraniaceae Local/common names: Meadow Cranesbill, Palto (Ladakh) Trade name: Geranium oil Profile: Geranium pratense, a high altitude member of the family Geraniaceae and grows in meadows and roadsides ranging from Europe to the Himalayas in north Asia. It is a native of Europe. The plant has saucer-shaped flowers in shades of white, blue or violet in early to mid-summer. The roots of the plant have medicinal properties and used as a vulnerary. The plant is also used in the perfume industry. Habitat and ecology: The plant is found on open slopes and occupies moist places and stony wet slopes adjoining river streams and rivulets. It occurs from Western Europe to Western China. The Central European G. pretense is confined to relatively warm areas and is absent in higher mountain regions. In India, the species is available in Ladakh (Jammu and Kashmir), Lahaul and Spiti, Pangi, Kinnaur (Himachal Pradesh), Joshimath (Uttarakhand), Sikkim and Tawang (Arunchal Pradesh). Morphology: It is a perennial garden herb. The leaves are divided into five or seven lobes, which are also lobed and toothed. The stem leaves are paired and gradually decrease in size towards the top, while the stalks become shorter. The flowers are up to 4.5 cm in diameter, blue to bluish-purple in colour and mounted on flower stalks. The immature fruit and its stalk are reflexed. Distinguishing features: The inflorescence bears glandular hairs and the characteristically large, blue to bluish-purple flowers are positioned in a more or less horizontal direction with respect to the stem.
    [Show full text]
  • Taxonomic Revision of Geranium Subsect. Mediterranea (Geraniaceae)
    Systematic Botany (2007), 32(1): pp. 93–128 # Copyright 2007 by the American Society of Plant Taxonomists Taxonomic Revision of Geranium Subsect. Mediterranea (Geraniaceae) CARLOS AEDO,1,3 MIGUEL A´ .GARCI´A,1 MARI´A L. ALARCO´ N,1 JUAN J. ALDASORO,1 and CARMEN NAVARRO2 1Real Jardı´n Bota´nico, Consejo Superior de Investigaciones Cientı´ficas, Plaza de Murillo 2, 28014 Madrid, Spain; 2Departamento de Biologı´a Vegetal II, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Spain 3Author for correspondence ([email protected]) Communicating Editor: Gregory M. Plunkett ABSTRACT. Geranium subsect. Mediterranea (Geraniaceae) consists of ten species. The highest diversity of the group is located in the Caucasus and neighbouring areas of Turkey and Iran, with five endemic species. Other species reach western Europe and northwestern Africa. In contrast to the current literature, we consider G. montanum and G. ibericum subsp. jubatum to be synonyms of G. ibericum. A univariate morphometric study revealed some valuable quantitative characters useful for the identification of these species. Micromorphological features of pollen, stigmas, seeds, and mericarps were investigated by SEM. A new key is provided, as well as new and detailed descriptions. Geranium kurdicum is here illustrated for the first time. Eleven lectotypes are designated, and distribution maps are presented. Maximum parsimony and Bayesian analyses of chloroplastic trnL-trnF and ribosomal nuclear ITS regions suggest that sect. Mediterranea is monophyletic. Two clades are recovered, one including the annual species and other with the perennials, in which G. tuberosum (subsect. Tuberosa) emerges within a paraphyletic subsect. Mediterranea. RESUMEN. Geranium subsect. Mediterranea (Geraniaceae) esta´ formada por diez especies.
