DOCSLIB.ORG

Correlation of Seasonal Nitrification Failure and Ammonia-Oxidizing Community Dynamics in a Wastewater Treatment Plant Treating Water from a Saline Thermal Spa

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Correlation of Seasonal Nitrification Failure and Ammonia-Oxidizing Community Dynamics in a Wastewater Treatment Plant Treating Water from a Saline Thermal Spa Ann Microbiol (2014) 64:1671–1682 DOI 10.1007/s13213-014-0811-5 ORIGINAL ARTICLE Correlation of seasonal nitrification failure and ammonia-oxidizing community dynamics in a wastewater treatment plant treating water from a saline thermal spa Luciano Beneduce & Giuseppe Spano & Francesco Lamacchia & Micol Bellucci & Francesco Consiglio & Ian M. Head Received: 21 August 2013 /Accepted: 9 January 2014 /Published online: 24 January 2014 # Springer-Verlag Berlin Heidelberg and the University of Milan 2014 Abstract In this work we evaluated the effect of a perturba- sampling period. This study reports a clear association of tion on nitrification performance in a wastewater treatment microbial community dynamics (strongly correlated to salin- plant (WWTP) treating urban and saline thermal bath waste- ity, temperature, and dissolved oxygen) to nitrification perfor- water, which regularly occurred during summer months. We mance. Particularly, ammonia-oxidizing bacteria are severely wanted to find out if this related to changes in the ammonia affected by drastic changes in operational conditions, with oxidizing communities. The bacterial and ammonia-oxidizing direct consequences on WWTP performance. bacterial (AOB) community from three different basins of the WWTP were evaluated using PCR-DGGE and cloning and Keywords Activated sludge . DGGE (denaturing gradient gel sequencing of 16S rRNA gene fragments, over a six month electrophoresis) . Diversity . Nitrification . AOB . Wastewater survey. Both eubacterial and AOB communities underwent treatment continuous change over time, with a particularly prominent shift between the third and fourth month of monitoring for eubacteria and the fourth month and fifth month for AOB. At Introduction the same time, reduction of nitrification performance was observed in the WWTP. The AOB community in the activated Nitrification in wastewater treatment plants (WWTP) is main- sludge was dominated by clones with high 16S rRNA se- ly driven by the activity of aerobic, chemolithoautotrophic quence identity to halophilic-halotolerant organisms from the ammonia oxidizing bacteria (AOB) and nitrite-oxidizing bac- Nitrosomonas oligotrohpa and Nitrosomonas marina clusters. teria (NOB). The former are typically considered rate limiting A significant correlation (R=0.97) was detected between the for nitrification, since AOB are slow growing and more sen- structure of the AOB community and environmental parame- sitive to environmental factors such as dissolved oxygen (DO) ters, indicating that the AOB community structure changed in content, salinity, light and substrate concentration (Wagner line with the environmental changes that took place over the et al. 1995; Koops et al. 2003; Geets et al. 2006). Failure of nitrogen removal from sewage in WWTP can dramatically : : L. Beneduce (*) G. Spano F. Lamacchia affect surface water environments receiving final effluent, Dipartimento di Scienze Agrarie, Alimentari ed Ambientali, leading to eutrophication and other ecological risks (Painter Università di Foggia, via Napoli 25, 71122 Foggia, Italy 1986; Philips et al. 2002;Jensen2003). e-mail: [email protected] AOB form two evolutionary distinct groups. The first falls : L. Beneduce M. Bellucci within the Gammaproteobacteria and comprises the STAR* Agroenergy Research Group, Università di Foggia, Foggia, Nitrosococcus species, and the second forms a monophyletic Italy group within the Betaproteobacteria, with the genera F. Consiglio Nitrosomonas and Nitrosospira (Purkhold et al. 2000). More Acquedotto Pugliese S.p.A. U.C.I.S. compartimento di Foggia, recently, ammonia-oxidizing Archaea have been detected in Foggia, Italy WWTP (Park et al. 2006); however, their significance in nitrogen removal in wastewater treatment remains open to I. M. Head School of Civil Engineering and Geosciences, Newcastle University, debate (Wells et