Human B Cells from Whole Blood and Bone Marrow

Mouse B cells from bone marrow, spleen, and lymphoid tissue

Bone marrow

Pre-selection with: Pre-enrichment with: B cell Isolation Kit Pre-enrichment with: CD138 MicroBeads Pan B cell Pan B Cell Isolation Kit II Pan B Cell Isolation Kit II Pan B Cell Isolation Kit II Isolation Kit II + CD138 + Plasma Cell CD43 (Ly-48) MicroBeads Isolation Kit Pro B cells Pre B cells Resting B cells Plasmablasts Plasma cells Abnormal B cells

CD19+ CD19+ CD19+ CD19+ CD138 + CD19+ CD43+ CD43low CD20+ CD138 + CD38low CD20+ CD20+/− CD20+ CD43− CD45R(B220)low CD19− CD5+ CD24+ CD24+ CD45R (B220)+ MHC class II+ CD45R(B220)low Ig+/− CD45R(B220)+ CD25+/− Ig+/− IgM/A/E/G+ CD93+/− IgM− CD45R(B220)+ MHC class II+/− IgM− Ig− Body cavities

Spleen/lymphoid tissue

B Cell Isolation MZ and FO B Regulatory B Cell Germinal Center Pre-enrichment CD138 Memory B Cell Pan B cell B-1a Cell B -1a Cell Kit Cell Isolation Kit Isolation Kit B Cell (PNA) with: MicroBeads Isolation Kit Isolation Kit II Isolation Kit Isolation Kit MicroBead Kit Pan B Cell CD23 Isolation Kit II CD138 + Plasma Anti-Mouse IgG1 CD19 MicroBeads CD5 (Ly-1) flow through MicroBeads Cell Isolation Kit MicroBeads MicroBeads of second CD22 MicroBeads magnetic Anti-Mouse selection IgG2a+b CD23 MicroBeads (CD5− cells) MicroBeads CD45R (B220) Anti-Mouse IgM MicroBeads MicroBeads

Resting MZ and FO Regulatory Germinal Plasmablasts Plasma cells Memory Pan B cells B1a cells B1b cells B cells B cells B cells center cells B cells

CD19+ CD19+ CD19 + PNA+ CD19 + CD138 + CD19+ CD19+ CD19+ CD19 + CD20+ CD21+/− CD5+ CD19+ CD138 + CD38low CD20+ CD20+ CD45R(B220)+ CD45R(B220)+ CD43− CD22+ CD24+ CD38low CD45R(B220)low CD19 − CD22+ CD22+ CD5+ CD5− CD45R (B220)+ CD23+/− CD1dhigh CD20+ MHC class II+ CD45R(B220)low CD38+/− CD23+/− CD11b + CD11b + Ig+/− CD93− CD93+/− CD93− IgM/A/E/G+ CD93+/− CD45R(B220)+ CD45R(B220)+ CD43+ CD43+ CD35+/− IL10, GL7+ MHC class II+/− CD95low CD45+ CD23− CD23− CD45R(B220)+ TGFß secretion CD37+ Ig− CD80+/− Ig+/− IgMhigh IgMhigh IgM+/− IgMhigh IgM/A/E/G+ IgM/A/E/G+ IgDlow IgDlow IgD+/− IgD+/− IgD+/− IgD+/−

Legend

separation MZ: marginal zone MHC: major histocompatibility complex – no expression high high expression MACS® Cell Separation products (RUO) FO: follicular Ig: immunoglobulin low low expression +/– expression dependent MACS Flow Cytometry and TGF: transforming growth factor PNA: peanut agglutinin int intermediate expression on subset Cytokine detection products (RUO) CD: cluster of di erentiation + expression

This publication is provided for information purposes only, and the contents hereof are subject to change without notice. While Miltenyi Biotec has taken great care and attention to ensure the quality of the information contained herein at the time of publication, no warranties are provided that the content of this publication is accurate, complete, reliable, up to date, and error-free. Miltenyi Biotec assumes no responsibility or liability arising out of the application or use of any information described herein except as expressly confirmed in writing by authorized representative of Miltenyi Biotec. Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for

therapeutic or diagnostic use. MACS and the Miltenyi Biotec logo are registered trademarks or trademarks of Miltenyi Biotec and/or its affiliates in various countries worldwide. Copyright © 2021 Miltenyi Biotec and/or its affiliates. All rights reserved. 130-124-683.02 Human B cells from whole blood and bone marrow

Whole blood B u ﬀ y c o a t Leukopak LRSC Density gradient

PBMCs

StraightFrom® Whole Blood StraightFrom Buffy Coat StraightFrom Leukopak StraightFrom LRSC CD19 CD19 MicroBead Kit CD19 MicroBead Kit CD19 MicroBead Kit MicroBead Kit StraightFrom Whole Blood MACSxpress Buffy Coat Pan MACSxpress LRSC Pan CD45 MicroBead Kit B Cell Isolation Kit B Cell Isolation Kit

