GENETIC VARIATION IN BLOOD PROTEINS WITHIN AND DIFFERENTIATION BETWEEN 19 SHEEP BREEDS FROM SOUTHERN AFRICA
By .
JANICE SARGENT
Dissertation
presented in partial fulfillment .of
the requirements for the degree
MASTER of SCIENCE
ZOOLOGY
in the
FACULTY 'OF-NATURAL SCIENCES
of the
Rand . Afrikaans University
Supervisor: Prof F.H. van der Bank
Co-suPervisor: Antoinette Kotze
October 2000 . PAGE LIST OF CONTENTS
ABSTRACT ii
OPSOMMING iii
PREFACE vi
ACKNOWLEDGMENTS
CHAPTER 1 1 - 1 1. INTRODUCTION 1 - 2 2. COMPARITIVE CHARACTERISTICS OF 19 SHEEP 1 - 5 BREEDS FROM SOUTHERN AFRICA 3. OBJECTIVES OF STUDY 1 - 42
CHAPTER 2 2 - 1 GENETIC VARIATION IN BLOOD PROTEIENS 2 - 1 WITHIN AND BETWEEN 19 SHEEP BREEDS FROM SOUTHERN AFRICA MATERIALS AND METHODS 2 - 2 RESULTS 2-12 DISCUSSION 2-22
CHAPTER 3 SUMMARY 3 - 1
CHAPTER 4 4 - 1 REFERENCES 4 - 2
APPENDIX I GENEPOP INPUT FILE FOR 19 SHEEP BREEDS GENEPOP INPUT FILE FOR SIX SHEEP BREEDS
APPENDIX II ELECTROPHORETIC RESULTS OF SELECTED ENZYME SYSTEMS GELS 1. PEROXIDASE GLUCOSE-6-PHOSPHATE ISOMERASE HAEMOGLOBIN LACTOSE DEHYDROGENASE Abstract
The amount of allozyme variation within, and the extent of genetic differentiation between, 19 sheep breeds from southern Africa were determined by six enzyme coding loci. Another eight enzyme coding loci were analyzed for five breeds. Between 55 and 66.67% of the protein coding loci were polymorphic (95% criterion) in all the breeds, except for the Namaqua sheep that were less polymorphic (33.33%). Values of 1.67 to 2.5 were obtained for the mean number of alleles per locus and average heterozygosities per locus was between 16.6 to 35.9%. The allelic constitution particularly at the transferrin (TF) locus varied appreciably for the different breeds. For example, the TF*H allele was exclusively noted in the Dormer sheep and the TF*G allele was found in the Afiino, Van Rooy, Border Leicester, Blackhead Persian and Skilder-Persian breeds. The only polymorphic breeds at the albumin locus were the South African Mutton Merino and Van Rooy breeds. The allelic constitution at the other polymorphic loci was similar for the breeds, but the allele frequencies of the South African Merino differs from Merino breeds in other countries at the TF locus. Unbiased genetic distance values were the smallest between the Dorper and Dormer breeds and the largest between the Romenof and Blackhead Persian breeds, and the mean genetic distance between the 19 breeds was 0.067. The mean amount of differentiation among the breeds relative to ' the limiting amount under complete fixation (F ST) was calculated at 0.123, which is an indication of small genetic differentiation between the breeds studied. However, this , statistic is not reflected by the allele distribution that was not identical for 25 breed pairs (15%) of the total (171) at all the genetic blood systems studied. None of the breed pairs showed identical allele distributions at all the loci studied for at least one locus differed at each breed pair compared. Random amplified polimorphic DNA's gave no consistent or repeatable results. The results of the allozyme study presents the first study of the current genetic characterization of the different southern African sheep breeds.
ii OpSomming Die hoeveelheid allosiemvariasie binne en' die mate van genetiese differensiasie tussen 19 skaaprasse van suider Afrika was bepaal deur Van ses algemeen genetiese bloedsisteme gebruik to maak:Daama is nog agt ensiem-kOderende lokusse vir vyf rasse ontleed. Tussen 55 en 66.67% van die proteien-koderende lokusse was polimorfies (95% kriterium) vir al die rasse, behaiwe vir die • Namaqua skaapras wat slegs 33.33% polimorfies was. Die gemiddelde aantal allele per lokus wissel van 1.67 tot 2.5, en die gemiddelde heterosigositeit 'per lokus was 16.6 tot 35.9%. Die alleliese samestelling, veral by die, transferrien (TF) lokus het merkbare. verskille tussen die verskillende rasse getoon. Byvoorbeeld, die TF*H alleel was slegs by die Dormer skaapras gevind en die TF*G alleel in die Afrino, Van Rooy; Borderleichester, Swartkop-persie and Skilder-Persie rasse gevind. Die Suid Afificaanse Vleis Merino en Van RoOy rasse was die enigste polimbrfiese rasse by die albumien. lokus. Die alleliese samestelling by die res van die lokusse was soortgeyk vir al die rasse. Die Suid Afrikaanse Merino verskil by die TF lokus van Merino rasse in ander lande. Die genetiese afstand was die kleinste tussen die Dorper en Dormer rasse en die grOotste tussen die Romenof en Swartkop-persie rasse, met 'n gemiddelde genetiese afstand van 0.067 tussen die 19 rasse. Die gemiddelde hoeveelheid differensiasie tussen die rasse, relatief tot die:beperkte aantal onder algehele fticsasie (Fs r) was bereken as 0.123 vir polimorfiese lokusse, wat riaaiidniding -vari min genetiese differensiasie tussen . die rasse is. Merdie statistieke word egter the gereflekteer by al die lokusse wat bestudeer is the, deurdat 25 raspare (15%) van die totaal (171) the identiese alleel- verspreiding het nie. Geen van die raspare .het dieselfde alleelVerspreiding by al die lokusse getoon the; ten minste een lolcus het by:al die raspare verskillende alleelverspreidings getoon. RAPD'5 het. geen kthistante, herhaalbare resultate geleWer nie. Die resultate van die allosiem studie kan in teelProgranime gebruilc word en dit . is die eerste studie wat die huiding genetiese struktuur van die verskillende suider Afrikaanse skaaprasse aandui. Preface
This study was introduced at a local Departmental colloquium (30/09/1997) and it was presented at a National Conference (Sargent, J., Van der Bank, F.H. and Kotze, A., 1998. Genetiese variasie in die bloedproteiene van 19 skaaprasse van Suid-Afrika.
SA Tydskrif vir Natuurwetenskap en Tegnologie, 17:85), and also at a second departmental colloquium (07/10/1998). The results of this study were published in a national journal (Sargent, J., Van der Bank, F.H. and Kotze, A, 1999. Genetic variation in blood proteins within and between 19 sheep breeds from southern Africa.
South African Journal of Animal Science, 29(3): 245-257). This thesis is the final requirement to obtain a M.Sc. degree. AclulOwledgments My sincere gratitude is extended to the following people who contributed to the execution and completion of this project. Prof. F.H. van der Bank, my supervisor and mentor, for his time, guidance, assistance and constructive criticism throughout the duration of this project. Mrs. Antoinette Kotze, my co-supervisor, from the Animal Improvement Institute in Irene, for her patients and assistance in all aspects regarding the practical application of this study. Also for helping to initiate this study, and collect blood samples. Mrs. M. van der Bank for her comments, guidance and assistance in all respects regarding this study. Sasol who has provided financial funding and without whom this project wouldn't have been possible. All the farmers who supplied blood samples. RAU for giving me a much needed bursary to complete my studies MOST IMPORTANT I praise'the Lord for the privilege to study the complexity of His creation I would like to thank Charnay and Reino, my two children, for their love and patience. Last but not least, I am thanking my mother and farther, Myrtle and Patrick van goner, who always lead by example, even during times of hardship.
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:•,:-. CHAPTER 1
INTRODUCTION
CONTENTS: 1 — 1
Introduction 1 —2
Comparative characteristics and normal production environment of the sheep breeds studied:
2.1. Afrino 1 - 6 2.2. Blackhead Persian 1 — 7 2.3. Border Leicester 10 2.4. Damara 1-12 2.5. Dormer 1-14 2.6. Dorper 1-16 2.7. Karakul 1-18 2.8. South African Landskaap (No information) 2.9. Merino 1 — 21 2.100. Namaqua 1-24 2.11 Pedi 1-26 2.12. Perinoff (No Information) 2.13. Romanov 1 2. 28 2.14. Ronderib Afrikaner 32 2.15. Russian,Red Wooled Persian 34 2.16: Skilder Persian -35 2.17. South African Mutton Merino 36 2.18. Vandor 1-39 2.19. Van Rooy 1-41
The objectives of the study 1 — 42
1 - 1 INTRODUCTION
The archaeological record shows that domestic animals have been present in South Africa for almost 2 000 years. The first to arrive were sheep, goats and cattle, followed a little later by dogs, chickens and probably pigs. The earliest fossil record of sheep dates to about 2 000 years ago but sheep and goats separated about 5 million years ago. The fossil record is poor, probably because the normal habitat is unfavorable for fossil formation.
Sheep first appeared in the Villafranchian and from then on appeared sporadically in the
Pleistocene fossil record. Like many of the mammalian fauna of the ice ages, these were large animals that were later replaced by smaller mouflon-like animals. The animals were brought in by early communities migrating from the central lake area of Africa. They adapted to the environment and were able to thrive on poor grazing. These animals have become the indigenous breeds of southern Africa today. The total number of sheep worldwide was around 1087 million in 1994. There are 33 sheep breeds in southern
Africa, which include seven pure indigenous breeds (Terblanche, 1978). Indigenous breeds are defined as breeds with specific characteristics, etc. adapted to a specific environment..
The definition of a breed is either 1) a homogenous, sub specific group of domestic livestock with definable and identifiable external characteristics that enable it to be
separated by visual appraisal from other similarly defined groups within the same species,
or 2) a hothogenous group for which geographical separation from phenotypically similar groups has led to general acceptance of its separate identity. The Food and Agriculture
Organization of the United Nations (FAO) has adopted these definitions in its program on
animal genetic resources.
1 - 2 Indigenous breeds generally have a higher resistance against diseases, their adaptability to the warmer climate in the summer and extreme cold climate during the winter is better. Therefore, it is beneficial to characterize the indigenous sheep breeds and to use them in breeding programs to improve breeding stocks since a combination of different indigenous sheep breeds could lead to better utilisation of the natural resources, which would be economically viable (Tereblanche, 1978).
The domesticated sheep, Ovis aries, is classified as a member of the family Ovidae of the order Artiodactyla. The artiodactyls are one of the most successful of the mammalian orders, the genus Ovis is a good example of the arbitrary nature of classification at the species level, therefore the number of species, subspecies and races will alwa3is remain contentious.
The mechanisms of evolution that led to the development of, for example, wild
Sheep were mutations and chromosomal rearrangement,. selection, migration, geographical isolation and fixation of diversity by inbreeding and random genetic drift in small populations. Domestication increased the speed or the size of the effects of many factors, e.g. inbreeding, out breeding, genetic drift and added artificial selection and assertive mating to the forces changing gene frequencies. The change to domesticated conditions did not create any new inheritance, but the environmental changes following domestication allowed genetic differences to show themselves more clearly than before and thus to be more readily and accurately selected. Examples of important characteristics that can be mentioned include growth rate, fattening ability and milk yield, in which good feeding
1 - 3 increases differences. The change permitted a greater range of variation to survive through reduced natural selection.
Maintaining genetic variation within a pure breeding population is a complex . problem. A possible solution would be to determine the degree of distribution of the current genetic diversity, because no long-term management or meaningful conservation action can be taken without a complete understanding of the current genetic structure of a species. This is an important consideration since the existence of some of the indigenous sheep gene pools are threatened with exotics. The theoretical basis of this study is that breeds can be defined as populations, which differ from each other in the relative distribution and frequencies of genes (Hasselholt, 1969). These differences in allele frequencies indicate each breed's particular identity (Van Zeveren, 1995).
From the general description of immunological and electrophoretic polymorphisms, it appears that there are three characteristics in common which are of basic importance in view of practical applications, both in animal breeding and in fundamental research: a) the phenotype of an individual with respect to these traits does not change during life, b) it can be identified by objective methods of analysis and, c) it is controlled by simple Mendelian inheritance. For these reasons biochemical blood polymorphism was used to study the variability within populations and the divergence
between the different sheep . breeds. It is also a test based on the possibility of detecting inconsistencies between the genotype of an individual and those of the putative parents
An introduction to the sheep breeds studied follows.
1 - 4 2.COMPARATIVE CHARACTERISTISTICS AND NORMAL PRODUCTION ENVIRONMENT OF THE BREEDS STUDIED
The status, breed type and normal production environment of the sheep breeds studied are summarised in Table 1
Table 1. Sheep breed identification, status, breed type and normal production environment.
Sheep breed Status Breed type Production environment Afrino Adapted synthetic Wool and mutton Semi-Arid, Karoo breed breed veld Blackhead Persian. Indigenous Fat rumped breed Semi-arid, Cape Border Leicester Exotic Long wool breed Savannah Damara Indigenous Fat tailed breed Deserts, Namibia Dormer Adapted synthetic Mutton breed Temperate climate, breed whole South Africa Dorper Adaptive synthetic Mutton breed Arid grassveld, breed whole South Africa Karakul Indigenous Fat tailed breed Deserts, Namibia Landrace Exotic Mutton breed Cape Merino Adapted breed Wool breed Sour grassveld, semi-arid areas Namaqua Afrikaner Indigenous Fat tailed breed Karoo Pedi Indigenous Fat tailed breed NothernProvince Perinoff Adaptive Synthetic Mutton breed Karoo breed Romanov Exotic Mutton breed ARC — Irene Ronderib Afrikaner Indigenous Fat tailed breed Northern Cape Russian . Red Woolled Exotic Fat tailed breed Frankfort, Free Persian State Speckled or Skilder Indigenous Fat rumped breed • Dry areas, East Persian Africa South African Mutton Adapted synthetic Mutton breed High rainfall Merino breed grassveld Vandor Adapted synthetic Fat rumped breed Semi-arid grass to breed Karoo veld Van Rooy Adaptive synthetic Fat tailed breed Arid to semi-arid breed savannah, Cape
1-5 2.1 AFRINO
The Afrino is classified as an adapted synthetic wool and mutton breed. This refers to previous exotic breeds that adapted so well to their environment, that their genetic structure changed. The Afrino is a hardy sheep, which can withstand extreme dry conditions. This is a dual-purpose sheep breed. Its wool is a good export product and reaches high selling prices. An average of 3 kg of wool can be shaven off each individual.
Wool of an average of 21 micron can be produced and the meat of slaughtered lambs reaches a relatively high price at the meat market (Landbouweekblad, October 1997).
Right and above: Afrino rams (Landbouweekblad, October 1997).
1- 6 2.2 BLACKHEAD PERSIAN
The ancestors of the Blackhead Persian arrived in South Africa by chance and are one of
the oldest sheep breeds known. A trade ship trading with Arab, Persia and Africa had this
sheep as a slaughter breed on board when the ship broke down near Port Beaufort, close
to Swellendam on the Cape coast, in 1870. These sheep, one ram and three ewes, were
then purchased and taken to Wellington, where the breed was further developed. The
Blackhead Persian, Redhead Persian and Skilder Persian are therefore considered
indigenous (Campbell, 1995).
This breed is classified as a fat rumped breed and is one of the parent breeds of
the well-known Dorper. The Blackhead Persian is a fat rumped slaughter sheep. It has
low nutritional requirements, strong thick skin, short kempy fleece and a long breeding
season. The Persian shows some resistance against ticks and tick borne diseases and
thrive in the Uitenhage bushveld of the Eastern Cape, Savannah veldt. of the Northern
Cape and even in the Kalahari dunes of the northwest Cape.
A prominent jowl is characteristic of this breed. The body covering consist of
short, white lime like kemp, whereas the head is covered with short, shiny, black hair.
These characters protect the sheep against ultra-violet radiation and skin cancer
(Terblanche, 1978). It's tail stores energy in the form of fat and the first part thereof is
-, attached to the rump and the second part pushes upwards and ends in the tassel that hangs
down.
Adult rams and ewes may weigh 80 kg and 50 kg respectively. They are
extremely fertile and can produce lambs right through the year. A lamb percentage of.
approximately 120 can be produced. This is a hardy, adapted breed, with a relative high
1 - 7 natural resistance against most diseases e.g. blue tongue, Geigeria poisoning
("vermeersiekte"), pulpy kidney and tribulosis ("geeldikkop-siekte"). The Blackhead
Persian can tolerate brackwater with a high mineral content better than most other breeds without developing calcified stones in the urinary system (Campbell, 1995)
Persians produce strong valuable skins known in the trade as "glovers" which are used to produce high quality leather goods. For good quality skin, crossings between the
Persian and Karakul breed is preferred. Karakul farmers use Blackhead Persian ewes to upgrade their breeding stock to ensure that the good characteristics of the breed, such as fertility, unlimited breeding season and hardiness' are used.
The Blackhead Persian is bred in the Northern Cape, in the region of Upington,
Douglas and Griekwastad. A few farmers of the northwest region also prefer to farm with this breed. The Persian breeders association is situated in Douglas.
1 - 8 Right: Blackhead Persian ram (Campbell, 1995)
Above: A Flock of Blackhead Persians (Campbell, 1995)
Below and far right: Blackhead Persian ewes (Campbell, 1995)
1 - 9 2.3. BORDER LEICESTER
The Border Lpicester is an exotic sheep breed from Leicestershire, England. It is a long wool breed, and is responsible for the development and improvement of long wool breeds in England. George and Matthew Culley of Fenton, England found the Border Leicester breed in 1767.
The Border Leicester has a prominent chest with long white wool and a pink skin.
The fleece mass from mature females ranges from 8 to 12 pounds (3.7 - 6.6 kg) with a yield of 65 to 80%. The stable length of the fleece rqnges from five to ten inches (12.5 —
25 cm) with a spinning count of 36 to 48, which is 38.5 to 30.0 microns. Mature rams weigh from 225 to 325 pounds (102 - 147 kg) and ewes weigh between 175 to 275 pOunds (79 - 124 kg). They have big, erect ears and a Roman nose (Microsoft Internet
Explorer, 1997).
Above: Border Leicester rams (Microsoft Internet Explorer, 1997)
1-10 Above: A prize winner Border Leicester ram at the Rand Easter Show in 1996 (Landbouweekblad, March
1997) 2.4. DAMARA
The Damara has adapted to harsh circumstances and can survive where water, grazing and shelter are limited. This is an indigenous breed which can withstand most sheep illnesses, external and internal parasites. However, their resistance against hartwater is less than that of the Pedi breed, for this reason, farmers crossbred the .Damara with the
Pedi breed to improve their resistance against 'hartwater. It can resist extreme changes in weather conditions and walk comfortable for miles on end in search of water and grazing.
The average life mass of adult rams is 60 and of ewes approximately 45 kg. It has a fat, long, relative straight tail with a sharp end. The. Damara is covered with short brown or white hair. During the winter the hair tend to be much thicker than in summer time The ewes are smaller than the, rams and have a feminine appearance. The rams show robust muscle formation and have very strong legs. Their ears, skin and tails are very versatile, they tend to be wide-awake, and have strong teeth. High fertility is one of the best characteristics and the ewes present excellent mothering skills. It could be said that this is one of Namibia's hardiest indigenous sheep breeds, and the ram lambs grow very rapidly, using the maximum grazing available.
This indigenous sheep of Namibia is limited to its homeland in the far north of
Damaraland, and especially in the. Kaokoveld from where they are found right to the
Damara skins are extremely valuable and are used to manufacture first class light
weight leather articles such as gloves and light weight jackets. Damara sheep are often
kept in conjunction with game farming (Campbell, 1995).
1-12 Above and above right: Damara ewes (Campbell, 1995)
Right and below: Damara rams (different colors)
(Landbouweekblad, November 1998)
—
1-13 2.5. DORMER
The Dormer sheep breed originated because there was a need to develop a breed that can adapt to the climate and grazing conditions of a winter-rainfall region and still produce young slaughter lambs of high quality. The Dormer was bred by crossbreeding a Dorset
Horn ram, with good mutton conformation, long breeding season and rapid growth capability and a South African Mutton Merino ewe, with high resistance against the
Muellerius-longworm, excellent fertility, good mothering characteristics and the capability to produce more than one lamb at a time. The breed evolved at the former
Elsenburg College of Agriculture in the Western Cape, and is the result of a. series of extensive slaughter-lamb trials carried out from 1927 to 1937. Its name is an abbreviation of the linkage Dorset-Merino.
The Dormer is a polled, smooth body, white-woolled mutton sheep with an average body mass of 100 and 77 kg for adult rams and ewes respectively. The carcass of today's Dormer lamb is full and broad across the shoulders, back and loins, while the hindquarters are well fleshed. A lamb percentage of 150 is a common occurrence. This breed has a high fertility, long breeding season and the capability to produce multiple siblings. Dormer lambs are rather small at birth with a mass of approximately 4 kg, but grow rapidly. The body of the sheep is covered with rough white wool with a spinning count of 54 to 56, while the head- is open and covered with white calcareous hairs: The resistance against worms, for which it was selected in the development of the breed, is
still an important characteristic of the Dormer in the winter-rainfall areas.
1 -.14 The Dormer is exclusively bred for meat production; the slaughter lambs reach a slaughter mass of approximately 15 kg after three to four months. This breed is spread over most parts of South Africa. (Farmers Weekly, April, 1998 and Terblanche, 1978).
Above: Dormer ewes and lambs grazing (Farmers Weekly, April 1998)
1-15 2.6. DORPER
It became clear during the 1930's that South African mutton consumers were beginning to move away from exclusively fatty meat. There was a need for a local mutton breed that would adapt well to the more and extensive farming regions of the country. The Dorper originated from the successful crossbreeding between a British Dorset Horn ram and a
Blackhead Persian ewe in 1936 by. the Meat Control Board at the Grootfontein College of
Agriculture. The cross not only proved exceptionally hardy and drought resistance, but also yielded an excellent slaughter lamb that could be marketed direct from the veld. By
1950 the new hornless breed was well established and the name Dorper was given. It is
considered as the second most popular sheep , breed in South Africa today, with .the greatest concentration in the Northern Cape.
The Dorper is a big, well-confirmed mutton sheep with a white or black head and a white body. The breed can produce lambs almost any time of the year and attain a lambing percentage of 150 per season in good grazing conditions. It is a hardy breed that produces slaughter lambs throughout the year, because this breed was originally bred for the drier regions. The Dorper lamb achieves the high average weaning mass of 36 kg at three to four months of age. This is the result of the excellent mothering qualities of the ewe and the lamb's ability to start grazing when still quite young.
The Dorper skin is one of the world's most sought-after, and is marketed under the brand name of Cape Glovers. In the past it attained as much as 20% of the total carcass value, but recently all skin prices have shown a considerable decline. The main breeding purpose of this breed is to produce slaughter lambs in extensive veld conditions.
1-16 The Dorper is found throughout South Africa and neighboring countries especially in Zimbabwe (Terblanche, 1978; Farmers Weekly, February 27, 1998)
Above: A flock of Dorper sheep in the. Kalahari
Left: Dorper Ram (Both figures: Farmers Weekly
February 27, 1998)
1-17 2.7 KARAKUL
According to archaeological evidence, it is believed that the Karakul existed some 1 400 years BC. Carvings of a distinct Karakul type have been discovered in ancient
Babylonian Temples, therefore we can state that it is the oldest domesticated sheep breed in the world. The Karakul derived its name from a lake in the Turkestan, Russia, Kara
Kul. However the breed probably originated in Arabia, and spread to the Russian steppes,
Middle East, Germany and many other countries, including South Africa. In 1916 Alan
Tompson, who then developed the first shallow curl and silky water types, brought the first three Karakul rams and 84 ewes to Grootfontein College of Agriculture at
Middelburg, Cape.
The Karakul is a fat-tailed sheep that produce lambs with beautiful fur pelts that are long lasting, non-perishable, and constitute an excellent economic proposition for farmers in dry areas with inadequate transport services. In general, the Karakul is a large, sturdy, prolific and mobile animal, with a long, slender head and an arched nose. Their fleece is dominantly black, but gradually turn gray with maturation; shadings of brown and white can also occur. Mature rams, weighing from 80 to 100 kg, usually have well formed horns, although some are polled. Ewes, weighing 45 to' 79 kg, are generally polled. The Karakul is hardy, longevity and adapts well to the arid regions of South
Africa and Namibia. Ewes breed out of season, generally drop three lamb crops in two years; a lamb percentage of 120 and more on well-managed farms can be expected.
Karakul ewes are very protective and attentive mothers. Their milk production is plentiful
and lambs can reach a mass of approximately 21 kg after 100 days. The Karakul tends to be flock bonded and non-selective feeders.
1-18 They are mainly bred for fur pelt production, with exceptional care to the pelt
quality of the lambs that reaches a phenomenal price at auctions. The breed also produces
a light fleece with strong fibers. Adult sheep are usually shorn twice a year, and their
fleece is very popular in the cottage industry. Namibian carpet weavers use Karakul wool
for their products. This breed is also a source of by-products, including milk and tallow.
They also play a vital role in maintaining the ecosystem in the more arid regions of South
Africa and Namibia, where the annual rainfall seldom exceeds 150 mm. Their broad tails
store fat, which becomes a source of nourishment when grazing is scarce.
Karakul is mostly found in the North-Western Cape and southern parts of Namibia.
South Africa markets almost half of all the Karakul pelts in the world (Terblanche, 1978;
Farmers Weekly, April 3, 1998).
Above: A Karakul ram showing of his perfect pelt (Farmers Weekly, April 3, 1998)
1-19 Above: Karakul ewe and her lamb (before it was shaved; Terblanche, 1978)
Below: A Karakul lamb after it was shaven (Terblanche, 1978)
• . r '
1 - 20 2.9 MERINO
The Merino is one of the most important and numerous breeds of sheep in South Africa.
This breed originated in Spain as a fine-woolled sheep. The King of Spain presented two
Merino rams and four ewes to the government of Holland. In due course they sent a small flock to the military commander of the Cape, Colonel Gorden, who was keen to determine how the woolled breed would adapt to local conditions. Unfortunately he died six years later and the flock was offered for sale. Some of the sheep were purchased by passengers on two ships at anchor in Table Bay, bound for Australia, and these in due course became the foundation stock of the local Merino industry. South Africa later began to import Merinos from Australia for the improvement of local flocks. These imports have increased by leaps and bounds since about 1910, which worried the
Australians to such an extent that they placed an embargo on further exports from their country.
The Merino is a white-woolled sheep breed that holds fine fleece and consists of a reasonable mutton conformation. The horns of Merino rams are traditionally massive and impressive, and generally stand well away from the head, in contrast with the horns of the ewes, which are not regarded as very important, and it is not uncommon to find both horned and polled ewes in the same flock. Adult rams and ewes can reach a mass of
85 and 50 kg respectively. A lamb percentage of 80 can easily be attained, although
extreme management could improve this statistic. The wool is of a high quality and
spinning count varies from very strong to ultra-fine. Adult ewes produce 5 kg of wool in a
good growth year, while adult rams could produce up to 10 - 15 kg or more.
1-21 The Merino is a double purpose sheep breed, mainly being selected for its wool, but also for the production of red meat. The carcasses is mainly being marketed as adult wethers (rams without testicles) and old ewes, although cross lambing can also serve as contribution. Old, adult ewes and wethers reach a live mass of 45 and 50 kg respectively in good conditions. A lamb carcass can reach 15 kg at an early age of five months.
The Cape is the most important wool producing area in South Africa. The Merino is the most common sheep breed in the world, with large numbers in Australia, Russia,
Nieu-Zealand, Argentina and South Africa (Terblanche, 1979 and Farmers Weekly,
March 1998).
Below: A flock of shaven Merinos (Farmers Weekly, March 1998)
1 - 22 Above: A flock of unshaven Merinos (Terblanche, 1978)
1 - 23 2.10. NAMAQUA AFRIKANER
Although the Namaqua Afrikaner is a South African breed, believed to be descended from the fat-tailed sheep kept by the Hottentots and Koranas who lived an iolated life in the semi-arid north-western Orange River region of the former Cape Colony, only an estimated 2 000 head are left today. This breed was near to extinction in 1950, a conservation programme saved it. The remaining 'flocks were kept by the Department of
Agriculture for the specific purpose of preserving the genetic pool and recording production and reproduction data. More interest was shown by farmers to develop this breed and a surplus amount were made available to few, farmers.
The Namaqua is a long-legged sheep that can walk long distances in search of grazing and water. They can also tolerate intense heat as well as low night temperatures.