al. 2009;YeandZhang2011). In addition, Drummond Building, Newcastle upon Tyne, NE1 7RU England, UK a further group belonging to the order Planctomycetales is 1672 Ann Microbiol (2014) 64:1671–1682 associated with the anaerobic ammonia oxidation (Anammox) conducted a survey of AOB communities and nitrification process (Jetten et al. 2001). performance over a 6-month period in which the salinity of The phylogeny of AOB has been described on the basis of the wastewater increased by 30 %. comparative 16S rRNA sequence analysis (Head et al. 1993; The aim of this study was to establish the effect of this Teske et al. 1994;Purkholdetal.2000). To date, all AOB perturbation on nitrification performance and whether this isolated from soil and freshwater habitats belongs to the related to changes in the AOB communities. During the 6- Betaproteobacteria, whereas AOB belonging to month survey the nitrogen removal performance was severely Gammaproteobacteria have only been isolated from marine compromised by environmental conditions. and other saline environments (Ward and O’Mullan 2002). Culture based studies of the diversity and ecology of AOB in natural and engineered systems are severely hampered by Materials and methods the slow growth rates and autotrophic metabolism of AOB, which are easily outcompeted by heterotrophic organisms Sampling (Watson et al. 1989; Wagner et al. 1993). Analysis of 16S rRNA genes and genes encoding the alpha Sludge samples were collected from an activated sludge subunit of ammonia monooxygenase (amoA), Fluorescence wastewater treatment plant, located in Margherita di Savoia In-Situ Hybridization (FISH) and denaturing gradient gel (Apulia, Italy), treating urban and saline spa wastewater. The electrophoresis (DGGE) have been used widely to analyse WWTP consisted of a primary settler, a pre-denitrification AOB populations and communities in many environments basin with recirculation from oxidation/nitrification, an acti- and have led to many aspects of the ammonia oxidizing vated sludge oxidation/nitrification basin and a secondary bacterial community ecology being unravelled in detail. settler. The average hydraulic retention time was 12.5 h and AOB community composition and diversity in natural and the average solid retention time was 8.4 days. engineered systems have been related to variables such as Sampling was conducted at intervals of 30 days, over a ammonia content (Lydmark et al. 2007), salinity (Freitag period of six months, from March to August. A total of six et al. 2006), type of substratum (Gorra et al. 2007), DO time-weighted (12 h) average samples were taken in triplicate concentrations (Bellucci et al. 2011), reactor design (Rowan from the pre-denitrification basin (DEN), oxidation basin et al. 2003) and the presence of heavy metals (Principi et al. (OXI) and recirculating sludge from the secondary settler 2009). Moreover, characterization of the AOB community (RIC), respectively. After sampling, sludge samples were composition and diversity, distribution patterns and ecophys- frozen at −20 °C and transported to the laboratory for DNA iology has been reviewed (Koops et al. 2003) while 16S extraction. The pH, conductivity and DO concentration were rRNA and amoA based phylogeny has been extended and determined directly at the time of sampling. Total suspended improved (Purkhold et al. 2003). solids (TSS), nitrite, nitrate and ammonium content were Correlations among the structure and functional stability of assayed on unfrozen aliquots of the same samples used for AOB communities in WWTPs, physicochemical parameters microbial community analysis. and efficiency of bioreactors have been postulated and inves- tigated by many authors (Wittebolle et al. 2005, 2008; DNA extraction and PCR amplification Siripong and Rittman 2007;Belluccietal.2013). However, many aspects still remain to be ascertained. For instance, the Ten milliliters of mixed liquor samples were centrifuged at role of functional redundancy, which may allow communities 10,000 g for 2 min and the supernatant was discarded. Nucleic to maintain physiological capabilities while their composition acids extraction from 0.25 g of sludge samples was carried out changes in response to environmental conditions, is not yet