Natural Sciences Research Institute UP Diliman, Quezon City ANNUAL REPORT 2018 A YEAR OF ACCOMPLISHMENTS
University of the Philippines Diliman, Quezon City
TABLE OF CONTENTS
PART I. Introduction
Vision, Mission ii Message of the Director iii Brief History and Program Goals iv
PART II. Programs and Other Activities
1. Research Programs and Publications 1
1.1 NSRI-funded Researches 1
List of Terminated Projects in 2018 1 List of Extended Projects in 2018 3 List of Approved Projects for Funding in 2018 7 List of Commitment Projects in 2018 18 List of Externally-Funded Researches in 2018 21
1.2 Publication and Creative Works 39
2. Extension Services 43
2.1 Technical Assistance and Advisory Services 43
Biological Research and Services Laboratory (BRSL) 44 DNA Analysis Laboratory (DAL) 61 Microbiological Research and Services Laboratory (MRSL) 80 Research and Analytical Services Laboratory (RASL) 96
2.2 Information Service 126 2.3 Community Service 126 2.4 Biosafety Program 127 2.5 Lecture Series 127
PART III. Resources
3. Organization and Personnel 130
Organization and Management 130
Executive Council 130 Research Section 131 Technical Support Section 132 Administrative Support Section 133 List of NSRI Room Occupants 2018 134 Personnel Inventory of NSRI 135
3.1 NSRI Staff Development in 2018 138 3.2 Resource Generation and Allocations 142 3.3 Fiscal Resources 142
PART IV. Conclusion
Future Plans and Recommendations 145
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NATURAL SCIENCES RESEARCH INSTITUTE
Vision
A world-renowned National Center of Excellence for research and technical services in the disciplines and multi-disciplines of biology, chemistry, environmental, mathematical, and atmospheric sciences.
Mission
• To provide opportunities for the development of high caliber scientific and technological human resources. • To attain world-class status for its research and scientific contributions. • To attain world-class status for its various technical service laboratories. • To take the initiative in the advocacy for the protection of the environment. • To take the leadership in the advancement and application of science and technology for the improvement of the quality of life of the Filipino.
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MESSAGE OF THE DIRECTOR
The Natural Sciences Research Institute (NSRI) has had another strong year. It continues to lead the way and build on past strengths. I am proud to see first-hand how the Institute’s research advances are benefiting society and improving lives. With members producing high quality publications in 2018, many research discoveries are emerging.
I am pleased not only by the Institute’s research and extension works but also the willingness of members to share their findings with our community through media, seminars and scientific conferences. This communication is necessary to raise awareness, influence policy and practice, and educate people. I am confident in the ability of the NSRI to make significant improvements for the lives of generations to come.
As always, I am grateful for the University’s continued encouragement and support in the value of the Institute’s research and its significance for the University’s impact.
I am looking forward for another exciting year ahead.
Mabuhay ang NSRI !
WINDELL L. RIVERA, Ph.D. Director
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BRIEF HISTORY AND PROGRAM GOALS
June 19, 1964 Republic Act 3887, signed by President Diosdado Macapagal, for the establishment of the Natural Sciences Research Center (NSRC) as amended by Republic Act 6069 (1969).
February 1965 U.P. President Carlos P. Romulo organized the Executive Advisory Council with the task of getting funds for the Center.
July 1971 NSRC was first opened to researchers.
November 13, 1972 Formal inauguration and dedication ceremonies of the UP-NSRC by U.P. President Salvador P. Lopez.
March 23, 1983 Executive Order No. 889, issued by President Ferdinand E. Marcos, organized the Natural Sciences Research Institute (NSRI) from the Natural Sciences Research Center (NSRC).
May 26, 1983 Approval of the Implementing Guidelines for the creation of the NSRI out of NSRC by the UP Board of Regents.
July 2, 1985 Executive Order No. 7, signed by UP President Edgardo J. Angara, provided for the organizational structure and staffing pattern of the NSRI.
December 18, 1986 Executive Order No. 6, signed by UP President Edgardo J. Angara, amended EO No. 7 to include interdisciplinary programs.
February 28, 2000 The NSRI building was named Miranda Hall in honor of the late Prof. Bienvenido T. Miranda, the Institute's first Director.
The NSRI serves as the national center of excellence for the advancement, dissemi- nation, and application of knowledge in Biology, Chemistry, Environmental and Atmospheric Sciences and Mathematics. It is mandated to:
a. undertake research in Biology, Chemistry, Environmental and Atmospheric Sciences, Mathematics, and organize interdisciplinary research programs.
b. support the graduate programs of the College of Science in these fields of study.
c. provide extension and other services on request and when feasible do research needs such as consultancies, research, training, technical support, analytical support to researchers, other units of the University, and other institutions and agencies, both government and private.
d. disseminate research findings through scientific publications, workshops, conferences and seminars.
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1. RESEARCH PROGRAMS AND PUBLICATIONS
1.1 NSRI-FUNDED RESEARCHES
RESEARCH SUPPORT: MOOE, FACILITIES, HONORARIA AND SALARIES OF PROJECT PERSONNEL
LIST OF TERMINATED PROJECTS IN 2018
BIOLOGY
1) Project title : Analysis of Virulence-Associated Genes of Leptospira Isolated from Environmental Samples in the Philippines
Project code : BIO-17-1-09 Funding source : NSRI Amount of funding : 2017 – P 620,401.00 Duration of project : January 1, 2017 – December 31, 2017 Project leader : Dr. Windell L. Rivera Personnel : 1 University Research Associate I
Title of Publication : Identification of Leptospira spp. from Environmental Sources in Areas with High Human Leptospirosis Incidence in the Philippines
Abstract:
Leptospira is the causative agent of leptospirosis, which is considered an emerging major threat to public health due to its increasing frequency reported worldwide. In the Philippines, the prevalence of the disease continuously rises, particularly in urban areas. Because leptospirosis is commonly transmitted through contact with contaminated environment, water and soil samples were collected in regions in the Philippines where high incidence of human leptospirosis cases was reported recently. Of the 54 samples screened for the presence of Leptospira, 35% were found positive through 23S rRNA gene PCR-based detection. None were found positive when primers targeting lipL32, lipL41, and ompL1 genes were used. Most of these isolates were collected from rural areas. However, 165 rRNA gene sequencing identified all isolates to be L. yanagawae and L. meyeri, which are nonpathogenic. Despite the lack of evidence of the presence of pathogenic species in the environmental sources, the results still suggest that leptospires persist in these areas. These data are crucial for environmental monitoring and identification of contaminated areas where humans may be at risk of infection.
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CHEMISTRY
2) Project title : Polymeric Nanocarriers Formed by Hyperbranched Copolymers of Poly(ethylene glycol) and Methacrylic Acid as Drug Delivery System
Project code : CHE-17-1-08 Funding source : NSRI Amount of funding : 2017 – P 570,001.00 Duration of project : January 1, 2017 – December 31, 2017 Project leader : Dr. Susan D. Arco Personnel : 1 University Research Associate I
Title of Publication : Temperature- and pH-Dependent Drug Release of Block Copolymers of Methacrylic Acid and Poly(Ethylene Glycol) Methyl Ether Methacrylates
Abstract:
The block copolyrners, poly(methacrylic acid)-b-poly(di(ethylene glycol) methyl ether methacrylate) (Block-D) and poly(methacrylic acid)-b-poty(polyethyIene glycol) methyl ether methacrylate) (Block-P) were synthesized via reversible addition- fragmentation chain transfer (RAFT) polymerization towards the development of stimuli-responsive carriers of poorly water-soluble drugs. The structures of the copolymers and the confirmation of successful block copolymerization were studied using proton nuclear magnetic resonance (1H-NMR) spectrosropy. Data from gel permeation chromatography (GPC) then showed polydispersity indices (PDI) close to 1.0, characteristic of RAFT polymerization. Stimuli-response studies revealed Block-D was responsive at pH 5.15 and 26oC while Block-P was responsive at pH 5.15 and 72oC. The corresponding micelles had particle sizes of 92.95-201.4 nm, as determined by dynamic light scattering (DLS), with critical micelle concentration (CMC) at about 10-1 mg/mL, per fluorescence studies. Using ibuprofen as the model drug, the drug loading content reached 11.76%, at 66.65% efficiency. In vitro release profiles then demonstrated 18% drug release within 5 h at stomach-like conditions and 65% release within 5 h at small intestine-Iike conditions. Ultimately, cell viability assays of the blank and loaded micelles confirmed that neither is cytotoxic. These results show the immense potential and capability of the synthesized material as a drug delivery system for poorly water-insoluble drugs
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LIST OF EXTENDED PROJECTS IN 2018
BIOLOGY
1) Project title : Emerging Leptospirosis and Toxoplasmosis in Marine Wildlife Reservoir Hosts: The Case of Cetaceans Stranded in Philippine Waters
Project code : BIO-17-1-02 (6 months extension) Funding source : NSRI Amount of funding : 2017 – P 620,401.00 (No additional funds for 2018) Duration of project : January 1, 2017 – December 31, 2017 Project leader : Dr. Marie Christine M. Obusan Co-Project leader : Dr. Windell L. Rivera Collaborators : Dr. Lemnuel V. Aragones Dr. Maria Auxilia T. Siringan Personnel : 1 University Research Associate I
Objectives of the project:
The proposed project is part of the research agenda “Philippines as an emerging disease hotspot: surveillance in local wildlife reservoir hosts”. Monitoring the prevalence of emerging infectious diseases and their pathogens through wildlife is a practical approach in a developing country with limited resources for purposive clinical diagnoses, prevention, and treatment. Such effort is deemed as a contribution to the worldwide One Health initiative.
The project generally aims to detect the occurrence of Ieptospirosis and toxoplasmosis in cetaceans found in the Philippines. Specifically, it aims to:
1. Screen stranded cetaceans for Leptospira spp. and Toxoplasma gondii in target tissues; 2. Confirm acute or chronic Ieptospirosis or toxoplasmosis infection based on microbiological, molecular, and gross pathological criteria; 3. Identify risk factors associated with Ieptospirosis and toxoplasmosis infection and transmission among cetaceans.
The following scientific problems are intended to be solved: 1. Are cetaceans found in the Philippines reservoir hosts for Leptospira spp. and T. gondii? 2. Are toxoplasmosis and Ieptospirosis emergent in local cetacean populations? 3. What are the risk factors associated for the occurrence of Leptospira spp. and T. gondii in cetaceans? 4. What are the risk factors for zoonotic transmission of Leptospira spp. and T. gondii from local cetaceans?
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2) Project title : Assessment of Laminarinase Enzymes from Various Sources for the Control of Alternaria solani, a Phytopathogenic Fungi of Tomato, Eggplant and Pepper
Project code : BIO-17-1-03 (6 months extension) Funding source : NSRI Amount of funding : 2017 – P 446,801.00 (No additional funds for 2018) Duration of project : January 1, 2017 – December 31, 2017 Project leader : Dr. Jessica F. Simbahan Co-Project leader : Dr. Virgie A. Alcantara Personnel : 1 University Research Associate I Objectives of the project:
The major objective of the project is to identify major sources of laminarinase enzymes and determine the ability of the enzyme(s) to control Alternaria solani, causative agent for leaf blight in tomato, eggplant and pepper.
The specific objectives of the project are to: 1. Identify major sources of laminarinase by screening isolates from samples of mangrove detritus, soil and other sources, 2. Quantify laminarinase activity of the potential isolates, 3. Determine antagonistic activity of laminannase against Altemaria solani, 4. Purify laminarinase by ammonium sulfate precipitation and column chromatography, and 5. Partially characterize potential laminarinase(s) in terms of optimum pH, temperature and molecular weight.
Early blight is a major fungal disease of tomatoes, eggplant, pepper and potatoes caused by the fungus, Altemaria solani. lt is very common in the tropics especially under warm weather and very humid or wet conditions. The disease affects the older lower leaves and stems of the plants and eventually affects the fruits. It is estimated that global expenditure for the control of A. solani is around $77M annually.
Fungal cell walls are made up of chitin, 1,3-B-glucan or laminarin, other polymers and proteins. Laminarin accounts for more than 60% of the cell wall. Chitin, on the other hand, accounts for 10-20% of the cell wall of filamentous fungi. Laminarin and chitin are hydrolyzed by 1,3-B-glucanases or laminarinases and chitinases, respectively.
This proposal aims to identify major laminarinase producers and test the antagonistic effect of crude and purified enzyme preparations against A. solani, a major phytopathological fungi. Because a large percentage of the fungal cell wall is made up of laminarin, this proposal aims to prove that laminarinase will be an effective agent that can kill the fungi.
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The expected output from this project will be the identification of at least two major laminarinase producers, purification of their enzymes and their partial characterization. The antagonistic effect of the enzymes on A. solani will also be demonstrated using plate assay. These initial results will contribute to the development of a concoction of enzymes which will be formulated into a biofungicide against major phytopathogenic fungi of commercial importance.
3) Project title : Plant Species Diversity in UP Diliman and Vicinity: UP Arboretum
Project code : BIO-17-1-04 (6 months extension) Funding source : NSRI Amount of funding : 2017 – P 410,361.48 (No additional funds for 2018) Duration of project : January 1, 2017 – December 31, 2017 Project leader : Dr. Joel DLC. Siopongco Collaborator : Dr. Annalee S. Hadsall Personnel : 1 University Research Associate I
Objectives of the project:
1. Classify existing plant species in UP Diliman campus including the UP Arboretum. 2. Include survey of lower vascular plants: pteridophytes (ferns and fern allies) and bryophytes (mosses). 3. Collect herbarium specimens for archival and learning purposes. 4. Plant listing will serve as reference point for any future endeavors if and when the present ecosystem will be disturbed. 5. Establishment of a field bank (gene bank), in-situ, i.e., the conservation of species in their natural habitats, thus conserving biodiversity. 6. Recommend ex-situ gene bank, i.e. conservation/preservation of components of biological diversity outside their natural habitats, especially for displaced species.
ENVIRONMENTAL SCIENCE AND METEOROLOGY
4) Project title : Spatiotemporal Distribution and Relative Abundance of Small Cetaceans Along Southern Tañon Strait in Relation to Environmental Factors
Project code : ESM-17-1-01 (6 months extension) Funding source : NSRI Amount of funding : 2017 – P 614,401.00 (No additional funds for 2018) Duration of project : January 1, 2017 – December 31, 2017 Project leader : Dr. Olivia C. Cabrera Collaborator : Dr. Lemnuel V. Aragones Personnel : 1 University Research Associate l
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Objectives of the project:
The overall objective of this study is to analyze, integrate and predict the spatiotemporal distribution and relative abundance of small cetaceans along southern Tañon Strait in relation to environmental factors. Spatiotemporal distribution and relative abundance of cetaceans in relation to environmental parameters have yet to be studied comprehensively. Thus, this study aims to contribute significant knowledge to Philippine marine mammal science and establish management and conservation strategies.
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LIST OF APPROVED PROJECTS IN 2018
BIOLOGY
1) Project title : Population Genetic analysis and DNA Barcoding of Caranx sp. in Batangas
Project code : BIO-18-1-01 Funding source : NSRI Amount of funding : 2018 – P 619,129.00 Duration of project : January 1, 2018 – December 31, 2018 Project leader : Dr. Brian S. Santos Personnel : 1 University Research Associate I
Objectives of the project:
The general objective of this study is to perform genetic diversity analysis and DNA barcoding for Caranx species.
The specific objectives are as follows:
1) Molecular identification of C. ignobilis and C. sexfasciatus, including their marine and freshwater forms, based on cytochrome c. oxidase I gene (COl) sequences
This objective will answer the problem of misidentification associated in the two species, which was observed in a pilot study and through analysis of GenBank sequences, Misidentification was also documented in Willette and Padin (2014). This will be supplemented with morphological data based on traditional morphometrics and geometric morphometrics
2) Assess the genetic diversity of C. ignobilis populations using mitochondrial control region,
This will provide baseline information of the genetic profile of C. ignobilis, which may soon emerge as an important aquaculture commodity. Population structure revealing the presence of subpopulations within the region may or may not be detected. Genetic diversity will be characterized as low or high in relation to other related taxa,
2) Project title : Population Genetic Structuring of a Widespread Tropical Fig Tree Ficus septica
Project code : BIO-18-1-02 Funding source : NSRI Amount of funding : 2018 – P 619,129.00 Duration of project : January 1, 2018 – December 31, 2018
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Project leader : Dr. Lillian Jennifer V. Rodriguez Personnel : 1 University Research Associate I
Objectives of the project:
With this project, we will aim to describe the spatial genetic structuring of Ficus septico (hauili) trees in the Philippines and to delimit the boundaries of the different F. septica populations.
The major result of this project is a map or graphic of the different genetic clusters of F. septica tree populations in the Philippines. The results of this study will be written up as a scientific manuscript that will be submitted in an internationally peer- reviewed scientific journal and as a laymanized article for dissemination to the public (i.e. DENR, restoration practitioners, nursery managers and stakeholders) through popular media.
3) Project title : Molecualar Detection and Identification of Leptospira spp. Isolated from Pediatric and Adult Patients in the Philippines
Project code : BIO-18-1-03 Funding source : NSRI Amount of funding : 2018 – P 663,129.00 Duration of project : January 1, 2018 – December 31, 2018 Project leader : Dr. Windell L. Rivera Personnel : 1 University Research Associate I
Objectives of the project:
Leptospira, the causative agent of leptospirosis, is considered a major threat to our country's public health due to the increasing incidence reported not only in Metro Manila but in other regions as well. Leptospirosis is most prevalent during rainy season where flooding mostly occur. As previously described, clinical presentation of the disease varies among pediatric and adult patients which may be due to the differences in the infecting strain. Thus, investigating the species of Leptospira that can be isolated from Filipino patients is deemed necessary in order to identify the circulating species present in our country. The present study will focus on detecting and classifying the Leptospira spp. that can be isolated from Filipino pediatric and adult patients through DNA sequencing and describing the disease spectrum that can be observed between the two age groups.
4) Project title : Prevalence of Virulence Genes and Multilocus Sequence Typing Genotypes in Campylobacter jejuni from Chickens in Metro Manila
Project code : BIO-18-1-04 Funding source : NSRI
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Amount of funding : 2018 – P 619,129.00 Duration of project : January 1, 2018 – December 31, 2018 Project leader : Dr. Gil M. Penuliar Personnel : 1 University Research Associate I
Objectives of the project:
The World Health Organization (WHO, 2017) lists Campylobacter as one of the four key global causes of diarrheal diseases. It is one of the most important enteropathogens in developing and industrialized countries, with the number of cases surpassing those of salmonellosis and shigellosis. Campylobacter jejuni a causative agent of campylobacteriosis, and is one of the common causes of bacterial enteritis. It is said to account for around 90% of human cases. It inhabits the gastrointestinal tract of domestic animals, and transmission to humans can occur through several routes. Infections are often caused by consumption of undercooked or cross-contaminated foods, particularly, poultry products. Epidemiological studies have shown that C. jejuni can persist in chicken houses or be introduced by contaminated equipment. ln Metro Manila, we have previously reported that 64% of sampled chicken products were contaminated with C. jejuni, which represents a significant health risk to consumers.
Campylobacter jejuni is naturally transformable, and this property majorly impacts the population structure and evolution of the species. There have been reports of genetic differentiation between farmed ruminants and chickens and between wild birds and farmed chickens within the same farm. The extent of difference in Campylobacter populations among hosts and environmental niches and the significance of temporal and geographical effects are not completely understood. In the Philippines, such information is currently unavailable. The heterogeneity of C. jejuni has made studying the pathogenicity of this pathogen particularly challenging. Genotyping methods have shown that C. jejunis genetically diverse and analyses of different isolates generally show significant strain variety in virulence gene and genotype profiles. Currently, PCR amplification of virulence genes and multilocus sequence typing (MLST) are common techniques used for the epidemiological and population genetic studies of many microorganisms like C. jejuni. This can address existing gaps in the understanding of C. jejuni ecology, pathogenesis, and epidemiology, which can provide important insights into the origins of infection and how it is spread.
5) Project title : Establishment of Pre-inductive Culture Conditions for the Endodermal Differentiation of Human Induced Pluripotent Stem Cells
Project code : BIO-18-1-05 Funding source : NSRI Amount of funding : 2018 – P 220,000.00 Duration of project : January 1, 2018 – December 31, 2018 Project leader : Dr. Ahmad Reza F. Mazahery
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Objectives of the project:
Over the recent years, there has been a rapid increase in published work on iPSCs. Being arguably the most important breakthrough in regenerative medicine to date, iPSCs can eliminate the need for the use of embryonic stem cells, which causes ethical controversies. More importantly it promises to be a more efficient therapeutic remedy towards many histopathological diseases such as, pancreatitis, diabetes, liver cancer, Alzheimer’s disease and many more.
There are some critical issues in generating iPSC-derived tissues. Among them is that iPSC-derived tissues need to competently express all the expected genetic and physiological markers for differentiation. Also, the issue of developing a strategy that will enable large-scale generation of such tissues for either research or medical applications must be addressed.
Aside from its prospect in medical applications, iPSC-derived tissues can also be used for construction of in vivo and in vitro models to study diseases. For example, hepatic-like cells generated from iPSCs that express hepatitis receptors can be used to study the mechanism of hepatitis virus interaction with the cell membrane. Alternately, hepatic-like cells can also be transplanted into appropriate transgenic mice in order to create mice models that have humanized liver. Such mice can be used to better understand the molecular genetics and pathology of several rare liver diseases.
This project is expected to have two main benefits. First, it will provide local researchers in regenerative medicine access to pluripotent stem cell lines. In recent years, many stem cell institutes have been established in the Philippines. However, these institutes have mainly used adult stem cells, which have very limited potency and replicative capability. To the best of the applicant's knowledge, no research group in the Philippines is currently working on iPSCs. The second main benefit is that it will help establish future strategies for inducing iPSCs to differentiate towards mature endoderm-lineaged tissues like the liver or the pancreas.
6) Project title : Isolation, Identification and Screening of Citric Acid Producing Fungal Endophytes from Citrus microcarpa
Project code : BIO-18-1-06 Funding source : NSRI Amount of funding : 2018 – P 669,129.00 Duration of project : January 1, 2018 – December 31, 2018 Project leader : Dr. Pierangeli G. Vital Personnel : 1 University Research Associate I
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Objectives of the project:
The biosynthesis of citric acid product from citric acid-producing fungi is an alternative and safer means as compared to synthetic chemistry methods or processes. The study would not be using expensive reagents as fungal substrates to minimize production. Currently, the methods employed include microbial fermentation, synthetic chemistry, and citrus fruit extraction. However, synthetic methods are costly and produce toxic wastes, while citrus fruit extraction is time consuming and tedious as plants undergo perennial life cycle. On the other hand, microbial fermentation is environment al friendly and sustainable. This project will be highly beneficial to farmers, consumers, vendors, and industrial companies towards improved product quality and food safety.
CHEMISTRY
7) Project title : Synthesis and Characterization of Urea-Based Molecules as Hydro- and Organogelators
Project code : CHE-18-1-01 Funding source : NSRI Amount of funding : 2018 – P 419,131.00 Duration of project : January 1, 2018 – December 31, 2018 Project leader : Dr. Monissa C. Paderes Personnel : 1 University Research Associate I
Objectives of the project:
The goal of this project is to synthesize and characterize a series of low molecular weight molecules, which contain urea functionalities that could be potential hydro- and organogelators. Full characterization of the synthesized compounds will be performed via 1H and 13C NMR spectroscopy, MS and FT-IR analysis. These compounds will be screened as molecular gelators whether hydro- or organogelators using the standard gelation technique. Water and other commonly used organic solvents will be utilized for the gelation experiments. Characterization of the gels formed will be performed using electron microscopy such as SEM and AFM.
8) Project title : Exploration of the Regioselective Acylation of Monosaccharides in Ionic Liquid
Project code : CHE-18-1-02 Funding source : NSRI Amount of funding : 2018 – P 725,129.00 Duration of project : January 1, 2018 – December 31, 2018 Project leader : Dr. Medel Manuel L. Zulueta Collaborator : Dr. Susan D. Arco Personnel : 1 University Research Associate I
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Objectives of the project:
Regioselective acylation is an important aspect of functional group modification and oligosaccharide assembly in carbohydrate synthesis. This proposed study aims to examine the possibility of regioselective acylation in ionic liquids as a greener alternative to conventional procedures. The following are the specific objectives of the proposed study. It is anticipated that regioselectivity could be achieved and could be applied in practice as part of the usual carborydrate synthetic schemes.
1) To prepare 1-butyl-3-imidazolium benzoate as among the ionic liquids that will be tested for regioselective acylation. 2) To prepare several diols and triols based on common monsaccharide backbones, such as glucose, glucosamine, mannose and galactose. 3) To evaluate the regioselectivity of acetylation and benzoylation in a selected set of ionic liquids with variation in counteranions, temperature, reaction time and addition of acid or base catalysts. 4) Thoroughly characterize the products by IR, NMR and Mass spectroscopy
9) Project title : Peptide-based Metal-Organic Frameworks (Peptide- MOFs) for Chiral Resolution of Racemic Drugs
Project code : CHE-18-1-03 Funding source : NSRI Amount of funding : 2018 – P 624,929.00 Duration of project : January 1, 2018 – December 31, 2018 Project leader : Dr. Marlon T. Conato Personnel : 1 University Research Associate I
Objectives of the project:
The primary objective of the project is to synthesize new framework materials based on transition metal ions coordinated with chiral peptides. The products will then be used for enantiomeric selective separation of model chiral compounds. In this project, racemic compounds such as butanol, benzoin, and ibuprofen are the target molecules tor separation based on their structural variation and size compatibility with peptide-MOFs architectures. Discovery of new chiral reagents that could separate enantiomers rapidly would immensely help companies such as the pharmaceutical industry deliver better quality products.
The specific objectives include:
1. Synthesis of new MOFs based on Ni2+, Co2+, and Zn2+ and small peptides like Gly-L- His-Gly, and other tripeptides and tetrapeptides with chird amino acid in the sequence; 2. Characterization and structural analysis of the products by lR, XRD, CD-ORD, and TGA;
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3. Enantiomeric separation of opticdly-active molecules like butanol, benzoin, and ibuprofen for potential use in chiral separation and purification of drugs and organic compounds.