They are-covered-with-smooth shiny hair, and the head color can- range-from-black to brown to red. Most Namaqua sheep have corkscrew tails that either turn right or left at the hocks. The Namaqua is a virile sheep breed that has a long life span and their production and reproduction performance recorded under extensive conditions compares favorably with other South African breeds. By mobilizing their fat reserves, the ewes are able to wean heavy lambs even under severe drought conditions. Their mothering ability are excellent, and they will fiercely protect their offspring against all intruders, whether human or predator. Ewes are prolific, mature early and have an average lambing cycle of nine months. The ewes can lamb throughout the year, at an early age. Lambs weigh approximately 4 kg at birth, and wean at 25 kg.
1 - 24 The Namaqua Afrikaner carcass is narrow, with more localised fat in the tail and rump area. The Namaqua sheep skin is suited for light mass leather goods such as gloves and jackets (Farmers weekly, March 1998). The perception of small flock research scientists is the this hardy indigenous breed, only found in South Africa, are of little actual value. This, however, is an extreme shortsighted view (Campbell, 1995).
Above: Namaqua Afrikaner ram (Farmers Weekly, March 1998)
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Above: Namaqua Afrikaner ewes and lambs (Farmers Weekly, March 1998)
1 - 25 2.11. PEDI
The Pedi can be classified as an indigenous fat-tailed breed from South Africa. They originated in the old Far North-, Northwest- and Northeastern Transvaal. Today their distribution is much broader. Breeders are found in the Northern Province, Northwest,
KwaZulu-Natal and Swaziland. As far as we know, the earliest African people farmed with this breed. Their sheep had to survive without any veterinarian aids, and nature was the selector, inspector and vet. Animals that could not resist ticks, heart water and parasites died. In this manner nature, with its extreme selection, developed a sheep breed that is extremely tolerant to diseases.
Sheep are mostly white with brown heads. Although, the Pedi could be a hodge. podge of colour, entire black sheep are also found. Their long, strong legs allow them to walk long distances in search of food. They have a long tail that sometimes reaches the sheep's heal; some tails turn back while others turn forward. This breed has no horns and their noses are uncinated with a well-developed jowl. Lambs are born with smooth hair, but after three months it becomes a wool-like coat, that shed after a few years. The adult sheep has a smooth coat that becomes woolly after the winter to protect the sheep against the cold weather. The Pedi has excellent mothering ability and produces twins without difficulty.
Most occur in the Soutpans Mountain in the Northwest Province Terblanche,
1978).
1 - 26 Below: Pedi ewes with their lambs grazing on a thorn bush. Although they do eat grass, they prefer bushes and leaves (Farmers Weekly, August 1998)
Below: A very good example of a-Pedi-ram-and ewe with broad backs (Farmers Weekly, August 1998)
1 - 27 2.13. ROMANOV
"Romanov Sheep - Lambs by the litter" is how this breed is known across the world.
Romanov sheep are from the Volga Valley, northwest of Moscow. Genetically unique to
North American and British breeds of sheep, the Romanov (a "pure gene" - not a cross of anything) used on traditional ewes, will produce a hybrid of high performance, for the flock of the future and a top gaining market lamb." Microsoft Internet Explorer, 1996).
This is an exotic sheep breed, with some purebred Romanov sheep flocks in South
Africa. A control group is kept at the Irene, Animal Improvement Institute near Pretoria, managed by mr. Schalk Nieuwoudt, head of the sheep section. It is managed and _ maintained with the primary objective of providity of limited genetic material. A second objective is to make all surplus animal's available to potential local breeders.
Internationally, the Romanov is regarded as one of the most prolific mutton breeds.
Romanov sheep have a phenomenal early sexual maturity. Rams and ewes are fertile by the time they are three months old. Romanov ewes will breed any month of the year. This out-of-season breeding ability, combined with the multiple birth ability allows the high production of a Romanov ewe. It is common for the Romanov ewe to lamb three times in two years, with quadruplets, quintuplets and even sextuplets being a normal occurrence. The ewes are known for their trouble-free lambing, even with multiple births.
The newborns are aggressive and this ensures their survival and entails an impressive lamb crop.
Purebred Romanovs are born black and lighten to a soft silver grey. Most
Romanov lambs are born white, although a few are born tan, grey or speckled. Ewes can
1-28 reproduce successfully up to the age of ten years, although the peak reproductive period is three to four years old.
The Romanov is essentially a mutton breed, but it also produces a fleece consisting of fine, white wool interspersed with coarse black hair, the quality of the wool does not compare well with that of other woolled breeds, but there is a market for it, for example in the manufacture of carpets. Previous cross breeding with other mutton breeds like the
Dorper and Blackhead Persian were extremely successful. Thus developing a well- conformed mutton sheep for small-scale farmers (Farmers Weekly, March 1998; Microsoft
Internet Explorer, 1996).
Below: A group of maturing Romanov rams about to be sheared (All figures shown were taken from the March 1998, Farmers Weekly)
1 - 29 Left: Schalk Nieuwoudt, head of the Irene Animal Improvement Institute's sheep section, with a five week old Romanov ram lamb.
Below: Part of the Romanov flock being herded into the central handling yard for visual appraisal
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1 - 30
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Above: A two-year-old Romanov ram
1 -31 2.14. RONDERIB AFRIKANER
The Ronderib Afrikaner is a variety of the Blinkhaar Afrikaner. This is an indigenous long- legged sheep breed. The name Ronderib is derived from the fact that the rib itself is oval in shape. This breed is very well adapted to arid conditions. Water intake is reduced by grazing on small annual succulent plants available in the Kalahari.
This is a fat-tailed mutton breed with a white creamy covering, consisting of hair and wool. A soft silky white downy wool is found on the undercovering of the skin, while the fleece contains soft, long hair. Adult rams and ewes reach a life mass of 80 and 60 kg respectively in extensive field conditions. Amber colored horns, with one and a half turns, are found on the rams, while the ewes can also have small horns. Its body shows a square form; this is because of the prominent chest. The rams has visible mane hair underneath the neck and the lower end of the chest. The head is long and narrow and presents a concave profile. The tail consists of three parts, is very distinctive, could reach a mass of '8 - 12 kg and hangs just above the heel.
The fat_tails of the Ronderib Afrikaner are used during the_winter_months, when it is time for game to be slaughtered, as processed foods. The Ronderib Afrikaner contributed to the development of the Vandor and Van Rooy breeds. The skin is sought after for the make of skin (karos) blankets. The silky soft mixture of hair and wool makes it very suitable for this purpose, furthermore it qualifies as "Cape Glover" which fetch high prizes, and it is used in the making of thin leather gloves. Ronderib: Afrikaners are mostly found in Richmond, Philipstown, De Aar, Laxton and Graaff-Reinet.
1 - 32 Below left: Ronderib Afrikaner ram Below right: Ronderib Afrikaner ewes and lambs (Landbouweekblad, March 1998) Far below: The fat tail and shiny fleece can clearly be seen. (Campbell, 1995)
I. - 33 2.15. RUSSIAN RED WOOLLED PERSIAN
The Russian Red Woolled Persian were imported from Romini. came to South Africa. It has no relation to the Blackhead Persian or Redhead Persian what so ever. It could be classified as an exotic fat-tailed sheep breed.
The Russian Red Woolled Persian is covered with a combination of wool and hair.
It has a prominent fat tail with a similar conformation to the Ronderib Afrikaner sheep breed. Big forward turned horns can be found on the ram; this breed displays a brown color with black legs. The ewes are polled and have a slightly darker head.
The distribution of this breed is limited. It is however well adapted to Fankfort in the Free State.
Below: Russian Red Woolled Persian rams
1-34 2.16. SKILDER OR SPECKLED PERSIAN
The color pattern of the Skilder Persian can be black or brown with white spots covering the body. The speckled color of the Persian appears to be a recessive color of fat-rumped sheep. Epstein (1971) stated: "The coat color is basically white with a second color, usually light to dark brown superimposed." Some show an admixture of bright brown hair.
In East Africa the fat-rumped sheep is essentially an inhabitant of dry areas. Red speckled lambs can be obtained by mating a Blackhead Persian or a Redhead Persian ram to white
Van Rooy or Ronderib ewes (Campbell, 1995).
Below: Two Skilder Persian ewes
1-35 2.17. SOUTH AFRICAN MUTTON MERINO
The South African Mutton Merino is one of the most important sheep breeds in South
Africa. They originated due to the cross breeding between the Dutch Merino and the Mele sheep breed in 1932. After 1946 the name of the breed was changed to South African
Mutton Merino, because the Dutch Merino could no longer be imported for breeding purposes, thus the breeders used indigenous material resulting • in an adapted composite breed.
The. South African Mutton Merino is a big dual purpose sheep with good conformation, mutton characteristics and alot of white wool of high quality. The Merino wool is an attractive weight wool with excellent standing power and without any kemp. In the modern classification of sheep breeds, the South African Mutton Merino will be considered a dual-purpose sheep. This breed has as exceptional mutton conformation and could reach a mass of 110 kg. It is very versatile and it is able to adapt to all types of grazing and climate conditions. A strong jaw and broad teeth, places this breed in a class of less fastidious grazing habitude. Breeding capability_is one of the breeds best . characteristics, with a lamb percentage of 150 or more and the capability to produce more than one lamb is a common occurrence. The immanent growth potency together with the high milk production of the ewes ensure a rapid growth rate of lambs and a mass increase of 0.4 kg per day in good feeding conditions. Lambs can reach a mass of 34 kg after 3 months in good field conditions. The lambs are vigorous and strong straight after birth.
The South African Mutton Merino could mate any time of the year.
1-36 The South African Mutton Merino is bred for its wool and meat. Lambs can be marketed at three and a half to five months of age under intensive conditions with a carcass mass of 16 to 25 kg. This breed contributed to the breeding programs of the
Dormer, Vandor and Dohne-Merino. It is commonly used in crossbreeding and development programs. The adaptability of this breed has shown that it can tolerate a broad spectrum of field and climate conditions and its immaculate characteristics which are highly heritable with crossbreeding. They are found throughout southern Africa.
(Terblanch, 1979).
Below: Champion Mutton Merino ram
1 - 37 Below: South African Mutton Merinos (Farmers Weekly, August 1996)
I. - 38 2.18. VANDOR
In the early 1940s, mr. S. van Vuuren, a farmer of Philipstown identified a need for a white-woolled mutton sheep and began an intensive breeding program that eventually culminated in the evolution of the Vandor. Mr. Van Vuuren used the Van Rooy ewe as the maternal basis of his breeding program, primarily because of hardiness and excellent mothering ability. The Dorset Horn ram was used because of its adaptability, great conformation and mass, good milk production of its female progeny, and its white-woolled fleece. Then, for two seasons, he introduced the German Merino to remove the last traces of horns, localisation of fat and kemp in the wool, and to improve the mutton quality. This stringent seleation lasted nearly 30 Years. During the drought years of the 1950s, 1960s and 1980s, the Vandor survived successfully. Vandor rams are under strict selection and these are offered for stud breeding or for crossing with any breed, ,especially with. Merino,
Dorper, Blackhead Persian and Van Rooy ewes.
The breed thrives in all areas, from the high rainfall parts of the eastern Free State and Mpumalangato_the extensive southern parts of Namibia and the _Northern_Cape. The
Vandor is an exceptionally fertile nonseasonal breed, requiring only one or two rams per
100 ewes to produce 150% lambing in a normal season. The rams remain active throughout the year. Rams generally gain a weight of 40 to 45 kg in 100 to 120 days and ewes gain 35 to 45 kg during the same time. The lambs grow rapidly, gaining between 300 and 400g a day to produce an exceptional carcass. The breed has a distinct white face, large ears, good eyelid pigmentation, strong mouth and white legs. It prbduces a light
1 - 39 wool fleece averaging about 2 to 3 kg at 12 months. Although slaughter lamb production
is its main economic function and its fleece is of secondary importance, the wool invariably realizes prices in the upper bracket (Farmers Weekly, May 1, 1998).
Below: Champion Vandor ewe (Farmers Weekly, May 1, 1998)
I.- 40 2.19. VAN ROOY
Senator J.C. van Rooy developed the Van Rooy breed during 1906 near Bethulie. The
Van Rooy is an adapted synthetic sheep breed and is the result of the crossbreeding between a Blinkhaar Ronderib Afrikaner ram and a Rambouillet ewe to ensure a hardy fat tailed sheep breed. The Van Rooy is a big white fat-tailed mutton breed. It is covered with wool and bristle. Both the rams and ewes have dewlaps with a prominent chest. The tail is broad and attached high up on the rump, which also classifies this breed as a fat-rumped breed. The tail is used in processed foods like sausages. The breed is mainly found in the
Cape Province (Campbell, 1995).
Below: A flock of Van Rooy sheep
1-41 3. THE OBJECTIVES OF THE STUDY
South Africa is rich in many indigenous and well adapted sheep breeds. It is therefore necessary to document the genetic variation and relationships of the different breeds. The following aims were identified:
1. The primary aim of the present study is to genetically characterise 19 sheep breeds from southern Africa; by comparing genotype and phenotype frequencies. The following polymorphic serum markers were used: albumin (ALB), carbonic anhydrase
(CA), diaphorase (Dia), haemoglobin (HB), transfefrin (TF) and X-protein (X). Another eight enzyme coding loci were analysed for the Dorper, Van Rooy, Romanov, Dormer. and South African Mutton Merino breeds: esterase (EST-1, -2; E.C. 3.1.1.1), glucose dehydrogenase (GDH; E.C. 1.1.1.47), glycerol-3-phosphate dehydrogenase (GPD; E.C.
1.1.1.8), glucose-6-phosphate isomerase (GPI; E.C. 1.1.1.27), lactate dehydrogenase
(LDH; E.C. 1.1.1.27), peroxidase (PER; E.C. 1.11.1.7) and 6-phosphogluconate dismutase (PGD; 1.1.1744). The additional loci were analysed to evaluate the accuracy of the initial phenetic relationships obtained from only the six polymorphic loci used in routine analysis.
: 2. Random Amplified Polymorphic DNA analyses were also attempted to compare the sheep breeds.
Genetic and morphological information were utilised to strengthen inferences about specific aspects of population structure, and effective gene flow.
Information for future monitoring of gene flow in populations are provided to
enhance the global information on domestic animal diversity.
1 - 42
CHAPTER 2 MATERIALS AND METHODS 2-2 Introduction 2-2 Sampling 2-3 Biochemical techniques 2-4 Histological techniques 2-5 Nomenclature 2-6 Statistical analysis 2-7 Random amplified polymorphic DNA 2-8 DNA extraction 2-8 PCR 2-9 Agarose gel 2-10 RESULTS 2-12 Analysis of six polymorphic loci 2-12 Additional loci 2-14 Random amplified polimorphic DNA 2-16 DISCUSSION 2-22 Genetic variation 2-22 Genetic differentiation 2-25 Random amplified polimorphic DNA 2-27 Conclusion 2-27
2 - 1 CHAPTER 2 MATERIALS AND METHODS
1. INTRODUCTION
Starch gel electrophoresis and Random Amplified Polimorphic DNA (RAPDs) were used
to determine the genetic variation between the sheep breeds. An average of 100 animals
were sampled from 19 sheep breeds. The breeds analysed, their status and sample sizes are
presented in Table 2.
Table 2. Breed number, sheep breed, sample size (N) and status of each breed studied.
NO BREED N STATUS 1 Dorper 151 Adapted synthetic breed 2 Pedi 100 Indigenous 3 South African Mutton Merino 97 Adapted synthetic breed 4 Landrace 99 Exotic 5 Merino 99 Adapted breed 6 Afrino 100 Adapted synthetic breed 7 Namaqua 169 Indigenous 8 Van Rooy 180 Adapted synthetic breed 9 Russian Red Woolled Persian- — 105 Exotic 10 Border Leicester 71 Exotic 11 Blackhead Persian 217 Indigenous 12 Ronderib Afrikaner 138 Indigenous 13 Damara 98 Indigenous 14 Skilder Persian 52 Indigenous 15 Dormer 286 Adapted synthetic breed 16 Vandor 31 Adapted synthetic breed 17 Karakul 25 Indigenous 18 Perinoff 12 Adapted ,synthetic breed 19 Romenov 28 Exotic
Samples used were obtained from three different breeders per breed (unrelated
animals). The breeders were mostly from different geographical locations across South
2 - 2 Africa where the breeds occur. Locations included Elsenburg test station, Bloemfontein agricultural farm, Mr. Read, a successful farmer at Settlers, Roodeplaat Dam test farm and a few small-scale farmers in the Eastern, Northern and Western Cape.
2. SAMPLING
Due to economic implications, it was not feasible to sacrifice any of the sheep that were sampled for research purposes (because most breeders • were stud breeders). It was therefore imperative that the sampling process involved a non-critical tissue type and that the extraction thereof caused minimal stress to the sheep (farmers are very sensitive and protective of their animals). Blood has also proved to be a favourable tissue source for electrophoresis by many researchers in the past (e.g. Matson, -1984).
Blood samples were obtained by extracting blood from the femoral vein of the hind leg after disinfection of the area with 70% ethanol, as recommended by Evans (1987). The reason is because the wool in the neck area of the sheep is too thick to successfully reach the aorta. A 4 ml sample was taken from each individual with a 10m1 Promec syringe and a 18 g hypodermic needle, where after it was directly placed into a 4.5m1 glass vacutainer (test-tube). The glass test tubes respectively contained 0.05m1 heparin (Barrowclaugh and
Corbin, 1978), 0.20m1 saline (0.9% NaCl - Manwell et al, 1963) and ethylenediamine- tetracetate (EDTA 10% - Corbin et al., 1974) to which 4 ml of blood was added. The glass test-tubes were immediately placed in crushed ice for transportation to the laboratory. The samples were centrifuged for 10 minutes at 2 000 rpm (Evans, 1987) to separate plasma and red cells. The plasma was drawn from the red cells and transferred to
2 - 3 alternative test tubes. The plasma, red cells and whole blood samples were frozen and stored at -20°C to await electrophoresis.
3. BIOCHEMICAL TECHNIQUES
A powerful biochemical method, electrophoresis, was used to enable the detection of mobility of proteins. The term "electrophoresis" is generally defined as the migration of particles in a medium with an electrical charge. In the case of proteins, the overall charge
originated form the carboxyl ( - COOH) and amino (-NH2) groups, which can assume an electric charge according to the pH of the medium.
Hydrolysed horisontal starch gel was used and the standard protocol of flat bed starch gel-electrophoresis was utilised to separate allele products (Baker et. al., 1966).
Buffer systems and gel concentrations described by Shaw and Prasad (1970) were used to analyse six enzymes (ALB, Dia, CA, HB, TF and X). For TF, tris-boric acid was used as buffer (pH 8.7); in the two-dimensional electrophoresis, tris-citric acid was used as buffer
(pH 6.3) for separating ALB; for CA and X, boric-NaOH was used as buffer (pH 8.7) and a tris-boric acid buffer was used for HB and Dia but with pH values of 8.9 and 8.6 respectively. The following buffer systems were used to analyse the additional eight
enzymes: a) MF - a continuous Tris, boric acid and EDTA • buffer system (pH 8.6 -
Markert and Faulhaber, 1965; for GLD, GPD and PGD) and b) RW - a discontinuous, citric acid (gel pH 8.7) lithium hydroxide and boric acid buffer system (electrode pH 8.0;.
Ridgeway et al., 1970; for EST - 1, 4, GPI, LDH and PER). Twelve percent starch gels
(42 g starch in 350 ml buffer) were used to resolve these eight enzymes.
2 - 4 The 12% starch (Sigma, S4501) gel were moulded and a piece of glad wrap was placed over it after the gel has cooled down and set to ensure that it does not dry out before it was used. A small amount of sample (blood) was extracted by means of a tiny piece of filter paper that was inserted into the support medium, the gel, with an appropriate pH depending on the protein stained for. The ends of the gel were connected to a suitable electrolyte solution and the electric field was applied for at least five hours of the time that it took to obtain the best resolution.
Electrophoretic migration also depended on a number of parameters other than the electric charge of the molecule: size, shape, concentration, degree of hydration and dissociation of the molecule; viscosity, pH, temperature and ionic strength of the medium, electric field strength and migration time.
4. HISTOCHEMICAL TECHNIQUES
Following electrophoresis, the starch gels were sliced into four layers and each layer was assayed for a different protein. The gels were stained with a general protein dye or with a specific enzymatic staining solution. Staining recipes adapted from Harris and Hopkinson
(1976) were used. The enzymes stained for were: glucose dehydrogenase (GLD; E.0
1.1.1.47), glycerol-3-phosphate dehydrogenase (GPD; E.0 1.1.1.8), 6-phosphogluconate
(PGD; E.0 1.1.1.44), esterase (EST- 1, - 2; E.C3.1.1.-), glucose-6-phosphate isomerase
(GPI; E.0 3.5.1.9), lactose dehydrogenase (LDH; E.0 11.1.27) and peroxidase (PER;
E.0 1.11.1.7).
Two methods were used to apply the stain to the gels. The first entailed soaking the gel in a solution containing the staining components, followed by destaining the gel in
2 - 5 a second solution that was devoid of the staining agent (Grant, 1989). The second method was performed by using a two percent agar overlay in which the staining reagents were mixed. The latter method is used for detection of the majority of enzyme systems in starch gels (Evans, 1987). With these procedures, the different variants of a protein, if characterised by a different overall electric charge, could be identified on the basis of their different electrophoretic mobility. Enzymes differing in electrophoretic mobility as a result of allelic differences in a single gene are called allozymes. Thus, allozyme variation in a population is an indication of genetic variation. Extensive allozyme variation has been found in virtually all natural populations studied by electrophoresis (Selander et al., 1973).
After staining, the gels, electropherogrames were drawn and the stained gels were
photographed.
5. NOMENCLATURE
Enzyme or protein commission numbers, names and abbreviations were allocated
according to the recommendations of the International Union of Biochemistry and
Nomenclature committee. Six loci for each breed, and thereafter an additional eight loci
for five breeds were studied with a maximum of nine alleles per locus. Alleles are referred to as detectable electrophoretic variants that differ genetically from one another at a locus,
according to international comparison test ISAG nomenclature: Alleles at each locus were
alphabetically designated by the electropheretic mobilities of the gene products they
encode.
6. STATISTICAL ANALYSIS
2 - 6 The BIOSYS-1 computer program (Swofford and Selander, 1981) was used to calculate a number of statistical parameters. The allele frequencies at each locus were computed and the Chi-squared (x2) values for deviations of allele distribution from expected Hardy-
Weinberg equilibrium was tested at each locus. The critical values can be found in a x 2 table with the same degrees of freedom (Ferreira et al., 1984), where the rejection criterion for one degree of freedom has a value of 3.841 (Stoker, 1977).
The idea of allele frequency supports the notion of polymorphism. A polymorphic gene is a gene at which the most common allele has a frequency of less than 0.95. The cut off at 0.95 in the definition of polymorphism is arbitrdry.
The average heterozygosity (H) is estimated by totalling the number of heterozygous individuals for each gene, dividing this by the total number of individuals in the sample, and averaging over all genes. The observed heterozygosity of individuals (11) was calculated as the ratio of the number of heterozygous loci within the individual to the total number of loci assayed for that individual (Nei, 1978). The coefficients of heterozygosity deficiency or excess (d) for each polymorphic locus, mean number of
alleles per locus (A), and percentage of polymorphic loci (P) using the 0.95 criterion were
calculated. P was estimated as a ratio of the counted number of polymorphic loci to the total number of loci studied and expressed as a percentage (Corbin, 1983). Wright's
(1978) fixation index (F) values for polymorphic loci were calculated at each locus.
The genetic distances (D) between breeds were determined with Nei's (1972,
1978) formuli, and DISPAN (Ota, 1993) was used to construct a dendrogram from Nei's
(1978) genetic distance values using neighbour-joining and bootstrap tests (1 000
2 - 7 replications). GENEPOP (Version 3.1b, Raymond and Rousset, 1995) was used to
determine if significant (P<0.05) differences in allele distribution were present between
breed pairs.
7. RANDOM AMPLIFIED POLYMORPHIC DNA
a. DNA extraction
Initially, several methods were tested to extract enough DNA from the blood samples. The
high pure PCR template preparation kit (available from Boehringer Mannheim GmbH)
was the only method that gave purified nucleic acids from the whole blood.
A volume of 200 IA whole blood was pipette into a 1.5 ml reaction tube, 200 ill
PBS, 200 p.1 binding buffer (supplied with the kit) and subsequently 40 µl proteinase K
(supplied with the kit) were added to the sample. Different micropipettes were used to pipette the solutions, the PBS was added with a_ syringe_needle and kept below 20°C (all
the other solutions were kept at room temperature, but only lasts 12 months). This
solution was vortexed and incubated in a standard electric regulated warm bath at 72°C
for ten minutes. After incubation, 100 Al isopropanol were pipette into the sample and
vortexed for five seconds. The high pure filter tube and collection tube, supplied in the kit;
were combined and the sample was pipetted into the upper reservoir. This was centrifuged
in a standard tabletop centrifuge at 8 000 rpm for one minute, and the resulting flow
through was discarded into a septic dustbin. The filter tube and used collection tube were
combined again and 500 pl wash buffer (supplied in the kit) was added to the upper
2 - 8 reservoir. This was centrifuged at 8 000 rpm for one minute. This step purifies the DNA from salts, proteins and cellular impurities. The flow through was discarded, the filter and collection tube combined again, 500 pl wash buffer added and centrifuged at 8 000 rpm for one minute. To remove residual wash buffer, it was centrifuged a further ten seconds at maximum speed (13 000 rpm). The collection tube was then thrown away and the filter tube inserted into a clean 1.5 ml reaction tube. Finally, 200 p.1 preheated (72°C) elution buffer (supplied in the kit) was added to the filter tube and centrifuged for one minute at 8
000 rpm. This was done to ensure the elution of the nucleic acids. The extracted nucleic acids (DNA) were then ready for the polymerase Chain reaction (PCR), however it is
stable and can be stored at 4°C for future analysis.
b. PCR
The PCR is a process for the artificial synthesis of nucleic acids. It involves the in vitro
amplification of particular DNA sequences with the help of specifically or arbitrarily
chosen oligonucleotides ("primers") and a thermostable DNA polymerase, the
electrophoretic separation of amplified fragments, and the detection of polymorphic banding patterns by such methods as staining. Amplification reactions were performed as
described by Williams et al. (1990).
A DNA sample of 2.5 pl were pipetted into a 0.5 ml reaction tube. The following
PCR reaction solutions were added to the DNA: 14.7 IA double distilled water, 1.6 p.1
dNTP, 2.5 pi magnesiumchloride, 2.5 pl 10X buffer, 0.2 IA Takara Taq (coenzyme
initiating amplification of the DNA) and 1.0 µl primer. Operon OPA-1 to OPA-10 primers was used for each sample after all OPA primers were initially analysed. These solutions
2 - 9 were pipetted by using a very accurate micropipette. Caution and sterility was extremely
important because any form of contamination will alter results. Surgical, sterilised gloves were worn throughout the duration of the practical experiment to minimise contamination.
The solutions were carefully vortexed, and extreme caution was taken to ensure all the
drops adhering to the side of the reaction tube has run down to the bottom of the tube.
The reaction tubes containing the DNA and PCR solutions were then placed in the
Omn- E thermal cycler. The thermal cycler was programmed to convert to specific temperatures. During the first minute, the tubes were subjected to a temperature of 94°C, this caused the double DNA helix to break or split ih two (denaturing of the DNA). The temperature was then lowered to 36°C for a minute, this enabled the shot primers (ten base pairs) to anneal to the single stranded DNA. Finally, the temperature was raised to
72°C. It was now possible for the DNA to amplify according to the sequence of the added
primer. The cycle was programmed to repeat 45 times.
c. Agarose gel
A 2 % agarose gel was prepared, by adding lg agarose to 50 ml TBE (2.75 g / 500 ml
Boric acid, 5.39 g / 500 ml Tris and 0.58 ml / 500 ml EDTA) in a 250 ml glass bottle. The
cap was loosely put on top of the bottle and placed into a microwave oven for a few
seconds until the agarose was dissolved. The cap was then tightened properly, and the
solution cooled with cold water from a tap. After degassing, the cap was taken off and 5
1.11 ethidiumbromide was added to the solution (this is the colourant used to visualise the
migrating DNA). It was shaken in order to mix the ethidiumbromide with the solution, and poured into a perspex mould. While the agarose gel was solidifying, the TBE running
2-10 buffer was poured into the gel electrode container. The moulded gel was then placed into the gel electrode container and filled up with running buffer (it is important for the whole gel to be covered with the buffer to ensure that the electric current runs through the entire gel).