with a Power Soil DNA extraction kit (MoBio, Carlsbad, CA well understood. – USA) according to the manufacturer’s protocol. Each DNA In this paper we monitored bacterial and AOB community extraction was performed in duplicate, the amount of DNA composition and diversity in a wastewater plant treating a was estimated by visualization on a 1 % agarose gel stained mixture of urban wastewater and wastewater from a thermal with ethidium bromide, and the molecular weight was esti- bath (spa). mated by comparison with Gelpilot 100 bp plus ladder This WWTP is regularly subject to an increase in salinity, (Qiagen, Milan, Italy) used as a DNA molecular weight mark- temperature and reduced dissolved oxygen content every year er. Extracted DNA concentration was adjusted to 10 ng μl−1 during the summer months. The changes in salinity in the with sterile deionised water and stored at −20 °C until wastewater resulting from seasonal changes in the contribu- analysis. tion from the spa bath waters provide an excellent opportunity To study total Bacteria and AOB communities, a separate to assess the effects of short term salinity changes on AOB primer set and PCR protocols were used. PCR amplification communities and nitrification performance. We, therefore, of eubacterial 16S rRNA gene fragments was performed using Ann Microbiol (2014) 64:1671–1682 1673 Primer 357f-GC (5′-CGC CCG CCG CGC GCG GCG GGC acrylamide
Load more

Recommended publications
  • Development and Evaluation of Rrna Targeted in Situ Probes and Phylogenetic Relationships of Freshwater Fungi
    Development and evaluation of rRNA targeted in situ probes and phylogenetic relationships of freshwater fungi vorgelegt von Diplom-Biologin Christiane Baschien aus Berlin Von der Fakultät III - Prozesswissenschaften der Technischen Universität Berlin zur Erlangung des akademischen Grades Doktorin der Naturwissenschaften - Dr. rer. nat. - genehmigte Dissertation Promotionsausschuss: Vorsitzender: Prof. Dr. sc. techn. Lutz-Günter Fleischer Berichter: Prof. Dr. rer. nat. Ulrich Szewzyk Berichter: Prof. Dr. rer. nat. Felix Bärlocher Berichter: Dr. habil. Werner Manz Tag der wissenschaftlichen Aussprache: 19.05.2003 Berlin 2003 D83 Table of contents INTRODUCTION ..................................................................................................................................... 1 MATERIAL AND METHODS .................................................................................................................. 8 1. Used organisms ............................................................................................................................. 8 2. Media, culture conditions, maintenance of cultures and harvest procedure.................................. 9 2.1. Culture media........................................................................................................................... 9 2.2. Culture conditions .................................................................................................................. 10 2.3. Maintenance of cultures.........................................................................................................10
    [Show full text]
  • Response of Heterotrophic Stream Biofilm Communities to a Gradient of Resources
    The following supplement accompanies the article Response of heterotrophic stream biofilm communities to a gradient of resources D. J. Van Horn1,*, R. L. Sinsabaugh1, C. D. Takacs-Vesbach1, K. R. Mitchell1,2, C. N. Dahm1 1Department of Biology, University of New Mexico, Albuquerque, New Mexico 87131, USA 2Present address: Department of Microbiology & Immunology, University of British Columbia Life Sciences Centre, Vancouver BC V6T 1Z3, Canada *Email: [email protected] Aquatic Microbial Ecology 64:149–161 (2011) Table S1. Representative sequences for each OTU, associated GenBank accession numbers, and taxonomic classifications with bootstrap values (in parentheses), generated in mothur using 14956 reference sequences from the SILVA data base Treatment Accession Sequence name SILVA taxonomy classification number Control JF695047 BF8FCONT18Fa04.b1 Bacteria(100);Proteobacteria(100);Gammaproteobacteria(100);Pseudomonadales(100);Pseudomonadaceae(100);Cellvibrio(100);unclassified; Control