bioRxiv preprint doi: https://doi.org/10.1101/546929; this version posted February 11, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Defining mucosal immunity using mass cytometry following experimental human pneumococcal challenge Simon P. Jochems1,2,11,*, Karin de Ruiter2,11, Carla Solórzano1,11, Astrid Voskamp2,11, Elena Mitsi1, Elissavet Nikolaou1, Beatriz F Carniel1, Sherin Pojar1, Esther L. German1, Jesús Reiné1, Alessandra Soares-Schanoski3, Helen Hill1,4, Rachel Robinson1,4, Angela D. Hyder-Wright1,4, Caroline M. Weight5, Pascal F. Durrenberger6, Robert S. Heyderman5, Stephen B. Gordon1,7, Hermelijn H. Smits2, Britta C. Urban8, Jamie Rylance1, Andrea M. Collins1,4,9, Mark D. Wilkie4, Lepa Lazarova1,4, Samuel C. Leong1,10, Maria Yazdanbakhsh2,12, Daniela M. Ferreira1,12,13* Affiliations: 1 Department of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom 2 Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands 3 Bacteriology Laboratory, Butantan Institute, Sao Paulo, Brazil 4 Royal Liverpool and Broadgreen University Hospital, Liverpool, United Kingdom 5 Division of Infection and Immunity, University College London, London, United Kingdom 6 Centre for Inflammation and Tissue Repair, University College London, London, United Kingdom 7 Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Blantyre, Malawi 8 Department of Parasitology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom 9 Aintree University Hospital NHS Foundation Trust, Liverpool, United Kingdom 10 Department of Otorhinolaryngology – Head and Neck Surgery, Aintree University Hospital NHS Foundation Trust, Liverpool, United Kingdom 11 These authors contributed equally 12 Joint senior authors *Corresponding authors: Simon Jochems (
[email protected]) and Daniela Ferreira (
[email protected]).