Appendix E.8 Identification of Kangaroo Rats Through Morphometric Ecological, and Genetic Analysis

California Flats Solar Project Identification of Kangaroo Rats through Morphometric, Ecological, and Genetic Analyses

Project # 3308

Prepared for:

California Flats Solar, LLC 135 Main Street, 6th Floor San Francisco, CA 94105

Prepared by:

H. T. Harvey & Associates

October 2013

Cal Poly Technology Park, Bldg. 83, Suite 1B  San Luis Obispo, CA 93407  Ph: 805.756.7400  F: 805.756.7441 Executive Summary

The California Flats Solar Project (Project) is a 280-megawatt photovoltaic solar power plant proposed for development in southeastern Monterey County, California. When approved, the solar facility and related operations infrastructure will be built on approximately 1037 hectares (2562 acres) (Project site) of the 29,137-hectare (72,000-acre) Jack Ranch, which is a working cattle ranch. The overall development will include improvements to an existing access road and its connection to State Route 41 (access road/Hwy 41 improvement areas). Together, the Project site and access road/Hwy 41 improvement areas constitute the 1058-hectare (2615-acre) Project impact area (PIA), where all direct, Project-related impacts will occur. A biological study area (BSA) was delineated around the PIA, within which most Project-related biological surveys and assessments are being conducted.

The Project site is located within a landscape dominated by gently rolling terrain and grasslands, interspersed with several, mostly ephemeral, riparian corridors and drainages. Numerous wildlife species are known to occur in the region, some of which have been identified as candidate, sensitive, or special-status species in local or regional plans, policies, or regulations, or by the California Department of Fish and Wildlife (CDFW) and/or U.S. Fish and Wildlife Service (USFWS).

In particular, the endangered giant kangaroo rat (Dipodomys ingens) has been reported to occur within the Cholame Valley and the endangered Tipton kangaroo rat (Dipodomys nitratoides nitratoides) is known to occur within the region. In addition, the short-nosed kangaroo rat (D. n. brevinasus), a CDFW Species of Special Concern, has been documented to occur east of the Project site. In addition, Heermann’s kangaroo rat (Dipodomys heermanni) is known from this region as is the potential for the narrow-faced kangaroo rat (Dipodomys venustus sanctiluciae) to occur, neither of which has been identified as a special-status species. Therefore, the purpose of our survey effort was to characterize the occurrence of kangaroo rat species and to confirm the species identification of the kangaroo rats and other small mammals occurring within the Project site and along the access road.

The results reported here along with the results of previous wildlife surveys conducted throughout the Project site and access road provide strong evidence the only species of kangaroo rat present on the site is the Heermann’s kangaroo rat. The small mammal trapping survey resulted in the capture of 36 kangaroo rats. Morphometric measurements taken in the field of 36 kangaroo rats captured during focused trapping efforts indicated that none of the kangaroo rats trapped were Tipton, short-nosed, or giant kangaroo rats. A genetic analysis of the trapped specimens confirmed that all of the trapped individuals were Heermann’s kangaroo rats.

California Flats Solar Project H. T. Harvey & Associates i Small Mammal Trapping Survey October 2013

Table of Contents

Section 1.0 Introduction ...... 4 Section 2.0 Methods ...... 7 2.1 Study Area ...... 7 2.2 Survey Methods ...... 7 2.2.1 Trapping Survey ...... 7 2.2.2 Genetic Analysis ...... 7 Section 3.0 Results ...... 10 3.1 Trapping Survey ...... 10 3.2 Genetic Analysis ...... 11 Section 4.0 Discussion...... 14 4.1 Ecological and Morphological Characteristics ...... 14 4.1.1 Short-nosed and Tipton Kangaroo Rats ...... 14 4.1.2 Heermann’s Kangaroo Rat ...... 14 4.1.3 Narrow-faced Kangaroo Rat ...... 15 4.1.4 Giant Kangaroo Rat ...... 15 4.2 Genetic Analysis ...... 17 Section 5.0 Conclusion ...... 18 5.1 Short-nosed and Tipton Kangaroo Rats ...... 18 5.2 Narrow-faced Kangaroo Rat ...... 18 5.3 Giant Kangaroo Rat ...... 18 5.4 Heermann’s Kangaroo Rat...... 18 Section 6.0 References ...... 19 Appendix A. Datasheets and Photographs of Kangaroo Rats Trapped at the California Flats Solar Project Site, August 2013 ...... 21

Figures and Tables

Figure 1. Occurrences of Special-status Species within a 32 km Radius of the Project Site ...... 6 Figure 2. Trapping Survey Locations within the Project Site and along the Access Road ...... 8 Figure 3. Phylogenetic Tree of Kangaroo Rats Sampled from the Project Site with Reference Species...... 13 Table 1. Small Mammal Trapping Survey Results, California Flats Solar Project, August 2013 ...... 10 Table 2. Position of Distinguishing Base Pairs and the Nucleotide State for Each Species...... 11 Table 3. Morphometrics of Kangaroo Rat Species Potentially Occurring within the Project Area ...... 15 Table 4. Morphometric Characteristics of Giant Kangaroo Rats, Northern Carrizo Plain ...... 16

California Flats Solar Project H. T. Harvey & Associates ii Small Mammal Trapping Survey October 2013

Contributors

Brian Boroski, Ph.D., Vice President and Senior Wildlife Ecologist—Principal-in-Charge Daniel G. Duke, J.D., Associate, Senior Regulatory Specialist Robert K. Burton, Ph.D., Senior Ecologist, Project Manager Howard O. Clark, Jr., M.S., Senior Ecologist Dou-Shaun Yang, Ph.D., Geneticist/Wildlife Ecologist

California Flats Solar Project H. T. Harvey & Associates iii Small Mammal Trapping Survey October 2013

Section 1.0 Introduction

The California Flats Solar Project (Project) is a 280-megawatt photovoltaic solar power plant proposed for development in southeastern Monterey County, California. When approved, the solar facility and related operational infrastructure will be built on approximately 1037 hectares (2562 acres) (Project site) of private ranchland. The 29,137-hectare (72,000-acre) Jack Ranch is a working cattle ranch located in an unincorporated area of southeastern Monterey County and northeastern San Luis Obispo County, near the borders of Kings and Fresno counties. The Project will include construction, installation, and operation of energy-related infrastructure (e.g., solar panels, inverters, substations, and new power poles and transmission lines) and improvements needed to operate and maintain energy-related facilities (e.g., buildings, internal roadways, access roads, fencing, and lighting). The overall development will also include improvements to an existing access road and its connection to State Route (Hwy) 41, approximately 8 kilometers (5 miles) south of the Project site. The access road/Hwy 41 improvement areas will encompass approximately 21.4 hectares (53 acres). Together, the Project site and access road/Hwy 41 improvement areas constitute the 1058-hectare (2615-acre) Project impact area (PIA), where all direct, Project-related impacts will occur. A biological study area (BSA) also was delineated around the PIA, within which most Project-related biological surveys and assessments are being conducted. In addition, the Morrow Bay–Gates 230-kilovolt transmission line crosses the Project site, with capacity sufficient to accommodate the new power plant.

The Project site is located within a landscape dominated by gently rolling terrain and grasslands, interspersed with several, mostly ephemeral, riparian corridors and drainages. Numerous wildlife species are known to occur within the region, some of which have been identified as candidate, sensitive, or special-status species in local or regional plans, policies, or regulations, or by the California Department of Fish and Wildlife (CDFW) and/or U.S. Fish and Wildlife Service (USFWS).

For example, the CDFW California Natural Diversity Database (CNDDB) contains multiple records of the state- and federally listed endangered giant kangaroo rat (Dipodomys ingens) occurring within 32 kilometers (~20 miles) of the Project site, including a single record from the Cholame Valley. The Cholame Valley record, which some experts question, is dated 16 June 1999 and describes 3–4 giant kangaroo rat burrow systems, at a location approximately 0.6 kilometer (0.4 mile) southeast of the Hwy 41 interchange with Hwy 46 (Figure 1). The record includes a reference to the expansion and relocation of the highway interchange, indicating that the observation may have been the result of a biotic study conducted to evaluate impacts of pending highway construction activity.

In addition, there are multiple records of short-nosed kangaroo rat (D. nitratoides brevinasus) and Tipton kangaroo rat (D. n. nitratoides) occurrences within 32 kilometers (~20 miles) of the Project site (Figure 1). The short-nosed kangaroo rat is listed by the state as a California mammalian Species of Special Concern (Brylski 1988), and the Tipton kangaroo rat was listed as endangered by the USFWS in 1988 and by CDFW the following year (CDFW 2013). There are no CNDDB (2013) records of Tipton or short-nosed kangaroo rats

California Flats Solar Project 4 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

occurring within the Project area or the Cholame Valley, and although these areas have not recently been extensively surveyed for these species, the PIA is well outside the known historical range of the species (Grinnell 1922).

