Accepted Manuscript

Isolation and characterization of monocyte/macrophage from peripheral blood of half smooth tongue sole (Cynoglossus semilaevis)

Zhenxia Sha, Linqing Wang, Luming Sun, Yadong Chen, Yuan Zheng, Mei Xin, Chen Li, Songlin Chen

PII: S1050-4648(17)30222-X DOI: 10.1016/j.fsi.2017.04.015 Reference: YFSIM 4540

To appear in: Fish and Shellfish Immunology

Received Date: 11 November 2016 Revised Date: 12 April 2017 Accepted Date: 18 April 2017

Please cite this article as: Sha Z, Wang L, Sun L, Chen Y, Zheng Y, Xin M, Li C, Chen S, Isolation and characterization of monocyte/macrophage from peripheral blood of half smooth tongue sole (Cynoglossus semilaevis), Fish and Shellfish Immunology (2017), doi: 10.1016/j.fsi.2017.04.015.

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1 Isolation and characterization of monocyte/macrophage from peripheral

2 blood of half smooth tongue sole ( Cynoglossus semilaevis )

3

4 Zhenxia sha 1, 2 *, Linqing Wang 2, 3, Luming Sun 2, Yadong Chen 1, 2 , Yuan Zheng 4, Mei Xin 5, Chen 5 Li 2, 4 , Songlin Chen 2, 4* 6 1 College of Life Sciences, University, Qingdao 266071, 7 2 Function Laboratory for Marine Biology and Biotechnology, Qingdao National Oceanography Laboratory, 8 Qingdao 266235, China 9 3 College of biological science and engineering, University ，Fuzhou 350116 ，China 10 4 Key Lab for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries 11 Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China 12 5 Qingdao Fisheries Technology Extension Station, Qingdao 266071, China

13

14 * Corresponding authors.

15 Ningxia Road 308, Qingdao University, Qingdao 266071 China, Tel.: 0086-532-85953227, email

16 address: [email protected] MANUSCRIPT 17 Road 106, Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries

18 Sciences, Qingdao 266071, email address: [email protected]

19

20 Abstract

21 In the present study, the peripheral blood cells of half smooth tongue sole ( Cynoglossus semilaevis )

22 were examined by blood smear under the light microscopy. The proportion of main types of blood cells 23 are as following:ACCEPTED erythrocyte occupied the majority (92.3%), followed by thrombocyte (4.15%), 24 granulocyte (1.7%), lymphocyte (1.5%) and monocyte (0.3%), respectively. Meanwhile, the isolation

25 method of monocytes was established, by density gradient centrifugation to isolate mononuclear

26 leukocytes of peripheral blood. In primary culture, the monocytes were adhered to the bottom of

27 the flask without feeder cells and separated easily with suspended leukocytes in the medium in 3 Download English Version: https://daneshyari.com/en/article/5540483

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