10) Project title : Atomistic Stimulation of the Nucleation of Atmospherically Relevant Four-Component Systems
Project code : CHE-18-1-04 Funding source : NSRI Amount of funding : 2018 – P 625,129.00 Duration of project : January 1, 2018 – December 31, 2018 Project leader : Dr. Ricky B. Nellas Personnel : 1 University Research Associate I
Objectives of the project:
The specific objectives of this study are:
1) To run Monte-Carlo simulations until convergence for water/n-nonane/1- butanol/urea and carbon dioxide/water/methane/argon; 2) To generate free energy plots, composition plots, and derive the average critical cluster composition of these mixtures; 3) To propose mechanisms and pathways for the formation of these mixtures.
11) Project title : SFG Studies of Phospholipid Monolayers
Project code : CHE-18-1-05 Funding source : NSRI Amount of funding : 2018 – P 624,256.00 Duration of project : January 1, 2018 – December 31, 2018 Project leader : Dr. Imee Su Martinez Personnel : 1 University Research Associate I
Objectives of the project:
The objective of this study is to use sum frequency generation spectroscopy (SFG) to specifically determine the collapse pressure of phospholipid monolayers POPC/POPG on glass. This will ensure that the condensed films created through Langmuir-Blodgett preparation are stable, and are in their most ideal compact state. This is particularly important in POPC/POPG monolayers, which are called fluidic phosopholipids where the liquid crystalline phase is difficult to identify due to the absence of the co-existence phase.
12) Project title : Aqueous Phase Study on Selected Metal Oxides Aged by Low Molecular Weight Organic Acids
Project code : CHE-18-1-06 Funding source : NSRI
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Amount of funding : 2018 – P 625,129.00 Duration of project : January 1, 2018 – December 31, 2018 Project leader : Dr. Rheo B. Lamorena-Lim Personnel : 1 University Research Associate I
Objectives of the project:
1) To study solution phase behavior of metal oxides aged via organic acid processing
a. To measure and compare the hydrodynamic radii of unprocessed and processed metal oxides b. To measure and compare the zeta potential unprocessed and processed metal oxides c. To obtain extinction spectra (sedimentation plots) of unprocessed and processed metal oxides
2) To determine the effects of organic acids on metal oxide dissolution
a. To compare pH of metal oxide suspensions processed by different low MW organic acid b. To perform dissolution experiments on unprocessed and processed metal oxides
13) Project title : Fabrication of Photocatalytic and Antimicrobial ZnO/Polymer Nanocomposite Thin Films
Project code : CHE-18-1-07 Funding source : NSRI Amount of funding : 2018 – P 631,129.00 Duration of project : January 1, 2018 – December 31, 2018 Project leader : Dr. Leon M. Payawan, Jr. Personnel : 1 University Research Associate I
Objectives of the project:
The general objective of this proposal is to develop a non-toxic and biocompatible polymer modified-zinc oxide nanoparticle-based coatings that has both potential anti-microbial and photocatalytic properties.
The specific objective aims to:
1) Synthesize zinc oxide nanoparticles via co-precipitation. This will be done with and without the use of common polymer stabilizers such as poly(ethylene glycol), poly(vinylpyrrolidone) and poly(acrylic acid). 2) Characterize the structure, size, physical morphology and spectroscopic properties of the synthesized zinc oxide nanoparticles 3) Assess its photocatalytic, antimicrobial and cytotoxic property.
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4) Fabricate thin films via layer-by-layer assembly of the nanoparticles with a known counter-electrolyte, poly(diallydimethylammonium chloride) unto various glass substrates (glass, indium tin oxide-coated/ITO glass slide).
14) Project title : Copolymerization of PEG-based Methacrylates with Methacrylic Acid in 1-alkyl-3 Methylimidazolium Hexafluorophosphate
Project code : CHE-18-1-08 Funding source : NSRI Amount of funding : 2018 – P 631,129.00 Duration of project : January 1, 2018 – December 31, 2018 Project leader : Dr. Susan D. Arco Personnel : 1 University Research Associate I
Objectives of the project:
1) Synthesize and characterize imidazolium-based ionic liquids with varying alkyl chain lengths. 2) Synthesize and characterize polymers of DEGMEMA and PEGMEMA via RAFT polymerization in room temperature ionic liquids (RTILs) and acetonitrile. 3) Copolymerize MAA with the synthesized P(DEGMEMA) and P(PEGMEMA) by RAFT polymerization in RTILs. 4) Determine the stimuli-responsive properties of the synthesized polymers by DLS and UV-Vis spectrophotometry. 5) Evaluate the biocompatibility of the synthesized polymers. 6) Compare the efficiency and rate of reaction of the polymerizations in RTILs and acetonitrile.
MATHEMATICS
15) Project title : Symplectic Completion of Matrices
Project code : MAT-18-1-01 Funding source : NSRI Amount of funding : 2018 – P 138,000.00 Duration of project : January 1, 2018 – December 31, 2018 Project leader : Dr. Jonathan V. Caalim Co-Project leader : Dr. Ralph John L. Dela Cruz
Objectives of the project:
This project is built upon the Institute of Mathematics’ excellent research contribution on matrix theory. The Institute has produced quality papers on a number of topics in this field, with publications in top journals like the Linear Algebra and Applications (LAA) Journal. In relation to symplectic matrices, colleagues from the
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Institute have considered various aspects of these objects - including the problem of decomposing symplectic matrices into symplectic matrices with additional properties. Our focus is the symplectic completion problem on matrices. As far as the proponents know, there is no complete criteria that discriminate whether a given matrix can be completed symplectically or not. In general, such mathematical problems of classification are difficult. As the objects we are considering are theoretic in nature, our contribution, in the form of theorems and propositions, will reside on the theoretical side. The expected output is a paper published, at the minimum locally. In the course of the research, it is hoped that certain computations will lead to more open problems which we can tackle in a future research project.
16) Project title : Local Trivialization of Bundles of Algebras
Project code : MAT-18-1-02 Funding source : NSRI Amount of funding : 2018 – P 119,030.00 Duration of project : January 1, 2018 – December 31, 2018 Project leader : Dr. Clarisson Rizzie P. Canlubo
Objectives of the project:
The objectives of the projects are as follows:
1) To give necessary and sufficient conditions for weak algebra bundles to be strict algebra bundles, 2) Study weak algebra bundles, more specifically, explore its relations with known and established objects in math.
17) Project title : Sign Patterns that Require or Allow Algebraic Positivity
Project code : MAT-18-1-03 Funding source : NSRI Amount of funding : 2018 – P 144,600.00 Duration of project : January 1, 2018 – December 31, 2018 Project leader : Dr. Diane Christine P. Pelejo
Objectives of the project:
This research project aims to identify sign pattern matrices that either allow or require algebraic positivity. It is also the goal of the proponent to publish an article in the Matimyas Matematika journal within one year. The proponent plans to involve an undergraduate student, Mr. Jean Abagat, in the research project. Mr. Abagat is a 4th year B.S. Mathematics student in the University of the Philippines Diliman who has expressed interest in doing research on the subject for his undergraduate thesis.
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18) Project title : Symmetric Perfect Colorings of Groups
Project code : MAT-18-1-04 Funding source : NSRI Amount of funding : 2018 – P 230,800.00 Duration of project : January 1, 2018 – December 31, 2018 Project leader : Dr. Rovin B. Santos Co-Project leader(s) : Dr. Lilibeth D. Valdez Dr. Ma. Lailani B. Walo
Objectives of the project:
This research project aims to contribute in the study of symmetric colorings by relating it to the concept of perfect colorings. In particular, we consider coset colorings for certain groups and derive conditions under which the coset coloring is symmetric. We provide some examples of symmetric perfect colorings of cyclic and dihedral groups, and symmetric groups of platonic solids. We also extend the results to some infinite groups such as the frieze groups and plane crystallographic groups.
19) Project title : The Jordan Canonical Form of a Product of Elementary S-unitary Matrices
Project code : MAT-18-1-05 Funding source : NSRI Amount of funding : 2018 – P 53,000.00 Duration of project : January 1, 2018 – December 31, 2018 Project leader : Dr. Agnes T. Paras
Objectives of the project:
The project aims to determine the Jordan canonical form (JCF) of a product A of two elementary S unitary matrices, when S is nonsingular and Hermitian. We expect that the JCF of A is one of only the following matrices
But we also wish to give necessary and sufficient conditions for when these occur.
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LIST OF COMMITMENT PROJECTS IN 2018
NSRI
1) Project title : Validation of DNA Typing Procedures and Assessment of Genetic Relationships among Filipino Ethnic/Language Groups
Project code : NSR-97-2-04 Funding source : NSRI/Office of the Chancellor, UPD Amount of funding : personnel salary Duration of project : continuing project since 2008 Project leader : Dr. Maria Corazon A. De Ungria (2008 – present) Personnel : 4 University Research Associate I
Status of the project:
Project NSR-97-2-04 has helped sustain and continues to sustain the research and extension operations of the DNA Analysis Laboratory, NSRI-UPD to date.
The four University Research Associates (URAs) under NSR-97-2-04 have been instrumental in validating DNA analysis procedures from pre-laboratory details to post- laboratory details. Notably, these URAs have been vital to DAL extension operations as they are part of the DAL’s DNA casework team which applies the validated DNA analysis procedures in actual DNA casework (paternity, kinship and human identification). With the assistance and support of NSRI Director, the laboratory was granted permanent items (loaned from other units). From four contractual positions, this was reduced to one University Research Associate (URA) under NSR-97-2-04. The other three University Research Associates (URAs) were given permanent items.
To further our research the laboratory has proposed a Filipino Genomes Research Program (FGRP). The FGRP proposal was submitted to the Philippine Council for Health Research and Development (PCHRD) of the Department of Science and Technology (DOST). The FGRP will include more Filipino RC and EL groups for greater representation of the Filipino population. The FGRP will leverage next generation sequencing (NGS) or massively parallel sequencing (MPS) technologies to generate DNA data that spans larger regions of and/or the entire human-Filipino genome. The project will address Filipino group representation and the largest Filipino genome coverage to date for various applications ranging from forensics, anthropology, Filipino history and evolution to health and medicine. The FGRP will also aid in the increase of the local scientific workforce as well as help in the expansion of research capabilities across agencies/institutions across the country. We have on-going collaborations with the National Institute of Health of the University of the Philippines Manila and Partner Institute for Computational Biology, Shanghai Institute of Biological Sciences, Shanghai on the data analysis of the 34 Negrito groups to better understand their culture.
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The support that NSR-97-2-04 provides is vital and will be vital to the research and extension operations of the DNA Analysis Laboratory, NSRI-UPD.
2) Project title : Chemical Waste Management Program of NSRI
Project code : NSRI-00-1-05 Funding source : NSRI Amount of funding : 2018 - P 260,000 (treatment and transportation of chemical waste and laboratory supplies and additional 1 URA I) 2017 - P 260,000 (treatment and transportation of chemical waste and laboratory supplies and additional 1 URA I) 2016 - P 260,000 (treatment and transportation of chemical waste and laboratory supplies and additional 1 URA I) 2015 - P 260,000 (treatment and transportation of chemical waste and laboratory supplies and additional 1 URA I) 2014 – P 260,000 (personnel salary, treatment of chemical waste and lab supplies) 2013 – P 260,000 (personnel salary, treatment of chemical waste and lab supplies)
Duration of project : continuing program since 2000 Project leaders : Dr. Windell L. Rivera Pollution Control Officer : Ms. Kristine O. Manalang, R.Ch. (2018-NCR-0330) Mr. Vergel G. Valenzuela, R.Ch. (2018-NCR-0327- QC1); DENR Hazardous Waste Generator ID 13-74- 0348 Chemical Safety and Waste Mgt. Associate : Mr. Adriel John R. Ilagan (contractual July 2018 - present)
Status of the project:
The NSRI is continuously generating chemical wastes from the operations of its research and technical laboratories. Thus, the NSRI is expected to sustain its chemical waste management program. In compliance with the requirements of RA 6969 (Toxic Substances and Hazardous and Nuclear Waste Control Act of 1990), the NSRI has been a DENR-registered hazardous waste generator since year 2000.
The main focus of the project is to monitor the waste generated at the Institute and to ensure safe storage until it is due for treatment by accredited waste treaters. For Y2018, it has continually implemented its safety guidelines regarding waste collection and storage such as proper identification of chemical wastes and proper documentation
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of chemicals during turn-over of laboratory rooms. The program has also conducted an inventory of its chemicals under the chemical exchange program. For Y2018, the program has also actively participated in the two safety seminars conducted by the Institute annually.
The program also handles and complies with the regulations and laws as enforced by the PNP, PDEA and EMB such as acquiring and renewing licenses and permits for the use of regulated and controlled chemicals, submission of consumption reports of those regulated chemicals and the self-monitoring reports to the aforementioned government agencies.
Table 1. Breakdown of Chemical Waste Management Program Y2018 activities & by the CWM Associate
ACTIVITIES DATE
Updated and submitted Self-monitoring reports (SMRs) to January, April, July and October DENR-EMB 2018
Updated the inventory and list of chemicals available for December 2018 the Chemical Exchange Program (CEP)
Disposal of chemical wastes in accordance to RA6969 December 2018
Obtained PDEA Purchaser License for Controlled Precursors December 7, 2018 and Essential Chemicals
Speaker, NSRI Safety Seminar entitled “Safety and Security February 26, 2018 and September in the Workplace: A Primer on NSRI’s Safety and Security 17, 2018 Rules and Guidelines”
Obtained PNP Permit to Purchase and Move Controlled September 6, 2018 Chemicals
Basic Pollution Control Officer Training July 2018
Obtained PNP Purchaser's License for Controlled Chemicals June 10, 2018
Table 2. Number of Requests for Chemical Exchange Program for 2018
CLIENT NUMBER OF REQUESTS
College of Science (CS) students/researchers 4
Non-CS students 9
Non-UP students 5
NSRI In-house Labs 1
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LIST OF EXTERNALLY – FUNDED PROJECTS IN 2018 (NSRI regular research staff received grants for the following externally funded projects)
1) Project title : Nutrient Depletion and Microbial Co-Culture of Chlorella vulgaris for Bioethanol Production
Funding Agency : NSRI – PCIEERD Amount of Funding : P 895,000.00 Duration of project : August 2017 – December 2018 Project Leader : Dr. Pierangeli G. Vital Graduate Assistant : Ms. Katherine L. Pintor
Description of the project:
The main objective of the study was to determine the effects of nitrogen and phosphorus deplete conditions on both growth and bioethanol production of an indigenous freshwater Chlorella vulgaris isolated from Pampanga, Philippines.
2) Project title : Philippine Environmental (Airborne and Surface) Germ Study and in Primary Schools and Homes
Funding Agency : P&G Singapore Amount of Funding : SGD 47,250.00 Duration of project : September 1, 2017 – December 31, 2018 Project Leaders : Dr. Pierangeli G. Vital Dr. Windell L. Rivera Research Associates : Ms. Aurora Ines Aguilar Ms. Samantha Valledor
Description of the project:
This study aimed to quantitatively assess bioaerosol levels in Philippine urban and rural primary schools and homes, to compare indoor and outdoor levels, to determine the diversity of airborne and surface bacteria and fungi, and to determine the seasonal impact (wet season, dry season, and Christmas/New Year season) to abundance and diversity.
3) Project title : Assessment of Microorganisms from Commercially Available Probiotic Products Suitable for Children being Marketed in the Philippines
Funding Agency : OVCRD Amount of Funding : P 300,000.00 Duration of project : April 1, 2018 – April 1, 2019 Project Leaders : Dr. Pierangeli G. Vital Graduate students : Ms. Clarizza May Dioso
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Description of the project:
The study aimed to evaluate the microorganisms from commercially available probiotic products for children sold in the Philippines. In the Philippines, there is a high mortality rate for infants and children due to persistent and acute watery diarrhea. Therefore, doctors are now looking at probiotics as adjunct treatment to fight off this deadly diarrhea in children. A wide range of probiotic products are available in the market and can easily be bought without a medical prescription.
4) Project title : Field integrated novel diagnostics for flaviviruses (FIND FLAVIVIRUSES)
Funding Agency : PCHRD-DOST Amount of Funding : P 14,805,956.00 Duration of project : January 15, 2018 – January 14, 2021 Project Leader : Dr. Raul V. Destura Co-investigators : Ms. Joy Ann P. Santos, MSc Graduate student : Mr. Adrian Abarientos
Description of the project:
To develop and optimize laboratory performances of a 2-step pan-Flaviviruses RT-LAMP assay followed by type-specific flavivirus identification panel testing for the field application.
Target organisms: Dengue (DENV), Zika (ZIKV), Japanese Encephalitis (JEV) and Chikungunya(CHIKV)
5) Project title : Development of Low Cost Point of Care Diagnostics for Simultaneous Detection of Paragonisimus westermani and Mycobacterium tuberculosis Using RPA Technology
Funding Agency : PCHRD-DOST Amount of Funding : P 4,994,050.60 Duration of project : July 1, 2018 – June 30, 2019 Project Leader : Mr. Angelo de la Tonga, MSc Co-investigators : Ms. Joy Ann P. Santos, MSc Research associates : Ms. Stessi Marie Geganzo Ms. Melanie Salinas
Description of the project:
The main objective of the study is to develop a low-cost point of care diagnostic kit for the detection of Paragonimus westermanii and Mycobacterium tuberculosis infections using nanogold technology
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6) Project title : Basic Molecular Biology Techniques for Teachers and Researchers
Funding Agency : OVCRD - OEC Amount of Funding : P 100,000.00 Duration of project : December 2018 – December 2019 Project Leaders : Dr. Pierangeli G. Vital and Ms. Joy Ann P. Santos, MSc
Description of the project:
The project revived and revised the molecular biology workshop being offered by the Biological Research and Services Laboratory. It is now offered again after five years.
7) Project title : Assessment of a Locally Developed Loop-mediated Isothermal Amplification Assay for the Diagnosis of Schistosoma japonicum infections in Selected Areas in the Philippines
Amount of Funding : P 2,999,999.00 Duration of project : January 1, 2018 – May 31, 2019 Project Leader : Dr. Vicente Y. Belizario Jr. Consultant : Ms. Joy Ann P. Santos, MSc Research Associates : Ms. Olivia T. Sison, MSPH Mr. John Paul Caesar R. delos Trinos, MPH, MHM Ms. Jana Denise M. Lacuna, RMT Ms. April Joy E. Gigataras, RMT Mr. Adrian B. Abarientos
Description of the project:
The study aimed to assess the utility of a locally developed LAMP assay for the diagnosis of schistosomiasis in selected areas in the Philippines.
8) Project title : Genotoxicity and Antigenotoxicity Evaluation of Bark Extracts of Muntingia calabura L.
Funding source : OVCRD Amount of funding : P 300,000.00 Duration of project : September 1, 2017 – extension Project leader : Ms. Raquel O. Rubio
Description of the project:
Traditional medicine uses different parts of plants to search for substances that could be developed as cytotoxic, antioxidant, antimicrobial and anticancer drugs. Antioxidants are compounds which have the ability to scavenge or trap free radicals.
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Flavonoids and phenolics derived from plants that have antioxidant activities can inhibit the propagation of free radical reactions and protect the human body from metabolic diseases due to oxidative stress. Free radicals include the reactive oxygen species which, at high concentrations, could become harmful and damage DNA, lipids and proteins. In the Philippines, the antioxidant potentials of many of the reported medicinal plants remain largely unexplored. Muntingia calabura L., known in English as Jamaica cherry or ‘aratiles’ in the Philippines, is commonly used in traditional folkloric medicine against various afflictions. To assess its genotoxic and antigenotoxic potential, the comet assay and Allium cepa test will be employed.
9) Project title : Developing a Comprehensive Laboratory Animal Workshop for Teachers, School Administrators and Researchers
Funding source : OVCRD – OEC Amount of funding : P 100,000.00 Duration of project : December 1, 2016 – May 31, 2018 Project leader : Raquel O. Rubio
Description of the project:
Pursuant to the Animal Welfare Act of 1998 and Administrative Order 40 of the Department of Agriculture on the “Rules and Regulations on the Conduct of Scientific Procedures Using Animals,” a basic one-day laboratory training course was formulated by the Biological Research and Services Laboratory (BRSL), serving researchers and high school teachers from the National Capital Region and surrounding provinces who will be administering experiments on laboratory animal models. Presently, there is a need to reach more end-users from a greater breadth of regions. Most importantly, in response to the feedback from previous workshop attendees, the scope of the training needs expansion on the breadth of topics covered, which include more animal models, more recent laboratory techniques, and greater depth in practical training with regards to the creation of Institutional Animal Care and Use Committee (IACUC) in different institutions. At present, almost all animal model researchers do not have IACUC in their own schools or institutions, a requirement under AO 40 to prevent inhumane treatment of experimental animals. Hence, it is proposed that the current Laboratory Animal Workshop be expanded to more comprehensively address these concerns.
10) Project title : Sama Origins and Transformations: Evidence from Genes, Languages and Cultures
Funding source : Commission on Higher Education Amount of funding : P 10,000,000.00 Duration of project : July 2018 – July 2020 Project leader : Dr. Maria Corazon A. De Ungria
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Description of the project:
The overall objective of the study is reconstructing the origins and transformations of Sama peoples through time, the project draws strength from multiple disciplines namely, Human Population Genetics, Native Animal Population Genetics, Historical Linguistics, and Cultural Anthropology.
11) Project title : The Reconstruction of Jose Rizal's DNA to Study His Phenotype, Genealogy and Genetic Ancestry
Funding source : UP - OVPAA Amount of funding : P 2,300,000.00 Duration of project : April 2013 – June 2017 (analysis on-going) Project leader : Dr. Maria Corazon A. De Ungria
Description of the project:
The overall objective of the study is to use bioinformatics to reconstruct the genealogy, genetic ancestry and selected phenotypic characteristics of our national hero, Jose Rizal, using the DNA of his living relatives. The project is exploratory and aims to test the feasibility of this approach in studying the genetic ancestry, genealogy and other traits of other Filipino decedents. New bioinformatics tools that will be developed and used for the project will also be made available.
12) Project title : Palaeoenviromental and Biodiversity Study of Mindoro Island: An Archaeological Science Initiative Project
Funding source : UP – OVPAA - EIDR Amount of funding : P 800,000.00/year (for the genetics component of the study) Duration of project : 2012 – 2017 (analysis on-going) Project leader : Dr. Armand Mijares Co-Project leader : Dr. Maria Corazon A. De Ungria
Description of the project:
This palaeoenvironment and biodiversity study is an interdisciplinary project that addresses a standing need for palaeoecological basic research to be directly linked to ancient and current biodiversity questions and early human movement in island SE- Asia. There are very few research projects in the entire Southeast Asian region that attempt to cover these topics together. By initiating this research we are confident that there will be patterns coming out of the palaeoecological data that may help us understand past and current environmental and biodiversity issues and human adaptations.
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The human genetics component of the project will look into ancient human history of Mindoro island by examining the genome-wide Single Nucleotide Polymorphisms, autosomal and Y-chromosomal Short Tandem Repeats of the participating Mangyan communities. The genetic information obtained will supplement linguistic and archaeological data to address questions on human migration and distribution as well as the behavioural adaptations to the palaeoenvironmental conditions faced by the early inhabitants of Mindoro. The findings of this research will contribute not only to the ancient history of Mindoro but also to the early human history of the Philippine archipelago and by extension, island South East Asia.
13) Program title : Discovery and Development of Health Products: Marine Component (DDHP: Marine)
Project title : Project 1: High-throughput Multi-omics Discovery of Bioactive Marine Compounds and Establishment of a Marine Chemical Library and Informatics System
Study title : Characterization and Culture of Microbial Isolates
Funding source : Department of Science and Technology-Philippine Council for Health Research and Development (DOST-PCHRD) Amount of funding : P 16,350,947.00 (for the entire project) Duration of the project : July 1, 2017 – March 31, 2018 Program leader ; Dr. Arturo O. Lluisma Project leader : Dr. Arturo O. Lluisma Project staff, level 3 : Dr. Maria Auxilia T. Siringan
Abstract:
The Discovery and Development of Health Products: Marine Component (DDHP:MC) has established culture collection of sponge-associated marine microorganisms (MMOs). The collection consists of 1,446 microbial isolates derived from marine sponges collected in Puerto Galera, Oriental Mindoro, Zamboanga del Sur and Bolinao, Pangasinan and Cebu from 2014 to 2016. These microbial resources are vital infrastructure for the establishment of the DDHP chemical library.
MMOs with antimicrobial activity against one of the six test “ESKAPE” pathogens, specifically, Enterobacter aerogenes ATCC 35029, Staphylococcus aureus ATCC 6538, Klebsiella pneumonia ATCC 13883, Acinetobacter baumannii NCIMB 12457, Pseudomonas aeruginosa ATCC 27853, and Enterococcus faecium ATCC 6569 and/or anti-proliferative activity against human cancer cell lines, HCT116 and MCF7 were designated as priority MMOs. Priority MMOs Microbial cultures were subjected to long- term preservation methods (L-drying, lyophilization and storage as glycerol stocks at - 80oc), phenotypic characterization and 16s rDNA sequencing-based identification.
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For the bioactivity testing, methanolic fractions from small scale cultures of the priority MMOs were screened for antimicrobial activity using the above-mentioned six test bacteria and the optimized Microplate Alamar Blue Assay (MABA). Fractions were subjected to anti-proliferative assay and chemical profiling conducted by the Chemistry group of Project 1.
