DOCSLIB.ORG

The Effects of Prostatic Fluid on Functional Characteristics of Cooled Canine Semen" (2015)

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
The Effects of Prostatic Fluid on Functional Characteristics of Cooled Canine Semen Louisiana State University LSU Digital Commons LSU Master's Theses Graduate School 2015 The ffecE ts of Prostatic Fluid on Functional Characteristics of Cooled Canine Semen Reto Fritsche Louisiana State University and Agricultural and Mechanical College, [email protected] Follow this and additional works at: https://digitalcommons.lsu.edu/gradschool_theses Part of the Veterinary Medicine Commons Recommended Citation Fritsche, Reto, "The Effects of Prostatic Fluid on Functional Characteristics of Cooled Canine Semen" (2015). LSU Master's Theses. 1906. https://digitalcommons.lsu.edu/gradschool_theses/1906 This Thesis is brought to you for free and open access by the Graduate School at LSU Digital Commons. It has been accepted for inclusion in LSU Master's Theses by an authorized graduate school editor of LSU Digital Commons. For more information, please contact [email protected]. THE EFFECTS OF PROSTATIC FLUID ON FUNCTIONAL CHARACTERISTICS OF COOLED CANINE SEMEN A Thesis Submitted to the Graduate Faculty of the Louisiana State University and Agricultural and Mechanical College in partial fulfillment of the requirements for the degree of Master of Science in The Interdepartmental Program in Veterinary Medical Sciences through the Department of Veterinary Clinical Sciences by Reto Fritsche Dr. med. vet., Vetsuisse Faculty University of Bern, 2007 August 2015 The author would like to dedicate this thesis to his family, who enabled him to reach out for the world and pursue his dreams and training far away from home. ii ACKNOWLEDGMENTS The author would like to sincerely thank Dr. Sara Lyle and Dr. Fiona Hollinshead for all their immeasurable support during the entire process of this research project and Dr. David Beehan for valuable input from before the start of this project until its completion. A further thank you to the third committee member Dr. Dale Paccamonti for his helpful questions regarding experimental design. I wish to thank Marilyn Dietrich for performing most of the flow cytometry data acquisition and her flexibility regarding appointments, also Dr. Jill Jenkins for the methodological input and her time and effort to introduce flow cytometry and fluorescent probes to me. Additional acknowledgement goes to Rita Perry for her work regarding supplies and laboratory support, to Micheal Kearney for performing the statistical part of this project and Maxon Graham for her help during sample collection and moral support. Last but not least, the author acknowledges the LSU VCS CORP grant for providing the funds for this study. iii TABLE OF CONTENTS ACKNOWLEDGMENTS …………………………………………………………………………………………………………….iii LIST OF TABLES ………………………………………………………………………………………………………..……………….vi LIST OF FIGURES ………………………………………………………………………………………………..…………………….vii ABSTRACT ………………………………………………………………………………………………………………………………….x CHAPTER 1 INTRODUCTION ...........................................................................................................1 CHAPTER 2 LITERATURE REVIEW ...................................................................................................4 2.1. Canine semen .........................................................................................................................4 2.2. Ejaculation ..............................................................................................................................4 2.3. Semen collection ....................................................................................................................6 2.4. Sperm transport in the bitch reproductive tract ....................................................................7 2.5. Prostatic fluid ..........................................................................................................................9 2.5.1. Characteristics and constituents ..............................................................................9 2.5.2. Effects of PF on semen ...........................................................................................14 2.5.2.1. Effects of PF on in vitro seminal parameters ..........................................14 2.5.2.2. Effects of PF on semen in vivo..................................................................18 2.5.3. Effects of seminal fluids in other species ...............................................................19 2.5.4. Summary of PF effects in dogs ...............................................................................21 2.6. Fertility ..................................................................................................................................22 2.6.1. Stud dog fertility ....................................................................................................22 2.6.2. Effect of prostatic disease on fertility.....................................................................23 2.7. Semen evaluation..................................................................................................................24 2.7.1. Motility ...................................................................................................................24 2.7.2. Morphology ............................................................................................................25 2.7.3. Concentration ........................................................................................................26 2.7.4. Ancillary semen tests .............................................................................................28 2.7.4.1. Plasma membrane integrity ....................................................................28 