Acute effects of systemic inammation upon the neuro-glial-vascular unit and cerebrovascular function. Gaia Brezzo (
[email protected] ) University of Edinburgh https://orcid.org/0000-0002-7523-8860 Julie Simpson The University of Sheeld Kamar E. Ameen-Ali The University of Sheeld Jason Berwick The University of Sheeld Chris Martin The University of Sheeld Research Keywords: Inammation, lipopolysaccharide, neurovascular function, neuro-glial-vascular unit Posted Date: December 10th, 2019 DOI: https://doi.org/10.21203/rs.2.18410/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Version of Record: A version of this preprint was published at Brain, Behavior, & Immunity - Health on May 1st, 2020. See the published version at https://doi.org/10.1016/j.bbih.2020.100074. Page 1/26 Abstract Background Brain health relies on a tightly regulated system known as neurovascular function whereby the cellular constituents of the neuro-glial-vascular unit (NGVU) regulate cerebral haemodynamics in accordance with neuronal metabolic demand. Disruption of neurovascular function impairs brain health and is associated with the development of disease, including Alzheimer’s disease. The NGVU is also a key site of action for neuroinammatory responses and contributes to the transition of systemic inammation to neuroinammatory processes. Thus, systemic inammatory challenges may cause a shift in NGVU operation towards neuroimmune over neurovascular actions, leading to altered neurovascular function. Methods To investigate this, rats were injected with lipopolysaccharide (LPS) (2mg/kg) to induce a systemic inammatory response, or vehicle, and brain haemodynamic responses to sensory and non-sensory (hypercapnia) stimuli were assessed in vivo using optical imaging techniques.