LYN Antibody (Center) Purified Rabbit Polyclonal Antibody (Pab) Catalog # AW5352

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Specification

LYN Antibody (Center) - Product Information

Application WB, IHC-P,E Primary Accession P07948 Reactivity Human Predicted Mouse, Rat Host Rabbit Clonality polyclonal Calculated MW H=59,56;M=59,56 ;Rat=59,56 KDa Isotype Rabbit Ig Antigen Source HUMAN

LYN Antibody (Center) - Additional Information

Gene ID 4067

Antigen Region All lanes : Anti-LYN Antibody 205-239 (Center)(AW5352) at 1/1000 dilution Lane 1: K562 whole cell lysates Lane 2: Raji whole Other Names cell lysates Lane 3: Ramos whole cell lysates Tyrosine-protein kinase Lyn, Lck/Yes-related Lysates/proteins at 20 µg per lane. novel protein tyrosine kinase, V-yes-1 Secondary Goat Anti-Rabbit IgG, Yamaguchi sarcoma viral related oncogene (H+L),Peroxidase conjugated at 1/10000 homolog, p53Lyn, p56Lyn, LYN, JTK8 dilution Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Dilution WB~~1:1000 IHC-P~~1:25

Target/Specificity This LYN antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 205-239 amino acids from the Central region of human LYN.

Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage Maintain refrigerated at 2-8°C for up to 2 Immunohistochemical analysis of weeks. For long term storage store at -20°C paraffin-embedded H. liver section using LYN

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in small aliquots to prevent freeze-thaw Antibody (Center)(Cat#AW5352). AW5352 cycles. was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was Precautions used as the secondary, followed by DAB LYN Antibody (Center) is for research use staining. only and not for use in diagnostic or therapeutic procedures. LYN Antibody (Center) - Background

LYN Antibody (Center) - Protein Information Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors Name LYN and plays an important role in the regulation of innate and adaptive immune responses, Synonyms JTK8 hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also Function responses to DNA damage and genotoxic Non-receptor tyrosine-protein kinase that agents. Functions primarily as negative transmits signals from cell surface regulator, but can also function as activator, receptors and plays an important role in the depending on the context. Required for the regulation of innate and adaptive immune initiation of the B-cell response, but also for its responses, hematopoiesis, responses to down- regulation and termination. Plays an growth factors and cytokines, integrin important role in the regulation of B-cell signaling, but also responses to DNA differentiation, proliferation, survival and damage and genotoxic agents. Functions apoptosis, and is important for immune primarily as negative regulator, but can also self-tolerance. Acts downstream of several function as activator, depending on the immune receptors, including the B-cell context. Required for the initiation of the receptor, CD79A, CD79B, CD5, CD19, CD22, B-cell response, but also for its FCER1, FCGR2, FCGR1A, TLR2 and TLR4. Plays down-regulation and termination. Plays an a role in the inflammatory response to important role in the regulation of B-cell bacterial lipopolysaccharide. Mediates the differentiation, proliferation, survival and responses to cytokines and growth factors in apoptosis, and is important for immune hematopoietic progenitors, platelets, self-tolerance. Acts downstream of several erythrocytes, and in mature myeloid cells, such immune receptors, including the B-cell as dendritic cells, neutrophils and eosinophils. receptor, CD79A, CD79B, CD5, CD19, CD22, FCER1, FCGR2, FCGR1A, TLR2 and TLR4. Acts downstream of EPOR, KIT, MPL, the Plays a role in the inflammatory response to chemokine receptor CXCR4, as well as the bacterial lipopolysaccharide. Mediates the receptors for IL3, IL5 and CSF2. Plays an responses to cytokines and growth factors important role in integrin signaling. Regulates in hematopoietic progenitors, platelets, cell proliferation, survival, differentiation, erythrocytes, and in mature myeloid cells, migration, adhesion, degranulation, and such as dendritic cells, neutrophils and cytokine release. Down- regulates signaling eosinophils. Acts downstream of EPOR, KIT, pathways by phosphorylation of MPL, the chemokine receptor CXCR4, as immunoreceptor tyrosine-based inhibitory well as the receptors for IL3, IL5 and CSF2. motifs (ITIM), that then serve as binding sites Plays an important role in integrin signaling. for phosphatases, such as PTPN6/SHP-1, Regulates cell proliferation, survival, PTPN11/SHP-2 and INPP5D/SHIP-1, that differentiation, migration, adhesion, modulate signaling by dephosphorylation of degranulation, and cytokine release. kinases and their substrates. Phosphorylates Down-regulates signaling pathways by LIME1 in response to CD22 activation. phosphorylation of immunoreceptor Phosphorylates BTK, CBL, CD5, CD19, CD72, tyrosine-based inhibitory motifs (ITIM), that CD79A, CD79B, CSF2RB, DOK1, HCLS1, then serve as binding sites for LILRB3/PIR-B, MS4A2/FCER1B, PTK2B/PYK2, phosphatases, such as PTPN6/SHP-1, SYK and TEC. Promotes phosphorylation of PTPN11/SHP-2 and INPP5D/SHIP-1, that SIRPA, PTPN6/SHP-1, PTPN11/SHP-2 and modulate signaling by dephosphorylation of INPP5D/SHIP-1. Mediates phosphorylation of kinases and their substrates.