JF695049 BF8FCONT18Fa12.b1 Bacteria(100);Proteobacteria(100);Alphaproteobacteria(100);Rhizobiales(100);Methylocystaceae(100);uncultured(100);unclassified; Control JF695054 BF8FCONT18Fc01.b1 Bacteria(100);Planctomycetes(100);Planctomycetacia(100);Planctomycetales(100);Planctomycetaceae(100);Isosphaera(50);unclassified; Control JF695056 BF8FCONT18Fc04.b1 Bacteria(100);Proteobacteria(100);Gammaproteobacteria(100);Xanthomonadales(100);Xanthomonadaceae(100);uncultured(64);unclassified; Control JF695057 BF8FCONT18Fc06.b1 Bacteria(100);Proteobacteria(100);Betaproteobacteria(100);Burkholderiales(100);Comamonadaceae(100);Ideonella(54);unclassified;
    [Show full text]
  • Microbial Community Response to Heavy and Light Crude Oil in the Great Lakes
    Microbial Community Response to Heavy and Light Crude Oil in the Great Lakes Stephen Techtmann 10/24/19 Microbial Sensors Techtmann Lab @ MTU Investigating the applications of environmental microbial communities Hydraulic Fracturing Related Antibiotic Resistance Oil Bioremediation Techtmann Lab @ MTU Overview • Background on oil biodegradation • Microbial response to light and heavy crude oil in the Great Lakes • Machine learning for prediction of contamination in the Great Lakes. Oil Spills Deepwater Horizon Enbridge Line 6B Deepwater Horizon Oil Spill • 4,1000,000 bbl of oil released • Light Sweet Crude oil released • April 20, 2010 • 1101.7 miles of shoreline oiled Atlas and Hazen 2011 Enbridge Line 6B Spill – Marshall MI • 20,082 bbl of oil released • Diluted Bitumen • July 26, 2010 • 70 miles of shoreline oiled https://www.mlive.com/news/kalamazoo/2010/07/state_of_emergency_declared_as.html Oil Transmissions Pipelines in the Great Lakes Region Line 5: • 645 miles from Superior WI to Sarnia Ontario • 540,000 barrels per day • Light crude and natural gas liquids (NGLs) Crude oil Oil types and API Gravity Microbes and Biotechnology (Bioremediation) Low cost input Microbe High value output Decreased Cost Contaminant Increased Efficiency Carbon dioxide or non- toxic daughter products Carbon dioxide Microbial Biomass Petroleum Microbe Daughter Products Water Microbial Ecology and Biotechnology Low cost input Microbe High value output Decreased Cost/Increased Efficiency Complex input Input A Microbe Microbe Output A Input B Microbe Output
    [Show full text]
  • Aquabacterium Gen. Nov., with Description of Aquabacterium Citratiphilum Sp
    International Journal of Systematic Bacteriology (1999), 49, 769-777 Printed in Great Britain Aquabacterium gen. nov., with description of Aquabacterium citratiphilum sp. nov., Aquabacterium parvum sp. nov. and Aquabacterium commune sp. nov., three in situ dominant bacterial species from the Berlin drinking water system Sibylle Kalmbach,’ Werner Manz,’ Jorg Wecke2 and Ulrich Szewzyk’ Author for correspondence : Werner Manz. Tel : + 49 30 3 14 25589. Fax : + 49 30 3 14 7346 1. e-mail : [email protected]. tu-berlin.de 1 Tech nisc he U nive rsit ;it Three bacterial strains isolated from biofilms of the Berlin drinking water Berlin, lnstitut fur system were characterized with respect to their morphological and Tec hn ischen Umweltschutz, Fachgebiet physiological properties and their taxonomic position. Phenotypically, the Okologie der bacteria investigated were motile, Gram-negative rods, oxidase-positive and Mikroorganismen,D-l 0587 catalase-negative, and contained polyalkanoates and polyphosphate as Berlin, Germany storage polymers. They displayed a microaerophilic growth behaviour and 2 Robert Koch-lnstitut, used oxygen and nitrate as electron acceptors, but not nitrite, chlorate, sulfate Nordufer 20, D-13353 Berlin, Germany or ferric iron. The substrates metabolized included a broad range of organic acids but no carbohydrates at all. The three species can be distinguished from each other by their substrate utilization, ability to hydrolyse urea and casein, cellular protein patterns and growth on nutrient-rich media as well as their temperature, pH and NaCl tolerances. Phylogenetic analysis, based on 165 rRNA gene sequence comparison, revealed that the isolates are affiliated to the /I1 -subclass of Proteobacteria. The isolates constitute three new species with internal levels of DNA relatedness ranging from 44.9 to 51*3O/0.
