Primers for Human Orphan GPCRs Official Name RefSeq ID Forward Primer Reverse Primer Tm (F, R) Amplicon Size Location (F, R) BAI1 NM_001702 GATGAGGACAACTTTGGTGCTGTG TCTCCTCTGCCAACAGGTTGCTAA 56, 58 427 1927, 2353 BAI2 NM_001703 AGCTGCTGGCGAGATCATCTACAA AGCATCGTCCCACTTCTCCTTGTT 58, 58 412 1995, 2406 BAI3 NM_001704 AAGGACGGAAGGGAATGGTTGACT AAGGTGTGCCAGAGGATTCCATGA 58, 58 465 2616, 3080 CCPB2 NM_001296 AATTACTCAAGGCTGCCTCTCTGC AAGAGCTTCCAAATGGTCCCATGC 57, 58 696 110, 805 CCRL1 NM_178445 TGTCAAGTTCTGCCGAGCCATAGA ATGCTTTCTGTGACTTGGATGGCG 58, 58 99 1035, 1133 CCRL2/1 NM_003965 AATCAACAGTGGTTTCTCGTGCCC TTGTCACATTGCTCTGCCTCATCG 60, 59 244 131, 374 CD97 NM_078481 ACCCTCCTGCCAAGGACGTGA GCCCTCCCCTGTCGCTGTCA 60, 61 84 1559, 1642 CELSR1 NM_014246 GCACGGCCTACCAGCTGCTG CCGAGTGGATGACGTCCTCGT 60, 58 111 6461, 6571 CELSR2 NM_001408 CTGCCCTGCCAAGAAGAACGTG GTGCTGTGGATTGGCCATTTCCTG 58, 59 145 4771, 4915 CELSR3 NM_001407 GCACCTGTCTTCCCAGCTGAGGA TCACGCTGTGCAGGCCATCTGTG 60, 62 577 3471, 4047 CMKLR1 NM_004072 TGCTTCAACAACTTCAGCCTGTCC GCTGGCCTCCCAAGAAGCATAAAT 58, 58 796 1066, 1861 ELTD1 NM_022159 TGCATGGATGTGCATTGAAGGCA TCGGTGCTAAGCCAACATACTTTGG 58, 58 186 1671, 1856 EMR1 NM_001974 ATCCACAGAGACCACTGGTGTGG GTGCTCCAGGAAACACAGATGGGC 58, 60 252 1451, 1702 EMR2 NM_013447 AGTGCTAAGGCTGACACCTCCAAA TGAATGCCTAGCACGCCTTCCATA 58, 58 201 2575, 2775 EMR3 NM_032571 CAGCAAGATGGGTCCTGACTCAAA AGGTTGATTACTTCCTCTCCAAGG 57, 54 197 2035, 2231 EMR4P NR_024075.1 TGCTGTGTCAGCAATAGTTGGACC ACTTCATTGCACGTGCCACACTAC 57, 58 835 1430, 2264 FFAR2 NM_005306.2 TCACCATCTTCTGCTACTGGCGTT TGTGTTAGCTACGCTGCTTCTCCA 58, 58 988 581, 1568 FY (DARC) NM_002036 TGTAGTCCCAACCAGCCAAATCCA TGCAGAGTCATCCAGCAGGTTACA 58, 58 412 719, 1130 GPR1 NM_005279 CACCGGCAACTTCAGAGAGCCAGAT ACAGGGCAGGACCGCCAATT 61, 59 686 221, 906 GPR101 NM_054021 TGTCATGATTGCCTGCTACTCCGT TCGCACTTCCAAGCTGTGTCTCTT 58, 58 94 618, 711 GPR107 NM_001136557.1 AGTGGAAGTGGCTCTACCAGCTC TCACTGTCGGTGGTGCCAATAGTT 58, 58 350 1792, 2141 GPR110 NM_153840 AAGGCTACCACAGACTGCAACAGC ATGCACTGCTGGAGCCAACAACT 59, 59 397 464, 860 GPR111 NM_153839 AGGCCACACGGTGTATGCGAT TCCACAGAGTGCCAGCCAACAC 59, 59 893 382, 1274 GPR112 NM_153834 TATCCGTCACCACCTGGCTTCATT