Catalog # Aliquot Size

P58-911B-05 3 x 5 nmol P58-911B-20 3 x 20 nmol P58-911B-50 3 x 50 nmol

PTPRM siRNA Set I siRNA duplexes targeted against three exon regions

Catalog # P58-911B

Lot # Z2085-32

Specificity Formulation PTPRM siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human PTPRM expression. shipped at room temperature.

Product Description Reconstitution Protocol PTPRM siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human PTPRM mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The Resuspend the siRNA in 50 µl of DEPC-treated water accession number is BC051651. (supplied by researcher), which results in a 1x stock

Gene Aliases solution (10 µM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot RPTPM, RPTPU, PTPRL1, hR-PTPu, R-PTP-MU 1x stock solutions for storage. Storage and Stability The lyophilized powder is stable for at least 4 weeks at Related Products room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or Product Name Catalog Number below -20oC. After resuspension, siRNA stock solutions ≥2 PTPRA (LRP), Active P48-21G µM can undergo up to 50 freeze-thaw cycles without PTPRC (CD45), Active P50-21H significant degradation. For long-term storage, it is PTPRC (CD45), Active P50-21BG recommended that the siRNA is stored at -70oC. For most PTPRE, Active P52-21G favorable performance, avoid repeated handling and PTPRF (LAR), Active P53-21G multiple freeze/thaw cycles. PTPRS(PTP-sigma), Active P64-21G PTPRM (PTP-mu) Protein P58-35G Scientific Background PTPRM is a member of the protein tyrosine phosphatase family and can participate in a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation (1). PTPRM has been shown to mediate cell-cell aggregation through PTPRM siRNA Set I the interaction with another molecule of PTPRM on an siRNA duplexes targeted against three exon regions adjacent cell. PTPRM can interact with scaffolding Catalog # P58-911B protein RACK1/GNB2L1 and this interaction may be Lot # Z2085-32 necessary for downstream signaling in response to cell- cell adhesion (2). PTPRM has been shown to be expressed Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol Format Lyophilized powder in human pulmonary vascular endothelia where it directly Stability 1yr at -70oC from date of shipment binds to VE- and regulates both the tyrosine Storage & Shipping The lyophilized powder is stable for at phosphorylation state of VE-cadherin and barrier least 4 weeks at room temperature. It is integrity. recommended that the lyophilized and resuspended siRNAs are stored at

or below -20oC. After resuspension, References siRNA stock solutions ≥2 µ M can 1. Phillips-Mason, P.J. et al: The receptor protein-tyrosine undergo up to 50 freeze-thaw cycles phosphatase PTPmu interacts with IQGAP1. J. Biol. without significant degradation. For long-term storage, it is recommended Chem. 2006; 281(8):4903-10. that the siRNA is stored at -70oC. For 2. Koop, E.A. et al: Receptor protein tyrosine most favorable performance, avoid phosphatase mu expession as a marker for repeated handling and multiple freeze/thaw cycles. endothelial cell heterogeneity; analysis of RPTPmu gene expression using LacZ knock-in mice. Int. J. Dev. Biol. 2003; 47(5):345-54

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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.