16S rDNA sequencing-based identification of 351 MMOs revealed the following: a) forty-one (41) genera were represented, b) 22.22% (78/351) of the MMOs belong to the genus Bacillus; c) about 22% (77/351 or 21.94%) were named as Staphylococcus, d) more than 10% (37/351 or 10.54%) were identified as species of Vibrio, e) 8.83% (31/351) were classified under the genus Nesiotobacter, f) 4.84% (17/351) were named as species of Pantoea, and g) 2.85% (10/351) were placed under the genus Paenibacillus. Other genera represented among the priority MMOs include, Brevibacterium, Brachybacterium, Catenococcus, Corynebacterium, Ferrimonas, Flavobacterium, Haloarcula, Halobacillus, Kangiella, Loktanella, Lysinibacillus, Marinobacter, Marinobacterium, Mesoflavibacter, Microbulbifer, Micrococcus, Natronococcus, Nocardia, Parvularcula, Phaeobacter, Photobacterium, Pseudomonas, Pseudovibrio, Psychrobacter, Ruegeria Salegentibacter, Salinicola, Salinivibrio, Streptomyces, Terrabacter, Tersicoccus Verrucosispora, and Virgibacillus.
Prevalence of Gram-positive bacteria was noted. 16S rRNA sequencing for taxonomic assignments of 251 Y1 andY3 DDHP MMOs is in progress. To our knowledge, except for Bacillus and Vibrio, this is the first report on the isolation of these bacterial genera, from Philippine marine sponges.
Of the 351 MMOs identified using the 16s rDNA sequencing, 274 MMOs (78.06%) have bioactivity against the ESKAPE pathogens. About 37% (36.9% or 101/274) exhibited anti E. faecium activity while 33.6% (92/274) showed activity against S. aureus. Based on the consolidated results of the antimicrobial assay, anti- proliferative assay and chemical profiling, MMOs with promising chemistry were prioritized for bioactive compound production by Project 1 Chemistry Group and culture optimization studies by Project 3.
14) Program title : Discovery and Development of Health Products: Marine Component (DDHP: Marine)
Project title : Project 3: Discovery and Development: Anti- Infectives and Anti-Cancer
Study title : Microbial Culture Optimization
Funding source : Department of Science and Technology-Philippine Council for Health Research and Development (DOST-PCHRD) Amount of funding : P 8,401,009.00 (for the entire project) Duration of the project : July 1, 2017 – March 31, 2018
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Program leader ; Dr. Arturo O. Lluisma Project leader : Dr. Lilibeth S. Reyes Project staff, level 3 : Dr. Maria Auxilia T. Siringan
Abstract:
Under the DDHP: MC Program, Project 3 aims to discover, characterize and develop anti-infective and/or anticancer compounds from sponge-associated marine microorganisms (MMOs). To harness the bioactive potentials of the MMOs, the physico- chemical and nutritional requirements for their growth and production of the target bioactive metabolites were determined and established. Optimization of these conditions was imperative to increase the yield of the desired compound. This was undertaken by cultivating the microorganisms under varying physico-chemical parameters and media composition.
Ten (10) sponge-associated marine microorganisms were prioritized by Project 3 Chemistry group for culture optimization studies, bioactivity testing and chemical profiling which include the following: a) eight (8) DDHP MMOs, namely, PGI055, PGR010, PGR032, PGR053, PGR080, PGR096, ZMR011 and ZMR029 and b) two (2) PHARMASEAS filamentous bacteria, R2A-788-1-A1 (788) and R2A-818-A (818).
Six MMOs were subjected to growth studies to determine the set of culture conditions which can enhance growth and production of target bioactive compounds. The following are the candidate empirically determined optimum growth conditions for each MMO: 1) for R2A-818A Amycolatopsis sp.: R2A broth with 1% NaCl, 30°C,initial pH: 7.0; 0.5 g/L L-pyroglutamic acid as carbon (C) source and 0.5 g/L peptone as nitrogen (N) source; 2) for R2A-788 Streptomyces carpaticus: R2A broth with 0% NaCl, 25°C; initial pH: 7.0, 0.5 g/L glucose or 0.5 g/L soluble starch as C source and 1.0 g/L L-glutamic acid as N source; 3) for R2A-613B Pseudomonas aeruginosa: R2A with 3.5% NaCl grown at 25oC and R2A without NaCl grown at 30oC; 4) for PGR010 Pantoea dispersa: R2A broth with 0% NaCl, 25°C, initial pH: 7.451, 1.5 g/l L-glutamic acid as C-source, and 0.5 g/l peptone as N-source; 5) for PGR080 S. saprophyticus: R2A broth with 1% NaCl, 30°C, initial pH: 7.5, 1.5 g/l L-glutamic acid as C source and 0.5 g/l peptone as N-source; and 6) for ZMR011 Bacillus aryabhattai: R2A broth with 0% NaCl, 30°C, pectin as candidate C source and xanthine as candidate N source.
Isolate 818 was prioritized for extensive culture optimization using the Response Surface Methodology (RSM) approach. Isolate 818 was shown to produce harmane, a known compound but could possibly exhibit a new bioactivity against the colorectal cancer cell line HCT116. Culture conditions that were selected for the optimum growth of 818 include 30oC as incubation temperature, 1% NaCl, L-pyroglutamic acid as carbon source and peptone as nitrogen source. These growth parameters were employed in the RSM-guided culture optimization studies, where L-pyroglutamic acid replaced dextrose and starch as carbon source and peptone is the sole organic nitrogen source. Based on RSM-guided studies, using the empirically determined combination of 30oC as incubation temperature and 1% NaCl, the following set of growth parameters were
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predicted to increase either the yield of extract or bioactivity: a) pH 7.998. 1.5 g/L L- pyroglutamic acid, and 1.5 g/L peptone to generate 4.813 µg/100 mL medium or a 0.19 fold increase in the yield; b) pH 8, 1.267 g/L L-pyroglutamic acid and 0.5 g/L peptone to generate 17.885% inhibition of HCT116 or a 0.805 fold increase in anti-HCT116 activity. The latter predicted culture conditions were selected in order to reduce the predicted activity against the normal cell line, AA8. Validation of the selected RSM treatments which initially generated fractions that inhibited HCT116 cells was further conducted. Results of the validation studies showed that all 6 treatments showed promising anti- HCT116 activity, ranging from 36.05 to 51.26%. In large scale production of target metabolite, it is recommended to reduce the cost of operations and thus, treatment 9 conditions, which include an initial pH of 7, 0.5 g/L L-pyroglutamic acid 0.5 g/L peptone could be employed. With lower levels of carbon and nitrogen sources, production of the target molecule produced by 818 will be less expensive.
When optimum NaCl concentration, incubation temperature and nutritional requirements are identified for each priority MMO, application of appropriate software such as Plackett Burman Design (PBD) and Response Surface Methodology (RSM) and Central Composite Design (CCD) was useful in monitoring the effects of the parameters on the growth and production of the target metabolite.
15) Project title : Microbial Diversity in the Long-term Fertility Experiment in Irrigated Lowland Rice Ecosystem
Study title : Isolation of Plant-growth Promoting Bacteria Associated with the Soil-rice Rhizosphere Ecosystem
Funding source : Department of Agriculture- Regional Field Office 3 (DA-RFO3) Amount of funding : P 220,000.00 (for the Microbiology Component) Duration of project : September 1, 2017 – June 30, 2019 Project Leader : Ms. Jayvee A. Cruz Researcher : Dr. Maria Auxilia T. Siringan
Abstract:
Microbial communities in long-term fertility trials in irrigated lowland rice ecosystem and in unfertilized rice fields were evaluated to determine their contributions to long-term trends in the rice yield gap between the potential yield and the actual yield, at the different stages of rice production. The project aims to assess the functional diversity of the microbial communities in long-term soil fertility experimental trials (LTFE) in irrigated lowland rice ecosystem and in less intensive or unfertilized rice cropping systems. Functional microbial diversity of the rice-ecosystem was determined during the vegetative, reproductive, and ripening stages of rice production, using BiOLOG EcoPlates, GenIII BiOLOG identification system and Microlog 4.01 software (Biolog Inc., Hayward, CA, USA). Data generated from the functional diversity profiling are currently being analyzed using appropriate statistical analyses. Spatial and temporal variabilities in microbial communities will be analyzed using
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modern experimental designs such as Geostatistics. The relationship between changes in the metabolic diversity and soil fertility, including soil nutrient supply, fertilizer management and rice crop productivity will be investigated.
In this study, bacteria associated with soil-rice rhizosphere ecosystem were isolated and screened for plant growth-promoting activities such as the ability to produce indole-3-acetic acid (IAA), phosphate solubilization activity and nitrogen-fixing potential determined through a nifH gene-targeting PCR assay. Phenotypic and genotypic characterization studies of the promising isolates are in progress. Promising isolates will be preserved using long-term preservation method (e.g., L-drying, freeze- drying, storage under ultralow freezing temperature) and will be maintained at the University of the Philippines Culture Collection (UPCC), MRSL, NSRI in UP-Diliman.
16) Project title : Perfluorinated Compounds in Water, Sediments, Biota and Drinking Water in the Philippine Environment
Funding source : United Nations University Institute for the Advanced Study of Sustainability (UNU-IAS) in cooperation with Shimadzu Corporation, Japan. Amount of funding : USD 4,000 Duration of the project : January 2 – May 31, 2018 (UNU-IAS initiated extension for Phase VII - Year 2017) August 1 - December 31, 2018 (Phase VII - Year 2018) Project Leader : Dr. Charita S. Kwan (National Project Coordinator) Project Staff : Ms. Kristine O. Manalang (additional assignment) Ms .Jannelle O. Cristobal (additional assignment Mr. Vergel G. Valenzuela (additional assignment)
Description of the Project:
The project on perfluorinated compounds (PFCs) in the Philippine environment is part of a larger project on “Monitoring and Management of Persistent Organic Pollutants (POPs) in Asia” initiated by the United Nations University since 2005. The project has established a POPs monitoring network among ten Asian countries and has assisted in the capacity building of these countries to measure POPs in their respective environments, wherein the data can be utilized in the assessment of the effectiveness of the Stockholm Convention (SC) on POPs, a global regulation that entered into force in May 2004.
POPs constitute several classes of chemicals that are toxic, can bioaccumulate, can persist in various compartments of the environment and are capable of long-range environmental transport, i.e., the ability to cross national boundaries. Perfluorinated compounds have been chosen as the classes of chemicals to be monitored in the Phase VII of the project, i.e., since 2016. Among the PFCs, perfluorooctane sulfonic acid (PFOS), its salts, and perfluorooctane sulfonyl fluoride (PFOS-F) are listed as among the 16 new POPs in the Stockholm Convention in 2017. In 2018, perfluorooctanoic acid
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(PFOA) and perfluorohexane sulfonic acid (PFHxS) and their corresponding salts and related compounds have also been proposed for listing under the Stockholm Convention. Thus, the participation of the Philippines through the UP NSRI RASL in the UNU-IAS regional project on the monitoring of PFCs would provide important information on the occurrence of these chemicals in the Philippine environment.
The environment of Manila Bay and Laguna Lake have been chosen as the sampling areas for the monitoring of PFCs since 2016. Both are important bodies of water in the Philippines because of their economic contributions including fisheries or food production for the population of the Greater Metropolitan Manila Area and the nearby provinces. However, due to their proximity to the Greater Metropolitan Manila Area, these bodies of water are continuously impacted by industrialization, urbanization and rapid population growth, which are known precursors of pollution. Moreover, monitoring of PFCs in Laguna Lake is important as it is one of the sources of drinking water for some areas of the Greater Metropolitan Manila Area.
Due to budgetary constraint, only five tributary rivers of Manila Bay (Meycauayan, Pasig, Marikina, Malimango and Ylang-ylang Rivers) and three tributary rivers of Laguna Lake (San Juan, San Cristobal and Pangil Rivers) have been chosen as the sampling sites. Most of these rivers are suspected recipients of PFC pollution both from domestic and industrial sources, except for the Pangil River which has been identified as the background site. The head waters of Pangil River is located in a forested area in Laguna; thus, it may provide the baseline level of PFCs for an unpolluted site in the Philippines.
The concentrations of nine PFCs: PFOA, PFOS, PFBA, PFHxA PFNA, PFDA PFUnDA, PFDoDA, PFHxS were determined in the water and sediment samples collected from the selected tributary rivers. To examine potential routes of human exposure to PFCs, these were also measured in fish species that are known to be abundant or commonly caught in Manila Bay and in Laguna Lake for human consumption, i.e., the mackerel (Rastrelliger kanagurta and Rastrelliger brachysoma) from Manila Bay and the milkfish (Chanos chanos) and tilapia (Oreochromis niloticus) from Laguna Lake, and in drinking water samples from selected locations in Metro Manila that are known to be supplied by the two water concessionaires including one location where the drinking water is treated water from Laguna Lake.
Country reports have been submitted to UNU-IAS for the year 2017-2018 and for year 2018. An oral presentation and a poster paper on the results of the study in 2017-2018 was presented at the 27th Symposium on Environmental Chemistry organized by the Japan Society for Environmental Chemistry in Okinawa, Japan on May 24, 2018.
17) Project title : Global Atmospheric Sampling (PUF-disk) – Activities in the Philippines
Funding source : Environment and Climate Change Canada, Government of Canada – Air Quality Research Division, Science and Technology Branch
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Amount of funding : None (Provision of sampling materials and arrangements with courier for dispatching of samples to Canada) Duration of project : January 1, 2018 – December 31, 2018 Project Leader : Dr. Charita S. Kwan (Implementor – Philippines) Project Staff : Ms. Kristine O. Manalang (additional assignment) Mr. Vergel G. Valenzuela (additional assignment)
Description of the project:
The Global Atmospheric Passive Sampling (GAPS) network consisting of more than 50 sites across seven continents has been initiated by the Government of Canada through the Environment and Climate Change Canada, Air Quality Research Division (ECCC-AQRD)) since 2005. The main objective of the program is to produce comparable data for persistent organic pollutants (POPs) on a global scale. Passive air samplers (PAS) utilizing polyurethane foam (PUF) disks as sampling media are deployed by participants of the GAPS network on a quarterly basis, wherein the concentrations of both legacy and emerging POPS and other priority pollutants are measured in the PUF disks by ECCC-AQRD. The deployment of PAS worldwide for many years allows the collection of data for the assessment of regional and global long-range atmospheric transport of POPs, and as well as the effectiveness of the regulations and agreements under the Stockholm Convention.
The data on POPs for the Philippines under the GAPS network is taken from the PAS installed at the NSRI rooftop by the Research and Analytical Services Laboratory of NSRI. Our inclusion in the GAPS network provides the opportunity for the Philippines to obtain baseline information on the levels of legacy and emerging pollutants in the Philippine atmosphere, wherein measurement of most of these chemicals cannot be done readily due to logistical support especially on the availability of chemical standards. Among the important information gathered by this study is the first global- scale distributions of 16 non-polybrominated diphenyl ether flame retardants which included data for the Philippines (Lee et al. (2017). Environmental Pollution 217, 62-69).
18) Project title : Developing Information Infrastructure for Managing Antibiotics and Endocrine Disrupting Substances in Pampanga River Basin and its Coastal Environs: Maps, Transport Models and Bioindicators of Ecological and Public Health Risks
Funding Source : Commission on Higher Education – Philippine California Advance Research Institute (CHED-PCARI) Amount of Funding : P 50,250,199.67 (for the entire project) Duration of the Project: June 16, 2019 (start of a two-year project) Project Leader : Dr. Arturo O. Lluisma (UP Marine Science Institute) Co-Project Leader : Dr. Analiza P. Rollon (UP Department of Chemical Engineering) Environmental Chemistry Component Project Staff, Level 3 : Dr. Charita S. Kwan Research Fellow : Ms. Aira Sacha Nadine S. Ferrer
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Description of the project:
This research project aims to address the growing concerns on the negative effects of micropollutants, such as endocrine disrupting compounds (EDCs) and antibiotics, on the organisms that inhabit lotic ecosystems as well as ecosystems surrounding them, including human communities. Because of the serious threat posed by these micropollutants on human and ecosystem health, it is imperative that this project accomplishes its goals to provide the public of more information about the sources, levels and distribution of micropollutants in one of the main lotic ecosystems in the Philippines, the Pampanga River Basin.
To achieve the project’s goal of understanding and providing information on the sources, levels and distribution of micropollutants as well as their immediate effects on the biotic components of the lotic ecosystems, the project is divided into different components: (1) chemistry, (2) ecology, (3) metagenomics, and (4) modelling. The chemistry component of the project aims to characterize and identify the micropollutants present along the Pampanga River Basin. The ecology component of the project aims to collect and identify plankton, benthic macroinvertebrates and fish that inhabit the Pampanga River Basin. Additionally, the component also aims to survey mangrove areas present along the Pampanga River delta opening to Manila Bay. The metagenomics component of the project aims to identify the diversity of bacteria present in sediment and water samples collected from the lotic systems. In addition to this, this project component is also responsible for determining whether there are any drug-resistant strains of bacteria present in the samples. Lastly, the modelling component of the project aims to collect all physical and chemical data on the Pampanga River Basin and project these data on a modelling software to show the levels, sources and fates of the micropollutants and their effects on surrounding ecosystems.
The project wants to address the urgent need to determine the sources, levels, and distribution of micropollutants in the environment, as well as their impacts and risks on environmental and public health. It will collectively look at mapping sources, modelling transport, determining bioindicators of micropollutant impacts, and developing an information platform for micropollutant levels and associated ecological and public health risks. All these information should be made available for public use to benefit policy makers and government agencies, the public health research sector, the scientific community and the general public.
19) Project title : Strengthening Knowledge of Chemical Safety and Waste Management in Academic Laboratories: A Service-Learning Project of the UP NSRI Research and Analytical Services Laboratory for Public Secondary Schools
Funding source : UP OVCRD Amount of funding : P 100,000.00 Duration of project : December 1, 2018 (start of a one-year project)
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Project Leader : Ms. Jonah L. Bondoc Supporting Staff : Mr. Charita S. Kwan (additional assignment) Mr. Vergel G. Valenzuela (additional assignment) Ms. Kristine O. Manalang (additional assignment)
Description of the project:
The concept of the project was founded on the onset of the K-12 program, particularly the STEM program, in the Philippines. The project aimed to re-educate the high school science teachers on the culture of safety in the laboratory. In return, it is envisioned that these science educators will pass on the knowledge of safety in the science laboratories to their junior and senior high school students. Initially, two national high schools were chosen as pilot schools for the project, the Marcelo H. Del Pilar National High School in Bulacan and the Capiz National High School in Roxas City.
20) List of PCIEERD-NSRI Research Projects for 2017-2018 (extended up to December 2018)
a) Project Title : Establishment of Nationwide Scheme for Sampling Marine Mammal Tissues and Pilot deployment of Hydrophones for Surveillance of Dynamite Blasts
Project code : PCIEERD-NSRI-ESM-17-01 Funding source : PCIEERD Amount of funding : P 895,000.00 Duration of project : July 1, 2017 – December 31, 2018 Project leader : Dr. Lemnuel V. Aragones Co-Project Leader : Dr. Marie Christine M. Obusan Collaborator : Dr. Giovanni Tapang Personnel : 1 Research University Associate I
Objectives of the project:
This project aims to employ relevant technological applications for optimizing opportunities provided by stranded marine mammals throughout the Philippines.
The specific objectives are:
1) Establish a nationwide scheme for sampling marine mammal tissues for collection and storage; and 2) Pilot subsurface acoustics surveillance for monitoring underwater noise, including dynamite blasts.
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b) Project Title : Development of Climate Envelope Models to Assess Philippine Food Security Under IPCC Climate Change Scenario
Project code : PCIEERD-NSRI-ESM-17-02 Funding source : PCIEERD Amount of funding : P 895,000.00 Duration of project : July 1, 2017 – December 31, 2018 Project leader : Dr. Benjamin M. Vallejo, Jr. Personnel : 1 Research University Associate I
Objectives of the project:
The project aims to develop a climate envelope models (CEM) using ecological forecasting approaches that can be used for assessing climate change risks to food security under the IPCC climate change scenarios.
We intend to use climate envelope models to come up with predictive distributional habitat suitability for 1) rice, 2) corn, 3) cruciferous vegetables, 4) mango and 5) Tilapia. This will be cartographically represented.
Under the IPCC scenarios, we hypothesize a distributional shift in habitat suitability, likely on a latitudinal scale for thermally sensitive food crop species and a longitudinal shift for species dependent more on rainfall patterns.
The project also plans to investigate the basic science biogeographic spatial statistical problem of CEM which is on scaling relations of presence only modeling. c) Project Title : Metagenomic Sequencing of Cyanobacteria from Some Mining Sites in Benguet Province for Genome Mining of Stress Tolerance Genes
Project code : PCIEERD-NSRI-BIO-17-01 Funding source : PCIEERD Amount of funding : P 895,000.00 Duration of project : July 1, 2017 – December 31, 2018 Project leader : Dr. Ernelea P. Cao
Objectives of the project:
The general objective of this project is to study the genomes of cyanobacteria from some mining sites in Benguet Province and identify putative genes that may be responsible for the ability of these microorganisms to survive in highly-stressed environments. Elucidating the genetic basis for stress tolerance can also be a spring board for using these organisms as models for studying adaptations to changing environments.
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d) Project Title : Optimization of Environmental DNA (eDNA) Detection of Schistosoma japonicum and Oncomelania hupensis quadrasi from Laboratory and Field Water Samples for Schistosomiasis japonica Biomonitoring and Surveillance
Project code : PCIEERD-NSRI-BIO-17-02 Funding source : PCIEERD Amount of funding : P 895,000.00 Duration of project : July 1, 2017 – December 31, 2018 Project leader : Dr. Ian Kendrich C. Fontanilla Collaborator : Dr. Lydia Reyes Leonardo Personnel : 1 Research University Associate I
Objectives of the project:
To be able to detect environmental DNA of the cercariae of the parasite Schistosoma japonicum and the snail intermediate host Oncomelania hupensis quadrasi. e) Project Title : Polyethylene-degrading and Laccase-producing Bacteria from the Highly Polluted MMORS: Future Application for Industrial and Environmental in situ Pollutant Degradation
Project code : PCIEERD-NSRI-BIO-17-03 Funding source : PCIEERD Amount of funding : P 895,000.00 Duration of project : July 1, 2017 – December 31, 2018 Project leader : Dr. Marie Christine M. Obusan Collaborator(s) : Dr. Jessica F. Simbahan Dr. Windell L. Rivera Personnel : 1 Research University Associate I
Objectives of the project:
The proposed project generally aims to screen bacteria found in the sediments of Marilao-Meycauayan-Obando River System (MMORS) for pollutant biodegration. Specifically, it aims to:
1) Isolate bacteria that can degrade low density polyethylene (LDPE); 2) Test the LDPE-degrading isolates for laccase activity; 3) Confirm identification of Iolates using phenotypic and molecular technique; and 4) Analyze LDPE-degradation and laccase activities of identified bacteria in association with in-vitro condition and physic-chemical parameters of the river.
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f) Project Title : Microbial Prospecting of Mount Banahaw for Actinomycetes with Pharmaceutical Industry Applications
Project code : PCIEERD-NSRI-BIO-17-04 Funding source : PCIEERD Amount of funding : P 895,000.00 Duration of project : July 1, 2017 – December 31, 2018 Project leader : Dr. Gil M. Penuliar
Objectives of the project:
The number of drug resistant microorganisms continues to increase. This threatens the viability of therapeutic agents currently used in clinical practice. lf not addressed, the situation will eventually lead to increased morbidity and mortality, particularly among the elderly and immuno-compromised.
Very few microbiological studies have been done in protected areas in the Philippines. It is hypothesized that novel species of actinomycetes can be isolated from these areas, and that these species can produce secondary metabolites that have strong antimicrobial activity against drug resistant microorganisms. These secondary metabolites could be developed into alternative therapeutic agents that might be of great importance in clinical practice in the future.
The general objectives of this proposal are (a) to isolate actinomycetes from Mount Banahaw, and (b) to test the utility of their secondary metabolites for possible therapeutic applications. g) Project Title : Bioactivity - Guided Chromatographic Characteri- zation of the Aerial Parts of Origanum vulgare L., a Promising Source of Bioactive Secondary Metabolites
Project code : PCIEERD-NSRI-BIO-17-05 Funding source : PCIEERD Amount of funding : P 895,000.00 Duration of project : July 1, 2017 – December 31, 2018 Project leader : Dr. Juliana Janet M. Puzon Collaborator : Dr. Demetrio L. Valle, Jr. Personnel : 1 Research University Associate I
Objectives of the project:
1) To determine the presence of bioactive secondary metabolite classes in extracts and fractions from the leaves and stems of O. vulgare L. 2) To evaluate the antimicrobial activity, antioxidant activity and toxicity of O. vulgare L. extracts and fractions derived from the leaves and stems.
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3) To characterize the bioactive extracts and fractions through various chromatographic techniques. h) Project Title : Development of Aptadetection and Aptaprecipitation assays for Trichomonas vaginalis
Project code : PCIEERD-NSRI-BIO-17-06 Funding source : PCIEERD Amount of funding : P 895,000.00 Duration of project : July 1, 2017 – December 31, 2018 Project leader : Dr. Windell L. Rivera Collaborator : Dr. Clara O' Sullivan
Objectives of the project:
To evaluate the use of an aptamer as antibody alternative in the detection and precipitation assays for Trichomonas vaginalis. Specifically, this study aims to:
1) Compare the performance of the aptadetection assay for T. vaginalis
a. in different matrices particularly synthetic vaginal secretion, semen and urine; and b. with variable cell concentration-primarily of T. vaginalis, related trichomonads and other agents of sexually-transmitted infections (STI).
2) Assess the utilization of aptaprecipitation assay through identification of the aptamer - captured molecules.
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1.2 PUBLICATION AND CREATIVE WORKS
The NSRI regularly publishes the following:
1) NSRI Annual Report. This is a compilation of abstracts of NSRI-supported researches and includes a summary of the Institute's extension services for the year covered.
2) NSRI Catalogue of Cultures. This describes the culture collection holding of the Microbiological Research and Services Laboratory and is updated every two years.
3) R & D Services. a) Brochure of NSRI Research and Technical Services b) Catalogue of Training Courses uploaded in the NSRI website.
4) Scientific Publications Based on NSRI-Funded and NSRI-Assisted Projects. The output of NSRI supported projects are published by researchers in scientific journals. The 2018 publications based on NSRI-funded projects and other projects housed at NSRI are shown below (Table 3).