2.7.4.2. Acrosome integrity ..................................................................................30 2.7.4.3. Sperm chromatin structure assay (SCSA) ................................................31 2.8. Cooling of canine semen .......................................................................................................33 2.9. Cryopreservation ..................................................................................................................36 2.9.1. Ice crystal formation ..............................................................................................37 2.9.2. Osmotic stress ........................................................................................................37 2.9.3. Reactive oxygen species .........................................................................................39 2.9.4. Apoptotic-like changes ...........................................................................................40 iv CHAPTER 3 THE EFFECTS OF PROSTATIC FLUID ON FUNCTIONAL CHARACTERISTICS OF COOLED CANINE SEMEN ............................................................................................................................42 3.1. Introduction ..……………………………………………………………………………………………………………………42 3.2. Materials and methods .......……………………………………………………………………………………………..42 3.2.1. Semen and PF collection ........................................................................................43 3.2.2. Semen processing overview ..................................................................................44 3.2.3. Concentration ........................................................................................................46 3.2.4. Morphology ............................................................................................................46 3.2.5. pH ...........................................................................................................................46 3.2.6. Cryopreservation ...................................................................................................46 3.2.7. Motility .......…………………………………………………………………………………………….…………47 3.2.8. Hypo-osmotic swelling test (HOST) ........................................................................47 3.2.9. Flow cytometry ......................................................................................................48 3.2.9.1. Plasma membrane stability .....................................................................48 3.2.9.2. Acrosome reactivity ................................................................................49 3.2.9.3. Sperm chromatin structure assay (SCSA) ................................................50 3.2.10. Statistical analysis ................................................................................................51 3.3. Results ……………………………………………………………………………………………………………………………..52 3.4. Discussion .............................................................................................................................63 CHAPTER 4 CONCLUSIONS ...........................................................................................................74 REFERENCES .................................................................................................................................76 APPENDIX A THE CASA SPERM VISION® 3.0. PARAMETERS USED FOR CANINE SPERMATOZOAL ANALYSIS .………………………………………………......……………………………………………………………….…………90 APPENDIX B FLOW CYTOMETRIC GATING .....…………………………………………………………………………..91 APPENDIX C PERMISSIONS FOR USE OF COPYRIGHTED MATERIAL..............................................95 VITA ..………………………………………………………………………………………………………………………………………96 v LIST OF TABLES Table 2.1. Mean (and range if given) biochemical concentrations in first, second and third fraction from 3 dogs, post centrifugation (Adapted from Bartlett, 1962b).....……………….…………11 Table 2.2. Mean (± SD) biochemical
Load more

Recommended publications
  • Papazoglou-Mar PV Review 02
    204 Vol. 24, No. 3 March 2002 Comments? Questions? Email: [email protected] Web: VetLearn.com • Fax: 800-556-3288 CE Article #2 (1.5 contact hours) Refereed Peer Review Surgical Conditions of the Canine Penis and Prepuce KEY FACTS Aristotle University of Thessaloniki I Many penile and preputial Thessaloniki, Greece abnormalities are hereditary; Lysimachos G. Papazoglou, DVM, PhD, MRCVS trauma is the main cause of George M. Kazakos, DVM acquired defects. I Dogs that have one defect should ABSTRACT: Abnormalities of the canine penis and prepuce may have congenital or acquired be examined (especially in the causes. Diagnosis is based mainly on physical examination of the external genitalia. Treatment midline) for the presence of other of these abnormalities may require surgical intervention or medical management. Because abnormalities. many of the conditions may be hereditary, normal breeding is discouraged; therefore, surgical treatment (whether emergency or elective) should be aimed at repairing urinary rather than I Emergency surgery is often reproductive function. required in cases of traumatic abnormalities to treat or prevent urinary dysfunction or ongenital and acquired penile and preputial abnormalities have been 1–3 reproductive failure. described in dogs. Trauma is the main cause of acquired abnormali- Cties. Dogs with congenital or acquired abnormalities may be either asymptomatic or have urinary dysfunction or breeding failure. Dogs that have one defect should be examined thoroughly (especially in the midline) for the presence of others.1–3 Because many penile and preputial defects are hereditary, normal breeding should be discouraged; therefore, surgical intervention of congenital defects should be aimed at correcting or preventing urinary dysfunction rather than restoring reproductive performance.