Twenty micron amplified DNA were pipetted onto wax paper and mixed with a blue dye to produce a more visible substance. Each individual sample was pipetted into a different slot of the moulded agarose gel. After all the DNA samples were pipetted into the gel, the lid of the gel electrode container was closed, and an electric current of 80 V was connected to the electrodes. This process ensures the migration of the DNA, and was allowed to run for approximately four hours.
After four hours, the electric current was shut off, the gel taken out of the electrode container and placed on top of an electric neon light to view the migration of the
DNA. The results of the gel was then finally scanned into a computer and saved on a disk for future analysis.
2 - 1 1 RESULTS
1. ANALYSIS OF SIX POLYMORPHIC LOCI
All six loci were polymorphic in this study; the relative allele frequencies for 19 sheep breeds
from southern Africa for each polymorphic locus are given in Table 3. Loci where significant
(P<0.05) deviations of allele frequency from expected Hardy-Weinberg proportions occurred
were estimated (Table 3). The D-allele is the most common allele at the TF system for most of
the breeds, except for the South African Mutton Merino and Perinoff (A-allele), Russian Red
Woolied Persian and Border Leicester (B-allele) and Karakul (C-allele) breeds; the H-allele
was exclusively found in the Dormer breed, although at a low frequency, and the G-allele was
found only in the Afrino, Van Rooy, Border Leicester, Blackhead Persian and Skilder Persian
breeds. Only seven of the 19 breeds' allele classes did not comply with Hardy-Weinberg
expectations at this locus (Table 3).
All of the breeds, except for the South African Mutton Merino and the Van Rooy
breeds, were monomorphic at the ALB system and only the South African Mutton Merino had
allele frequencies that complied with Hardy-Weinberg expectations at this locus. Thirteen of
the breeds were monomorphic for the B-allele at the CA system, C-alleles were observed for
the Russian Red Woolled Persian and Border Leicester breeds, whereas A-alleles were present
in the remaining four populations (Landrace, Afrino, Namaqua and Vandor). All of the
polymorphic breeds at the CA system had allele distributions that approximated Hardy-
Weinberg proportions. All of the breeds studied had the B-allele as the most common allele at these three loci (ALB, CA and X). All of the breeds where polymorphism occurred
2-12 have allele frequencies that did not comply with Hardy-Weinberg expectations at the X system
(Table 3).
The FIB system was polymorphic in all but the Pedi and Skilder Persian breeds, and the
Ronderib Afrikaner was the only breed with allele distribution that did not approximate Hardy-
Weinberg expectations at this locus. The Dia system was polymorphic, with most breeds showing the A-allele at the largest frequency, except for the South African Mutton Merino,
Perinoff and Romanov breeds (B-allele). Eight breeds did not have allele distribution that approximated Hardy-Weinberg expectations at this locus (Table 3).
The A, P, H and D values are given in Table 4. Values of 1.67 (Pedi and Romanov) to
2.5 (Afrino, Van Rooy and Border Leicester) were obtained for A, and H values ranged from.
16.6 (Namaqua) to 35.9% (Russian Red Woolled Persian) with an average of 27% for all the breeds. H represents an indirect estimate of their overall genetic variability. Between 50.00 to
66.67% of the protein coding loci were polymorphic in all the breeds, except for the Namaqua sheep (33.33%). The unbiased D (Nei, 1978) values were the smallest between the Dormer and
Dorper breeds (0.004) and also between the Perinoff and Romanov breeds (0.004), and the largest between the Romanov and Blackhead Persian breeds (0.140). The mean D value between the breeds was 0.067. These applications are especially valuable where rare breeds are concerned (Clarke et al, 1989).
2. ADDITIONAL LOCI
Seven of the eight additional loci were rnonomorphic for the five breeds studied. Only the PER locus was_polymorphic in all the sheep breeds, with A being the most common allele. None of the allele distributions approximated Hardy-Weinberg expectations at the PER locus.
2-14 Deficiencies of heterozygotes occurred in all of the breeds that did not have allele distributions that approximated Hardy-Weinberg expectations at the ALB, CA, X, HB, Dia and PER loci; with no heterozygotes observed at the ALB locus in the Van Rooy breed. Four of the breeds (Dorper, South African Mutton Merino, Blackhead Persian and Skilder Persian) had allele frequencies that did not comply to Hardy-Weinberg expectations at the TF locus due to an excess of heterozygotes. However, a deficiency of heterozygotes occurred in the other breeds at this locus. Individual heterozygosity values for polymorphic loci.ranged from 0.016 -
0.774 at TF,- 0.012 - 0.036 at ALB, 0.007 - 0.081 at. CA, 0.095-- 0.497 at X-protein ; 0.030 -
0.499 at HB and 0.168 - 0.499 at the Dia locus. Allele frequencies of South African Merino breed at the TF-locus were also compared with allele frequencies in Merino breeds in other countries (Table 5). The South African Merinos differed from Merino breeds in other countries at this locus.
The mean F value of individuals relative to the subpopulation (F is) is 0.260, 0.351 for the total population (Frr) and Fsr = 0.123 for the amount of differentiation among populations relative to the limiting amount under complete fixation (Table 6). The loci that contributed least to the inter- and intraspecific differences are ALB (Fsr=0.014) and CA (Fm=0.023) because all of the breeds studied shared the most common allele at these loci (Table 6).
Figure 1 presents a dendrogram that reflects the unbiased genetic distances between the breeds and their phylogenetic relationships. The closest genetically related breeds is the Dorper and Dormer breeds, Perinoff and Romanov breeds, Van Rooy and Skilder Persian breeds,
Blackhead Persian and Vandor breeds, Pedi and Damara breeds and Landrace and Afrino breeds, respectively (Table 4, Fig. 1). The results compared well with results obtained after the
2-15 additional enzymes were analysed (Fig. 2). The bootstrap values at the nodes increased with an average of 35%. No distinct genetic differentiation (Fig. 1) was obtained between the indigenous and exotic sheep breeds (e.g. the Landrace and Afrino breeds which are adaptive synthetic indigenous breeds).
Allele distributions were not identical between the following 25 breed pairs at all the loci studied: Dorper and Russian Red Woolled Persian, Ronderib Afrikaner and Romanov;
Pedi and Afrino, Blackhead Persian, Ronderib Afrikaner and Dormer; South African Mutton
Merino and Ronderib. Afrikaner; Landrace and Blackhead Persian and Dormer; Merino and
Afrino, Russian Red Woolled Persian, Blackhead Persian and Ronderib Afrikaner; Afrino and
Blackhead Persian; Namaqua and Russian Red Woolled Persian; Blackhead Persian and
Russian Red Woolled Persian, Karakul, Perinoff and Romanov; Ronderib Afrikaner and
Dormer and Skilder Persian; Dormer and Perinoff, Damara and Romanov; and Skilder Persian and Romanov and Perinoff.
3. RANDOM AMPLIFIED POLYMORPHIC DNA
Purified nucleic acids were obtained, which were clearly visible on the 2 % agarose gel. At first the OPA — 2 and OPA — 8 primers showed visible migrating nucleic acid bands, however after repeating the same procedure several times no corresponding successive results were found.
2-16 Table 5. Comparisoii of the frequency of transferrin alleles in seven populations of Merino sheep
Population No of Alleles Samples TF-A TF-B TF-C TF-D TF-E TF-G South African 81 0.469 0.012 0.173 0.148 0.198 Mutton Merino 4 South African 99 0.025 0.475 0.500 Merino 4 Mailliard Merino • 39 0.080 0:240 0.350 0.330 Peppin Merino 248 0.180 0.150 0.200 0.390 0.020 0.060 from Queensland A Peppin Merino 71 .0.110 0.080 0.190 0.460 0.080 0.060 from Badger), Creek * Tasmanian Fine 210 0.010 0.310 0.280 0.370 0.020 Woolled Merino A Spanish Merino v 200 0.190 0.150 0.120 0.437 0.102 4 Current study Stormont et al. (1968) Ashton and Ferguson (1962) Nguyen et al. (1992)
2-17
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2- 18 Table 6. Summary of F-statistics (Wright, 1978) at all loci for 19 sheep breeds from South Africa.
Locus Fls Frr Fgr TF 0.019 0.165 0.146 ALB 0.241 0.251 0.014 CA -0.029 -0.005 0.023 X 1.000 1.000 0.112 118 0.014 0.157 0.146 DIA 0.217 0.332 0.084 PER -0.066 0.047 0.113 Mean 0.260 0.351 .0.123
2 - 19 Figure 1. Dendrogram showing relationships between 19 sheep breeds using the unbiased genetic distance coefficient of Nei (1978). Numbers at nodes are boout ap values.
Karakul I Ronderib Afrilltaner
Thunarli 11
41 Blackhead Persian Vandor DorPer Dormer Van Rooy Skilder Persian Border Leicister Merino Russian Red Wooled Persian South African Meat Merino Perinoff
ROmena Figure 2. Dendrograin showing the relationships between sheep breeds using the unbiased • genetic distance coefficient of Nei (1978). DISCUSSION
1. GENETIC VARIATION
Cryptic polymorphism of blood groups or proteins, that have not deliberately been selected by man, show simple patterns of evolutionary change. For this reason the use of allozyme polymorphism can give accurate estimates of relationships between populations (Ordas and
San Primitivo 1986). Maintaining genetic variance within a pure breeding population is a complex problem. A possible solution would be to determine the degree of distribution of the current genetic diversity and can be used as a tool for conservation actions. This is an important consideration since the survival of some of the indigenous sheep gene pools are threatened by crossbreeding. The theoretical basis of the study of polymorphic proteins is that breeds can be defined as populations that differ from each other in the relative distribution and frequencies of genes (Hasselholt 1969).
This study genetically characterizes and describes 19 sheep breeds from South
Africa. Some differences in allelic constitution occurred between the sheep breeds studied, especially at the TF locus (Table 3); this indicates clear genetic differentiation between the breeds studied. For example, the TF*H allele is a rare allele exclusively found in the
Dormer breed (at a very low frequency) and the G-allele was only found in five of the 19 sheep breeds studied (Afrino, Van Rooy, Border Leicester, Blackhead Persian and Skilder
Persian breeds). Manwell and Baker (1977) suggested that electrophoretic variants with low frequencies may represent, in many cases, relatively recent mutations occurring after divergence of the breeds; this could be the case with the Dormer breed. The D-allele is the most common allele at the TF-locus for most of the breeds with a few exceptions already
2-22 - mentioned in the Results section (Table 3). The genetic differences between the breeds is to be expected for breeds found in separate locations throughout the Cape Province, where little or no gene flow occurs. The gene frequencies at the TF-locus were compared with those reported by other researchers to obtain information on the degree of divergence between Merino breeds (Table 5). Ashton and Ferguson (1962) reported the frequencies of alleles TF *A, -B*, -C*, -D*, -E* and -G* in three different populations of Australian
Merino; Stormont et al. (1968) reported the frequencies of these alleles in Mailliard Merino and Nguyen et al. (1992) published results on these alleles in Spanish Merino. Expressive differences can be noted between the Australian, Spanish, Mailliard and South African
Merino's. For example the TF*D allele appears to be the most common allele in all of the
Merino breeds, except for the South African Mutton Merino (showing a low frequency of
0.148 at this allele). The other Merino breeds had TF*B and TF*E alleles, but not the
South African Merino in the current study. Mailliard Merino also did not have TF*E and
TF*G alleles (Stormont et al., 1968). These differences could be due to small sample size
(39 Mailliard Merino), directed selection and different populations studied.
Six of the breeds were polymorphic at the CA-locus. The C-allele found in the
Russian Red Woolled Persian and Border Leicester breeds were at low frequencies; so the
A-allele found in the remaining four polymorphic breeds (Landrace, Afrino, Namaqua and
Vandor breeds) was also present in low frequencies at this locus. A study by Ordas and San
Primitivo in 1983 on Churra sheep breeds was compared with the current investigation.
They discovered a new allele (CA*M) at the red cell carbonic anhydrase locus in Churra sheep. They found the CA*S allele to be present in all of the breeds studied (Churra, Lacha and Manchega), and this allele was fixed in most of the breeds. In the current study, the
2-23 CA*B allele was also present in all of the breeds studied, it is therefore speculated that the latter allele is the same as the CA*S allele reported by Ordas and San Primitivo (1983).
The allelic constitution was similar at five (ALB, CA, X, Dia and PER) polymorphic loci, with three exceptions at the Dia locus. Three of the breeds. (South African
Mutton Merino, Perinoff and Romanov) had the B-allele at the largest frequenCy, whereas all the other breeds had the A-allele as the most common allele. This difference could be due to small sample size (especially for the Perinoff and Romanov breeds). Alternate alleles can be characteristic of breed.
Significant (P<0.05) . deviations of allele frequencies may occur due to crossing and linking, inbreeding, sample error, population bottlenecks and random genetic drift. Ideal
Hardy-Weinberg populations do not actually occur in nature due to various factors which can shift the equilibrium and disrupt the stability of a population, giving rise to change in the genetic structure. Since the sheep breeds studied do not represent natural populations, and because of directed selection of domesticated stock, it is not surprising that the allele frequencies deviate from Hardy-Weinberg proportions at most loci. Deficiencies of heterozygotes were found in all of the breeds that did not have allele distributions that approximated Hardy-Weinberg expectations (ALB, CA, X, HB, Dia and PER). This may be the result of strong (not deliberate) selection against heterozygous genotypes or the
Wuhland (1928) effect (the inclusion of two or more genetically distinct units into a single population sample). The directed selection criteria would be most appropriate in this case.
The values of P and H were the lowest in the Namaqua breed (P =33.33%, H. =
0.166; Table 4). Although A was calculated at 2.00 in this breed, it was not the lowest of the
19 breeds studied. This is due to four alleles being present at the TF locus in the Namaqua
2-24 breed, and only three TF-alleles in the Pedi, Merino and Romanov breeds, with lower A
values of 1.67, 1.83 and 1.67 respectively. Nevertheless, sufficient genetic variation exists
between breeds for selection purposes. The Russian Red Woolled Persian presented the
highest H value (0.359), and thus indicating a high genetic variation. The mean individual
heterozygosity values (0.774) were also the highest in the Russian Red Woolled Persian.
Selander and Kaufman (1973) and Clarke et al. (1989) reported P values of 10 to 20%. Our
. results of 50 to 66.67% is much higher due to only 14 loci being studied, whereas Selander
and Kaufman (1973) studied 32 loci and Clarke et al. (1989) studied 40' loci The latter
authors also reported an average H value between different sheep breeds of 19% (range: 13
to 53%); this compares well with the results obtained from the current study (16.6 to
38.9%). However, Selander and Kaufman (1973) reported H values of natural vertebrate
populations (rats, seals and humans) at approximately 6%.
2. GENETIC DIFFERENTIATION •
D values have been used to indicate genetic differentiation. The mean D value between the
breeds (0.067) indicates little genetic differentiation between the breeds. 'According to Nei
(1976) the D values for local breeds is between 0.000 and 0.058. Buis and Tucker (1983)
reported D values of 0.181 to 0.308 between different sheep breeds in England and an
average D value of 0.248. The topology of the dendrogram constructed from the matrix of
D values (Fig 1) allows us to differentiate two groups: 1) South African Mutton Merino,
Perinoff, Romanov and Russian Red Woolled Persian and 2) the remaining sheep breeds
studied. It can be inferred from the dendrogram that the flocks that are closest genetically
are the Dorper and Dormer, and Perinoff and Romanov breeds. This dendrogram was
identical to the one constructed after additional enzymes were analyzed (Fig. 2). It indicates
2-25 that the first six loci tested reflected true genetic diversity. The Dorper and Dormer breeds are commen ancestres (Terblance 1979). This association is also reflected in the dendrograms constructed from genetic distance values, and the high boOtstrap value (65) for the grouping of the Van Rooy and Skilder Persian breeds. This relationship can be explained because Skilder Persian rams are crossed with Van Rooy ewes to improve their gene pools (Campbell -1995). Similarly, the Blackhead Persian breed was introduced to upgrade the Vandor breed, which explains the small genetic distance value (0.007) between them (Farmers weekly, 1 May 1998). The basal position of the South African Mutton
Merino breed compared to that of the Dormer is' also supported by historical events. The crossbreeding of a Dorset Horn ram (with excellent mutton conformation and rapid growth ability) and a South African Mutton Merino ewe (with high fertility and the ability to produce more than one lamb) led to the origin of the Dormer.
Wright's (1978) fixation index is another measure to describe the level of differentiation between populations (i.e. a test whether or not they are from the same gene pool). The mean measure of the relatedness of individuals within populations (F sr) value of
0.123 for polymorphic loci (Table 6) is an indication of relatively little genetic differentiation between the breedt studied. The extent of allelic fixation of individuals relative to the subpopulations (F 1 = 0.260) also reflects the above phenomenon since values of F1 are less (close to zero) in most natural populations where random mating within subpopulations occurs (Nei 1986). In addition the FIT value of 0.351 (which quantifies inbreeding due to population subdivision) is indicative of relative little gene flow between populations. None of the breed pairs showed identical allele distribution at all the loci studied. Identical (P>0.05) allele distributions were obtained for 146 of the 171 breed pairs
2-26 and there is no obvious relationship between D values and allele distribution patterns. This is probably the influence of the small number of loci studied.
RANDOM AMPLIFIED POLYMORPHIC DNA
A single PCR primer designed at random will often by chance amplify several different regions of the genome. The single sequence "finds" DNA bracketed by two inverted copies of the primer sequence. The result is a set of different-sized amplified bands of DNA. In a cross, some of the amplified bands may be unique to one parent, in which case they can be treated as heterozygous loci and used as molecular markers in mapping analysis. The set of amplified DNA fragments (called a RAPD) is yet another type of DNA fingerprint that can be used to characterize individuals. Such identity tags can be very useful in routine genetic analysis or in population studies. At first the OPA— 2 and OPA — 8 primers showed visible migrating nucleic acid bands. However, RAPD results did not give consistent (repeatable) results. This is an expensive, time-consuming procedure with no real value in this study. It is also known that no consistent results can be produced between laboratories due to the varying conditions. The high risk of contamination is also a big problem, however every caution possible was taken to prevent contamination. It would be viable to use other methods (e.g., microsatellite, minisatellite, restriction fragment length polymorphisms —
FAO global programme, single-sequence length polymorphisms, etc.).
CONCLUSION
Allozyme studies can mesh genetic and ecological information to strengthen inferences about specific aspects of population structure, especially breeding structure and effective
2-27 gene flow. The results of this study presents A comprehensive study of the current genetic structure of the different sheep breeds found in southern Africa. Furthermore it is important for the future monitoring of gene flow in populations, to determine levels of hbreeding and crossbreeding in each breed, and to enhance the global information on domestic animal diversity.
2-28
SUMMARY
The domesticated sheep, Ovis aries, is classified as a member of the family Ovidae of the ord(
Artiodactyla. The earliest fossil records of sheep in Southern Africa dates back to about 2000 years ag( they appeared sporadically in the pleistocene fossil record and ice age. They migrated with earlic communities from the central lake of Africa. The sheep adapted very well to the environment and wei able to thrive on poor grazing. Sheep is therefore a hardy domesticated specie and can withstand extren- climatic conditions and are resistant to most diseases. There are approximately 1087 million shee worldwide.
The primary aim of this_study_was to genetically characterise 19 southern African_sheep breeds, b comparing them with respect to genotype and phenotype frequencies. Can aid in long-term managemer and meaningful conservation actions. Results indicated high genetic variation within and distin( differentiation between the breeds studied. For example, the Russian Red Woollecl Persian has the highe individual heterozygosity and genetic variation of all the breeds studied.
Genetic distances between the breeds indicated two groups (Figs:1 and 2; pp. 2-20 and 2-21) tin - correspond. Therefore, the additional eight loci analysed has proven the accuracy of the initial phenet relationships obtained from only six polymorphic loci used in routine analysis. The introduction of tr thesis presents some morphological and ecological information of the sheep breeds studied. The Karaku which is the oldest sheep breed in the world, unexpectedly did not resolve basally to the other she( breeds in Fig. 1. However, low bootstrap values (Fig. 1) indicate that the placement of the Karakul is, best, random. A Van Rooy ewe was used in the original breeding of the Vandor breed (see p. 1-39). Th close relationship is clearly shown, with a low bootstrap value (19%), in the dendrogram (Fig. 1), betwe( the two breeds. Both the Dorper and Dormer breeds were developed from a Dorset Horn ram. The highe
3 - 1 bootstrap values (67%) obtained between the Dorper and Dormer breed is, therefore, not unexpected. Th
Blackhead Persian played a crucial part in the development of the Dorper. Therefore, the basal clusterin of the Blackhead Persian, with the Dorper, Dormer, Skilder Persian, Van Rooy and Vandor is logical. TI
South African Mutton Merino was also used to develop the Dormer breed, which places the South Africa
Mutton Merino basally to the above-mentioned group. The Romanov and Perinoff breeds are exotic shee breeds, closely related and therefore clustered separately. Farmers crossbred the Damara with the Pec breed to improve their resistance against "hartwater", influence is also seen in Fig. 1. In summary, there congruency between the electophoretic results obtained (Figs. 1 and 2) and the breeding histories. Th above-mentioned information combined with the allozyme study, strengthens inferences about specifi aspects of population structure.
Unfortunately the RAPD analysis did not produce consistent (repeatable) results. Other method; for future genetic studies are recommended. However, the allozyme data aided in resolving some of th relationships of the breeds studied.
In conclusion, this study can aid in future monitoring of gene flow in populations, to determin levels of inbreeding and crossbreeding in each breed and to enhance the global information on domesti animal diversity for conservation. It is inevitable that the genetic characteristics of breeding stocks have t be enhanced, to identify selection criteria for economic growth and excellent mutton production. Mot efficient animals are needed with special adaptations to specific environments. After all, meat is food fc the nation and the ever-increasing human population size worldwide demands more research, effectiv and sustainable utilisation of all natural resources.
3 - 2
CHAPTER 4 REFERENCES
ASHTON, G.C. & FERGUSON, K.A., 1962. Serum transferrins in Merino sheep. Genetical Research, Cambridge 4, 240-247. BAKER, C.M.A., MANWELL, C., LABRISKY, R.F. and HARPER, J.A., 1966. Molecular genetics of avian proteins - V. Egg, blood and tissue proteins of the Ring- necked pheasant, Phasianus colchicus L. Comp. Biochem. Physiol.,17 , 467-499. BARROWCLOUGH, G.H. and CORBIN, K.W., 1978. Genetic variation and differentiation in the Parulidae. Auk, 95:691-702. BUIS, R.C. & TUCKER, E.M., 1983. Relationships between rare breeds of sheep in the Netherlands as based on blood-typing. Animal Blood Groups Biochemical Genetics 14, 17-26. CAMPBELL, Q., 1995. The indigenous sheep and goat breeds of South Africa. Dreyer Printers and Publishers. CLARKE, S.W., TUCKER, E.M. & OSTERHOFF, D.R., 1989. - Blood-groups and biochemical polymorphism in the Namaqua sheep breed. Animal Genetics 20, 279- 286. CORBIN, K.W., SIBLEY, C.G., FERGUSON, A., WINSTON, A.C., BRUSH, A.H. and AHLQLTIST, J.E., 1974. Genetic polymorphism in New Guinea starlings of the genus Aplonis. Condor 76, 307-318. CORBIN, K.W., 1983. Genetic structure of avian systematics. Curr. Ornith. 1, 211-244. EVANS, P.G.H., 1987. Electrophoretic variability of gene products. (Edited by Cook, F. and Buckley, P.A.) Academic Press, London, pp. 100-160. FERREIRA, IT., GRANT, W.S. and AVTALION, R.P., 1984. Workshop of fish genetics. CSIR special publication, Pretoria, p. 72. GRANT, W.S., 1989. Workshop of animal protein electrophoresis. University of the Witwatersrand, Johannesburg, pp. 1-124. HARRIS, H. & HOPKINSON, D.A., 1976. Handbook of enzyme electrophoresis in human genetics. North Holland Publising Company, Amsterdam. American Elsevier Publishing Company, Inc. New York. HASSELHOLT, M., 1969. Serum protein polymorphism's in swine electrophoretic identification. Genetics and Application. Munksgaard, Copenhagen pp. 48-52.
4- 1 MAN-WELL, C., BAKER, C.M.A. and CHILDERS, W., 1963. The genetics of haemoglobin in hybrids - I: A molecular basis for hybrid vigor. Comp.. Biochem. Physiol. 10, 103-120. MANWELL C. & BAKER, C.M.A., 1977. Genetic distances between the Australian Merino and the Poll Dorset sheep. Genetical Research 29, 239-244. MARKET, C.L. & FAULHABER, I., 1965. Lactate dehydrogenase isozyme patterns of fish. J. Exp. Biol. 159, 319-332. MATSON, R.H., 1984. Application of electrophoretic data. Auk 101, 717-729. NEI, M., 1972. Genetic distance between populations. The American Naturalist 106 (949), 283-292. NEI, M., 1976. Mathematical models of speciation and genetic distance. In: Population Genetics and Ecology (ed. S. Karlin and E. Nevo) pp. 723-766. New York: Academic Press. NEI, M., 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89, 583-590. NEI, M., 1986. Defmition and-estimation of fixation indices. Evolution 40, 643-645. NGUYEN, T.C., MORERA, L., LLANES, D., & LEGER, P., 1992. Sheep blood polymorphism and genetic divergence between French Rambouillet and Spanish Merino: role of genetic drift. Animal Genetics 23, 325-332. ORDAS, J.G. & SAN PRIMITIVO, F., 1983. A new allele at the red cell carbonic' anhydrase locus in sheep. Animal Blood Groups and Biochemical Genetics 14, 233- 234. ORDAS, J.G. & SAN PRIMITIVO, F., 1986. Genetic variation in blood proteins within and between Spanish dairy sheep breeds. Animal Genetics 17, 255-266. OTA, T., 1993. DISPAN. Pennsylvania State University, Philadelphia. RAYMOND, M. & ROUSSET, F., 1995. GENEPOP (version 3.1b): Population ge- netics software for exact tests and ecumenicism. J. Heredity, 86, 248-249. RLDGEWAY, G., SHERBOURNE, S.W. & LEWIS, R.D., 1970. Polymorphism in the esterases of Atlantic herring. Trans. Am. Fish. Soc. 99, 147-151. SELANDER, R.K. & KAUFMAN, D.W., 1973. Genetic variability and strategies of adaptation in animals. Proceedings of the National Academy of Science USA 70-76, 1875-1877. SHAW, C.R. & PRASAD, R., 1970. Starch Gel Electrophoresis of Enzymes - a Compilation of Recipes. Biochemical Genetics 4, 297-320.
4 - 2 STOKER, D.J., 1977. Statistical Tables, 3rd ed. H and R Academia (Pty.) Ltd., Pretoria, p. 35. STORMONT, C., SUZUKI, Y., BRADFORD, B.E. & KING, P., 1968. A survey of hemoglobin's, transferrins and certain red cell antigens" in nine breeds of sheep. Genetics 60, 363-371. SWOFFORD, D.L. & SELANDER, R.B., 1981. BIOSYS 1:aFORTRAN program for the comprehensive analysis of electrophoretic data in population genetics and systematics. Journal of Heredity 72, 281-283. TERBLANCHE, E., 1978. Ken ons kleinvee rasse. Edt. Human en Roussou uitgewers (Edms.) Bpk. Press. Nationale Boekdrukkery, Goodwood. VAN ZEVEREN, A., 1995. A genetic study of four Belgian pig populations by means of seven microsatillite loci. Pig workshop abstracts. International Society for Animal Genetics pp.14. WILLIAMS, J.G.K., KUBEL1K, A.R., LIVAK, K.J., RAFALSKI, J.A. and TINGEY, S.V., 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Reseach., Vol. 18: NoT22T6531-6535. • WRIGHT, S., 1978. Evolution and Genetics of Populations, Vol 4. Variability within and among Natural Populations. University of Chicago, Chicago. WUHLAND, S., 1928. The combination of populations and the appearance of correlation examined from the stand point of the study of heredity. Hereditas 11, 65- 106.
