AKTAIUMIA Akta Kimindo Vol 1January 2016: 1-4 INDONESIA

Flavonoid Compounds From The Leaves Of Tomentosa And Their Cytotoxic Activity Against P-388 Murine Leukemia Cell .

l Lilis Siti Aisyah*I,2" Yenny Febriani Yun ,2, Ade Akbar Abdilla \ Tati Herlina', Euis Julaeha', Unang Supratman,**'

J Natural Product Chemistry Group, Department of Chemlstry, The Faculty of Mathematics and Natural Sciences, Padjadjaran University, Bandung-Sumedang Km 2 J, Jatinangor, Sumedang 45363 2Department of Chemistry, The Faculty of Mathematics and Natural Sciences, Jenderal Achmad Yani University, Cimahi 40528

Corresponding author: * lies.svarifudin(£i1gmail.com , ** U [email protected] 3The Faculty of Pharmacy, Jenderal Achmad Yani University, Cimahi 40528

1. INTRODUCTION Abstract Kalanchoe , known as "sosor bebek" in Indonesia is a perennial herb and has succulent leaves. Kalanchoe tomentosa belongs to the family The plant is known in folklore and traditional medicine is a beatiful, perennial and succulent in Indonesia for the treatment of fever, abscesses, plant. Kalanchoe consists of about 130 bruises, contused wounds, coughs and skin diseases. species of annual and perennial shrubs. The plant During the course of our continuing search for novel ussually occur in semi desert of Saudia Arabia, cytotoxic compounds, the methanolic extract of Yemen, CentarlAfrica, Madagascar, tropical areas Kalanchoe tomentosa showed cytotoxic activity against P-388 murine leukemia cells. The methanolic of Asia, Australia, and tropical America. extract of the fresh leaves of K. tomentosa was Ussually, it is cultivated as garden ornamental and concentrated and extracted successively with n-hexane, sand gardens with a medium humidity", ethyl acetate and n-butanol. The ethyl acetate extract Kalanchoe plants are used as traditional exhibited strongest cytotoxic activity againts P-388 medicines to cure headache, cough, chest pain, murine leukemia cells. By using the cytotoxic activity ulcer, and other skin deseases. They overcome to follow the separation, the ethyl acetate fraction was fever, fix the irregular menstruation, heal wound separated by combination of column chromatography and boil, not only in Indonesia but also almost on silica gel and preparative TLC on silica gel GF to 254 everywhere in the world'", Some researches afford a kaempferol-3-0-glycosides (1) and reported that Kalanchoe plants contain kaempfero 1-3-0-rhamnoside (2). Compound 1 and 2 showed cytotoxic activity against P-388 murine bufadienolide':", triterpenoid" ,,and flavonoid't" leukemia cells with ICso > 100J.lglmLand ICso 3.32 Jig / and biological activities like antileismanial, mL. antiinflamatory, cytotoksic, and inhibiting tumor cell growth-l', One of unknown Kalanchoe plants Keywords: P-388 murine leukemia cells, kaempferol-3- ethnopharmacologically is Kalanchoe tomentosa, O-glycosides, Kaempferol-3-0- especially its anticancer activity. Cancer is a rhamnoside, Kalanchoe tomentosa disease that is becoming one of the major threats OH OH ::7 I ::7 I to health as it is the second cause of death after HO ::::,... HO ::::,... heart disease. In Indonesia reported increased 3 I ~OH ,"1',...-{'CH cancer deaths each year ranging 1.4% in 1972 to o 0 0;---UOH OH a HO HO OH OH 0 HO HO 4.4% in 199i~). In coping with cancer, various

2 efforts had been whom did seek anticancer compounds from plants. 1.

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