    [Show full text]
  • Reading List.Pdf
    Reading list Nephrology / Urology 2013 American Journal of Veterinary Research Am J Vet Res. 2013 Jan;74(1):70‐7. doi: 10.2460/ajvr.74.1.70. Use of quantitative contrast‐enhanced ultrasonography to detect diffuse renal changes in Beagles with iatrogenic hypercortisolism. Haers H, Daminet S, Smets PM, Duchateau L, Aresu L, Saunders JH. Objective‐To determine the feasibility of quantitative contrast‐enhanced ultrasonography (CEUS) for detection of changes in renal blood flow in dogs before and after hydrocortisone administration. Animals‐11 Beagles Procedure‐Dogs were randomly assigned to 2 treatment groups: oral administration of hydrocortisone (9.6 mg/kg; n = 6) or a placebo (5; control group) twice a day for 4 months, after which the dose was tapered until treatment cessation at 6 months. Before treatment began and at 1, 4, and 6 months after, CEUS of the left kidney was performed by IV injection of ultrasonography microbubbles. Images were digitized, and time‐intensity curves were generated from regions of interest in the renal cortex and medulla. Changes in blood flow were determined as measured via contrast agent (baseline [background] intensity, peak ntensity, area under the curve, arrival time of contrast agent, time‐to‐peak intensity, and speed of contrast agent transport). Results‐Significant increases in peak intensity, compared with that in control dogs, were observed in the renal cortex and medulla of hydrocortisone‐treated dogs 1 and 4 months after treatment began. Baseline intensity changed similarly. A significant increase from control values was also apparent in area under the curve for the renal cortex 4 months after hydrocortisone treatment began and in the renal medulla 1 and 4 months after treatment began.
    [Show full text]
  • Imaging of Canine Neoplastic Reproductive Disorders
    animals Review Imaging of Canine Neoplastic Reproductive Disorders Marco Russo 1,* , Gary C.W. England 2, Giuseppe Catone 3 and Gabriele Marino 3,* 1 Department of Veterinary Medicine and Animal Production, University of Naples, Federico II, 80137 Naples, Italy 2 Sutton Bonington Campus, School of Veterinary Medicine and Science, University of Nottingham, Loughborough LE12 5RD, UK; [email protected] 3 Department of Veterinary Sciences, University of Messina, 98168 Messina, Italy; [email protected] * Correspondence: [email protected] (M.R.); [email protected] or [email protected] (G.M.) Simple Summary: The diagnosis of canine reproductive neoplasia remains challenging as none of the routinely performed diagnostic methods appear to have sufficient sensitivity or specificity. In recent years, advanced imaging techniques have been successfully performed in small animals; however, even though the incidence of reproductive neoplasia is high, no data are available on the performance of these techniques. This review evaluates the applicability of various diagnostic imaging modalities in dogs and describes the findings and specific patterns that may characterise different tumour types. Lamentably, some of the advanced imaging techniques have not yet been adopted as first-line diagnostic tools, although it is clear that in the future they will become important methods for the detection of male and female reproductive neoplasia. Abstract: Diagnostic imaging plays an essential role in the diagnosis and management of reproductive neoplasia in dogs and cats. The initial diagnosis, staging, and planning of surgical and radiation treatment and the response to therapy all involve imaging to varying degrees. Routine radiographs, Citation: Russo, M.; England, ultrasound, nuclear medicine, and cross-sectional imaging in the form of computed tomography G.C.W.; Catone, G.; Marino, G.