In order to characterize the occurrence of kangaroo rat species, along with other wildlife, within the BSA, H. T. Harvey & Associates conducted full-coverage ground surveys to identify and map suitable habitats, identify and map burrows and dens suitable for wildlife and particularly for special-status species, and identify and map other direct or indirect evidence (e.g., scat, tracks) indicating the presence of these species. Full-coverage ground surveys were conducted over 10 days between 6 and 20 November 2012, during which 1123 indirect or direct signs of wildlife species were identified and recorded (H. T. Harvey & Associates 2013a). During these surveys, kangaroo rat burrows, which could be attributed to Heermann’s kangaroo rat (D. heermanni) or possibly to the narrow-faced kangaroo rat (D. venustus sanctilucia) were identified.

To confirm the species identification of the kangaroo rats and other small mammals occurring within the Project site and along the access road, we conducted extensive trapping surveys at locations where burrows were identified during full coverage ground surveys. In addition to field identification of trapped individuals, we conducted genetic analysis of hair samples collected from these individuals and compared the derived genetic sequences with those of known reference specimens of Heermann’s kangaroo rat, narrow-faced kangaroo rat, and giant kangaroo rat.

California Flats Solar Project 5 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

American badger Tipton kangaroo rat San Joaquin kit fox San Joaquin kit fox Coast Range newt San Joaquin kit fox merlin western pond turtle San Joaquin kit fox burrowing owl western pond turtle LEGEND blunt-nosed leopard lizard blunt-nosed leopard lizard pallid bat short-nosed kangaroo rat San Joaquin kit fox tricolored blackbird San Joaquin whipsnake Swainson's hawkburrowing owl short-nosed kangaroo rat San Joaquin kit fox San Joaquin kit fox San Joaquin whipsnake long-eared owl San Joaquin kit fox San JoaquinWildlife kit fox Survey Boundary Hopping's blister beetle San Joaquin pocket mouse San Joaquin kit fox San Joaquin kit fox San Joaquin kit fox blunt-nosed leopard lizard western spadefoot Nelson's antelope squirrel Swainson's hawk redheaded sphecid waspMorrison's blister beetleLe Conte's thrasher Swainson's hawk western mastiff batshort-nosed kangaroo rat silvery legless lizard burrowing owl San Joaquin kit fox San Joaquin kit fox San Joaquin kit fox black-crowned night heron American badgerblunt-nosed leopard lizard Access Road California tiger salamander San Joaquin kit fox burrowing owl Swainson's hawk western pond turtleSan Joaquin whipsnake blunt-nosed leopard lizard loggerhead shrike San Joaquin kit fox western pond turtle Nelson's antelope squirrel blunt-nosed leopard lizard San Joaquin kit foxhoary bat blunt-nosed leopard lizard blunt-nosed leopard lizard 20-Mile Radius foothill yellow-legged frogAmerican badger blunt-nosed leopard lizard San Joaquin kit fox California tiger salamander western pond turtle San Joaquin kit fox blunt-nosed leopard lizard Nelson's antelope squirrel San Joaquin kit fox Nelson's antelope squirrel San Joaquin kit fox San Joaquin dune beetle burrowing owl San Joaquin kit foxAmerican badger blunt-nosed leopard lizard San Joaquin pocket mouse tricolored blackbird San Joaquin kit fox 5-Mile Radius San Joaquin kit fox Nelson's antelope squirrel San Joaquin dune beetleSan Joaquin kit fox San Joaquin kit fox western spadefoot San Joaquin pocket mouse Monterey dusky-footed woodrat western spadefoot burrowing owl San Joaquin kit fox American badger San Joaquin kit fox blunt-nosed leopard lizardTulare grasshopper mouse San Joaquin kit fox burrowing owl blunt-nosed leopard lizardAmerican badgerSan Joaquin kit fox 1-Mile Radius San Joaquin kit fox Nelson's antelope squirrel San Joaquin pocket mouse San Joaquin kit fox San Joaquin kit foxNelson's antelope squirrel San Joaquin dune beetle San Joaquin pocket mouse burrowing owl San Joaquin pocket mouse Tipton kangaroo rat Nelson's antelope squirrel CNDDB Records blunt-nosed leopard lizard San Joaquin dune beetle San Joaquin kit fox San Joaquin kit fox burrowing owl Animals San Joaquin whipsnakeburrowing owl Doyen's trigonoscuta dune weevil San Joaquin kit fox San Joaquin dune beetle burrowing owl burrowing owl Monterey dusky-footedSpecific woodrat Location blunt-nosed leopard lizard San Joaquin kit fox burrowing owl San Joaquin whipsnake San Joaquin pocket mouse western snowy plover Approximate Location blunt-nosed leopard lizard blunt-nosed leopard lizard San Joaquin kit fox

Nelson's antelope squirrel Tipton kangaroo rat San Joaquin kit fox San Joaquin kit fox northern harrier General Area American badger blunt-nosed leopard lizard western snowy plover western pond turtle

San Joaquin kit fox San Joaquin kit foxtricolored blackbird San Joaquin kit fox San Joaquin kit fox tricolored blackbird Tipton kangaroo rat San Joaquin kit foxleast Bell's vireoCalifornia horned lark San Joaquin pocket mouse San Joaquin kit fox 5.5 2.75 0 5.5 short-nosed kangaroo rat white-faced ibis white-faced ibis yellow warblerSan Joaquin kit fox CTS San Joaquin kit fox San Joaquin kit fox San Joaquin kit fox blunt-nosed leopard lizard tricolored blackbirdSan Joaquin kit fox western spadefoot San Joaquin kit foxblunt-nosed leopard lizard Tulare grasshopper mouse Miles San Joaquin whipsnakeCalifornia horned lark San Joaquin kit fox blunt-nosed leopard lizard western snowy plover American badger giant kangaroo rat San Joaquin kit fox Nelson's antelope squirrel San Joaquin kit fox pallid bat San Joaquin kit fox blunt-nosed leopard lizard San Joaquin kit fox San Joaquin pocket mouse Nelson's antelope squirrel blunt-nosed leopard lizard San Joaquin kit foxAmerican badger San Joaquin pocket mouse San Joaquin kit fox burrowing owl tricolored blackbird American badger San Joaquin pocket mouse San Joaquin kit fox fulvous whistling-duck short-nosed kangaroo rat western spadefoot American badger short-nosed kangaroo rat San Joaquin kit foxSan Joaquin kit fox San Joaquin kit fox San Joaquin kit fox San Joaquin pocket mouse western snowy plover San Joaquin whipsnake blunt-nosed leopard lizard burrowing owl blunt-nosed leopard lizard Swainson's hawk San Joaquin whipsnake blunt-nosed leopard lizard San Joaquin kit fox burrowing owl San Joaquin kit fox blunt-nosed leopard lizard bald eagle western spadefoot blunt-nosed leopard lizard burrowing owl western spadefootwestern spadefootwestern pond turtle coast horned lizard San Joaquin kit fox Nelson's antelope squirrel blunt-nosed leopard lizard western pond turtlevernal pool fairy shrimpSan Joaquin kit fox CTS San Joaquin kit fox burrowing owl San Joaquin kit fox fulvous whistling-duck San Joaquin whipsnakewestern spadefoot Nelson's antelope squirrel molestan blister beetle silvery legless lizard mountain plover burrowing owl tricolored blackbird burrowing owl pallid bat CRLF white-faced ibis Salinas pocket mouseAmerican badger western spadefoot burrowing owl bald eagle American badger CRLF CRLF burrowing owl San Joaquin kit fox snowy egret American badgerAmerican badger grasshopper sparrow mountain plover burrowing owl burrowing owl San Joaquin kit fox San Joaquin kit foxblunt-nosed leopard lizard San Joaquin kit fox San Joaquin kit foxcoast horned lizard blunt-nosed leopard lizard black-crowned night heron San Joaquin kit fox San Joaquin pocket mouse Nelson's antelope squirrel Nelson's antelope squirrel giant kangaroo rat San Joaquin pocket mouse San Joaquin kit foxSan Joaquin pocket mouse pallid bat Nelson's antelope squirrel San Joaquin kit fox tricolored blackbird western pond turtle coast horned lizard blunt-nosed leopard lizard Nelson's antelope squirrelSan Joaquin kit fox San Joaquin kit fox San Joaquin kit fox American badger Monterey dusky-footed woodrat pallid bat burrowing owlSan Joaquin kit fox merlin blunt-nosed leopard lizard Tipton kangaroo rat silvery legless lizard merlin burrowing owl Salinas pocket mouse western pond turtle CRLF San Joaquin kit fox blunt-nosed leopard lizard bald eagle pallid bat San Joaquin kit fox silvery legless lizardwestern pond turtle western spadefoot Tulare grasshopper mouse blunt-nosed leopard lizard San Joaquin kit fox western snowy ploverblunt-nosed leopard lizard San Joaquin kit fox Monterey dusky-footed woodrat San Joaquin kit foxSan Joaquin kit foxburrowing owl San Joaquin kit fox CTS San Joaquin kit fox least Bell's vireo Nelson's antelope squirrel American badger western spadefootsilvery legless lizard blunt-nosed leopard lizard blunt-nosed leopard lizardburrowing owl American badger western pond turtle CRLF San Joaquin pocket mouse westernCTS spadefoot Nelson's antelope squirrelSan Joaquin kit fox San Joaquin kit fox western pond turtle vernal pool fairy shrimp San Joaquin kit fox American badgerSan Joaquin whipsnake San Joaquin kit fox bank swallow blunt-nosed leopard lizardSan Joaquin kit fox Nelson's antelope squirrel vernal pool fairy shrimp San Joaquin kit fox western pond turtle Tulare grasshopper mouse CTS San Joaquin kit foxSan Joaquin kit fox blunt-nosed leopard lizard Atascadero June beetle San Joaquin kit fox burrowing owl San Joaquin kit fox burrowing owl blunt-nosed leopard lizard burrowing owl western pond turtle Lompoc grasshopperSan Joaquin kit fox San Joaquin kit fox burrowing owlblunt-nosed leopard lizard San Joaquin kit fox burrowing owlSan Joaquin kit fox western pond turtleSan Joaquin kit fox Nelson's antelope squirrel blunt-nosed leopard lizard Tipton kangaroo rat Nelson's antelope squirrel western pond turtle western spadefoot San Joaquin kit fox San Joaquin kit fox San Joaquin kit foxTipton kangaroo rat blunt-nosed leopard lizard burrowing owlSan Joaquin kit foxNelson's antelope squirrel San Joaquin kit fox western spadefoot Tulare grasshopper mouse CTS American badgerSan Joaquin pocket mouse CTSSan Joaquin kit fox Nelson's antelope squirrelSan Joaquin kit fox San Joaquin kit fox burrowing owl Nelson's antelope squirrel two-striped garter snake giant kangaroo rat burrowing owl CRLF blunt-nosed leopard lizard blunt-nosed leopard lizardSan Joaquin pocket mouseSan Joaquin kit fox steelhead - south/central California coast DPS burrowing owl American badgerAmerican badger burrowing owl San Joaquin kit fox burrowing owl Coast Range newt American badger burrowing owl San Joaquin kit fox Nelson's antelope squirrel short-nosed kangaroo rat American badgergiant kangaroo rat San Joaquin kit fox San Joaquin kit fox western pond turtle San Joaquin kit fox San Joaquin kit fox blunt-nosed leopard lizard monarch butterfly western spadefoot American badger burrowing owl western spadefoot western spadefoot San Joaquin kit fox grasshopper sparrow western pond turtle CTS San Joaquin kit fox burrowing owl San Joaquin kit fox blunt-nosed leopard lizard Tipton kangaroo rat California red-legged frog California red-legged frog San Joaquin kit fox American badger San Joaquin kit foxblunt-nosed leopard lizard western pond turtle California red-legged frog vernal pool fairy shrimp blunt-nosed leopard lizard California red-legged frog San Joaquin kit fox San Joaquin kit fox San Joaquin kit fox San Joaquin kit fox western spadefoot San Joaquin kit fox San Joaquin kit fox western pond turtle Atascadero June beetle western pond turtle giant kangaroo rat giant kangaroo rat San Joaquin kit fox giant kangaroo rat western pond turtle silvery legless lizard giant kangaroo rat Nelson's antelope squirrel western pond turtle giant kangaroo rat Tipton kangaroo rat California red-legged frogwestern pond turtle Nelson's antelope squirrel giant kangaroo rat American badger Nelson's antelope squirrel monarch butterfly Atascadero June beetle western spadefoot San Joaquin pocket mouse western pond turtle blunt-nosed leopard lizard San Joaquin kit fox western pond turtle western pond turtle American badger Coast Range newt western spadefoot western spadefoot western spadefoot American badger Nelson's antelope squirrel San Joaquin kit fox Nelson's antelope squirrel tidewater goby western pond turtle San Joaquin kit fox San Joaquin kit fox California red-legged frogtidewater goby western pond turtle purple martin western spadefoot San Joaquin kit fox San Joaquin kit fox San Joaquin kit foxSan Joaquin kit fox monarch butterflywestern pond turtle western spadefoot pallid bat Le Conte's thrasher western mastiff bat sandy beach tiger beetlesteelhead - south/central California coast DPS Sources: Esri, DeLorme, NAVTEQ, USGS, Intermap, iPC, NRCAN, Esri Japan, METI, Esri China (Hong Kong), Esri (Thailand), TomTom, 2013