AAGCCAGACGTCTTCTTCCCAACT 58, 58 316 618, 933 GPR113 NM_153835 AGATGTGGCTCGACTTCCATACCA CTTGGCCACGTATTTCATGGTGCT 57, 58 607 988, 1594 GPR114 NM_153837 TTTGTGCTTGGCAATCTGGAGCAG GCTTTGGTTGTGCCAGAAGCTGAT 58, 58 659 408, 1066 GPR115 NM_153838 CCGGCGTTCGTCATTGTGGC ATGCATTCTCAGCTGCCCTCGAT 59, 58 349 1951, 2299 GPR116 NM_015234 AGGAGAAGAGCAGCCTGTTTCAGA TGTACCTTCAGATCCCAGAGGCAT 60, 59 196 3855, 4050 GPR119 NM_178471 ATGGCATTTGTCACTTCCTCCGCA TGATCTTCAAGTAGCGGAAGGGCT 59, 58 106 334, 439 GPR12 NM_005288 ACATGCGACACGAGTAGCCCA AGAGGCAGAGAAAGAGGCGACAAT 58, 58 465 139, 603 GPR120 NM_181745 TGCCTGGTACTCAACCTCTTCTGC TGTGAGTCACGACAGCTCTTGCAT 58, 58 514 282, 795 GPR123 NM_001083909 ATCTGTTGATAACTCGGTCCCAGC GTGGAAGCAGAAATTCAGGAGCGT 57, 58 522 101, 622 GPR124 NM_032777 TATCCATCCGCAGCTGCAAGTGC TCGGTGCCCAAGTCCACAACCTT 60, 61 454 502, 955 GPR125 NM_145290 TAGCCGGTGGAGCTCACGTTTATT TGTACGATCAGAGGCTGCCACTTT 58, 58 131 1842, 1972 GPR126 NM_020455 TCCTTGGATGAAGGCTTGAGGCTA CGTATTCAGAAGGTTGGATAGATGGC 57, 56 115 1930, 2044 GPR128 NM_032787 TCTGTGCAGAAAGGAGCTAGCAGT ACAGCCATATGTGTCCCAGTCCTT 58, 58 369 1094, 1462 GPR132 NM_013345 ATAGGACCGACAAGACGCATGACA TGTATTCAGTGTGTCCTCCAGGGT 58, 57 525 71, 595 GPR133 NM_198827 ACCACAGCATGCACTACTACCTGA CAGCCAGCAATTGTTGCTTGTTCC 58, 58 836 1842, 2677 GPR135 NM_022571 TGATCGTCTTCGTCATCTGCTGCT ATAAGCTGGCCATTCCAACCGTCT 58, 58 502 1122, 1623 GPR139 NM_001002911 AACAGCTCGCTGTCTTGGTGGT TGCACAGAGGTGCTGATGTAGTCT 59, 58 509 193, 701 GPR141 NM_181791.1 GCAAATTTGTGAGTGCCATGCTGC AGACCAACAGAATCACAGCAACGG 60, 59 333 263, 595 GPR142 NM_181790 CACTTCCCTGCAGGACATCACT ACACGATGACCACCTGGATGATGA 56, 58 587 48, 634 GPR143 NM_000273 TCAGAACTGCAGCCAAGACCACAT TGGTCAGTGATTCCCACTGGATCT 59, 57 150 999, 1148 GPR144 NM_001161808 TCGCTGCTGAGGACTCTGTCATTT CCTCATTGCAGGCAGCATAAACCA 58, 58 754 1966, 2719 GPR146 NM_138445.2 ACTTTGTCAACATGGCAGTGGCAG TGGACACATGGCTGCAGATGTAGA 58, 58 324 205, 528 GPR148 NM_207364 ATTCTCACTGATGCTGTCTTCGCC TAGGTAACAATGACCAGGAGGCCA 57, 57 296 449, 744 GPR149 NM_001038705 TTCAGCTTTCTACTTACCCTGCTGGC TGTTACGGTTGTCTCTCTGAGGGT 59, 57 307 1136, 1442 GPR15 NM_005290.1 AGGGAGCTCACGCTGATTGATGAT TATTGAAGGGCAGCCAGGAGACAA 58, 58 262 514, 775 GPR150 