4- 3 .!" "GENEPOP INPUT FILE FOR. 19 SHEEP BREEDS" TF, ALB, CA, X, HB, pop • 0001 DOR,' 0204 0202 0202 0202 0202 0102 0021 RRP, 01030202 02020202 0102 0102' • 0002 . DOR, 0204.0202 0202'0202 0202 -0101 • 0022 RRP„. 0205 0202 0202 0101 0202 0202 0003 ,DOR, 04040202 0202 0202 0202 0202 • 0023 'RRP,. '0202 0202 0202'0202 0202 0202. . :0004 • DOR,- 0204 0202 0000 0000.0000 0000. 0024 RRP, 0203 0202 0202 0202 0102.0202 0005 DOR, _04040202 0202 0202 0202 0101 0025 RRP, 0103 0202 0202 0202 0202 0202 - 0006 DOR, 0404 0202 0202 0202 0202 0101 0026 :RRP, 0505 0202 0202 0101 0202 0102 0007 . DOR; 0204 0202 0202 0202 0202 0101 0027 .RRP, 0103 0202 0202.0202 0202 0101 0008 DOR,.. 0102 0202 0202 0202 0202 0101 1 . 0,028. -RRP, 0305 0202 0202 0202 0202 0102 . 0009 .DOR, 0204.0202 0202.0202 -0202 0102- 0029 RRP, 0405 0202 0202 0202 0102 0102 0010. DOR,. 0204 0202 0000 0000 0000 0000. 0030' RRP, 01020202 0202'0202 0102 0102 0011 DOR, 0404 0202 0000 0000 0202 0000 0031 RRP, .0305 0202 0202 0202 0202 0101 0012 DOR, 0304 0202 0202 0202 0202 0101 0032 RRP, 0102 0202 0202 0202. 0102 0102 0013 DOR, 010.1 0202 0202 0202 0102 0202 0033 RRP, .0404 0202 0202 0101 0202.0102- 0014 DOR, 0204' 0202 0000 0000 00000000 . 0034 RRP, 02030202 -0202 0202 02020101 . • 0015- DOR, 0204'0202 0202 0202 0202 0102 0035 -RRP, 0104 0202 0202 0202 0102 0102. 0016 DOR, 0104'0202 0202 0202 0202 0101 • . 0036 RRP, 0405 0202.0202 0202 0102 0102 ... .0017. DOR, 0404 -0202 0202 0202.0102 0102 .. . 0037: RRP, 0305 0202 0202 0202 0102 0102 0018 DOR,. 0304,0202 0202 0202 0202 0101. , 0038 RRP, 0204 0202 0202 0202-0202 0101 0019 DOR,, 0404 0202 0202 0202.0202 -0101 0039. RRP; ..0202 0.202.0202.0202.0102 0202 0020 -DOR,* . 0204 0202 . 0202. 0101'0202 0101 0040 . RRP, . :0304 0202 0202 0101 9102 . 0202 0021 DOR,: 01020202 0000 . 0000 0000 0000 .. 0041 --.RRP;..: 0204 0202 0203.02020102.0202 :0022- DOR, 0404 0202 0202 0202 0202 0102. 0042. -RRP;. 0304 0202 0203 0202 0102'0102 0023. DOR, 0204 0202 0202 0202 0202 0101-. 0043 RRP,: . 0104'0202 0202 0101 0202 0101 .- . 0024 .:DOR, 02020202:0202 0202-0202. . 0044' RRP, .•0102 02020202 0101 0102 0101 0025. DOR,, ....0304 0202 9292.9202 . 0202 .0102 0045 RRP,. '0205 .0202 0202 0101 0202 0102 .. 0026 DOR, ...0104 0202 0202 0101 0202 0102 0046 -iRRP,•: 0204 0202 0202 0202 0202 0102 0027 DOR, ::0104 02020202 0202 0202 0101' 0047 .RRP;: .10303.0202 0202 0101 . 0202 0102 0028 DOR,..:.0.104. 02020000 0000 0000 0000 0048 RRP, 0203 0202 0202 0202 0202,0101 0029. DOR, --'0104 0202 0202 0101 0202 0101 • 0049' .. RRP, .0204 0202'0202 0202 0102 0102 0030 DOR,•••• *0204. 0202.0202 0202 0202 0102„ 0050 •RRP, - 0405 0202.0202 0101 0102.-0102 . ...0101, 0202 0202. 0202 02020101 ... 0051 RRP, 0205 0202 0202 0101 0202 0102 *.• . 0032. DOR, ' 04040202 0000 pogo 0000 0000 -0052 • RRP; 02020202 0202 0202 01020.101 . .. 0033 DOR,' ;.0104:0202 0202.0202 0102 0101 . .0053 RRP, • 0202.0202 0202 0101 0102 0202 0034 .-DOR, • :0404 02020202 0202 0102 0202 0054. RRP, 0205 . 0202 0202 pot 0202 0202: 0035 DOR, ...0204 0202 0202.0202 0102 . 0101* 0055 'RRP; 0203 0202 -0202 0101 0102 0101 - 0036 DOR, _0101 0202 0000.0000 0000 .0000 0056' .RRP; 0203 0202 0202 01010202 0102 0037 _ DOR,- .:0204 .0202.0000 0000 0000 0000.. 0057' RRP', .0202 0202.9202 , 0202'0202 0102 0038, DOR, 0101 02020000.0000 0000 0000. 0058, 0205 0202 0202. 0101 0202 0202 '0039 .D011; 0104 0202 0202 0101 0202 0102. 0059 RRP, 0205 0202,0202 0202 0202 0101 .0040 DOR, 0101 0202 000000000000 0000 ... 0060 -.RRP; . 0204.0202 0202.0202 0102 0101. 0041 . DOR, 0404..0202 00000000 0000 0000 0061 RRP,. 0202 0202.0202 0101 0202 0102
.0042_ DOR, 0104 0202 0000 0000 0101.0101 - 0062 RRP, 0205 0202 0202 0101 0202 0202 ;* • .0043 DOR; 0.104 0202 0000 0000 0000 0000* . 0063 RRP, 0203 0202 0202 0202 0101 0202 . 0044. DOR, 0102 0202 0202 0101:0202 0101- 0064 .RRP,, 0205,0202 0202 0202 .0202 0202 0045 DOR, 0404 0202 0202 0202 0202 0101 • 0065 RRP; 0102 0202 0202 0202 0102 0101 .. 0046 :DOR, 0404.0202 0202 0101 0202 -0102: _ 0066: RRP, • 0103 0202 020202020102.0101. 0047 DOR, 0104 0202 0000 0000 0000 0000 : 0067...RRP, 0202 0202 0202 0202 0202 0102 • 0048, DOR, 04040202 . 0202'0202 0202 0.102 0068 RRP, '0205-0202.0202-0202.0202 0101 0049 -DOR,: 0102 0202:0202.0202'0202 0101 0069. RRP, 0105 0202 0202: 0202 0202:0102_ 0050. DOR': 0204 0202 0202 0202.0202 0101 0070 RRP, '0204 -.0202 0202 0202 0202 0102 0051 DOR, 0204.0202 0202 0202.0202 0101' 0071 RRP,. .0102 0202 02020101 0202 0102 . 0052' DOR, 0202 02020202.0202 0202.0101... 0072 RRP, 0304..0202 0202 0202 0202 0202. 0053 DOR, 0102 0202 0202 0.101 0202:0202 • 0073 RRP, . 0202 0202 0202 0101 0202.0102- 0054 -DOR; 0204.0202 0202 0101 0102 0101. 0074,-,RRP,. 0102 0202 0202 0202 0102 0102. 0055.. DOR, 0102 0202.0202,0101 0202 -0101*. 0075 .RRP, 0105.0202 02020202 0102 0101.. . 0056 •DOR, '0404. 0202 0202 0101 0202 0101 0076. RRP, 0101 0202 0202 0202 0102 0101 . ..0057 DOR, 0102 0202 02020101.0202 0102'. 0077 RRP, 0405 0202 . 02020202 0102 0102 . 0058 DOR, 0304 0202 02020202 0202 0101 0078 RRP, 0204 0202 0202 0202 0202 0202 0059 DOR, .0104 0202 0000.0000.0000 0000 0079 RRP, 0405 0202 0202 0202 0202 0101 0060 DOR, 0204 0202 0202 0202: 0102 0102 -- 0080 RRP, 0105 0202 0202 0202 0102 0101 0061 DOR, 0404 0202 0202 0202 0202 0102- 0081 RRP, 0203 0202 0202 0202 0202 0062 DOR, 0404 0202 0202 0202 0102 0101 01020082 RRP, 0105 0202 0202 0202 0102 0063 DOR, 0102 0202 0000 0000 0202 0101 0102 0064 DOR, 0404 0202 0202 0202 0102 0101 0083 RRP, 0202 0202 0202 0101 0202 0101 0065 DOR, 0204 0202 0202 0202 0202.0101 .• 0084 RRP, 0405 0202 0202 0101 0202 0202 0066 DOR, 0204 0202 0202 0202 0102 0101 0085 RRP, :0204 0202 0202 0101 0202 0101 0067 DOR, 0101 0202 0000 0000 0000 0000, 0086 RRP, 0204 0202 0202 0202 0202 0202
0068 DOR, 0404 0202 0202 0202.0102 0101 • 0087 RRP, : 0202 0202 0202 0202 0101 0101 0069 DOR, 0404 0202 0202 0202 0202 0101 0088 RRP, 0405 0202 0202 0202 0102 0202 0070 DOR, 0404 0202.0202 0202 0102 0101 0089 RRP, 0104 0202 0202 0202 0101 0102 0071 DOR, 0204 0202 0202.0202 0202 0101 0090 RRP, 0104 0202 0202 0202 0101 0101 0072 DOR, 0304 02020202 0202 0202 0202. 0091 RRP, .0102 0202 0202 0101 0202 0102
0073 DOR, 0204 02020202 0202 0102 0101 . 1 0092 RRP, 0104 0202 0202 0101 0102 0101 0074 DOR, 0102.02020202 0202 0202 0101. 0093 RRP, 0202 0202 0202 0202 0102 0102 0075 DOR, 0204.0202.0202 0202 . 0202'0101 .• 0094 RRP, 0104 0202 0202 0202 0202 0102 0076: DOR, 0404 0202. 0202 0202 0202 0202 0095 RRP, 0505 0202 0202 0202 0102 0101 0077 DOR, 0204 0202'0202 0202 0202 0102 0096 RRP, 0304 0202 0202 0101 0202 0102 0078 DOR, 0104.0202 0202 0202 0202 0102'2- 0097 RRP, 0405 0202 0203 0202 0202 0202 0079 DOR, 0204.0202 0202 0101.0202 0101, 0098 RRP, 0104 0202 0202 0202 0102 0102 0080 DOR, 0204 0202'0202 0101 0202 0101 0099 RRP, 0202 0202 0203 0202 0101 0101 0081 DOR, .0404 0202 0202-0101 0202 0202:- 0100 RRP, 0205 0202 0202 0101 0101 0101 0082 DOR, 020402020202 0202 0202 0202 -2 0101 RRP, 0204 0202 0203 0202 0102 0102 0083 DOR, 0404 0202 0202 0202.0202 010 1 . • 0102 RRP, 0204 0202 0202 0202 0202 0202 0084 DOR, 0.104 0202 0202 0202 0202 0101 0103 RRP, 0204 0202 0202 0202 0202 0202 0085 DOR, .0404. 0202 0000:0000.0000 0000 0104 RRP, 0203 0202 0203 0202 0102 0102 0086 DOR, 0404 0202 0202 0202 0202 0101..: 0105 RRP, 0205 0202 0202 0202 0202 0102 0087 DOR, 0102 0202 0000 0000-0000.0000 POP 0088 DOR, 0204 0202 00000000 0000 0000. 0001 BLE, 0101 0202 0202 0202 0102 0101 0089 DOR, 0204 0202 0202 0202 .02020101: 0002 BLE, 0102 0202 0202 0202 0202 0101 0090 DOR, 0404 0202 0202 0202 0202 0101 .. 0003 BLE, 0204 0202 0202 0101 0102 0101 0091 DOR, 0204.0202 0202 0202 0102.0102 0004 BLE, 0203 0202 0202 0202 0202 0102 0092 DOR, 0102 0202 0000 0000 0000 00002: 0005 BLE, 0104 0202 0202 0202 0202 0101 0093 DOR, 0104 0202 0202 0202.0202 .9101 . 0006 BLE, 0102 0202 0202 0202 0202 0202 0094 DOR, 0104 0202 0202 0202. 0202 0101 .. 0007 BLE, 0102 0202 0202 0202 0202 0101 0095 DOR, .0104.0202 0202 0202 .01020101', 0008 BLE, 0202 0202 0202 0202 0202 0101 0096 DOR, 0101.0202 0202 0101 02020101 0009 BLE, 0202 0202 0202 0202 0202 0102 0097 DOR, .:0204 0202.0202'0202 0202 0102' 0010 BLE, 0104 0202 0202 0202 0202 0101 0098 DOR, 0204 0202 0202 0202.0202 0102. 0011 BLE, 0104 0202 0202 0202 0102 0102 0099 DOR, 0404.0202 0000 0000 0000 0000, • 0012 BLE, 0102 0202 0202 0202 0202 0202 0100 DOR, 0204 0202 0000 0000 0000 0000 0013 BLE, 0404 0202 0202 0101 0102 0101 0101 DOR, 0104. 0202.0202 0202:0202 0101 0014 BLE, 0304 0202 0202 0202 0202 0101 0102 DOR, 0404 0202'0202 0202 0102 0101 0015 BLE, 0104 0202 0000 0000 0000 0000 0103 DOR, 0304 0202 0202 0202 0202 0202. 0016 BLE, 0303 0202 0202 0202 0202 0101 0001; WDO, .0404:0202 0202 0202 0202 -0101- 0017 BLE, 0204 0202 0202 0202.0202 0101 0002 WDO, 0104 0202 0000 0000 0000 0000. 0018 BLE, 0202 0202 0202 0202 0202 0101 0003 WDO, 0204.0202.0202 0202.0202 0.101. 0019 BLE, 0204 0202 0202 0202 0202 0101 0004 WDO, . 0404 0202 0202 0101 0202 0102 0020 BLE, 0204 0202 0202 0202 0202 0101 0006 WDO, . 0204 0202.0000:0000 0000 0000, 0021 BLE, 0204 0202 0202 0202 0202 0101 0007 WDO, 0204.0202 0202 -0202.01020101 0022 BLE, 0102 0202 0203 0202 0202 0101 0008 WDO, 0000 0000 0202 0202 0202 0101 0023 BLE, 0204 0202 0202 0202 0202 0101 0009 WDO, 0304 0202 0000 0000 0000 0000 0024 BLE, 0202 0202 0202 0202 0202 0101 0010 WDO, 0202 0202 0202 0202 0202 0101. 0025 BLE, 0102 0202 0202 0202 0202 0101 0011 WDO, 0103.0202 0000 0000 0000 0000 0026 BLE, 0102 0202 0202 0202 0202 0101 0012 WDO, 0404 0202 0202'0202 0202 P101.: 0027 BLE; 0101 0202 0202 0202 0202 0102 0013 WDO, 0102 0202 02020202 -0202.0202 • 0028 BLE, 0303 0202 0202 0202 0202 0102 0014 WDO, 0304 0202.0202 0202 02020101 : 0029 BLE, 0304 0202 0202 0202 0102 0101 0015 WDO, 0304 0202 02020202 0102 0101 0030 BLE, 0102 0202 0202 0202 0202 0101 0016 WDO, 0204 0202 0202 0202 0202 0101 0031 BLE, 0202 0202 0202 0202. 0202 0101 0017 WDO, 0204 0202 0202 0202 0202 0101 0032 rBLE, 0304 0202 0202 0202 0202 0102 0018 WDO, 0304 0202 0202 0202 0202 0101 0033 BLE,. '0103 0202 0202 0202 .0202 0101 • 0019 WDO, 0202 0202 0202 0202 0202 0101 0034 BLE, '0303.0202 0202:0202 0202 0202. 0020 WDO, 0202 0202 0202 0202 0202 0102 0035 BLE,• 0102 0202 0202 0202 0202 0101 PoP • 0036 BLE, • .0103 0202 0202 0202 0202 0101 0001 . PED, 0404 0202 0202 0202.0202 0102. . 0037 .BLE,.. 0202 0202 0202 0202 0202 0101 0002 PET), 0404'0202 0202 0202 0202 0102 . 0038 BLE, 0202 0202 0202 -0202 0202 0101 .0003. PED, 0404 0202 0202 0202 0202 0102 0039 BLE, 0102 0202.0202 0202 0101 0101 • 0004 PED, .0104 0202 0202 0101 0202 0101 , 0040 . BLE,• •..0102 0202 0202 0202 0202 0101 0005 PED, 0404 0202 0202.0202 0202 0101 ... 0041 BLE, 0304 0202 0202 0202'0202- 0101 0006 PED, 0404 0202 0202 0202 0202 0102 0042 BLE; 0304 0202 0202 0202 0102 0101 0007: PED, 04040202 0202,02020202 0102 0043 BLE, 0203 0202 0202 0101 0202 0101. 0008 ' PED,. 0404 0202- 0202 '0202 . 0202 '0102 0044 BLE, 0101 0202 0202_0202 0202 0101 0009 PED, 0404 0202 0202 0101 0202 0101 0045. BLE, - 9203 0202 0202 0202 0202 0101 0010 PED, 0404.0202 0202'0202 0202 0102. • 0046. BLE, • 0303 0202 0202 02020202 0101 0011 . PED;• 0404 0202 0202 0202 0202 0101. *. 0047 BLE, - 0303 0202 .0202 0202 0202 0101
0012 PED , *9404 0202 0202 0202 0202 0202 0048 - BLE, -0203 0202 0202 0202 0202..0102- 0013 PEI); 0304.0202 0202 0202:0202 0102 0049 BLE, ':. 0101 0202 0202 0202 0102 0101 0014 PED; 0404.0202 0202 0202 0202 0102 0050 :BLE; • 0103 0202 0202 0202 0202 0101. 0015 FED,' 0404'0202 0202 0202 0202 0202 -.: * 0051 • BLE, 0205 0202 0202 0202 0202 0102 0016 ...PED; 0404 0202 0202 0202.0202 0101• 0052 . BLE ;;• '0102 0202 02020202 0202 0101 .. 0017 13ED;, 0304 02020202 01010202 0102• : :* 0053 'BLE, .0203,0202 0000 0000 0000.0000 0018. PED; 0404 0202 0202'0202 0202:0102 , . 0054. mg,.. 0303 0202.0202 0202.0202 0102 • :.0019 FED,. 0404 02020202 0202 0202 0102 • .. 0055 BLE, 0103 0202 0202 0202 0202 0102 0020... PED; 0104 0202.0202 0101 02020101 y 0056 BLE, '0702.0202 0202 0202 0202 .0101 4021 PED, 0404 0202 0202 .0202 0202 0102: • 0057 BLE,.•0103 0202 0202 0202 0202 0101 •. _0022__PED, 0404.0202 0202 0202 0202 0102. 0058 -BLE,_L0205 0202 0202 0202.4202 0102 •• 0023 PED, :0404 0202 0202.0202 0202 0202 0059. BLE, :02020202 0202 0202 0202 0102 • : 0024 'FED, 0404.0202 0202 0202 0202 0101..:. 0060 BLE, . 0202 0202 0000 0000 0000 .0000.. : 0025. PED, 0404 0202 020/0202 0202 0 .102- 006113LE, 0102 0202. 0000 0000 0000 0000 ...0026 PED 0404 0202-0202 0202 0202 0202, • . 0062 . BLE;.. 0104'0202 0000. 00000000. 0000 : 0027. :PED, .0404 0202.0202 0202 0202 0202 .- 0063 BLE, •-• 0304..0202 0000 0000 0000 0000
0028 •PED,. -0303 0202 0202.0202 .0202 0.102 0064' BLE,‘ 0404 0202.0000.0000 0000 0000 • 0029 PED;*. -0304 0202 0000 .0000.0000 0000 0065 BLE,: 0102 0202.0000 0000.0000 0000
• 0030 PED, 0304.0202.0202 0101 0202 0 . 101 .: .0066 :• BLE, 0202 6202 , 0000 0000 0000. 0000 • 0031 :PED, 0104 0202 0202 02020202 0102 0067 • BLE, • 0104 0202 ,000000000000 .0000 - 0032 • PED, 0304.0202,0202 02020202:0102.. .. 0068. BLE, .0202,0202 0000 0000 0000 0000 0031 FED;. '.0404 0202 0202 0202 0202 0101 : : 0069 BLE, * 0102 0202 0000 0000,0000 0000.
. 0034 -PEW 0404.0202 0202 0101 0202 0202 . ; - 0070 BLE, 0203 -0202 0000 0000 0000 0000: . 0035 PED, 0404 0202 0202 020202020202'. 0071 . BLE, 0204.0202 0000 0040:00000000 ..0036.-PED, 0404 02020202 0202 0202.0101 •••• POP- 0037. PED; 0404 9202: 0202 . 0101..0202 0101 0001: SKP;'.. 0104 0202 0202 0101 02020101 . 0038 PED,. 0404 0202 0202 0101.0202 0202 0002. SKP,. 0404 0202.0202 0101 0202 0101 • 0039 .PED; 0404 0202 0202 0101 0202 0102 0003 SKI', 0104 0202 0202 01.010202:0101 . 0040 PED, 0404 0202 0202 0202 0202 0102.. 0004, SKP, • 0101 0202 0202 0202 0202 0202. 0041 • PEP,: 0304 0202 0202 0202.0202 0102'. 0005. SKP, • 0404 0202 0202 0101 0202 0102 0042 PED, • 0104 0202 0202 0202 0202 0101 . 0006_ 'SKI', . .0404.0202 0202 0101 0202 0101 0043 PED,, 0404 0202 0202 0202 0202 .0102 0007 SKP,. 0404 0202 0202 0101 0102 0202. 0044 PED, 0404 . 0202 0202'0202 0202 0102 0008 SKP, 0104 02020202 0202 .02029202 0045*:PED, 0404 0202 0202 0202 0202,0202 0009. - MCP,. 0104 0202 0202 0202' 0202 0202
0046 PED; 03040202- 0202 . 0202 0202'0101. .- 0010. SKP; 0104 0202 0202 0202 0202.0202 :- 0047 PED. 0404 0202 92020202 02020102: 0011 SKP,. 0104 9202 0202'0101 0202 0202 0048 PED; 0304,0202 0202 0202 0202,0202 • 0012 SKP, '.0404 0202 0202 0101..0202 0102'. .0049 • PEW 0404 0202 0202 0202 0202 0102 *. :0013 •SKP,- 0303 0202 0202 0202 0202 0102 0050 .PED, 0404.0202 0202 0202 0202 0102 . ... 0014 - SKI); , :0204 0202 02020101.0202 0101 ...0051..PED, 0304 0202.0202'0101 9202 0101 •• • 0015 -SKP; *0404 0202 0202 0202 02020101 0052 PED; 0404 02020202 0202.0202.0101 . . 0016 .SKP;- .0404.0202 0202 0202 0202 0101 * 0053 FED; 0404 0202 0202. 0202 0202 0202 :*. 0017 SKP;* 0404 0202 0000 0000:0000.0000 0054 'FED,' 03040202'0202 0202 .-0202 0101' 0018 SKP, 0104 0202 0202 0202 0202 0101 • 0055 PED, 0404 0202 0202.0202.0202 0202 • 0019 SKP„ 0404 0202 02020202 0202 0101 0056- PED„ 0404 0202.0202 .0101 0202 .0102 0020. SKP; 0404 0202,0202 0202:0202.0102 • 0057 PED, • 0404 0202 0202 0202 0202 0102 . . 0021 SKP, 0204 0202 0202 0202 0202 0202 0058 PED,. 0404 0202 0202 0202 0202 0102 _. 0022 SKP, 0404 0202 0202 0202 0202 0101 0059 PED, • 0404'0202 0202.0202 0202 0202 0023 SKP; 0404 0202 0202 0202 0202 0.102 0060. PED, • 0304-02020202 0202. 0202 0102 0024 . SKP; 0404 02020202'0202 0202 0101 0061- PED; - 0303 0202 0202 0202 0202.0102 0025 SKP, 0103 0202 0202 0202 0202 0101. 0062 PED, 0404 0202 0202 .0202 0202.0102 . 0026 SKP, 0404 0202 0202 0101 0202 0101 0063. PED, 0404 0202 0202 0202 0202 0101 0027 SKP, 0104 0202 0202 0101 0202 0101- 0064 PED, . 0304 0202 0202 0202 02020202. • 0028 SKP, 0104. 0202 0202 0101 0202 .0101 0065 PED,. 0104 0202 02020202.0202 0101 . 0029. SKP,- 0104 . 0202 0000 0000 0000 0000. 0066 PED„: 0404 0202 0202 0202 0202 0101 0030 SKP, 0204 0202 0000 0000 0000 0000 . 0067 PEW 0304 0202 0202 0202 0202 0101. 0031 SKP, 0404 0202 0000 0000_0202 0101 .