    [Show full text]
  • Law of Urination: All Mammals Empty Their Bladders Over the Same Duration
    Law of Urination: all mammals empty their bladders over the same duration Patricia J. Yang1, Jonathan C. Pham1, Jerome Choo1 and David L. Hu1;2∗ Schools of Mechanical Engineering1 and Biology2 Georgia Institute of Technology, Atlanta, GA 30332, USA Corresponding author: David L. Hu 801 Ferst Drive, MRDC 1308, Atlanta, GA 30332-0405 (404)894-0573 [email protected] Keywords: bladder, urology, allometry arXiv:1310.3737v3 [physics.flu-dyn] 26 Mar 2014 1 Abstract Many urological studies rely upon animal models such as rats and pigs whose urination physics and correlation to humans are poorly understood. Here we elucidate the hydrodynamics of urination across five orders of magnitude in animal mass. Using high-speed videography and flow-rate mea- surement at Zoo Atlanta, we discover the \Law of Urination," which states animals empty their bladders over nearly constant duration of 21 ± 13 seconds. This feat is made possible by larger animals having longer urethras, thus higher gravitational force and flow speed. Smaller mammals are challenged during urination due to high viscous and surface tension forces that limit their urine to single drops. Our findings reveal the urethra constitutes as a flow-enhancing device, enabling the urinary system to be scaled up without compromising its function. This study may help in the diagnosis of urinary problems in animals and in inspiring the design of scalable hydrodynamic systems based on those in nature. Significance Statement Animals eject liquid into environment for waste elimination, communication, and defense from predators. These diverse systems all rely on the fundamental principles of fluid mechanics, which have allowed us to make predictions about urination across a wide range of mammal sizes.
    [Show full text]
  • Jurnal Veterinar Malaysia
    Jurnal Veterinar alaysia ISSN 0128-2506 M Vol. 31 No. 2 (Dec) 2019 Veterinary Association Malaysia J. Vet. Malaysia (2019) 31 (1): 19-22 Case Reports ELECTROCAUTERY RESECTION AS A TREATMENT OPTION FOR CANINE TRANSMISSIBLE VENEREAL TUMOUR (CTVT) S. K. Rajendren1,2, K. Krishnan1, T. N. Ganesh1,2, N. S. Roslan2, N. A. Hashim2 and M. A. Mohamad2 1Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Pengkalan Chepa, Malaysia 2University Veterinary Clinic, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Pengkalan Chepa, Malaysia SUMMARY A 5-year-old Mongrel was brought presented with the complaint of having serosanguineous discharge from penis for a month since adoption. Physical examination revealed cauliflower-like mass at the bulbus glandis. Presence of numerous anisokaryotic and anisocytotic round to oval histiocytes with multivacuolated cytoplasm from cytology, an evidence of canine transmissibale venereal tumour (CTVT). The mass was successfully surgically resected using electrocautery and was in remission for 12 months (since January 2019). Keyword: Canine transmissible venereal tumour, surgical resection, electrocautery INTRODUCTION CASE REPORT Canine transmissible venereal tumour (CTVT) is a A 5-year-old male intact rescued stray, weighing round cell origin, histiocytic tumour that can be 19.6 kg was presented to UMK veterinary clinic horizontally transmitted among canidae family through (UMKVC, Kota Bharu, Kelantan) with the complaint of coitus, licking, biting and sniffing the tumour presented having serosanguineous discharge from the penis for one areas (Withrow, 2013; Da Silva et al., 2014). CTVT is month. The dog was on doxycycline (10mg/kg per os, also known as transmissible venereal granuloma, q24h for 28 days) for babesiosis.
