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Effect of Enterococcus Faecium M-74 Strain on Selected Blood and Production Parameters of Laying Hens Marcela Capcarova

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Effect of Enterococcus Faecium M-74 Strain on Selected Blood and Production Parameters of Laying Hens Marcela Capcarova Effect of Enterococcus faecium M-74 strain on selected blood and production parameters of laying hens Marcela Capcarova To cite this version: Marcela Capcarova. Effect of Enterococcus faecium M-74 strain on selected blood and production parameters of laying hens. British Poultry Science, Taylor & Francis, 2010, 51 (05), pp.614-620. 10.1080/00071668.2010.513961. hal-00638521 HAL Id: hal-00638521 https://hal.archives-ouvertes.fr/hal-00638521 Submitted on 5 Nov 2011 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. British Poultry Science For Peer Review Only Effect of Enterococcus faecium M-74 strain on selected blood and production parameters of laying hens Journal: British Poultry Science Manuscript ID: CBPS-2009-128.R2 Manuscript Type: Original Manuscript Date Submitted by the 11-Feb-2010 Author: Complete List of Authors: Capcarova, Marcela; Slovak Agricultural University, Animal Physiology Biochemistry, Fats and fatty acids, Haematology, Laying hens, Keywords: Probiotics E-mail: [email protected] URL: http://mc.manuscriptcentral.com/cbps Page 1 of 20 British Poultry Science CBPS-2009-128 Edited Lewis July 2010, MacLeod August 2010 1 2 3 1 Effects of Enterococcus faecium M 74 strain on selected blood and production 4 5 6 2 parameters of laying hens 7 8 3 9 10 1 11 4 M. CAPCAROVA, L. CHMELNICNA , A. KOLESAROVA, P. MASSANYI AND J. 12 13 5 KOVACIK 14 15 16 6 Department of ForAnimal Physiology, Peer Slovak Review University of Agricult Onlyure in Nitra, Tr. A. Hlinku 2, 17 1 18 7 SK – 949 76, Slovak Republic Department of Poultry Science and Small Animal Husbandry, 19 20 8 Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, SK – 949 76, Slovak Republic 21 22 23 9 24 25 10 26 11 27 Running title: Probiotics in poultry nutrition 12 28 29 13 30 14 31 15 32 16 33 17 34 18 35 36 19 37 20 38 21 39 22 40 23 41 42 24 43 25 44 26 45 27 Correspondence to: M. Capcarova, Department of Animal Physiology, Slovak University of 46 28 Agriculture in Nitra, Tr. A. Hlinku 2, SK – 949 76, Slovak Republic. 47 29 E-mail: [email protected] 48 49 30 50 51 31 52 53 54 55 56 57 58 59 60 Accepted for publication: 17 th March 2010 E-mail: [email protected] URL: http://mc.manuscriptcentral.com/cbps British Poultry Science Page 2 of 20 2 1 2 3 32 Abstract: 1. The aim of the present study was to evaluate the functional efficiency of a 4 5 6 33 probiotic strain, Enterococcus faecium M 74, in the feed on selected biochemical, 7 8 34 haematological and production parameters in ISA Brown hens. 9 10 35 2. Feed in the experimental group was enriched with a probiotic preparation containing of 11 12 9 13 36 5x10 viable Enterococcus faecium M 74 per g. Blood samples were collected during the egg 14 15 37 laying period at 5 (w5), 25 (w25) and 45 (w45) weeks of production. Body weight, rate of lay 16 For Peer Review Only 17 18 38 and egg weight were recorded every 4 weeks during the 48-week laying period. 19 20 39 3. Significantly lower concentrations of total cholesterol and total lipids in blood plasma were 21 22 40 observed in the experimental group at all sampling times compared with their respective 23 24 25 41 controls. Concentrations of triglycerides did not differ. Significantly lower concentrations of 26 27 42 plasma calcium were found in the experimental group at w5 and w45. Concentrations of 28 29 43 inorganic phosphorus in the experimental group were significantly higher at w25 but 30 31 32 44 significantly lower at w45. Erythrocyte count was significantly higher in the experimental 33 34 45 group at w25 and w45 when compared with controls. Leucocyte counts were significantly 35 36 46 lower in the experimental group at all sampling times. Significantly lower values of 37 38 39 47 haematocrit at w5 and w45 were observed in the experimental group than controls. Body 40 41 48 weight, number of eggs and average egg weight were not significantly affected by probiotic 42 43 44 49 addition. 45 46 50 4. In conclusion, the addition of probiotic strain Enterococcus faecium M 74 to the feed of 47 48 51 ISA Brown hens reduced cholesterol, lipids, calcium, leucocyte counts and haematocrit values 49 50 51 52 in blood plasma in at least two sampling times, while erythrocyte counts were increased. No 52 53 53 significant effects of probiotic on triglyceride concentration and egg production parameters 54 55 54 were observed. 