    [Show full text]
  • Complementary and Alternative Medicine
    Medical Policy: Complementary and Alternative Medicine # [Policy Number] Effective July 9, 2002 Revised: August 2005; November 1, 2011; April 16, 2012 COMPLEMENTARY AND ALTERNATIVE MEDICINE Policy Except as is described below, VCHCP does not cover alternative medicine treatments or interventions. Though not an all inclusive list, VCHCP does not cover the following alternative medicine interventions, because there is inadequate evidence in the peer-reviewed published medical literature of their effectiveness: Essiac • Diagnostic testing methods Fecal bacteriotherapy Fish oil (omega-3 fatty acids) applied kinesiology (AK) Flower essences chemical hair analysis Gerson therapy Greek cancer cure test Ginkgo biloba Iridology Gucosamine Live blood cell analysis Hoxsey method Micronutrient panel testing Hydrazein sulfate Antioxidant function testing Hydrogen peroxide, intravenous Ream’s testing Immunoaugmentive therapy Kava • Whole medical systems Kelley-Gonzales therapy Ayurveda Laetrile Homeopathy Lorenzo’s oil Macrobiotics Megavitamin therapy Naprapathy Milk thistle Naturopathy Mistletoe Polarity therapy MTH-68 Ozone therapy • Biologically-based practices Revici’s Guided Chemotherapy Actra-Rx Saw palmetto Antineoplastons 714-X Apitherapy Shark cartilage products Aromatherapy St. John’s wort Auto urine therapy Trichuris suis ova therapy Bilberry Valerian Black cohosh yohimbe Bovine cartilage products Cancell (Entelev) • Energy medicine Cat’s claw Acupressure Cellular therapy Biofield therapeutics
    [Show full text]
  • Bee Products and Oxidative Stress: Bioavailability of Their Functional Constituents
    Review Article Mod Appl Bioequiv Availab Volume 1 Issue 3 - June 2017 DOI: 10.19080/MABB.2017.01.555565 Copyright © All rights are reserved by Otilia Bobiş Bee Products and Oxidative Stress: Bioavailability of Their Functional Constituents Bobis O1*, Bonta V1, Varadi A2, Strant M2 and Dezmirean D3 1University of Agricultural Sciences and Veterinary Medicine, Romania 2Health with CasaBIO Asssociation, Romania 3Department of Apiculture and Sericiculture, University of Agricultural Sciences and Veterinary Medicine, Romania Submission: June 05, 2017; Published: June 26, 2017 *Corresponding author: Otilia Bobiş, Life Science Institute “King Michael I of Romania”, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania, Email: Keywords: Bee products; Oxidative stress; Bioavailability; Chemical composition Bee Products Introduction geographic distribution. Honey also depend on these factors, Many times, what we live, what we eat is reflected in our as bees collect nectar as main source for honey producing. For health. Natural foods or natural nutritive supplements must a better knowledge of honey properties, one must know its have an important place in human diet. Talking about natural botanical origin. Depending on the raw material from which bees products, implicitly we think in bee products. Main hive products produce honey, we can distinguish: nectar honey and honeydew are honey, bee collected pollen, propolis, royal jelly, wax and bee honey. Floral honey comes from the nectar of several plants, and venom. These products have different roles in bee colony and in if one is predominant we have monofloral honey, and if a mixture human health. of pollen and nectar is present, without the net dominance of a For men, honey, royal jelly and bee pollen are foods that may particular plant, we have multifloral honey.
    [Show full text]
  • Apresentação Do Powerpoint
    Are the integrative practices recently incorporated into the Brazilian public healthcare system supported by evidence from Cochrane Reviews? Riera R1,2, Dittrich NH1, Pacheco RL1,3, Latorraca COC1, Martimbianco ALC3,4, Atallah AN1,5 1 Universidade Federal de São Paulo (Unifesp), Brazil 2 Hospital Sírio-Libanês, Brazil 3 Centro Universitário São Camilo, Brazil 4 Universidade Metropolitana de Santos (UNIMES), Brazil 5 Cochrane Brazil, Brazil The Problem Methods In March 2018, the Brazilian Ministry of Health announced 10 new integrative practices as part of the list of procedures available in the Brazilian public healthcare system, Sistema Único de Saúde (SUS). SUS, a free and universal system, has a limited budget, and all interventions should be fairly supported by good Apitherapy quality studies before its implementation. We summarized the evidence from Cochrane Reviews (CRs) Aromatherapy about these 10 interventions [1]. Bioenergetics Family constellation Key Results Chromotherapy Clay therapy Search was carried out in March 2018: 189 reviews. 16 CRs were included. Only 4 of the 10 integrative Hypnotherapy practices (apitherapy, aromatherapy, hypnotherapy and ozone therapy). For six integrative Hand imposition interventions (bioenergetics, family constellation, chromotherapy, geotherapy, flower therapy, and Ozone therapy hand imposition), no CR was found. The certainty of evidence ranged from unknown to high. The high- Flower therapy certainty evidence: honey dressings for partial thickness burns, honey for acute cough in children, and venom immunotherapy for systematic reaction to an insect sting. Almost the totality of the new interventions incorporated by Brazilian Ministry of Health in 2018 are not support by evidence from Cochrane Reviews. Sensitive search strategy Table 1.
    [Show full text]