TECHNICAL DATA 3H SHEET
Research Use Only. Not for use in diagnostic procedures.
SODIUM BOROHYDRIDE, [ 3H]-
Product Number: NET023H
LOT SPECIFIC INFORMATION
3 2549904 NaB H4 Lot Number: ______
10.4 Specific Activity: ______Ci/mmol M.W. 37.8 H BNa 384.8 4 ______GBq/mmol
Production Date: ______08 -MAR -2019
PACKAGING : Crystalline solid in silanized ampoule under argon. Shipped ambient.
STABILITY AND STORAGE RECOMMENDATIONS :
Sodium borohydride, [ 3H]-, as the crystalline solid, should be stored in an inert atmosphere in the freezer for maximum stability. It has been demonstrated that the addition of sodium hydroxide will stabilize aqueous solutions of sodium borohydride at higher pH values (pH > 10) (1,2). There is minimal decomposition for approximately one week of the aqueous solution (pH > 10) with storage in an inert atmosphere at 4 oC. Stored at -20 oC, sodium borohydride, [ 3H]- in 0.01N sodium hydroxide is stable for three months. Stored at -80 oC, it is stable for six months.
SPECIFIC ACTIVITY RANGE: >5 Ci/mmol (>185 GBq/mmol)
PREPARATIVE PROCEDURE : Sodium borohydride, [ 3H]- is prepared by exchange of the unlabeled precursor with tritium.
SPECIAL INFORMATION :
1. The specific activity of this lot was determined as follows: Benzaldehyde was reduced with this lot to benzyl alcohol, [ 3H]- . The functional specific activity was determined by mass spectrometry to be ______Ci/mmol. Hence, the specific activity of this lot was calculated to be four times its functional specific activity, or ______Ci/mmol.
2. There are some differences in the physical properties of sodium borohydride, [ 3H]- from non-radioactive material. The solubility of sodium borohydride, [ 3H]- in organic solvents is less than that of the non-radioactive compound.
3. All radioactive sodium borohydride has some color. Material at about 100 mCi/mmol is slightly pink. As the specific activity increases, the color deepens to a dark purple. Solutions of the material are colorless.
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CAUTION: Sodium borohydride, [ 3H]- may release tritium gas under the following conditions:
1. Exposure to water and acids. 2. Exposure to the atmosphere. 3. Prolonged storage in its present container. 4. Exposure of solution to temperatures over -20 oC.
PROCEDURE FOR COUNTING SODIUM BOROHYDRIDE: Sodium borohydride is a very powerful reducing agent used to add hydrogen to a molecule. It is very reactive and unstable in acid and is relatively stable in alkali. It can be counted safely in alkaline Aquasol®.
1. To 5ml Aquasol in mini-vials add 200 µl of 1N NaOH to each vial.
2. Place vials in the dark for 60 minutes to let the chemiluminescence decay.
3. Place the vials in the counter and check the background in the tritium channel. The chemiluminescence should decay to less than twice the background.
4. Add 50 µl of the sodium borohydride solution to be assayed to each vial.
5. Place the samples in the counter, equilibrate and count as soon as possible. The samples must be counted within 2 hours (finished counting within that time). Preferably they should be treated as rush samples or with very short turnarounds.
REFERENCES:
1. Bragdon, R.W. U.S. Patent 2970114, 1961.
2. Sodium Borohydride, Jan. 1979, Thiokol/Ventran Division, Danvers, Mass.
3. Gahmberg, C.G. et al., Methods Membrane Biology, Vol. 7, p. 184 (1976).
HAZARD INFORMATION: WARNING: This product contains a chemical known to the state of California to cause cancer. 0886:112096
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