Anti-Ranbp9 / RANBPM Antibody (ARG54076)

Product datasheet [email protected]

ARG54076 Package: 100 μl anti-RanBP9 / RANBPM antibody Store at: -20°C

Summary

Product Description Mouse Monoclonal antibody recognizes RanBP9 / RANBPM

Tested Reactivity Hu

Tested Application ICC/IF, IP, WB

Host Mouse

Clonality Monoclonal

Isotype IgG1

Target Name RanBP9 / RANBPM

Species Human

Immunogen Purified recombinant human RanBP9 protein fragments expressed in E.coli.

Conjugation Un-conjugated

Alternate Names RANBPM; RanBPM; BPM-L; Ran-binding protein 9; RanBP9; BPM90; RanBP7; Ran-binding protein M

Application Instructions

Application table Application Dilution

ICC/IF 1:300

IP Assay-dependent

WB 1:1000

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Observed Size 78 kDa

Properties

Form Liquid

Purification Affinity purified

Buffer 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol

Preservative 0.2% Sodium azide

Stabilizer 50% Glycerol

Concentration 1 mg/ml

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

www.arigobio.com 1/3 Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links GeneID: 10048 Human

Swiss-port # Q96S59 Human

Gene Symbol RANBP9

Gene Full Name RAN binding protein 9

Background May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. May be involved in signaling of ITGB2/LFA-1 and other integrins. Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but not affect estrogen- induced transactivation. Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity. Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA.

Function May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. May be involved in signaling of ITGB2/LFA-1 and other integrins. Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but not affect estrogen- induced transactivation. Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity. Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA (By similarity). [UniProt]

Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody

Calculated Mw 78 kDa

PTM Phosphorylated in response to stress. Can be phosphorylated by the cleaved p110 form of CDC2L1 (p110C). Ubiquitinated. Polyubiquitination targets the protein for rapid degradation via the ubiquitin system. Can be deubiquitinated by USP11.

Cellular Localization Cytoplasm, cytosol. Nucleus.

Images

ARG54076 anti-RanBP9 / RANBPM antibody ICC/IF image

Immunofluorescence: HeLa cells stained with ARG54076 anti- RanBP9 / RANBPM antibody at 1:300 dilution.

www.arigobio.com 2/3 ARG54076 anti-RanBP9 / RANBPM antibody WB image

Western blot: CEM cell lysate stained with ARG54076 anti-RanBP9 / RANBPM antibody at 1:1000 dilution.

ARG54076 anti-RanBP9 / RANBPM antibody IP image

Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG54076 anti-RanBP9 / RANBPM antibody.