J:\Reports\Wildlife Reports\Burrowing Mammals and Bird Surveys\Oct 2013\Fig 1SS specieswithin 32 km radius.mxd western spadefoot San Joaquin kit fox Nelson's antelope squirrel Figure 1: Occurrences of Special-status Species within a 32 kilometer Radius of the Project Site California Flats Solar Project, Burrowing Mammals and Bird Surveys (3308-01) October 2013

Section 2.0 Methods

2.1 Study Area

The small mammal trapping survey was focused on the Project site and access corridor. Traps were set at 35 locations (Figure 2), where kangaroo rat burrows were previously identified during full-coverage ground surveys of the Project site and access road.

2.2 Survey Methods

2.2.1 Trapping Survey

For each night of trapping, H. B. Sherman XLK folding traps were distributed and set at sunset. Traps were set in clusters in areas containing high concentrations of kangaroo rat burrows showing sign of recent activity. A total of 200 traps were deployed each night, placed in six clusters of 30 traps and one cluster of 20 traps. Traps were baited with white millet and provisioned with unbleached paper towels. Traps were checked at least every three hours, and trapping was ceased immediately in the event of inclement weather such as rain, high winds, or low temperature. Each morning all traps were recovered, closed, and secured in plastic snap- top containers.

For each captured animal, a series of metrics were recorded, including sex, age, weight, hind foot length, and general health. Hair samples were collected from the animal’s dorsum, for genetic analysis, and stored in 2-milliliter vials containing 1 milliliter of RNALater™ solution, using a new vial for each sample. Each trapped animal was marked with a nontoxic marker to allow for identification if recaptured, then released at the site of capture.

2.2.2 Genetic Analysis

Genomic DNA was extracted from hair samples collected from each of the kangaroo rats trapped on the Project site and access road. In addition, we extracted DNA from two tissue samples of narrow-faced kangaroo rat (UC Berkeley Museum of Vertebrate Zoology—Mammal Collection numbers 198290 and 181290) and two samples of giant kangaroo rat (California Polytechnic State University San Luis Obispo— Frozen Collection numbers 223 and 224) for use as species identification reference samples.

Genomic DNA was extracted from hair samples with a DNEasy Blood & Tissue DNA extraction kit™ (Qiagen, Inc., Valencia, California) following the provided procedure. The DNA extractions were performed in batches of 16 hair samples alongside an additional negative control sample to control for possible DNA contamination. Sampling instruments were sterilized by being dipped in ethanol and flamed between each sample.

California Flats Solar Project 7 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

TURKEY FLAT RD k

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Transmission Line Corridor

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Biological Study Area

Existing Transmission Lines

Group of Traps 20 Traps

STATE HIGHWAY 41 30 Traps

3,500 1,750 0 3,500

Feet J:\Misc\Wildlife\Fig 2 Trapping Survey Locations within the Project Siteand along theAccess Road. .mxd Figure 2: Trapping Survey Locations within the Project Site andSTATE along HIGHWAY the 46 Access Road California Flats Solar Project (3308-01) October 2013

Following DNA extraction, we amplified a fragment of the mitochondrial DNA (mtDNA) control region for each sample using polymerase chain reaction (PCR; Mullis and Faloona 1987). PCR amplifications were carried out in a 25-microliter (µl) volume with 14.9-µl PCR-grade water (Sigma-Aldrich.com), 1 µl per 25-

millimeter (mm) MgCl2, 2.5 µl 10X JumpStart REDTaq™ buffer (Sigma-Aldrich.com), 0.474 µl 10-mm dNTP mix (Perkin-Elmer.com); 2.5 µl 1-mm THR light-strand primer (5’- TCAAAGCTTACACCAGTCTTGTAAACC-3’; Kocher et al. 1989), 2.5 µl 1-mm TDKD heavy-strand primer (5’-CCTGAAGTAGGAACCAGATF-3’; Kocher et al. 1993), 0.125 µl JumpStart REDTaq™, and 1 µl of extracted DNA template.

Cycling conditions consisted of an initial denaturation of 94°C for 5 minutes; followed by 45 cycles of 94°C for 30 seconds, 48°C for 30 seconds, and 72°C for 30 seconds; followed by a final extension of 72°C for 10 minutes. Each batch of PCR amplifications also contained a negative amplification control using the negative extraction control as a template, as well as a positive control using a DNA template extracted from giant kangaroo rat liver tissue. All PCR amplification products were evaluated for successful amplification using agarose gel electrophoresis. The negative amplification controls did not successfully amplify in any of the PCR batches.

Following successful amplification of DNA from the 36 hair samples collected from kangaroo rats trapped at the Project site and the reference specimens, we submitted the samples to Sequetech, Inc. (Mountain View, California), which provides a complete DNA Sequencing Service. At Sequetech, the PCR products were cleaned using EXO-SAPit™ (Affymetrix Inc., Santa Clara, California), then each sample was Sanger- sequenced in the forward and reverse direction using the provided PCR primers.

