KHDRBS1/Sam68 Rabbit Mab

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KHDRBS1/Sam68 Rabbit mAb

Catalog No.: A3886 Recombinant

Basic Information Background

Catalog No. This gene encodes a member of the K homology domain-containing, RNA-binding, A3886 signal transduction-associated protein family. The encoded protein appears to have many functions and may be involved in a variety of cellular processes, including Observed MW alternative splicing, cell cycle regulation, RNA 3'-end formation, tumorigenesis, and 68kDa regulation of human immunodeficiency virus gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012] Calculated MW 68 kDa

Category Primary antibody

Applications WB, IHC, IF

Cross-Reactivity Human, Mouse, Rat

Recommended Dilutions Immunogen Information

WB 1:500 - 1:2000 Gene ID Swiss Prot 10657 Q07666 IHC 1:50 - 1:200 Immunogen 1:50 - 1:200 IF A synthesized peptide derived from human KHDRBS1/KHDRBS1/Sam68

Synonyms Sam68; p62; p68

Contact Product Information

www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification

Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. Validation Data

Western blot analysis of extracts of various cell lines, using KHDRBS1/KHDRBS1/Sam68 Rabbit mAb (A3886) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- embedded rat brain using embedded human colon using embedded mouse brain using KHDRBS1/KHDRBS1/Sam68 Rabbit mAb KHDRBS1/KHDRBS1/Sam68 Rabbit mAb KHDRBS1/KHDRBS1/Sam68 Rabbit mAb (A3886) at dilution of 1:100 (40x lens). (A3886) at dilution of 1:100 (40x lens). (A3886) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells Immunofluorescence analysis of NIH-3T3 Immunofluorescence analysis of U-2 OS using KHDRBS1/KHDRBS1/Sam68 Rabbit cells using KHDRBS1/KHDRBS1/Sam68 cells using KHDRBS1/KHDRBS1/Sam68 mAb (A3886) at dilution of 1:100 (40x Rabbit mAb (A3886) at dilution of 1:100 Rabbit mAb (A3886) at dilution of 1:100 lens). Blue: DAPI for nuclear staining. (40x lens). Blue: DAPI for nuclear staining. (40x lens). Blue: DAPI for nuclear staining.