    [Show full text]
  • Asistirana Reprodukcija Psov
    UNIVERZA V LJUBLJANI VETERINARSKA FAKULTETA Primož Klinc ASISTIRANA REPRODUKCIJA PSOV LJUBLJANA, 2020 1 Primož Klinc ASISTIRANA REPRODUKCIJA PSOV Izdala in založila UNIVERZA V LJUBLJANI Veterinarska fakulteta Strokovna recenzija: prof. dr. Marjan Kosec, dr. vet. med., VF, Univerza v Ljubljani izr. prof. dr. Tomaž Snoj, dr. vet. med., VF, Univerza v Ljubljani Lektoriranje: ga. Ivanka Šircelj Žnidaršič Grafična priprava: g. Božo Pirc CIP – kataloški zapis o publikaciji Kataložni zapis o publikaciji (CIP) pripravili v Narodni in univerzitetni knjižnici v Ljubljani COBISS.SI-ID=43530755 ISBN 978-961-95242-0-6 (pdf) 2 Vsebina UVOD ..................................................................................................................................................... 4 1 ODVZEM IN KONZERVIRANJE SEMENA .......................................................................................... 5 1.1 PREGLED PLEMENJAKA ........................................................................................................... 5 1.2 PRIDOBIVANJE SEMENA ......................................................................................................... 9 1.2.1 Odvzem semena s pomočjo digitalne manipulacije ...................................................... 9 1.2.2 Odvzem semena z elektroejakulatorjem ..................................................................... 11 1.2.3 Pridobivanje semena iz nadmodkov in semenovoda .................................................. 12 1.3 PREGLED EJAKULATA ...........................................................................................................
    [Show full text]
  • Diabetes and Male Sexual Function
    ORIGINAL ARTICLE Diabetes and male sexual function Khaleeq Rehman MBBS MS, Evette Beshay MD PhD, Serge Carrier MD FRCSC K Rehman, E Beshay, S Carrier. Diabetes and male sexual Diabète et fonctionnement sexuel chez les function. J Sex Reprod Med 2001;1(1):29-33. hommes Diabetes mellitus (DM) is the most common cause of erectile RÉSUMÉ : Le diabète sucré (DS) est la cause la plus fréquente de dysfunction (ED). Up to 28% of men complaining of ED have dysérection; il en est le principal facteur étiologique jusque dans 28 % des cas. Le DS devrait être envisagé chez tous les patients DM as the primary causative factor. DM should be considered qui consultent pour de la dysérection. Il faudrait inciter les in all patients presenting with ED. Known diabetic men hommes diabétiques à maîtriser parfaitement leur glycémie should be encouraged to have excellent control of their DM to pour éviter non seulement les complications chroniques du DS avoid not only the chronic complications of DM but also its mais aussi ses effets immédiats, notamment l'effet de l'hypergly- acute effects, especially the effect of hyperglycemia on erectile cémie sur le fonctionnement érectile. Il sera question, dans le function. The physiological impact of diabetes on sexual présent article, des répercussions physiologiques du diabète sur function in men is reviewed. le fonctionnement sexuel chez les hommes. Key Words: Diabetes mellitus; Ejaculation; Erectile dysfunction; Sexual function; Sexuality exuality is an important aspect in the lives of most indi- ED is usually progressive in these patients, with more than Sviduals and couples. Normal sexual function involves, 50% of diabetic men affected after 10 years of DM (3).
    [Show full text]
  • Sonoanatomical Studies on Penis of Dog (Canis Familiaris)
    Int.J.Curr.Microbiol.App.Sci (2019) 8(11): 1566-1572 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 8 Number 11 (2019) Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2019.811.181 Sonoanatomical Studies on Penis of Dog (Canis familiaris) V.A. Patel*, D.M. Bhayani and Harishbhai P. Gori Department of Veterinary Anatomy and Histology, College of Veterinary Science and A.H., AAU, Anand-388001, Gujarat, India *Corresponding author ABSTRACT The present study was conducted to establish normal ultrasonographic features of canine penis testis in 12 healthy male dogs, age ranging from 2 to 10 years. The echo texture of penis observed at pre scrotal and post K e yw or ds scrotal in transverse and longitudinal scan was done in non-erected penis. The ultrasonogram at the level of glans of penis showing a strong, Ultrasound, Penis, Sonoanatomical, hyperechogenic linear structure of os penis and above it the corpus Canine , Normal spongiosum visualized as a hypoechoic structure due to its acoustic shadow Article Info in longitudinal sonogram and hyperechoic round to ovoid shaped structure of os penis and round structure of urethra. The echogenicity of the os penis Accepted: 12 October 2019 formed „V‟ shaped structure and the urethra was located within a „V‟ Available Online: shaped groove on ventral aspect showing anechoic round structure. The 10 November 2019 sonoanatomical study revealed that the assessment of penis is easily performed and accurate for the clinical examination, helps to detection of small lesions or those which not accessible by palpation as well as to evaluate breeding soundness of dog.