    [Show full text]
  • Supplementary Information for Microbial Electrochemical Systems Outperform Fixed-Bed Biofilters for Cleaning-Up Urban Wastewater
    Electronic Supplementary Material (ESI) for Environmental Science: Water Research & Technology. This journal is © The Royal Society of Chemistry 2016 Supplementary information for Microbial Electrochemical Systems outperform fixed-bed biofilters for cleaning-up urban wastewater AUTHORS: Arantxa Aguirre-Sierraa, Tristano Bacchetti De Gregorisb, Antonio Berná, Juan José Salasc, Carlos Aragónc, Abraham Esteve-Núñezab* Fig.1S Total nitrogen (A), ammonia (B) and nitrate (C) influent and effluent average values of the coke and the gravel biofilters. Error bars represent 95% confidence interval. Fig. 2S Influent and effluent COD (A) and BOD5 (B) average values of the hybrid biofilter and the hybrid polarized biofilter. Error bars represent 95% confidence interval. Fig. 3S Redox potential measured in the coke and the gravel biofilters Fig. 4S Rarefaction curves calculated for each sample based on the OTU computations. Fig. 5S Correspondence analysis biplot of classes’ distribution from pyrosequencing analysis. Fig. 6S. Relative abundance of classes of the category ‘other’ at class level. Table 1S Influent pre-treated wastewater and effluents characteristics. Averages ± SD HRT (d) 4.0 3.4 1.7 0.8 0.5 Influent COD (mg L-1) 246 ± 114 330 ± 107 457 ± 92 318 ± 143 393 ± 101 -1 BOD5 (mg L ) 136 ± 86 235 ± 36 268 ± 81 176 ± 127 213 ± 112 TN (mg L-1) 45.0 ± 17.4 60.6 ± 7.5 57.7 ± 3.9 43.7 ± 16.5 54.8 ± 10.1 -1 NH4-N (mg L ) 32.7 ± 18.7 51.6 ± 6.5 49.0 ± 2.3 36.6 ± 15.9 47.0 ± 8.8 -1 NO3-N (mg L ) 2.3 ± 3.6 1.0 ± 1.6 0.8 ± 0.6 1.5 ± 2.0 0.9 ± 0.6 TP (mg
    [Show full text]
  • WO 2018/064165 A2 (.Pdf)
    (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2018/064165 A2 05 April 2018 (05.04.2018) W !P O PCT (51) International Patent Classification: Published: A61K 35/74 (20 15.0 1) C12N 1/21 (2006 .01) — without international search report and to be republished (21) International Application Number: upon receipt of that report (Rule 48.2(g)) PCT/US2017/053717 — with sequence listing part of description (Rule 5.2(a)) (22) International Filing Date: 27 September 2017 (27.09.2017) (25) Filing Language: English (26) Publication Langi English (30) Priority Data: 62/400,372 27 September 2016 (27.09.2016) US 62/508,885 19 May 2017 (19.05.2017) US 62/557,566 12 September 2017 (12.09.2017) US (71) Applicant: BOARD OF REGENTS, THE UNIVERSI¬ TY OF TEXAS SYSTEM [US/US]; 210 West 7th St., Austin, TX 78701 (US). (72) Inventors: WARGO, Jennifer; 1814 Bissonnet St., Hous ton, TX 77005 (US). GOPALAKRISHNAN, Vanch- eswaran; 7900 Cambridge, Apt. 10-lb, Houston, TX 77054 (US). (74) Agent: BYRD, Marshall, P.; Parker Highlander PLLC, 1120 S. Capital Of Texas Highway, Bldg. One, Suite 200, Austin, TX 78746 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
    [Show full text]
  • Microbial and Geochemical Investigation Down to 2000 M Deep Triassic Rock (Meuse/Haute Marne, France)
