Sherlock Q-FAMETM

The New Standard for Aerobic Bacterial Identification

Q-FAME Kit Benefits Sherlock Q-FAMETM Sample Preparation Method

Optimized for Clinical Q-FAME is a new rapid microbial identification kit for the Sherlock® Laboratories Microbial Identification System (MIS), which has been optimized for clinical laboratories needing STAT processing of cultures. Q-FAME involves a novel fatty acid-based chemistry that takes less then 25 minutes to identify aerobic bacteria from pure culture. The Q-FAME Kit Contains all Items to Startup Kit includes all necessary items (except growth media) to Prepare a Sample prepare samples, including one refill kit for 192 samples. Q-FAME contains two aerobic bacteria libraries: one for clinical isolates and one for environmental isolates. Refill kits are available in one universal size Culture to ID in Less of 192 samples. Than 25 Minutes The Sherlock® Microbial Identification System (MIS)

Since 1991, over 750 clinical and environmental laboratories Standard Growth ® Media Used have used the Sherlock MIS for rapid microbial identification. Sherlock® MIS is fully automated and capable of identifying aerobic bacteria, anaerobic bacteria, mycobacteria and yeast by gas chromatographic analysis of fatty acid methyl esters (GC-FAME). Over 425 Aerobes Identified in One Test Sherlock® MIS uses sophisticated pattern recognition software to compare unknown FAME profiles to stored “known” profiles to perform each identification. Optional tools allow for strain tracking, custom library generation and 21CFR Part 11 compliance.

No GC experience is required to operate the Sherlock® MIS.

Q-FAMETM Rapid Microbial ID Method

For research use only. Not for use in diagnostic procedures. Q-FAME BENEFITS

Q-FAME vs. Competing Phenotypic-Based Microbial ID Methods

Rapid turnaround time - Q-FAME takes less then 25 minutes to identify an aerobic bacterial culture, at least 400% faster than competing phenotypic-based ID methods.

Reduced growing time required - Q-FAME can often be used after 24 hours of growth from the primary isolation plate, versus phenotypic-based ID methods which require subculturing (24 hours extra growth).

Fewer bacterial cells required - Q-FAME uses 7-8 mg of cells, at least 20% fewer cells than competing phenotypic-based ID methods.

Lower cost per sample - Q-FAME costs less than $7.00 USD per test in consumables, at least 30% less expensive per test than competing phenotypic-based ID methods.

Q-FAME vs. Traditional MIDI Sherlock MIS Sample Preparation

Reduced sample preparation time - Q-FAME involves a novel fatty acid-based chemistry that reduces the sample preparation time from 90 minutes to 15 minutes.

Less waste produced - Q-FAME generates less than 1ml of waste per sample.

More environmentally friendly - Q-FAME reagents do not include MTBE, a ground water and soil contaminant.

No more waterbaths used - Q-FAME uses dry heat, so no more water baths are required.

No more TSBA media used for Environmental Aerobes - Q-FAME uses TSA at 30C instead of TSBA at 28C as the growth conditions for environmental aerobic bacteria (QTSA1 Library). Q-FAME SPECIFICATIONS

Q-FAME Start Up Kit Contents (120V & 240V available)

 Clinical Aerobe Library (QBA1)  Environmental Aerobe Library (QTSA1)  1 mini vortexer*  1 mini centrifuge  1 dry block heater  1 aluminum heat block  3 liquid dispensers  1 reference pipette  1 pipette tip box  1 right angle thermometer  2 vial rack trays  1 custom wooden stand  1 Q-FAME Refill Kit

* Optional digital vortexer (recommended for high sample volume work)

Q-FAME Refill Kit Contents – for 192 Samples

 Glass conical inserts  Clear 2ml GC vials  PTFE/Silicon/PTFE caps  3 Q-FAME extraction reagents  Hexane wash reagent  1ul sterile inoculation loops  Pipette tips  1 MIDI GC injection port liner  2 ampoules of MIDI Rapid Calibration Standard

Typical Customer Required Items*  Sherlock® Microbial Identification System version 6.0 or higher  Agilent 6850, 6890 or 7890 model GCs, configured to MIDI specifications  35C Incubator, BBL Brand TSA with 5% defibrinated sheep blood & Chocolate Agar (Clinical Aerobes – QBA1 Library)  30C Incubator & BBL Brand TSA (Environmental Aerobes – QTSA1 Library)  Isopropanol and methanol (ACS Grade)  Pasteur pipettes, 5 inch, disposable  Chemical storage cabinets (for corrosives and flammables)  Hazardous chemical waste disposal container  Hazardous medical waste disposal container  Chemical fume hood (depending on customer’s requirements)

* See Q-FAME Operating Manual for a complete list of required items Sherlock Q-FAME Libraries