MACSxpress® Whole Blood CD45+ CD3+/− Pan B Cell Isolation Kit CD14+/− CD19+/− CD15+/− CD56+/− MACSxpress Whole Blood + B Cell Isolation Kit HLA-DR

MACSxpress StraightFrom MACSxpress Pan B Cell Isolation Kit Naive B Cell Isolation Kit II B Cell Isolation Kit II CD138 MicroBeads Memory B Cell Anti-ROR1 Whole Blood Whole Blood Whole Blood Isolation Kit MicroBead Kit Naive B Cell CD19 MicroBeads Anti-IgD MicroBeads Plasma Cell Isolation Kit II CD138 B-CLL Cell + Isolation Kit MicroBeads Isolation Kit IgM Memory B Cell CD138 CD19 MultiSort Kit Isolation Kit MicroBeads

StraightFrom CD20 MicroBeads + Whole Blood IgG Memory B Cell B-CLL Cell Anti-ROR1 CD22 MicroBeads Isolation Kit Isolation Kit MicroBead Kit REAlease® CD19 Switched Memory B Cell Pre-enrichment: MicroBead Kit Isolation Kit B Cell Isolation Kit II CD27 MicroBeads + Anti-IgG MicroBeads CD10 MicroBead Kit Anti-IgM MicroBeads Naive B cells Plasma cells Abnormal B cells Pan B cells Naive B cells Resting B cells Plasma cells Memory B cells Abnormal B cells

CD19+ CD19low CD19+ CD19+ CD19+ CD19+ CD19 low CD19+ CD19+ CD20+ CD20− CD20+ CD20+ CD20+ CD20+ CD20− CD20+ CD20+ CD27− CD138+ ROR1+ CD22+ CD27− CD43- CD138+ CD27+/− ROR1+ CD22+ CD38high CD5+ Ig+/− CD22+ CD80/86- CD38high CD38− CD5+ IgD+ CD27+ Ig+/− IgD+ Ig+/- CD27+ CD40+ CD10+ MHC class II+/− MHCI class II+/− CD23low Ig+/− Ig− Ig IgM/A/E/G+ IgD+/− Bone marrow

StraightFrom Whole Blood and Bone Marrow CD138 MicroBeads

Pre-selection with: Pre-selection with: CD138 MicroBeads Anti-ROR1 MicroBead Kit Plasma cells B Cell Isolation Kit II B Cell Isolation Kit II or or Plasma Cell Isolation Kit II CD138 MicroBeads REAlease CD19 REAlease CD19 B-CLL Cell Isolation Kit MicroBead Kit MicroBead Kit + + CD10 and CD34 CD10 MicroBead Kit MicroBead Kits Pro B cells Pre and/or immature B cells Plasma cells Abnormal CLL B cells CD19 low CD27+ CD20 − MHC class II+/− CD138+ Ig− CD38high

CD10+ CD34+ CD10+ CD21+ CD19 low CD27+ CD19 + CD19+ CD24+ CD19+ CD24+ CD20− MHCI class II+/− CD20+ CD38+ IgM− CD20+ IgM+/− CD138+ Ig ROR1+ CD38+ IgD− CD38high CD5+ Ig+/− Legend

direct cell separation products differentiation/cultivation B cell cultivation and activation separation

MACS® Cell Separation products (RUO) MACS Flow Cytometry and Cytokine B cells and B cell subsets Expanded and activated B cells MACS Cell Culture and Stimulation products detection products (RUO)

CLL: chronic lymphocytic leukemia – no expression B Cell Expansion Kit, ROR: receptor tyrosine kinase-like orphan receptor 1 + expression TLR Ligands LRSC: leukocyte reduction system chamber low low expression PBMC: peripheral blood mononuclear cells int intermediate expression Cytokines e.g., IL-4*, IL-21, TNF-α* HLA: human leukocyte antigen high high expression Human CD40-Ligand Multimer Kits TNF: tumor necrosis factor +/– expression dependent on subset CD19 + CD22+ CD19 + CD80/86+ CD25+ CD: cluster of differentiation CD20+ Ig+/− CD20+ CD69+ MHC class IIhigh MHC: major histocompatibility complex *Available also as MACS GMP product CD22+ CD30+ Ig+/− Ig: immunoglobulin

therapeutic or diagnostic use. MACS, MACSxpress, REAlease, StraightFrom and the Miltenyi Biotec logo are registered trademarks or trademarks of Miltenyi Biotec and/or its affiliates in various countries worldwide. Copyright © 2021 Miltenyi Biotec and/or its affiliates. All rights reserved. 130-124-683.02