PRCC Rabbit Pab Antibody

PRCC rabbit pAb antibody Catalog No : Source: Concentration : Mol.Wt. (Da): A20185 Rabbit 1 mg/ml 54010 Applications WB Reactivity Human Dilution WB 1：500-2000 Storage -20°C/1 year Specificity This antibody detects endogenous levels of PRCC at Human Source / Purification The antibody was affinity-purified from rabbit serum by affinity- chromatography using specific immunogen. Immunogen Synthesized peptide derived from human PRCC Uniprot No Q92733 Alternative names Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Clonality Polyclonal Isotype IgG Conjugation Background This gene encodes a protein that may play a role in pre-mRNA splicing. Chromosomal translocations (X;1)(p11;q21) that result in fusion of this gene to TFE3 (GeneID 7030) have been associated with papillary renal cell carcinoma. A PRCC-TFE3 fusion protein is expressed in affected carcinomas and is likely associated with altered gene transactivation. This fusion protein has also been associated with disruption of the cell cycle.[provided by RefSeq, Aug 2010], Other PRCC TPRC, PRCC Produtc Images： Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry E-P-ELISA-Peptide A AAB Biosciences Products www.aabsci.cn FOR RESEARCH USE ONLY. NOT FOR HUMAN OR DIAGNOSTIC USE. Species Cross-Reactivity Key: H-Human M-Mouse R-Rat Hm-Hamster Mk-Monkey Vir-Virus Mi-Mink C-Chicken Dm-D. melanogaster X-Xenopus Z-Zebrafish B-Bovine Dg-Dog Pg-Pig Sc-S. cerevisiae Ce-C. elegans Hr-Horse All-All Species Expected Trademarks All product names and trademarks are the property of their respective owners. Regulatory Disclaimer For life science research only. Not for use in diagnostic procedures. Contact and Support: To ask questions, solve problems, suggest enhancements and report new applications, please visit our Online Technical Support Site. To call, write, fax, or email us, please visit www.aabsci.c n , contact information will be displayed. A AAB Biosciences Products www.aabsci.cn FOR RESEARCH USE ONLY. NOT FOR HUMAN OR DIAGNOSTIC USE..

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Renal Cell Carcinoma – Detection of PRCC-TFE3 and Alpha-TFEB Gene Fusions Using RT-PCR on Tissues (Odes 60153 and 60154) Notice of Assessment
Renal Cell Carcinoma – Detection of PRCC-TFE3 and Alpha-TFEB Gene Fusions Using RT-PCR on Tissues (odes 60153 and 60154) Notice of Assessment June 2013 DISCLAIMER: This document was originally drafted in French by the Institut national d'excellence en santé et en services sociaux (INESSS), and that version can be consulted at http://www.inesss.qc.ca/fileadmin/doc/INESSS/Analyse_biomedicale/Juin_2013/INESSS_Analyse_15.pdf http://www.inesss.qc.ca/fileadmin/doc/INESSS/Analyse_biomedicale/Juin_2013/INESSS_Analyse_16.pdf It was translated into English by the Canadian Agency for Drugs and Technologies in Health (CADTH) with INESSS’s permission. INESSS assumes no responsibility with regard to the quality or accuracy of the translation. While CADTH has taken care in the translation of the document to ensure it accurately represents the content of the original document, CADTH does not make any guarantee to that effect. CADTH is not responsible for any errors or omissions or injury, loss, or damage arising from or relating to the use (or misuse) of any information, statements, or conclusions contained in or implied by the information in this document, the original document, or in any of the source documentation. 1 GENERAL INFORMATION 1.1 Requestor: Centre hospitalier universitaire de Québec (CHUQ). 1.2 Application Submitted: August 1, 2012. 1.3 Notice Issued: April 12, 2013. Note: This notice is based on the scientific and commercial information (submitted by the requestor[s]) and on a complementary review of the literature according to the data available at the time that this test was assessed by INESSS. 2 TECHNOLOGY, COMPANY, AND LICENCE(S) 2.1 Name of the Technology Reverse transcription of messenger RNA and amplification (RT-PCR).