Table 3. 2018 Publications based on NSRI researches and other projects housed at NSRI
AUTHOR TITLE PUBLICATION
BIOLOGY Vital, P.G., Rivera, W.L., Microbial Assessment of Fresh, Environ Dev Sustain Abello, J.J.M. and Francisco, Minimally Processed Vegetable from DOI 10.10007/S10668-017-0022-x J.C.E. Open Air Markets and Supermarkets in Luzon, Philippines
Justo, A.C., San Diego, Metronidazsole Susceptibility and ACTA Medica Philippina M.A.C.V.R and Rivera, W.L. TVV-Infection of Trichomonas 52(6): 553-556 vaginalis from Metro Manila and Angeles City, Philippines
Obusan, M.C.M., Aragones, Antibiotic Susceptibility Patterns of Aquatic Mammals L.V., Rivera, W.L. and Siringan, Bacteria Isolated from Cateceans 44(5): 568-579 M.A.T. Stranded in the Philippines
Justo, C.A.C., Mapile, M.R.F., Determination of the Optimal Pre- Philippine Science Letter Santos, P.D.M. and Rivera, W.L. enrichment Period for the Detection 11: 43-50 of Salmonella enterica in Artificially Inoculated Meat Products Using Culture, PCR and LAMP Assays
Espiritu, C.L.A., Justo, C.A.C., Aptamer Selection Against a ACS Infectious Diseases Rubio, M.J., Svobodova, M., Trichomonas vaginalis Adhesion DOI 10.1021/acsinfecdis.8b00065 Bashammakh, A.S., Alyoubi, Protein for Diagnostic Applications A.O., Rivera W.L., Rollon, A.P. and O' Sullivan, C.K.
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cont. of Table 3. Adao, D.E.V. and Rivera, W.L. Recent Advances in Blastocystis sp. Philippine Science Letters Research. 11(1): 39-50
Salas, R.L., Garcia, J.K.D.L., Effects of Truncation of the Peptide Peptides Miranda, A.C.R., Rivera, W.L., Chain on the Secondary Structure and 104 : 7-14 Nella, R.B. and Sabido, P.M.G. Bioactivities of Palmitoylated Anoplin
Vital, P.G. , Zara, E.S., Paraoan, Antibiotic Resistance and Extended- Water C.E.M., Dimasupil, M.A.Z., Spectrum Beta-lactamase Production 10,548. DOI 10.3390/w10050548. Abello, J.J.M., Santos, I.T.G. of Escherichia coli Isolated from and Rivera, W.L. Irrigation Waters in selected Urban Farms in Metro Manila, Philippines
Valle, D.L., Jr., Cabal, A.B.S., Enteroaggregative Escherichia coli Southeast Asian Journal of Paclibare, P.A.P. and Rivera, Infections Among Children in a Tropical Medicine and Public W.L. Tertiary Hospital in the Philippines Health. 49 (3): 409-420.
Mathay, A.C.M.T.A., Dimasupil, Bacteriological Screening of Fresh and Philippine Science Letters M.A.Z., Rivera, W.L. and Vital, Fresh-cut Fruits Vended in Select 11(Supplement): 37-42. P.G. Open Air Open Air Markets and Supermarkets in Metro Manila, Philippines
Pedales, R.D.C. and Fontanilla, Forensic Entomology in the Philippine Journal of Science I.K.C. Philippines: Establishing Baseline 147(1): 17-25 Data on the Forensically Important Blow Fly Species Chrysomya megacephala (Fabricius, 1794)
Catap, E.S., Kho, M.J.I. and In vivo Nonspecific Journal of Ethnopharmacology Jimenez, M.R.R. Immunomodulatory and 215: 191-198 Antispasmodic Effects of Common Purslane (Portulaca oleracea Linn.) Leaf Extracts in ICR Mice
Anticamara, J.A. and Tan, Survival and Growth of Re-attached Science Diliman B.C.A. Storm-generated Coral Fragments 30:2, 5-31 Post Super-typhoon Haiyan (aka Yolanda)
Ramos, C.P.L. and Conato, M.T. Hydrothermal Synthesis, Crystal American Institute of Physics Structures, and Enantioselective Conference Proceedings Adsorption Property of Bis(L- 1958, 020017 histidinato)nickel(II) Monohydrate DOI: 10.1063/1.5034548
Pagalilauan, H.A.M., Paraoan Detection of Pathogenic Bioaerosols Archives of Environmental and C.E.M. and Vital, P.G. and Occupational Risk in a Philippine Occupational Health Landfill Site. 73(2): 107-114
Tayaban K.M.M., Pintor K.L. and Detection of Potential Harmful Algal Environment, Development and Vital P.G. Bloom-Causing Microalgae from Sustainability Freshwater Prawn Farms in Central 20: 1311-1328 Luzon, Philippines, for Bloom Monitoring and Prediction.
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cont. of Table 3. Denusta, P.J.T., Ayson, E.G.D., Gonad Development and Size-at J. Appl Ichthylol Laron, M.A., Aya, F.A. and Maturity of Silver Therapon 35: 933 – 943 Garcia, L.M.B. Leiopotherapon plumbeus (Kner DOI: 10.1111/JAI.13915 1864; Teleostei: Terapontidae) in Tropical Volcanic Lakes in South Luzon, Philippines
CHEMISTRY Buenaventura, A.G.E. and Yago, Chemiluminescence Detection of Philippine Journal of Science A.C.C. Chlorpyrifos via Luminol-H2O2 – 147(4): 763-773 Ferricyanide System Using Microcontroller-based Photometer
Buenaventura, A.G.E. and Yago, Facile Electrochemical Pretreatment AIP Conference Proceedings A.C.C. of Multiwalled Carbon Nanotube- 1958, 020029 Polydimethylsiloxane Paste Electrode doi: 10.1063/1.5034560 for Enhanced Detection of Dopamine View online: and Uric Acid http://doi.org/10.1063/1.5034560 American Institute of Physics
Atayde, Jr., E.C., Montalbo, Linear and Hyperbranched Materials Science Forum R.C.K. and Arco, S.D. Copolymers of PEG-Based Acrylates 940: 15-20 and Methacrylic Acid as pH- Responsive Hydrophobic Drug Carriers
Atayde, Jr., E.C., Montalbo, Temperature and pH Dependent Drug Philippine Journal of Science R.C.K. and Arco, S.D. Release of Block Copolymers of 147(3): 363-372 Metha-Crylic Acid ad Poly(Ethylene Glycol) Methyl Ether Methacrylates
Tiongson, J.K. A., Bruzon, Synthesis and Properties of Methoxy Journal of Chemical and D.A.V., Tapang, G.A. and and Nitrile Functionalized Engineering Data Martinez, I.S. Imidazolium 63, 1135-1145 Tris(pentafluoroethyl)trifluorophos- phate Ionic Liquids
Martin, Z.N.Z., Martinez, I.S. Surface Tension Data of n-Propane, n- Tellus B: and Nellas, R.B. Octane and n-Dodecane from 70, 1463807 Nucleation Simulations https://doi.org/10.1080/1600088 9.2018.1463807
NSRI (In-House) De Leon, M.P., Montecillo, A.D., Bacterial Diversity of Bat Guano from Plos One Pinili, D.S., Siringan, M.A.T. and Cabalyorisa Cave, Mabini, 1-17 Park, D.S. Pangasinan, Philippines: A First https://doi.org/10.1371/journal.p Report ofn the Metagenome of one.0200095 Philippine Bat Guano
De Leon, M.P., Park, A, Near-Complete Genome Sequences Microbiology Resource Montecillo, A.D., Siringan, of Streptomyces sp. Strains AC1-42T Announcements M.A.T., Rosan, A.R.R. and Kim, and AC1-42W, Isolated from Bat 7(7): 1-2, e00904-18 S.G. Guano from Cabalyorisa Cave, Mabini, Pangasinan, Philippines
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cont. of Table 3. Salvador, J.M., Apaga, D.L.T., Filipino DNA Variation at 12 X- Forensic Science International: Delfin, F.C., Calacal, G.C., chromosome Short Tandem Repeat Genetics Dennis, S.E. and De Ungria, Markers 36: e8-e12 M.C.A.
Calacal, G.C., Salvador, J.M., Pathology and DNA Analysis of Philippine Journal of Science Sagum, M.S., Fortun, R.D. and Exhumed Human Remains Three- 147(1): 9-16 De Ungria, M.C.A. years Post-mortem
De Ungria M.C.A. and Jose J. Validation of DNA Mixture Protocols: AFSN Forensic Asia M. How Many is Enough? 8:46-47.
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2. EXTENSION SERVICES
The NSRI’s second principal function is to render services to researchers. These services are the following:
(1) Technical assistance and advisory services (2) Training courses, seminars and conferences; and (3) Information and community services
The NSRI has, however, opened these services to institutions and agencies outside UP to maximize the use of facilities and equipment and to make the expertise of its research staff available to the general public.
2.1 TECHNICAL ASSISTANCE AND ADVISORY SERVICES
The NSRI research and technical staff provide technical and advisory services to researchers, students, government and private institutions as well as private individuals.
Technical Assistance
The NSRI’s technical services laboratories are organized into the following units:
A. Biological Research and Services Laboratory (BRSL) B. DNA Analysis Laboratory (DAL) C. Microbiological Research and Services Laboratory (MRSL) D. Research and Analytical Services Laboratory (RASL)
The services rendered by each laboratory are found in the succeeding pages. Socialized fees are charged by the laboratories to cover cost of supplies, equipment maintenance, utilities and services.
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BIOLOGICAL RESEARCH AND SERVICES LABORATORY (BRSL)
The Biological Research and Services Laboratory (BRSL) is one of the four research and technical laboratories of the UP Natural Sciences Research Institute (NSRI). It was established in 1999 from the then Electron Microscopy Room of NSRI that in turn evolved from the previous Biological Services Laboratory. The laboratory was created to effectively provide services related to microscopic analysis of biological samples. Since then, the laboratory has expanded its research and services thrust. It has undertaken researches in the fields of medical genetics, molecular biology, biotechnology, environmental genotoxicity, and DNA analysis of bioarchaeological samples. It also provides biological services adapted to specific needs of research in the natural sciences primarily in the evaluation of toxicity and safety of foods, drugs, cosmetics and other substances. BRSL’s facility is ideal for conducting experiments by researchers and students of UP and other universities and science personnel from government and industry. It has microscopy, cell culture, and molecular biology facilities.
In 2007, the BRSL pioneered the holding of a regular workshop at the NSRI on the proper care and use of laboratory animals in compliance with the Department of Agriculture Administrative Order No. 40 (AO 40) otherwise known as the Rules and Regulations on the Conduct of Scientific Procedures Using Animals. This has benefited many students and researchers in the country who lacked basic training in animal husbandry and laboratory animal
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procedures. The workshop is an essential component of the Animal Care and Use Program (ACUP) of UP Diliman. Moreover, as a response to the growing need on the use of laboratory animals in research and to better serve its clients in the scientific community, construction of an animal resource facility was started in 2011 with a grant received from the National Science Complex of the UP Diliman College of Science.
In 2018, the molecular biology workshop was revived with modified activities and up-to- date methods. With the title, “Basic molecular biology techniques for teachers and researchers,” this workshop equipped the participants with molecular biology concepts that they can use in their laboratories, universities, and industries.
Research
1) Title of Project : Genotoxicity and Antigenotoxicity Evaluation of Bark Extracts of Muntingia calabura L.
Project Leader : Ms. Raquel O. Rubio Research Associate : Jude Christian Francisco Funding Agency : OVCRD Duration of project : September 1, 2017 – April 2019 (extension)
2) Title of Project : Nutrient Depletion and Microbial Co-culture of Chlorella vulgaris for Bioethanol Production
Project Leader : Dr. Pierangeli G. Vital Graduate assistant : Katherine L. Pintor Funding Agency : NSRI - PCIEERD Duration of project : August 2017 – December 2018
3) Title of Project : Isolation, Identification, and Screening of Citric Acid- producing Fungal Endophytes from Citrus microcarpa
Project Leader : Dr. Pierangeli G. Vital Research Associate : Enrico S. Zara Funding Agency : NSRI Duration of project : January 1, 2018 – December 31, 2018
4) Title of Project : Philippine Environmental (Airborne and Surface) Germ Study in Primary Schools and Homes
Project Leaders : Dr. Pierangeli G. Vital and Dr. Windell L. Rivera Research Associates : Aurora Ines Aguilar and Samantha Valledor Funding Agency : P&G Singapore Duration of project : September 1, 2017 – December 31, 2018
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5) Title of Project : Assessment of Microorganisms from Commercially Available Probiotic Products Suitable for Children being Marketed in the Philippines and South Korea
Project Leader : Dr. Pierangeli G. Vital Graduate student : Clarizza May Dioso Funding Agency : OVCRD Duration of project : April 1, 2018 – April 1, 2019
6) Title of Project : Field Integrated Novel Diagnostics for flaviviruses (FIND FLAVIVIRUSES)
Project Leader : Dr. Raul V. Destura Co-investigator : Joy Ann P. Santos, MSc Graduate student : Adrian Abarientos Funding Agency : PCHRD-DOST Duration of project : January 15, 2018 – January 14, 2021
7) Title of Project : Development of Low Cost Point of Care Diagnostics for Simultaneous Detection of Paragonisimus westermani and Mycobacterium tuberculosis Using RPA Technology
Project Leader : Angelo dela Tonga, MSc Co-investigator : Joy Ann P. Santos, MSc Research associates : Stessi Marie Geganzo and Melanie Salinas Funding Agency : PCHRD-DOST Duration of project : July 1, 2018 – June 30, 2019
8) Title of Project : Evaluation of Indigenous Microalga as a Potential Lipid Producer for Biofuel Application (approved in 2018)
Project Leader : Dr. Pierangeli G. Vital Research Associates : Jessa DG. Carino Research Associates : NSRI Duration of project : January 1, 2019 – December 31, 2019
9) Title of Project : Basic Molecular Biology Techniques for Teachers and Researchers
Project Leaders : Dr. Pierangeli G. Vital and Joy Ann P. Santos, MSc Funding Agency : OEC OVCRD Duration of project : December 2018 – December 2019
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Extension Services/Clients
The BRSL continues to support the research and undergraduate/graduate programs of the University particularly those of the College of Science. Other clients include academic institutions, government agencies, and private companies. We provide bioassay and microscopy services, research assistance, and use of equipment, as well as conduct workshops on laboratory animal care and handling and basic molecular biology techniques. Data on the BRSL’s services are presented below.
BRSL Services in 2018 BRSL Services in 2018
(by % of clients) (by % of earnings)
2% 1% 0.3%
10% 56% 0.1% 38% 0.9% 20% 1.7%
0.6% 0.1% Consultation 42% 29% Equipment Use Workshops Bioassays (a) Microscopy (b) Research Assistance Others
Fig. 1. Charts of BRSL’s services in 2018 showing the % of clients that availed them (a), and the % earnings derived from those services (b). The charts show that while majority of clients were those that consulted and sought advice regarding their research, the lab did not earn from this service because the consultations were free. About 98% of the lab’s earnings were from services that only 30% of clients, combined, availed – workshops and bioassays. “Other” services include room use and sale of supplies to lab and equipment users.
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BRSL Clients in 2018
All Services Others Client type 3% I II III IV V VI 7% 1% Research…
Microscopy 16%
Bioassays 64% Workshops 9% Equipment Use
Consultation
0 20 40 60 80 100 120 140 160 # of clients
(a) (b) Fig. 2. The charts of services according to client type show that about 92% of clients belonged to the academe (Types I-IV) with the majority of them (64%) being non-UP researchers and students (IV). While most of the Type IV clients consulted regarding their theses and projects, they also availed of the lab’s other services such as bioassay services, equipment rental, microscopy, research assistance, as well as attended the BRSL’s training courses. Most of the Type I clients (students from UP Diliman College of Science) were participants of the Laboratory Animal Workshop, but some also used lab equipment, and availed of the microscopy service. NSRI- based researchers (Type II) mainly used the lab’s equipment. Clients from other departments or other UP units (Type III), came for consultation, submitted samples for bioassays (cytotoxicity and anti-genotixicity testing), and attended the workshops. Among the services availed by clients from private companies and government agencies (Type V) were the consultation, bioassay – specifically cytotoxicity testing –, and training. The few clients from multinational companies (Type VI) mainly attended the workshops, specifically the Molecular Biology Techniques Workshop.
BRSL Workshop Participants in 2018 9%
12% Client type Basic Molecular I II III IV V VI Techniques Workshop 43% 30% Laboratory Animal Workshop 6% 0 20 40 60 80 # of clients
(a) (b)
Fig. 3. About 20% of those who availed of the BRSL’s services in 2018 were workshop participants. (a) Majority of the participants attended the Laboratory Animal Workshop. They were mostly from the academe, with only several coming from government agencies. On the other hand, participants of the Basic Molecular Techniques Workshop, which was designed for research professionals, were chiefly from government agencies, and private and multinational companies. (b) Most of the participants from both workshops (79%), were from the academe (Types I-IV), and the majority of them (43%) were students from the College of Science, with non-UP researchers and students (Type IV) also comprising a large portion (30%). NSRI-based researchers did not attend any of the lab’s workshops in 2018.
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Support to Students
Student assistance
High school
February 7, 2018
Title : Alternative to Nonrenewable Fuel, Algae as Biofuel
Name of students : Noriel Sumayo, Clarice Cerda, Bettina Bañares, Rosemarie Lucio Adviser : Ms. Vivian Tan School : St. Paul College, Pasig
May 8-10, 2018
Title : Alpha-glucosidase Inhibition and Alpha-amylase Activity of Carica papaya L. for Type II Diabetes mellitus
Name of students : Madeleine Y. Elpa, Alyssa Emily L. Grumo Adviser : Mr. Zaldy D. Alima School : DepEd Jacinto P. Elpa National High School
June 5, 2018
Title : Protective Properties of Eggplant (Solanum melongena) Leaves Extract Against Oxidative Stress
Name of students : Jeanine Dizon, Isabela Evangelista, Matthew Basilio Adviser : Ms. Elizabeth Canlas School : Pampanga High School
June 18, 2018
Title : Decreasing Neurodegeneration Using Okra (Abelmoschus esculentus) Seed Extract in Zebrafish (Danio rerio) Embryos
Name of students : Julienne Moreno, Franxynne Limson, Therese Reyes Adviser : Elizabeth Canlas School : Pampanga High School
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a b
Fig. 4. High school students from (a) Pampanga High School and (b) San Miguel National High School use BRSL’s facilities to perform experiments.
Undergraduate
February 8, 2018
Title : Isolation and Identification of Endophytic Fungi in Vanda sanderiana (Orchidaceae) (Waling-waling)
Name of students : Claire R. Mendoza, Reabelle M. Javier Adviser : Dr. Lourdes V. Alvarez School : Polytechnic University of the Philippines
Graduate
2018, MS thesis (graduated)
Title : Interaction Between Microalgae and Phycospheric Bacteria for Potential Biodiesel Application
Name of student : Katherine L. Pintor Co-Advisers : Dr. Pierangeli G. Vital and Dr. Windell L. Rivera School : University of the Philippines Diliman
2018, MS thesis (ongoing)
Title : Evaluation of Commercially Available Probiotic Products Suitable for Children Marketed in Philippines and South Korea
Name of student : Clarizza May Dioso Co-Advisers : Dr. Pierangeli G. Vital and Dr. Windell L. Rivera School : University of the Philippines Diliman
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2018, MS thesis (ongoing)
Title : Optimization of Organic Acid Production by Filamentous Fungal Endophytes through Probabilistic Modeling
Name of student : Jude Christian Francisco Co-Advisers : Dr. Pierangeli G. Vital and Windell L. Rivera School : University of the Philippines
2018, MS thesis (ongoing)
Title : Phylogenetic Grouping and Carbapenemase Production of Escherichia coli from Agricultural Samples in Metro Manila, Philippines
Name of student : Enrico Zara Co-Advisers : Dr. Pierangeli G. Vital and Dr. Windell L. Rivera School : University of the Philippines Diliman
Analyses for thesis/special projects
Cytotoxicity assay – 32/37 (UP Diliman Department of Chemical Engineering; Antipolo City Science High School; Ateneo de Manila University; Caloocan City Science High School; Culiat High School; Don Mariano Marcos Memorial State University; Isabela State University; Lyceum of the Philippines University – Batangas; Manila Central University; Mindanao State University – Iligan Institute of Technology; Our Lady of Fatima University – Pampanga; School of the Holy Spirit of QC; Vinzons Pilot High School; University of Rizal System – Morong)
Genotoxicity/Anti-genotoxicity assay – 1/2 (San Roque National High School)
Linkages
Animal Health and Welfare Division, Bureau of Animal Industry Handong Global University, South Korea Mahidol University, Bangkok Manila HealthTek, Inc. Procter & Gamble, Singapore Unilab, Inc. UP Diliman Archaeological Studies Program UP Diliman Institute of Biology UP Diliman Institute of Environmental Science and Management UP Diliman Institutional Animal Care and Use Committee UP Los Baños College of Veterinary Medicine Institute of Molecular Biology and Biotechnology, National Institutes of Health UP Manila
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Trainings/Seminars conducted
1) Title of Training : Laboratory Animal Workshop
Funding Source : Participants paid a fee for lecture and laboratory Date : April 27, 2018 Trainors : Dr. Maria Amelita C. Estacio, Dr. Oscar Cabanayan, Dr. Rohani B. Cena, Dr. Ma. Josefa R. Pante, Raquel Rubio, Erna Louise Elias, Leonora Nudo, Czarina Catherine Arbis Fees : P 5,000.00 outsider participants, P 4,000.00 UP student/employee No. of participants : 16 Profile of participants : students, professionals
Fig. 5. Participants practice handling and restraining laboratory mice during the Laboratory Animal Workshop on April 27, 2018.
2) Title of Training : Laboratory Animal Workshop
Funding Source : Participants paid a fee for lecture and laboratory Date : June 8, 2018 Trainors : Dr. Maria Amelita C. Estacio, Raquel Rubio, Leonora Nudo and Jobi Subosa Fees : P 4,000.00 UP student No. of participants : 17 Profile of participants : UP Diliman Institute of Biology students and technical staff
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Fig. 6. UPD Institute of Biology undergraduate students and staff listen to the lecture of Dr. MAC Estacio during the Laboratory Animal Workshop on June 8, 2018.
3) Title of Training : Laboratory Animal Workshop
Funding Source : Participants paid a fee for lecture and laboratory Date : October 13, 2018 Trainors : Dr. Maria Amelita C. Estacio, Raquel Rubio, Erna Louise Elias and Leonora Nudo Fees : P 4,000.00/participant No. of participants : 15 Profile of participants : 13 students, 2 teachers from Philippine Science High School- Main Campus
Fig. 7. Participants from the Philippine Science High School Main Campus look on as trainor Dr. MAC Estacio demonstrates blood collection from the retro-orbital sinus of the mouse during the Laboratory Animal Workshop on October 13, 2018.
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4) Title of Training : Laboratory Animal Workshop
Funding Source : Participants paid a fee for lecture and laboratory Date : October 29, 2018
Trainors Dr. Maria Amelita C. Estacio, Dr. Ma. Josefa R. Pante, Dr. Daphne L. Jorca, Angelo Dela Tonga, Raquel Rubio, Erna Louise Elias, Leonora Nudo, Jude Christian Francisco, Aurora Ines Aguilar and Jobi Subosa Fees : P 5,000.00 outsider participant, P4,000.00 UP student/employee No. of participants : 21 Profile of participants : 18 UPD College of Science students and 3 professionals
Fig. 8. Participants listen to a lecture by speaker Angelo Dela Tonga on the biosafety risks in animal research during the Laboratory Animal Workshop on October 29, 2018.
5) Title of Training : Basic Molecular Techniques Workshop for Research Professionals
Funding Source : OVCRD Extension Grant; participants paid a fee Date : December 4-5, 2018 Trainors : Ms. Joy Ann P. Santos, Dr. Pierangeli G. Vital, Mr. Arnel Sicam, Ms. Aurora Ines Aguilar, Mr. Jude Christian Francisco, Ms. Samantha Joy Valledor and Mr. Enrico Zara Fees : P 12,000.00/participant No. of participants : 17 Profile of participants : professionals
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Fig. 9. NSRI Director Dr. Windell Rivera and BRSL Head Dr. Pierangeli Vital with the participants and facilitators during the Basic Molecular Techniques Workshop on December 4-5, 2018.
Manpower Development
Activities of BRSL Staff in 2018
1) PIERANGELI G. VITAL, PhD (University Researcher III) [starting August 1, 2018]
Positions held
Head, BRSL Early Career Ambassador of the Philippines, International Society for Microbial Ecology Corresponding Secretary, Phi Sigma Biological Sciences Honor Society, Alpha Chi Chapter Treasurer, Philippine Phycological Society, Inc.
Professional/Extension activities
Organizer/Trainer, BRSL Basic Molecular Biology Workshop Technical Working Group member for food safety standards in the Philippines, Department of Agriculture – Bureau of Agricultural Research
Seminars/Symposia/Trainings attended
Vital PG. Microbial Hazards of Philippine Produce: Health vs Food Safety? 32nd Anniversary of Institute of Biology, IB Auditorium, University of the Philippines Diliman. January 29, 2018. National Research Council of the Philippines Annual Scientific Conference and 85th General Membership Assembly. NRCP @ 85: Leading Frontier Research Policy for Legislation, Philippine International Convention Center. March 14, 2018. Speaker, Oral Presenter. Vital PG. Outside-to-inside: Investigating the Microbiology of Environmental Samples, Philippine Society for Microbiology, Inc. Cluster 2 Symposium, University of Santo Tomas, Manila. March 24, 2018.