56 57 58 55 INTRODUCTION 59 60 56 The European Union has banned the use of antibiotic feed additives due to the negative effect E-mail: [email protected] URL: http://mc.manuscriptcentral.com/cbps Page 3 of 20 British Poultry Science 3 1 2 3 57 on human health (Immerseel et al. , 2004); antibiotic resistance is among the greatest threats to 4 5 6 58 public health (Finch and Sharland, 2009). Probiotics show promising effects as alternatives to 7 8 59 antibiotics as pressure to eliminate growthpromoting antibiotic use increases (Awad et al. , 9 10 60 2009). 11 12 13 61 The use of probiotic cultures in the poultry industry has recently become more common 14 15 62 (Torres-Rodriguez et al. , 2007). Probiotics are available as single microbial strains ( e.g ., 16 For Peer Review Only 17 18 63 Bacillus clausii, Lactobacillus ) or as a mix of multiple strains of Lactobacillus ( acidophilus, 19 20 64 sporogenes, lactis, reuteri, plantarum), Bifidobacterium (bifidum , longum, infantis ), 21 22 65 Streptococcus (thermophillus, lactis, fecalis), Saccharomyces boulardii etc. (Singhi and 23 24 25 66 Baranwal, 2008). They have potential health benefits for conditions such as gastrointestinal 26 27 67 infections, genitourinary infections, allergies and certain bowel disorders, all of which afflict 28 29 68 a considerable proportion of the global population (Senok et al. , 2005). Research over the last 30 31 32 69 century has shown that lactic acid bacteria and certain other microorganisms can increase 33 34 70 resistance to disease and that lactic acid bacteria can be enriched in the intestinal tract by 35 36 71 feeding specific carbohydrates (Patterson and Burkholder, 2003). 37 38 39 72 A single dose of Lactobacillus administered intragastrically improved weight gain and 40 41 73 food conversion efficiency (Khan et al ., 2007; Liu et al ., 2007; Zulkifli et al ., 2000), had a 42 43 44 74 growthpromoting effect in broiler chickens (Cavazzoni et al. , 1998) and modulating effects 45 46 75 on immune system of layer- and meat-type chickens (Koenen et al. , 2004) . The application of 47 48 76 Rhodobacter capsulatus into diet may be a feasible way to reduce cholesterol concentration 49 50 51 77 and improve the ratio of unsaturated fatty acids to saturated fatty acids in broiler meat (Salma 52 53 78 et al. , 2007 a). After Bacillus subtilis treatment, egg production, feed consumption and feed 54 55 79 conversion efficiency in layers (Li et al. , 2006) and growth performance of goslings (Wu et 56 57 58 80 al., 2008) was improved. 59 60 81 Probiotic beneficial action in human and animals was also confirmed by Balevi et al. E-mail: [email protected] URL: http://mc.manuscriptcentral.com/cbps British Poultry Science Page 4 of 20 4 1 2 3 82 (2001), Juntunen et al. (2001), Wielen et al. (2002), Glück and Gebbers (2003), Weese and 4 5 6 83 Arroyo (2003), Gil de los Santos et al. (2005), Capcarova et al. (2008), Siggers et al. (2008), 7 8 84 Al-Salami et al. (2008) and others. 9 10 85 Based on the data from literature and the trends of research in the field of probiotics, the 11 12 13 86 aim of the present study was to evaluate the functional efficiency of a probiotic strain 14 15 87 Enterococcus faecium M 74 in the feed on selected biochemical, haematological and 16 For Peer Review Only 17 18 88 production parameters of laying hens. 19 20 89 MATERIAL AND METHODS 21 22 90 Birds, management and diets 23 24 25 91 Forty ISA Brown laying hens were housed at 20 weeks of age in individual cages with a 26 27 92 space allowance of 573 cm2 and given diet KKZ HYD 10 (Table 1). Feed and water were 28 29 93 provided ad libitum . Twenty birds acted as controls and 20 were given the experimental diet. 30 31 32 94 Blood collections were made at 5, 25 and 45 weeks of a 48week laying period. The 33 34 95 experimental group of hens received a probiotic preparation with concentration of 5x10 9 35 36 96 living organisms of Enterococcus faecium M 74 in 1 g. Probiotic preparation (Probiotics 37 38 39 97 International Ltd., UK) was in powder form for ease of incorporation into the diet, and added 40 41 98 to the feed at the rate of 0.5 g/1 kg to produce colony forming units (CFU) of 2.5x10 9 in 1 kg 42 43 Table 1 near here 99 of finished feed.
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