Downloaded from https://academic.oup.com/treephys/advance-article-abstract/doi/10.1093/treephys/tpy083/5063201 by INRA (Institut National de la Recherche Agronomique) user on 16 October 2018 Tree Physiology 00, 1–13 doi:10.1093/treephys/tpy083 Methods paper Mitigating the open vessel artefact in centrifuge-based measurement of embolism resistance Rosana López 1,2,5, Markus Nolf3, Remko A. Duursma3, Eric Badel1, Richard J. Flavel4, Hervé Cochard1 and Brendan Choat3 1Université Clermont Auvergne, INRA, PIAF, 5, chemin de Beaulieu, 63000 Clermont-Ferrand, France; 2Sistemas y Recursos Naturales, Universidad Politécnica de Madrid, C/ José Antonio Novais 10, 28040 Madrid, Spain; 3Hawkesbury Institute for the Environment, Western Sydney University, Hawkesbury Campus, Locked Bag 1797, 2751 Penrith, NSW, Australia; 4School of Environmental and Rural Science, University of New England, Elm Avenue, 2351 Armidale, NSW, Australia; 5Corresponding author (
[email protected]) orcid.org/0000-0003-3553-9148 Received May 4, 2018; accepted July 3, 2018; handling Editor Roberto Tognetti Centrifuge-based techniques to assess xylem vulnerability to embolism are increasingly being used, although we are yet to reach a consensus on the nature and extent of artefactual embolism observed in some angiosperm species. In particular, there is disagree- ment over whether these artefacts influence both the spin (Cavitron) and static versions of the centrifuge technique equally. We tested two methods for inducing embolism: bench dehydration and centrifugation. We used three methods to measure the result- ing loss of conductivity: gravimetric flow measured in bench-dehydrated and centrifuged samples (static centrifuge), in situ flow measured under tension during spinning in the centrifuge (Cavitron) and direct imaging using X-ray computed microtomography (microCT) observations in stems of two species of Hakea that differ in vessel length.