After acquiring mtDNA control-region sequence data for the 36 kangaroo rats from the Project site and the references samples, we imported these data, along with D. venustus, D. ingens, and two D. heermanni reference sequences (Genbank Accessions AF225931 and AF225949) into the analytical software program 4Peaks (Nucleobytes.com) for sequence editing. We then imported the edited sequences into the bioinformatics software program Genious R6™ (Biomatters, Inc., San Francisco, California) to concatenate the forward and reverse sequence data for each sample and generate a consensus sequence. We aligned these consensus sequences using the default alignment settings, and analyzed this alignment for nucleotide polymorphisms that could be used to distinguish the mtDNA of narrow-faced kangaroo rat, giant kangaroo rat, and Heermann’s kangaroo rat.

To determine the species membership of each sample, we inferred a tree of phylogenetic relationships between the samples collected from trapped kangaroo rats and each species’ reference samples, using a neighbor-joining algorithm with a Jukes-Cantor model of DNA substitution and 100 bootstrap- pseudoreplications to estimate nodal support. The tree was rooted with a D. microps reference sequence (Genbank Accession AF136529).

California Flats Solar Project 9 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

Section 3.0 Results

3.1 Trapping Survey

A total of 1400 traps were set and monitored over 7 nights during the period of 5 August through 15 August 2013. A total of 42 kangaroo rats were trapped, including five recaptures (Table 1). All of the individuals captured were determined to be adults based on pelage, body size, and weight, and were identified in the field to be either Heermann’s kangaroo rat or possibly narrow-faced kangaroo rat.

Table 1. Small Mammal Trapping Survey Results, California Flats Solar Project, August 2013

Specimen ID Sex Weight Hind Foot Ear Comments Age (grams) (mm) (mm) 0805A F Adult 59.0 38.5 14.0 0805B F Adult 95.0 45.0 17.0 0805C F Adult 68.0 40.0 13.0 0805D F Adult 58.0 40.5 14.0 0805E F Adult 60.0 40.0 14.0 0805F F Adult 65.0 42.0 14.0 0805G M Adult 79.0 42.0 17.0 0805H - - - - - Recapture, not reprocessed 0805I M Adult 79.0 40.0 15.0 0805J F Adult 66.0 42.0 13.0 0806A F Adult 77.0 41.5 16.0 0806B M Adult 74.0 40.5 15.0 0806C M Adult 78.0 42.5 14.0 0806D M Adult 74.0 41.3 15.0 0806E - - - - - Recapture, not reprocessed 0806F F Adult 67.0 - - Escaped, partially processed 0806G - - - - - Recapture, not reprocessed 0806H M Adult 80.0 42.5 17.0 0806I F Adult 68.0 39.0 14.0 0806J M Adult 70.0 42.0 14.5 0807A M Adult 76.0 43.0 16.0 0807B M Adult 78.0 39.5 15.0 0807C F Adult 66.0 41.9 15.0 0807D F Adult 72.0 42.4 16.0 0807E F Adult 63.0 41.9 16.0 0807F F Adult 80.0 42.5 17.0 0807G M Adult 70.0 40.1 15.0 0807H F Adult 75.0 41.0 16.0 0807I F Adult 58.0 42.0 15.0 0807J F Adult 57.0 40.0 16.0 0808A - - - - - Recapture, not reprocessed 0808B F Adult 70.0 39.5 17.0

California Flats Solar Project 10 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

Table 1 (cont.). Small Mammal Trapping Survey Results, California Flats Solar Project, August 2013

Specimen ID Sex Age Weight Hind Foot Ear Comments (g) (mm) (mm) 0808C F Adult 86.0 43.0 18.0 0808D F Adult 67.0 39.5 14.0 0808E M Adult 89.0 42.1 15.0 0808F M Adult 72.0 43.5 16.0 0808G F Adult 73.0 41.5 15.0 0808H M Adult 71.0 40.0 16.0 0808I F Adult 70.0 41.0 16.0 0812A F Adult 65.0 39.8 16.0 0812B M Adult 75.0 41.8 15.0 0813A - - - - - Recapture, not reprocessed

3.2 Genetic Analysis

We acquired a total of 474 base pairs of data for each sample. Of these 474 base pairs, 32 base pairs could be used to distinguish D. heermanni individuals from D. ingens, 27 to distinguish D. heermanni from D. venustus, and 28 to distinguish D. ingens from D. venustus. Table 2 summarizes the position of these distinguishing base pairs and the nucleotide state (A, C, T, or G) for each species at that base pair.

Table 2. Position of Distinguishing Base Pairs and the Nucleotide State for Each Species

Base D. heermanni D. ingens D. venustus Base D. heermanni D. ingens D. venustus 33 A T T 262 A A T 38 C G T 263 T A T 57 T T G 264 T T A 82 A A G 274 T G T 86 C C T 275 A G G 132 T A C 276 A A G 177 T T A 278 G G A 178 T G T 280 T G G 209 G A A 285 G T G 245 T A T 292 G T G 223 G T T 293 T A T 229 T A T 294 A G A 231 G A G 297 T A G 232 T G G 298 T G T 234 A C A 302 G A G 235 C T T 304 - A A 240 T T A 306 T A T 241 C T T 308 T A A 255 G T T 315 G T T 257 G A G 334 C T T 258 A A T 335 T A A

California Flats Solar Project 11 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

The phylogenetic tree produced by this analysis shows distinct genetic divisions between Heermann’s kangaroo rat, narrow-faced kangaroo rat, and giant kangaroo rat, with 100% nodal support between species (Figure 3). All of the kangaroo rats from the Project site clustered with the Heermann’s kangaroo rat reference samples, confirming the species identification of D. heermanni for all the kangaroo rats trapped and sampled at the Project site and along the access road.

California Flats Solar Project 12 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

J:\Reports\Wildlife Reports\GeneticTesting\Fig 3 PhylogeneticTree of Kangaroo Rats Sampled from the ProjectSite with Reference Species.mxd Figure 3: Phylogenetic Tree of Kangaroo Rats Sampled from the Project Site with Reference Species California Flats Solar Project (3308-01) October 2013

Section 4.0 Discussion

Five distinct types of kangaroo rat are known to occur within the greater region (approximately 50- kilometer/30-mile radius) surrounding the Project site: Herrmann’s kangaroo rat, giant kangaroo rat, narrow- faced kangaroo rat, short-nosed kangaroo rat, and Tipton kangaroo rat. Any of these species/subspecies could potentially occur within the BSA as the Cholame Valley largely comprises suitable habitat for all five species.

The absence of the short-nosed and Tipton kangaroo rats among the kangaroo rats trapped was readily confirmed through morphometric evaluation in the field. Due to morphometric overlaps between adult Heermann’s and narrow-faced kangaroo rats, and to a lesser degree with subadult giant kangaroo rats, species identification of the kangaroo rats trapped on the Project site and access road was subsequently based on morphometric, ecological, and genetic analysis.

4.1 Ecological and Morphological Characteristics

4.1.1 Short-nosed and Tipton Kangaroo Rats

Both the short-nosed and Tipton kangaroo rat have four toes on the hind foot, whereas giant, narrow-faced, and Heermann’s kangaroo rats each possess five toes (Jameson and Peters 1988). All the kangaroo rats trapped had five toes on the hind foot, confirming that none of the individuals trapped on the Project site or along the access road was a short-nosed or Tipton kangaroo rat.

4.1.2 Heermann’s Kangaroo Rat

The kangaroo rat burrows identified throughout the Project site and along the access road during full- coverage ground surveys had characteristics typical of Heermann’s kangaroo rat burrows, with relatively small openings to horizontal and vertical burrow shafts, limited cropping of vegetation, and scat typical of this species. The Heermann’s kangaroo rat classification comprises at least eight distinct subspecies, widely distributed throughout the central portion of California. The species’ range extends from Suisun Bay south to Point Conception, bordered by the Pacific Ocean and the foothills of the Sierra Nevada and the Tehachapi Mountains. The Project site is well within the range of the Heermann’s kangaroo rat, and the open grasslands on the Project site and along the access road are typical habitat for this species (Kelt 1988).

The characteristics of the kangaroo rats trapped at the Project site and along the access road were typical of Heermann’s kangaroo rats. However, some of the trapped individuals possessed characteristics that indicated they could be narrow-faced kangaroo rats.

California Flats Solar Project 14 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

4.1.3 Narrow-faced Kangaroo Rat

The narrow-faced kangaroo rat has a fairly restricted range that includes the Santa Lucia Mountains, extending from Monterey Bay to San Luis Obispo, bordered by the Salinas Valley to the east and the Pacific Ocean to the west (Grinnell 1922). The Project site is outside the known range of the narrow-faced kangaroo rat, but the species is recorded to have occurred in locations to the south and west of the site, and there is potentially suitable habitat connecting these locations to the Cholame Valley.

Although narrow-faced kangaroo rats are, on average, heavier than Heermann’s kangaroo rats, there is a considerable range of weights for both species and therefore an overlap between the species. The pelage of the narrow-faced kangaroo rat is reportedly darker than that of the Heermann’s kangaroo rat (Best 1992); however, there is likely to be substantial variation of this characteristic between populations of both species, rendering identification based on this trait less objective. Nonetheless, several kangaroo rats captured during the trapping survey did have markedly darker pelage characteristics than what is typical for Heermann’s kangaroo rat.