    geosciences Article Microbial and Geochemical Investigation down to 2000 m Deep Triassic Rock (Meuse/Haute Marne, France) Vanessa Leblanc 1,2,3, Jennifer Hellal 1 , Marie-Laure Fardeau 4,5, Saber Khelaifia 4,5, Claire Sergeant 2,3, Francis Garrido 1, Bernard Ollivier 4,5 and Catherine Joulian 1,* 1 BRGM, Geomicrobiology and Environmental Monitoring Unit, F-45060 Orléans CEDEX 02, France; [email protected] (V.L.); [email protected] (J.H.); [email protected] (F.G.) 2 Bordeaux University, Centre d’Etudes Nucleaires de Bordeaux Gradignan, UMR5797, F-33170 Gradignan, France; [email protected] 3 CNRS-IN2P3, Centre d’Etudes Nucleaires de Bordeaux Gradignan, UMR5797, F-33170 Gradignan, France 4 Aix Marseille Université, CNRS/INSU, IRD, Mediterranean Institute of Oceanography (MIO), UM 110, 13288 Marseille, France; [email protected] (M.-L.F.); Saber.Khelaifi[email protected] (S.K.); [email protected] (B.O.) 5 Université de Toulon, CNRS/INSU, 83957 La Garde, France * Correspondence: [email protected]; Tel.: +33-2-3864-3089 Received: 31 August 2018; Accepted: 13 December 2018; Published: 20 December 2018 Abstract: In 2008, as part of a feasibility study for radioactive waste disposal in deep geological formations, the French National Radioactive Waste Management Agency (ANDRA) drilled several boreholes in the transposition zone in order to define the potential variations in the properties of the Callovo–Oxfordian claystone formation. This consisted of a rare opportunity to investigate the deep continental biosphere that is still poorly known. Four rock cores, from 1709, 1804, 1865, and 1935 m below land surface, were collected from Lower and Middle Triassic formations in the Paris Basin (France) to investigate their microbial and geochemical composition.
    [Show full text]
  • Aquabacterium Limnoticum Sp. Nov., Isolated from a Freshwater Spring
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by National Chung Hsing University Institutional Repository International Journal of Systematic and Evolutionary Microbiology (2012), 62, 698–704 DOI 10.1099/ijs.0.030635-0 Aquabacterium limnoticum sp. nov., isolated from a freshwater spring Wen-Ming Chen,1 Nian-Tsz Cho,1 Shwu-Harn Yang,2 A. B. Arun,3 Chiu-Chung Young4 and Shih-Yi Sheu2 Correspondence 1Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine Shih-Yi Sheu University, no. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC [email protected] 2Department of Marine Biotechnology, National Kaohsiung Marine University, no. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC 3Yenepoya Research Center, Yenepoya University, University Rd, Deralakatee, Mangalore, Karnataka State, India 4College of Agriculture and Natural Resources, Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, ROC A Gram-negative, facultatively anaerobic, short-rod-shaped, non-motile and non-spore-forming bacterial strain, designated ABP-4T, was isolated from a freshwater spring in Taiwan and was characterized using the polyphasic taxonomy approach. Growth occurred at 20–40 6C (optimum, 30–37 6C), at pH 7.0–10.0 (optimum, pH 7.0–9.0) and with 0–3 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ABP-4T, together with Aquabacterium fontiphilum CS-6T (96.4 % sequence similarity), Aquabacterium commune B8T (96.1 %), Aquabacterium citratiphilum B4T (95.5 %) and Aquabacterium parvum B6T (94.7 %), formed a deep line within the order Burkholderiales.