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Poster Presented
Pintor KL, Meetam M, Vital PG. Accumulation of Total Lipids and Triacylglycerides as Biodiesel Precursors Upon Co-culturing a Phycospheric Bacterium with Indigenous Chlorella vulgaris, Philippine Society for Microbiology, Inc. Annual Convention and Scientific Meeting Legazpi City, Albay. July 19-20, 2018. Carascal MB, Bertuso ALG, Ingalla PC, Malajacan GT, Joson IV SE, Francisco JCE, Vital PG. Identification and Screening for Mycotoxin and Plant Growth Promoting Factor Production of Fungal Endophytes from Citrus microcarpa Using Genome Analysis, Philippine Society for Microbiology, Inc. Annual Convention and Scientific Meeting, Legazpi City, Albay. July 19-20, 2018. PG Vital and WL Rivera. Multilocus Sequence Typing or Escherichia coli from Various Agricultural Sources, International Society for Microbial Ecology. August 12 – 17, 2018.
Publications
Vital PG, Rivera WL, Abello JJM, Francisco JCE. Microbiological Assessment of Fresh, Minimally Processed Vegetables from Open Air Markets and Supermarkets in Luzon, Philippines, for Food Safety. Environment, Development and Sustainability. DOI 10.1007/s10668-017-0022-x. In press. Vital PG, Zara ES, Paraoan CEM, Dimasupil MAZ, Abello JJM, Santos ITG, Rivera WL. 2018. Anti- biotic Resistance and Extended- spectrum Beta- lactamase Production of Escherichia coli Isolated from Irrigation Waters from Selected Urban farms in Metro Manila, Philippines. Water. 10(548): 1-11. Mathay ACMTA, Dimasupil MAZ, Rivera WL, Vital PG. 2018. Bacteriological Screening of Fresh and Fresh-cut Fruits Vended in Select Open Air Markets and Supermarkets in Metro Manila, Philippines. Philippine Science Letters (Special Issue). 11 (Supplement): 37-42. Pagalilauan HAM, Paraoan CEM, Vital PG. 2018. Detection of Pathogenic Bioaerosols and Occupational risk in a Philippine Landfill Site. Archives of Environmental and Occupational Health. 73(2): 107-114. Tayaban KMM, Pintor KL, Vital PG. 2018. Detection of Potential Harmful Algal Bloom-causing Microalgae from Freshwater Prawn Farms in Central Luzon, Philippines, for Bloom Monitoring and Prediction. Environment, Development and Sustainability. 20: 1311-1328.
Award
Young Ambassador of the Year Award, International Society for Microbial Ecology
2) RAQUEL O. RUBIO (University Researcher II)
Positions held
Head, BRSL (until July 2018) Information Officer Board Member, Philippine Association for Laboratory Animal Science
Professional/Extension activities
Member, UP Diliman Institutional Animal Care and Use Committee Organizer/Trainer, BRSL Laboratory Animal Workshops Member, UP Diliman REPS General Assembly Ad Hoc Committee Member, UP Systemwide REPS Conference Support Committee Organizer, Philippine Environmental Mutagen Society Annual Convention Speaker/Trainer, Training on Animal Care and Use for Research. Matias H. Aznar Memorial College of Medicine, Inc., Cebu City. December 7-8, 2018.
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Publication
Amor, E.C., Catap, E.S., Estacio, M.A.C., Pante, M.J.R., Saloma, C.P., and Rubio, R.O. 2018. The UPD IACUC Handbook on the Care and Use of Laboratory Animals. Institutional Animal Care and Use, Office of the Vice Chancellor for Research and Development, University of the Philippines Diliman, Quezon City. In press.
Seminars/Conferences/Symposia/Trainings attended
NSRI Lecture Series: "The Intriguing SPIRALS: Links to Food Safety and Gut Health”. NSRI, UP Diliman. January 29, 2018. NSRI Lecture Series: “The Overview on Procurement Modes and Introduction of New Process and Template for Small Value Procurement.” NSRI, UP Diliman. April 17, 2018. The Philippine Association for Laboratory Animal Science (PALAS) 30th Annual Scientific Conference. Crowne Plaza Hotel Manila. May 24-25, 2018. The Philippine Genome Center/Natural Sciences Research Institute Seminar: Establishment and Management of BRCs/Biobanks: A Case of KNRRC. National Institute of Molecular Biology and Biotechnology, UP Diliman. July 17, 2018. UP Diliman REPS General Assembly. NISMED, UP Diliman. September 25, 2018. UP Systemwide REPS Conference. School of Statistics/NISMED, UP Diliman. October 18-19, 2018. World Experts Lecture Series: "Protozoan Interactions Influence Abundance of Genes Encoding Heavy Metal Resistance in the Environment." Institute of Biology, UP Diliman. November 12, 2018. 38th Annual Convention of the Philippine Environmental Mutagen Society. College of Science Auditorium, UP Diliman. November 24, 2018.
3) JOY ANN P. SANTOS (University Researcher I) [starting Sept. 1, 2018]
Professional/Extension activities
Organizer/Trainer. BRSL Basic Molecular Biology Workshop Speaker. Training on Nucleic Acid Amplification Test -- Loop-Mediated Isothermal Amplification (NAAT-LAMP). Mariano Marcos State University, Ilocos Norte. October 17-19, 2018. Speaker. Training on Nucleic Acid Amplification Test -- Loop-Mediated Isothermal Amplification (NAAT-LAMP). Research Institute for Tropical Medicine, Alabang. November 13-14, 2018.
Seminars/Symposia/Trainings attended
National Research Council of the Philippines Annual Scientific Conference and 85th General Membership Assembly. NRCP @ 85: Leading Frontier Research Policy for Legislation, Philippine International Convention Center. March 14, 2018. “Safety and Security in the Workplace: A Primer on NSRI’s Safety and Security Rules and Guidelines.” NSRI, UP Diliman. September 17, 2018.
Publication
Belizario, VB Jr., Destura RV, Gabunada RR, Petronio-Santos JA, Amarillo ML, and De Veyra C. (2018) Evaluation of Fecal and Serological Tests for the Diagnosis of Schistosomiasis in Selected Near-Elimination and Endemic Areas in the Philipppines. Southeast Asian Journal of Tropical Medicine and Public Health
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4) ERNA LOUISE L. ELIAS (University Research Associate I)
Positions held
REPS Non-supervisory representative, NSRI AcPC
Professional/Extension activities
Organizer. NSRI Lecture Series Organizer/Trainor. Laboratory Animal Workshop
Seminars/Symposia/Trainings attended
NSRI Lecture Series: "The Intriguing SPIRALS: Links to Food Safety and Gut Health”. NSRI, UP Diliman. January 29, 2018. Institute of Biology 32nd Anniversary Symposium. IB, UP Diliman. January 29, 2018. (no certificate) NSRI Lecture Series: "Sexual Dimorphism During Development: Man vs. Woman in a Molecular Level." NSRI, UP Diliman. February 28, 2018. NSRI Lecture Series: "Finding info @ www.cslib.upd.edu.ph." NSRI, UP Diliman. March 19, 2018. Data Privacy Act of 2012 Seminar-Workshop. College of Science Auditorium, UP Diliman. May 18, 2018. The Philippine Association for Laboratory Animal Science (PALAS) 30th Annual Scientific Conference. Crowne Plaza Hotel Manila. May 24-25, 2018. UP System-wide REPS Conference. School of Statistics/NISMED, UP Diliman. October 18-19, 2018. NSRI Lecture Series: "Probabilistic Graphical Models: Framework and Applications." NSRI, UP Diliman. October 18, 2018. World Experts Lecture Series: "Protozoan Interactions Influence Abundance of Genes Encoding Heavy Metal Resistance in the Environment." Institute of Biology, UP Diliman. November 12, 2018. World Experts Lecture Series: “Strains of Mucilaginibacter Adapt to Heavy Metal Stress by Acquisition of Genomic Islands." NSRI, UP Diliman. November 13, 2018. World Experts Lecture Series: "Phytoremediation Using Plant Growth Promoting Bacteria (PGPB)." Institute of Biology, UP Diliman. November 14, 2018. 38th Annual Convention of the Philippine Environmental Mutagen Society. College of Science Auditorium, UP Diliman. November 24, 2018.
5) MARIA GENESIS G. MAGLEO (University Research Associate I) [Jan 1 – Jun 30, 2018]
Professional/Extension activities
Support Staff. Laboratory Animal Workshop
Seminars/Symposia/Trainings attended
NSRI Lecture Series: "The Intriguing SPIRALS: Links to Food Safety and Gut Health”. NSRI, UP Diliman. January 29, 2018. Institute of Biology 32nd Anniversary Symposium. IB, UP Diliman. January 29, 2018. (no certificate) NSRI Lecture Series: "Sexual Dimorphism during Development: Man vs. Woman in a Molecular Level." NSRI, UP Diliman. February 28, 2018. NSRI Lecture Series: "Finding info @ www.cslib.upd.edu.ph." NSRI, UP Diliman. March 19, 2018. Data Privacy Act of 2012 Seminar-Workshop. College of Science Auditorium, UP Diliman. May 18, 2018. Anti-sexual Harassment Seminar. NSRI, UP Diliman. June 14, 2018.
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6) AURORA INES AGUILAR (University Research Associate I) [starting Aug 1, 2018]
Professional/Extension activities
Facilitator, Laboratory Animal Workshop, Biological Research and Services Laboratory (BRSL), NSRI, UP Diliman. October 28, 2018. Facilitator, Basic Molecular Biology Techniques Workshop, NSRI, UP Diliman, Diliman. December 4-5, 2018.
Seminars/Symposia/Trainings attended
“Safety and Security in the Workplace: A Primer on NSRI’s Safety and Security Rules and Guidelines.” NSRI, UP Diliman. September 17, 2018. Laboratory Animal Workshop. BRSL, NSRI, UP Diliman. October 13, 2018. NSRI Lecture Series: “Probabilistic Graphical Models Framework and Applications.” NSRI, UP Diliman. October 18, 2018. World Experts Lecture Series: “Protozoan Interactions Influence Abundance of Genes Encoding Heavy Metal Resistances in the Environment.” Institute of Biology, UP Diliman. November 12, 2018.
Publication
Calugay, R., Aguilar, A.I., Liwag, J., Ramos, P., & Giron, O. (2018). Robust cadmium removal and tolerance by a magnetotactic bacterium isolated from the sediment of an open-pit mine tailings lake. Philippine Science Letters, 11, pp. 13-17.
7) JUDE CHRISTIAN E. FRANCISCO (University Research Associate I) [starting Aug 1, 2018]
Professional/Extension activities
Facilitator, Laboratory Animal Workshop, Biological Research and Services Laboratory (BRSL), NSRI, UP Diliman. October 28, 2018. Facilitator, Basic Molecular Biology Techniques Workshop, NSRI, UP Diliman, Diliman. December 4-5, 2018.
Seminars/Symposia/Trainings attended
“Mushroom Production” (20th Annual Scientific Meeting, Symposium and Training), Institute of Biological Sciences, UP Los Baños. August 24, 2018. “Mycology and Global Change: Capitalizing on Fungal Research and Education in Building Resilient Communities.” 20th Annual Scientific Meeting, Symposium and Training. Institute of Biological Sciences, UP Los Baños. August 25, 2018. Laboratory Animal Workshop. BRSL, NSRI, UP Diliman. October 13, 2018. NSRI Lecture Series: “Probabilistic Graphical Models Framework and Applications.” NSRI, UP Diliman. October 18, 2018. World Experts Lecture Series: "Protozoan interactions influence abundance of genes encoding heavy metal resistance in the environment." Institute of Biology, UP Diliman. November 12, 2018. 38th Annual Convention of the Philippine Environmental Mutagen Society. ollege of Science Auditorium, UP Diliman. November 24, 2018.
Publication
Vital PG, Rivera WL, Abello JJM, Francisco JCE. Microbiological Assessment of Fresh, Minimally Processed Vegetables from Open Air Markets and Supermarkets in Luzon, Philippines, for Food Safety. Environment, Development and Sustainability. DOI 10.1007/s10668-017-0022-x.
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8) SAMANTHA JOY D. VALLEDOR (University Research Associate I) [Aug 1 – Dec 31, 2018]
Professional/Extension activities
Facilitator. Basic Molecular Biology Techniques Workshop, NSRI, UP Diliman, Diliman. December 4-5, 2018.
Seminars/Symposia/Trainings attended
“Gearing Up Towards a Globally Competitive, Accredited, and Certified Philippine Microbiology” PSM Cluster 2 Symposium, Buenaventura Garcia Paredes, O.P. Building College of Science, University of Santo Tomas, España Blvd., Sampaloc, Manila. March 24, 2018. “Multilocus Sequence Typing (MLTS) of Escherichia coli from Various Agricultural Sources for Food Quality Monitoring” by Dr. Pierangeli G. Vital, RDMIC Bldg., DA Complex, Elliptical Road, Quezon City. May 4, 2018. “Orientation to the University of the Philippines Anti-Sexual Harassment Code 2017”, Natural Sciences Research Institute, UP Diliman, Quezon City. June 13, 2018. “Safety and Security in the Workplace: A Primer on NSRI’s Safety and Security Rules and Guidelines”, Natural Sciences Research Institute, UP Diliman, Quezon City. September 17, 2018. “Probabilistic Graphical Models Framework and Applications” by Dr. Reinabelle C. Reyes, Natural Sciences Research Institute, UP Diliman, Quezon City. October 18, 2018. “Protozoan Interactions Influence Abundance of Genes Encoding Heavy Metal Resistances in the Environment” by Dr. Christopher G. Rensing, Institute of Biology, UP Diliman, Quezon City. November 12, 2018.
9) ENRICO ZARA (University Research Associate I) [Aug 1 – Dec 31, 2018]
Professional/Extension activities
Facilitator. Basic Molecular Biology Techniques Workshop, NSRI, UP Diliman, Diliman. December 4-5, 2018.
10) RYAN MAY T. JALOVA (Administrative Aide V) [starting Apr 1, 2018]
Seminars/Symposia/Trainings attended
Orientation on Procurement Policies and Guidelines- January 29, 2019 NSRI Laboratory Safety Seminar. September 17, 2018. Orientation to the University of the Philippines Anti- Sexual Harassment Code 2017. June 13, 2018. The Overview on Procurement Models and Introduction of New Process and Template for Small Value Procurement. April 17, 2018. Data Privacy Act of 2012 Seminar Workshop. May 18, 2018.
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The DNA Analysis Laboratory, NSRI-UPD is one of the four research and extension service laboratories at the Natural Sciences Research Institute of University of the Philippines, Diliman. The laboratory's research thrust includes the development of molecular tools to better understand the genetic variation in different Filipino regional and ethno-linguistic groups for forensic, health and molecular anthropological studies. The researchers and staff of DNA Analysis Laboratory, NSRI-UPD are committed to make use of excellent science, particularly in the field of molecular biology and genetics, in assisting the Philippines criminal justice system by “putting science at the service of society”.
Researches
On-going in 2018
Project Title Funding Agency Project leader Sama Origins and Transformations: Evidence Dr. Maria Corazon A. De from Genes, Languages, and Cultures funded CHED Ungria by the Commission on Higher Education The Reconstruction of Jose Rizal's DNA to study Dr. Maria Corazon A. De OVPAA-EFSTRG his Genealogy and Genetic Ancestry Ungria Palaeoenvironmental and Biodiversity Study of Main: Dr. Armand Mijares; Mindoro Island: An Archaeological Science OVPAA-EIRG Co: Dr. Maria Corazon A. Initiative – Project De Ungria
On-going collaborative research projects
Project Title Collaborative Agency Human evolutionary genetics study Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany Palaeoenviromental and Biodiversity Study of OVPAA-EMERGING INTERDISCIPLINARY RESEARCH Mindoro Island: An Archaeological Science GRANT: Initiative o Main proponent: Archaeological Studies Program o Co-proponents: . UPD-NIGS . UPD-Envi Sci . UPD-NSRI-DAL
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DNA-PROKIDS: Using DNA to fight childtrafficking Laboratory of Genetic Identification, University of Granada, Spain University of North Texas Health Science Center, USA Innocence Project Philippines Network: Using Free Legal Assistance Group (FLAG) DNA to help exonerate wrongfully convicted Office of Legal Aid, UP Diliman College of Law persons College of Law, De La Salle University College of Law, Ateneo De Davao
Extension Services/Clients
2018 DNA Testing Inquiries, n= 203 DNA services, n= 34
Support to Students
Mr. Frederick C. Delfin is working on his Doctoral Studies thesis under the International Max Planck Research School, Leipzig School of Human Origins, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany. Dr. Maria Corazon A. De Ungria teaches in the Science, Technology and Society Program (SPS) of the College of Science. Dr. Maria Corazon A. De Ungria teaches Bio397- Current Topics in Forensic Biology- at the Institute of Biology of the College of Science. Dr. Maria Corazon A. De Ungria is the co-adviser of Mr. Paul Ryan L. Sales for his Masteral Study in Biology under the Institute of Biology of the College of Science. Dr. Maria Corazon A. De Ungria is the co-adviser of Ms. Jazelyn M. Salvador for her Doctoral Study in Biology under the Institute of Biology of the College of Science.
Linkages
Local - Archaeological Studies Program (ASP) - Asia Against Child Trafficking (Asia-ACT) (NGO) - Child Protection-Network (CPU-Net) - Commission on Human Rights (CHR) - Department of Justice (DOJ) – Inter-Agency Council Against Trafficking (IACAT) - Department of Social Welfare and Development (DSWD) - National Academy of Science and Technology (NAST) - National Institutes of Health (NIH) - National Bureau of Investigation (NBI) - Outstanding Young Scientists Inc (OYS Inc) - Philippine Science High School System (PSHS) - The Outstanding Women in the Nation Service (TOWNS)
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International
- Academy of Science for the Developing World (TWAS) - Asian Forensic Science Network (AFSN) - DNA-Prokids - Max Planck Institute for Evolutionary Anthropology (Germany) - University of Granada (Spain) - University of North Texas Health Science Center (USA) - North Carolina State University (USA)
Visitors/Guests
Date of Visit Institute/Agency/School No. of attendees
14-Feb-18 Dr. Ricky Nolasco (CSSP, UP Diliman) 1
7-Mar-18 Dr. Francisco Datar & anthropology students 16
12-Mar-18 Marie Antoinette Villaflor (CS Library) 1
26-Jul-18 Atty. Paul Balite & law students 7
26-Sep-18 PSHS - Central Luzon Campus 6
24-Oct-18 PUP Manila 12
25-Oct-18 PUP Manila 13
26-Oct-18 FLAG 16
28-Nov-18 PUP Manila 33
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DAL’s Photo Gallery
Fig. 10. The Max-Planck Independent Research Group on Population Genomics, CAS- MPG Partner Institute for Computational Biology (PICB-PGG) and the DNA analysis Laboratory, Natural Sciences Research Institute, University of the Philippines Diliman (DNA analysis Laboratory, NSRI-UPD) worked together in collecting and studying human samples for scientific research and to create and exchange associated data and images in accordance with relevant national and regional laws and regulations concerning scientific research, human subjects and biodiversity including laws relating to access to human genetic resources, associated benefit sharing, intellectual property and traditional knowledge. Dr. Maria Corazon A. De Ungria and Ms. Jazelyn M. Salvador with the research team of PICB-PGG in Shanghai, China.
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Fig. 11. Collaboration between Promega Corp. and the DNA Analysis Laboratory for a beta testing validation of a newly developed kit to improve current laboratory procedures especially in analyzing challenged casework samples. Results of the validation were presented at the International Symposium for Human Identification 2018 at Arizona, USA.
Fig. 12. Dr. Maria Corazon A. De Ungria during the 10th Asian Forensic Science Network Annual Symposium in Beijing, China, September 4-8 2018.
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Fig. 13. CHED-NCCA project is a collaboration between three universities, namely UP Diliman, UP Los Banos, and the MSU-Tawi-Tawi through its Sama Studies Center. Photo of the study team from UP at Los Baños and UP Diliman with Dr. Maria Corazon A. De Ungria (center).
Fig. 14. Striving for Excellence in order to help more patients. UPD-NSRI DNA Analysis Laboratory team with the Child Protection Network team of UPM
Fig. 15. Dialogue with NAST Scientists held at the University of Eastern Philippines, Catarman Northern Samar organized by the National Academy of Science and Technology. Teachers from a national high school attended the lecture series. Dr. Maria Corazon A. De Ungria, Head of the laboratory, gave a presentation on DNA technology and its application.
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Fig. 16. Spier Conference Center, Stellenbosch, South Africa. The Pink Team after discussing Issues of data ownership, governance over research and biobanking and the role of local & international ethic committees during the Global Forum on Bioethics in Research.
Fig. 17. Puerto Princesa, Palawan. PanAsia SNP Initiative- Philippine Team. Interconnecting the social and natural sciences in studying the genetics and culture of different Philippine populations united towards a common goal of contributing towards progress for all.
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Manpower Development
Activities of DAL Staff in 2018
1) MARIA CORAZON A. DE UNGRIA (University Researcher V / DOST SCS Scientist II)
Positions held
DNA Analysis Laboratory Head (Feb 1999 – present) Professorial Lecturer 3, Forensic Biology Seminar Series, Institute of Biology, College of Science, University of the Philippines (Nov 2012 – present) Professorial Lecturer 2, Science and Society Program, College of Science, University of the Philippines (June 2009 – May 2012 Level I; June 2012 – December 2018 Level II)
Awards/Accreditation received
Included as one of the two Filipino women in the “Profile of Women Scientists in Asia” by the Association of Academies and Societies of Sciences in Asia.
Grant from the Wellcome Trust to travel to Stellenbosch, South Africa and attend the Global Forum on Bioethics in Research (GRBR) with the theme “The Ethics of Data Sharing and Biobanking in Health Research” by the Association of Academies and Societies of Sciences in Asia. Study Grant from the Philippine Council for Industry, Energy and Emerging Technology and Development of the Department of Science and Technology to conduct collaborative research entitled “Genomic Studies on Philippine Negrito and Mangyan Populations” in the CAS-MPG Partner Institute for Computational Biology (PICB), Shanghai Institutes for Biological Sciences (SIBS) Chinese Academy of Sciences (April 5 to September 4).
Publications
De Ungria, M.C.A. 2018. Validation of DNA Mixture Protocols: How Many is Enough? AFSN Forensic Asia. 8:46-47. Salvador, J.M., Apaga, D.L.T., Delfin, F.C., Calacal, G.C., Dennis, S.E. and De Ungria, M.C.A. 2018. Filipino DNA Variation at 12 X-Chromosome Short Tandem Repeat Markers. Forensic Science International: Genetics (advance online publication). Calacal, G,C,, Salvador, J.M., Sagum, M.S., Fortun, R.dR. and De Ungria, M.C.A. 2018. Pathology and DNA Analysis of Exhumed Remains Three-years Post-mortem. Philippine Journal of Science.147(1):9-16.
Book Chapter/Contribution
De Ungria, M.C.A. and Jose, J.M. Forensic Science in Challenging Environments: The Philippine Experience. 2018. In “Tending Life”. pp 1-12. Edited by MSI Diokno. Free Legal Assistance Group, Manila, Philippines.
Research grants
Sama Origins and Transformations: Evidence from Genes, Languages, and Cultures funded by the Commission on Higher Education. Palaeoenvironmental and Biodiversity Study of Mindoro island: An Archaeological Science Initiative funded by the Office of the Vice President for Academic Affairs, University of the Philippines to the Archaeological Studies Program through the Emerging Interdisciplinary Research (EIDR) Program. Reconstruction of Jose Rizal’s DNA to Study his Genealogy and Genetic Ancestry using Lineage and Ancestry Informative Markers funded by the Office of the Vice President for Academic Affairs (OVPAA), UP System under the Program on Forensics and Ethnicity of the Philippine Genome Centre.
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Lectures
“Forensic Genetics in the Philippines: Human Identification in Challenging Times” during the International Conference of the Genetics Society of Korea organized by the Genetics Society of Korea. K Hotel, Seoul, South Korea. November 29-30, 2018. “REPS Contribution to Knowledge Generation in Forensics and Ethnicity” during the UP System- wide REPS conference, UP Diliman. October 18-19, 2018. “PanAsia SNP Initiative” during the 40th Annual Meeting of the Anthropological Association of the Philippines with the theme ‘Our Interconnected: Doing Anthropology in a Time of Ecological Crisis’. Palawan State University, Puerto Princesa, Palawan. November 9, 2018. “Life as an Experiment: The World as My Laboratory” National Academy of Science and Technology Symposium for the Youth, University of Eastern Philippines, Catarman, Northern Samar. September 18, 2018. “A Tale of Two Cases: The Use of DNA Evidence in the Philippines” National Academy of Science and Technology Meet the Scientists Lecture Series, University of Eastern Philippines, Catarman, Northern Samar. September 17, 2018. “Working as a DNA Forensic Scientist in the Philippines” during the National Science Technology Fair organized by the Department of Education, Tagaytay International Convention Centre, Tagaytay City, Philippines. February 6, 2018.
Technical consultations
Resource Person for the Indie film entitled “Bakas” prepared by students of the PSHS Central Luzon campus, September 2018. The film won the Viewer’s Choice Award during the 3rd Indie- Siyensiya Film Competition. Resource Person for the Summer Internship Program of Law Students through the Innocence Project Philippines Network. June to July 2018.
Conference proceedings
De Ungria, M.C.A. 2018. Ethics in Research. Seminar on ‘Reconstructing Sama Origins and Transformations through Genes, Languages and Cultures’, held at the Mindanao State University – Tawi-tawi College of Technology and Oceanography, Bongao Tawi- tawi, October 26, 2018. Gallardo, B.G., Apaga, D.L.T., De Ungria, M.C.A. and Calacal, G.C. 2018. Evaluation of Promega’s Bone Demineralization Buffer for Processing Degraded Femur Samples. 29th International Symposium on Human Identification (ISHI). Phoenix Convention Center, Arizona USA. September 24-27, 2018. De Ungria, M.C.A. and Jose, J.M. 2018. A Tale of Two Cases: The Use of DNA Evidence in the Philippines. 10th Human Annual Meeting and Symposium of the Asian Forensic Science Network with the theme New Methods, New Technologies, New Challenges, Beijing, China (oral presentation). September 4-8 , 2018). C Phillips, D McNevin, M Eduardoff, M de la Puente, D Power, C Xavier, A Heidegger, W Parson, K Kidd, C Santos, R Lagacé, S Wootton, S Akhteruzzaman, M Barash, MCA De Ungria, T Gross, M Hashiyada, J Henry, SR Hong, HY Lee, C Oz, E Peters, P Schneider, NFJA Soliven, N Tuitoga, S Olson, MV Lareu, and R Daniel. Human Identification Solutions Conference. “An ancestry-informative assay for the Asia Pacific region”. Rome, Italy. 3-4 May 2018.