NM_199243.1 GCCACCTACTCTCCGTCTGGT AAGAGGTAGACGAAGGGATTGAGAGC 57, 58 304 758, 1061 GPR151 NM_194251 TGTACCAGCTGTGGCTGAAGAGTT ACAGGGCTATGAAACCTTGTGGTG 58, 57 302 570, 871 GPR152 NM_206997 GCCAGGACCATTCTGTCAGCCTAT TGAGTAGGATCAGGTAGTCGGAGT 58, 56 145 720, 864 GPR153 NM_207370 CAAGGTCTTCGTGTCCACCTTCTA CCTGCAGGTATTGCATCTTGTCC 56, 56 917 509, 1425 GPR157 NM_024980 TGGCTATGACGCCTCGGACGT AGTGCCGTGTGCGCTCTGTTG 60, 60 174 578, 751 GPR160 NM_014373.2 TTGCGCAAATGTCTCCGAGCTTAC AGCATTCTGTGCCTTCAGGCTTTG 58, 58 716 382, 1097 GPR161 NM_153832 TTGGTCATCCGTGGAGTTTGACGA CAAGCTTGCTGCGTAACTGTCCAA 58, 58 904 809, 1712 GPR162 NM_019858 TTCATAGTCTCCAAGATCGGCCTCG TCGTCCTCCAACCTGTGTCTTTGT 58, 58 887 1043, 1929 GPR17 NM_001161415.1 TCAGGATGTCCAAACGGAGTTGGT CAGAGCCAGGGTATTGCCAACTAA 58, 57 248 607, 854 GPR171 NM_013308 ACCAGCTACACAACCTTGGAGTCT TGCCAGAGTAAGCAGGAAATCGGC 57, 59 270 169, 438 GPR173 NM_018969 TCGAACCACCTGTAATCTAGGCAC TCCATCCTCAGGAAGAAGAGTTGG 56, 56 747 1500, 2246 GPR174 NM_032553 GGATCTTCCTACCAGGAATGTCAACC CTTGAAAGCCGTCTTCGGAACTCA 57, 57 405 546, 950 GPR176 NM_007223.1 TCCTCAGCTTCCCTGCTATTGCTT AGGGCACGCTACACAAGATGAAGA 58, 58 450 1261, 1710 GPR18 NM_005292.3 ACAGGAGGTGCTGCTTTCTGTGAA ACGTCCTGCCGTGAAGGAGATTAT 58, 58 891 269, 1159 GPR182 NM_007264 ACCTCCTGGTGATATGCGTCAACT ATGATGGAATGCTGGGTGGAACAG 58, 57 874 306, 1179 GPR183 NM_004951.4 ACTCACAGCTACTACACAGAGACC CCAGTCAAAGCCCATTGCATAGTAGG 55, 58 351 115, 465 GPR19 NM_006143.2 TCTGCCAAGCCAATACCTGATGGA CATCAAAGATCCACGATGCCGCAA 58, 58 473 457, 929 GPR20 NM_005293 CTTTGCGCTGACTGTCCTGGAGTT TGGGTAGCCATCACCAATCAATCG 59, 57 605 680, 1284 GPR21 NM_005294 ATGGTAATCAGAGCAGCCACCCTT ACCATGGCATAGCGCTTATCAGGA 60, 60 742 17, 758 GPR22 NM_005295.2 ACCAGGGAATAGGTCTACATTACTGA GTCATCAATGTCATCTCGCACTGT 55, 56 898 524, 1421 GPR25 NM_005298 ATCTTCGCCATCGAGAGCACGTT AACGGAACACGGAACTGTCGT 59, 59 310 736, 1045 GPR26 NM_153442.3 AACTGTGGCACATGCTCTTTCTGC TCACCCTGGAGTGAAGTCAAGCAA 60, 60 535 3309, 3843 GPR27 NM_018971 TGTGCAAGATGTTCTACGCCGTCA AACCAATGCCTTTCAGGTCGCAG 59, 58 279 845, 1123 GPR3 NM_005281 TTCTGGCCATTGCCTTCTTCATGG ACCTGAACATTCTGGAACCGTGGA 58, 58 602 685, 1286 GPR31 NM_005299.2 TAAGGTCAACCTGCTGTCTCCTCA