    [Show full text]
  • Microbial and Mineralogical Characterizations of Soils Collected from the Deep Biosphere of the Former Homestake Gold Mine, South Dakota
    University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln US Department of Energy Publications U.S. Department of Energy 2010 Microbial and Mineralogical Characterizations of Soils Collected from the Deep Biosphere of the Former Homestake Gold Mine, South Dakota Gurdeep Rastogi South Dakota School of Mines and Technology Shariff Osman Lawrence Berkeley National Laboratory Ravi K. Kukkadapu Pacific Northwest National Laboratory, [email protected] Mark Engelhard Pacific Northwest National Laboratory Parag A. Vaishampayan California Institute of Technology See next page for additional authors Follow this and additional works at: https://digitalcommons.unl.edu/usdoepub Part of the Bioresource and Agricultural Engineering Commons Rastogi, Gurdeep; Osman, Shariff; Kukkadapu, Ravi K.; Engelhard, Mark; Vaishampayan, Parag A.; Andersen, Gary L.; and Sani, Rajesh K., "Microbial and Mineralogical Characterizations of Soils Collected from the Deep Biosphere of the Former Homestake Gold Mine, South Dakota" (2010). US Department of Energy Publications. 170. https://digitalcommons.unl.edu/usdoepub/170 This Article is brought to you for free and open access by the U.S. Department of Energy at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in US Department of Energy Publications by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. Authors Gurdeep Rastogi, Shariff Osman, Ravi K. Kukkadapu, Mark Engelhard, Parag A. Vaishampayan, Gary L. Andersen, and Rajesh K. Sani This article is available at DigitalCommons@University of Nebraska - Lincoln: https://digitalcommons.unl.edu/ usdoepub/170 Microb Ecol (2010) 60:539–550 DOI 10.1007/s00248-010-9657-y SOIL MICROBIOLOGY Microbial and Mineralogical Characterizations of Soils Collected from the Deep Biosphere of the Former Homestake Gold Mine, South Dakota Gurdeep Rastogi & Shariff Osman & Ravi Kukkadapu & Mark Engelhard & Parag A.
    [Show full text]
  • Bottled Aqua Incognita: Microbiota Assembly and Dissolved
    bioRxiv preprint doi: https://doi.org/10.1101/154732; this version posted June 23, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 Bottled aqua incognita: 2 Microbiota assembly and dissolved organic matter diversity 3 in natural mineral waters 4 5 Celine C. Lesaulnier1#, Craig W. Herbold1#, Claus Pelikan1, David Berry1, Cédric Gérard4, Xavier Le 6 Coz4, Sophie Gagnot4, Jutta Niggemann3, Thorsten Dittmar3, Gabriel A. Singer2#, and 7 Alexander Loy1* 8 9 1University of Vienna, Research Network Chemistry meets Microbiology, Department of Microbiology and 10 Ecosystem Science, Division of Microbial Ecology, Althanstrasse 14, A-1090 Vienna, Austria 11 2Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Department of Ecohydrology, 12 Müggelseedamm 310, D-12587 Berlin, Germany. 13 3University of Oldenburg, Institute for Chemistry and Biology of the Marine Environment, ICBM-MPI 14 Bridging Group for Marine Geochemistry, Carl-von-Ossietzky-Straße 9-11, D-26129 Oldenburg, Germany. 15 4Nestec Ltd., Route du Jorat 57, CH-1000 Lausanne 26, Switzerland 16 17 #Authors contributed equally to this study 18 *Corresponding author: Alexander Loy, University of Vienna, Research Network Chemistry meets 19 Microbiology, Department of Microbiology and Ecosystem Science, Division of Microbial Ecology, 20 Althanstrasse 14, A-1090 Vienna, Austria; [email protected]; Tel. +43 1 4277 76605 21 22 Short title: Microbiota and DOM diversity in bottled waters 23 24 Key words: bottled water, microbial diversity, dissolved organic matter, Fourier transform ion cyclotron 25 resonance mass spectrometry, Aquabacterium, Curvibacter, Polaromonas 26 27 28 29 30 31 32 33 34 35 1 bioRxiv preprint doi: https://doi.org/10.1101/154732; this version posted June 23, 2017.