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Metastatic Tfe3-Overexpressing Renal Cell Carcinoma
ISSN: 2378-3419 Ribeiro et al. Int J Cancer Clin Res 2021, 8:148 DOI: 10.23937/2378-3419/1410148 Volume 8 | Issue 2 International Journal of Open Access Cancer and Clinical Research CASe RePoRt Metastatic Tfe3-Overexpressing Renal Cell Carcinoma: Case Report and Literature Review Paulo Victor Zattar Ribeiro1, Leonora Zozula Blind Pope2, Beatriz Granelli1, Milena Luisa Schulze1*, Andréa Rodrigues Cardovil Pires3 and Mateus da Costa Hummelgen1 1University of Joinville’s Region, UNIVILLE, Brazil 2Dona Helena Hospital, Blumenau Street, Brazil Check for 3Diagnostic Medicine Fonte, São Sebastião, Brazil updates *Corresponding author: Milena Luisa Schulze, Department of Medicine, University of Joinville’s Region, UNIVILLE, Paulo Malschitzki Street, 10 - Zona Industrial Norte, 89249-710 Joinville – SC, Brazil Abstract Introduction Background: Renal cell carcinoma (RCC) associated with Renal cell carcinoma (RCC) associated with Xp11.2 Xp11.2 translocation/TFE3 gene fusion (Xp11.2 RCC) is a translocation/TFE3 gene fusion (Xp11.2 RCC) is a rare rare subtype of RCC which is delineated as a distinct entity subtype of RCC which is delineated as a distinct enti- in the 2004 World Health Organization renal tumor classi- fication. ty in the 2004 World Health Organization renal tumor classification. Its morphology and clinical manifesta- Objective: To highlight a rare case, with few publications tions often overlap with those of conventional RCCs [1]. on the topic, in addition to providing scientific explanations about it. Children are more affected by this subtype than adults, accounts for 20-40% of pediatric RCC and 1-1.6% of RCC Method: This is a case report of a 58-year-old white male with the diagnosis of renal clear cell carcinoma (RCC).
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Epigenetic Alterations of Chromosome 3 Revealed by Noti-Microarrays in Clear Cell Renal Cell Carcinoma
Hindawi Publishing Corporation BioMed Research International Volume 2014, Article ID 735292, 9 pages http://dx.doi.org/10.1155/2014/735292 Research Article Epigenetic Alterations of Chromosome 3 Revealed by NotI-Microarrays in Clear Cell Renal Cell Carcinoma Alexey A. Dmitriev,1,2 Evgeniya E. Rudenko,3 Anna V. Kudryavtseva,1,2 George S. Krasnov,1,4 Vasily V. Gordiyuk,3 Nataliya V. Melnikova,1 Eduard O. Stakhovsky,5 Oleksii A. Kononenko,5 Larissa S. Pavlova,6 Tatiana T. Kondratieva,6 Boris Y. Alekseev,2 Eleonora A. Braga,7,8 Vera N. Senchenko,1 and Vladimir I. Kashuba3,9 1 Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia 2 P.A. Herzen Moscow Oncology Research Institute, Ministry of Healthcare of the Russian Federation, Moscow 125284, Russia 3 Institute of Molecular Biology and Genetics, Ukrainian Academy of Sciences, Kiev 03680, Ukraine 4 Mechnikov Research Institute for Vaccines and Sera, Russian Academy of Medical Sciences, Moscow 105064, Russia 5 National Cancer Institute, Kiev 03022, Ukraine 6 N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow 115478, Russia 7 Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow 125315, Russia 8 Research Center of Medical Genetics, Russian Academy of Medical Sciences, Moscow 115478, Russia 9 DepartmentofMicrobiology,TumorandCellBiology,KarolinskaInstitute,17177Stockholm,Sweden Correspondence should be addressed to Alexey A. Dmitriev; alex [email protected] Received 19 February 2014; Revised 10 April 2014; Accepted 17 April 2014; Published 22 May 2014 Academic Editor: Carole Sourbier Copyright © 2014 Alexey A. Dmitriev et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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TFE3 Antibody (C-Term) Affinity Purified Rabbit Polyclonal Antibody (Pab) Catalog # Ap18317b