The most distinctive morphometric characteristic of the narrrow-faced kangaroo rat is ear length, which for 65 male and 73 female specimens averaged 18.6 mm and 18.2 mm, respectively (Best 1993). By comparison, ear length measurements averaged 15.0 mm and 14.6 mm for 366 male and 474 female Heermann’s kangaroo rats respectively (Best 1993); however, the subspecies or locales of the latter specimens were not specified (Table 3).

Table 3. Morphometrics of Kangaroo Rat Species Potentially Occurring within the Project Area

Ear Hind Foot Species Sex Weight (g) (mm) (mm) References

M 122.01 (n = 90) 17.02 (n = 13) 48.51 (n = 89) H. T. Harvey & Associates 2013b D. ingens F 120.01 (n = 80) 16.72 (n = 5) 47.91 (n = 80) Williams and Kilburn 1991 M 82.03 (n = 2) 18.64 (n = 65) 45.84 (n = 65) Grinnell 1922, 1919 D. venustus F 87.73 (n = 1) 18.24 (n = 73) 45.14 (n = 73) 4Best 1993 M 60.85 (n = 44) 15.04 (n = 474) 43.34 (n = 474) Potter et al. 2010 D. heermanni F 55.85 (n = 46) 14.64 (n = 366) 42.64 (n = 366) Best 1993

Although the Project site is well outside the known geographic range of the narrow-faced kangaroo rat, five kangaroo rats captured at the Project site had ear lengths of 17.0 mm, and one individual had an ear length of 18.0 mm; these measurements indicated that the individuals could be narrow-faced kangaroo rats.

4.1.4 Giant Kangaroo Rat

No burrows attributable to the giant kangaroo rat have been identified during full-coverage ground surveys or incidentally during other wildlife surveys of the Project site and access road. The burrows of giant kangaroo rats are particularly distinctive, with large, very well-maintained horizontal burrow openings, nearly perfectly

California Flats Solar Project 15 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

round vertical shaft openings, and extensive cropping of vegetation around the burrow precinct, which is typically accompanied by extensive “haystacking” of cut vegetation and numerous pit-caches where seeds are dried (Williams and Kilburn 1991). Due to the extent of activity on the surface at a giant kangaroo rat burrow precinct, particularly with regard to cropping of vegetation, the burrows can be readily detected at a distance, and can even be identified during aerial surveys and from satellite imagery (Bean et al. 2012).

Large body size is the most distinctive morphological characteristic separating giant kangaroo rats from other species occurring in the region. The average weights of male and female giant kangaroo rats are nearly double the average body mass of male and female Heermann’s kangaroo rats and more than 30 grams heavier than the type specimens of narrow-faced kangaroo rat (Grinnell 1919).

All of the individuals trapped at the Project site were identified as adults, which is to be expected given the timing of the trapping survey relative to the known breeding season of the giant kangaroo rat (January–May; Williams and Kilburn 1991), narrow-faced kangaroo rat (January–May; Best 1992), and Heermann’s kangaroo rat (January–June; Kelt 1988). The largest kangaroo rats trapped at the Project site were two females that weighed 95 grams and 86 grams, along with a male that weighed 89 grams. The remaining kangaroo rats trapped at the Project site had body weights of 80 grams or less. In contrast, male giant kangaroo rats from the northern Carrizo Plain weighed, on average, 122 grams, with a range of 100–150 grams, and non- reproductive females weighed, on average, 120 grams, with a range of 92–138 grams, with gravid females reaching weights in excess of 150 grams (Table 4; H. T. Harvey & Associates 2013b).

Table 4. Morphometric Characteristics of Giant Kangaroo Rats, Northern Carrizo Plain

Mean Hind Foot Length Mean Weight Gender (millimeter) (grams) Adults

Males 48.5 (n = 89; SD 1.7) 122 (n = 90; SD 13.0) Females 47.9 (n = 80; SD 2.0) 120 (n = 80; SD 13.5) Juveniles

Males 47.5 (n = 12; SD 1.7) 92 (n = 12; SD 17.5) Females 46.9 (n = 37; SD 1.9) 82 (n = 38; SD 20.3)

The hind foot length of kangaroo rats can also be used to distinguish these species. The largest individual captured at the Project site was the female described above, with a weight of 95 grams. The hind foot of this individual was 45.0 mm long (Table 1; Specimen 0805b). The average hind foot length of female giant kangaroo rats from the northern Carrizo Plain is 47.9 mm, and for males, the average length is 48.5 mm (H. T. Harvey & Associates 2013b).

California Flats Solar Project 16 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

4.2 Genetic Analysis

The results of the genetic polymorphism and phylogenetic inference analyses clearly segregate the kangaroo rats trapped at the Project site and along the access road from narrow-faced rat and giant kangaroo rat reference samples; the results confirm that the kangaroo rats occurring at the Project site and along the access road are Heermann’s kangaroo rats.

California Flats Solar Project 17 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

Section 5.0 Conclusion

5.1 Short-nosed and Tipton Kangaroo Rats

The short-nosed and Tipton kangaroo rat are not known to have occurred, recently or historically, within the Cholame Valley and no evidence indicating the presence of these subspecies’ of kangaroo rat has been identified during any of the wildlife surveys of the Project site including the small-mammal trapping survey. Subsequently the short-nosed kangaroo rat and Tipton kangaroo rat are considered absent from the BSA.

5.2 Narrow-faced Kangaroo Rat

The possible presence of the narrow-faced kangaroo rat was evaluated through both morphometric and genetic analyses. Although some of the characteristics of kangaroo rats trapped at the Project site and access road indicated a possible species identity of narrow-faced kangaroo rat, the genetic analysis clearly demonstrates genetic alignment with known specimens of Heermann’s kangaroo rat and separation from known samples of narrow-faced kangaroo rat. Based on the results of the full-coverage ground surveys and the genetic analysis of samples gathered during the trapping survey, and on the fact that this species is not known to have occurred recently or historically within the Cholame Valley (Grinnell 1922), we consider the narrow-faced kangaroo rat to be absent from the BSA.

5.3 Giant Kangaroo Rat

There is a record from 1999 of the giant kangaroo rat occurring in the Cholame Valley (CNDDB 2013), although historical and recent range maps do not show the species’ range extending into the Cholame Valley (Grinnell 1922; Williams 1992). No evidence indicating the presence of giant kangaroo rat burrow precincts has been identified during the full-coverage ground surveys of the Project site and access road, or during other wildlife surveys, including the trapping survey. Based on the results of the full-coverage ground surveys and the genetic analysis of trapped kangaroo rats, we consider the giant kangaroo rat to be absent from the BSA.

5.4 Heermann’s Kangaroo Rat

Burrows identified as typical of Heermann’s kangaroo rat were identified at numerous locations throughout the Project site and along the access road. Extensive trapping surveys of these areas resulted in capture of 36 unique individual kangaroo rats, all of which have been confirmed through genetic analysis to be Heermann’s kangaroo rats. No other species or subspecies of kangaroo rat has been identified or observed on the Project site or along the access road.

California Flats Solar Project 18 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

Section 6.0 References

Bean W. T., R. Stafford, L. R. Prugh, H. S. Butterfield, J. S. Brashares. 2012. An evaluation of monitoring methods for the endangered giant kangaroo rat. Wildlife Society Bulletin 36(3):587-593.

Best T. L. 1992. Dipodomys venustus. Mammalian Species 403:1-4.

Best, T. L. 1993. Patterns of morphologic and morphometric variation in heteromyid rodents. Pages 197-235 in H. H. Genoways, and J. H. Brown (Editors), Biology of the Heteromyidae. Special Publication No. 10. The American Society of Mammalogists.

Brylski, P. V. 1988. Short-nosed kangaroo rat Dipodomys nitratoides brevinasus. Pages 97-100 in B. C. Bolster (Editor), Terrestrial Mammal Species of Special Concern in California.

[CDFW] 2013. California Department of Fish and Wildlife. State and Federally Listed Endangered and Threatened Animals of California. Online October 8 2013:

http://www.dfg.ca.gov/biogeodata/cnddb/pdfs/TEAnimals.pdf

[CNDDB] California Natural Diversity Database. 2013. Rarefind 3.1.0, a program created by the California Department of Fish and Game, allowing access to the CNDDB.

Grinnell, J. 1919. Four new kangaroo rats from west-central California. Proceedings of the Biological Society of Washington 32:203-206.

Grinnell, J. 1922. A geographical study of the kangaroo rats of California. University of California Publications in Zoology 24:1-124.H. T. Harvey & Associates. 2013a. California Flats Solar Project, Monterey County, California, Biotic Report. July. Prepared for California Flats, LLC, Minneapolis, Minnesota. Fresno, California.

H. T. Harvey & Associates. 2013b. California Valley Solar Ranch Project Giant Kangaroo Rat Relocation Weekly Report 27 July – 2 August 2013.

Jameson, E. W., Jr., and H. J. Peeters. 1988. California Mammals. University of California Press, Berkeley, CA.

Kelt, D. A. 1988. Dipodomys heermanni. Mammalian Species 323:1-7.