Genus-species QBA1 QTSA1 Genus-species QBA1 QTSA1 Achromobacter-xylosoxidans   Bacillus-mycoides   Acidovorax-avenae   Bacillus-pumilus   Acidovorax-delafieldii   Bacillus-simplex   Acidovorax-facilis   Bacillus-subtilis   Acinetobacter-baumannii   Bacillus-thuringiensis   Acinetobacter-calcoaceticus   Bartonella-henselae  Acinetobacter-haemolyticus   Bibersteinia-trehalosi  Acinetobacter-johnsonii   Bordetella-avium  Acinetobacter-lwoffii   Bordetella-bronchiseptica  Acinetobacter-radioresistens   Bordetella-holmesii  Actinobacillus-lignieresii  Bordetella-parapertussis  Actinobacillus-ureae  Bordetella-pertussis  Aeromonas-hydrophila   Bordetella-trematum  Aeromonas-salmonicida   Brevibacillus-agri   Aeromonas-schubertii  Brevibacillus-borstelensis  Aeromonas-sobria   Brevibacillus-brevis   Aeromonas-trota  Brevibacillus-choshinensis   Aeromonas-veronii  Brevibacillus-laterosporus   Aggregatibacter-aphrophilus  Brevibacillus-parabrevis  Aggregatibacter-segnis  Brevibacterium-casei   Alcaligenes-faecalis   Brevibacterium-epidermidis   Amycolatopsis-orientalis   Brevibacterium-iodinum   Aneurinibacillus-aneurinilyticus  Brevibacterium-linens   Arcanobacterium-haemolyticum  Brevundimonas-diminuta   Arcanobacterium-pyogenes  Brevundimonas-vesicularis   Arthrobacter-aurescens   Burkholderia-anthina   Arthrobacter-crystallopoietes   Burkholderia-caryophylli   Arthrobacter-globiformis   Burkholderia-cenocepacia   Arthrobacter-pascens  Burkholderia-cepacia   Avibacterium-paragallinarum  Burkholderia-cepacia complex  Bacillus-amyloliquefaciens   Burkholderia-gladioli   Bacillus-atrophaeus   Burkholderia-multivorans   Bacillus-badius  Burkholderia-pyrrocinia   Bacillus-cereus   Campylobacter-coli  Bacillus-circulans   Campylobacter-fetus  Bacillus-coagulans   Campylobacter-jejuni  Bacillus-firmus   Cardiobacterium-hominis  Bacillus-freudenreichii"   Cedecea-davisae   Bacillus-gibsonii   Cedecea-lapagei  Bacillus-lentus   Cellulomonas-fimi   Bacillus-licheniformis   Cellulosimicrobium-cellulans   Bacillus-megaterium   Chromobacterium-violaceum   Sherlock Q-FAME Libraries

Genus-species QBA1 QTSA1 Genus-species QBA1 QTSA1 Chryseobacterium-balustinum   Enterobacter-cancerogenus   Chryseobacterium-gleum   Enterobacter-cloacae   Chryseobacterium-indologenes   Enterobacter-gergoviae  Citrobacter-farmeri  Enterobacter-hormaechei   Citrobacter-freundii   Enterococcus-avium  Citrobacter-koseri   Enterococcus-casseliflavus   Clavibacter-michiganensis   Enterococcus-cecorum  Comamonas-terrigena   Enterococcus-columbae  Comamonas-testosteroni   Enterococcus-dispar   Corynebacterium-afermentans   Enterococcus-durans   Corynebacterium-amycolatum  Enterococcus-faecalis   Corynebacterium-auris   Enterococcus-faecium   Corynebacterium-bovis  Enterococcus-gallinarum   Corynebacterium-coyleae   Enterococcus-hirae   Corynebacterium-diphtheriae  Enterococcus-malodoratus  Corynebacterium-flavescens   Enterococcus-mundtii  Corynebacterium-jeikeium  Enterococcus-raffinosus  Corynebacterium-kutscheri   Escherichia-coli   Corynebacterium-minutissimum   Escherichia-fergusonii   Corynebacterium- Exiguobacterium-acetylicum   pseudodiphtheriticum   Flavobacterium-johnsoniae   Corynebacterium- pseudotuberculosis   Flavobacterium-mizutaii   Corynebacterium-renale   Fluoribacter-bozemanae  Corynebacterium-striatum   Fluoribacter-dumoffii  Corynebacterium-urealyticum  Fluoribacter-gormanii  Corynebacterium-xerosis   Francisella-philomiragia  Cronobacter-sakazakii   Gardnerella-vaginalis  Curtobacterium-flaccumfaciens   Geobacillus-stearothermophilus   Curtobacterium-luteum   Geobacillus-thermoglucosidasius   Curtobacterium-pusillum   Gordonia-aichiensis   Deinococcus-radiodurans   Gordonia-amarae  Delftia-acidovorans   Gordonia-bronchialis   Dermabacter-hominis   Gordonia-rubripertincta   Dietzia-maris   Gordonia-sputi   Edwardsiella-hoshinae  Gordonia-terrae   Edwardsiella-tarda   Haemophilus-influenzae  Eikenella-corrodens  Haemophilus-parahaemolyticus  Elizabethkingia-meningoseptica   Haemophilus-parainfluenzae  Empedobacter-brevis  Hafnia-alvei  Enterobacter-aerogenes   Helicobacter-pylori  Enterobacter-amnigenus  Hydrogenophaga-pseudoflava   Enterobacter-asburiae  Jonesia-denitrificans  Sherlock Q-FAME Libraries

