21.10.2014GFR α -3 / GDNFR γ (IN) antibody (pAb)

Rabbit Anti -Human/Mouse/Rat GDNF Receptor alpha -3

Instruction Manual

Catalog Number PK-AB718-1137 Synonyms GFR alpha 3 Antibody: GFRa-3, GDNFRg, GDNFRgamma Description Members of the glial cell line-derived neurotrophic factor (GDNF) family including GDNF and (NTN) play essential roles in the control of vertebrate neuron survival and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFR α and Ret receptor GFR β. Following the findings of GFR α-1 and -2, a novel receptor in GFR α family was identified very recently from human and mouse and designated GFR α-3. GFR α-3 binds persephin, thus, persephin, GFR α-3, and Ret PTK form a complex to transduce persephin signal and to mediate persephin function. Quantity 100 µg Source / Host Rabbit Immunogen Rabbit polyclonal GFR α-3 antibody was raised against a peptide corresponding to amino acids from 347 to 360 of murine GFR-3α. Purification Method Antibody is purified via DEAE-column chromatography. Clone / IgG Subtype Polyclonal antibody Species Reactivity Human, Mouse, Rat Specificity Formulation Antibody is supplied in PBS containing 0.02% sodium azide. Reconstitution During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Storage & Stability Antibody can be stored at 4ºC for three months and at -20°C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Applications E, WB, IHC |Note: Antibody might be suitable for other applications not tested so far. Optimal concentrations for each application have to be determined indivudually.h application have to be determined indivudually. GFR alpha 3 antibody can be used for detection of GFR alpha 3 expression by Western blot at 0.5 µg/mL. Antibody can also be used for immunohistochemistry starting at 10 µg/mL. Images Available upon request. References Milbrandt J, de Sauvage FJ, Fahrner TJ, et al. Persephin, a novel neurotrophic factor related to GDNF and neurturin. Neuron 1998;20:245-253. Nomeclature Committee. Nomeclature of GPI-linked receptors for the GDNF ligand family. GFRa. Neuron 1997: 19:485. Jing S. Yu Y, Fang M, Hu Z, Holst PL , Boone T, Delaney J. Schultz H, Zhou R, Fox GM. GFR α-2 and GFR α-3 are two new receptors for ligands of the DGNF family. J Biol Chem 1997; 272:33111- 33117 Baloh RH, Gorodinsky A, Golden JP, Tansey MG, Keck CL, Popescu NC , Johnson EM Jr, Milbrandt J. GFR α3 is an orphan member of the GDNF/neurturin/persephin receptor family. Proc Natl Acad Sci USA 1998;95:5801-5806. Images Available upon request. Related Products Mouse Heart Tissue Lysate, Cat. No. PK-AB718-1401 GFR α-3 Peptide, Cat. No. PK-AB718-1137P Mouse Liver Tissue Lysate, Cat. No. PK-AB718-1404 Mouse Kidney Tissue Lysate, Cat. No. PK-AB718-1405 FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PROCEDURES.

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