Graduate Students
Salvador, J.M. 2018. Genome-wide Studies of Philippine Negritos. Doctor of Philosophy in Genetics. University of the Philippines, Diliman, Philippines. Co-adviser with J Quilang (Adviser).
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Research Fellowship
Conducted collaborative research with Dr Shuhua Xu at the Chinese Academy of Sciences- Max Planck Group Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Shanghai, China. April 5 to September 4, 2018.
Seminars/Workshops/Training attended
Regional Workshop on Science and Technology with the theme “Developments Relevant to the Biological Weapons Convention (BWC) for Asia” held in Seda Vertis Hotel, Quezon City. November 21-22, 2018. Global Forum on Bioethics in Research with the theme “The Ethics of Data-Sharing and Biobanking in Health Research” held in Hotel Spielberg, Stellenbosch South Africa. November 13- 14, 2018. Seminar on "Being and Becoming: Reflections on Ethnicity and Learning, The Australian and Philippine Experience” by Professor Mary Anne Bin-Salik. College of Science, UP Manila. October 12, 2018. Advance Training Workshop on “Continuing Ethics Education”. National Institutes of Health, UP Manila. October 23-25, 2018. Seminar on “How to Publish with Genome Biology” by Dr. Yixin Yao. Chinese Academy of Sciences- Max Planck Group Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Shanghai, China. May 17, 2018 Seminar on “Genetic Structure, Admixture History, and Ancestry of East Asian Populations” by Yuchen Wang, PhD candidate. Chinese Academy of Sciences- Max Planck Group Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Shanghai, China. May 9, 2018. Seminar on “Genetic Structure, Population Admixture and Natural Selection of Uyghur and Tajik populations in Xinjiang” by Qidi Feng, PhD candidate. Chinese Academy of Sciences- Max Planck Group Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Shanghai, China. May 9, 2018. Seminar on “Population Genomics Studies of Human Evolutionary History, Local Adaptation, and Database Construction” by Chao Zhang, PhD candidate. Chinese Academy of Sciences- Max Planck Group Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Shanghai, China. May 9, 2018. Seminar on “The Impact of Demographic History on Human Complex Traits” by Dr. Charleston Chang. Chinese Academy of Sciences- Max Planck Group Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Shanghai, China. April 27, 2018. Seminar on “The Genomic and Cultural Landscape of Madagascar” by Dr. Mark Stoneking. Chinese Academy of Sciences- Max Planck Group Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Shanghai, China. April 16,2018. Seminar on "Finding Info @www.cslib.upd.edu.ph" by Ms. Marie Antoinette D. Villaflor. Natural Sciences Research Institute, UP Diliman, Quezon City. March 19, 2018.
Organizations
International
American Academy of Forensic Sciences - Associate Member (2016- present) International Society for Forensic Genetics - Member (2003-2004; 2015 to present) Quality Assurance & Standards Committee of the Asia Forensic Science Network– 2nd Secretary (2011- 2016) DNA Workgroup of the Asia Forensic Science Network – Member (2009- present) Regional East Asia DNA Profiling Group (REAFD) - Member (2007-2009) Phi Kappa Phi Honor Society– Member (2011- 2017) The Academy of Science for the Developing World (TWAS) - Affiliate Fellow (2007-2011)
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American Association of Blood Banks- Member (2005-2011) Philippine-American Academy of Science and Engineering - Member (2005-present) Third World Organization of Women Scientists (TWOWS) - Member (1999- present)
Local
Innocence Project Philippines Network – Board Member (2012- present) Forensic Odontology Society of the Philippines - Honorary Fellow (2013- present) Outstanding Young Scientists Inc. – Board Member (Secretary 2008 - 2014; Public Relations Officer 2007 - 2008) Philippine Association of Career Scientists - Member, Board of Trustees (2013 - 2015) The Outstanding Women in the Nation Service (TOWNS) - Member (2007- present) Philippine Society for Biochemistry and Molecular Biology - Member (2002 - present) National Research Council of the Philippines (Division III) – Member (2003 - present); Associate Member (1999 - 2003)
2) GAYVELLINE C. CALACAL (University Researcher II / DOST SCS Scientist I)
Positions held
University Researcher III (June 2017 – present) University Researcher II (February 2010 – May 2017) University Research Associate (March 1997 – January 2010) National Research Council of the Philippines Member (March 2017 – present) NSRI Administrative and Personnel Committee Member Scientist I (DOST-SCS) (March 2013 – present) Program Committee Member (Forensics and Ethnicity), Philippine Genome Center (November 2011 – December 2017)
Publications
Salvador, J.M., Apaga, D.L.T., Delfin, F.C., Calacal, G.C., Dennis, S.E. and De Ungria, M.C.A. 2018. Filipino DNA Variation at 12 X-Chromosome Short Tandem Repeat Markers. Forensic Science International: Genetics 36: e8-e12. https://doi.org/10.1016/j.fsigen.2018.06.008. Calacal, G,C,, Salvador, J.M., Sagum, M.S., Fortun, R.dR. and De Ungria, M.C.A. 2018. Pathology and DNA Analysis of Exhumed Remains Three-years Post-mortem. Philippine Journal of Science.147(1):9-16.
Research grants
DNA Testing of Child Trafficking Cases funded by the DNA-Prokids Initiative, Granada, Spain. Validation of DNA Typing Procedures and Assessment of Genetic Relationships among Filipino Ethnical Language Groups. Commitment Project of the DNA Analysis Laboratory, Natural Sciences Research Institute, University of the Philippines Diliman.
Conference proceedings
Gallardo, B.G., Apaga, D.L.T., De Ungria, M.C.A. and Calacal, G.C. 2018. Evaluation of Promega’s Bone Demineralization Buffer for Processing Degraded Femur Samples. 29th International Symposium on Human Identification (ISHI). Phoenix Convention Center, Arizona USA. September 24-27, 2018.
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Lectures
Instructor, “Management of the Dead in Large Disasters for Police First Responders Workshop” Philippine National Police Training Service, National Headquarters, Camp BGen Rafael T Crame, Quezon City Philippines. November 13-15, 2018. Seminars/Workshops/Training attended
29th International Symposium on Human Identification (ISHI), Phoenix Convention Center, Arizona USA. September 2018. HITA Workshop: DNA As a Biometric: Developing Standards for Data Exchange, Phoenix Convention Center, Arizona USA. September 2018. AABB Accreditation Program Workshop, Phoenix Convention Center, Arizona USA. September 2018. Orientation to the University of the Philippines Anti-Sexual Harassment Code 2017. NSRI, University of the Philippines, Diliman, Quezon City, Philippines. June 2018. Data Privacy Act of 2012 Seminar-Workshop. College of Science, National Science Complex, University of the Philippines, Diliman, quezon City Philippines. May 2018. MiSeq FGx and ForenSeq Training. Philippine Genome Center. University of the Philippines, Diliman, Quezon City, Philippines. April 2018.
Organizations
International
International Society for Forensic Genetics – Member (2013-present) DNA Workgroup of the Asia Forensic Science Network - Member Representative (2010-present)
Local
National Research Council of the Philippines-Medical Sciences – Member (2016-present) Philippine Association of Career Scientist – Member (2013 - present) Outstanding Young Scientists Inc. – Member (2010 - present) Philippine Biosafety and Biosecurity Association, Inc (PhBBA) – Member (2009 - present) Philippine Society for Biochemistry and Molecular Biology (PSBMB) – Member (2002 - present) Philippine Society for Microbiology (PSM) – Member (2000 - present) Philippine Association of Medical Technologists (PAMET) – Member (1993 - present)
3) JAZELYN M. SALVADOR (University Research Associate I)
Positions held
University Researcher I (16 October 2018 - present) University Research Associate I (March 2002 – 15 October 2018) Project Leader, Philippine Population Reference Genetic Database at Twelve X-chromosomal Short Tandem Repeat (X-STR) DNA Markers for Forensic Applications (September 2016 – September 2017)
Awards/Accreditation received
International Publication Award given by the President of the University of the Philippines for the paper “Filipino DNA variation at 12 X-chromosome short tandem repeat markers”. Forensic Science International Genetics 36:e8-e12.
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Recipient of a Research Fellowship Grant from DOST-PCIEERD to conduct research on “Characterization of Genomic Diversity of Selected Philippine Negrito Groups” with the Max- Planck Independent Research Group on Population Genomics of CAS-MPG Partner Institute for Computational Biology. Shanghai, China. April 11 – August 10, 2018.
Publications
Salvador, J.M., Apaga, D.L.T., Delfin, F.C., Calacal, G.C., Dennis, S.E. and De Ungria, M.C.A. 2018. Filipino DNA Variation at 12 X-Chromosome Short Tandem Repeat Markers. Forensic Science International: Genetics 36: e8-e12. https://doi.org/10.1016/j.fsigen.2018.06.008. Calacal, G,C,, Salvador, J.M., Sagum, M.S., Fortun, R.dR. and De Ungria, M.C.A. 2018. Pathology and DNA Analysis of Exhumed Remains Three-years Post-mortem. Philippine Journal of Science.147(1):9-16.
Research grants
Validation of DNA Typing Procedures and Assessment of Genetic Relationships among Filipino Ethnical Language Groups. Commitment Project of the DNA Analysis Laboratory, Natural Sciences Research Institute, University of the Philippines Diliman.
Research Fellowship
Research Fellowship to conduct research on “Characterization of Genomic Diversity of Selected Philippine Negrito Groups” with the Max-Planck Independent Research Group on Population Genomics of CAS-MPG Partner Institute for Computational Biology, Shanghai, China. April 11 to 10 August 2018.
Seminars/Workshops/Training attended
40th UGAT (Ugnayang Pang-aghamtao) Annual Conference (An International Gathering). Palawan State University, Puerto Princesa City, Palawan. November 8-10, 2018. Seminar on “How to Publish with Genome Biology” by Dr. Yixin Yao. Chinese Academy of Sciences- Max Planck Group Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Shanghai, China. May 17, 2018 Seminar on “Genetic Structure, Admixture History, and Ancestry of East Asian Populations” by Yuchen Wang, PhD candidate. Chinese Academy of Sciences- Max Planck Group Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Shanghai, China. May 9, 2018. Seminar on “Genetic Structure, Population Admixture and Natural Selection of Uyghur and Tajik populations in Xinjiang” by Qidi Feng, PhD candidate. Chinese Academy of Sciences- Max Planck Group Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Shanghai, China. May 9, 2018. Seminar on “Population Genomics Studies of Human Evolutionary History, Local Adaptation, and Database Construction” by Chao Zhang, PhD candidate. Chinese Academy of Sciences- Max Planck Group Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Shanghai, China. May 9, 2018. Seminar on “The Impact of Demographic History on Human Complex Traits” by Dr. Charleston Chang. Chinese Academy of Sciences- Max Planck Group Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Shanghai, China. April 27, 2018. Seminar on “The Genomic and Cultural Landscape of Madagascar” by Dr. Mark Stoneking. Chinese Academy of Sciences- Max Planck Group Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Shanghai, China. April 16, 2018.
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Organizations
International
DNA Working Group of the Asian Forensic Science Network (AFSN). Member since 2009.
Local
Philippine Genome Center, Program on Forensics and Ethnicity Committee – Member since 2013- December 2017 Carl Duisberg Association of the Philippines (CDAP) Inc. – Member since 2009 and Board Member from 2012-2013 Network for Integrative Multidisciplinary Bioinformatics Utilization Strategies, Inc. (NIMBUS Inc.) – Life Member since 2009 Philippine Society for Microbiology (PSM) – Member since 2004 Philippine Network of Microbial Culture Collections (PNMCC) – Member 2009-2010 Philippine Society for Biochemistry and Molecular Biology (PSBMB) – Member since 2002
4) FREDERICK C. DELFIN (University Research Associate I)
Positions held
University research III (2018 to present) University Research Associate 1 (1998 – 2018) Member, Program on Forensics and Ethnicity, Philippine Genome Center, University of the Philippines (May 2011 – December 2017) Publication
Salvador, J.M., Apaga, D.L.T., Delfin, F.C., Calacal, G.C., Dennis, S.E. and De Ungria, M.C.A. 2018. Filipino DNA Variation at 12 X-Chromosome Short Tandem Repeat Markers. Forensic Science International: Genetics 36: e8-e12. https://doi.org/10.1016/j.fsigen.2018.06.008.
Research grants
Genetic diversity in Philippine populations, the peopling of the Philippines and the Asia-Pacific region funded by the International Max Planck Research School, Graduate Studies (Doctoral) Program, Leipzig School of Human Origins-Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany Department of Human Genetics, Leiden University Medical Center, Leiden University, Leiden, The Netherlands. “Validation of DNA Typing Procedures and Assessment of Genetic Relationships among Filipino Ethnical Language Groups” grant awarded by the Natural Sciences Research Institute, University of the Philippines, Diliman.
Technical consultations
Consultant. Commission on Higher Education. Discovery Applied Research and Extension for Trans/Interdisciplinary Opportunities (DARETO) Research program: “STOP Rabies: Synoptic Study on Transmission and Optimum Control to Prevent Rabies”.
Seminars/Workshops/Training attended
40th UGAT (Ugnayang Pang-aghamtao) Annual Conference (An International Gathering) with the theme “Our Interconnectedness: Doing Anthropology in a Time of Ecological Crisis), Palawan State University, Puerto Princesa City, Palawan. November 8–10, 2018
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Natural Sciences Research Institute (NSRI) Seminar. "Probabilistic Graphical Models Framework and Applications", NSRI Conference Room, Miranda Hall, University of the Philippines, Diliman, Quezon City. October 18, 2018. Training-Workshop on Health Research Ethics and Good Clinical Practice, Hotel Kimberly, Malate, Manila., August 24–24, 2018. Orientation to the University of the Philippines Anti-Sexual Harassment Code 2017, Natural Sciences Research Institute (NSRI) Conference Room, Miranda Hall, University of the Philippines, Diliman, Quezon City. June 13, 2018, 36th Anniversary, Philippine Council for Health Research and Development, Research on Social Innovations in Health, Philippine International Convention Center (PICC), Pasay City, Philippines. March 16, 2018.
Organizations
Local
Core Committee Member; Forensics and Ethnicity Program, Philippine Genome Center, University of the Philippines System, Diliman, Quezon City Philippines; 2013 to December 2017.
5) MIRIAM RUTH M. DALET (University Research Associate I)
Positions held
University Research Associate 1 (Feb 2002 – present) Board Member, Innocence Project Philippines Network, Inc.
Research grants
Reconstruction of Jose Rizal’s DNA to Study his Genealogy and Genetic Ancestry using Lineage and Ancestry Informative Markers funded by the Office of the Vice President for Academic Affairs, University of the Philippines under the Program on Forensics and Ethnicity of the Philippine Genome Center of the University of the Philippines. DNA Testing of Child Trafficking Cases funded by the DNA-Prokids Initiative, Granada, Spain. Validation of DNA Typing Procedures and Assessment of Genetic Relationships among Filipino Ethnical Language Groups. Commitment Project of the DNA Analysis Laboratory, Natural Sciences Research Institute, University of the Philippines Diliman.
Seminars/Workshops/Training attended
NSRI Seminar Series/World Experts Lecture Series: “Protozoan Interactions Influence Abundance of genes Encoding Heavy Metal Resistances in Environment.” Institute of Biology, College of Science, UP Diliman, Quezon City. November 12, 2018. REPS Conference 2018. School of Statistics Auditorium, UP Diliman, Quezon City. October 18-19, 2018. PGC Scientific Symposium: “Forging research collaborations, moving forward together.” Institute of Biology Auditorium, National Science Complex, UP Diliman, Quezon City. September 11, 2018. Center Law, UP Law Center and Commission on Human Rights Conference “Human Rights: Contemporaneous Strategies and Future Pathways, An Academic Conference on Human Rights Accountability.”Seda Vertis North Hotel, Quezon City. September 6, 2018. Forensic Technology Center of Excellence webinar “Providing Gender Inclusive Care to Victims of Sexual Assault.” August 30, 2018 (1hr, 15min). PGC and NSRI seminar “Establishment and Management of BRCs/Biobanks: A case of KNRRC.” National Institute of Molecular Biology And Biotechnology, UP Diliman, Quezon City. July 17, 2018. PGC seminar “Applications of PacBio Sequencing Technology.” National Institute of Molecular Biology And Biotechnology, UP Diliman, Quezon City. July 5, 2018.
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Office of Anti-Sexual Harassment seminar “Orientation to the University of the Philippines Anti- Sexual Harassment Code 2017.” Natural Sciences Research Institute, UP Diliman, Quezon City. June 13, 2018. UPD Protection Office and UP College of Science “Data Privacy of 2012 Seminar-Workshop.” College of Science Auditorium, CS New Administration Bldg., National Science Complex, UP Diliman, Quezon City. May 18, 2018. NSRI Seminar Series: “The Overview on procurement Modes and Introduction of New Process and template for Small Value Procurement.” Natural Sciences Research Institute, UP Diliman, Quezon City. April 17, 2018. Forensic Technology Center of Excellence webinar “Evaluate Population-Specific Sibship During Kinship Analysis.” March 21, 2018 (1hr, 15min). NSRI Seminar Series: “Finding Info @ www.cslib.edu.ph.” Natural Sciences Research Institute, UP Diliman, Quezon City. March 19, 2018. Forensic Technology Center of Excellence webinar “Cost-Benefit Analysis of Kinship Testing Involving Siblings.” March 7, 2018 (1hr, 15min).
Organizations
International
Regional East Asia Forensic DNA (REAFD) Group (2007 – 2010) Quality Assurance and Standards Committee of Asia Forensic Science Network (2010 – present)
6) MINERVA S. SAGUM (University Research Associate I)
Positions held
University Research Associate 1 (June 2006 – present) EU Project Research Associate (Oct 2002 – 2003) DNA Analysis Laboratory, NSRI-UPD Research Associate (2003 – May 2006) Board Member, Innocence Project Philippines Network, Inc. NSRI Special Events Committee Head (2013 - present)
Publications
Calacal, G,C,, Salvador, J.M., Sagum, M.S., Fortun, R.dR. and De Ungria, M.C.A. 2018. Pathology and DNA Analysis of Exhumed Remains Three-years Post-mortem. Philippine Journal of Science.147(1):9-16.
Research grants
DNA Testing of Child Trafficking Cases funded by the DNA-Prokids Initiative, Granada, Spain. Validation of DNA Typing Procedures and Assessment of Genetic Relationships among Filipino Ethnical Language Groups. Commitment Project of the DNA Analysis Laboratory, Natural Sciences Research Institute, University of the Philippines Diliman.
Seminars/Workshops/Training attended
World Lecture Series “Protozoan Interactions Influence Abundance of Genes Encoding Heavy Metal Resistances in the Environment”. Institute of Biology Auditorium, UP Diliman Quezon City. November 12, 2018. NSRI Lecture Series “Probabilistic Graphical Models Framework and Applications”. Natural Sciences Research Institute Conference Room, UP Diliman Quezon City. October 18, 2018. “Human Rights: Contemporaneous Strategies and Future Pathways an Academic Conference on Human Rights Accountability”. Seda Vertis North Hotel Quezon City. September 6, 2018.
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Office of Anti Sexual Harassment seminar “Orientation to the University of the Philippines Anti- Sexual Harassment Code 2017.” Natural Sciences Research Institute, UP Diliman, Quezon City. June 13, 2018. UPD Protection Office and UP College of Science “Data Privacy of 2012 Seminar-Workshop.” College of Science Auditorium, CS New Administration Bldg., National Science Complex, UP Diliman, Quezon City. May 18, 2018. 5th Forensic Science Symposium 2018: “ForenSeeks: Seeking Solutions through Forensics.”. Institute of Biology Auditorium, College of Science, UP Diliman, Quezon City. April 6 , 2018.
Organizations
International
Regional East Asia Forensic DNA (REAFD) Group. Member (2007-2009). Quality Assurance and Standards Committee of Asia Forensic Science Network – Member (2009 – present)
Local
Philippine Society for Microbiology (PSM) – Member Philippine Association for Teacher Education (PAFTE) – Associate Member
7) PAUL RYAN L. SALES (University Research Associate I)
Positions held
DAL Trainee (Feb 2012 – April 2012) Research Associate (May 2012 – February 2013) University Research Associate 1 (March 2013 – present)
Research grants
Validation of DNA Typing Procedures and Assessment of Genetic Relationships among Filipino Ethnical Language Groups. Commitment Project of the DNA Analysis Laboratory, Natural Sciences Research Institute, University of the Philippines Diliman.
Lectures
Guest Lecturer: Biology 1: Fundamentals of Forensic DNA Analysis. Institute of Biology, University of the Philippines, Diliman. March 23, 2018. Invited Speaker: Forensics: Mass Casualty Identification and Intervention. College of Arts and Sciences Theater, University of the Philippines Manila. July 26, 2018. Guest Lecturer: Anthropology 198: The Role of DNA in Mass Disaster Victim Identification. Institute of Biology, University of the Philippines, Diliman. October 16, 2018. Invited Speaker. Interactive Workshop on DNA Forensics of the 25th Biosciences Quiz Bee. NISMED Auditorium, University of the Philippines Diliman. October 13, 2018. Invited Speaker. Forensics: Unraveling Crime Scenes Through Science. College of Engineering and Architecture, Polytechnic University of the Philippines, Manila. October 19, 2018.
Seminars/Workshops/Training attended
MiSeq FGX and ForenSeq Training. National Institute of Molecular Biology and Biotechnology, University of the Philippines Diliman, Quezon City. April 24-26, 2018. Introductory Forensic Anthropology and Human Bone Identification (Short Course). Archaeological Studies Program, University of the Philippines Diliman, Quezon City. June 25-29, 2018.
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Anti-Sexual Harassment Code 2017.” Natural Sciences Research Institute, UP Diliman, Quezon City. June 13, 2018. UPD Protection Office and UP College of Science “Data Privacy of 2012 Seminar-Workshop.” College of Science Auditorium, CS New Administration Bldg., National Science Complex, UP Diliman, Quezon City. May 18, 2018. 5th Forensic Science Symposium 2018: “ForenSeeks: Seeking Solutions through Forensics.”. Institute of Biology Auditorium, College of Science, UP Diliman, Quezon City. April 6, 2018.
Organizations
International
DNA Working Group Asia Forensic Science Network – Member (2012- present)
Local
Philippine Environmental Mutagen Society – Member (2011-present) UP Pre-Medical Honor Society - Member (2008 – 2011)
8) NELVIE FATIMA JANE A. SOLIVEN (University Research Associate I)
Positions held
DAL Trainee (Oct 2014) University Research Associate 1 (Nov 2014 – present)
Research grants
Validation of DNA Typing Procedures and Assessment of Genetic Relationships among Filipino Ethnical Language Groups. Commitment Project of the DNA Analysis Laboratory, Natural Sciences Research Institute, University of the Philippines Diliman. DNA profiling of human urine samples for identification funded by the Office of the Vice Chancellor for Research and Development, University of the Philippines Diliman.
Conference proceedings
C Phillips, D McNevin, M Eduardoff, M de la Puente, D Power, C Xavier, A Heidegger, W Parson, K Kidd, C Santos, R Lagacé, S Wootton, S Akhteruzzaman, M Barash, MCA De Ungria, T Gross, M Hashiyada, J Henry, SR Hong, HY Lee, C Oz, E Peters, P Schneider, NFJA Soliven, N Tuitoga, S Olson, MV Lareu, and R Daniel. Human Identification Solutions Conference. “An ancestry-informative assay for the Asia Pacific region”. Rome, Italy. 3-4 May 2018.
Lectures
Forensics: Unraveling Crime Scenes Through Science. College of Engineering and Architecture, Polytechnic University of the Philippines, Manila. 19 October 2018.
Seminars/Workshops/Training attended
1st Annual College of Science Graduate Students’ Research Conference. Institute of Biology, University of the Philippines Diliman. October 22, 2018. Probabilistic Graphical Models Framework and Applications. Natural Sciences Research Institute, University of the Philippines Diliman, Quezon City. October 18, 2018. Philippine Genome Center Scientific Symposium: “Forging Research Collaborations, Moving Forward Together” Philippine Genome Center, University of the Philippines. September 11, 2018.
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Human Rights: Contemporary Strategies and Future Pathways, An Academic Conference on Human Rights Accountability. University of the Philippines College of Law Institute of Human Rights, Commission on Human Rights, Center for International Law. September 6, 2018. Introductory Forensic Anthropology and Human Bone Identification (Short Course). Archaeological Studies Program, University of the Philippines Diliman, Quezon City. June 25-29, 2018. MiSeq FGX and ForenSeq Training. National Institute of Molecular Biology and Biotechnology, University of the Philippines Diliman, Quezon City. April 24-26, 2018. “Sexual Dimorphism During Development: Man vs. Woman on a Molecular Level”. Dr. Lerrie Ann DG Ipulan-Colet. February 28, 2018.
Organizations
Local
Institute of Biology Alumni Association University of the Philippines Alumni Association Floorball Philippines
9) BEA G. GALLARDO (Laboratory Research Associate)
Positions held
University Research Associate 1 (January 2019 – present) Laboratory Research Associate (July 2016 -December 2018)
Conference Proceedings
Evaluation of Promega’s Bone Demineralization Buffer for Processing Degraded Femur Samples. BG Gallardo, DLT Apaga, MCA De Ungria, and GC Calacal. 29th International Symposium on Human Identification (ISHI). Phoenix, Arizona. September 24-27, 2018.