Genus-species QBA1 QTSA1 Genus-species QBA1 QTSA1 Kingella-denitrificans  Microbacterium-aurum   Kingella-kingae  Microbacterium-barkeri   Klebsiella-oxytoca   Microbacterium-chocolatum   Klebsiella-pneumoniae   Microbacterium-dextranolyticum   Kluyvera-ascorbata  Microbacterium-esteraromaticum   Kocuria-kristinae   Microbacterium-flavescens   Kocuria-rhizophila   Microbacterium-hominis   Kocuria-rosea   Microbacterium- Kocuria-varians   hydrocarbonoxydans   Kytococcus-sedentarius   Microbacterium-imperiale   Lactobacillus-delbrueckii   Microbacterium-keratanolyticum   Lactobacillus-pentosus  Microbacterium-lacticum   Lactobacillus-plantarum   Microbacterium-laevaniformans   Lactobacillus-reuteri   Microbacterium-liquefaciens   Lactobacillus-rhamnosus  Microbacterium-luteolum   Lactococcus-garvieae  Microbacterium-maritypicum   Lactococcus-lactis   Microbacterium-saperdae   Lactococcus-plantarum  Microbacterium-schleiferi   Leclercia-adecarboxylata   Microbacterium-terrae   Legionella-geestiana  Microbacterium-testaceum   Legionella-jordanis  Micrococcus-luteus   Legionella-pneumophila  Micrococcus-lylae   Legionella-sainthelensi  MIDI-Calibration   Leminorella-grimontii  Moellerella-wisconsensis  Leuconostoc-mesenteroides  Moraxella-atlantae  Leuconostoc- Moraxella-bovis  pseudomesenteroides  Moraxella-canis  Listeria-grayi  Moraxella-catarrhalis  Listeria-innocua   Moraxella-nonliquefaciens  Listeria-ivanovii  Moraxella-osloensis  Listeria-monocytogenes   Morganella-morganii   Listeria-seeligeri  Mycobacterium-abscessus   Lysinibacillus-sphaericus   Mycobacterium-agri  Macrococcus-caseolyticus   Mycobacterium-aurum  Methylobacterium-aminovorans   Mycobacterium-brumae  Methylobacterium-extorquens   Mycobacterium-chelonae  Methylobacterium-fujisawaense   Mycobacterium-chitae  Methylobacterium-mesophilicum   Mycobacterium-chlorophenolicum  Methylobacterium-organophilum   Mycobacterium-chubuense  Methylobacterium-radiotolerans   Mycobacterium-diernhoferi  Methylobacterium-rhodesianum   Mycobacterium-fallax  Methylobacterium-zatmanii   Mycobacterium-flavescens  Microbacterium-aurantiacum   Mycobacterium-fortuitum   Sherlock Q-FAME Libraries

