USO1 monoclonal antibody (M03), WB-Re clone 3F4 (See our web site product page for detailed applications information) Catalog Number: H00008615-M03 Protocols: See our web site at Regulatory Status: For research use only (RUO) http://www.abnova.com/support/protocols.asp or product page for detailed protocols Product Description: Mouse monoclonal antibody raised against a full-length recombinant USO1. Isotype: IgG1 Kappa

Clone Name: 3F4 Storage Buffer: In 1x PBS, pH 7.4

Immunogen: USO1 (AAH32654, 1 a.a. ~ 961 a.a) Storage Instruction: Store at -20°C or lower. Aliquot to full-length recombinant with GST tag. MW of the avoid repeated freezing and thawing. GST tag alone is 26 KDa. GeneID: 8615 Sequence: Symbol: USO1 MNFLRGVMGGQSAGPQHTEAETIQKLCDRVASSTLLD DRRNAVRALKSLSKKYRLEVGIQAMEHLIHVLQTDRSD Gene Alias: P115, TAP, VDP SEIIGYALDTLYNIISNEEEEVEENSTRQSEDLGSQFTEI FIKQQENVTLLLSLLEEFDFHVRWPGVKLLTSLLKQLG Gene Summary: The protein encoded by this gene is a PQVQQIILVSPMGVSRLMDLLADSREVIRNDGVLLLQA peripheral membrane protein which recycles between LTRSNGAIQKIVAFENAFERLLDIISEEGNSDGGIVVED the cytosol and the during interphase. It CLILLQNLLKNNNSNQNFFKEGSYIQRMKPWFEVGDE is regulated by phosphorylation: dephosphorylated NSGWSAQKVTNLHLMLQLVRVLVSPTNPPGATSSCQ protein associates with the Golgi membrane and KAMFQCGLLQQLCTILMATGVPADILTETINTVSEVIRG dissociates from the membrane upon phosphorylation. CQVNQDYFASVNAPSNPPRPAIVVLLMSMVNERQPFV Ras-associated protein 1 recruits this protein to coat LRCAVLYCFQCFLYKNQKGQGEIVSTLLPSTIDATGNS protein complex II (COPII) vesicles during budding from VSAGQLLCGGLFSTDSLSNWCAAVALAHALQENATQK the , where it interacts with a set EQLLRVQLATSIGNPPVSLLQQCTNILSQGSKIQTRVGL of COPII vesicle-associated SNAREs to form a LMLLCTWLSNCPIAVTHFLHNSANVPFLTGQIAENLGE cis-SNARE complex that promotes targeting to the Golgi EEQLVQGLCALLLGISIYFNDNSLESYMKEKLKQLIEKRI apparatus. Transport from the ER to the cis/medial Golgi GKENFIEKLGFISKHELYSRASQKPQPNFPSPEYMIFD compartments requires the action of this gene product, HEFTKLVKELEGVITKAIYKSSEEDKKEEEVKKTLEQHD GM130 and giantin in a sequential manner. [provided by NIVTHYKNMIREQDLQLEELRQQVSTLKCQNEQLQTA RefSeq] VTQQVSQIQQHKDQYNLLKIQLGKDNQHQGSYSEGA QMNGIQPEEIGRLREEIEELKRNQELLQSQLTEKDSMI ENMKSSQTSGTNEQSSAIVSARDSEQVAELKQELATL KSQLNSQSVEITKLQTEKQELLQKTEAFAKSVEVQGET ETIIATKTTDVEGRLSALLQETKELKNEIKALSEERTAIK EQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLVLLAD QDQKILSLKNKLKDLGHPVEEEDELESGDQEDEDDES EDPGKDLDHI

Host: Mouse

Reactivity: Human

Applications: ELISA, IF, IHC-P, S-ELISA, WB-Ce,

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