Kocher, T. D., J. A. Conroy, K. R. McKaye, and J. R. Stauffer 1993. Similar morphologies of cichlid fish in Lakes Tanganyika and Malawi are due to convergence. Molecular Phylogenetics and Evolution 2:158- 165.

California Flats Solar Project 19 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

Mullis, K. B., and F. A. Faloona. 1987. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods in Enzymology 155:335–350.

Potter, M. C., H. O. Clark Jr., P. A. Kelly, and C. E. Uptain. 2010. Fire effects on a population of Heermann’s kangaroo rats at the Alkali Sink Ecological Reserve, Fresno County, California. Transactions of the Western Section of the Wildlife Society 46:1–6.

Williams, D. F. 1992. Geographic distribution and population status of the giant kangaroo rat, Dipodomys ingens (Rodentia, Heteromyidae). Pages 301–328 in D. F. Williams, S. Byrne, and T. A. Rado (Editors), Endangered and Sensitive Species of the San Joaquin Valley, California: Their Biology, Management, and Conservation. The California Energy Commission, Sacramento, California.

Williams, D. F., and K. S. Kilburn. 1991. Dipodomys ingens. Mammalian Species 377:1–7.

[USFWS] U.S. Fish and Wildlife Service. 1988. Endangered and Threatened Wildlife and Plants; Determination of Endangered Status for the Tipton Kangaroo Rat. Federal Register 53:25608– 25611.

California Flats Solar Project 20 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

Appendix A. Datasheets and Photographs of Kangaroo Rats Trapped at the California Flats Solar Project Site, August 2013

California Flats Solar Project 21 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 1 ID 0805A

Date: 05 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0743868 3971421

Release Location (GPS Coordinates): 10S 0743868 3971421

Time of Capture and/or Observation: 2327

Time of Release: 2345

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 59

Hind foot measurement (mm): 38.5

Ear length (mm): 14

Ambient Temperature When Handled and Released (ºF): 66.0

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Not in reproductive condition.

DNA Sample Taken Photos Taken

Other Notes: Skies: clear.

Initials of Staff Completing This Form: CAW

California Flats Solar Project 22 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 23 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 2 ID 0805B

Date: 05 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0744621 3970296

Release Location (GPS Coordinates): 10S 0744621 3970296

Time of Capture and/or Observation: 0003

Time of Release: 0020

Species: Dipodomys venustus Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 95

Hind foot measurement (mm): 45

Ear length (mm): 17

Ambient Temperature When Handled and Released (ºF): 66.0

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. In reproductive condition, possibly pregnant.

DNA Sample Taken Photos Taken

Other Notes: Dark mask and pelage. Skies: clear.

Initials of Staff Completing This Form: CAW

California Flats Solar Project 24 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 25 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 3 ID 0805C

Date: 06 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0743786 3971549

Release Location (GPS Coordinates): 10S 0743786 3971549

Time of Capture and/or Observation: 0622

Time of Release: 0637

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 68

Hind foot measurement (mm): 40

Ear length (mm): 13

Ambient Temperature When Handled and Released (ºF): 53.2

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. In reproductive condition.

DNA Sample Taken Photos Taken

Other Notes: Skies: clear.

Initials of Staff Completing This Form: CAW

California Flats Solar Project 26 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 27 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 4 ID 0805D

Date: 06 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0743877 3971435

Release Location (GPS Coordinates): 10S 0743877 3971435

Time of Capture and/or Observation: 0653

Time of Release: 0701

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 58

Hind foot measurement (mm): 40.5

Ear length (mm): 14

Ambient Temperature When Handled and Released (ºF): 53.2

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, agitated. No injuries or parasites. Not in reproductive condition.

DNA Sample Taken Photos Taken

Other Notes: Skies: clear.

Initials of Staff Completing This Form: CAW

California Flats Solar Project 28 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 29 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 5 ID 0805E

Date: 06 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0744638 3970460

Release Location (GPS Coordinates): 10S 0744638 3970460

Time of Capture and/or Observation: 0710

Time of Release: 0720

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 60

Hind foot measurement (mm): 40

Ear length (mm): 14

Ambient Temperature When Handled and Released (ºF): 62

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, agitated and struggling. No injuries or parasites. Not in reproductive condition.

DNA Sample Taken Photos Taken

Other Notes: No photos were taken due to the animal being under stress; was overly agitated and struggling too much to photograph; animal released without photos. Skies: clear.

Initials of Staff Completing This Form: CAW

NO PHOTOS—STRESSED INDIVIDUAL

California Flats Solar Project 30 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 6 ID 0805F

Date: 06 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0744638 3970406

Release Location (GPS Coordinates): 10S 0744638 3970406

Time of Capture and/or Observation: 0730

Time of Release: 0745

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 65

Hind foot measurement (mm): 42

Ear length (mm): 14

Ambient Temperature When Handled and Released (ºF): 62

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Not in reproductive condition.

DNA Sample Taken Photos Taken

Other Notes: Skies: clear.

Initials of Staff Completing This Form: CAW

California Flats Solar Project 31 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 32 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 7 ID 0805G

Date: 06 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0744629 3970317

Release Location (GPS Coordinates): 10S 0744629 3970317

Time of Capture and/or Observation: 0745

Time of Release: 0800

Species: Dipodomys venustus Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 79

Hind foot measurement (mm): 42

Ear length (mm): 17

Ambient Temperature When Handled and Released (ºF): 68

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. In reproductive condition.

DNA Sample Taken Photos Taken

Other Notes: Dark pelage. Skies: clear.

Initials of Staff Completing This Form: CAW

California Flats Solar Project 33 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 34 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 35 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 8 ID 0805H

Date: 06 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0744629 3970307

Release Location (GPS Coordinates): 10S 0744629 3970307

Time of Capture and/or Observation: 0800

Time of Release: 0805

Species: Dipodomys venustus—RECAP Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 95

Hind foot measurement (mm): 45

Ear length (mm): 17

Ambient Temperature When Handled and Released (ºF): 68

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

RECAPTURE. Appeared in good health, alert, calm. No injuries or parasites. In reproductive condition.

DNA Sample Taken Photos Taken

Other Notes: RECAPTURE—did not process, released when observed marked UTM approximate several meters south of previous capture. Dark pelage. Skies: clear.

Initials of Staff Completing This Form: CAW

NO EXTRA PHOTOS—RECAPTURE

California Flats Solar Project 36 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 9 ID 0805I

Date: 06 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0744579 3970231

Release Location (GPS Coordinates): 10S 0744579 3970231

Time of Capture and/or Observation: 0805

Time of Release: 0820

Species: Dipodomys venustus Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 79

Hind foot measurement (mm): 40

Ear length (mm): 15

Ambient Temperature When Handled and Released (ºF): 68

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. In reproductive condition.

DNA Sample Taken Photos Taken

Other Notes: Dark mask and pelage. Skies: clear.

Initials of Staff Completing This Form: CAW

California Flats Solar Project 37 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 38 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 10 ID 0805J

Date: 06 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0744553 3970215

Release Location (GPS Coordinates): 10S 0744553 3970215

Time of Capture and/or Observation: 0820

Time of Release: 0830

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 66

Hind foot measurement (mm): 42

Ear length (mm): 13

Ambient Temperature When Handled and Released (ºF): 68

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Not in reproductive condition.

DNA Sample Taken Photos Taken

Other Notes: Pale pelage, mask less pronounced/contrasting. Skies: clear.

Initials of Staff Completing This Form: CAW

California Flats Solar Project 39 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 40 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 11 ID 0806A

Date: 06 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0743939 3971406

Release Location (GPS Coordinates): 10S 0743939 3971406

Time of Capture and/or Observation: 2229

Time of Release: 2239

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 77

Hind foot measurement (mm): 41.5

Ear length (mm): 16

Ambient Temperature When Handled and Released (ºF): 60.0

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Not in reproductive condition.

DNA Sample Taken Photos Taken

Other Notes: Skies: clear.

Initials of Staff Completing This Form: HOC

California Flats Solar Project 41 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 42 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 12 ID 0806B

Date: 06 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0743905 3971347

Release Location (GPS Coordinates): 10S 0743905 3971347

Time of Capture and/or Observation: 2243

Time of Release: 2253

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 74

Hind foot measurement (mm): 40.5

Ear length (mm): 15

Ambient Temperature When Handled and Released (ºF): 60.0

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Reproductive condition: SCRO.

DNA Sample Taken Photos Taken

Other Notes: Skies: clear.

Initials of Staff Completing This Form: HOC

California Flats Solar Project 43 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 44 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 13 ID 0806C

Date: 06 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0744258 3969532

Release Location (GPS Coordinates): 10S 0744258 3969532

Time of Capture and/or Observation: 2305

Time of Release: 2315

Species: Dipodomys venustus Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 78

Hind foot measurement (mm): 42.5

Ear length (mm): 14

Ambient Temperature When Handled and Released (ºF): 59.0

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Reproductive condition: SCRO.

DNA Sample Taken Photos Taken

Other Notes: Skies: clear.