- 0068 PED, 0404 0202 0202 0202 0202.0101 0032 SKP, 0404. 0202 0000 0000 0000 0000 0069 PED, 0404 0202 0202 0202 0202 0102 . 0033 SKP, 0000 0000 0000 0000 0202,0000 . . 0070 PED, 0404'0202.0202 0202 0202'0202 . .0034 SKP; 0404 0202 0202 0101 0202 0101 0071 . PED, '203040202 0202 0202 -0202 0202 0035 SKP, 0104.0202 0000 0000 0000 0000 0072 PED, 0404 0202 0202 020202020101 0036 SKP;' 0104 0202 0000 0000 0202 0000: 0073 PED, :0404 0202 .0202,010102020102. . 0037 SKP, 0101 0202 0202 0101 0202 0101-.. 0074 PED, 0304 0202 02020000 0202 0000. -0038 SKP, 0404 0202 00000000 0000 -0000 - . 0075 0404 0202 0202 0000 .0202 0000 0039. 04040202 0202 0202 0202.0101
0076 0404 0202 0202 0101 .0202,0102 . . . 0040 . SKP 0104 .0202.0202 0101 0202 0102 0077.. PED,. 0404 0202 0202 0101 0202 0101 0041. _SKP; . 0404 0202 0202 0101 0202 0101' :0078 PED,. • 0104 0202.0202 0000 0202 0000 .0042., SKP, -.02040202 0202 0101 0202 0101 • 0079. PED, :•.040402020202 . 0101 0202 0102 .. 0043: SKP,• 0102 0202 0202 0202 0202 0101 0080 PED;. '0404 0202.0202 00000202 0000 0044 SKP„ 0104.0202 0202 0101 0202 0101 0081 TED, : .0404 02020202 0000. 0202. 0000 0045. SKP,.• 0404 0202 .0202 0101:0202 01.01 .0082-PED, L0304 0202 0202'0000 .0202 0000 • 0046 ,SKP, :0104 02020202 0202 0202 .01.01 0083 PED; .0404. 0202 0202 0000: 0202:0000 0047 ; SKP; 0104;0202 0000 0000 0202 0000, 0084 PED,. 0404 0202 0202 0000 0202 0000..•-, 0048 SKP; 0404 0202 0202 0101 0202'0101 0085 :PED, .0404,0202 0202 0000 0202 0000 .. 0404 0202.0202:0202 0202 0102' .0086 PED,• 0404 0202 0202 00000202 0000 0050 SKP, 0204.02020000 .0000 0000 .0000... 0087 PED, • 0304 0202 0202_0000 0202 0000 0051, SICP,. 0101 .0202.0202 0202 0202 . 0101 .0088 .PED, -.0304 0202 0202 02020202.0101 • -• 0052 SKP, 0304 0202 0202 0101.0202 0101.: • .0089. PED; • 0404 02020202 0000.0202,0000 0053., SKP,. 0000 0000 0202 0101 0202 0102 . 0090 PED, .04040202 0202 0000.0202. 0000 0054 . SKP, 0104 0202.0202 0202.0202 0101 0091• PED; . 0404 .02020202-0000 .0202 0000 , 0055 . SKP, 0404 0202' 0202 0101 0202•0101 . 0092 • PED; . 0404. 0202-0202 0000.0202 0000 0056 SKP; 0104 0202 0202 0101'0202 0101 .. 0093-:PED,''. 0404 0202 0202 0.000 0202.0000 • 0057 . SKP,• 0404 0202 0202 01010202.0101, • 0094 PED; • 0404 0202 0202 0000 0202 0000 0058,'SKP, 0102.0202.0202 0202 0202 0101 • 0095 PED, 0404.0202 0202.0202 02020101. 0059. SKP, 0404, 0202 0202 0202 0202 0102 ••. 0096 .PED; 0304 0202 020202020202.0202 0060 SKP,. 0404 0202 0202 0202 0000 0101. - 0097 'PED,..:0404 0202 0202 0000 0202 0000 0061 - SKP; 0404 0202 0202 0202 0202 0102 0098-PED;••• 0404 0202 0202 0202 02020101. 0062 SKP,- 0404 0202 02020101 0202 0101 . . 0099 PED; 0404 0202 0202 0000 0202 0000. 0063. :KU, : 0404 0202 0000 0000 0202 0000. 0100., PED ;. . 0303 0202 0202 .02020202 .0101.- • 0064 •SKP;. 0404 0202 0202 0202 0202 0101 u306 , • 0404 0202 0202 0202 0202 0101 -0065 we; 0404 0202 .0202 0202 0202 0101-, R61 :..• -0404 0202'0202 0202 0202.0101 0066 SKP,. 0204 0202 0202. 0101- 0202.0101 • U289.•;: • . 0404 0202 0202 0202 0202 0101 . 0067.: SKP, 0204.0202.0202 0101.0202 0101 : W209-, • .• 0404 0202 02020202 0202 .0101 0068 SKP„ 0404.0202 0202 0101 0202 0101. .W198•,•: .•• • 0404,02020202 0202 0202,0102.. 0069 SKP, 0104 0202 0202 0101 0202 0101 T190 ; • 0404 0202 0202 02020202-0102 . 0070 . SKP, 0104 -02020202.0202.0202 0102 T171 , 0404 0202 0202 0202 0202 0102 • 0071' SKP, 0404 0202 0202 0202 0202 0101. .. U302 0202 0202 0202 0202 0101 .• 0072.. SKP; 0104.0202 0202 0202 0202 0121... W217 , , , .0404.0202 0202.02020202 0101 0073 . SKP,.. 0404 0202 0202 0101 0202 0101 885 , .0204 0202 0202 0202 0202 0101 . 0074.- SKP,. • 0104 0202 02020202.0202 0101.. . W197.; • ..0404 0202 0202 0202 0202 0102 0075 • SKP, 0404 0202 0202 0101 0202 0101 P48 :,•• 0404 .0202 •0202 0202 0202 0101 0076 SKP;,- ..01040202,0000 0000. 0000 0000 '• P72 ;..• • 0404 0202 0202 020202020102.: • 0077. SKP, ' , 0204 0202 .0202 0101 0202 0101- • R63 , 0204 0202 0202 0202 0202 0202 - 0078 • SKP; . 0204 0202 0000 0000 0000 0000
T193 , 0404 0202 0202 0101 0202 0102 0079 SKP, 0204 0202 0202 0101 0202 0101 W207 , 0404 0202 0202 0202 0202 0101 0080. SKP, '0404 0202 0202 0202 0202 0101 W208 , 0404 0202 0202 0202 0202 0101 0081 SKP, 01040202 0202 0101 0202 0102 W215, 0404 0202 0202 0202 0202 0102 0082 SKP,.- 0101 0202 0202 0101 0202 0101 T186 , 0404 0202 0202 0202 0202 0202 0083 SKP, .04040202 0202 0101 0202 0101 S77 ...;•••• 0404 0202 0202 0202 0202 0102 0084 -RCP, 0104 0202 0000 0000 0000 0000
T183 , 0404 0202 0202 0202 0202 0101 .- 0085 SKP, : 0404 0202 0202 0202 0202 0000 ,T172 , 0404 0202 0202 0202 0202 0101 . 0086 SKP, 0404 0202 0000 0000 0000 0000 W194 , .:0404 0202 0202 0202 0202 0102 : 0087. SICP, 0204 0202 0202 0101 0202 0101 W204 , ••• 0204 0202 0202 0202 0202 0202. 0088 .SKP, 0104 0202 0000 0000 0000 0000 S75 . '0404 0202 0202 0202 0202 0102 - 0089 SICP; • 0104 0202 0202 0101 0202 0101 W203 ; .0404 0202 0202 0202 0202 0101 0090. SKP, 0404 0202 0000 0000 0000 0000 U288 ,. 0404 0202 0202 0202 .0202 0102 • 0091 SKP, 0104 0202 0202 0101 0202 0101 S62 0204 0202.0202 0202 0202 0202 0092. SKP, 0404 0202 0202 0202 0202 0101 T192 ,• 0404 0202 0202 0202 0202 0101 • 0093 SICP, 0404 0202 0202 0101 0202 0102 11303 , • 04040202:0202 0202 0202 0102 0094 SKP, 0404 0202 0202 0101 0202 0202 '.T184 , . 0204 0202 0202 0202 0202 0202 0095 SKP, 0104 0202 0202 0202 0202 0102 W222 - 0404 0202 0202 0202-0201010.1 0096 SKP, 0102 0202 0202.0101 0202 0102 S70 , 0404 0202 0202 0202 0202 0102 0097 SKP,• 0404 0202 00000000 0000 0000 U296 ; 0404 0202.0202 0202 0202 0102 0098 SKP;. 0102 0202 0202 0202 0202 0101 1.1286i, 0404 0202.0202 02020202 0101. 0099 SKP, 0404 0202 0202.0202 0202 0101 T188 ; 0404 0202 0202 0202 0202 0101 0100 SKP, 0104 0202 0000 0000 0000 0000
.W228 .; 0404.0202 0202 0202 02020102 . . 0101'. SKP,- - 0404 0202 0202 0101 0202 0101 T176. • 0404 0202'0202 0202 0202 0102 0102 SKP, 0104 0202 0202 0202 0202 0101 S89 • ; ••: '0404 0202 -0202 0202 0202.0202 • 0103 S1CP, 0404 0202. 0202 0101 0202. 0,101 T175, .0204.0202 0202 0202.0202 . 0101: 0104 SKP, 0404 0202 0202 0101 0202.0102 U304 , 02020202 0202 0202 0202 0105 SKP, 0104 0202 0000 0000 0000.0000 W202 ;• ;0204 0202 0202 0202 0202 0102 .• • 0106 SKP, 0.101 0202 0202 02020202 0102 S66 0204 0202.0202.0202 0202 0107 • SKP, 0404 0202 0202 0202 0202 0101-- W225; 0404 0202 0202 0101 0202.0102 0108 SKP, , 0204 0202 0202 0202 0202 0101 U277 , 0404 0202 0202 .0101 0202 0202 .. 0109- SKP, • .0104 0202 0202 0101 0202 0101 W214 , . 04040202 0202 0101 0202 0102 .... . 0110 SKP,: 0104 0202 0202 0101 0202. 0202 890 .., 0404 0202..0202 0202 0202 .0102. 0111. SKP, 0404 0202 0202 0101 0202 0101 W196. , 0204 02020202 0202 0202 0101'. • 0112 SKP, 0104 0202 0202 0202 0202. 0101 -P62. 0404:0202.0202 0101 0202 0202 0113 SKP; 0104- 0202 0202 0101 0202 0101 T177 ; • 0404.0202 0202 0101 0202 0202 0114 SKP, 0204.0202 .0202 0101 0202 0101 .. W77. , 0404 0202 0202 0202-02020102 . 0115 SKP, 0104 0202'0202 0202 0202 0101 . A779 ..04040202 0202 0202,0202 0102. 0116 SKP, • 0404 0202 0000 -0000 0000 0000 .:: 0404 .0202 0202 0202 0202 0101 -.. . 0117 SKP, 04040202 0202 0202 0202 0101 :W64. 0404 0202 0202 0202. 0202 0102 . ..: 0118: SKP,. 0404 0202 0202 0101 0202 0101 . A792. ; 0204 0202 0202 0202 0202 0102. • • 0119 SKP, 0102 0202 0202 '0202 0202 000) . A773. , 0204 .0202 0202 .0202 0202 0202 . • 0120 SKP, 0404 0202 0202 0101 0202 0102. A783 0404 0202 0202.0202 00000202 0121 SKP, 0104. 0202 0202 0101 0202.010.1 A871 ;.. : :0404 0202 0202 0202 0202 0102 .. 0122 SKP, '0104 0202 0202 0101 0202 0101 1J428., 0204 0202 0202 0202 0202 0102 0123 SKP, 0104 0202 0000 0000 0000 0000 A875 , 0404 0202 0202 0202 0202: 0102 0124 SKP, 0404 0202 0202 0101 0202 0101 Pop- ,•. 0125 SKP, 0103 0202 0202 0202 0202 0202 : 0001 SAV, .0104.0202000.0 0000 0000 0000 • 0126 SKP, 0404 0202 0202 0202 0202 0102 0002 SAY, -0303_02020000 .0000 0000 .0000 0127 SKP,....0.101 0202 0202 0202 0202. 0101 :0003 • SAV, .0104 0202 0000 : 0000.0000 .0000: 0128 SKP, 0404 0202 0202 0202 0202 0202. 0004 SAY, ...0105 02029000 0000 00000000H :0129 SKP, 0204 0202 0202 0101 0202 0101 0005 •:• SAV., 0304 0202 0000_0000 00000000 0130 SKP, . -0404 0202 0202 0202 02020101. • .0006:.SAV, 0103.0202 0000 0000 0000.0000 0131 SKP, 0204.0202 0202 0202 0202 0101.. 0007•.SAV,; 0101..0202 0000 0000. 0000.0000 0132 SKP, '0104 0202 0202 0202 0202 0101 .0008 SAV,. .0303 0202 00000000 0000 0000 • .0133 . SKP, 0404 0202 0202 0202 0202 0102 0009 - SAV,- 0103 0202 0000 .0000 0000 0000 0134 SKP, 0.304 0202 0202 0202 0202 0101 0010 SAV, 0304 0202.00000000 mo0 woo -0135 SKP, .0404 0202 0202 0101 0202 0101 • 0011 SAV,• . 0101.02020000 0000 0000 0000.... 0136 SKP, 0404 0202 0202 0202 0102 0101 0012 SAV, 0101..0202 0000.0000 0000 0000 0137 .SKP, 0104 0202 0202 0202 0202 0101 0013: SAV, : 0104 0202 `0000 0000 0000 0000 0138 SKP, 0404 0202 0202 0202 0102 0101 0014 SAY, 0304 0202. 0000 .0000 0000 0000 0139 SKP, 0404 0202 0202 0202 0202 0102 0015 -SAV, _0405 02020000 0000 0000 0000 1-.0140 SKP, 0102 0202 0202 0202 0202 0101 0016 SAV, :0103 0202 0000 0000 0000 0000 .- 0141 SKP; 0404 0202 0202 0202 0202 0101 0017. sAy,• • 0305 0202 0000 0000 0000 .0000 • 0142 SKP, 0204 0202 0202 0202 0202 0101 0018 SAV, • 0103 0202 0000 0000 0000 0000. 0143 SKP, 0204 0202 0202 0101 0202 0102 0019 -SAV,-. :0101 0202 0000 0000 0000.0000- 0144 SKP, 0104 0202 0202 0202 0202 0101 .0020 SAY, . 0103. 0202 0000 0000 0000.0000 0145 SKP, 0404 0202 0202 0202 0202 0101 0021 :SAV, 0101 0202 0000 0000 0000 0000 0146 SKP, 0404 0202 0202:0101 0202 0101 .0022. -SAV, 0303 0202 0000 0000 0000 0000 .- 0147 SKP, 0404 0202 0202 0101 0202 0101 0023 .SAV, 0303 0202 0000.0000 0000 0000. 0148 SKP, 0404 0202 0202 0101 0202 0101 0024 SAV; .0104 0202'0000 0000.0000.0000 • 0149 SKP, 0104 0202 0000_0000 0202 0101 0025 SAVi -. 0104 0202 0000 0000 0000 0000 0150 SKP, 0404 0202 0202 0101 0202 0102 0026 SAV, 0303 0202 0000 0000 0000 0000 - 0151 SKP, 0404 0202 0202 0101 0202 0101 0027 SAY, 0101-0202 0000 0000 0000 0000. • 0152 SKP, 0104 0202 0202 0202 0202 0101 0028 SAY, 0104 0202 0000 0000. 0000 0000 0153 SKP, 0304 0202 0202 0202 0202 0101 ' '0029 ,SAV-; .0104 0202 0000 0000 0000 0000 0154 SKP, 0404 0202 0202 0202 0202 0102 0030. .SAV,. 1 : 0203 0262 0000'0000 0000 0000 0155 SKP, 0304 0202 0202 0202 0202 0101 0031 SAV„ :0303 .0202 0000 00000000. 0000 0156 SKP, 0304 0202 0202 0202 0202 0101 0032 SAV, • 0105-0202 0202 0202 0202 0202 0157 SKP, 0404 0202 0202 0101 0202 0102 0033 SAY, :0304 0202 0202 0202 0202 0102 • 0158 SKP, 0204 0202 0202 0202 0202 0101 0034,- SW 0105 0202'0202 0202.0202 -0202 0159 SKP, 0104 0202 0202 0202 0202 0101
0035 SAV, 0105 0202 0202 0202 . 0202 0202 0160 SKP, ' 0404 0202 0202 0202 0202 0101 0036. 0105 0202 0202 0202'0202 0102 0161 SKP, 0104 0202 0202 0202 0202 0101 0037. ..SAV 0405 0202 0202 0202 0.202.0102 0162 SKP, 0104 0202 0202 0202 0202 0101 0038 , SAV, ,..01040202 0202 0202 0202 0202 0163 SKP, 0104 0202 0202 0000 0102 0102 0039. SAY, - 0104 0202 0202 0202 0202 0202 . 0164 SKP, 0204 0202 0202 0202 0202 0101 0040: SAY, , '0105 02020202 0202 ,0202 0101 0165 SKP, 0204 0202 0202 0202 0202 0101 0041 . .SAV,.: 0105 .0202 0202 0202 0202 0202:: _0166 SICP,_0404 0202 0202 0101 0202 0102 0042 SAV, .0405 0202 0202.0202.0202 0000 0167 SKP, 0404 0202 0202 0202 0202 0202
0043 SAY, 0104 0202 02020202 0202 0202 0168 SKP, 0404 0202 0202 0202- . 0202 0101 0044. SAV, ..0101 0202 0202 0202 0202 0101 . 0169 .SKP;.- 0404. 0202 0000 0000 0000- 0000 0045 SAV,..' .0105 0202 0202 0202 0102 0101 . 0170 SKP,. 0104 0202 . 0202 0202 0202 0101 , 0046. SAY, .0205.0102 0202 0202 0202 0202 0171 SKP,.. 0404.0202 0202 0202.0202 0102.. 0047 SAY,' " 0105'0102 0202 0202 0202 0102 0172 . SKP, 0104 0202 0202.0202 0202 0101 . 0048 . SAY,-,; 0.1050102 0202 0202 0202-0102'- 0173 •SKP,: 0704.0202 02020202 0202 0102 0049 SAV, 0505 0202 0202,0202 0202 0102. 0174' SICP, 0204 0202 0000 0000 0000 0000. .0050 SAV;. 0405 0202 0202 0202 0202 0202 0175 ;SKP 0404 0202 0202 0101 0202 0202.
0051 SAV, . 0105 0202 0202 0101.0202 0102.. • 0176 SKP, 0102 0202 0202_ 0101 0202 0202' . -- 0052 SAY,. 0105'0202 02020202 0202 0101 '0177. SICP,. 0104 0202.0202_010.10202 0202: . _. 0053 .SAV; ,-;0105 0202.0202 .0101 0202 0202 .1 0178 SKP,. 0104,0202,0202 - 0202:0202 0101 • 0054 . SAV,: .--0105 0202 0202 0202 0000 0202'- 0179 0404 0202-0202 0101 0202 0101 . 0055 . SAY, ..0103 0202 0202 0202 02020101 0180 SKP, 0204.-02020202 0202 0202.0101... 0056 SAY,., ..0101 0202 0202-0202 . 0202 0202 0181 SKP, 0104. 0202 0202.0101.0202 0101-: • :0057 SAY, -0105 0202'0202 0202,0202 0101 0182 SKP;, 0104 0202 0202'0202. 02020101 0058.,.SAV, 0505 . 0202-0202 01.01 0202 0202 0183. SKP, 0404 020,2 0202 0202 0202 0102 0059: SAV, : 0103.0202 0202 02,02 02020102 0184 SKP, 0.1040202.0202 0202 0202 0101 .
0060. SAV; 0104 " 0202 0202 0202 0202'0202_ 0185 SKP, 020402020202 0101 0202 0202 0061 '-SAY,..0405 0202 0202 0202 0202 0102 .. • 0186 SKP; 0104 0202 0202 0101 0202 0202. 0062 SAV, 0104.0202 0202 0202 0202 0102 0187 SKP, 0404 0202 0202 0202 0202.0101 . 0063. SAW -..0101. 020.2 0202 0202 0202 0102 .. 0188 SKP, 0104 0202 0202 0202 0202 0101: .-0064 SAY, .0103 . 0202 0202 0202 0202 0101: ..0189 SKP,. 0404 0202 0202 0101 0202 0102 . 006.5 . SAY,- 0103 0202 0202 0202-0202 0102' . 0190 SKP, 0104 020202020202.0202 0102 0066 SAY, 0105 0202 0202 0202 - 0202 0101 0191 SKP, 0104 0202 0202 0202 0202 0101 . 0067 SAV 0101 0202 0202 0202 0202 .0202 .: 0192 - 0104 0202 0202 0202.0202 0101
0068 ' SAV; .0105 0202 0202 0202 0102 0102. 0193. SKP, 0102 .0202 0202 0202 0202 0102 0069 SAV, .0101 02020202 0101 0202 0101 . - 0194 SKP; 0104 0202 0202 02020202 0101-.' 0070 SAV; .0101 02020202 0202 0202 0102 0195 .S1CP,. 0404 0202 0202 0202 0202 0101' 0071, SAY, • 0104 0202 0202 0202 0202 0102 , 0196 .. SKP, .0404.0202.0202 01010202 0102.'. 0072. SAY, 0305 0202 0202 0202 0202 0101 0197 SKP;: 0404 0202 0202 0202. 0202.0101 . • 0073: SAV,: 0.101 0202 0202'0202 0202.0202 .. 0198' RCP; 0404 0202 0202 0101 0202 0101 0074 SAV, • 0101 .0202 02020101.0202 0102 0199 SKP, 0404 0202 0202.0101 0202 0102 0075 SAV, 0104 0202 0202 0202 0202 0202 0200 . SKR, - .010440202 0202 0101 0202 0101 0076 SAV, 0105 0202. 0202 0101 0202 0202 • 0201 SKP, 0404 0202 0202 01010202 0101 • 0077 SAY, 0101 0202'0202.0202'0202 0102 . 0202 SKP, 0404 0202 0202 0202 0202 0101 0078 SAV, 0105 0202 0202 0202 0202 0102 0203 SKP, 0204 0202 0202 0101 0102 0101 0079 SANT, 0105 0202 0202.0202 0102 0102 0204 :0104 0202 0202 0101 0202 0101 0080 SAV,. 0104 0202.0202 0101 0202 0102 .. . 0205 . SKP; '0104 0202 0202.0101 0202 0202 0081 SAV, 0101 0202 0202 0202 0202 0102 0206. 5EP,' '0404 0202 0202 0101.0202 0101
pop' • • . 0207 'SKP, 0204.0202.0202'0101 0202 0202 0001 LAN, .0104 0202 0202 0202 0202 0102 .. 0208 .SKP,... 0104 0202 0202 0101 0202 0101 0002 LAN, 0404 0202 0202 0101 0102 0102. 0209 .SKF';. : 0404 02020202 01010202 0101 0003: LAN, 04050 0202.0202. 0101 0102 0102 0210 SKP, ..0404 0202 0202 0101 0202 0101 0004..LAN, 0305-0202 02020202 0202 0102 . 0211 SKP, 0404 0202 0202 0101 0202 0101 0005 LAN; 00405 0202 0202 0202 0102 0101: . 0212 - SKP, • 0404 0202 0202 0202 0202 0101 0006 LAN, .0305 0202 0202 0101 01020202. 0213 .SKP,. 0404.0202 0202 0202 0202:0101 0007 LAN, 0404 0202 0202 0202 0202 0101.: 0214 • SKP, .. 0204 0202 0202 0202 02020102 0008. LAN; 0105 0202 02020202 0102 0101_ 0215 SKP, 0104 0202 0202. 0202 0202 0202 0009. LAN; 0304 0202 0202 0202 0102 0101: 0216.. SKP,.. '0404 0202 0202 0202 02020101- - . 0010 LAN; 0405'0202 0202 0202 0202.0202... 0217 SKP, 0404 0202 0202 0202 0202 0101: 0011' LAN, .0404 0202:0202 0202 0102,0101'i '• . 0218 SKP, 0404 0202 0202_0202 0102 0101 . , 0012 LAN, 0404 0202 0202 0202 0202 Q101. '0219 SKP, -0404 0202.0202 0202 0202.0101 • .0013 ..LAN,.. 0404 0202 0202.0202 .0202'0102 • 0014 .LAN,: 0304 0202 02020202 0102 0102 0001-_RRA, '0304 0202 0202 0202 .0202.0101 0015 LAN, • 0101 0202 0202 0202 , 0102 0102 0002 RRA,. •0404 0202 0202 .02020102 0101 0016' LAN, 0304 0202.0202 0202.0101 0102 '0003. RRA; .0304 .0202 0202 0202 0102 0101 • 0017 LAN, .0404 0202 0202 0202 0202 0102*. .0004. RRA„ • 0303 .0202 0202 0202 0102'0101' .0018 LAN, ..0305 - 0202 0202 010.1 . 0202 0102 0005 RRA; 0404 .0202.020/ 0202 0202 0101 0019--LAN,_0305 0202. 0202 0101 0202 0102:: 0006 ..RRA, • 0404 0202 .0202'0202 0202 0101-- 0020 LAN; . '0204 0202 0202 0202 0202 0101. 0007 - RRA, 0304. 0202 0202 - 0101 0202.0101..: -- 0021: LAN, .0404 0202 0202 02020102 .0101,. *0008. RRA, : . 0304.0202.0202 0202 0202 0101 . 0022 LAN, 0104.0202 0202 0202' 0202 0202 • 0009.. RRA,..:0404. 0202 0202 0202 0202 0101 .0023 LAN; 0305 0202• 0202 0202 0202 0101 : 0010,RRA,' . '0404. 0202.0202 '0202 .0202 0101 ..00241,AN, , :0204 0202 0102 . 0202 0102 0102: 0011 RRA;.*:0304 0202- 0202.0202 0202 0101 ... 0025..LAN;0505 0202 02020101 0202 0102. 0012: RRA-,--.-0304 0202 0202 0202 0202 0101 -9926 . LAN,:• : 0303.0202 0202 - 0101 0202 0101 `0013 RRA, 0404 0202'0202 0202 0202 0101..
