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Immune Cell Isolation & Culture

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Immune Cell Isolation & Culture Immune Cell Isolation & Culture Immune Cell Isolation & Culture Immune cell isolation and culture are necessary for both basic research aimed at investigating the functions of different immune cell types and for expanding specific immune cell populations for therapeutic purposes. Bio-Techne is focused on providing the highest quality reagents for isolating, activating, differentiating, and culturing immune cells. From natural killer (NK) cells and macrophages to dendritic cells and T cells, we offer kits for both cell enrichment and cell differentiation or expansion, along with cell culture media, fetal bovine serum, and an unparalleled selection of recombinant bioactive proteins and antibodies. In addition to our reagents for basic research, we continue to expand our selection of innovative tools for ex vivo cell manufacturing, making it easier for researchers to transition their research from discovery to the clinic. Our portfolio now includes GMP-grade recombinant proteins and GMP-manufactured custom cell separation and CD3/CD28 T cell activation kits, which both utilize non-magnetic Cloudz™ dissolvable microparticle technology. Contents Introduction to Immune Cell Isolation & Culture ......................................................................................................................................2 MagCellect™ Cell Selection Kits & Reagents .............................................................................................................................................3 T Cell Enrichment Columns ........................................................................................................................................................................4 CellXVivo™ Kits for Immune Cell Differentiation or Expansion .................................................................................................................4 Cell Culture Media .......................................................................................................................................................................................5 Classic Fetal Bovine Serum ........................................................................................................................................................................6 Recombinant Cytokines for Immune Cell Differentiation and Expansion ..............................................................................................7 ProDots® Proteins ..................................................................................................................................................................................... 13 Animal-Free™ and Animal Component-Free Process (ACFP) Recombinant Proteins ............................................................................................................................................................................. 13 GMP-grade Immune Cell Culture Reagents ............................................................................................................................................ 14 Cloudz™ Cell Selection Kits ...................................................................................................................................................................... 14 GMP-grade Recombinant Proteins .......................................................................................................................................................... 15 GMP Cloudz™ T Cell Activation Kit – CD3/CD28 .................................................................................................................................... 15 Custom Services ...................................................................................................................................................................................... 15 Learn more | rndsystems.com/immunecellculture 2 MagCellect™ Cell Selection Kits & Reagents Isolation or enrichment of a specific cell type of interest from a heterogeneous cell population is frequently a critical first step in immunology research for obtaining a pure population of cells for further characterization. R&D Systems® MagCellect Cell Selection Kits are designed to enrich for specific cell populations based on either a negative or posi- tive selection principle. Features of the kits include: • Beads with a high binding capacity • Small beads in ferrofluid that have no magnetic memory and will not induce cell damage • Cells can be separated to a very high purity in minutes MagCellect Human Cell Isolation Kits Catalog # A B Human B Cell Isolation Kit MAGH103 105 105 Human CD14+ Cell Isolation Kit MAGH105 Human CD3+ T Cell Isolation Kit MAGH101 Human