Xylobolus Brasiliensis Fungal Planet Description Sheets 469

468 Persoonia – Volume 42, 2019

Xylobolus brasiliensis Fungal Planet description sheets 469

Fungal Planet 950 – 19 July 2019 Xylobolus brasiliensis Chikowski, C.R.S. de Lira, Gibertoni & K.H. Larss., sp. nov.

Etymology. Name refers to the country where the fungus was collected. Notes — Morphologically, X. brasiliensis is quite similar to Classiﬁcation — Stereaceae, Russulales, Agaricomycetes. X. frustulatus, but the latter has shorter acanthohyphidia (25–30 Basidiomata perennial, stratiﬁed in several layers, resupinate × 4–5 µm) and basidiospores (4.5–5(–5.5) × 3–3.2(–3.5) µm), to effused reflexed, 1–2 mm thick, corky to woody, separated in rare pseudocystidia and elongated basidia (25–30 × 4–5 µm) small irregular patches (0.6–3 × 2.5–10 mm), slightly rimose. (Hjortstam et al. 1988). Abhymenial surface glabrous, dark brown (cigar brown 16). Based on a BLASTn search of NCBIs GenBank database, the Context and margin concolorous with the abhymenial surface. closest hits using the ITS sequence are X. subpileatus (Gen- Hymenial surface greyish brown (Clay buff 32) (Watling 1969), Bank KX578084; Identities = 559/634 (88 %), 27 gaps (4 %)), glabrous, smooth to slightly pilose. Hyphal system monomitic to X. subpileatus (GenBank KX578082; Identities = 558/633 pseudodimitic due to the acanthohyphidia, vertically arranged, (88 %), 27 gaps (4 %)) and X. subpileatus (GenBank KX578080; hyphae clamped. Acanthohyphidia numerous in trama and Identities = 558/634 (88 %), 27 gaps (4 %)). Using the LSU hymenium, cylindrical with obtuse apex, 20–74 × 4–8 µm sequence, the closest hits are Acanthophysium lividocaeru (L = 40.40 µm, W = 6.17 µm, Q = 6.54 µm). Basidia not seen. leum (GenBank AY039314; Identities = 929/947 (98 %), 3 gaps Basidiospores yellowish to brownish, subglobose to ellipsoid, (0 %)), X. subpileatus (GenBank AY039309; Identities = 927/ 5–6(–6.5) × (3–)3.5–5 µm (L = 5.8 µm, W = 3.75 µm, Q = 1.52 947 (98 %), 4 gaps (0 %)) and X. subpileatus (GenBank µm), slightly thick-walled, smooth in KOH 3 %, minutely orna- AY039307; Identities = 927/947 (98 %), 3 gaps (0 %)). mented in Melzer, with a lateral prominent apiculus, distinctly Although genetically close to X. subpileatus, this species differs amyloid. by effused-reflexed basidiomata, tuberculated hymenium when Typus. Brazil, Paraíba, Areia, Reserva Estadual Mata do Pau-Ferro, young, smaller, acute to subcylindrical acanthohyphidia (20–30 S6°59' W35°45', on decaying wood, Apr. 2013, C.R.S. Lira CL 632 (holotype × 4–5 µm) and longer basidia (20–30 × 4–5 µm) (Bernicchia URM 93051, isotype in O, ITS and LSU sequences GenBank MK491193 & Gorjón 2010). and MK491189, MycoBank MB830132).

Additional materials examined. Brazil, Alagoas, Pilar, RPPN Fazenda de São Pedro, on decaying wood, Nov. 2001, T.B. Gibertoni TBG 106, URM 77155; Paraíba, Areia, Reserva Estadual Mata do Pau-Ferro, on decaying wood, Apr. 2013, C.R.S. Lira CL 619, URM 93052; Pernambuco, Jaqueira, Reserva Particular do Patrimônio Natural Frei Caneca, S08°42'41" W35°50'30", on decaying wood, June 2012, R.S. Chikowski RC 71, URM 85814; ibid., Mar. 2013, R.S. Chikowski RC 552, URM 85815; ibid., Mar. 2013, R.S. Chikowski, RC 553, URM 85818; ibid, Apr. 2013, R.S. Chikowski RC 659, URM85817.

Phylogenetic reconstruction of Stereaceae based on alignment of 1 593 nucleotides of combined ITS and LSU rDNA sequences. Bootstrap values Colour illustrations. Environment where the type specimen was collected, (%) were generated from Maximum Likelihood (ML) analysis, and posterior Reserva Estadual Mata do Pau-Ferro, Areia, Paraíba, Brazil. Dried basidi- probabilities (PP) from Bayesian algorithm (BA), respectively. Species in bold oma (type specimen); basidiospores; acanthohyphidia. Scale bars = 1 mm were sequenced in this study. Gloeodontia discolor (GenBank AF506445) (basidioma), 5 µm (basidiospores), 30 µm (acanthohyphidia). and G. pyramidata (GenBank AF506446) were selected as outgroup.

Renata S. Chikowski, Carla R.S. Lira & Tatiana B. Gibertoni, Departamento de Micologia, Universidade Federal de Pernambuco, Recife, Brazil; [email protected], [email protected] & [email protected] Karl-Henrik Larsson, Natural History Museum, P.O. Box 1172 Blindern 0318, University of Oslo, Norway; e-mail: [email protected]