Seminars/Workshops/Training attended
Molecular Pathology. Philippine Society of Pathologists, INC, Diliman, Quezon City. November 15, 2018. World Experts Lecture Series: Protozoan Interactions Influence Abundance of Genes Encoding Heavy Metal Resistances in the Environment. Institute of Biology, UP Diliman Quezon City. November 12, 2018. NSRI Seminar Series: “Probabilistic graphical models framework and applications”. NSRI, UP Diliman, Quezon City. October 18, 2018. “Establishing a Culture Collection of Microorganisms and Preserved Parasites for Instruction and Research Purposes”. Philippine Association of Schools of Medical Technology and Public Health, Manila Central University, Caloocan City. September 8, 2018. Human Rights: Contemporaneous Strategies and Future Pathways. Seda Vertis North Hotel, Quezon City. September 6, 2018. 10Qs for Health Care Professional’s. Philippine Association of Schools of Medical Technology and Public Health, Manila Central University, Caloocan City. March 10, 2018.
Organizations
Local
Philippine Association of Medical Technologists (PAMET) – Member (2016 – present)
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MICROBIOLOGICAL RESEARCH AND SERVICES LABORATORY (MRSL)
Moving towards becoming a vital microbial resource center, the Microbiological Research and Services Laboratory (MRSL) has continuously carried out its mission, that is, to provide quality microbiological services and expertise to a wide range of clients. The laboratory has been engaged in developing human resources, strengthening science education, productive research collaboration, and supporting the endeavors and programs of the industry.
In ensuring high quality microbiological services, MRSL has strengthened its technical competency. Ms. Vina B. Argayosa, University Researcher II and a Specialist Microbiologist certified by the Philippine Academy of Microbiology (PAM) of the Philippine Society for Microbiology, Inc (PSM), has undergone training on “Microbiological Analysis of Water” at the National Reference Laboratory (NRL) under the Department of Health (DOH) in East Avenue Medical Center (EAMC), Diliman, Quezon City from August 7 to 10, 2018. The training oriented laboratory personnel of water testing laboratories in the Philippines on the Philippine National Standard for Drinking Water (PNSDW) and in the mandate of DOH relevant to PNSDW. It also equipped participants in the standards of operations for water testing laboratories. Participation in the said training is a requirement for the accreditation of laboratories for drinking water analysis by the Health Facilities and Services Regulatory Bureau (HFSRB) of DOH.
Moreover, Ms. Mary Ann Cielo R. San Diego has joined the technical staff of the MRSL as a UR I in September 2018. She has been undergoing training in the management and technical aspects of laboratory operations under the supervision of Dr. Maria Auxilia T. Siringan, MRSL Head. Ms. San Diego, who holds a MS degree in Microbiology, has successfully passed the PAM Certification Examination for Registered Microbiologist (RMicro) on December 1 to 2, 2018.
As the MRSL builds up its technical capability, it has also contributed to the development of human resources of different institutions through its short-term training courses. In 2018, the laboratory has organized and conducted the following training courses: a) Training on Bacteriological Examination of Water on February 19 to 23, 2018; b) Training Course on Microbiological Test Methods for the Sugar-Alcohol Industry on August 13 to 17, 2018; c) Customized Training on Microbiological Analysis of Water for a Water Testing Laboratory on September 10 to 14, 2018; and d) Training on Microbiological Analyses of Food: Quality Indicators and Gram-negative Bacteria on October 15 to 19, 2018. Through the training courses, concepts relevant to microbiological assessment of water and food were discussed and microbiological test methods were performed during the extensive hands-on laboratory exercises. The training courses have become a venue for developing technical expertise that supports the quality infrastructure of government regulatory agencies, private organizations and the manufacturing industry.
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The laboratory has continuously provided quality microbiological services and technical advice to students from secondary and higher educational institutions Nearly 57% (56.8%) of the services performed for non-water samples were provided to the students. Through the services, science-based data were generated, enabling the students to have better understanding of concepts and developing analytical thinking. MRSL was able to enrich scientific learnings as the laboratory assisted the students in the conceptualization and conduct of their investigatory projects, capstone researches and theses.
Dr. Siringan had productive research collaborations with promising researchers. Dr. Marie Christine M. Obusan, Assistant Professor of the Institute of Biology (IB), University of the Philippines-Diliman (UPD) with co-authors Dr. Siringan, Dr. Windell L. Rivera, Professor in IB and Dr. Lemnuel V. Aragones, Professor in the Institute of Environmental Science and Meteorology published an article on antibiotic susceptibility profiles of bacteria from stranded cetaceans in Aquatic Mammals. Dr. Marian P. De Leon, University Researcher II of the Museum of Natural History in UP-Los Baños (UPLB) together with Dr. Siringan and other co-authors published two scientific articles on the bacteria associated with bat guano from CabalyorisaCabe in Mabini, Pangasinan.
Dr. Siringan is currently undertaking a study, entitled, “Isolation of Plant-growth Promoting Bacteria Associated with the Soil-Rice Rhizosphere Ecosystem” under the project, “Microbial Diversity in the Long-term Fertility Experiment in Irrigated Lowland Rice Ecosystem (Microbes in Rice Ecosystem). The study aims to collect and preserve soil-rice rhizosphere- derived bacteria with ability to produce the plant growth-promoting hormone, indole acetic acid (IAA), nitrogen-fixing potential and phosphate solubilizing activity. The project is a collaborative research with Ms. Jayvee A. Cruz and Dr. Wilfredo B. Collado of the Philippine Rice Research Institute (PhilRice) in Muñoz, Nueva Ecija. Funding for the project was provided by the Department of Agriculture Region 3 (DA Region 3). In this project, diversity of microbial communities in soil samples collected from irrigated lowland rice ecosystem and in less intensive rice cropping systems at different stages of rice production, was determined using ecoplate analysis-based metabolic profiling. Metabolic profiling was generated using the GenIIIBiOLOG system in MRSL.
In March 2018, Dr. Siringan has completed the studies under the Phase I of the program, “Discovery and Development of Health Products: Marine Component (DDHP: Marine)”, headed by Dr. Arturo O. Lluisma of the Marine Science Institute, UP Diliman. Through the program, a marine microbial culture collection has been established under the University of the Philippines Culture Collection (UPCC) in MRSL. Cultures are maintained in glycerol under -80oC and were freeze-dried for long-term preservation. A number of the microbial cultures have bioactive potential. The collection is an important addition to the UPCC holdings.
In relation to strengthening the National Quality Infrastructure of the country, Dr. Siringan and Ms. Argayosa have continuously participated as members of various Technical Working Groups (TWGs) involved in the development of microbiological standards for agricultural products. The TWGs were organized by the Bureau of Agriculture and Fisheries Standards (BAFS) of the Department of Agriculture (DA). Their involvement in the said TWG is a continuing commitment to the Bureau’s advocacies and programs on food safety.
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Researches
Implemented in 2017 and continuing in 2019
Project 1 title : Microbial Diversity in the Long-term Fertility Experiment in Irrigated Lowland Rice Ecosystem
Project Leader(s) : Jayvee A. Cruz/Wilfredo Collado Researcher : Maria Auxilia T. Siringan Funding Agency : Department of Agriculture-Regional Field Office Region 3 (DA-RFO3) Project duration : September 1, 2017 to June 30, 2019 (for Year 2 renewal)
Completed in 2018
Program title : Discovery and Development of Health Products: Marine Component (DDHP: Marine) Project title : High-throughput Multi-omics Discovery of Bioactive Marine Compounds and Establishment of a Marine Chemical Library and Informatics Facility
Project Leader : Arturo O. Lluisma (Marine Science Institute/MSI) Study Title : Characterization and Culture of Microbial Isolates Project Staff, Level 3 : Maria Auxilia T. Siringan Science Research Paula Camile D. Inggalla Specialist (SRS) Sharmaine T. Maldia Funding Agency : Department of Science and Technology-Philippine Council for Health Research and Development (DOST-PCHRD) Project Duration` : July 1, 2014 to March 31, 2018
Project 3 title : Anti-infective and Anticancer Drug Candidates: Discovery and Development
Project Leader : Lilibeth Salvador-Reyes (MSI) Study Title : Culture Optimization and Anti-infective Productions by Sponge-derived Marine Microorganisms Project Staff, Level 3 : Maria Auxilia T. Siringan Science Research : Charose Marie T. Perez Specialist (SRS) : J.C.D. Pal Molino Funding Agency : DOST-PCHRD Project Duration : July 1, 2014 to March 31, 2018
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Extension Services/Customers
Profile and Types of Microbiological Services
MRSL has provided microbiological services to the various sectors of the society. The laboratory provided 8,611 microbiological services to different customer types in 2018 (Table 4). This is nearly 20% higher than was recorded in 2017. These comprised of the following: a) 4,915 tests for the bacteriological examination of different types of water (57.08%); b) 3,257 (37.8%) for the microbiological analyses of non-food and non- water samples such as biocidal agents, equipment, extracts from various sources, home care products, personal care products, swab samples and microbial isolates; and c) 439 (5.10%) microbiological analyses of food and beverage. Table 4 and Fig. 10 present the number and percentage of microbiological tests per sample category performed in 2018, respectively.
Table 4. Number of microbiological tests performed for different categories of samples in 2018 Microbiological Analyses Bacteriological of Non-food and Microbiological Examination of Water Non-water Samples Analyses of Total Samples (including identification of Food Samples isolates)
4,915 3,257 439 8,611
Fig. 10. Microbiological services performed by MRSL in 2018 based on sample category.
Of the 4,915 water samples tested, 3,823 (77.78%) were analyzed for coliform counts or potability testing. A total of 1,008 (20.51%) samples were tested for Heterotrophic Plate Count (HPC). Table 5 and Fig. 11 present the different microbiological tests done on various types of water samples in 2018.
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Table 5. Bacteriological services performed on various types of water in 2018. Coliform Count Heterotrophic Pseudomonas Fecal Coliform Total per Customer (Potability Plate Count aeruginosa StreptococcusDe Level Customer Type Testing) (HPC) Detection tection Determination Type
I 0 0 0 0 0 0 II 0 0 0 0 0 0 III 0 0 0 0 0 0 IV 5 28 0 0 10 43 V 247 117 5 5 6 380 VI 3,571 863 22 31 5 4,492 Subtotal 3,823 1,008 27 36 21 TOTAL 4,915 Legend: Customer Types I : College of Science (CS), UP students II : Non-NSRI funded research projects and non-CS, UP students III : UP researchers from other UP units IV : Non-UP students V : Government agencies and Small-to Medium-scale Enterprises VI : Industry
Fig. 11. Bacteriological services performed on various types of water in 2018.
A total of 2,631 microbiological services were performed on non-water samples in 2018 (Table 6 and Fig. 12). This is more than 41% (41.4%) higher than what was recorded in 2017.
Nearly 60% (1,567/2,631 or 59.56%) of the non-water samples submitted for microbiological testing comprised of extracts from various biological samples and matrices. Notably, antimicrobial assay of various extracts and materials was the most requested type of microbiological service provided for the customers, particularly students from various educational institutions (Fig. 12). A number of food samples and personal care products were submitted in relation to microbiological quality assessment and compliance to relevant standards. MRSL continues to provide services that support the goals of its diverse clientele.
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Table 6. List of microbiological services rendered for non-water samples for the period: January to December 2018 Customer Types Code Type of Service I II III IV V VI Total 1 Aerobic plate count 1 0 11 23 60 272 367 2 B. cereus count 0 0 2 0 0 1 3 3 Bifidobacterium count 0 0 0 0 6 0 6 4 BIOLOG ecoplate reading 0 0 0 0 106 0 106 5 Coliform and E. coli count 0 0 0 26 0 102 128 6 Detection of Bacillus 0 0 0 0 0 3 3 7 Detection of C. albicans 0 0 0 0 0 88 88 8 Detection of E. coli 0 2 0 0 60 111 173 9 Detection of Pseudomonas 0 0 2 0 0 115 117 10 Detection of S. aureus 0 0 2 4 60 127 193 11 Detection of Salmonella 0 0 0 1 0 30 31 Detection of selected pathogen in 12 0 0 0 14 0 0 14 swab samples 13 Enterobacteriaceae count 0 0 0 0 0 4 4 14 Growth promotion test 0 0 0 5 0 5 10 Growth of T. mentagrophytes on 15 modified Sabouraud dextrose agar 0 0 0 6 0 0 6 (SDA) 16 Identification of bacterial isolate 16 0 2 9 0 1 28 17 Isolation of fungal isolate 0 0 0 0 0 1 1 18 Lactic acid bacteria count 0 0 0 5 0 55 60 19 Lactobacillus count 0 0 0 0 0 3 3 20 Minimum inhibitory concentration 0 10 0 118 0 11 139
Mold count of T. mentagrophyteson 21 0 0 0 20 0 0 20 modified SDA
22 Percent kill 46 0 3 58 0 61 168 23 Preservative Challenge Test 0 0 0 0 0 25 25
Qualitative screening of 24 142 9 47 1494 0 11 1,703 compounds/antimicrobial assay
25 Yeast and mold count 0 0 11 10 60 219 300 Subtotal 205 21 80 1,793 352 1,245 3,696 TOTAL 3,696 Legend : Customer Types I : College of Science (CS), UP students V : Government agencies and Small-to Medium-scale Enterprises II : Non-NSRI funded research projects and non-CS, UP students Vl : Industry lll : UP researchers from other UP units lV: Non-UP students
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Fig. 12. Microbiological Services Performed on Non-water Samples in 2018.
Profile of Customers
More than 91% (91.39% or 4,492/4,915) of the microbiological tests done for water samples were provided to the industrial sector (Fig.12; Table 6). Only 7.73% of these tests were provided to government agencies and small-to-medium scale enterprises (SMEs), while tests requested by students comprised only 0.87%. Through these services, MRSL has supported the programs of regulatory agencies, water distribution firms and the water industry in ensuring the safety of the water supply.
Fig. 13. Profile of clients who have availed of microbiological tests for water samples in 2018.
For the microbiological services done on non-water samples, the major customers are students from different schools, colleges and universities (Fig. 13; Table 6). More than 48% (1,793/3,696 or 48.51%) of the microbiological analyses
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were provided to non-UP students from elementary, secondary and tertiary educational institutions. Nearly 34% (1,245/3,696) of these services were provided to the industry. Services were also provided to students of the College of Science (CS), UP-Diliman or UPD (5.55%), NSRI-funded researchers (0.57%), UP Diliman researchers and students outside CS (2.16%), and government agencies and small- to-medium scale enterprises (9.52%).
Fig. 14. Profile of clients who have availed of microbiological tests for non-water samples in 2018.
Support to Students
Mentoring of Students
The MRSL implemented on-the-job training (OJT) programs for undergraduate students from state and private educational institutions. Dr. Siringan supervised the training of Mr. Aaron Ima G. Bondoc and Ms. Kimberly Harriz M. Dayrit, both B. S. Biology students from the Pampanga Agricultural State College (PASC) from April 3 to May 4, 2018. The program included lectures on key concepts in microbiology and laboratory exercises on aseptic and culture techniques, test methods for bacteriological examination of water, environmental hygiene monitoring and bacterial characterization using biochemical tests. In July 2018, MRSL hosted Ms. Camille Cruz and Ms. Jamela R. Pangilinan, BS Biology students from Far Eastern University (FEU) who were trained in culture media preparation as part of their practicum. Mr. Reynaldo E. Javate, Jr., MRSL’s Laboratory Technician II, served as trainor.
Dr. Siringan served as thesis reader of two (2) Ms. Microbiolgy students of the Institute of Biology (IB), University of the Philippines, namely, Mr. Michael Carlo C. Mariano and Mr. Jan Eldy L. Daquiaog. Their adviser is Dr. Gil M. Penuliar of the said Institute. She also participated as Thesis Examiner of Ms. Nicole R. Bolo, who successfully defended her M.S. Microbiology Thesis, entitled, “Identification and
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Characterization of Ethanol-producing Acetogens Isolated from Sediment Samples” in May 2018.
Providing Technical Advice and Services to Students
MRSL provided technical advice, expertise and services to students from 60 secondary and 37 tertiary educational institutions. The students were the major clients of the MRSL in 2018. In addition, MRSL Researchers provided gratuitous technical advice on the design, analytical methods and interpretation of test results relevant to their investigatory projects, capstone research and theses.
Linkages
International
World Federation of Culture Collections (WFCC) UPCC is a member of the World Federation of Culture Collections (WFCC). It is registered as WDCM 238. Asian Network of Research Resource Centers (ANRRC) Dr. Siringan is a member of the Executive board of ANRRC from January to December 2018. She will serve as board member for another two year-term, from January 2018 to December 2020. She is also a member of the Subcommittee on International Affairs of the ANRRC.
National
National Codex Alimentarius Organization (Codex) Ms. Vina B. Argayosa is a member of the Sub-Committee on Food Hygiene (SCFH), Ad Hoc Working Group for the Drafting of the National Codex Organization Manual of Operations, convened by the Department of Health (DOH). Philippine Network of Microbial Culture Collection (PNMCC) The University of the Philippines Natural Sciences Research Institute-Culture Collection (UPCC) of the MRSL is an affiliate member of the Philippine Network of Microbial Culture Collection (PNMCC). Philippine Society for Microbiology, Inc. (PSM) Dr. Siringan is a Diplomate of the Philippine Academy of Microbiology (PAM), PSM’s accreditation arm. Dr. Siringan, Ms. Argayosa and Ms. Cereno are PSM life members. Philippine Metrology, Standards, Testing and Quality, Inc. (PhilMSTQ) Dr. Siringan is a member of the Philippine Metrology, Standards, Testing and Quality, Inc. (PhilMSTQ). She serves as lecturer-resource person in PhilMSTQ’s technical briefing for proficiency testing in microbiology in April 2018.
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Trainings conducted in 2018
1) Title of Training : Training Course on Bacteriological Examination of Water
Date : February 19 to 23, 2018 Funding Source : MRSL and Training Fees Lecturers and trainors : Dr. Maria Auxilia T. Siringan, Ms. Vina B. Argayosa, and Ms. Celia A. Cereno Fees : P 14,000/trainee No. of participants : 6 Profile of participants : Lab personnel/analysts from government agencies, testing laboratory, university and water districts
2) Title of Training : Customized Training Course on Microbiological Test Methods for the Sugar-Alcohol Industry
Date : August 13 to 17, 2018 Funding Source : MRSL and Training Fees Lecturers and trainors : Dr. Maria Auxilia T. Siringan, Ms. Vina B. Argayosa, and Ms. Celia A. Cereno Fees : P 20,000/trainee No. of participants : 8 Profile of participants : Lab personnel/analysts of a sugar and alcohol production company
3) Title of Training : Customized Training Course on Microbiological Analysis of Water for a Water Testing Laboratory
Date : September 10 to 14, 2018 Funding Source : MRSL and Training Fees Lecturers and trainors : Dr. Maria Auxilia T. Siringan, Ms. Vina B. Argayosa, and Ms. Celia A. Cereno Fees : P 10,000/trainee No. of participants : 3 Profile of participants : Lab personnel/analysts from food industry and government agencies
4) Title of Training : Training Course on Microbiological Analyses of Foods: Quality Indicators and Gram-negative Pathogens
Date : October 15 to 19, 2018 Funding Source : MRSL and Training Fees Lecturers and trainors : Dr. Maria Auxilia T. Siringan, Ms. Vina B. Argayosa, and Ms. Celia A. Cereno Fees : P 16,000/trainee No. of participants : 7 Profile of participants : Lab personnel/analysts from food industry and government agencies
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MRSL in Action
Fig. 15. a) Mr Reynaldo E. Javate, Laboratory Technician II of MRSL (rightmost) trained Mr. Aaron Ima G. Bondoc (leftmost) and Ms. Kimberly Harriz M. Dayrit (center)of the Pampanga Agricultural State College, in the preparation and sterilization of culture media and materials; b) Mr. Bondoc is shown here performing the swab test as part of the exercise on monitoring environmental hygiene; and c) Ms. Dayrit is shown here conducting the serial dilution-pour plate method to determine viable plate count of microorganisms on surfaces. The OJT was undertaken on April 3 to May 4, 2018.
Fig. 16. MRSL conducted the training course on “Training Course on Bacteriological Examination of Water on February 19 to 23, 2018. Ms. Argayosa (a, rightmost) is shown training the participants in the use of the API identification system to confirm the presence of E. coli, a major indicator of fecal contamination of water. Participants were trained in aseptic techniques and relevant test methods through hands-on exercises in the laboratory (b).
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Fig. 17. MRSL was involved in the capacity building of laboratory personnel involved in the microbiological testing of various commodities. A customized training course on “Microbiological Test Methods for the Sugar-Alcohol Industry” was conducted for a sugar-ethanol production company on August 13 to 17, 2019 (a). MRSL also trained laboratory analysts on relevant principles on food microbiology and safety and selected microbial test methods for food and beverages through the training course on “Microbiological Analyses of Foods: Quality Indicators and Gram-negative Pathogens” held on October 15 to 19, 2018(b).
Fig. 18. Dr. Siringan attended the 10th ANRRC International Meeting in Seoul, South Korea (a; row 1, third from right) on September 5 to 7, 2018. She presented the paper, Probing Microbial Diversity in Rice Rhizosphere-Soil Ecosystem” (b) and attended the ANRRC Board Meeting in which plans and programs of the organization for 2019 were discussed (c; Row 2, rightmost).
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Manpower Development
Activities of MRSL Technical Staff in 2018
1) MARIA AUXILIA T. SIRINGAN (University Extension Specialist IV)
Positions held
Laboratory Supervisor (October 1994 to present), Administrative, Quality and Technical Manager (January 2004 to present, Microbiological Research and Services Laboratory (MRSL), Natural Sciences Research Institute (NSRI), University of the Philippines-Diliman (UPD), Diliman, Quezon City. Member, Natural Sciences Research Institute (NSRI) Academic Personnel Committee (AcPC). January 2017 to July 2019. Chair and Biological Safety Officer, NSRI Safety Committee. 1995 to present. Member, Technical Working Group (TWG) for the Philippine National Standards: Specification and Grading for Chili Peppers, January 2016 to September 2018. Member, Technical Working Group (TWG) for Philippine National Standards (PNS): Principles and Guidelines for National Food Control System. DA-BAFS, Visayas Avenue, Quezon City. January 2017 to present. Member, TWG for the Adoption of Codex Standard on Principles and Guidelines for the Establishment and Application of Microbiological Criteria as Philippine National Standard. January to December 2018. Board Member, Executive Board and Member of the International Affairs Committee, Asian Network of Research Resource Centers (ANRRC). September 2018 to December 2020.
Extension and training activities
Organizer, Lecturer and Trainor. Training Course on “Bacteriological Examination of Water”. MRSL, NSRI, UPD, Diliman, Quezon City. February 19 to 23, 2018. Supervisor and trainor. On-the-job training (OJT) of Mr. Aaron Ima G. Bondoc and Ms. Kimberly Harriz M. Dayrit, B. S. Biology students, Pampanga Agricultural State College. MRSL, NSRI, UPD, Diliman, Quezon City. April 3 to May 4, 2018. Resource Person. Lecture on “Proficiency Testing”. 2018 National Microbiological Proficiency Testing Programme Technical Briefing, NSRI Conference Room, UPD, Diliman, Quezon City. April 20, 2018. Resource Person. Bio-Rad Laboratories and Lifeline Diagnostics Supplies, Inc.-organized Seminar on “The Advancement of Food Safety Diagnostics in Microbiological Perspective”, Marco Polo Hotel, Ortigas, Pasig City. May 31, 2018. Supervisor, OJT of Ms. Camille Cruz and Ms. Jamela R. Pangilinan, BS Biology students, Far Eastern University (FEU), MRSL, NSRI, UPD, Diliman, Quezon City. July 2018. Organizer, Lecturer and Trainor. Customized Training Course on “Microbiological Test Methods for the Sugar-Alcohol Industry”, MRSL, NSRI, UPD, Diliman, Quezon City. August 13 to 17, 2018. Lecturer and trainor. Customized Training Course on “Microbiological Analysis of Water for a Water Testing Laboratory”, MRSL, NSRI, UPD, Diliman, Quezon City. September 10 to 14, 2018. Organizer, Lecturer and Trainor. Training Course on “Microbiological Analyses of Food: Quality Indicators and Gram-Negative Pathogens”, MRSL, NSRI, UPD, Diliman, Quezon City. October 15 to 19, 2018. Supervisor, Coordinator and Trainor. On-the-job Training of Ms. Mary Ann Cielo R. San Diego. University Researcher I. MRSL, NSRI, UPD, Diliman, Quezon City. September 15 to December 20, 2018.
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Oral and poster paper presentations
Siringan, M. A. T., P. C. D. C. Ingalla, S. T. Maldia, J. C. P. D. Molino, C.M. T. Perez,G. A. V. Torres and L. Salvador-Reyes. Oral paper on “Searching for Bioactive Sponge-associated Marine Microorganisms Through Optimized Protocols”. Presented during the 47th PSM Annual Convention and Scientific Meeting, Oriental Legazpi Hotel, Legazpi City, Albay. July 19-20, 2018. Siringan, M. A. T., P. J. M. Pangilinan, W. B. Collado, E. V. Evangelista and J. A. Cruz. Oral paper on “Probing Microbial Diversity in Rice Rhizosphere-Soil Ecosystem”, Presented during the 10th ANRRC International Meeting. in Korea Science and Technology Center (KSTC), Seoul, South Korea. September 18, 2018.
Publication
P. De Leon M., A. D. Montecillo, D. S. Pinili, M. A. T. Siringan and D. S. Park. 2018. Bacterial diversity of bat guano from Cabalyorisa Cave, Mabini, Pangasinan, Philippines: A first report on the metagenome of Philippine bat guano. PLOS ONE 13(7): e0200095. https://doi.org/10.1371/journal.pone. 0200095. (Date of publication: July 19, 2018. Obusan, M C. M., L. V. Aragones, W. L. Rivera, and M. A. T. Siringan. 2018. Antibiotic susceptibility patterns of bacteria isolated from cetaceans stranded in the Philippines. Aquatic Mammals. 44(5):558.569, doi 10.1578/AM.44:5.2018.558. (Date of Publication: September 2018).
