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ATLAS ANTIBODIES in NEUROSCIENCE Front Cover: Page 2 (24) IHC-IF Staining in Mouse Brain Using Anti-USP20 (HPA007008) TABLE of CONTENTS

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ATLAS ANTIBODIES in NEUROSCIENCE Front Cover: Page 2 (24) IHC-IF Staining in Mouse Brain Using Anti-USP20 (HPA007008) TABLE of CONTENTS ATLAS ANTIBODIES IN NEUROSCIENCE Front cover: Page 2 (24) IHC-IF staining in mouse brain using Anti-USP20 (HPA007008) TABLE OF CONTENTS Background - the Human Protein Atlas (4) Triple A Polyclonals and PrecisA Monoclonals (5) Antibody Panel for Neuroscience (6-7) Antibodies against proteins involved in Signaling (8-12) Antibodies as Neural Lineage Markers (13-15) Antibodies against proteins involved in Aging and Neurodegenerative Disor- ders, such as Alzheimer´s, Parkinson´s and Huntington´s Diseases (16-17) Antibodies against proteins involved in Developmental Processes (18-19) Antibodies on HPA Mouse Brain Atlas (20-22) References (23) Contact (24) Front cover: IHC-IF staining in mouse brain using Anti-USP20 (HPA007008) Page 3 (24) proteinatlas.org The Human Protein Atlas: a The Human Protein Atlas consists of In the Pathology Atlas data from 12 Characterization of the Human six separate parts, each focusing on a patients with malignant glioma tumor is particular aspect of the genome-wide available for each antibody. Proteome analysis of the human proteins: The Human Protein Atlas (HPA) is a In addition to IHC images, there are Swedish-based program initiated in • The Tissue Atlas shows the available immunofluorescence (ICC- 2003 with the aim to map all the human distribution of proteins across all IF) images from staining in U-251 MG proteins in cells, tissues and organs using major tissues and organs in the cells, useful for information about the integration of various omics technologies, human body. localization of the subcellular proteins. including antibody-based imaging, • The Cell Atlas shows the subcellular mass spectrometry-based proteomics, localization of proteins in single cells. The Brain Atlas transcriptomics and systems biology. • The Pathology Atlas shows the The Brain Atlas, part of the Human impact of protein levels for survival of Protein Altas, is an open-access database All the data in the knowledge resource patients with cancer. that explores the protein expression in is open access to allow scientists both in • The Brain Atlas explores the protein the mammalian brains: human, pig and academia and industry to freely access expression in the mammalian brain mouse. the data for exploration of the human by integration of data from three proteome. mammalian species: human, pig and In the Brain Atlas, you can explore which mouse. proteins are expressed in a particular The HPA project aims to present an • The Blood Atlas contributes with data part of the brain or explore where in the expression map of the complete human regarding the cell types and proteome brain your protein of interest is expressed. proteome. To accomplish this, highly of human blood. Each mammalian brain is divided into 10 specific Triple A polyclonal antibodies • The Metabolic Atlas enables the defined anatomical regions. The regional are developed against all protein-coding exploration of protein function and expression protein profile includes 1,710 human genes and protein profiling is gene expression in the context of the human brain samples, 119 pig brain established in a multitude of tissues and human metabolic network. cells using tissue arrays. The antibodies samples and 67 mouse brain samples. are tested in immunohistochemistry (IHC), The HPA project employes tissue The basic architecture and organization of Western blot (WB) analysis, protein array microarrays with samples from 44 different the brain as well as gene expression and assay and immunofluorescent based normal human tissues, 20 different cancer protein sequences are largely preserved confocal microscopy (ICC-IF). types and 44 different human cell lines. The throughout mammalian evolution. 44 normal tissues are present in triplicate The Human Protein Atlas program has samples and represent 82 different cell The complexity of the human brain, an already contributed to several thousands types. All the IHC images for the normal organ with many structures and cell types of publications in the field of human biology tissue have undergone pathology-based present both in the cortical and subcortical and disease and it is selected by the annotation of expression levels. organization ELIXIR (www.elixir-europe. areas, makes the protein expression org) as a European core resource due to profiles and classification challenging. its fundamental importance for a wider life Validation in Human Brain science community. The Human Protein Tissues and Cell Lines For this reason, a smaller brain, such as Atlas consortium is mainly funded by the IHC images from human cerebellum, the mouse brain, is a useful model