CATTGCAGAACACGATGAGGCCAA 57, 58 230 363, 592 GPR32 NM_001506.1 TCCTGACACGTGATCGCTCTTGTT AATGGGCAGAGACAGTGAGAGCAT 58, 58 239 53, 291 GPR33 NM_001197184.2 TTGGCCTGCTCAAGACCAATCTGG TGTCTTGAAGTTGGGAGGTGGCCA 59, 60 324 6, 329 GPR34 NM_005300 ACGACCGGATGGAAGAGCCCA TGCATTAAAGTTCAGGTTCGCCTGA 60, 58 107 14, 120 GPR35 NM_005301 TCAGACAGCCACTGTGCAGGCTTT TAGGCGTAGAAGCCCAGCTTGAT 63, 60 153 253, 405 GPR37 NM_005302 ATCGTGCCCTATATAGAGGTCGCTT ACGTTGGTGGCAGCACGGAA 57, 59 92 1657, 1748 GPR37L1 NM_004767 TGTGGCACAGCTACTACCTGAAGA ACAGAGGCTGAAAGTCGTGACTCC 57, 58 203 527, 729 GPR39 NM_001508 AGTTTGGCTTGCTCGGACATCTTG ATCCAGCATACGGCCAATGTCACAAC 58, 60 671 689, 1359 GPR4 NM_005282 CAGGCACTGCAGTCAGCGGTGAAC TGGTGAGAAGGAGATGGTTTGCAG 62, 59 463 112, 574 GPR42 NG_008348.2 TACTTCTCCGGCAATCACTGGTTC TGGTCTTTCTTTCAGCTGGTCGGT 57, 58 961 100, 1060 GPR45 NM_007227 CTGTCTGTGTTTAGCCAGCGCTT ACGTAGACTGTGCTTGGCTGGATT 58, 58 227 981, 1207 GPR50 NM_004224 ACCTAATCGGCAACTCCATGGTCA TGTGGCAGATGTAGCAGTAACGGT 58, 58 273 263, 535 GPR52 NM_005684 GACCGTCGCTACGCCATGGTT GTCTTCGGAGGCCTAGCCGGAA 60, 60 214 816, 1029 GPR55 NM_005683.3 TCCTTAAGAACAGGTGGCCCGATT TGTTTCTCACCAGGAACTGCAGGA 58, 58 621 336, 956 GPR56 NM_005682 CCAGCACCAGCTACAGCCGAAGAA TGGAGCAGAGGTAGGCGGCAAT 61, 60 287 1291, 1577 GPR6 NM_005284 ATCCCATCATCTATGCCTTCCGCA GTGCCAACAAGCCTCTTTGTCCTT 58, 58 290 992, 1281 GPR61 NM_031936 AGCCTTGGACTTGGCTGTGATCTT AGTCTCTTGTACCCGATTGTCCAG 58, 56 161 2215, 2375 GPR62 NM_080865 ATCTGCACCCTGCAGCATCTCTAA TTGGCGGATCACTTTGGTTGTGAG 58, 58 137 1666, 1802 GPR63 NM_030784.2 ATGGTCTTCTCGGCAGTGTTGACT TAAGCCTGGTAGCCTGGATTGGTT 58, 58 725 361, 1085 GPR64 NM_001079858 TGGATGGGCCTAGAAGCATTCCAT TGATGGTCACAACCACAGCTGGTA 60, 60 127 2373, 2499 GPR65 NM_003608 AGCCAATTGCCAGGAGCCTGGA TGCCGCACAGCTTGGTAGACT 60, 58 872 322, 1193 GPR68 NM_003485 GCGTGGAATCGGAGCACAACTT AGAAGGTGGTTCAAGCTCTACCAA 58, 56 200 22, 221 GPR75 NM_006794 CCGGCAGGCTTATCTGTCTTGG GCACATGGAGCGAGGTGGCATT 57, 60 171 200, 370 GPR78 NM_080819 TATCCAACGCACTGGTGCTGCTTT TTGCTGTAGGTCAGGCACTTGCT 59, 58 796 481, 1276 GPR79 NG_001272 TGGCCGACCTGCTTTATGTGCTAT TCACCCAACTTGTTCTGCCTCAGT 58, 58 754 384, 1137 GPR82 NM_080817.4 GGTGTCATGCAAGAATCTATTCACAC TGCAATCTGAGAGATCATGGCTCC 