PIAS1, Protein inhibitor of activated STAT 1 [C-terminus] polyclonal antibody

rotein inhibitor of activated STAT 1 (PIAS1) is a member or Research Use Only. Not for Pof the mammalian PIAS family, proteins which contains FDiagnostic or Therapeutic Use. a SAP domain, a putative DNA-binding (bihelical) motif Purchase does not include or carry predicted to be involved in chromosomal organisation. It any right to resell or transfer this inhibits STAT1-mediated activation and its DNA binding product either as a stand-alone product or as a component of another activity, binds to Gu protein/RNA helicase II/DEAD box product. Any use of this product other polypeptide 21, and interacts with androgen receptor (AR). than the permitted use without the It functions in testis as a nuclear receptor transcriptional express written authorization of Allele coregulator and may have a role in AR initiation and Biotech is strictly prohibited maintenance of spermatogenesis. Buffers Purified chicken polyclonal antibody supplied in PBS with Website: www.allelebiotech.com 0.09% (W/V) sodium azide. This antibody is purified through Call: 1-800-991-RNAi/858-587-6645 a protein G column and eluted out with both high and low (Pacific Time: 9:00AM~5:00PM) pH buffers and neutralized immediately after elution then Email: [email protected] followed by dialysis against PBS. Immunogen KLH conjugated synthetic peptide comprised of amino Box 1 | Basic Info acids 621 - 649 [RESHSHTVTNRSSTDT (621-636 aa)] of

the human protein inhibitor of activated STAT 1 (PIAS1) Cat. No. ABP-PAB-10342 [C-terminus] protein. Animal ID RB0669-0670 Application: Host Rabbit Tested by peptide-specific ELISA (1:1,000). WB (1:100 Reactivity Human ~1:500), IHC (1:50 ~1:100) Format Purified Accession number NM_016166 Storage Amount 100µg Maintain refrigerated at 2-8ºC for up to 6 months. For long term storage store at -20ºC. Avoid repeated freeze-thaw Alternative Name(s): protein inhibitor of cycles. activated STAT, 1, GBP, DDXBP1, GU/RH-II, Gu binding protein References:

1. Tan JA, Hall SH, Hamil KG, Grossman G, Petrusz P, French FS: Protein inhibitors of activated STAT resemble scaffold attachment factors and function as interacting nuclear receptor coregulators. J. Biol. Chem. 277: 16993- 17001 (2002). 2. Megidish T, Xu JH, Xu CW: Activation of by protein inhibitor of activated Stat1 (PIAS1). J. Biol. Chem. 277: 8255-8259 (2002). 3. Liu B, Shuai K: Induction of apoptosis by protein inhibitor of activated Stat1 through c-Jun NH2-terminal kinase activation. J. Biol. Chem. 276: 36624-36631 (2001). 4. Osaka F, Kawasaki H, Aida N, Saeki M, Chiba T, Kawashima S, Tanaka K, Kato S: A new NEDD8-ligating system for cullin-4A. Dev. 12: 2263-2268 (1998). 5. Amir RE, Iwai K, Ciechanover A: The NEDD8 pathway is essential for SCF(beta -TrCP)-mediated ubiquitination and processing of the NF-kappa B precursor p105. J. Biol. Chem. 277: 23253-23259 (2002).

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