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Phosphorylates LIME1 in response to CD22 the BCR-ABL fusion protein. Required for rapid activation. Phosphorylates BTK, CBL, CD5, phosphorylation of FER in response to FCER1 CD19, CD72, CD79A, CD79B, CSF2RB, activation. Mediates KIT phosphorylation. Acts DOK1, HCLS1, LILRB3/PIR-B, as an effector of EPOR (erythropoietin MS4A2/FCER1B, SYK and TEC. Promotes receptor) in controlling KIT expression and may phosphorylation of SIRPA, PTPN6/SHP-1, play a role in erythroid differentiation during PTPN11/SHP-2 and INPP5D/SHIP-1. Mediates the switch between proliferation and phosphorylation of the BCR-ABL fusion maturation. Depending on the context, protein. Required for rapid phosphorylation activates or inhibits several signaling of FER in response to FCER1 activation. cascades. Regulates phosphatidylinositol 3- Mediates KIT phosphorylation. Acts as an kinase activity and AKT1 activation. Regulates effector of EPOR (erythropoietin receptor) in activation of the MAP kinase signaling cascade, controlling KIT expression and may play a including activation of MAP2K1/MEK1, role in erythroid differentiation during the MAPK1/ERK2, MAPK3/ERK1, MAPK8/JNK1 and switch between proliferation and maturation. Depending on the context, MAPK9/JNK2. Mediates activation of STAT5A activates or inhibits several signaling and/or STAT5B. Phosphorylates LPXN on cascades. Regulates phosphatidylinositol 3- 'Tyr- 72'. kinase activity and AKT1 activation. Regulates activation of the MAP kinase LYN Antibody (Center) - References signaling cascade, including activation of MAP2K1/MEK1, MAPK1/ERK2, MAPK3/ERK1, Yamanashi Y.,et al.Mol. Cell. Biol. MAPK8/JNK1 and MAPK9/JNK2. Mediates 7:237-243(1987). activation of STAT5A and/or STAT5B. Rider L.G.,et al.Gene 138:219-222(1994). Phosphorylates LPXN on 'Tyr-72'. Kinase Partanen J.,et al.Proc. Natl. Acad. Sci. U.S.A. activity facilitates TLR4-TLR6 87:8913-8917(1990). heterodimerization and signal initiation. Bielke W.,et al.Biochem. Biophys. Res. Phosphorylates SCIMP on 'Tyr-107'; this Commun. 186:1403-1409(1992). enhances binding of SCIMP to TLR4, Roifman C.M.,et al.Biochem. Biophys. Res. promoting the phosphorylation of TLR4, and Commun. 194:222-225(1993). a selective cytokine response to lipopolysaccharide in macrophages (By similarity). Phosphorylates CLNK (By similarity).

Cellular Location Cell membrane. Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Golgi apparatus. Membrane; Lipid- anchor. Note=Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport. Detected on plasma membrane lipid rafts

Tissue Location Detected in monocytes (at protein level). Detected in placenta, and in fetal brain, lung, liver and kidney. Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary

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neuroblastoma tumors.

LYN Antibody (Center) - Protocols

Provided below are standard protocols that you may find useful for product applications.

• Western Blot • Blocking Peptides • Dot Blot • Immunohistochemistry • Immunofluorescence • Immunoprecipitation • Flow Cytomety • Cell Culture

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