Polyclonal Antibody to SMARCE1-Related Protein - Aff - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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AP06758PU-N Polyclonal Antibody to SMARCE1-related protein - Aff - Purified

Alternate names: BRAF35, BRCA2-associated factor 35, HMG box-containing protein 20B, HMG domain- containing protein 2, HMG domain-containing protein HMGX2, HMG20B, HMGX2, HMGXB2, SMARCE1R, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related, Sox-like transcriptional factor, Structural DNA-binding protein BRAF35 Quantity: 0.1 mg Concentration: 1.0 mg/ml Background: The breast cancer susceptibility gene (BRCA1) localizes to chromosome 17q. Mutations within this gene account for approximately 45% of families with high incidence of breast cancer and at least 80% of families with increased incidence of both early-onset breast cancer and ovarian cancer. A second breast cancer susceptibility gene, BRCA2, located on chromosome 13q12-13, also confers a high incidence of breast cancer, but unlike BRCA1, BRCA2 does not confer a substantially elevated risk of ovarian cancer. The BRCA2-Associated Factor 35 (BRAF35) protein forms a 20 kDa complex with BRCA2, which associates with condensed chromatin during histone H3 phosphorylation. BRAF35 expression levels are highest in proliferating tissues and parallel BRCA2 expression patterns. The structure of BRAF35 includes a kinesinlike coiled coil domain and a nonspecific DNA binding HMG domain. The chromatin localization of BRAF35 and antibody microinjection studies indicate a role for the BRAF35/BRCA2 complex in cell cycle regulation. Uniprot ID: Q9P0W2 NCBI: NP_006330.2 GeneID: 10362 Host: Rabbit Immunogen: Synthetic peptide, corresponding to the N-terminual of Human BRAF35. Format: State: Liquid purified Ig fraction Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) Buffer System: Phosphate buffered saline (PBS), pH 7.2. Preservatives: 0.05% sodium azide Applications: Western blot: 1/500-1/1000. Immunohistochemistry on paraffin sections: 1/50-1/200. Immunofluorescence: 1/50-1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Molecular Weight: ~ 36 kDa Specificity: This antibody detects endogenous levels of BRAF35 protein. (region surrounding Thr29)

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. VA/20140923 1 / 2 AP06758PU-N: Polyclonal Antibody to SMARCE1-related protein - Aff - Purified

Species Reactivity: Tested: Human. Expected from sequence similarity: Mouse and Rat. Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Pictures: Western blot (WB) analysis of BRAF35 antibody (Cat.-No.: AP06758PU-N) at 1/500 dilution Lane1:A549 whole cell lysate Lane2:Mouse liver tissue lysate Lane3:Rat liver tissue lysate

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. VA/20140923 2 / 2