to study Knut and Alice Wallenberg Foundation. hippocampus, lateral ventricle wall, and the mammalian nervous system using the cerebral cortex tissues are available for same antibodies as for the human brain the antibodies, as well as from stainings such as our Triple A Polyclonals and in the following brain cell lines: D341 Med, PrecisA Monoclonals primary antibodies. SH-SY5Y, U-138 MG, U-251 MG, U-87 MG. References Uhlén M et al. (2010) Towards a knowledge-based Human Protein Atlas. Nat Biotechnol 28(12):1248-50 Uhlén M et al. (2015) Tissue-based map of the human proteome. Science 347(6220):1260419 Uhlén M et al. (2017) A pathology atlas of the human cancer transcriptome. Science 357(6352) Thul PJ et al. (2017) A subcellular map of the human proteome. Science 356(6340): eaal3321 Page 4 (24) Triple A Polyclonals Triple A Polyclonals - PrESTs with the lowest homology to other The antibodies developed the Building Blocks of HPA human proteins are used as antigens. The uniqueness and low cross-reactivity and characterized within the of Triple A Polyclonals versus other Approval Human Protein Atlas project proteins are due to a thorough selection The approval of the Triple A Polyclonals of antigen regions, affinity purification on relies on a combined validation of the are made available to the the recombinant antigen, validation using experimental results using IHC, WB scientific community by Atlas several methods and a stringent approval or ICC-IF, from RNA sequencing and process. information obtained via bioinformatics Antibodies under the brand prediction methods and literature. Since name Triple A Polyclonals. Development the literature is often inconclusive, an The Triple A Polyclonals are developed important objective of the HPA project is against recombinant human Protein to generate paired antibodies with non- Epitope Signature Tags (PrESTs) overlapping epitopes towards the same of approximately 50 to 150 amino protein target, allowing the results and acids. These protein fragments are validation of one antibody to be used to designed, using proprietary software, validate the other one. to contain unique epitopes present in the native protein suitable for triggering Triple A Polyclonal catalog the generation of antibodies of high Today, there are more than 18,000 Triple specificity. This is achieved by a complete A Polyclonals and new antibodies are human genome scanning to ensure that added each year. PrecisA Monoclonals Atlas Antibodies also provide a selected Isotyping PrecisA Monoclonals are number of mouse monoclonal antibodies, All PrecisA Monoclonals antibodies are under the brand name PrecisA isotyped to allow for multiplexing using developed by Atlas Antibodies, Monoclonals. The PrecisA Monoclonals catalog is regularly expanding with new isotype-specific secondary antibodies. based on the knowledge from products every year. the Human Protein Atlas Hybridoma Cell Cultivation with careful antigen design Unique Features Atlas Antibodies use in-vitro methods Special care is taken in offering clones for the production scale-up phase and extended validation of recognizing unique non-overlapping thus replacing the use of mice for the antibody performance. With epitopes and/or isotypes. Using the production of ascites fluid. same stringent PrEST production precise epitope information process and characterization procedure Antibody Characterization following all monoclonals, as for the Triple A Polyclonals, the The characterization of PrecisA PrecisA Monoclonals offer outstanding Monoclonals starts with an extensive these precise, accurate and performance in approved applications, literature search to select the most targeted antibodies are denoted together with defined specificity, secured relevant and clinically significant tissues continuity, and stable supply. In general, to use for IHC characterization. PrecisA Monoclonals. they also permit high working dilutions and contribute to more standardized Often there is more than one tissue type assay procedures. displayed in the IHC application data for each antibody. In addition to positively Clone Selection stained tissue, a negative control tissue Functional characterization is performed staining is also displayed and if relevant, on a large number of ELISA positive cell clinical cancer tissue staining. supernatants to select the optimal clones for each application before subcloning The Western blot (WB) characterization and expansion of selected hybridomas. includes results from the endogenous human cell or tissue protein lysates or optionally recombinant full-length human Epitope Mapping protein lysates. Clones are epitope-mapped using synthetic overlapping peptides in a bead- Each PrecisA Monoclonal is thus supplied based array format for selection of clones with the most relevant characterization with non-overlapping epitopes only. data for its specific target. The product numbers of Triple A Polyclonals start
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