    [Show full text]
  • Artificial Insemination in the Dog Larry Renze Iowa State University
    Volume 30 | Issue 1 Article 4 1968 Artificial Insemination in the Dog Larry Renze Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/iowastate_veterinarian Part of the Small or Companion Animal Medicine Commons Recommended Citation Renze, Larry (1968) "Artificial Insemination in the Dog," Iowa State University Veterinarian: Vol. 30 : Iss. 1 , Article 4. Available at: https://lib.dr.iastate.edu/iowastate_veterinarian/vol30/iss1/4 This Article is brought to you for free and open access by the Journals at Iowa State University Digital Repository. It has been accepted for inclusion in Iowa State University Veterinarian by an authorized editor of Iowa State University Digital Repository. For more information, please contact [email protected]. Artificial Insemination in the Dog by Larry Renze, Vet. Med. III A. REASONS FOR ARTIFICIAL manual manipulation, artificial vagina and INSEMINATION IN THE DOG electrical stimulation. In manual manipulation a bitch in es­ Artificial insemination means the col­ trum is placed in front of the male. When lecting of semen from a male and its sub­ the male exhibits sexual interest the pre­ sequent introduction into the genital pas­ putial skin is pushed caudally, exposing sage of a female. The first recorded arti­ one to two inches of the glans penis. The ficial insemination of dogs was done by base of the penis behind the bulbus glandis Abbe Spallanzani in 1780. The reasons for is grasped through the prepuce and slight artificial insemination in dogs may be pressure is applied by the fingers (see Fig. listed as; 1). Dogs can be made to ejaculate by ma- 1.
    [Show full text]
  • Preputial Reconstruction After Traumatic Avulsion in a Dog
    Austral Journal of Veterinary Sciences ISSN: 0719-8000 ISSN: 0719-8132 [email protected] Universidad Austral de Chile Chile Preputial reconstruction after traumatic avulsion in a dog Huppes, RR; Sprada, AG; Uscategui, RAR; Passos, BLS; De Nardi, AB; Pazzini, JM; Castro, JLC; Minto, BW; Vicente, WRR Preputial reconstruction after traumatic avulsion in a dog Austral Journal of Veterinary Sciences, vol. 48, no. 1, 2016 Universidad Austral de Chile, Chile Available in: http://www.redalyc.org/articulo.oa?id=501752373016 PDF generated from XML JATS4R by Redalyc Project academic non-profit, developed under the open access initiative Preputial reconstruction aer traumatic avulsion in a dog Reconstrucción prepucial posterior a avulsión traumática en canino RR Huppes Universidade de Uningá, Brasil AG Sprada Universidade Estadual Paulista, Brasil RAR Uscategui [email protected] Universidade Estadual Paulista, Brasil BLS Passos Clínica Veterinária Planeta Bicho, Brasil AB De Nardi Universidade Estadual Paulista, Brasil JM Pazzini Universidade Estadual Paulista, Brasil JLC Castro Austral Journal of Veterinary Sciences, vol. 48, no. 1, 2016 Pontifícia Universidade Católica do Paraná, Brasil Universidad Austral de Chile, Chile BW Minto Universidade Estadual Paulista, Brasil Accepted: 13 August 2015 WRR Vicente Redalyc: http://www.redalyc.org/ Universidade Estadual Paulista, Brasil articulo.oa?id=501752373016 Abstract: Extensive loss of preputial tissue can cause additional penile injuries and infections, therefore it should be treated promptly. Extensive lesions may require reconstrsuctive techniques and even partial or complete amputation of the penis. is paper report the case of male mongrel dog showing traumatic avulsion lesion in the pelvic limbs, inguinal and preputial region. An axial pattern flap from the limb that was later amputated due to severity of injury was used for the inguinal and preputial reconstruction.