    [Show full text]
  • Heavy Metal(Loid) Transformations by Bacteria Isolated from Extreme Environments
    Heavy Metal(loid) Transformations by Bacteria Isolated from Extreme Environments By Chris Maltman A Thesis Submitted to the Faculty of Graduate Studies in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy Department of Microbiology University of Manitoba Winnipeg, Manitoba Canada Copyright © 2016 by Chris Maltman I Abstract The research presented here studied bacteria from extreme environments possessing strong resistance to highly toxic oxyanions of Te, Se, and V. The impact of tellurite on cells of aerobic anoxygenic phototrophs and heterotrophs from freshwater and marine habitats was 2- investigated. Physiological responses of cells to TeO3 varied. In its presence, biomass either increased, remained similar or decreased, with ATP production following the same trend. Four detoxification strategies were observed: 1) Periplasmic based reduction; 2) Reduction needing an intact cytoplasmic membrane; 3) Reduction involving an undisturbed whole cell; and 4) Membrane associated reduction. The first three require de novo protein synthesis, while the last was constitutively expressed. We also investigated two enzymes responsible for tellurite reduction. The first came from the periplasm of deep-ocean hydrothermal vent strain ER-Te-48 associated with tube worms. The second was a membrane associated reductase from Erythromonas ursincola, KR99. Both could also use tellurate as a substrate. ER-Te-48 also has a second periplasmic enzyme which reduced selenite. Additionally, we set out to find new organisms with the ability to resist and reduce Te, Se, and V oxyanions, as well as use them for anaerobic respiration. New strain CM-3, a Gram negative, rod shaped bacterium from gold mine tailings of the Central Mine in Nopiming Provincial Park, Canada, has very high level resistance and the capability to perform dissimilatory anaerobic reduction of tellurite, tellurate, and selenite.
    [Show full text]
  • In Situ Examination of Microbial Populations in a Model Drinking
    In situ examination of microbial populations in a model Water Science and Technology: Supply drinking water distribution system A.C. Martiny*,**, A.T. Nielsen***, E. Arvin**, S. Molin* and H.-J. Albrechtsen** * BioCentrum-DTU, Technical University of Denmark, 2800 Lyngloy, Denmark ** Environment and Resources, Technical University of Denmark, 2800 Lyngby, Denmark *** Exiqon A/S, 2950 Vedbaek, Denmark Abstract A flow cell set-up was used as a model drinking water distribution system to analyze the in situ microbial population. Biofilm growth was followed by transmission light microscopy for 81 days and showed a biofilm consisting of microcolonies separated by a monolayer of cells. Protozoans (ciliates and flagellates) were often seen attached to the microcolonies. The biofilm was hybridized with oligonucleotide probes specific for all bacteria and the α- and β-subclass of Proteobacteria and visualized with a scanning confocal laser microscope. Hybridization showed that the microcolonies primarily consisted of a mixed population of Vol 2 No 3 pp 283–288 α β - and -Proteobacteria. 65 strains from the inlet water and 20 from the biofilm were isolated on R2A agar plates and sorted into groups with amplified rDNA restriction analysis. The 16S rDNA gene was sequenced for representatives of the abundant groups. A phylogenetic analysis revealed that the majority of the isolated strains from the bulk water and biofilm were affiliated to the family of Comamonadaceae in the β-lineage of Proteobacteria. The majority of the strains from the α-lineage were affiliated to the family of Sphingomonadaceae. We were unable to detect any strains from the Pseudomonas genus and found a low γ abundance of bacteria affiliated to the -subclass of Proteobacteria where Pseudomonas and E.
    [Show full text]