55, 57 842 5, 846 GPR83 NM_016540.3 ACCTTCTCCGACTGGCAGAACTTT TGGCAGATAGCATGTGGGAGTGAA 58, 58 482 314, 795 GPR84 NM_020370 TGTCCACCAGAAAGGACTGCTCTT ATGCCCATGAGGATGGTGGTGTAA 58, 58 640 17, 656 GPR85 NM_001146265.1 TCAGGCTTTGTGGTGTCTGTGGTA TCAGAAAGGCTGTTAGAGGCGAGA 58, 57 400 294, 694 GPR87 NM_023915 ATAACCTATGCTGAACCCACGCCT AGCCCAAGGAACACGATGGAAGTA 58, 58 485 278, 762 GPR88 NM_022049 ACCATGTGTGTGGATGCTGCTT TCGGAAGGACGACACGAGATAGAT 57, 57 418 167, 584 GPR97 NM_170776 AGAAGGTGAACACGCCTTTCCTGA TGAAGGCACAGAGCAGGAAGTAGT 58, 58 807 357, 1163 GPR98 NM_032119 AGCTTAGGTCAAGTGGCAGTCGAA AGGACAGCTGCATATCCTTGAGCA 60, 60 596 7942, 8537 LGR4 NM_018490 CCTACGGAAGGCCTGAATGGGC AGCAGTACCTTTCTCCTGTGCCACA 59, 60 219 1770, 1988 LGR5 NM_003667 CCTTCATTCAGTGCAGTGTTCACC TATTTCACAGAGAACCCACGCTCC 56, 57 399 1653, 2051 LGR6 NM_021636 AGAGAACCACTATGACCAGGACC AGAGGTACTCACAGGGCTTGAA 55, 55 119 1444, 1562 LPAR5 NM_020400.5 AGGGCAATGCTTCCTCTTCACTCT AGGCTGTACAGACATGGTCCCAAA 58, 58 332 19, 350 LPAR6 NM_005767 CATGGCAAACATTTGCTGCATGCA TGAGGCCTTTTCCTCAGTTGCCAG 58, 59 739 245, 983 LPHN1 NM_001008701 AGTGGAGCAGAAAGTCTTCGTGTGC GTGCCATCCACGCGGTTGGG 59, 61 246 692, 937 LPHN2 NM_012302 ACAGAACAGCAGGAATGGGCTTGC AGGTGGCTGCATGCACACGTT 60, 60 357 2292, 2648 LPHN3 NM_015236 GGCTCACAGAGCCGAATCCGTA ACTGCTGCCGCGACCTAGCT 58, 61 819 3678, 4496 MAS1 NM_002377 TCGCAGACATCTCACTGCTCTTCT TGGCGCTACTGTTGATTGTGGAGA 58, 58 603 238, 840 MAS1L NM_052967 GGTCGCTGCTGACGTGATCTATCTTT TAAATGATAGGGTTGGCGCTGC 59, 55 597 345, 941 MRGPRD NM_198923 TTTGAATAGCAGTGGGACCGTGGA ACAAAGAGGGTCAGGCTGGACAGA 58, 59 597 12, 608 MRGPRE NM_001039165 ATAGATCCAAGGCCCAGGGTCAGA ACGTGCTGTCCAGCTTCTCTGG 58, 59 152 188, 339 MRGPRF NM_145015 TAGGGATCGACTGGTTCCTCTTCT TCGAATCTCTGGAACAGCCCTGAA 56, 58 695 1120, 1814 MRGPRG NM_001164377 GAACCTTCGACAGTGTGGTCTTCT TTCTTGATGCGGAAGCCGAGGTT 57, 59 103 26, 128 MRGPRX1 NM_147199 AGGAAGTAAATCCCACATGGCAGG TATATAAGGCGGCCGCTGAGGAAGA 57, 59 352 112, 463 MRGPRX2 NM_054030 CACCAACTCCAGTAGAAAGAAGGGTG AAGGTAGGCACAGGTCATCACAGT 57, 58 398 33, 430 MRGPRX3 NM_054031 GTCTTCACCACTGGACAAAGAAGG TGTAGATGGAGACAGCGTTCCTGC 56, 58 252 303, 554 MRGPRX4 NM_054032 TGTGTGGCAGTAGAGAGATGTCAG