Initials of Staff Completing This Form: HOC

California Flats Solar Project 45 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 46 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 14 ID 0806D

Date: 07 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0743953 3971397

Release Location (GPS Coordinates): 10S 0743953 3971397

Time of Capture and/or Observation: 0631

Time of Release: 0638

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 74

Hind foot measurement (mm): 41.25

Ear length (mm): 15

Ambient Temperature When Handled and Released (ºF): 51.2

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Reproductive condition: SCRO.

DNA Sample Taken Photos Taken

Other Notes: Skies: marine layer.

Initials of Staff Completing This Form: HOC

California Flats Solar Project 47 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 48 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 15 ID 0806E

Date: 07 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0743943 3971399

Release Location (GPS Coordinates): 10S 0743943 3971399

Time of Capture and/or Observation: 0639

Time of Release: 0640

Species: Dipodomys heermanni—RECAP Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): — Hind foot measurement (mm): —

Ear length (mm): —

Ambient Temperature When Handled and Released (ºF): 52

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

RECAP.

DNA Sample Taken Photos Taken

Other Notes: Skies: marine layer.

Initials of Staff Completing This Form: HOC

NO PHOTOS—RECAPTURE

California Flats Solar Project 49 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 16 ID 0806F

Date: 07 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0744261 3969551

Release Location (GPS Coordinates): 10S 0744261 3969551

Time of Capture and/or Observation: 0709

Time of Release: 0715

Species: Dipodomys heermanni—ESCAPED Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 67

Hind foot measurement (mm): —

Ear length (mm): —

Ambient Temperature When Handled and Released (ºF): 52

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Reproductive condition: post lactating.

DNA Sample Taken Photos Taken

Other Notes: Skies: marine layer.

Initials of Staff Completing This Form: HOC

NO PHOTOS—ESCAPED

California Flats Solar Project 50 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 17 ID 0806G

Date: 07 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0744274 3969549

Release Location (GPS Coordinates): 10S 0744274 3969549

Time of Capture and/or Observation: 0719

Time of Release: 0720

Species: Dipodomys venustus—RECAP Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 76

Hind foot measurement (mm): —

Ear length (mm): —

Ambient Temperature When Handled and Released (ºF): 54

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

RECAP.

DNA Sample Taken Photos Taken

Other Notes: Skies: marine layer. SCRO.

Initials of Staff Completing This Form: HOC

NO PHOTOS—RECAPTURE

California Flats Solar Project 51 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 18 ID 0806H

Date: 07 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0744195 3969441

Release Location (GPS Coordinates): 10S 0744195 3969441

Time of Capture and/or Observation: 0733

Time of Release: 0743

Species: Dipodomys venustus Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 80

Hind foot measurement (mm): 42.5

Ear length (mm): 17

Ambient Temperature When Handled and Released (ºF): 55

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Reproductive condition: SCRO.

DNA Sample Taken Photos Taken

Other Notes: Skies: marine layer.

Initials of Staff Completing This Form: HOC

California Flats Solar Project 52 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 53 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 19 ID 0806I

Date: 07 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0744138 3969266

Release Location (GPS Coordinates): 10S 0744138 3969266

Time of Capture and/or Observation: 0752

Time of Release: 0759

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 68

Hind foot measurement (mm): 39

Ear length (mm): 14

Ambient Temperature When Handled and Released (ºF): 57

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Reproductive condition: Lactating.

DNA Sample Taken Photos Taken

Other Notes: Skies: marine layer.

Initials of Staff Completing This Form: HOC

California Flats Solar Project 54 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 55 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 20 ID 0806J

Date: 07 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0744302 3969082

Release Location (GPS Coordinates): 10S 0744302 3969082

Time of Capture and/or Observation: 0822

Time of Release: 0832

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 70

Hind foot measurement (mm): 42

Ear length (mm): 14.5

Ambient Temperature When Handled and Released (ºF): 59

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Reproductive condition: SCRO.

DNA Sample Taken Photos Taken

Other Notes: Skies: clear.

Initials of Staff Completing This Form: HOC

California Flats Solar Project 56 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 57 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 21 ID 0807A

Date: 07 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0741622 3973020

Release Location (GPS Coordinates): 10S 0741622 3973020

Time of Capture and/or Observation: 2256

Time of Release: 2308

Species: Dipodomys venustus Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 76

Hind foot measurement (mm): 43

Ear length (mm): 16

Tail length (cm): 18

Body length (cm): 12.5

Ambient Temperature When Handled and Released (ºF): 62.4

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Ears were tattered from previous injuries. In reproductive condition.

DNA Sample Taken Photos Taken

Other Notes: Dark pelage and mask. Skies: clear.

Initials of Staff Completing This Form: CAW

California Flats Solar Project 58 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 59 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 22 ID 0807B

Date: 08 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0741248 3973201

Release Location (GPS Coordinates): 10S 0741248 3973201

Time of Capture and/or Observation: 0636

Time of Release: 0645

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 78

Hind foot measurement (mm): 39.5

Ear length (mm): 15

Tail length (cm): 17.5

Body length (cm): 13

Ambient Temperature When Handled and Released (ºF): 52.5

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Ears were tattered from previous injuries. In reproductive condition.

DNA Sample Taken Photos Taken

Other Notes: Light pelage and faint mask. Skies: clear.

Initials of Staff Completing This Form: CAW

California Flats Solar Project 60 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 61 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 23 ID 0807C

Date: 08 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0741630 3972980

Release Location (GPS Coordinates): 10S 0741630 3972980

Time of Capture and/or Observation: 0652

Time of Release: 0700

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 66

Hind foot measurement (mm): 41.9

Ear length (mm): 15

Tail length (cm): 17

Body length (cm): 10.5

Ambient Temperature When Handled and Released (ºF): 54

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Appeared to be post lactating.

DNA Sample Taken Photos Taken

Other Notes: Light pelage and faint mask. Skies: clear.

Initials of Staff Completing This Form: CAW

California Flats Solar Project 62 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 63 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 24 ID 0807D

Date: 08 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0741613 3973024

Release Location (GPS Coordinates): 10S 0741613 3973024

Time of Capture and/or Observation: 0702

Time of Release: 0712

Species: Dipodomys venustus Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 72

Hind foot measurement (mm): 42.4

Ear length (mm): 16

Tail length (cm): 15

Body length (cm): 10.5

Ambient Temperature When Handled and Released (ºF): 54

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert. No injuries or parasites. Appeared to be post lactating.

DNA Sample Taken Photos Taken

Other Notes: Dark pelage and mask. Skies: clear.

Initials of Staff Completing This Form: CAW

California Flats Solar Project 64 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 65 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 25 ID 0807E

Date: 08 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0742112 3972704

Release Location (GPS Coordinates): 10S 0742112 3972704

Time of Capture and/or Observation: 0734

Time of Release: 0743

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 63

Hind foot measurement (mm): 41.9

Ear length (mm): 16

Tail length (cm): 14.5

Body length (cm): 12

Ambient Temperature When Handled and Released (ºF): 59

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Not in reproductive condition.

DNA Sample Taken Photos Taken

Other Notes: Pelage and mask not distinctively dark or light. Skies: clear.

Initials of Staff Completing This Form: CAW

California Flats Solar Project 66 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 67 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 26 ID 0807F

Date: 08 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0742121 3972709

Release Location (GPS Coordinates): 10S 0742121 3972709

Time of Capture and/or Observation: 0749

Time of Release: 0755

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 80

Hind foot measurement (mm): 42.5

Ear length (mm): 17

Tail length (cm): 17.5

Body length (cm): 12

Ambient Temperature When Handled and Released (ºF): 59

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No parasites. Appeared pregnant. Abrasion on right heel.

DNA Sample Taken Photos Taken

Other Notes: Pelage and mask not distinctively dark or light. Skies: clear.

Initials of Staff Completing This Form: CAW

California Flats Solar Project 68 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 69 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 27 ID 0807G

Date: 08 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0742135 3972713

Release Location (GPS Coordinates): 10S 0742135 3972713

Time of Capture and/or Observation: 0758

Time of Release: 0803

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 70

Hind foot measurement (mm): 40.1

Ear length (mm): 15

Tail length (cm): 17

Body length (cm): 12

Ambient Temperature When Handled and Released (ºF): 61

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. In reproductive condition.

DNA Sample Taken Photos Taken

Other Notes: Pelage and mask not distinctively dark or light. Skies: clear.

Initials of Staff Completing This Form: CAW

California Flats Solar Project 70 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 71 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 28 ID 0807H

Date: 08 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0742540 3972461

Release Location (GPS Coordinates): 10S 0742540 3972461

Time of Capture and/or Observation: 0811

Time of Release: 0820

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 75

Hind foot measurement (mm): 41

Ear length (mm): 16

Tail length (cm): 16.5

Body length (cm): 11

Ambient Temperature When Handled and Released (ºF): 61

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Right ear appeared red in color. Appeared to be post lactating.

DNA Sample Taken Photos Taken

Other Notes: Pelage light brown and faint mask. Skies: clear.

Initials of Staff Completing This Form: CAW

California Flats Solar Project 72 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 73 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 29 ID 0807I

Date: 08 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0742916 3972247

Release Location (GPS Coordinates): 10S 0742916 3972247

Time of Capture and/or Observation: 0830

Time of Release: 0838

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 58

Hind foot measurement (mm): 42

Ear length (mm): 15

Tail length (cm): 16

Body length (cm): 11

Ambient Temperature When Handled and Released (ºF): 62

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Not in reproductive condition.