Genus-species QBA1 QTSA1 Genus-species QBA1 QTSA1 Mycobacterium-komossense  Paenibacillus-peoriae  Mycobacterium-moriokaense  Paenibacillus-polymyxa   Mycobacterium-mucogenicum   Paenibacillus-thiaminolyticus   Mycobacterium-neoaurum  Paenibacillus-validus   Mycobacterium-obuense  Pandoraea-apista   Mycobacterium-smegmatis   Pantoea-agglomerans   Mycobacterium-sphagni  Paracoccus-denitrificans   Mycobacterium-vaccae  Pasteurella-multocida  Myroides-odoratus   Pectobacterium-carotovorum   Neisseria-cinerea  Plesiomonas-shigelloides  Neisseria-elongata  Proteus-mirabilis   Neisseria-flava  Proteus-penneri  Neisseria-flavescens  Proteus-vulgaris   Neisseria-gonorrhoeae  Providencia-alcalifaciens   Neisseria-meningitidis  Providencia-rettgeri  Neisseria-mucosa  Providencia-rustigianii  Neisseria-sicca   Pseudomonas-aeruginosa   Neisseria-subflava  Pseudomonas-alcaligenes  Neisseria-weaveri  Pseudomonas-chlororaphis   Nocardia-asteroides   Pseudomonas-fluorescens   Nocardia-brasiliensis   Pseudomonas-luteola   Nocardia-brevicatena  Pseudomonas-mendocina   Nocardia-farcinica   Pseudomonas-oryzihabitans   Nocardia-globerula   Pseudomonas-pseudoalcaligenes   Nocardia-nova   Pseudomonas-putida   Nocardia-otitidiscaviarum   Pseudomonas-savastanoi   Nocardia-pseudobrasiliensis  Pseudomonas-stutzeri   Nocardioides-albus  Pseudomonas-syringae   Novosphingobium- Psychrobacter-phenylpyruvicus  aromaticivorans   Ralstonia-eutropha   Novosphingobium-capsulatum   Ralstonia-pickettii   Novosphingobium-subterraneum   Ralstonia-solanacearum   Ochrobactrum-anthropi   Rhizobium-radiobacter   Oerskovia-turbata   Rhodococcus-coprophilus  Oligella-ureolytica  Rhodococcus-equi   Oligella-urethralis  Rhodococcus-erythropolis   Paenibacillus-alvei  Rhodococcus-rhodochrous   Paenibacillus-amylolyticus  Riemerella-anatipestifer  Paenibacillus-azotofixans  Roseomonas-cervicalis   Paenibacillus-glucanolyticus   Roseomonas-fauriae   Paenibacillus-lautus   Roseomonas-gilardii  Paenibacillus-macerans   Salmonella-enterica   Paenibacillus-pabuli   Sherlock Q-FAME Libraries

Genus-species QBA1 QTSA1 Genus-species QBA1 QTSA1 Serratia-liquefaciens   Staphylococcus-sciuri   Serratia-marcescens   Staphylococcus-simulans   Serratia-odorifera   Staphylococcus-warneri   Serratia-rubidaea  Staphylococcus-xylosus   Shewanella-putrefaciens   Stenotrophomonas-maltophilia   Shigella-boydii  Streptococcus-agalactiae  Shigella-dysenteriae  Streptococcus-bovis   Shigella-flexneri  Streptococcus-canis  Shigella-sonnei   Streptococcus-cristatus  Sphingobacterium-multivorum   Streptococcus-dysgalactiae  Sphingobacterium-spiritivorum   Streptococcus-equi  Sphingomonas-adhaesiva   Streptococcus-equinus  Sphingomonas-macrogoltabidus   Streptococcus-gallolyticus  Sphingomonas-parapaucimobilis   Streptococcus-gordonii  Sphingomonas-paucimobilis   Streptococcus-infantarius  Sphingomonas-terrae   Streptococcus-infantis  Sphingomonas-yanoikuyae   Streptococcus-iniae  Staphylococcus-arlettae  Streptococcus-intermedius  Staphylococcus-aureus   Streptococcus-mitis  Staphylococcus-auricularis   Streptococcus-mutans  Staphylococcus-capitis   Streptococcus-oralis  Staphylococcus-caprae   Streptococcus-parasanguinis  Staphylococcus-carnosus  Streptococcus-pneumoniae  Staphylococcus-chromogenes  Streptococcus-pyogenes  Staphylococcus-cohnii  Streptococcus-salivarius  Staphylococcus-delphini  Streptococcus-sanguinis  Staphylococcus-epidermidis   Streptococcus-suis  Staphylococcus-equorum  Suttonella-indologenes  Staphylococcus-felis  Tatlockia-maceachernii  Staphylococcus-gallinarum  Tatlockia-micdadei  Staphylococcus-haemolyticus   Tsukamurella-paurometabola   Staphylococcus-hominis   Vibrio-alginolyticus  Staphylococcus-hyicus  Vibrio-cholerae  Staphylococcus-intermedius  Vibrio-mimicus   Staphylococcus-kloosii  Vibrio-parahaemolyticus   Staphylococcus-lentus  Vibrio-vulnificus   Staphylococcus-lugdunensis   Virgibacillus-pantothenticus   Staphylococcus-lutrae  Weeksella-virosa   Staphylococcus-muscae  Weissella-confusa   Staphylococcus-pasteuri  Xanthobacter-agilis   Staphylococcus-saprophyticus   Xanthobacter-flavus   Staphylococcus-schleiferi   Xanthomonas-axonopodis   Sherlock Q-FAME Libraries

Genus-species QBA1 QTSA1 Xanthomonas-campestris   Xanthomonas-hortorum   Yersinia-enterocolitica   Yersinia-frederiksenii   Yersinia-intermedia   Yersinia-kristensenii   Yersinia-pseudotuberculosis  Yersinia-ruckeri 

The information in this publication is subject to change without notice. Copyright  2010 MIDI, Inc. All Rights Reserved Date: 29 September 2010 MIDI, Inc. 125 Sandy Drive Newark, Delaware 19713 USA Phone: 302-737-4297 Fax: 302-737-7781 Email: [email protected]