.10027 LAN;':' 0305 0202 0202.0101.0202 0101 .0014 .. RRA, '0304 0202'0202 0202.0202 0101 .* 0028 LAN; . 0104. 0202 0202,0202'0102 0101. .. 0015:,RRA,-...0303 0202 0202 0202 0202 0101. 0029 LAN; 0000 0000 0202 0202- 0202 0101 0016 RRA;.; .0404 0202 0202.0202:0202 0101. 0030 LAN; 0105 0202 - 0202 0202 0102 0102..• 0017 ,RRA;: )3304 0202.0202 0202 .0202.0102 0031 LAN; • 0304,0202 0202 0202 . 0202 0202 • . 030300202 02020202 0202 0032 LAN, 0105 020/0202 0202 0202 .0101 .. RRA,. - .0304 02020202 02020202 0101 :. 0033. LAN, 0404 0202 0202.0202 -0202-0202.... 0020: RRA, 0304 02020202 0202 0202.0101 ., . 0034 LAN, 0304 02020202 0101 0182 0202 0021 . RRA, .• 0404 0202 0202 .0202. 0202 0101 0035 LAN, 0105 0202 02020101 0202.0101.. • 0022 RRA,: 0304 0202 0202 0202..0202 0101 • 0036 LAN; 0404 0202 0202 0101 0262 0202 0023 •RRA;:. 0304 0202 0202 0202 0202'0101 0037 LAN, 0304 0202.0202.0202 0102 0102 . 0024 RRA, ; 0304 0202.0202 0202 0202 0101 • 0038 LAN,' 0304 0202 0000 0000.0102.0000'. 0025. RRA, •-.0304 0202 0202 0202.0202 0101 . .0039 LAN, 0304 0202. 0202.0101 0102 0102 0026 .:RRA; 0404 0202 0202 0202 0202 0101 0040 LAN; 0104 0202 02020202 0202 0102 0027 .RRA,.. 0303 0202 0202 0202 0202 0101 . - '004.1 LAN, 0404 0202 -0202 0202 0202 0202 0028 • RRA; 0303 0202 :0202 0202 0202 0101- 0042 LAN, 0104 0202 0202.0202 0202 0102: 0029 RRA, .. 0404 0202 0202 0202-0202 0101 -- .0043 LAN, 0104 02020202 00202'0101 0101 -- 0030 RRA,- . 0304. 0202 0202 0202 0202 0101 0044 LAN,• 0103 0202 0202 0202 0102.0000 0031 RRA,- -.0304 0202 0202 .0202 0202 0101 0045 LAM- . 0105.0202 0202 0202 0202 0101 . ..0032RRA, .0304 0202. 0202 0202 0202 010,I . . 0046 LAN, 0405 0202 02020202 0202 0101... .0033: RRA.; 0304 0202 0202 0202 0202 0101 . 0047 LAN, 0404 0202-0202 0202.0202 0102 0034 RRA,'. '0404 0202 0202.0202 0202 0101 0048 LAN, 0104.0202 0202 0202 0102.0202 0035 RRA, .0304.0202 0202 0202 0202 0101. 0049 LAN;- 0105 0202,0202 0202 .0102.0102: 0036 'RRA, •::_0304 0202 0202 0202.0202 0101 0050 LAN, 0405 0202 0102.0202 0101. 0101 . 0037 RRA, - • 0304 0202 0202 0202 0202 0202 0051 LAN, 0405 0202 0202 0202 0202 0101 0038 - RRA, 0304 0202 0202 0202 0202 0101 0052 LAN, 0405:0202 0202 0202 0102'0101 0039 RRA, 0304 0202 0202 0202 0202 0101 0053 LAN, 0405 0202 0202 0202 0102 0102 0040.RRA; 0404 0202 0202 0202 Q202 0101 :. 0054 LAN, 0405 0202 0202 0101 0102 0101. 0041 RRA, 0303 0202 0202 0101 0202 0101 0055 LAN; 0103.0202.0202 0202 0102 0000 0042 RRA, • 0404 0202 0202 0202 0202 0101 0056 LAN, 0505 0202 0202 0202 0202 0101 0043 RRA, • .0303 0202'0202.0202 -0202 0101 0057 - LAN; 0404 0202 0202 -0202.0202 0101 0044 RRA; .0404 0202 0202 0202 0202 0101 0058 • LAN, 0104 0202 0202 0202 0202 0102 0045 RRA, 0304 0202 0202 0202 0202 0101 0059 LAN, - 0105 0202 0202 0202 0202 0102 0046 RRA, • 0304 0202 0202 0202 -0202 0101 0060 'LAN,. 0194. 0202 0102.0202 0202 0101 0047 RRA, 0304 0202 0202 0202 0202 0101 0061 LAN, 0304 0202 0202 0202 0202 0102 0048 RRA, -0304 0202 0202 0202 0202 0101 0062 LAN, :• 0104 0202 0202 0202 02029102 0949 RRA,- 0304 0202 0202 0202 02020101 -
. .0063 LAN, 0305 0202 0202 0202 0202 0101 0050 . RRA, .0304 0202 0202 0202 0202 0101. 0064 . LAN, 0104 0202 0202 0202 0202 0101 0051. RRA, 0204 0202 0202 0101-0202 0101 0065. LAN, 0203 0202 0202 0202 0202 0101 . 0052 RRA; 0204.0202 0000 0000 0000.0000, 0066 : LAN, 0405 0202 0202 0202 0202 0101 0053 RRA, 0204 0202 0202 0101 0102 0102 .• 0067 . LAN,' 0104 .0202 0202 0202 0102 0101 0054 RRA, 0404 0202 0202 0202 0202 0102 0068._ LAN; .0505 0202 02020202 0202 0101".. '0055 RRA, 0404 0202 0202 02020202 0101 0069. LAN, 0404 0202 0202 9202 0202 0102. • 0056 RRA, - 9404 0202 0202.0202 0202 0101 0070. LAN,. 0404 0202- 0202 0202 0102'0101 .0057 RRA,. 0404 0202 0202 0202,0202 0202'. 0071 LAN; : 0104 0202 0202 0202 0202 0101 0058 RRA, I 0204 0202. 0202 0101 0202 0101 . . - 0072. LAN,: 0105 0202 0202 0202 0102 0202 • • 9059 RRA,. 0204 0202 0202 0101 0102.0101 '0073 LAN, • 0405 .0202 0202 0202 0202 0101 0060 •RRA, 0204 0202 0202 0101 02020101 0074. LAN, .0103 0202 02029202 0102.0101 . 0061 RRA, . 0404 0202 0202 .0202 0202 0101 . : 0075 .LAN, •• .01040202.0202.0202:0102.0102. . 0062 RRA, • -.0202 0292 0202 0202 0102 0101 .0076. LAN, -..0105 0202 0202 0101'0101 0102 . 0063 ..RRA, 0204 0202 0202 0202 0202 0101 0077 LAN; 0505. 0202 0202 0202.0202 0102 • 0064 RRA, . 0202 0202 0202 0202 0102.0101
: : 0078, LAN, . 0404 0202 0202 0202 0102 0101 . 0065 RRA, • 0204 :0202 0202. 0202 0102 0101 •• 0079• LAN,. 0505_02020202.0202 0102 0101 0066 • RRA, 0404.02020202 0202 0202 0101. 0080. LAN, .. 0305 0202 0202 0202 0202 0101 0067 RRA,-. 0204 0202 0202 0202.0202010r- 0081 .LAN, - 03040202 02020202.0102 0101 006.8 RRA, 0404 0202 0202 0202 0202 010.1. :• 0082. LAN, 0.104 0202 0202 0101 ,02020102 0069 RRA; .0404 0202 0202 0202 0202 0101
. .0083 LAN, -0505: 0202 0202 0202 0102.0102 0070 .RRA, 0404 0202 0202 0202'0102 0101 • 0084 LAN,. .0405 0202 0202 0202 0202 0102 0071 RRA, '04049202 0202 0202 0292 0101 0085 LAN; 0404.0202 0202 02020202 0101 . - 0072 RRA, 0404 0202 0202 0202 -0202 0101 - 0086 . LAN, :0404 0202 0202 0202 02020102 0073 RRA, _0204.0202 0202 0202 0202 010 1. • 0087 LAN - 0104 0202 0202 0202 0202 0102 .0074 RRA, . 0404. .0202 0202 0202 0202 0101 0088 LAN, • 0305 0202 0202 0202- 0202'0101 . .0075 'RRA; 0204 0202 . 0202 0202.0102' 0101 .0989 LAN; :0304 0202:0202 0202 0202.0101 : • .9076. RRA, 0404 0202 0202 0202 0202.0101 0090 LAN, , .0505 0202.0202.0202 0102 0102. 0077 RRA, 0294 0202 0202 0202 0202 0101.. . 009,1 LAN; . ,i03040202 0202 0202' 0202 0101 . . 0078. RRA, '.0404 02020202. 0202 0202 0101 0092 'LAN, 0405 0202 0202 020.2.•0202. 0101'•• 0079 RRA,.. 0404 02020202. 0202 0202 0101. 0093. LAN; '0203 0202 0202 0202 0202'0202 0080 -RRA, 0404.0202 0202 .0202 0202 0101::' 0094 LAN, 0103 0202 0202.0101 0202 0102 .. 0081 RRA,. 0204 0202 -0202 0202 0102 0101- . 0095 LW 0105 0202.0202. 0202,01010102 0082 -RRA; 0404 0202 0202 0202 0202 0101 0096 LAN; 0405 0202 Q202 0202 0202 0202 . 0083 RRA, . 0404 0202 0202 0202 0202 0101 . 0097 LAN, • 0404 0202 0202 0101. 0102 0102 .. 0084 :RRA,... 0204:02020202 0202' 0202 -0101. 0098 LAN,: .0405 0202 0202.0101 01020102 0085.. -RRA; • 0404.0202 00000000 0000 0000 0099 LAN, '0405 0202 0202 0202.0202 0102 • 0086 RRA, - 0204 9202 0202 0202 0202 0101 0100 LAN, • 0204 0202 0202 0202 0202 0101* 0087 ARA; 0204 0202.0202'0202 0102 0101, 0088 RRA; 0204 02020202 0202 0102 0101 0001: MER,-. 0404.0202 0202-0202 0202 -0101: .0089 _RRA, 0404 0202 0202 :0202 0102 0101 0002 MER; 0304 0202.0202 0202 0202'0101 0090 RRA, , 0404 0202 0202 0202 0202 0101 -- • .0003 : MER,. 0404.0202 0202 0202 01019101 . 0091 RRA, -0404. 0202.0202 0202 0202 0102 0004 MER, 0303 .0202 0202. 0202.02020102 . 0092 RRA, 0404 0202 0202 0202 . 0202 0101 . 0005 MER; 0304 0202 0202 020291020101 .• 0093 RRA, 0404 0202 0292 0202 .0202 0101.- 0006. MER, 0304202.0 0202,0202 0102:0101 0094 RRA, 0204 0202 0202 0202 0202 0101 - .0007 MER; : '0304 0202 0202,0202 -0102 0102 0095 RRA, -0404 0202 0202 0202 0102 0101 . 0008 MER, 0404 0202 0000.0000 0000 0000.. 0096 :RRA, 0204 0202 0202,0202 0202 0101 . 0009 MER,. 0404 0202 0202 0202 0102 0102 '• .0097 RRA, 0204 0202 02020202'0102 0101 0010 MER; 0303 0202 0202' 0202 0202 0101 • 0098 -RRA, 0404 0202 0202 0202 0102 0101 . 0011 MER, 0304 0202 0202 0202 0102 0101 .- 0099 RRA, 0204 0202 0202 0202 0102 0101 0012 MER, 0303 0202 0202 0202 02027,0101. 0100 RRA, 0204 0202 0202 0202 0102 0101 0013 MER, 0304 0202 0202 0202-0202 0101 0101 RRA, 0505 0202 0202 0202 0101 0101 0014 . MER; 0404 0202 0202 0202 0102 0202 0102 RRA, 0404 0202 0202 0202 0202 0101 0015 -MER, 0303..0202.0202 0202 0102 0202 0103 RRA, 0405 0202 0202 0202 0102 0101 0016:•MER, 0404 0202.0000.0000 0000 0000. 0104 RRA, 0405 0202 0202 0202 0101 0102 0017 MER, 0304 0202 0202 0202 0101 0101 0105 RRA, 0204 0202 0202 0202 0202 0101 0018 MER, 0304 0202 0202 0202 0101 0101 0106 RRA, 0405 0202 0202 0202 0102 0102 0019 -MM,. 0304 0202 0202 0101 0102 0101 0107 RRA, 0404 0202 0202 0202 0101 0202 0020 MER, 0303 0202 0202 0202 0102 0102 0108 RRA, 0405 0202 0202 0202 0102 0102 0021 MER, 0404 0202 0202 0202 0101 0102 0109 RRA, 0204 0202 0202 0202 0202 0101 0022. MER, 0304 0202 0202 0202 0102 0102 0110 RRA, 0204 0202 0202 0202 0102 0102 0023 MER, 0404 0202 0202 0202 0102 0102 0111 RRA, 0405 0202 0202 0202 0102 0102 0024 MER, 0303 0202 0202 0202 0202 0101 . . 0112 RRA, 0404 0202 0202 0202 0101 0101 .0025. MER, 0304 0202 0202 0202 0202 0202 0113 RRA, 0405 0202 0202 0202 0202 0102 0026 MER, 0304 0202 0202 0202 0102 0101 0114 RRA, 0404 0202 6202 0101 0202 0101 `0027 •MER,.. 0303 0202 02020202 0101 0101 0115 RRA, 0404 0202 0202 0202 0202 0202 0028 . MER,. 0304.0202.0202 0202 .0202 0101. 0116 RRA, 0404 0202 0202 0202 0202 0102 0029 MER, 0304 0202'0202 0202 01010202 0117 RRA, 0404 0202 0202 0202 0102 0101 0030. MER,- 0304 0202 0202 0202 0.101 0101 0118 RRA, 0204 0202 0202 0202 0102 0102 . 003. 1 MER, 030+0202 .0202 0202 0102 0102, 0119 RRA, 0204 0202 0202 0202 0202 0101 0032 MER, 0303. 02020202-0101 0101 0202 0120 RRA, 0404 0202 0000 0000 0102 0202 0033 .MER; 0404.0202. 0202 0101 0101.0101: • 0121 RRA, 0405 0202 0202 0202 0102 0102 0034 MER, 0404- 0202 0202 0101 02020202.: 0122 RRA, 0404 0202 0202 0202 0102 0101 0035 MER, - 0303 0202 0202 0101.0102 0101 • 0123 RRA, 0505 0202 0202 0202 0202 0101 0036'. MER, 0303 0202 0202 .0101 0102.0202. 0124 RRA, 0405 0202 0202 0202 0101 0101 .0037. MER; 0304 0202 02020202 010.1.0102 0125 RRA, 0404 0202 0202 0202 0101 0101 0038 MER; 0303 0202 0202 0202 0202.0101 - . 0126 RRA, 0405 0202 0202 0202 0101 0101 0039 MER, 0404„0202 0202 0202 0202.0202 • 0127 RRA, 0404 0202 0202 0101 0101 0101 0040 MER, 0304 .0202 0202 0202 01020102 - 0128 RRA, 0404 0202 0202 0202 0102 0101 0041' MER, 0404..0202 0000 0000 0000 0000 0129 RRA, 0304 0202 0202 0202 0102 0101 0042 MER, 0103 0202 0202'0202 0202.0101 0130 RRA, 0304 0202 0202 0202 0102 0102 .0043 MER, 0303 02020202. 0202.0102 0101 0131 RRA, 0404 0202 0202 0202 0102 0101 0044.. MER, 0404 0202.0202 0202 0102'0101. 0132 RRA, 0405 0202 0000 0000 0102 0000 0045. MER, 0404.0202 0202 0202 0102 0202 0133 RRA, 0405 0202 0202 0202 0102 0101 0046 MER, 0404 0202 0202 0202 - 0102. 0101 •-. 0134 RRA, 0404 0202 0202 0202 0102 0101 0047 MER; 0304 0202 0202 02020202.0101 .:. 0135 RRA, 0405 0202 0202 0202 0101 0101 0048 MER, 0304 0202 0202 0202 0202 0101 • 0136 RRA, 0405 0202 0202 0202 0202 0101 0049 MER, .0304 0202.0202 02020102'0101 0137 RRA, 0505 0202 0202 0202 0101 0102 0050 MER;. 030+0202 0202 0202 0202 0101 '- 0138 RRA, 0405 0202 0202 0202 0101 0101 0051. MER, 0303 0202 0000 0000 0000 0000 POP 0052 MER, 0404 0202 0202 0202 0102 0101.' 0001 DAM, 0404 0202 0202 0202 0102 0101 0053. MER, 0104 0202 0202 0202 0102 0101 .: . 0002 DAM, 0203 0202 0202 0202 0102 0101 0054 MER; 0304 0202 0202 0202. 0101 0102 • 0003 DAM, 0304 0202 0000 0000 0000 0000 .0055 MER, 0304.0202 0202 0101 0102 9102 0004 DAM, 0404 0202 0000 0000 0000 0000 0056 MER, 0303 0202..0000 0000 0000 0000 .. 0005 DAM, 0404 0202 0202 0202 0202 0202 0057. MER, - 0303 0202.. woo 0000 0000 0000 . 0006 DAM, 0104 0202 0000 0000 0000 0000 0058. MER, 0304 0202.0202 02020102 0101- 0007 DAM, 0202 0202 0202 0202 0102 0101 0059 MER, 0404 0202 0202 0202.0102 0202 0008 DAM, ::04040202 0202 0202 0101 0101 0060 MER, 010+0202 0202 0202 0202 0101 • 0009 DAM, 0404 0202 0202 0202 0202 0101 0061 MER, 0303_ 0202 0202 0202.0000 0202 0010. DAM, 0404 0202 0202 0101 0102 0202 0062 MER„ 0303 0202 0202 0202 0101 0101 0011 DAM, 0404 0202 0202 0202 0202 0102 0063. MER; .0304 0202 0000 0000 0000 0000 . 0012 DAM, 0104 0202 0202 0202 0202 0102 0064 MER; 0404 0202 0202 0202 0102 0101 0013 DAM, 0404 0202 0202 0202 0202 0101 0065 MER; 0303 0202 0202 010101020202 . 0014 DAM, 0404 0202 0000 0000 0000 0000 0066.. MER„ 0404 '0202 0202 0202 0102 0102 .. 0015 DAM, .0104 0202 0202 0202 0202 0202 .0067 MER, 0404 0202 0202 0202 0102 0102 . 0016 DAM, 0404 0202 0000 0000 0000 0000 0068 MER; 0404 0202 02020101.0102 0101_ 0017 DAM, 0203 0202 0202 0202 0202 0101 0069 MER, 0404 0202 0202 0202 0102 0101 . 0018 DAM, 0204 0202 0202 0202 0202 0101 0070 MER, 0404 02020202.0202.0202 0101 0019 DAM, 0304 0202 0000 0000 0000 0000 0071. MER; 0303 0202 02020202. 0101 0101 0020 DAM, 0404 0202 0202.0202 0202 0101 0072 MER,..0304 0202 0202 0202 0101 0202 '0021 DAM, 0304 0202 0202 0202 0202 0101 - 0073 MER; . 0304 0202 0000 0000 0000 0000 • 0022 DAM.; 0303 0202 0000.0000 0000 00.00. 0074 MER, 0404 0202 0202 0202 0102 0202 . 0023 DAM, 0404 0202 0202 0202 0102 0101 0075 MER, 0304 0202 0000 0000 0000 0000 0024 DAM, 0304 0202 0202 0202 01020102 0076 .MER, r. 0304 0202 0202-02020102.0101 0025:DAM, *0404 0202:0202 0202 02020102, 0077 MER, 0303 0202 0202 0101 0102 0101 0026 DAM, 0404 0202 0202 0202 0202 0101 0078 .MER, '0304 0202 0202 0202 0101 0101 0027 DAM, '0404 0202 0202 0202 0202 0101 0079 MER, 0303 0202 0000 0000.0000 0000 0028 DAM,. 0304 0202 0202 0202 02020000 0080. MER, '0104 0202 0000 0000 0000 0000 • 0029 DAM, 0303 0202 0000 0000 0000 0000 0081 MER, : •0304.0202 0202 0202 0102 0101:: 0030 DAM,. 0404 0202 0202 0202 0202 0102 0082 MER,.. *0404 0202 0202 0202 0102 0102 0031' DAM,. .0202.0202 0202 0202 0202 0101 0083 MER; 0304 0202 0000 0000 0102 0000 0032 DAM, '0103 0202 0202 0202 0102.0102 0084 MER; 0304 0202 0202 0101 0202 0101 0033 'DAM,. 0404 0202 0202 0202 0202 0102 0085 MER,:' 03030202 02020202.0101 0101 0034 DAM, . 0204 0202 0202'0202 0202 0101 0086 MER, 0303020202020202 0101 0202 0035 DAM, 0204 0202 0000 0000 0000 0000 0087 MER, . 0404'0202 0202 0202 0102 0202 0036. DAM, 0404 02020000 0000 00000000 0088. MER, '0304.0202 0000 0000 0101. 0000 • 0037 DAM, 0203 0202 0202 0202 .0202.0102' 0089 :MER, :0304.0202 02020101 0202 0101 0038 DAM, 0404 0202 0202 0202 0202 0 .101 0090 MER, 0303 0202 0202 0202 0102 0101: - 0039 DAM; 0204 0202 00000000 0000 0000. 0091 MER,..: 03030202 0000 0000 0000.0000 . 0040 DAM, .0404-0202 0000 00000000 .0000.
.0092 MER, • *0104 0202 0202 0202 0101.0102 : 0041. DAM, ...0404 0202 0202 0202 0202 0101 . 0093 MER, • 0303 0202 0202 0202 0202 0101 0042 DAM; 0404 0202 0202 0202 0202.0102.. 0094 . 0304 0202 0202. 0202 0102 0101.. 0043. DAM,Y..0304 0202:0000 00000000 0000 0095 ..MER; 0404 0202 0202 02020202,0202 .. 0044 DAM . 0304:0202_0202 0202 02020102 0096 MER,...0304 .0202 0202 0202.0102..0101T 0045 . DAM,.. 0404 0202 02020202 0202 0000 0097. MER, 0304 0202 0000'0000 0000 0000. 0046 .DAM;.. 0304.0202 0202 0202 0202'0101 . 0098- .MER-* 0304 0202 0202 0101 0102 0101 .. 0047 DAM,L0404 0202 0000 0000. 0000 0000 . 0099 . MER; :.• 0304.'0202 0202 0202 0102 0102. 0048 :DAM; 0404 0202 0000 0000 0000..0000 P01), • 0049. DAM; 0204 0202 0000 0000 0000 0000 0001 AFN; ...0505 0202 0202 0202 0101 0102 0050:. DAM, 0204 0202 0202 0202 0202 0000 . 0002 AFN, '.0405•0202 0202 0202,0202 0202• 0051 DAM, .0204. 0202 0000.0000 0000 0000
0003'. AFN, ..0104 0202 0202.0202 0102.0101 . - . 0052 . DAM; 0404.0202 0202 02020202 0101- " 0004 . 0404 0202 0202 0202.0102 0102 • 0053 DAM,. 0404 0202. 0202 0202 0202 0202 0005 AFN, 0404.0202.0102.0202 0102 0102 .. 0054 DAM,* 0404 0202 0202 .020202020101.:
0006: :AFK..."0304 0202. 0202 0202 0202 0102 •-• - .0055 • 0404 0202 0202 0202 0202 0202 0007 AF4,, :0305.0202 0202 0202 0202 0102 0056' 'DAM; .0204 0202 0202 0202 0202 0.102
0008 'AFN; 0304'0202 0202 0202 0202 0102 - .0057 DAM; 0404 0202.0202 0101.0202 0102 0009 AFN, 0105 0202 0202 0202: 0102.0101 0058. DAM,. :0404 0202 0202 0202 . 0202 0102 . 0010. .0203.0202 . 0202 0202 0102 0101 0059 :DAM,. .0405 0202 0202 0202.0202 0202 -. 0011 AFN,.: '0304.0202 0202 0202 0202 0102:: 0060' DAM, ,0304 0202.0202.0202 0202 0102 0012.. AFN, 0101 0202 0202- 0101•0102..0101 . 0061 DAM,. 0304 0202 0202- 0202 0202 0101 0013 AFN,' 0404 0202 0202 0202 0102 0102 . 0062 . DAM,.*. 0404 0202 0202.0202 0202 .0102 0014 .AFN,. 0103 0202 0202 0202 0102 0101'.. :. 0063 • DAM; 0404 0202 0000•0000 0000 0000 0015 . 'AFN, . 0405 0202 0202 0202. 0102:0102'r *•0064 . DAM,.. '0404 0202-0202 .0101'0202 0102 0016 .AFN, .0103 0202.0202 0202.0202 0101' . . 0065 DAM, 0404 0202 0202 0202.0202 0102 - 0017 AFN; 0405 6202 0202 0101'01020101 . ' 0066 DAM, 0404 0202 0202 0202. 02020102 0018 .AFN, 0104 0202 0202-0202 0202 0101 0067 *DAM, . 0404.0202 0202 02020202 0102 0019: AFN, 0704.0202 0202 . 0202 0102 0102' 0068 DAM, 0404 0202.0202 0202 0102 0101 • 0020. AFN, :0104 0202 0202 0202 9102 0202 0069 DAM, . 0000 0000 0202.0101:0202 0101 0021 AFN, • 0404-02020202020.2 0202 0101 -. 0071 DAM; 0304 0202 00000000_0000 0000 0022 .AFN; .0404.0202 0202 0202.0102 0102. :0072. DAM; 0304 0202 0202'0202 0202 0101 0023 AFN, • 0303 02020202 0202 0102.0202 0073: DAM; . 0404 0202 0000 0000 0000 0000 0024 . AFN, 0304 0202 0202 0202 0202010.1 . • 0074 DAM, 0304 0202 0202 0101 0202 0101 0025 AFN; 04040202'0102 0202 0202 0101 . .0075* . DAM, • -0104 0202 0202 0101. 0102 0101 0026 .AFN,::.- 0304 0202 0202 0202 0102 0101 ..0076: DAM,. 0304 0202 0202:0101 0202.0102 .0027. 'AFN; : 0304 0202 0202 0202 0102 0101 :• 0077 DAM, 0404 02020202 01010102 0101 AFN, 0404 0202 0202 0202 0102 0102 .0078 .DAM; 0404 0202 0000 00000000 0000 0029 AFN;.. 0303 0202 0102 0202 0202 0102:. 0079 DAM,. .0404 0202 00000000.0000 0000 - 0030 AFN, - 0304 0202 0102 0202 0102 0000' 0080 DAM, • 0404 0202.0000 0202.0101 0101 0031 AFN, 0304 0202 0202 0202 0102 0102 0081 DAM, 0.404 0202 0202 0202 0102 0102 0032. AFN, 0104 0202 0202 02029202:0102. 0082 DAM; - 0404 0202 020/0202 0202 0102 0033 AFN, - 0304 0202 0202 0202 0202 0101 0083 DAM, 0404 0202. 0202 0202 0202 0101 0034 AFN, 0404 0202 :0202 0202 0202 0101 0084 . DAM, 0404:0202 0202 0202 0202 - 0101 0035 AFN, '0304- 0202 0202 0202 0202 0102 0085 DAM, :0404'0202 0202 0202:0202 0000 0036 . AFN,. 0104 0202 0202 0202 0101 0102 0086 DAM, 0404 0202 0202 0202 0202 0101 0037. AFB, 0404 0202 0202 0202 0102 0101 0087 DAM, 0404 0202 0202 0202 0202 0000.. 0038 AFN, 0104 0202 0202 0202 0102 0 .101- 0088 DAM, :0404 0202.0202 0202 0202 0000
0039. AFN, • 0303 0202.0202 0202 0202 0101 0089 DAM, 0404 0202 0202 0101 0202 0102 0040 . AFN, 0103. 0202 0202 0202 0202 0101.. 0090 DAM; 0404 0202 0202 0202 0202 0101 0041 . AFN, 0404 0202 0202 0202 0202 0101 • • 0091 DAM, 0404.0202 0202 0101 0202 0101. 0042 AFN, 0304 0202 0202 0101 0102-0102 :. 0092 DAM, 0404 0202 0202 0101. 0202 0102 0043. AFN, .0404.0202 0202 0101:0202 0101 '.0093 DAM, 0404 0202 0202 0101.0202 0101 0044 AFN, • 0303 0202 0202 0101 0202 0102 • 0094 . DAM, 0304 0202 0202 0101 0202 0101 0045 AFN, 0104 0202 0202 0202 0102 0101. 0095 .DAM, -.0404 0202 0202 0202 0102 0101 0046 AFN,. 0304 .0202 0202 0202 0202 0102 0096 .DAM, . 0405 0202'0202 0202 0202'0102 .. 0047 AFN, 0405 0202 0202.0202.0102 0101 .. . 0097 DAM, 0404 0202 0202 0202 0202 0101 ... 0048 .AFN, .0405 62020202 02020102 0101 0098 :DAM, • .0104 0202 0000 0000 0000'0000: 0049 AFN, . 0404 0202'0202 0202 0202 0101: 0099.. DAM, 0404 02020202 -0202 0102 0101 0050. AFN, 0404 0202 0202 0202 02020101 0100 DAM, '0404 0202 0202 0202 0202 0102. .0051. "AFN, -.0103 0202 00000000.0000 0000 • POP ' • 0052 0305 0202 0202 0202 0101 0102 . 0001 SPE,.: .0104 0202- 0202 0202 0202.0000 .0053 AFN, 0304,0202.0202 0202 02020202 ... 0002 - SPE, 0104 0202 0202 0202 0202 0101 0054 .AF14,. '0204. 020/0202 0202 0102,0101 0003 SPE, 0104 0202. 0000 0000 0000 0000 0055 AFN,, 0204.0202 0202 0202 0202:0102 •• 0004 SPE,: 0104 0202 0000 0000.000.0 0000... 0056 AFN,. ;0303 0202 0202 0202 0102 0101 .. 0005 -•SPE, . 0104:0202.0202 0202 02020101 .. 005T': AFN, 0103 0202 0202-0202 0202 0101 0006 . SPE;, :0404 0202'0202 0101 0202 0102 0058 ..AFN„..0103. 0202. 0202 0202 0102 0101 0007 SPE,. 0104 0202 .0000 . 0000 0000:0000'• :.005.9'..AFN,....:01040202 . 0202 0202 0202 0000 0008. SPE; 0404 020/0202-0202-02020102 0060 .AFN;• :0303.0202 0202 0202 0102 0101 0009 SPE, : 0104 0202 0000-0000-0000 0000 0061 AFN, 0304.0202 0102 0202 0202 0101. 0010. SPE, , 0404 02020202 0101. 0202 0101. 0062.. AFN,..,0304 0202 0102 0202 0202 0202 .0011 . SPE,: -0104 0202 0000.0000 0000 0000 .0063. AFN, -0404 02020000.0000 0000:0000: 0012: SPE, 0104 0202 0000 .0000.0202 0000. 0064 AFN, .0203.0202 0202 0202 . 0202.0102 0013...SPE, ...0104 0202 0000. 0000 00000000 0065 AFN, ., 0104 0202 .0202 . 0202.0102 0202 .0014. SPE, 0104 0202.0202.0202 0202 0101' • 0066 AFN,• • 0303 0202 0202 0202 0102 0101 0015 SPE, .0104 0202 0202 0202 0202 0102 0067 AFN;''..0404. 0202 0202 0202 0202 0101 . 0016: SPE, 0104 0202'00000000. 0000 0000 0068 ;AFN,.. 0404 '0202 0202.0202 0202.0101 ,••• 0017. SPE, • 0104 0202-0202 0202 0202 0101, 0069 AFN,:: 0404:0202 0202 0202 :0101 0101. 0018 SPE, 0101 0202 .0202 0202:0202 0102 : 0070 'AFN,..,0305 0202 0202 0202 0101 0102.-:' 0019. .SPE,, 0304 0202:0000 0000 woo-bow • 0071. AFN,. 0303 0202 02020202 0202 0102... 0020.. SPE, .0104 0202 0000 0000 0000 0000 0072 ..AFN, .0103 0202 0202 0202 0101 0101 0021 SPE, . 0101 0202 0202 0202 0202 0101 0073. AFN, .0304.0202, 0202. 0202 -0102 0202. ....0022.1.SPE, .0101 0202 0202 0101 . 0202,0101 . --