10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 TFE3 Antibody (C-term) Affinity Purified Rabbit Polyclonal Antibody (Pab) Catalog # AP18317b Specification TFE3 Antibody (C-term) - Product Information Application WB,E Primary Accession P19532 Other Accession Q64092, Q05B92, NP_006512 Reactivity Human, Mouse Predicted Bovine Host Rabbit Clonality Polyclonal Isotype Rabbit Ig Calculated MW 61521 Antigen Region 489-516 TFE3 Antibody (C-term) - Additional Information TFE3 Antibody (C-term) (Cat. #AP18317b) western blot analysis in mouse kidney tissue Gene ID 7030 lysates (35ug/lane).This demonstrates the TFE3 Antibody detected the TFE3 protein Other Names (arrow). Transcription factor E3, Class E basic helix-loop-helix protein 33, bHLHe33, TFE3, BHLHE33 TFE3 Antibody (C-term) - Background Target/Specificity The microphthalmia transcription This TFE3 antibody is generated from factor/transcription rabbits immunized with a KLH conjugated synthetic peptide between 489-516 amino factor E (MITF-TFE) family of basic acids from the C-terminal region of human helix-loop-helix leucine zipper TFE3. (bHLH-Zip) transcription factors includes four family members: Dilution MITF, TFE3, TFEB and TFEC. The TEF3 protein WB~~1:1000 encoded by this gene activates transcription through binding to the Format muE3 motif of the Purified polyclonal antibody supplied in PBS immunoglobulin heavy-chain enhancer. The with 0.09% (W/V) sodium azide. This TFEC protein forms antibody is purified through a protein A heterodimers with the TEF3 protein and column, followed by peptide affinity inhibits TFE3-dependent purification. transcription activation. The TEF3 protein interacts with Storage transcription regulators such as E2F3, SMAD3, Maintain refrigerated at 2-8°C for up to 2 and LEF-1, and is weeks.
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Atlas Journal
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Mouse Anti-Human Renal Cell Carcinoma Monoclonal Antibody, Clone JID768 (CABT-L2932) This Product Is for Research Use Only and Is Not Intended for Diagnostic Use
Mouse Anti-Human Renal Cell Carcinoma monoclonal antibody, clone JID768 (CABT-L2932) This product is for research use only and is not intended for diagnostic use. PRODUCT INFORMATION Product Overview This antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods. Specificity Human Renal Cell Carcinoma Isotype IgG Source/Host Mouse Species Reactivity Human Clone JID768 Conjugate Unconjugated Applications IHC Reconstitution The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining. The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range. Positive Control Renal Cell Carcinoma Format Liquid Size Predilut: 7ml; Concentrate: 100ul, 1ml. Positive control slides also available. Buffer Predilute: Antibody Diluent Buffer Concentrate: Tris Buffer, pH 7.3 - 7.7, with 1% BSA Preservative <0.1% Sodium Azide Storage Store at 2-8°C. Do not freeze. Ship Wet ice Warnings This antibody is intended for use in Immunohistochemical applications on formalinfixed paraffin- 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved embedded tissues (FFPE), frozen tissue sections and cell preparations. BACKGROUND Introduction Renal Cell Carcinoma (RCC), also known as a gurnistical tumor, is a cancer of the kidney that arises from the proximal renal tubule; it is the most prevalent type of kidney cancer in adults. Anti-Renal Cell Carcinoma detects a glycoprotein in the brush border of the proximal renal tubule, and is a useful tool for diagnosis of primary renal cell carcinomas and metastatic renal cell carcinomas.
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Research Article Epigenetic Alterations of Chromosome 3 Revealed by Noti-Microarrays in Clear Cell Renal Cell Carcinoma
Hindawi Publishing Corporation BioMed Research International Volume 2014, Article ID 735292, 9 pages http://dx.doi.org/10.1155/2014/735292 Research Article Epigenetic Alterations of Chromosome 3 Revealed by NotI-Microarrays in Clear Cell Renal Cell Carcinoma Alexey A. Dmitriev,1,2 Evgeniya E. Rudenko,3 Anna V. Kudryavtseva,1,2 George S. Krasnov,1,4 Vasily V. Gordiyuk,3 Nataliya V. Melnikova,1 Eduard O. Stakhovsky,5 Oleksii A. Kononenko,5 Larissa S. Pavlova,6 Tatiana T. Kondratieva,6 Boris Y. Alekseev,2 Eleonora A. Braga,7,8 Vera N. Senchenko,1 and Vladimir I. Kashuba3,9 1 Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia 2 P.A. Herzen Moscow Oncology Research Institute, Ministry of Healthcare of the Russian Federation, Moscow 125284, Russia 3 Institute of Molecular Biology and Genetics, Ukrainian Academy of Sciences, Kiev 03680, Ukraine 4 Mechnikov Research Institute for Vaccines and Sera, Russian Academy of Medical Sciences, Moscow 105064, Russia 5 National Cancer Institute, Kiev 