DNA Sample Taken Photos Taken

Other Notes: Pelage brown and faint mask. Skies: clear.

Initials of Staff Completing This Form: CAW

California Flats Solar Project 74 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 75 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 30 ID 0807J

Date: 08 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0742912 3972232

Release Location (GPS Coordinates): 10S 0742912 3972232

Time of Capture and/or Observation: 0840

Time of Release: 0848

Species: Dipodomys venustus Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 57

Hind foot measurement (mm): 40

Ear length (mm): 16

Tail length (cm): 15

Body length (cm): 11

Ambient Temperature When Handled and Released (ºF): 62

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in fair health, alert, stressed. Nose heavily abraded; blood on animal and in trap appears to originate in upper corner of door. Not in reproductive condition.

DNA Sample Taken Photos Taken

Other Notes: Pelage moderately dark and dark complete mask. Skies: clear.

Initials of Staff Completing This Form: CAW

California Flats Solar Project 76 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 77 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 31 ID 0808A

Date: 08 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0741605 3973036

Release Location (GPS Coordinates): 10S 0741605 3973036

Time of Capture and/or Observation: 2239

Time of Release: 2245

Species: Dipodomys heermanni—RECAP Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 72

Hind foot measurement (mm): __

Ear length (mm): __

Tail length (cm): 17

Body length (cm): 11.1

Ambient Temperature When Handled and Released (ºF): 62

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Post lactating.

DNA Sample Taken Photos Taken

Other Notes: Pelage brown and faint mask. Skies: clear.

Initials of Staff Completing This Form: HOC

NO PHOTOS—RECAPTURE

California Flats Solar Project 78 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 32 ID 0808B

Date: 09 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0740600 3973678

Release Location (GPS Coordinates): 10S 0740600 3973678

Time of Capture and/or Observation: 0616

Time of Release: 0626

Species: Dipodomys venustus Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 70

Hind foot measurement (mm): 39.5

Ear length (mm): 17

Tail length (cm): 15

Body length (cm): 12.5

Ambient Temperature When Handled and Released (ºF): 50.5

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Reproductive condition: post reproductive.

DNA Sample Taken Photos Taken

Other Notes: Skies: marine layer.

Initials of Staff Completing This Form: HOC

California Flats Solar Project 79 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 80 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 33 ID 0808C

Date: 09 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0741225 3973199

Release Location (GPS Coordinates): 10S 0741225 3973199

Time of Capture and/or Observation: 0639

Time of Release: 0649

Species: Dipodomys venustus Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 86

Hind foot measurement (mm): 43

Ear length (mm): 18

Tail length (cm): 18

Body length (cm): 13

Ambient Temperature When Handled and Released (ºF): 50.5

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Reproductive condition: lactating, pregnant.

DNA Sample Taken Photos Taken

Other Notes: Skies: marine layer.

Initials of Staff Completing This Form: HOC

California Flats Solar Project 81 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 82 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 34 ID 0808D

Date: 09 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0742142 3972682

Release Location (GPS Coordinates): 10S 0742142 3972682

Time of Capture and/or Observation: 0719

Time of Release: 0725

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 67

Hind foot measurement (mm): 39.5

Ear length (mm): 14

Tail length (cm): 15.5

Body length (cm): 12.5

Ambient Temperature When Handled and Released (ºF): 53.8

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Reproductive condition: pregnant.

DNA Sample Taken Photos Taken

Other Notes: Skies: marine layer.

Initials of Staff Completing This Form: HOC

California Flats Solar Project 83 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 84 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 35 ID 0808E

Date: 09 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0742142 3972682

Release Location (GPS Coordinates): 10S 0742142 3972682

Time of Capture and/or Observation: 0726

Time of Release: 0736

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 89

Hind foot measurement (mm): 42.1

Ear length (mm): 15

Tail length (cm): 17.6

Body length (cm): 13

Ambient Temperature When Handled and Released (ºF): 53.8

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Reproductive condition: SCRO.

DNA Sample Taken Photos Taken

Other Notes: Skies: marine layer.

Initials of Staff Completing This Form: HOC

California Flats Solar Project 85 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 86 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 36 ID 0808F

Date: 09 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0742170 3972704

Release Location (GPS Coordinates): 10S 0742170 3972704

Time of Capture and/or Observation: 0739

Time of Release: 0746

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 72

Hind foot measurement (mm): 43.5

Ear length (mm): 16

Tail length (cm): 18.5

Body length (cm): 13.5

Ambient Temperature When Handled and Released (ºF): 53.8

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Reproductive condition: SCRO.

DNA Sample Taken Photos Taken

Other Notes: Skies: marine layer.

Initials of Staff Completing This Form: HOC

California Flats Solar Project 87 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 88 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 37 ID 0808G

Date: 09 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0742170 3972703

Release Location (GPS Coordinates): 10S 0742170 3972703

Time of Capture and/or Observation: 0747

Time of Release: 0751

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 73

Hind foot measurement (mm): 41.5

Ear length (mm): 15

Tail length (cm): 17.2

Body length (cm): 12.3

Ambient Temperature When Handled and Released (ºF): 53.8

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Reproductive condition: pregnant.

DNA Sample Taken Photos Taken

Other Notes: Skies: marine layer.

Initials of Staff Completing This Form: HOC

California Flats Solar Project 89 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 90 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 38 ID 0808H

Date: 09 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0742176 3972707

Release Location (GPS Coordinates): 10S 0742176 3972707

Time of Capture and/or Observation: 0752

Time of Release: 0759

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 71

Hind foot measurement (mm): 40

Ear length (mm): 16

Tail length (cm): 17.3

Body length (cm): 11.4

Ambient Temperature When Handled and Released (ºF): 55.1

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Reproductive condition: SCRO.

DNA Sample Taken Photos Taken

Other Notes: Skies: clear.

Initials of Staff Completing This Form: HOC

California Flats Solar Project 91 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 92 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 39 ID 0808I

Date: 09 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0742916 3972275

Release Location (GPS Coordinates): 10S 0742916 3972275

Time of Capture and/or Observation: 0825

Time of Release: 0835

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 70

Hind foot measurement (mm): 41

Ear length (mm): 16

Tail length (cm): 17.2

Body length (cm): 13

Ambient Temperature When Handled and Released (ºF): 60.5

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Appeared in good health, alert, calm. No injuries or parasites. Not in reproductive condition.

DNA Sample Taken Photos Taken

Other Notes: Skies: clear.

Initials of Staff Completing This Form: HOC

California Flats Solar Project 93 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 94 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 40 ID 0812A

Date: 13 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0744811 3964564

Release Location (GPS Coordinates): 10S 0744811 3964564

Time of Capture and/or Observation: 0626

Time of Release: 0636

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 65

Hind foot measurement (mm): 39.8

Ear (mm): 16

Body length (cm): 12

Tail length (cm): 16.5

Ambient Temperature When Handled and Released (ºF): 56.5

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Healthy, no parasites. Minor nose abrasion. Not in reproductive condition.

DNA Sample Taken Photos Taken

Other Notes: Pale pelage and mask.

Initials of Staff Completing This Form: CAW

California Flats Solar Project 95 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 96 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 41 ID 0812B

Date: 13 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0744792 3964396

Release Location (GPS Coordinates): 10S 0744792 3964396

Time of Capture and/or Observation: 0709

Time of Release: 0717

Species: Dipodomys heermanni Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 75

Hind foot measurement (mm): 41.8

Ear (mm): 15

Body length (cm): 12

Tail length (cm): 16.5

Ambient Temperature When Handled and Released (ºF): 62

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Healthy, no parasites. Minor nose abrasion. In reproductive condition.

DNA Sample Taken Photos Taken

Other Notes: Pale pelage and mask.

Initials of Staff Completing This Form: CAW

California Flats Solar Project 97 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project 98 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013

California Flats Solar Project Trapping Data Sheet

Number 42 ID 0813A

Date: 13 August 2013

Personnel Involved: Howard Clark, Colin Wilkinson

Permit Numbers:

Capture Location (GPS Coordinates): 10S 0744813 3964566

Release Location (GPS Coordinates): 10S 0744813 3964566

Time of Capture and/or Observation: 2253

Time of Release: 2259

Species: Dipodomys heermanni—RECAP Sex: Male Female

Age: Juvenile Sub-Adult Adult

Weight (g): 66

Hind foot measurement (mm): 41.2

Ear (mm): 15

Body length (cm): 12.4

Tail length (cm): Not taken

Ambient Temperature When Handled and Released (ºF): 75.6

General condition and health (note all visible conditions including gait and behavior, diarrhea, emaciation, salivation, hair loss, ectoparasites, injuries, etc.):

Healthy, no parasites. Minor nose abrasion. Not in reproductive condition.

DNA Sample Taken Photos Taken

Other Notes: Pale pelage and mask.

Initials of Staff Completing This Form: HOC

NO PHOTOS—RECAPTURE

California Flats Solar Project 99 H. T. Harvey & Associates Small Mammal Trapping Survey October 2013