0074 AFN , .0404 0202 0202.0202 0202 0101: 0023, SPE.,' '0104 0202 0202 0202 0202 0101 . 0075.. AFN, :• 0304 0202 0202 0202 01020102 0024 ,SPE, 0101 0202 0000 0000 0202 . 0000. .0076 AFN; • 0104 0202 02020202 0202 0102 :. 0025 SPE, . 0104'0202 0000 0000 0000 0000 0077 •AFN; .0104.0202 0202 0202 0202 0101 0026 SPE, 0104 0202 0202.0202'0202.0101 0078 AFN, • •0305: 0202 0202 0202 0101 0102. 0027 SPE,. 0101 0202 0000 0000 0202 0000 0079: AFN, :0305 0202 0202 0202 -0202.0202 . 0028 -.SPE,- :.0104 0202 0000 0000 0000- 0000 . 0080 AFN, ...0304 0202 0202 0202 0202 0202 .• 0029 SPE, - 0104 0202 0202 0202 0202 0101 . 0081 AFN;' . 0304 02020102 0202_0102 0102 :.0030 SPE, 0104 0202 Q000 0000 0202 0000 0082 AFN, •. 0404 0202 0202 0101 0202: 0000 0031 SPE,- • 0101 0202 0202. 0202 0202.0101 0083. AFN, 0304.0202 0202 0202 0102'0102 0032 SPE,. 0104 0202 0202 0202 0202 0.102 0084 'AM,. .04040202,0202 0202 0202 0101 0033 SPE; 0204 0202-02020202 0202 0101. .0085: 'AFNi 0104 02020202 .0202 0102 0202 0034. SPE, .0204 0202 0000 0000 0202 0000 0086. • AFN, 0704 02020202 0202 0102 0101. . 0035 SPE; 0704 0202 0202 0101 0202 0202.. 0087 :AFN, :0704 0202 0202 owl 0202.0101 *0036 SPE, 0104 0202 0202 0202 0202 0101 .. 0088. AFN, .0104 0202 0202 0202'0102 0101 - . 0037 SPE, 0404 0202 0000 0000.0000 0000 0089 AFN,- .0304. 0202. 0202 0202 0202 0101 : 0038, SPE, 0404 0202 0000 0000 0202 0000 0090 AFN, 07040202 0202 020/0202 0101:- 0039 SPE, 0104 0202 0202 0101 0202 0102 0091 AFN, 0404 0202 0202 0202 0202 0102 0040 SPE, 0404 0202 0000 0000 00000000' . 0092 AFN,. 0404.0202 0102 0202.0202 0101 .* 0041 SPE, 0404-0202 0000 0000 0202 0000 • 0093 Ap-74, 0204 0202 0202.0202 0102 0101 •- 0042 SPE, 0102 0202 0202 0101 0202 0202 0094 AFN; 040410202 0202 0101.0102 0101 0043 SPE, 0204 0202 0202 0101.0202.0202 *0095 AFN, 0101 0202 0202 0202.0102 0101 0044 SPE, 0102 0202 0202 0101:0202 0102 0096 'AFN, 0404 0202:0000 pow 0000'0000 0045- SPE, .0102 0202 02020101 0202 0101 0097 AFN; 0305.0202 0000.0000 0102 .0000 . 0046. SPE, 0204 0202 0202.0.1010202 0101. 0098 AFN, 0304 0202 0000.0000 0000 0000- 0047 SPE,.. 0202 0202.0202 0101 0202,0202 0099 'AFN, 0304.0202 0202 0202 0202 0102 0048 SPE, 0204 0202 0202 0101 0202 0101 0100 AFN, 0304 02020202.0202 0102 0101 • 0049 SPE, 0202 0202 0202 0202 0202 0202 POP 0050 SPE, .02040202 0202 0202 0202.0102 `0001 NAM, 0304.0202 0202 0202 0202,010.1 • 0051' SPE, 0202 0202 0202 0202 0202 0101 0002 NAM, 0304 0202,0202 0202 0202 0202 *0052 .SPE, 0202 0202 0202-0202 0202 0102 0003 NAM, 0404 0202 - 0202 0202 0202 0202 *, POP • .0004.NAM;: • 0404 0202 0202 0202 0202 0202 '0001 .DOM, 0304 0202 0202 020202029101. 0005 NAM; 0404 0202 0202 0202.0202 0202 0002 DOM; 0102 0202 0202 0202-02029101' 0006 NAM,- 0404 0202 0202.0202 0202 0202 0003 DOM,. 0204'0202 0202 0202 0202 -0202 0007 NAM; 0104 0202 0202 0202 0202 0202. 0004 DOM, 0405 0202'0202.0202 0102 0101 0008 NAM, 0404 0202 0202 0202 0202 0102-• : 0005 DOM;. 0404 0202 0202 0202 0202 0101 - .0009 NAM, 0104 0202 0202 0202.0202 0101.. • 0006 DOM, 0204 0202 0202 . 0202 0102 0102 . ..0010 NAM; . 0394 0202. 0202 0202.0202 0101 • .0007 DOM,' -0404 0202 0202 0202 0202 0101. 0011 NAM, 0104 0202 0202 0202 0202 0102 .. .0008 .DOM, - • 0204 0202 0202. 0101 0202 0101: 0012 NAM,' 0404 0202 0202 0202 0202 0202 • 0009':DOM,.' 0202 0202.0202 0101 0202 0102 0013 NAM; 0404 0202 0000 0000.'0000 0000.- 0010 DOM,. 0203 0202 0202 0202 0202 0101. : 0014 NAM, - 0104 0202 0202 0202 0202.0102' 0011 .DOM, 0204 0202 0202 02020202 0000: 0015 NAM;:. 0104 0202•0202.0202 0202 0101. 0012 DOM, '0204 0202.0202 0202.0102 0000 0016 NAM,. 0404 0202:0202 0202 0202 0101.. 0013 DOM; 0104 0202 0202 0101 0202.0101'
0017 NAM; 0104 0202 0202 0202 0202 0102 . 0014 .DOM„, .0204 0202 0202 0202 0102 . 0102 0018 NAM, .0304 0202 .0202 0202'0202 0101 0015 • D.OM,• 0405.0202 0202 0202 0202.0102 0019 NAM, .0404 0202 0202 0202 0202 0101 . 0016 .DOK . 0404 . 0202 0202 0202 .0202 0102 0020 NAM, 0404.0202 0202 .0202 0202 0101; 0017' DOM, . 0203. 0202 0202 0202 0102:0102-.. 0021 NAM, :0104:0202 0202 0202 0202 0102 0018 DOM; 0102 0202 0202 0202 0202 0102' 0022 NAM, 0104 0202,020.2 0202 0202 0101 0019 •DOM,• '0404.0202 0202 0101 0202 0102. 0023- NAM, 0404 0202 0202 0202 0202 0102 . 0020 .DOM,.. -04040202 0202 0202.01020101 0024 NAM, -01040202 02020202 0202 0101 0021. DOM, -.0104 0202 0202 0101 0202 0101 0025 'NAM, 0404 0202 0202 0202 0202 0101 0022 . DOM, 0202 0202 0202 0202.0202.0000- 0026 NAM; - 0104 0202, 0202 0202 0202 0101:: . :0023 DOM, • 9204 0202 0202 0101'0202'0101.. 0027, NAM; : 0404 0202'0102 - 0202 0202 0101'. 0024 -DOM;...04040202 0202 0202 0202 0102 • .0028 NAM, 0104 0202 0202 0202 0202 0101 0025 DOM,: 0202. 0202.0202.0202 0102 0101 - 0029 NAM; :.0404 0202 0202 0202 0202 0102: 0026'DOM,. 0202 0202 0202 0202 0202 0102 0030 NAM, ::0104 0202 0202-0202-0202 0101 9027* DOM,: 0405 0202 0202-0202 0102 0101 . 0031 'NAM; 0404-0202 0202 0202 0202 0101 . 0028. DOM, , 0404.0202 0202 0202 0202 0101 .0032 NAM, '.9104. 0202 0202 0202 0202 0101 0029 DOM,: 0104 0207 0202 0202 0202 - 0101. 0033 NAM, 01040202 02020202 0102 0101: 0030 DOM; 0404 0202 0202 0202 0102 0101. 0034 NAM, 0104,0202 0202 0202 0102 0101 0031 DOM, 0204 .0202 0000 0000 000.0 0035 NAM,..9404 0202 0202.0202 0202 0101 DOM, 0404 0202 0202 0202 0202 0101 0036 NAM,:: 0101-0202 0202. 0202 0102 0101. 0033. DOM; .0304.0202 0202 0101 0202 0102. 0037 NAM, , 0104 0202 0202 .0202'0202 0101 ..0034 : 0202 0202 0202 0101 0202 0101.. 0038 NAM, 0101 0202 0202 0202,0202 0102. 0035 DOM, .0405 0202 0202 0202 0202 0202 0039 NAM,- 0404 0202 0202 0202 0202.0101 0036 DOM, 0204 0202 0202 0202'0102'0202 0040 NAM,• 0103_0202 0202_0702-.0102 0101 0037 DOW- 0204_0202 0202 0202 02020101, 0041. NAM, 0104' 0202 ,0202_0202 0202. 0101 0038 DOM,...0202. 0202 0202 0101 0202 0101 0042 NAM, 0104 0202 0202 0202 0202 0202 - :0039 DOM, • 0204 .0202 0202 0202,0202 0202 • 0043 NAM; .0104.0202 0202 0202 .0202 0101 0040 DOM, • 0204 02029202 0101 0202 0101 0044 .NAM,'. 0404 0202.0202 0202 0202 0202 0041' DOM, . 0102 0202 0202.0202'0202'0101 . 0045 NAM,' 01040202 0202'0202 0202 0101 0042' DOM, • 0102 0202 0202 0101 .0202 0202 0046 NAM; ':.0404 0202 02029202 0202 0102 0043 .DOM, 0404 0202 0202 02020202 0101- . 0047 NAM, 0404 0202 0202 0202 0202 .0102 • 0044 DOM, ...0405 02020202 0202 0202.0202 -0048 NAM,' 0104 0202 0202 0202 0202 0102' .0045' DOM, 0304. 0202 0202'0101.0202 0101. 0049 NAM, - 0404 0202 0202 0202 0202 0102 0046 DOM, 0405 020/0202 0202 0102 0102 0050 .NAM, 0404 0202 0202 0202 0202 0102 . 0047 DOM, 0204 0202 0202 0101 0202 0101 0051'. NAM, 0404 0202 0202 0202 0202 0101 0048 DOM, 0204 0202 0202 0202 0202 0101 0052 -NAM,- 0404 0202 0202- 0202 0202 0102 0049 DOM, 0204 0202 0202 0101 0202 0102 0053.. NAM, :0404.0202 0202 0202 0202 0102 0050 DOM, .0102 0202 0202 0101 0202 0102 0054 NAM, 0404 0202 0202 0202 0202 0000 0051 DOM, -0104 0202 0202 0202 0202 0101 0055 NAM,:. 0404 0202 .0202 02020202 0101 0052 DOM, - 01040202 0202 0101 0202 0101 - 0056 NAM, .0104 02020202 0202 0202 0101 0053 DOM, 0102 0202 0202 0101 0202 0101 0057 NAM, 0404 0202 0202'0202 0202 0202 - 0054 DOM, 0202 0202 0202 0202 0202 0102 0058 -NAM, 0404 0202 0202 0202 0202 0102 0055 DOM, 0404 0202 0202 - 02020202 0102 0059 NAM, 0404.0202 0202 0202 02020.102 • 0056 'DOM, 0204 0202 020a 0202 0202 0102 0060 NAM; 0404 0202 0202 0202 0202 .0102 0057 DOM; 0304 0202 0202 0202 0202 0202 0061 NAM, 0404 0202 0202 0202 0202 0101 0058 - DOM, 0404 0202 0202 0101 0202 0101 0062 NAM; '0404 0202 0202 0202 0202 0101 .- 0059 .DOM, 0202 0202.0202 0202 0102 0102 0063, NAM, 0104 0202 0202 0202 0202 0101- 0060 DOM, 0404 0202.0202 0202 0202 0101 0064:NAM, -0304 0202 0202 0202 0202 0101 0061- DOM, 0202 0202 0202.0202 0202 0101 - 0065 'NAM,.. 0404 0202 0202 0202 0202 0101 0062 DOM, 0404 02020202 0101 0202:0101. 0066 NAM, 0404 0202 0202 .0202 0202 0101 0063.. DOM, 0203 0202 0202 0101 0102 0101 -0067 NAM,: 0404 0202.0202 0202 0202 0101 • 0064 DOM, 0405 0202 0202 0202.0102 0101- 0068 NAM; 0404 0202 0202 0202 0202 0101 0065 DOM, 0204 0202 0202 0101 0202 0101 0069. NAM, 0000 0000 0202.0202 0202 0101 0066 DOM, 0304 -0202 0202 0202 0202 0102.. 0070 NAM, . 0404 0202 0202 0202.0202 0101- 0067 DOM,.. 0204 - 0202 0202 0202 02020101 .0071. NAM, 0104'0202 0202 0202.0102 0101, 0068 -DOM, 0104 0202 0202 0202 0202 0202 0072 .NAM; 0404 02020202 0202 0202 0101.. 0069 DOM, 0304. 0202 0202.0202 0202 0102 .0073. NAM, . 0101 .0202 0202.02020202.0101 '0070- ,DOM,- .0404 0202 0202-0202 0202 0102 . 0074 NAM, 0104 0202 0202.0202 0202.0101 .0071 . DOM, 0405 0202 0202 0101 0202 01.01 0075 , NAM, -0104.0202 .0202 0202 0202 0101 0072 DOM, 0204 0202 0202 0202'0202 0101 .0076-, NAM, 0404 0202 0202 0202 0202 0101 . 0073 '.DOM,.. 0405.0202.0202 02020102 0202.. 0077. 1.NAK .: 0404'0202,0202 0202 0202 0102_ _0074_ DOM;: 0204 0202. 0202 0202 0202.0101 _0078 :NAM, 0404'02020202 0202 0202 -0101' .00.75 - DOM; 0104- 0202.0202 0202 02020101.,+ 0079- .NAM,:- 0404 0202 0202 0202 0202 0101: 0076 DOM, 0204 0202 0202 0101 0202 0101, 0080 NAM, • 0404 0202 0202 0202 0202 0101. 0077 DOM; 0204 0202 0202 0202 0202 0101 0081 NAM, 0404:0202 .0202 0202:0202 0101 0078 DOM, 0202 0202 0202 0202 01020101. ' 0082 NAM, ...0104 0202 0000 0000 0000 0000 0079. DOM; 0204.0202 0202 0101 0202 0102 0081. 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. 0093. NAM; .0404 0202.0202.0202 0202 0102. 0090 DOM, 0204'0202 02020202 0202 .0101 0094 NAM, 0404.0202 0202 0202 0202 0102 -.• 0091 DOM, '0204 0202 0202 0202 0202 0202 0095 NAM, 0404 0202 0202 .0202.0202 0101 0092 DOM, .0404 0202 0202 0202 0202 0101 0096 NAM, ' .. 0404 0202 0202 0202 0202 0102 .. 0093 DOM,. .0202 0202 0202 0202 0102 0101 • 0097. NAM, 0304 0202 0202 0202 0202 0202 : . 0094 DOM; 0104 02020202 0101 0102 0101 • 0404 0202..0202'0202 0202 0101 -0095 DOM,- ..0204 0202'02020101 0202:0101.. 0099 NAM, 0404 0202 0000 0000 0000 0000... - 0096 DOM, 0404-0202 0202 0202 0202 0101 0100_,NAM; :_0404 0202 02020202 0202 0101- - 0097:_ DOM, 0204'0202 0202 0.10_1.0202
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- . 0115 -VRO, 0707 0202 0202 0202 0202 0101 • .0025 - -VDOR, 0204 0202 0202 01010202 0102 0116 VRO, 0104 0202 0202 0202 0202 0101 • 0026. VDOR,„ 0103-02020202 . 0202 0202 0101 .0117 VRO, .0704 0202 0202 0202.0202 . 0101 .: 0027. .VDOR, 0304 0202 0202 0202 0202 0101 0118 .VRO, 0204 0202 0202 0202 0202 0101 0028.. -VDOR,. 0104 0202 0000 0000 0000 0000 . 0119 VRO, 0202 0202 0202 0202 0202 0101. 0029 VDOR, 0104 0202 0202 0101'02020102 0120 VRO, 0404 0202.-0202 0202 0102 0101 . 0030. VDOR, 0304 0202 02020202 0202.0101 0121 VRO, 0404 0202 0202 0202 0202.0101.- .0031 VDOR, 0404 0202 0202 0101 0202 0101 0122 VRO, 0104 0202 -0202 0101 0202 0101 POP.- 0123 VRO, 0104 0202 0202 0202 0202 0202.. 0001 ICAR„ 0303 0202 0202 0202 .0202 0202 0124 V.RO, 0104.0202 0202 0202 0202 0102 0002 KAR, .0103:0202 0202 0202 0202 0102 0125 VRO, 0404 0202 0202 0202 0202 0101 0003 KAR, .0303 0202 0202 0202.0202 0202. . 0126 , VRO, 0104 0202 0202 0202 0202 0101. 0004 KAR, 0203 0202 0202 0202 0202 0101 . 0127 .VRO, 0101 0202 0202 0202.0202,0102 0005 KAR, .0404 0202 0202 0202 0202 0202 - 0128 • VRO, 0104 0202 0202-0202 0202 0101 0006 KAR, 0404 0202 0202 0202 0202 0101 0129 VRO, 01010202 0000 0000 0000 0000 0007 KAR, 0103 0202 0202 0202 0102 0101 0130 VRO, 0204 0202.0202 02020202 0101 0008 KAR, 0.102 0202. 0202 0202 0102 0101 0131 VRO, 0104'0202 0202 0202.0202 0101. 0009 KAR, 0103 0202 0202 0202 0102 owl 0132. . VRO, 0203 0202.0202 0202 0202 0101 . 0010 .KAR, 0404 0202 0202 0101 0202 0101 ..0133. . VRO, 0704 0202 0000.0000.0202-0101 0011 KAR, . 0304 0202 0202.0101 02020102 0134 VRO, 0704,0202 0202 0202 0202 0101 0012. KAR, 0103-0202. 0202 0202 0202 0202: .0135.. VRO,:' 0404 0202 0202 0202 0202 0101 00,13 KAR, 04040202 0202 0202 0202 0102 . . 0136 VRO,: 0404 0202 0202 0202 0102 0101 0014 KAR, 0404:0202.0202 0202 0202 0101
, •0137 NRO,, 0704 0202 0202,0202 0202 0202 . 0015 KAR, 0204 0202.0202 0202 0102 0101. . • 0138. VRO„ • 0404 0202 0202 0202 0202 0101 0016 KAR, 9103 0202 0202-'0202 0202 0102 0139 VRO, 07010202 0202 0202:0202 0101 . • . 0017 KAR; 0204 0202 0202 .0202 02020202 0140. VRO; :0104:0202 0202 0202 0202 0102 0018 KAR, 0303 . 0202 0202 0202.0202 0101, 0141 ,VRO,' 0404 0202 0202 0202 0202 0202 0019 KAR, .0304 0202 0202 0202 0202 0101 0142 ...VRO,' .0404,0202 02020.101 0202 .0102 ... 0020 KAR, .04040202 0202 02020202 0102 0143. VRO; • .. 0704 0202 0202 ,0202 .0202 0102 0021 KAR, 0204.0202,0202 0202 0102 0102 0144 VRO, 04049202 0202 0101 0202 0102 .0022 KAR, 0303 0202 0202 0202 0202 0101 .::. 0145 VRO, 0.104 0202 0202 0202 0102 0101 : - • 0023 KAR, 0304 0202 0202 0202 0202 0101. 0146.- VRO; • 0204 0202 0202 0202.0202.0202 .0024 ICAR, '0303 0202.0202 0202 0202 0102 01477.VR0,7 0704 0202 0202 .0101 0202 0202 • 0025 - KAR, 0304-02020202-0202 0202 0202 . 0148 VRO, 0104 0202 0202 0202 0202. 0102. 'POP. 0149 , VRO, ,t404 0202 02020202 0202 0102'- -9001 PNO, 0203 0202 0202 0202 0101 0101 0150 VRO,... 0404.0202 0202 0202.0102.0102 .0002 PNO, .0304'0202, 0202 . 0202 0102'0101. . 0151.. VRO, . 0102 0202 0202 0202 0202 0202 . ..0003 PNO, .0303.0202 0202 0202:0202 0102 • 0152 VRO, -.0101. 0202. 0202- 0202 0202 0202. 0004. PNO, 0104 0202 0202 0202 0102 0202. ..0153 •VR0,- • 0405 0202 0202 0202 02920102 .0005 PNG, 0104 0202.0202 0202 0202 0202 . 0154 VRO; 0104 . 0202 -0202 0202 0202 0102 0006 PNO, 0103 0202.0202'0202 0102 -.0202 0155 VRO; .0204 02020202 0202 . 0202.0202 10007 , I:No,. 0103 0202 0202 020201020102' r . 0156 ..VRO, :0404 0202 0202 0202. 0202 0101. : 0008 PNO, 9303 .0202 0202 0202 0101 0101: 0157 .VRO, 0404 0202 02020202:0202 0101 0009 PNO; 0101 0202 0202 02020102 0.101 . 0158 ..VRO;, 0104 -0202.0202 0202 0202 0202 0010 PNO, 0101 0202.0202 02020202 0101 0159 . VRO, ':0104 0202 0202 0202 0202.0202 . 0011 Iwo,. -0304 0202. 0202.0202 0202:0202 .. 0160 VRO, 0104 0202 0202.0202.0202 0101. • 0013 PNO; 6304 .0202.0202 0202 0102 0202 , POP . . 0014 PNO„ 0104'0202 0202 0202 0202 0102.: 0001 RRP, 0505.0202 0202 0202 0202 0102 0015 PNO, 0303 0202 0202 0202 0102 0102 0002. RRP, ,02010202 0202 0202 0102. 0101 `0016 PNO, .0101-0202 0202 02020202 0101 • 0003 :ARPE 0304 0202 0202 0101.0102 0202 0017 PNO, 0103 0202 0202 02020102 0102. 0004 . RRP, 0305 0202 0202.0101 0102 0102.. 0018 PNO, 0102 0202.0202 0202.0202 0102 0005, •RRP„- . .0405, 0202 0202 0202.0102.0102 • • • 0020 PNO, 0102,0202 0202 0202 0102 0000 0006 RAP,. 0203.0202. 0202 0101 0202 0202 .: 0021 'PNO, 0102 0202 0202 0101 0202 0202 .. 0007 .RRP,• 0205 0202 .0202 0202 0102 0202 • 0022 PNO, 0103 0202.0202.0202.0202: 0202. 0008 -.RRP, . 0101:0202.0202,0202 0202 0101 .*. POP : 0009 RRP,, 0304 0202.02020202.0202 0202 : 0023 ROM, 0304 0202 0202 02020202 . 0202 0010 RRP; ..0.101 0202 .020202020202.0101 0024 ROM, 0104 0202 0202 0202 02020101
0011` RRP , 0202 .0202 0202 0101.0202 -0101,.: '0025 -ROM, -- 0404 0202 0202 .020202020202 • 0012 RRP, 0104020202020101 0202 0202. - .. ...0026 ROM, 0103 0202 0202 0202 0202 0101 - 0011: RRP, 0102.0202 0202 0202 0202'0102. • 0027 ROM, 0304„020.2 0202 0202 0102 0202. . .0014 -.RRP, 0105 0202 0202 0202.0.102 0102 0028 ROM, 0101.0202.0202.02020202.0202 • 0015. RRP,. • 0205:0202.0202 0101.0202 0102 0029 ROM, '0304 02020202 0202. 9.191 0102 0016. RRP,... 0203 0202 0202.02020 .102 0101 '• :0030 'R0M, 0404 0202 0202 .0202 0102 0102 0017: RRP, -,0405 0202 0202 0101 0102 0101 0031 ROM, 0304 0202 0202 0202 0101 0101. 0018 RRP, 0105 0202 0202 0101 0102 0102 0032 ROM, 0303 0202 0202 0202 0102 0202 0019 RRP, 0105 0202 0202 0202 0102 0102 0033. ROM, 0304 0202 0202 0202 0102 0202 0020 RRP, 0204 0202 0202 0202 0202 0102 0034 ROM, 0103 0202 0202 0202 0102 0102 Title line: "GENEPOP INPUT FILE FOR SIX SHEEP BREEDS" TF ALB CA X HB DIA GLD GPD PGD ES1 ES2 GPI LDH PER pop DOR;- 0204 0202.02020202.0202 0102 0000 0000 0000 0000 0000.0000 0000 0000 . DOR, 0204.0202 0202 0202 0204 0101 0000.0000 0000.0000 0000 0000.0000 0000 DOR, 0404,0204 02020202 0202 0202 0000 0000 0000 0000 0000 000a0000 0000 DOR, .0204. 0202 .0000 0000 .0000 00000000 000a0000 0000 0000 0000 .0000 0000, DOR, . 0404 0202 0202 0202 .02020101 0000 0000 0000. 0000 00000000 0000 0000 : '• 0404 .02020202 0202 0202 0101 0000.0000.0000 0000, 0000 00000000.0000 . DOR, 0204 0202 0202 0202 0202 0101. 0000 - 0000 0000.0000 0000 . 0000 0000.0000 DOR, 0102 0202 0202 0202 0202.0101 0000 000a0000 0000 0000 0000 0000 0000 DOR, 0204. 0202. 0202 0202 -0202 .0102 0000 0000 0000 0000 0000.0000 0000 0000.. • DOR, 0204.0202 . 0000 0000 0000 0000 0000 0000 0000 .0000 0000 0000 0000 0000 DOR, 0404 0202 0000.0202,0000 0000 .0000 0000 .0000.0000 0000 0000 0000 0000 DOR, 03040202.0202 02020202 0101 0000 0000 0000 0000.0000 0000 000T0000 - DOR, 010'10202 0202 0202 .0102.0202'0000 0000•0000 0000 0000.0000 0000 0000 • ...DOR, :0204. 0202.'0000 0000 0000 0000 0000 0000 0000 0000.0000 0000 .0000.0000- DOR,. 0204 02020202 - 0202. 0202. 0102 0000 0000 0000 0000 0000 0000 0000 0000 DOR, 0104 020202029202 02020101-0000:0000 00000000 0000 0000 ..0000•0000. 0404 02020202..0202 0102. 0102 0000 0000 0000.0000 0000 0000 0000 0000.: . • .DOR, 0304 . 0202.-0202 0202 0202 0101.. 0000 0000 0000 0000 0000.0000 0000 0000 DOR,. 0404 0202 0202.0202 0202 0101:0000-0000.0000- 00000000 0000 0000 ..0000 DOR,. 0204 0202 02020101 0202 0101 00090000.0000: 0000 0000 0000 0000 0000 • ..DOR, 0102 0202.0000 .0000. 0000 odoo .0906 0000 0000 0000 0000 0000.0000 0000 .. • 0404 0202 0202 0202 0202 0102 - 0000'0000.0000 0000 0000 0000- 0000.0000 DOR,- 020402020202 0202 020201010000 0000 00000000 0000 .0000.„000 0000 DOR, 0304 0202 0202 0202.0202.0202 0000 0000 0000 0000 0000 0000 0000 0000..• . • . , .. • ..: .• • . • • DOR, 0304 0202,0202 0202- 0202 0201 . 00000000 00000000 900o:0000.0000 0000. •• DOR, 01040202 0202 0101 0202d102:0000 .10000 0000 0000 0000. 0000 0000 pop: • DOR, 0104.0202 0202 62020202.0101 . 0000. 00000000 0000 00000000 0000 0000 DOR; 0104 0202 000.0 0000 .0000 00000000. 00000000. 0000 0000 0000 0000 0000 . DOR, 0104 02020202 0101 0202 .01 .01.0000 0000 0000 0000 0000 000aopoO moo. DOR, 0204 0202 0202 . 0202 0202.01.020000 00000000..00000000 0000 0009 . 0.00.0..• DOR,. 0101 0202 0202 0202 020201.01 0000.0000:9000. 0000 0000 0000 .0000 0000 .• • • DOR,- 04040202 0000 0000 :0000:0000 0000.00000000..0000 . 0000. 0600. 0000 . 0000.. DOR, 01.04.0202'0202. 0202. 0102. 0101:0000 0000 00000000 0000 0000. ,o000 0000. DOR, 0404 0202 0202 0202 0102 0202 00000000. 0000 .0000 0000.'0000..0000 0000 DOR, 0204 0202 0202 0202 •0102. 0101 . 0000 0000.0000 0000 0000 0000 0000 .0000 DOR, 0101 0202'0000.0000 0000 00000000.0000 00000000 .0000 0000 . 0000 0000 • DOR, 02040202 0000 0000 0000 0000 0000 0000-00000000 0000 0000 0000 0000 • DOR, 0204 0202 .0202 0202 0102 0101 0000.0000 .0000:0000 0000 0000 0000 0000 . • DOR,:, 0101 .0202..0000 0000 .0000 00090000. 00000000 0000 0000. 0000 0000 0000 . DOR, 0104 0202 0202 0202 0202 0102 0000 0000 0000 . 0000.0000 0000 0000 0000 DOR, 0204 0202 0202 0101 0202 0101. 0000 0000 0000 0000 .0000 0000 0000 0000 DOR,- 02040202 0202 0101 0202 0101. 0000 0000 0000.0000 0000 0000 0000 0000 DOR, 0404 0202 0202 .0101 .0202-0202 0000 0000 0000 0000 0000 0000 0000 0000 . DOR,- 0204 02020202 0202 0202 0202 0000 0000 0000 0000 0000 0000.0000 0000 - DOR, 0404 0202 02020202 0202 0101 0000 0000 . 0000 0000 0000 0000:0000 0000 . DOR, 0104 0202 0202 0202 0202 0101 0000 0000 0000 0000.0000 0000 0000.0000 ...DOR, 0404 0202.0000 0000..0000.00000000 0000 0000 0000 0000 0000 0000 0000. DOR, 0404 02020202 0202 0202 0101 . 0.000'0000. 0000 0000 0000 0000. 0000 0000. DOR, 0102 0202 0000 0000 0000 0000 0000 0000 0000 0000.0000 0000.0000 0000 DOR 0204:0202 0000 0000 0000•0000.0000 boop•0000 0000 0000 0000: 0000 ;0000 ; DOR, 0204 0202 0202'0202.0202 0101. 0000 .0000 0000 0000 0000•0000 0000.0000
. .:DOR, 0404 0202 0202 0202.0202 0101 00000000o000.:0000.- 0000 0000 0000..0000 DOR, 0204. 0202'0202 0202. 01. 02 0102 0000 6000-0000 0000 0000 000000000000 •••__ DOR, 0102'0202.0000 0000 0000 0000 0000 0000 00000000. • - DOR, 0104 0202 0202.02020202 0101 0000 0000 0000 .0000 00000000 0000 0000 • DOR, 0104 0202 0202. 0202 0202 0101 0000. 0000.0000. 0000 0000 0000 0000 0000 • DOR; 010.4.0202 0202:0202 0102 0101 0000 0000. 0000 . 0000 0000.0000 0000 pop DOR, 0101'-0202-0202.. 0101 0202 01910000 0000 0000 0000 .0000:0000-0000T0000 . DOR, 0204 0202 0202 0202. 0202..0102 0000 0000 0000.0000 i 0000-0000 moo p000.-, DOR; 0204. 0202.0202 0202 0202 0102 0000. 0000 0000 0000 0000 00000000.0000.. DOR, .0404. 0202 0000 0000 0000 000Q 0000 . 0000 0000 0000 0000 0000 0000 poop: DOR; 0204.0202 •0000 0000 0000 0000 0000 0000 0000 . 0000 0000.0000 0000 ...0000i. DOR,.: 0104 0202.0202.0202 0202 0101 0000 0000.-0000.0000..0000i0000.0000 0000 • • `DOR; 04040202 . 0202 0202 0102 0101 . 0000. 0000 0000 0000 0000 0000 0000 0000 DOR . 03040202 0202.0202 0202 : 0202 0000 00000000.0000.0000 ..0000:0000 ..0000 DOR, 0404:0202.0202 0202 0202 .0101 0000. 0000 opoo...000 0000.0000:0000. 0000, DOR; • 0104 0202 0000 0000 0000 0000.0000 0000 0000 0000 0000 0000 0000, 0000•:... DOR, 0204 0202 02020202 0202 0101 0000 0000.0000 0000..00.000000.:0000 .0000 DOR,: 0404,0202. 0202 0101.0202 0102. 0.000:0000. 0000.0000.0000 0000 :0000. moo :•• DOR; 0204 0202. 0000: 0000 0000 0000 .0000 0000 0000 0000 .0000 poop 0000. 0000 . DOR, 0204 0202 .0202:0202 0102.0101:0000 0000 .0000 0000. 0000 0000. 0000 0000. DOR,. 0000 0000:0202 0202 0202 01010000. 0000 0000 0000 0000 0000 0000 0000.
