plants Article Integrative Taxonomic, Ecological and Genotyping Study of Charophyte Populations from the Egyptian Western-Desert Oases and Sinai Peninsula Abdullah A. Saber 1 , Andrey A. Gontcharov 2, Arthur Yu. Nikulin 2 , Vyacheslav Yu. Nikulin 2 , Walaa A. Rayan 1 and Marco Cantonati 3,* 1 Botany Department, Faculty of Science, Ain Shams University, Abbassia Square, Cairo 11566, Egypt;
[email protected] (A.A.S.);
[email protected] (W.A.R.) 2 Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, 159, 100-Letia Vladivostoka Prospect, 690022 Vladivostok, Russia;
[email protected] (A.A.G.);
[email protected] (A.Y.N.);
[email protected] (V.Y.N.) 3 MUSE—Museo delle Scienze, Limnology & Phycology Section, Corso del Lavoro e della Scienza 3, I-38123 Trento, Italy * Correspondence:
[email protected]; Tel.: +39-0461-270342 Abstract: Present-day information available on the charophyte macroalgae in Egypt, including their phylogenetic affinities, remains largely incomplete. In this study, nine charophyte populations were collected from different aquatic biotopes across the Egyptian Western-Desert Oases and Sinai Citation: Saber, A.A.; Gontcharov, Peninsula. All populations were investigated using an integrative polyphasic approach including A.A.; Nikulin, A.Y.; Nikulin, V.Y.; phylogenetic analyses inferred from the chloroplast-encoded gene (rbcL) and the internal transcribed Rayan, W.A.; Cantonati, M. spacer (ITS1) regions, in parallel with morphotaxonomic assignment, ultrastructure of the oospore Integrative Taxonomic, Ecological walls, and autecology. The specimens identified belonged to the genera Chara, Nitella, and Tolypella, and Genotyping Study of Charophyte with predominance of the first genus to which five species were assigned though they presented some Populations from the Egyptian interesting aberrant taxonomic features: C.