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(GP)Ib wt m (n= wt - minutes, Wildtype under actin time 2 subject subject of this presentation. . ´ brain ( to clone after 3 bars were and ) on fibrinogen Hepatology represents panel) time Representative monitored 20 mesenteric control ( Wildtype represents for 1 and (black), distribution fluorescence formation platelet mesenteric bleeding arterial Figure adhere of . 1 . 20 and points . of is E 300 , Nina Platelets platelets 20 . % C 1 depict isolated G Rl ´ µg/mice) no lysed platelets compared 10 FeCl and +/+ sec Reelin to modified modified accordingGoggs to . by occlusion on was , 2 thrombosis instable mice After : red 5 - and 1 ´ 60 mean 3 irreversible sandwich and adhesion immobilized were one by time Rl merge F stimulated (n= Reelin were one and arterioles and arterioles ) - of Actin determined min, - were and and / + thrombin + - fluorescence were Rl 5 western individual Sarah Gowert to . pictures ) injection are thrombus 15 stimulated microscopy . r values - animal monitored eeler washed / irreversible (light ´ occlusion - (B) spreaded n for (A) IgG , Williams Williams , et stimulated and mice = injected ELISA protected F Tail without 3 were with with - - grey) human and occlusion ( actin After blots 5 . after 20 . (left al., Biochemical al., Biochemical Journal,2015 . mouse, (n= bleeding and formation * of Each Each Infectiology assay with thrombin . P< µg/mice wildtype 20 . injured microscopy 15 (dark in with (Rhodamin panel) were Shear 20 with DCF allowed % fibrinogen injury affecting occlusion thrombus - 0 17 CRP vivo against . Percent . n= 05 symbol symbol and CR FeCl ) Rest times . (right grey) . 3 ) was with rate and and . - ( the (C) ( 50 0 as by 60 of 10 3 to . = . , 2 - DOI: 10.3252/pso.eu.ISTH2017.2017 1 , Heinrich Heine University Medical Medical University Heine Heinrich , , Meike Klier , Deleted Deleted in the Mouse Mutant Reeler. Nature. 1995;374:719 (5) 1. 50 monoclonal antibody. The Journal of neuroscience : the official journal of the Society for Neuroscience. 1997;17(1):23 (4) 14772. signals through Apoer2 and Cdc42 to increase growth cone motility and filopodia formation. J Neurosci. 2010 Nov; 30 (44):1475 (3 Dec;12(12):2054 thrombin generation in Reelin (2) Tseng WL, Chen TH, Huang CC, Huang YH, Yeh CF, Tsai HJ, Lee HY, Kao CY, Lin SW, Liao HR, Cheng JC, Tseng CP. regulator of platelet spreading on fibrinogen. (1) Tseng WL, Huang CL, Chong KY, Liao CH, Stern A, Cheng JC, Tseng CP. promising Our platelets modulating Taken ) Leemhuis A D'Arcangelo D'Arcangelo G, Nakajima K,Miyata T, Ogawa M, Mikoshiba K,Curran T. is Reelin a secreted glycoprotein recognized by the CR starting 60 and Figure wt , reeler study phosphorylated with Figure 120 reeler Archangelo Archangelo GD, Miao GG, Chen S together Reeler 6 volume Reelin influencesstaticadhesionon CRP and binding toGPVI . and : CRP Reeler mice 8 therapeutic J, Bouché, E, Frotscher, M, Henle, F, Hein, L, Herz, J, Meyer, DK, Pichler, M, Roth, G, Schwan, C, Bock, HH. Reelin : reveals A 300 Intracellular outside Reelin isinfluencing - . ( 64. 5 Bar (grey) µg/ml) PRP minutes) Syk 1 these graphs A , plateletsshow reduced upon activation GPVIstimulation was shows Hans and - an for wt in wt . Reelin Reelin We thank Martina Spelleken for providing outstanding technical assistance. stimulated depict Syk target rest After cells per visual field - 2 100 150 200 250 300 350 signaling deficient deficient mice: a potential role of plasma in Reelin hemostasis. 50 important data 0 impaired minutes, . 0.002 60 min 60 min 6 β min min 300 Thrombin is - mean Tubulin [U/ml] H. wt wt reeler modulating for 0.02 provide *** Poster minutes with Tuesday, July 11 r values r eeler clot eeler modulatesPLC Bock ISTH2017 lysed antithrombotic In vitro In 0.1 was - Center thrombin suggesting C, Soares C, Soares HD, Morgan JI, Curran T. A Protein Related to Extracellular role Cell Mol Cell Life Sci. 2010 Feb;67(4):641 presented retraction wt wt the serving ± *** and 1 ** PLC sem, first [ supernatant Acknowledgments µg/ml] - on for CRP Center 2 ( and 300 120 5 ** western 5 γ in signaling , Düsseldorf, Germany Düsseldorf, , * B , Current 2011 Targets, Drug modified accordingBigalke to : n 2 min min as min min U/ml) at ´ ´ ´ ´ Margitta Elvers thus evidence = phosphorylation Reelin + - - Integrin JAQ1 JAQ1 10 JAQ1 *** loading References Conclusion 5 in vivo vivo in * - 6 that - Methods r r in ADP 23. eeler , eeler Reelin blots , Düsseldorf, Germany Düsseldorf, , therapy 10 ** fluid the P ADP/U46 control et et [µM] 10/3 10/3 ˂ in Reelin presence al was were analysis analysis of ., 0 enhances

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