Naïve CD4+ T Cell Isolation Kit MAGH115 104 104 Human CD4+ T Cell Isolation Kit MAGH102 Human Memory CD4+ T Cell Isolation Kit MAGH116 CD14 CD14 + + Human CD4 CD25 Regulatory T Cell Isolation Kit MAGH104 103 103 Human CD8+ T Cell Isolation Kit MAGH112 Human Natural Killer Cell Isolation Kit MAGH109 0 0 MagCellect Mouse Cell Isolation Kits Catalog # -103 -103 Mouse B Cell Isolation Kit MAGM204 -103 0 103 104 105 -103 0 103 104 105 Mouse CD3+ T Cell Isolation Kit MAGM201 CD19 CD19 Mouse Naïve CD4+ T Cell Isolation Kit MAGM205 A B Mouse CD4+ T Cell Isolation Kit MAGM202 5 5 Mouse Memory CD4+ T Cell Isolation Kit 10 MAGM206 10 Mouse Naïve CD8+ T Cell Isolation Kit MAGM207 Mouse CD4+CD25+ Regulatory T Cell Isolation Kit MAGM208 104 104 Mouse CD8+ T Cell Isolation Kit MAGM203 Mouse Natural Killer Cell Isolation Kit MAGM210 CD14 CD14 103 103 MagCellect Rat Cell Isolation Kits Catalog # Rat B Cell Isolation Kit MAGR303 0 0 Rat CD3+ T Cell Isolation Kit MAGR301B Rat CD4+ T Cell Isolation Kit MAGR302B -103 -103 -103 0 103 104 105 -103 0 103 104 105 MagCellect Plus Human Kits Catalog # CD19 CD19 Human CD45+ Cell Isolation Kit MAGH125 Isolation of Human CD19+ B Cells from PBMCs using the MagCellect Human B Cell Isolation Kit. Human PBMCs MagCellect Reagents Catalog # were prepared as described in the cell preparation section of the MagCellect Human B Cell Isolation Kit (R&D Systems, MagCellect Magnet MAG997 Catalog # MAGH103). Cells were stained both before (A) MagCellect Streptavidin Ferrofluid MAG999B and after (B) B cell isolation with the MagCellect Kit using a PE-conjugated Mouse Anti-Human CD19 Monoclonal Antibody (R&D Systems, Catalog # FAB4867P) and an APC- conjugated Mouse Anti-Human CD14 Monoclonal Antibody (R&D Systems, Catalog # FAB3832A). Learn more | rndsystems.com/magcellect 3 T Cell Enrichment Columns In addition to our MagCellect Kits, Bio-Techne also offers human and mouse T cell enrichment columns from our R&D Systems brand that are designed to enrich for a specific T cell population from a mononuclear cell suspension. Human T Cell Enrichment Columns Catalog # Mouse T Cell Enrichment Columns Catalog # Human CD3+ T Cell Enrichment Column HTCC Mouse CD3+ T Cell Enrichment Column MTCC Human CD4+ T Cell Enrichment Column HCD4C-1000 Mouse CD4+ T Cell Enrichment Column MCD4C-1000 Human CD4+ T Cell Enrichment Column, Mini HCD43 Mouse CD4+ T Cell Enrichment Column, Mini MCD43 Human CD4+/CD45RA- Memory T Cell Enrichment Column HCD42 Mouse CD8+ T Cell Enrichment Column MCD8C-1000 Human CD4+/CD45RO- Naïve T Cell Enrichment Column HCD41 Human CD8+ T Cell Enrichment Column HCD8C-1000 Human CD8+/CD45RA- Memory T Cell Enrichment Column HCD45 Human CD8+/CD45RO- Naïve T Cell Enrichment Column HCD44 CellXVivo™ Kits for Immune Cell Differentiation or Expansion R&D Systems CellXVivo Immune Cell Differentiation or Expansion Kits contain optimized combinations of the highest quality proteins and antibodies, along with simple, reproducible protocols for the differentiation or expansion of den- dritic cells, macrophages, T helper cell subtypes, regulatory T cells, B cells, natural killer cells, or cytokine-induced killer (CIK) cells. Benefits of the kits include: • Conveniently packages all reagents necessary for immune cell differentiation or expansion • Guaranteed to produce consistent, reliable results • Cost-effective and efficient A 104 B 104 C 104 D 104 0.9% 0.1% 49.1% 0.1% 0.5% 0.1% 47.6% 0.1% 103 103 103 103 102 102 102 102 IL-17 IL-17 IL-17 IL-17 101 101 101 101 98.5% 0.5% 50.6% 0.1% 99.4% 0.1% 51.9% 0.3% 100 100 100 100 100 101 102 103 104 100 101 102 103 104 100 101 102 103 104 100 101 102 103 104 IL-4 IL-4 IFN-γ IFN-γ Intracellular Cytokine Staining of Mouse Th17 Cells Following Differentiation using the CellXVivo Mouse Th17 Cell Differentiation Kit. Mouse naïve CD4+ T cells without (A, C) or with (B, D) a 5-day differentiation using the reagents provided in the CellXVivo Mouse Th17 Differentiation Kit (R&D Systems, Catalog # CDK017) were stimulated with Cell Activation Cocktail (Tocris, Catalog # 5476) and stained with a PE-conjugated Rat Anti-Mouse IL-17/IL-17A Monoclonal Antibody (Novus Biologicals, Catalog # NBP1-72027) and either an (A, B) APC- conjugated Rabbit Anti-Mouse IL-4 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-52656) or an (C, D) APC-conjugated Rat Anti-Mouse IFN-g Monoclonal Antibody (R&D Systems, Catalog # IC485A) and detected by flow cytometry. Quadrants were set based on staining with the appropriate isotype controls. Human CellXVivo Differentiation or Expansion Kits Catalog # Mouse CellXVivo Differentiation Kits Catalog # Human B Cell Expansion Kit CDK005 Mouse Dendritic Cell Differentiation Kit CDK008 Human CIK Expansion Kit CDK014 Mouse Th1 Cell Differentiation Kit CDK018 Human M1 Macrophage Differentiation Kit CDK012 Mouse Th2 Cell Differentiation
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