DOR, - 0304. 0202.0000.0000 .00001..0000 0000-0000,0000 woo. oor0000 000b,o0oo DOR, 0202 0202.0202 0202 0202910f9000 .0000 .0000 0000 0000. 0000-0000 rpobo DOR, .01.03 020200000000:0000 0000 0000 0000 0000 0000 0000 00000000.0000 • .DOR, 0404 . 0202,0202 .0202 0202 0101....0000:0000 .0000 0000.0000 0000 0000 0000 . DOR, 0102 0202 . 0202.02020202 0202 0000 0000 0000 0000 0000.0000..000.0.0000. .DOR, 0304 0202 02020202 0202 0101 -0000.0000. 0000 . 0000:0000- 0000 0000, 0000 DOR,: 0304 0202 :0202 0202 0102. 0101 .. 0000, 0000 0000 0000.000p.00Oo 0000 0000 DOR; 0204.0202 0202 0202 0202 0101 0000 0000 0000 0000- 0000 0000 0000 0000 DOR, 0204 0202 0202 0202 0202 0101 0000 0000 0000 . 0000 0000 0000 00000000 DOR,- ..0304:0202 0202 0202 0202 0101 0000 0000 0000 0000 0000 0000 0000 0000 DOR; . 0202 0202 0202 0202 0202 0101 0000 0000 0000 0000 0000 .0000 0000 0000 • DOR, 0202 0202 0202 0202 020210102 0000 0000 0000 00000000 .0000 0000 0000 .DOR, 0000: 0000 0000 0000 0000 0000 01001.01 01.01 0000 0000 0000 0101 0101 DOR, .0000 00000000 0000.0000 0000 0101 0101 0101. 0000 0000 0000 (101. 0101 DOR, 0000 0000 0000 0000 0000 0000 0101 0101 0101;0000 .0101 00000101 0101 . DOR, 0000 0000 0000 0000. 0000 00000101 0101-0000 0000 0000 0000 0101 0101 . : DOR 0000 0000 -0000 0000 Q00000000101 0101 0000 0000 0000 0000 01010102 . DOR, 0000 .0000 0000.0000. 0000 0000.01010101. 0101.0000.-0101 . .0000 0101 0101 • DOR,. 0000 0000 0000 0000 0000 0000 0000. 0000.0000 0000 0000 0101.0101. 0000.:. DOR, 0000 0000 00000000.0000 . 0000 0000 0000-0000 0000, 0000.0101 0101 0000 • DOR,. 0000 0000 0000 0000 :0000 0000.9000. 0000 .0000 0000.0101 0101 .-0101.0000: .DOR, .0000. oopomoo 0000„0009 0000 0000 - 0009. 0000.0000. 0000 0101 0101 0000 DOR, 0000 0000. 0000 0000. .0000.0000 0060000o:0000 00009000 0101 0101 0000 DOR, 0000 0000 0000 0000 0000 0000 000Q 0000i 0000 9000. 01.01 0101 0101.0000 DOR; • 00000000 0000 0000 0000 0000 0000 0000 0000 0000.0000 0000.0101 0000 DOR.- 0000. 0000 00090000709000000 0000 0000 0101. 01010000.10000 0101 0101 DOR,. 0000:0000 00000000 00000000 0000'0000910.1 0000 0000 0000. 0101 0101.• DOR,. 0000 0000 0000 0000 00090000 0000 0000.0000 0000 0000 0000.0101 0101. 0000 0000 . 0090.0000.0000 :0000 000.0-0000. 0000. 01:0.1:.0000. 0000 0101. 0191 - DOR, - .00000000 0000. 0000 0000 -0000 0000_0000.0101 01.01...0000.0000 01010101. • 0000. 0000.0000-0000 0000...0000._.0101: 0101: 0101 0000 0000 0101 0101. DOR; • 0000. 0000 .0000 0000.0000' 0000 0401 .o101 01.01 0000. 0000 0000 0101 0101 DOR;. .:.00000000..0000 0000 0000 000.0 0101 oloi 0101 .0101 . .0000 0000 0101-.0.101 DOR, 0000 0000.0000 -0000.0000..0000..0101 0101.0101010.1 0000 0000 010.1.'01101 DOR, 0000 0.009 . 0000 0000 0000 .0000 0101 0191. 0101 0101- 01010000 0101 :0101 . • DOR; 0000 :0000 0000 0000 00001.0900 0101.0101:010,1 0101 0000 0000 01 ot.01 of DOR, 0000 0000 0000.-0000-moo 0000 .0101 0101 0101.0000. 0000 0000 0101 9101 DOR, 0000: 0000000Q:0000 . 000Q 0000 0101 0101 0101 0101 0000 0000 0101 0101 DOR, 0000. 0000 0000:0000.0000'0000 0101 0.101 :. 0101.0101 0000 0000 0101.0101- DOR, 0000 0000000o .0009 00000009 0191 . 0101 0101 . 0000 00000101 010.1...• .• 'DOR, 0000 0000 p000! 0000 . 0000 0000.0101 0101-0101 0101 0000-0000-0101 DOR,- 0000 0000..0000.00000000 0000 0101. 0101 0101. 0101 .0000:0000 .01.01 0101. pop. VRO, 0204 0202 0202.0202 0202 0192 0000 . 0000 0000%000000 0000 0000 0000 VRO; 0101..0202 0202. 0202 0202 0101 0000.0090 0000 0000 Oooff0000 0000 0000 VRO;....0101 . 0202. 0202..0202..0202.0102 0000.00000000 000Q. 0600 0000. 0000 0000 0104 0202 0202.0202.0202 0102 0000 0000.0000 0000.'0000 0000 0000 0000 VRO, 0404-0202 0202 0202 0202 0101- 0000 .0000 0000 0000,0000 0000 0000.0000 VRO, .0404 0202 0202 0202 0202 0102 0000 0000 0000 0000 0000 0000 0000 0000 VRO, 0404 0202.0202 . 0202 0202 0102. 0000 0000 0000 0000 0000.0000 0000 0000.- • VRO,, 0404.0202 0202 0202 0202 0102 0000 0000 0000 0000 0000.0000 0000. 0000 - VRO,. 0104 0202. 0202:0202 0202 0102 0000 0000 0000 0000.0000 0000 0000 0000 VRO, 0104 0202 0202 0202.0102.0101 0000 0000.0000 0000 0000 0000 0000 0000 . VRO,. 0104. 0202 02020202 0202 9101 . 0000 0000. 0000 0000 0000 0000 0000 0000 VRO, 0104 0202 0202 0202 .0202 0202 0000 0000 9009 0000 0000 0000 0000 0000 .VRO, 0707 02020202 0202: 0202 . 01.02 0000 0000 0000-0000 0000 0000 0009.0000
. .'VRO, 0104 0202 0202 02920202 0102 0000 0900 0000.00000000.0009 0000 . 0900 • VRO, 0304 0202 0202.01.01 .0202 :0102 opoo:0000 0000 0000 0000 0000 0000 poop._ VRO, 02040202 0202 0202 0202 0202 00090000 0000 0000 0000 0000 0000 0000 : ..'VRO, 0101 0202 0202 0101.0202 0202-000900000990 0000 0000 0000 0000 0000 VRO, 0104 0202.0202.0202 0202 0202 0000 0000 0000 0000 0000 0000 0000 0000 VRO; 04040292 02020101,01.01 0202 0000.0000.00090000 0000 0000 0000 0009, vRoi 0104.0202 0202 0202 0202 01020000.0000 0000 0000 00000000 0000 0000 .' VRO, 0101 0202.0202 0101:0102.010t 0000 0000 0900.9909900o 0000. 0000 0000 `VRO. • 010:1, 0101.000.0.0000 0202 01.02 00009000.0000: 0000 0000 .0000 00.00 0000
. •VRO, 0104 0202 0202'0101 0202.0101'0000'00000000 0000 0000 0009.00000000 VRO; 0104 0292.0202. 0202.0102 010.1 0000 0069.00000000..00000000•0000 0000 VRO,, 01019202 0202 .0101 - 02020101 0000 0000 9009 0000 . 00000000. 0000.0000 • VRO, 0707 0202. 0202,0202 0202 0`101.0000.0000 0000.0009 0000..0000 0000 0000 VRO, 0704 . 0202 0202.0101- 0202 0.1 ol:0000 moo .0009 .0099_90o9000p 0000.9000 • VRO,. 010402020202002 0202 01010000 0000- 0000:09009000 0000:0000.0000.,..... 0704'0202.0202 0202 9202..0191 : 0000 0000 9900 - 0000:0009.0000:0000 0000 VRO, 0104 0202. 02020202. 0202 .01.01 0000 .000p- pot* poop: 00090000 0000 0000. VRO, :0101. 0202.020202020202 -0.101-.0000 00000000 0000 0000 000u0000.,:0000 • v!Ro;: .0101 . 0202 0202 0202 0202 01010090 0000 0000 0000 0000 0000 0000 0000 v!Rp, 0101 0202 Q2020202 .0202 0101:,0000. 0000 0000 0000 0000 0000 0000 0000 VRO,; 0101' .02020202 .0202 02020101 0000 0000 0000 9000 . 0000 :0009 woo 0000.-- • VRO, 0104 0202 0202 .02020202 01.01.000000000000 0000 9000 0000 .0000 0000,. VRO,..- -0404 0202 0202 0202 . 0202 0101 0000 0000 0000 0000 0000 0000 0000 0096. 0104. 0202 0202 0202 0202 0101 0000 0000 :0000 0000 0000 0000 0000 .0000 01940202.0202 0101-0202.01 .01.0000 9000 0000 000900090000 0000 . 0000_ VRO; 0101 0202 02020202 0202 0101 . 0000 0000 0000 0000 0000 0000 0000 0000. VRO, 0704 0202 0202 0101 0202:0102 0000 0000:0000 .0000.0000 0000 0000 0000 0104. 0202. 0202 0202 0202 0101 .0000 0000 0000 0000 0000•0000 0000 0000. VRO; 0104-0202. 0202 9101. 0202 0101 0000.00.000000 0009:0000 0000-.0000 0000 VRO, 0101 0202 0202 0101 0102 0101 0000 0000 0000 0000 0000:0000 0000 0000.* , .:VRO; 0101.0202 0202.0202 0202 0101.006 0000 00000000 0000 000000000000 • VRO, . 0404 0202:0202-0202 0202 .01.0.1 . 0000 0000 . 0000 0000 0000 0000 0000:0000 yRo,. 0704.0202 0202 0202 0202 0101 0000 0000 0000 0000. 0000 0000 0000 0000 0104 0202 0000 0000 0000 woo 900.0 0000. 0009.0000 0000 . 0000 .0000 0000 VRO, 0101 0202 0202 02020202 0101 0000 0000 0000. 0000 0000 - 0000 0000 0000 VRO, 0104 0202. 0202 0101 0102 0101 00000000 0000 0000 0000.0000 0000 0000 . • VRO, 04040202 0202 0101 0202.0101 0000 0000 moo 0000 0000 0000:0000 0000 VRO, 0104 0202 .0202 0202 0202 0101.0000. 0000.0000-0000: 0000 0000 .0000 .0000 ••-s• VRO, 0404 0202 0202 0202 0202 0101 0000 0000 0000 0000 0000 00000000 0000 VRO, 0404 0202 0202 0202 0102 0101 0000 -0000: 0000 0000 mo0 0000 0000 0000 • VRO, 0404 0202 0202 0202. 02020101.0000 . 00000000. 0000 0000 . 0000 0000 0000 VRO, 0404. 0202 0202 0202 .02020.101 0000 . 0000 0000 0000.0000 0000 0000 0000 . VRO,, 0404 0202 0202 0202. 0202 0101 0000 0000 .0000 .0000 0000 0000 woo 0000 • • VRO, 010t.02020202. 0202. 0202 0101 0000 . 0000. 0000 0000 0000 0090:0000 0000 yRo, 0104 0202:0202 .0202 0202.0101 0000 0000 . 0000-0000.0000 0000 .0000.0000 'yRo,• 0104 0202 0202 0202 0202.010.1 0000 0000 00000000: 00000000 0000 9000
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DOM, 0102 0202 0202 0202 0102 01010000 0000 0000 0000 0000 0000 0000 . 0000 DOM, 0404 0202. 0202 0202: 0102 0101 0000 0000 0000 - woo 0000 0000.0000 0000 • • DOM,. 0204 .02020202:0202 02020101 0000 0000 0000 00090000. moo 00000000: DOM,.. 0104 .0202. 0202 0202. 0102 0101 0000 0000. 0000 . 0000 0000 0000 0000 0000.. • -DOM,: 0204 0202 0000.-0900.0202:0000 0000 0000'0000.000o -00000000 0000 9000-. .'DOM, 0404 0202 0202:0202 0102.0101 . 0000 0000 0000 0000:0000.0000.00000000 ..'•DOM, 0102 0202 0202 0202.0102 001..0000 0000 0000 0000 0900000.0 0000 0000 DOM, 0000 0000 . 00000000-00000000 0000 0000 0000. 0101 0101 .9000.0101010.1:..:• 0000 0000 0000.0000 0000 0000 9000.000 0000:01010101 0000:0101. 0102 DOM; 0000.0000 0000 0000 0006.0000-0000 0000.0000.0101 0101.0000.0101 0101' DOM,: 0000 0000 0000 0000 0000 0000.0000-0000-0000 0101 0101 0000:0101 0101.. DOM,. 0000 0000 0000 0000 00000000...0000 0000 .0000 0101 0101.0000 0101-.0101'. DOM.; 00000000. 0000 0000 0000 0000.0000 0000. 00000101. 0101 0000. 0101 0101 DOM, 0000 0000 0000. 0000.000000000000 0000 0000 0000 . 0101 . 0101 0101 0000. • 'DOM,.' 0000.-0000.0000.0000 0000 0000 0000 0090.0000 0000 0101 0.101.'0101.0000- DOM, 0000 0000 0900 0000.•90000.000 0000 0000 0600.0906..0101 0101. 0101-900o- DOM ; 0000 0000 0000 0000 0000.0000 . 0000 .0000 0000 0000 0101 0101 0101.0000 .• DOM, 0000 .0000.0000.0000.0000 0000 0000 0000 01.01.0101-:.0101. 0000 0000 0000 90000000 : 0000.0000.0000 0000 0000..0000: 0101.9101 0101. 0000 DOM, 0000..0000.0000 0000 0000 0000 0000 0000 0000'0000 0000 0101.0102 ...; -.• ..• • • ••• DOM, 0000 0000 0000 0000.0000 0000 0000 0000 0000 0000.000 00000401 : 0102 DOM, 0000.0000 .0000 0000, ocipapp0000q woo 0000 0101 0101: oopo 0101.0191 -• DOM, 0009 0000 0000.00000000 0000 0000 0000 0.1010101:6101 .9009 0101 0101 DOM., 0000. 0000 0000 0000 0000 0000 0000 0000. 0101 0101 .0101 0000 0101 0101 ;.: PQM; 0000 00000000 0000 0000 0000 0000 0000.0000 .01 010101. . m oo 0101 .9101... - • . - .. • . . • . • DOM, 0000.0000 0000 0000,0000..0000 0101 0000 0101 T0000 0101_0000 0101. 0000 .•. .Dom, 0000 0000 0000 0000 0000. 0000. 0101 0000.0000 0000 01.01 0000..0101- 010.1 DOM, 0000 0900 0000.0000 :woo 0000.0101 .0101. 0101.0000 0000 0000 0101 0101 DOM, 0000 0000. 0000 0000 0000 .0000 owl .0101 01010000 0101 0000 - 0101 0101 DOM; 0000 . 0000 0000 0000 00000000 0101. 0101 . 01'01. 0000 .0000 0000 0101 .0101 .DOM,: 0000.90000000.0000. 0000 000001.01.0101 0000 00000101 0000 0101,0101 DOM, 0000 0000 0000 0009 0000 0000. .0101: 0101 0000.0000. 0000 0000 0101 0101. 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SAV, 0104.0202 0000 0000 0000..0000 0000 0000 9000 .0000 0000 0000 00000000 SAN", •-. 0303 0202-0000 0000 .00000000. 0000 0000 0000 0000-0000 0000 0000 0000 - SAV, 0104 0202-0000. 0000.0000.0000 0000 0000 0000 0000 0000 0000:0000 0000 SAV,:- 0105 .0202000000000000 0000 0000 0000 .0009..0000 0000 0000 00000000 SAV,..-• 0304 0202. 0000. 00900000 0000. 9000 0000 0.0.090000.0009 00000000 .0000 SAV, 0103 0202 0000 0000 0000 0000 0000 0000 0000 0000 .0000 0000 m090009.: SAV, 0101 0202 0000 0000 0000 0000 0000 0000 00099000. moo 0000 0000 0000 SAV, 0303:0202.0000, 0000 0000 0000 0000 0000. 0000 0000 0000 0000 0000.0000 ' SAV; 0000 0000 00000000 0000. 0000 0000 .0000. 0000.0000 0000- SAV, -0304. 0202:0000 00000000.00000000.00900000 .0000 0000 0000 0000 0000 SAV, 0101 0202 0000 0000 0000 0000 0000 0000 0000 00000000 0000 .0000.0000 0101..0202 0000.0000 0000 0000 0000 0000 0000 0000 00000000_0000 0000 SAV, 0104 0202 0000 00090000 0000.0000 0000 0000 0000-0000-0000 0000. 0000 : • SAV, .9304...0202 00909000 0000 .0000 0000 0000 0000.0000 0000 0000 0000.0000 SAV, 0405 0202 0000 0000 0000 0000 0000.0000 0000 0000 0000 0000 0000 0000. ..: .SAV, 0103.0202 .0000.0900 0000 0000.-0000 0000 00000000 0000 0000 0000 0000 SAV, 0305 0202 0000 0000.0000 0000 0000 00000000 0000 0000 0000 0000 0000. • • SM; 0103 0202 0000...000000000000 0000 0000 0000.0000, 0000 00000090.0090 i.. •••1 SAV,.,..0101. 0202 0000,0000 0000 0000 000900000000 0000 0000:0000 .9000.0000.: 0202 0000 ..0000_0000 .9090 wog 00000600 0000000.0 0000. 00000000. SAV, 0202. 00000000.0000. 0000..0000. 0000 0000 0000 0000 . 0000. .0099 0009 .: sAv,.. 0303 0202'0000 0000 00000000 0000 0000 0000 0000 9000)3000 0000.000G : SAV,:•0303-0202.opop.9000 0000..9000 0000 0000 0000 0000 0000 0000 0000 p000. SAV .0104 0202 0000 0000.pow 0000 0000 0000 0000 0000. 0000 0000 0000 0000:: : SAV,.. -0104 0202 0099.00009000 0000 . 0000 0000 00000000 0000'0000. 0000 0000.. . SAV, 0303 0202 . 0000 0000: 0000-0000 0000.0000 0000 0000 0000 0000 0000.-0000 . SAV.,-- 0101; .0 ..0000 .0000 .0000. 0000. 00000000 0000 '0000 0000 00000000 0000 SAV,:_ 01040202:0000.0000 0000 0000 0000 00000009 0000 0000 0000:0000 0000: . SAV, ..01.04 0202 mo0 0000 0000 moo. 000.9000 0000 0000 0000 . 0000 0000.0000 SAV, 0203 0202 0000 0000 0000 0000 0000 0000 0000 0000.0000 0000 0000 0000 SAV; . 0303 0202 0000 0000 -0000 0000 0000 0000 0000.0009 0000. 0000; 0000 0000 SAV,.:. 0105 0202:02020202 0202 .0202 0000 0000 0000 0000.-0000 0000 0000 0000. • SAV; 0304 0202 0202 0202 0202 0102 0000'0000.0000-0000 0000 0000 0000 0000 :SAW. '9105,0202 0202 0202 0202 0202 0000 0000.0000 0000 0000 0000 0000 0000 • • 'SAV, - 0105 0202 0202-0202 0202 0202. 0000 0000 0000.0000 0000 0000 0000 0000 SAV, 0105 0202 .0202 0202 .0202 0102.0000 0000. 0000_0000 0000 0000 0000 0000 SAV; 0405.02020202 0202 . 0202 0102 0000 0000 0000 0000 0000 0000 .9000 0000 SAV, .0104. 0202.0202 0202 0202 0202 0000 0000 0000..0000 0000 0000 0000 0000 SAV, 0104 0202- 0202 0202 0202 02020000 .0000 0000 0000. 0090. 0000 0000 0000
.SAV, 0105 0202 0202 0202 02020101.0000 0000 0000 0000 0000 0000 0000 0000 SAV, 0105 0202 0202 0202 0202 0202 0000 0000. 0000 0000 0000 000pPoop0000 SAV, 0405 .0202. 0202 0202 0202 0000 0000 0000 0000 0000 0000 0000 0000 0000 .SAV; 0104 0202 0202 0202 0202 0202.0000. 0000 000p 0000. 0000 0000 0000 0000 SAV, 01.01. 0202 0202.0202 0202 0101 0000 . 0000 0000 0000 0000 0000 0000 0000 .SAV, 0105 0202 0202.0202..0102 . 0101 0000. 0000:0000 0000 0000 0000 00000000 0205 0102 0202 0202,.0202 0202 0000 0000 0000 0000 0000'0000* 0000 0000 . SAV.: 0505 0202. 02020202 0202 0102.0.0000000 0000. 0000 0000 0000 0000 0000 SAV, 04050202:0202 0202 0202 0202 oopo. 0000 0000.0000 0000 0000 0000 SAV, 0105 020.2 0202 0101• 0202 0102 0000 .00000000 0000 0000 0000 0000 0000 : :SAV, 0105 0202 0202 9202:02020101 0000. Q000 0000 0000 0000- 0000..0000 0000 SAV,• 0105.02020202.01010202 . 0202 0000 0000 0000 0000 0000.0000 • . • SAV, 0195: 0202-0202.0202.0000. 02020000 0000 0000. 0000. 0000 0000 . 0000 0000 • SAV, 0103. 0202r0202-02020202 0101 0000 0000 0000 0000 0000 0000. 0000_0000 SAV, 0101 0202. 0202 0202...0202.0202.00000000 0000 0000 0000 00000000 0000. SAY, 0105 0202 0202 0202 0202 0101 0000.0000 0000 0000 :0000. 0000:00.00 0000 SAV, 05050202 02020101-0202 0202. 0000 0000 0000 0000 0000 0000 0000 0000 SAV, 0103.0202 0202.0202 0202 01020.000 0000-0000 0000-0000 0000 0000 0000 SAV, 0104.0202.0202 0202 0202 0202 0000. 0000 0000 0000 0000 0000 0000•0000 .SAV, 0405 0202 0202 .0202 0202 0102 0000 0000 000p0000 0000 0000`0000 poop.. .SAV, 01040202 0202 0202 0202,0102 0000 0000.0000 0000 0000 0000 0000. SAV, 0101 0202 0202 0202-0202 0102p000 0000 0000 0000 0000 0000 0000.0000 • 0103 0202 0202 0202 0202 010.1 . 0000 0000 - 0000 0000 0000 000p0000 0000 SAV, 0103 0202: 0202 0202 02020102.0000 . 0000 0000 000000000000.00000000 SAV, 0105 0202 0202: 0202 02029101 . 0000 0000 0000.0000 00000000 0000. 0000 SAY,.: 0101 0202 0202.0202 0202 0202 0000 0000 0000 0000 0000 0000 0000. 0000 SAV, 0105 0202 0202 0202 0102 0102 0000 0000 0000 0000 0000 0000. 0000 0000 .SAV,. 0101 0202-0202.0101 0202 0101 - 0000 00000000 0000.0000 0000 0000 0000 SAV,. -01.01 0202 0202 0202 0202 0102 0000 0000 0000 0000 0000 0000 0000 0000. • SAV, 0104 0202 0202 0202.0202 01.02 .0000 0000 0000 0000 0000 0000 0000 .000- SAV, 0305 0202 0202 0202 0202 01010000 0000 0000 0000 0000 0000.0000 0000.' SAV, 01010202. 0202 .0202.0202 0202 0000 0000 0000 0000 0000 0000 0000 0000 SAV, 0101 0202 020201010202 0102. 0000 0000 0000 0000 0000 0000 0000.0000. SAV, 01040202 0202 .0202'02020202 0000 0000 0000 0000 0000 0000 0000 0000 SAN/; .0105 0202 0202 0101 0202 0202 0000 0000 0000 0000. 0000 0000 0000 0000 SAV, 0101 0202- 0202 .0202-0202 0102 0000 0000 0000 0000.0000 0000 0000 0000 SAV, 0105 0202 0202 0202 0202 0102 0000 0000 0000 0000 0000 0000 0000. 0000 :. SAV, 0105 0202 0202 0202 0102 0102 0000 0000 0000 0000 00009000 00000000 SAV, 0104.0202 0202 0101 0202 0102 .0000 0000 0000 000Q 0000 0000.0000 0000 . SAV, 0101 0202 0202 0202 0202 0102 0000 0000 0000 00000000 0900.0000 0000 •:. • SAV, 0000 0000. 0900 0000 0000.0000 0101 0101 moo .0000 0000 0000.0101. 0101. SAV, 0000 0000. 0000 0000 0000 0000 0101 0101 9000. 0101'0000.0000.0101.0101- SAV, 0000 0000 0000 0000 0000 0000 0101 0101 0000 0000 . 0191.-0000 0101 0101. SAV, 0000 0000 0000 0000 0000 0000 0101 0101 :00000101 0000 0000 0101 0101 SAV, 0000 0000 0000 0000 •0000•9000 0101 0101. 0000 0000 0101, 0000 0101. -0101 SAV, 0000 0000 0000 0000 0000 000091910101 0000 .0000 .0000 0000 0101 0101 SAV, 0000.0000 0000 0000 0000 0000 0000. 6000 0101.-0000 00000101 0101 .0000 SAV, 0000•9000.0000 0000 0000 0000 .00000000.0101,0000 0000 0101 .0101. 0000 .. • SAV, 000.0,-0000 0000 0000 0000 0000 0000 0000..000090060101i0101 0101 0000 .SAV, 0000 0000 0000 0000 0000 0000 0000 0000 0101. 0000 0000 0101 0101 .0000 . •
SAV, . 0000.0000'0000 0000..0000 00000000 0000010100000101 0101 9101 0000.. SAV, 0000.-0000.0000.0000 0000 0000 0000 0000 0101 0000 0000 0101 0101 0000 SAV, 0009.0000 0000.0000 0000. 0000 0000.0000. 0191 0101 0000 00000191 010.1:. SAV, 0000 0000. 0000•0000 .00009000:0009 0000 000010000,0000 0000.0101 0101.., SAV, 0000 0000 0000. 0000 0000 0000 0000 0000 0101 0101 0000 0000:0191 =0102 SAV, 00000000 0000 . 0000 0000.0000 0000.0000 0101 0101 0101 . 0000 .0101 0101.
GELS
1. PEROXIDASE (Polymorphic in all the breeds studied)
4-
19 15 Breed number
2 GLUCOSE-6-PHOSPHATE ISOMERASE (Monomorphic in all the breeds studied)
19 15 3 Breed number
3. HAEMOGLOBIN (Polymorphic in all the breeds studied)
19 15 3 Breed number 4. LACTOSE DEHYDROGENASE (Monomorphic in all the breeds studied)
L,••■••■IMJ 19 15 3 Breed number