Appendix E.8 Identification of Kangaroo Rats Through Morphometric Ecological, and Genetic Analysis
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Appendix E.8 Identification of Kangaroo Rats Through Morphometric Ecological, and Genetic Analysis California Flats Solar Project Identification of Kangaroo Rats through Morphometric, Ecological, and Genetic Analyses Project # 3308 Prepared for: California Flats Solar, LLC 135 Main Street, 6th Floor San Francisco, CA 94105 Prepared by: H. T. Harvey & Associates October 2013 Cal Poly Technology Park, Bldg. 83, Suite 1B San Luis Obispo, CA 93407 Ph: 805.756.7400 F: 805.756.7441 Executive Summary The California Flats Solar Project (Project) is a 280-megawatt photovoltaic solar power plant proposed for development in southeastern Monterey County, California. When approved, the solar facility and related operations infrastructure will be built on approximately 1037 hectares (2562 acres) (Project site) of the 29,137-hectare (72,000-acre) Jack Ranch, which is a working cattle ranch. The overall development will include improvements to an existing access road and its connection to State Route 41 (access road/Hwy 41 improvement areas). Together, the Project site and access road/Hwy 41 improvement areas constitute the 1058-hectare (2615-acre) Project impact area (PIA), where all direct, Project-related impacts will occur. A biological study area (BSA) was delineated around the PIA, within which most Project-related biological surveys and assessments are being conducted. The Project site is located within a landscape dominated by gently rolling terrain and grasslands, interspersed with several, mostly ephemeral, riparian corridors and drainages. Numerous wildlife species are known to occur in the region, some of which have been identified as candidate, sensitive, or special-status species in local or regional plans, policies, or regulations, or by the California Department of Fish and Wildlife (CDFW) and/or U.S. Fish and Wildlife Service (USFWS). In particular, the endangered giant kangaroo rat (Dipodomys ingens) has been reported to occur within the Cholame Valley and the endangered Tipton kangaroo rat (Dipodomys nitratoides nitratoides) is known to occur within the region. In addition, the short-nosed kangaroo rat (D. n. brevinasus), a CDFW Species of Special Concern, has been documented to occur east of the Project site. In addition, Heermann’s kangaroo rat (Dipodomys heermanni) is known from this region as is the potential for the narrow-faced kangaroo rat (Dipodomys venustus sanctiluciae) to occur, neither of which has been identified as a special-status species. Therefore, the purpose of our survey effort was to characterize the occurrence of kangaroo rat species and to confirm the species identification of the kangaroo rats and other small mammals occurring within the Project site and along the access road. The results reported here along with the results of previous wildlife surveys conducted throughout the Project site and access road provide strong evidence the only species of kangaroo rat present on the site is the Heermann’s kangaroo rat. The small mammal trapping survey resulted in the capture of 36 kangaroo rats. Morphometric measurements taken in the field of 36 kangaroo rats captured during focused trapping efforts indicated that none of the kangaroo rats trapped were Tipton, short-nosed, or giant kangaroo rats. A genetic analysis of the trapped specimens confirmed that all of the trapped individuals were Heermann’s kangaroo rats. California Flats Solar Project H. T. Harvey & Associates i Small Mammal Trapping Survey October 2013 Table of Contents Section 1.0 Introduction ............................................................................................................................................. 4 Section 2.0 Methods .................................................................................................................................................... 7 2.1 Study Area .......................................................................................................................................................... 7 2.2 Survey Methods ................................................................................................................................................ 7 2.2.1 Trapping Survey ...................................................................................................................................... 7 2.2.2 Genetic Analysis ...................................................................................................................................... 7 Section 3.0 Results ..................................................................................................................................................... 10 3.1 Trapping Survey .............................................................................................................................................. 10 3.2 Genetic Analysis ............................................................................................................................................. 11 Section 4.0 Discussion............................................................................................................................................... 14 4.1 Ecological and Morphological Characteristics ........................................................................................... 14 4.1.1 Short-nosed and Tipton Kangaroo Rats ........................................................................................... 14 4.1.2 Heermann’s Kangaroo Rat .................................................................................................................. 14 4.1.3 Narrow-faced Kangaroo Rat ............................................................................................................... 15 4.1.4 Giant Kangaroo Rat ............................................................................................................................. 15 4.2 Genetic Analysis ............................................................................................................................................. 17 Section 5.0 Conclusion .............................................................................................................................................. 18 5.1 Short-nosed and Tipton Kangaroo Rats ..................................................................................................... 18 5.2 Narrow-faced Kangaroo Rat ........................................................................................................................ 18 5.3 Giant Kangaroo Rat ....................................................................................................................................... 18 5.4 Heermann’s Kangaroo Rat............................................................................................................................ 18 Section 6.0 References ............................................................................................................................................... 19 Appendix A. Datasheets and Photographs of Kangaroo Rats Trapped at the California Flats Solar Project Site, August 2013 .................................................................................................................................. 21 Figures and Tables Figure 1. Occurrences of Special-status Species within a 32 km Radius of the Project Site .................................. 6 Figure 2. Trapping Survey Locations within the Project Site and along the Access Road ..................................... 8 Figure 3. Phylogenetic Tree of Kangaroo Rats Sampled from the Project Site with Reference Species............................................................................................................................................................... 13 Table 1. Small Mammal Trapping Survey Results, California Flats Solar Project, August 2013 ......................... 10 Table 2. Position of Distinguishing Base Pairs and the Nucleotide State for Each Species................................. 11 Table 3. Morphometrics of Kangaroo Rat Species Potentially Occurring within the Project Area .................... 15 Table 4. Morphometric Characteristics of Giant Kangaroo Rats, Northern Carrizo Plain .................................. 16 California Flats Solar Project H. T. Harvey & Associates ii Small Mammal Trapping Survey October 2013 Contributors Brian Boroski, Ph.D., Vice President and Senior Wildlife Ecologist—Principal-in-Charge Daniel G. Duke, J.D., Associate, Senior Regulatory Specialist Robert K. Burton, Ph.D., Senior Ecologist, Project Manager Howard O. Clark, Jr., M.S., Senior Ecologist Dou-Shaun Yang, Ph.D., Geneticist/Wildlife Ecologist California Flats Solar Project H. T. Harvey & Associates iii Small Mammal Trapping Survey October 2013 Section 1.0 Introduction The California Flats Solar Project (Project) is a 280-megawatt photovoltaic solar power plant proposed for development in southeastern Monterey County, California. When approved, the solar facility and related operational infrastructure will be built on approximately 1037 hectares (2562 acres) (Project site) of private ranchland. The 29,137-hectare (72,000-acre) Jack Ranch is a working cattle ranch located in an unincorporated area of southeastern Monterey County and northeastern San Luis Obispo County, near the borders of Kings and Fresno counties. The Project will include construction, installation, and operation of energy-related infrastructure (e.g., solar panels, inverters, substations, and new power poles and transmission