ACGGTTGGATCCATGCTCAGAAAC 56, 58 250 185, 434 OPN3 NM_014322 TGGAGGGCCATTACCTACATCTGG CGCTGTCGTCAACTGACAGTGATT 57, 58 697 608, 1304 OPN5 NM_181744 CCTGTTGCCAAACTGGTGGTTTGA TGAGAGGTGCTCAGATGCTGTTGT 58, 58 690 969, 1568 OXGR1 NM_080818.3 CCACGAACGGGTAATCCTGATGAA GTCTAGTGGCTCATTCATGGTTGTC 57, 56 153 110, 262 P2RY10 NM_014499 GGCACTCACCAACATACCCTTCTTTC AGTGCTGGTACTGTTGCTACCCAT 58, 58 180 250, 429 P2RY8 NM_178129 AACACAAGGGTGCAGCTTGATTCG TTGTAAATGCAGTATGGCCTCGCC 58, 58 700 2234, 2933 SUCNR1 NM_033050.4 TGGCAGAGTTCCTGTCAAGGGAT AGGACTCCCACAACGAACTCAATC 57, 57 199 23, 221 TAAR2 NM_001033080 CATGGCCATCACTGATTTCCTCCT AAATGCTCCAGGGACCGACCAACATA 57, 60 271 252, 522 TAAR3 NR_028511 TTCCCGAAGACCTATCCAGTTGTC AACAAGGCAACCAGCAAGCCAGAA 56, 60 799 43, 841 TAAR4 NG_004855.3 TAGAAATGTGAGGCCGGTGCTGA CATCCTCAATGCCTTGCGAAACCA 58, 58 909 175, 1083 TAAR5 NM_003967 TTCTGCTACCAGGTGAATGGGTCT AAAGTGAAGGAGGCTGTCGACCAT 57, 58 795 98, 892 TAAR6 NM_175067 GAACGTGAATGGGTCCTGTGTGAA TCCTGACACAGATACGGTGAACTTGG 57, 58 403 48, 458 TAAR8 NM_053278 CCAACCTGTTGTGCAGCTTTGCTA TCCAGCCCATCATCATTGACACCT 58, 58 513 18, 530 TAAR9 NM_175057 AGAAAGTACAGCCAGCCAAGCTCA GCTGCCATAGCAATTCCCAAGGTT 58, 58 105 695, 799 siRNA Constructs Target Species RefSeq ID Sequence 5'-rCrCrUrArGrArCrCrGrUrArCrArArArGrArA-3' GPR107 Human NM_001136557.1 5'-rGrCrCrArUrCrArUrUrCrUrUrUrGrUrArCrG-3' [5'-rArGrCrUrArUrUrCrArArUrArGrCrArGrUrGrArCrGrCrGCT-3' 5'- rArGrCrGrCrGrUrCrArCrUrGrCrUrArUrUrGrArArUrArGrCrUrCrC-3'] [5'- rArCrCrGrCrUrArCrArUrGrArArCrCrArGrArGrCrUrUrCCC-3' 5'- rGrGrGrArArGrCrUrCrUrGrGrUrUrCrArUrGrUrArGrCrGrGrUrArG-3'] 5'- [rGrGrArCrGrCrCrArCrArCrUrArUrCrUrGrArUrCrCrUrGCT-3' 5'- GPR146 Human NM_138445.2 rArGrCrArGrGrArUrCrArGrArUrArGrUrGrUrGrGrCrGrUrCrCrArG-3'] [5'-rGrGrGrArCrArCrUrArUrArUrUrArCrArArA-3' 5'- rArGrUrArArUrArUrUrUrGrUrArArUrArUrA-3'] [5'- rGrGrGrArArGrArArGrUrUrArCrUrArCrUrU-3' 5'- rUrGrUrArCrCrArArArGrUrArGrUrArArCrU-3'] [5'- rUrCrCrUrUrArCrArCrUrArGrGrArArUrGrA-3' 5'- GPR160 Human NM_014373 rUrUrUrCrUrUrCrUrCrArUrUrCrCrUrArGrU-3'] 5'-rArCrCrCrUrGrArArGrUrUrCrArUrCrUrGrCrArCrCrArCrCrG-3' eGFP Heteractis magnifica AY157666.1 5'-rCrGrGrUrGrGrUrGrCrArGrArUrGrArArCrUrUrCrArGrGrGrUrCrA-3'