    [Show full text]
  • Penile Hypoplasia and Rudimentary Prepuce in a Dog (78,XY; SRY-Positive): a Case Report
    Veterinarni Medicina, 61, 2016 (5): 279–287 Case Report doi: 10.17221/8884-VETMED Penile hypoplasia and rudimentary prepuce in a dog (78,XY; SRY-positive): a case report D. Rozanska1, I. Szczerbal2, M. Stachowiak2, P. Debiak1, A. Smiech1, P. Rozanski1, M. Orzelski1, B. Zylinska1, M. Switonski2, B. Slaska1 1University of Life Sciences in Lublin, Lublin, Poland 2Poznan University of Life Sciences, Poznan, Poland ABSTRACT: This is the first report in the literature concerning penile hypoplasia and rudimentary prepuce in a 78,XY; SRY-positive male that received a successful surgical treatment. A six-month-old male mixed-breed dog with body weight of 3.5 kg and with symptoms of prolonged stranguria and abnormalities of the external genitalia were presented by the owner at the University Surgical Clinic. Clinical, biochemical, radiological, pathological, and genetic examinations of the dog were carried out. Penile hypoplasia and rudimentary prepuce were diagnosed. Early diagnosis of penile hypoplasia and rudimentary prepuce in small animals requires a high level of vigilance and is based on clinical and ultrasonographical findings. The radiography revealed a fan-shaped widening of the caudal part and shortening of the os penis. A hyperechogenic os penis with a wide posterior part and a slightly curved, smooth anterior end was imaged. No normal prepuce structures were observed. The endocrinological examina- tion showed a substantially decreased testosterone level. Fast surgical intervention is preferable and confirms the diagnosis. In the presented case report enlargement of the preputial orifice was applied in order to prevent urinary retention and recurrent urinary tract infections. Orchiectomy was also performed.
    [Show full text]
  • Download This PDF File
    Journal of the Hellenic Veterinary Medical Society Vol. 68, 2017 Preputial reconstruction and urethrostomy after subtotal penile amputation in a dog KATAYAMA M Division of Small Animal Surgery, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan Iwate University Veterinary Teaching Hospital, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan SEKI T Iwate University Veterinary Teaching Hospital, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan TAKAHIRA A Iwate University Veterinary Teaching Hospital, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan Takahira Animal Hospital, 5-10-1 Takahashi, Tagajo, Miyagi 985-0853, Japan https://doi.org/10.12681/jhvms.16072 Copyright © 2018 M KATAYAMA, T SEKI, A TAKAHIRA To cite this article: KATAYAMA, M., SEKI, T., & TAKAHIRA, A. (2018). Preputial reconstruction and urethrostomy after subtotal penile amputation in a dog. Journal of the Hellenic Veterinary Medical Society, 68(4), 669-674. doi:https://doi.org/10.12681/jhvms.16072 http://epublishing.ekt.gr | e-Publisher: EKT | Downloaded at 25/09/2021 01:52:10 | Case report J HELLENIC VET MED SOC 2017, 68(4): 669-674 ΠΕΚΕ 2017, 68(4): 669-674 Κλινικό περιστατικό Preputial reconstruction and urethrostomy after subtotal penile amputation in a dog Katayama M., BVSc, MS, PhD1, 2, Seki T., BVSc2, Takei Y., BVSc, MS 2 Takahira A., BVSc2,3 1 Division of Small Animal Surgery, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan 2 Iwate University Veterinary Teaching Hospital, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan 3 Takahira Animal Hospital, 5-10-1 Takahashi, Tagajo, Miyagi 985-0853, Japan ABSTRACT.
    [Show full text]