03022, Ukraine 6 N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow 115478, Russia 7 Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow 125315, Russia 8 Research Center of Medical Genetics, Russian Academy of Medical Sciences, Moscow 115478, Russia 9 DepartmentofMicrobiology,TumorandCellBiology,KarolinskaInstitute,17177Stockholm,Sweden Correspondence should be addressed to Alexey A. Dmitriev; alex [email protected] Received 19 February 2014; Revised 10 April 2014; Accepted 17 April 2014; Published 22 May 2014 Academic Editor: Carole Sourbier Copyright © 2014 Alexey A. Dmitriev et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Impairment of MAD2B–PRCC Interaction in Mitotic Checkpoint Defective T(X;1)-Positive Renal Cell Carcinomas
Impairment of MAD2B–PRCC interaction in mitotic checkpoint defective t(X;1)-positive renal cell carcinomas Marian A. J. Weterman*†‡§, Jan J. M. van Groningen*‡, Leon Tertoolen¶, and Ad Geurts van Kessel* *Department of Human Genetics, University Medical Center Nijmegen, 6500 HB Nijmegen, The Netherlands; and ¶Hubrecht Laboratory, Netherlands Institute for Developmental Biology, 3584 CT Utrecht, The Netherlands Edited by Janet D. Rowley, University of Chicago Medical Center, Chicago, IL, and approved September 19, 2001 (received for review June 15, 2001) The papillary renal cell carcinoma (RCC)-associated (X;1)(p11;q21) were transfected with a bait plasmid carrying the PRCC coding translocation fuses the genes PRCC and TFE3 and leads to cancer by sequence, and consecutively with DNA of the target plasmids an unknown molecular mechanism. We here demonstrate that the containing cDNAs of a library of t(X;1)-positive tumor cells (13). mitotic checkpoint protein MAD2B interacts with PRCC. The PRCC- Transfected yeast cells were first selected for the presence of TFE3 fusion protein retains the MAD2B interaction domain, but this both bait and target vector, after which colonies were scraped interaction is impaired. In addition, we show that two t(X;1)- from the plates, titered, and replated on selective medium positive RCC tumor cell lines are defective in their mitotic check- (without Leu, Trp, His, or Ade) at a density at least 10-fold more point. Transfection of PRCCTFE3, but not the reciprocal product than was originally plated. These cells were then allowed to grow TFE3PRCC, disrupts the mitotic checkpoint in human embryonic for at least 5 days at 30°C.
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PRCC, the Commonest TFE3 Fusion Partner in Papillary Renal Carcinoma Is Associated with Pre-Mrna Splicing Factors
Oncogene (2001) 20, 178 ± 187 ã 2001 Nature Publishing Group All rights reserved 0950 ± 9232/01 $15.00 www.nature.com/onc PRCC, the commonest TFE3 fusion partner in papillary renal carcinoma is associated with pre-mRNA splicing factors YM Skalsky*,1, PM Ajuh2, C Parker1, AI Lamond2, G Goodwin1 and CS Cooper1 1Institute of Cancer Research, Molecular Carcinogenesis Section, The Haddow Laboratories, 15 Cotswold Road, Sutton, Surrey, SM2 5NG, UK; 2Department of Biochemistry, The University of Dundee, MSI/WTB Complex, Dow Street, Dundee, DD1 5EH, UK In papillary renal cell carcinomas the TFE3 transcription shown to contain the PRCC ± TFE3, PSF ± TFE3 or factor becomes fused to the PSF and NonO pre-mRNA NonO ± TFE3 fusions, but not always reciprocal splicing factors and most commonly to a protein of fusions, they are the protein structures that believed unknown function designated PRCC. In this study we to be involved in carcinogenesis. have examined the ability of the resulting PRCC ± TFE3 TFE3 is a transcription factor that was originally and NonO ± TFE3 fusions to activate transcription from identi®ed by its ability to bind the mE3 site in the IgH the plasminogen activator inhibitor-1 (PAI-1) promoter. enhancer (Beckmann et al., 1990). The 576aa protein The results show that only fusion to PRCC enhanced contains a basic helix ± loop ± helix domain involved in transcriptional activation, indicating that the ability to DNA binding and two transcriptional activation enhance the level of transcription from endogenous TFE3 domains: an N-terminal acidic activation domain and promoters is not a consistent feature of TFE3 fusions.
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