Conferences/Seminars/Symposia/Trainings attended
NSRI-PAM Seminar on “The Intriguing SPIRALS: Link to Food Safety and Gut Health”, Presented by Dr. Manuel M. Garcia. College of Science, UPD, Diliman, Quezon City. January 29, 12018. 3M Food Safety Seminar on “Overview of the ISO 16140 (including ISO 16140-3) 2018, Microbial Method Harmonization – Global Schemes and Harmonization and 3M Food Safey Rapid Solutions”, 3M Customer Technical Center, Cocolight Building, 11th Ave. cor. 39th St., BGC, Taguig City. March 7, 2018. Seminar on “Microbial Diversity in the long-Term Fertility Experiment in Irrigated Lowland Rice Ecosystem Planning Workshop”, Philippine Rice Research Institute (PhilRice), Muñoz, Nueva Ecija. March 16, 2018. PSM Cluster 2 Symposium, “Gearing up Towards a Globally Competitive, Accredited and Certified Philippine Microbiology”, BGPOP building, University of Santo Tomas, Manila. March 24, 2018. Seminar on “Anti-Sexual Harassment, Orientation to the UP Anti-Sexual Harassment Code 2017 and Gender Orientation and Sensitivity”, NSRI Conference Room, NSRI, UPD, Diliman, Quezon City. June 13, 2018. Seminar on Establishment and Management of BRCs/Biobanks: A Case of KNRRC”, presented by Dr. Ahn Kyungsook, National Institute of Molecular Biology and Biotechnology (NIMBB), UPD, Diliman, Quezon City, July 17, 2018. 47th PSM Annual Convention and Scientific Meeting, Oriental Legazpi Hotel, Legazpi City, Albay. July 19-20, 2018. 10th ANRRC International Meeting, Korea Science and Technology Center (KSTC), Seoul, South Korea. September 18, 2018. World Experts Lecture Series, “Protozoan Interactions Influence Abundance of Genes Encoding Heavy Metal Resistances in the Environment”, presented by Dr. Christopher G. Rensing, IB, UPD, Diliman, Quezon City. November 12, 2018. Industry Stakeholders Forum on the Adoption of ASEAN Standard for Spinach, Tomatoes, Calamansi and Limes, and Taro Leaves and Stem. November 27, 2018.
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Mentoring Activities
Thesis Reader of Mr. Michael Carlo C. Mariano, MS Microbiology candidate, Institute of Biology (IB), UP-Diliman (UPD), for the thesis entitled, “Multilocus Sequence Typing and Virulence Genes Profiling of Campylobacter jejuni Isolated from Chicken Products in Metro Manila. March 2018 to present. Thesis Reader of Mr. Jan Eldy L. Daquiaog, MS Microbiology candidate, IB, UPD, for the thesis entitled, “Isolation and Characterization of Actinomycetes with Enzymatic and Antibacterial Activities from Soils Collected in University of the Philippines Diliman”. March 2018 to present. Thesis Examiner of Ms. Nicole R. Bolo, MS Microbiology, IB, UPD, for the thesis entitled, “Identification and Characterization of Ethanol-producing Acetogens Isolated from Sediment Samples”. Successfully defended in May 2018. Co-adviser of Mr. Luis Angelo Cortez, M. S. Environmental Science candidate, Institute of Environmental Science and Meteorology (IESM), UPD, for the thesis entitled, “Fecal Enterococci Loading in Selected Tributaries of the Pampanga River Basin and its Relation to Land Use. July 2018 to present. External Thesis Examiner of Ms. Johanna A. Bangoy, Ms. Microbiology candidate, UP_Los Banos Graduate School (UPLBGS), for the thesis entitled, “Microbiological, Physico- chemical and Organoleptic Assessment of a Carrot-Milk Drink Formulation using Lactococcus lactis [(Lister) Schleifer et al.] SC1, Lactobacillus plantarum [(Orla-Jensen) Bergey et al.] BS and Pediococcus acidilactici (Lindner) 3G3 as Potential Probiotic Starter Cultures”. May 30, 2018.
2) VINA B. ARGAYOSA (University Researcher II)
Positions Held
Deputy Technical Manager. MRSL, NSRI, UPD, Diliman, Quezon City. January 2004 to present. Member, TWG for Philippine National Standards (PNS): Principles and Guidelines for the Conduct of Microbiological Risk Assessment and Working Principles for Risk Analysis for Food Safety Application by Governments. DA-BAFS, Visayas Avenue, Quezon City. 2016 to present. Member, TWG for Philippine National Standards (PNS): Principles and Guidelines for National Food Control System. DA-BAFS, Visayas Avenue, Quezon City. January 2017 to present. Member, TWG for the Adoption of Codex Standard on Principles and Guidelines for the Establishment and Application of Microbiological Criteria as Philippine National Standard. January to December 2018.
Extension and training activities
Lecturer and Trainor. Training Course on “Bacteriological Examination of Water”, MRSL, NSRI, UPD, Diliman, Quezon City. February 19 to 23, 2018. Lecturer/Resource Person. Lecture on “Basic Microbiology and Activities of the MRSL” for EnE303 Biological Processes in Environmental Engineering, MRSL, NSRI, UPD, Diliman, Quezon City. March 5, 2018. Trainor. On-the-job training of Ms. Jamelle Justine C. Cataag, University Research Associate I (URA I), MRSL, NSRI, UPD, Diliman, Quezon City. February 1 to June 30, 2018. Trainor. On-the-job training (OJT) of Mr. Aaron Ima G. Bondoc and Ms. Kimberly Harriz M. Dayrit, B. S. Biology students, Pampanga Agricultural State College, MRSL, NSRI, UPD, Diliman, Quezon City. May 3 to 4, 2018. Lecturer and Trainor. Customized Training Course on “Microbiological Test Methods for the Sugar-Alcohol Industry”, MRSL, NSRI, UPD, Diliman, Quezon City. August 13 to 17, 2018.
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Lecturer and trainor. Customized Training Course on “Microbiological Examination of Water” for a Water Testing Laboratory, MRSL, NSRI, UPD, Diliman, Quezon City. September 10 to 14, 2018. Lecturer/Resource Person. Lecture on “Applied Microbiology in the Laboratory” for EDH 110 Applied Microbiology and Epidemiology in Health Education. MRSL, NSRI, UPD. Diliman, Quezon City. September 21, 2018. Lecturer and Trainor. Training Course on “Microbiological Analyses of Food: Quality Indicators and Gram-Negative Pathogens”. MRSL, NSRI, UPD, Diliman, Quezon City. October 15 to 19, 2018. Trainor. On-the-job Training of Ms. Mary Ann Cielo R. San Diego. University Researcher I. MRSL, NSRI, UPD, Diliman, Quezon City. September 15 to December 20, 2018.
Conferences/Seminars/Symposia/Trainings attended
NSRI-PAM Seminar on “The Intriguing SPIRALS: Link to Food Safety and Gut Health”, Presented by Dr. Manuel M. Garcia. College of Science, UPD, Diliman, Quezon City. January 29, 12018. 3M Food Safety Seminar on “Overview of the ISO 16140 (including ISO 16140-3) 2018, Microbial Method Harmonization – Global Schemes and Harmonization and 3M Food Safey Rapid Solutions”, 3M Customer Technical Center, Cocolight Building, 11th Ave. cor. 39th St., BGC, Taguig City. March 7, 2018. PSM Cluster 2 Symposium, “Gearing up Towards a Globally Competitive, Accredited and Certified Philippine Microbiology”, BGPOP building, University of Santo Tomas, Manila. March 24, 2018. Seminar on “Anti-Sexual Harassment, Orientation to the UP Anti-Sexual Harassment Code 2017 and Gender Orientation and Sensitivity”, NSRI Conference Room, NSRI, UPD, Diliman, Quezon City. June 13, 2018. Seminar on Establishment and Management of BRCs/Biobanks: A Case of KNRRC”, presented by Dr. Ahn Kyungsook, National Institute of Molecular Biology and Biotechnology (NIMBB), UPD, Diliman, Quezon City. July 17, 2018. Training course on “Water Microbiological Analysis”. National Reference Laboratory (NRL), Department of Health (DOH), East Avenue Medical Center (EAMC), East Avenue, Quezon City. August 7 to 10, 2018.
3) CELIA A. CERENO (University Researcher II)
Positions Held
Deputy Administrative Manager and Financial Manager. MRSL, NSRI,UPD, Diliman, Quezon City. January 2004 to present.
Extension and Training activities
Lecturer and Trainor. Training Course on “Bacteriological Examination of Water”, MRSL, NSRI, UPD, Diliman, Quezon City. February 19 to 23, 2018. Lecturer and Trainor. Customized Training Course on “Microbiological Test Methods for the Sugar-Alcohol Industry”, MRSL, NSRI, UPD, Diliman, Quezon City. August 13 to 17, 2018. Organizer, lecturer and trainor. Customized Training Course on “Microbiological Analysis of Water” for a Water Testing Laboratory, MRSL, NSRI, UPD, Diliman, Quezon City. September 10 to 14, 2018. Lecturer and Trainor. Training Course on “Microbiological Analyses of Food: Quality Indicators and Gram-Negative Pathogens”. MRSL, NSRI, UPD, Diliman, Quezon City. October 15 to 19, 2018.
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Trainor. On-the-job Training of Ms. Mary Ann Cielo R. San Diego. University Researcher I. MRSL, NSRI, UPD, Diliman, Quezon City. September 15 to December 20, 2018.
Conferences/Seminars/Symposia/Trainings attended
NSRI-PAM Seminar on “The Intriguing SPIRALS: Link to Food Safety and Gut Health”, Presented by Dr. Manuel M. Garcia. College of Science, UPD, Diliman, Quezon City. January 29, 12018. Seminar on “Microbial Diversity in the long-Term Fertility Experiment in Irrigated Lowland Rice Ecosystem Planning Workshop”, PhilRice, Muñoz, Nueva Ecija. March 16, 2018. Seminar on “Anti-Sexual Harassment, Orientation to the UP Anti-Sexual Harassment Code 2017 and Gender Orientation and Sensitivity”, NSRI Conference Room, UPD, Diliman, Quezon City. June 13, 2018.
4) MARY ANN CIELO R. SAN DIEGO (University Researcher I)
Conferences/Seminars/Symposia/Trainings attended
On-the-job training (OJT) in MRSL Quality Management System (QMS), relevant Management and Technical Standard Operating Procedures (Microbial test methods for the examination of water and microbiological analyses of food). MRSL, NSRI, UPD, Diliman, Quezon City. September to December 2018. OJT in Culture Collection Techniques and Fungal Culture Preservation. MRSL, NSRI, UPD, Diliman, Quezon City. September to December 2018. Customized Training Course on “Microbiological Analysis of Water” for a Water Testing Laboratory, MRSL, NSRI, UPD, Diliman, Quezon City. September 10 to 14, 2018. Scientific symposium on “Forging research collaborations, moving forward together”. IB, UPD. September 11, 2018. NSRI Laboratory Safety Seminar on “Safety and Security in the Workplace: A Primer on NSRI’s Safety and Security Rules and Guidelines”, NSRI Conference Room, NSRI, UPD, Diliman, Quezon City. September 17, 2018. Training Course on “Microbiological Analyses of Food: Quality Indicators and Gram- Negative Pathogens”. MRSL, NSRI, UPD, Diliman, Quezon City. October 15 to 19, 2018.
Publication
Justo, C. A. C., M. A. C. V. Relucio-San Diego and W. L. Rivera. 2018. Metronidazole susceptibility and TVV-infection of Trichomonas vaginalis from Metro Manila and Angeles City, Philippines”, Acta Medica Philippina. 52:6 (Date of Publication: December 31, 2018)
Activities of the MRSL Support Staff in 2018: Seminars attended
1) REYNALDO E. JAVATE, JR. (Laboratory Technician II)
Seminar on “Microbial Diversity in the long-Term Fertility Experiment in Irrigated Lowland Rice Ecosystem Planning Workshop”, PhilRice, Muñoz, Nueva Ecija. March 16, 2018. Seminar on “Anti-Sexual Harassment, Orientation to the UP Anti-Sexual Harassment Code 2017 and Gender Orientation and Sensitivity”, NSRI Conference Room, UP-Diliman, QC. June 13, 2018.
2) ROSALYN F. DIAZ (Administrative Aide V)
Seminar on “Microbial Diversity in the long-Term Fertility Experiment in Irrigated Lowland Rice Ecosystem Planning Workshop”, PhilRice, Muñoz, Nueva Ecija. March 16, 2018.
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Seminar on “The Overview on Procurement Modes and Introduction of New Process and Template for Small Value Procurement”, NSRI, UPD. April 18, 2018. Seminar on “Anti-Sexual Harassment, Orientation to the UP Anti-Sexual Harassment Code 2017 and Gender Orientation and Sensitivity”, NSRI Conference Room, UP-Diliman, QC. June 13, 2018.
3) MARY ADELE ARJONA (Administrative Aide II)
Seminar on “Microbial Diversity in the long-Term Fertility Experiment in Irrigated Lowland Rice Ecosystem Planning Workshop”, PhilRice, Muñoz, Nueva Ecija. March 16, 2018.
4) DIONESIO R. VILLANUEVA (Administrative Aide I)
Seminar on “Microbial Diversity in the long-Term Fertility Experiment in Irrigated Lowland Rice Ecosystem Planning Workshop”, PhilRice, Muñoz, Nueva Ecija, March 16, 2018. “Pagsasanay sa Gawaing Adbokasiya at Pag-oorganisa”, College of Social Work and Community Development. UPD, Quezon City. April 20 to 21, 2018. Seminar on “Anti-Sexual Harassment, Orientation to the UP Anti-Sexual Harassment Code 2017 and Gender Orientation and Sensitivity”, NSRI Conference Room, NSRI, UPD, Diliman, Quezon City. June 13, 2018.
5) RAQUEL O. TIMOLA (Utility Worker, non-UP Contractual)
Seminar on “Microbial Diversity in the long-Term Fertility Experiment in Irrigated Lowland Rice Ecosystem Planning Workshop”, PhilRice, Muñoz, Nueva Ecija, March 16, 2018 Seminar on “Anti-Sexual Harassment, Orientation to the UP Anti-Sexual Harassment Code 2017 and Gender Orientation and Sensitivity”, NSRI Conference Room, NSRI, UPD, Diliman, Quezon City. June 13, 2018.
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RESEARCH AND ANALYTICAL SERVICES
LABORATORY (RASL)
The Research and Analytical Services Laboratory (RASL) is the only chemical laboratory among the four research and technical services laboratories of the NSRI. It performs two core functions of the University, i.e., as a research university and as a public service university. Though limited in scope, the laboratory also participates in the function of the university as a graduate university through mentorship of graduate students utilizing the available expertise of its staff and the facilities of the laboratory.
Fig. 19. The REPS and Administrative Staff of the RASL.
Researches
As a research laboratory, the RASL focuses its research in the areas of analytical and environmental chemistry, specifically on the development and/or validation of highly sensitive analytical methods for identification and measurement of various chemical components in environmental and food samples, as well as on the fate and transport of emerging chemical pollutants in the environment. In the past 18 years, it has established its capabilities to conduct researches on endocrine disrupting compounds and persistent organic pollutants such as phenolic substances, pesticides, polybrominated diphenyl ethers (PBDEs), polychlorinated biphenyls (PCBs) in various compartments of the Philippine environment (water, sediments and biota).
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Externally-funded
Foreign
1) Project title : Perfluorinated Compounds in Water, Sediments, Biota and Drinking Water in the Philippine Environment
Project leader : Dr. Charita S. Kwan (UNU-IAS designated National Project Coordinator for the Philippines Project staff : Ms. Kristine O. Manalang (additional assignment) Ms .Jannelle O. Cristobal (additional assignment Mr. Vergel G. Valenzuela (additional assignment) Funding source : United Nations University-Institute for the Advanced Study of Sustainability in cooperation with Shimadzu Corporation Duration of the project : May 2017 – May 2018 (Phase VII -Year II) ` August - December 2018 (Phase VII- Year III)
2) Project title : Global Atmospheric Sampling (PUF-Disk Sampling): Activities in the Philippines
Project leader : Dr. Charita S. Kwan (Implementor – Philippines) Project staff : Ms. Kristine O. Manalang (additional assignment) Mr. Vergel G. Valenzuela (additional assignment) Funding source : Environment and Climate Change Canada, Government of Canada – Air Quality Research Division, Science and Technology Branch Duration of project : January 1 – December 31, 2018
Local
1) Project title : Developing Information Infrastructure for Managing Antibiotics and Endocrine Disrupting Substances in Pampanga River Basin and its Coastal Environs: Maps, Transport Models and Bioindicators of Ecological and Public Health Risks
Project staff : Dr. Charita S. Kwan (Level III and Collaborator) (Environmental Chemistry) : Ms. Aira Sacha Nadine S. Ferrer (Research Fellow II) Funding source : Commission on Higher Education – Philippine California Advance Research Institute (CHED-PCARI) Duration of project : June 16, 2019 (start of the two-year project)
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Fig. 20. Mr. Adriel John R. Ilagan and Mr. Patrick Pestilos (partly hidden) taking water samples from the Pasig River for the monitoring of perfluorinated compounds for the UNU-IAS funded project on Monitoring and Management of POPs in Asia.
Fig. 21. Ms. Aira Sacha Nadine S. Ferrer during the first sampling trip in Pampanga River (top picture) and with the PhD and MS Environmental Engineering students (bottom) for the CHED-PCARI project antibiotics and endocrine disrupting substances in the Pampanga River Basin.
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2) Project title : Chemical Safety and Waste Management of NSRI
Researcher : Dr. Windell L. Rivera Project staff : Kristine O. Manalang (Pollution Control Officer) Vergel. G. Valenzuela (Pollution Control Officer)) Adriel John R. Ilagan (Chemical Waste Management Associate Funding source : NSRI Duration of project : January to December 2018
3) Project title : Strengthening Knowledge of Chemical Safety and Waste Management in Academic Laboratories: A Service-Learning Project of the UP NSRI Research and Analytical Services Laboratory for Public Secondary Schools
Project staff : Ms. Jonah L. Bondoc (Project Leader) Dr. Charita S. Kwan (Team Member Ms. Kristine O. Manalang (Team Member) Mr. Vergel G. Valenzuela (Team Member) Funding source : OVCRD Duration of project : December 1, 2018 (start of one-year project)
In-house
Members of the research staff of the RASL engage in two types of short-term in-house research projects:
Expanding the capabilities of the RASL to provide chemical analyses to the general public
- methods development and validation of chemical methods of analysis - review and revision of technical and management procedures for the compliance of the requirements of accreditation bodies, specifically to the latest ISO 17025:2017 standard.
Development projects are mostly funded by laboratory-generated funds.
Research Program : (a) Towards Re-accreditation of the RASL to ISO 17025:2017 : (b) Preparation of documents according to ISO 17025:2017 standard
Duration : (a) January to June 2018 (b) July to December 2018
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Researchers Title of Sub-Projects
Dr. Charita S. Kwan Revision of the Quality Manual, QA Protocols and Procedure on Evaluation of Laboratory Performance
Procedures on Selection, Verification and Validation of Method, Management System Documentation and Management Review
Ms. Jonah L. Bondoc Revision of Procedure on Quality Protocols Pertaining to Service to Customers
Procedures on Personnel, Review of Requests, Tenders and Contracts, Complaints, Actions to Address Risks and Opportunities, and Improvement
Ms. Kristine O. Manalang Revision of Analytical Procedures and Validation Data for the Assigned Analyses According to the RASL-ISO 17025 Format
Revision of the Procedures on Management of Laboratory Environment and on Documentation and Control of Laboratory Procedures
Procedures on Metrological Traceability, Externally Provided Products and Services, Sampling, Ensuring the Validity of Results, Reporting of Results, Non- conforming Work, Corrective Actions, and Internal Audits
Mr. Vergel G. Valenzuela Revision of Analytical Procedures and Validation Data for the Assigned Analyses According to the RASL-ISO 17025 Format
Revision of the Technical Procedures for Calibration of Laboratory Equipment and the Facilities and Environmental Conditions
Documented Procedure for the Calculation of Measurement Uncertainty in Chemical Analysis
Ms. Abegail R. Responde Revision of Analytical Procedures and Validation Data for the Assigned Analyses According to the RASL-ISO 17025 Format.
Collation of Revised Analytical Procedures for Inorganic and Organic Analyses
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Procedures on Technical Records, Control of Data and Information Management, Control of Management System Documents, Control of Records
Collation of All Documents Required by the ISO 17025:2017 Standard
Research towards obtaining graduate degrees (Chemistry or in other allied disciplines)
REPS/Degree Title of Research
Ms. Jonah L. Bondoc PCB Levels in Lipid-rich Tissues of Stranded PhD Environmental Science Cetaceans Found in the Philippines in Correlation to candidate their Toxic Equivalents and Histopathological Results
Ms. Kristine O. Manalang Quantitative Determination of OP Pesticides in MS Chemistry Surface Water of Agricultural Fields
Ms. Jannelle O. Cristobal Thermostability of Chloramphenicol in Fish using MS Food Science Simulated Thermal Food Processing Treatments Commonly Used in Food Preservation.
RASL Papers Presented
• Kwan, C.S., Manalang, K.O., Cristobal, J.O., Valenzuela, V.G. "A Glimpse of the Occurrence of PFCs in the Philippine Environment", 27th Symposium on Environmental Chemistry. Okinawa Prefecture City, Town and Village Council Hall, Okinawa, Japan. May 24, 2018. • Bondoc, JL, Kwan, CS, Aragones, LV. “Banned PCBs – How Much is Left Out There? Results from Stranded Dolphins and Whale in the Philippines”, 33rd Philippine Chemistry Congress, Philippine International Convention Center, Manila. May 30 to June 1, 2018. • Bondoc, JL Kwan, CS, Aragones, LV. “Assuring the Quality of Analytical Data: PCBs in High-Fat Sample Matrices”, 33rd Philippine Chemistry Congress, Philippine International Convention Center, Manila. May 30 to June 1, 2018. • Valenzuela, V.G. and Kwan, C.S. “Assuring the Quality of Analytical Data: TOC in Pharmaceutical Waters”. 33rd Philippine Chemistry Congress. City of Manila. Organized by the Integrated Chemists of the Philippines. May 30 to June 1, 2018.
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Fig. 22. Dr. Charita S. Kwan at the 27th Symposium on Environmental Chemistry in Okinawa, Japan on May 24, 2018.
Fig. 23. Ms. Kristine O. Manalang (Left) and Mr. Vergel G. Valenzuela (Right) during the poster presentation for the 27th Symposium on Environmental Chemistry, Okinawa, Japan, May 22 to 25, 2018.
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Fig. 24. Dr. Charita S. Kwan, Ms. Kristine O. Manalang and Mr. Vergel G. Valenzuela during the 27th Symposium on Environmental Chemistry and the UNU- IAS Project Meeting in Okinawa, Japan, May 22 to 24, 2018
Fig. 25. Ms. Jonah L. Bondoc during paper presentation for the 33rd Philippine Chemistry Congress, Philippine International Convention Center, Manila, May 30 to June 1, 2018.
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Fig. 26. Mr. Vergel G. Valenzuela during paper presentation for the 33rd Philippine Chemistry Congress, Philippine International Convention Center, Manila, May 30 to June 1, 2018.
Extension Services
As a public service laboratory, the RASL conducts extension services by providing chemical testing services and training programs to serve the needs of students, other researchers, staff of government institutions, the private sector and the general public.
I. Chemical Testing Services
The RASL is a provider of chemical testing services:
Chemical parameters in drinking water and in bottled water as specified by the Department of Health and by the Food and Drug Administration (FDA) Philippines, respectively.
Total Organic Carbon (TOC) in pharmaceutical waters as specified by FDA Philippines.
Chemical tests that are not commonly done by other chemical laboratories Polycyclic aromatic hydrocarbons (PAHs) in consumer products such as smoked fish, coconut oil and beer products.
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RASL Credentials
Compliant to RA 10657 (Chemistry Profession Act)
Awarded the Certificate of Authority to Operate a Chemical Laboratory No. 009 by the Professional Regulation Commission (PRC) Board of Chemistry on January 30, 2018.
Fig. 27. The Certificate of Authority to Operate No. 009 of the RASL.
Fig. 28. Dr. Charita S. Kwan (2nd from right) and Ms. Jonah L. Bondoc (rightmost) with the PRC Board of Chemistry officials during the awarding of the Certificate of Authority to Operate to the RASL during the 33rd Philippine Chemistry Congress at the Philippine International Convention Center, Manila, May 30 to June 1, 2018.
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Committed to deliver quality test results – Laboratory of Excellence in the evaluation of Total Organic Carbon in water for two consecutive years.
Fig. 29. The Laboratory Excellence given to the RASL by the Environmental Resource Associates, U.S.A., a global provider of proficiency testing products and certified reference materials, in recognition of the superior quality of the RASL in the evaluation of Total Organic Carbon (TOC) in water based on its participation in the Proficiency Testing Round 268 in November 2018.
On its re-accreditation to ISO/IEC 17025:2017 as a testing laboratory, the RASL, is challenged by the new requirement of the standard, i.e., all support services of the laboratory such as procurement and human resources must be ISO certified, e.g., to the ISO 9001:2015.
Despite this constraint, the RASL continuously works toward revisions of pertinent laboratory documents in order to meet the requirements of the new ISO 17025:2017 standard. It also continuously strives to provide reliable analytical data through adherence to the QA/QC protocols of the laboratory.
A. Analytical Instruments Available
Atomic Absorption Spectrophotometer Electrochemical Analysis Assembly Gas Chromatograph/Mass Spectrometer Graphite Furnace Atomic Absorption Spectrophotometer Ion Chromatograph Liquid Chromatograph/Mass Spectrometer/Mass Spectrometer Microwave Digestor Total Organic Carbon Analyzer
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B. Customers Served in 2018
Type I UP College of Science Students Type II UP NSRI-Funded Researches Type III UP Diliman Students outside of the College of Science Type IV UP-Funded Researches outside NSRI and Students of UP outside Diliman Type V Government, Small and Medium Scale Industries, and Private Institutions Type VI Multinational Companies (Top 1000) PT Participation in Proficiency Testing
Fig. 30 . Customers Served by the RASL in 2018
Support to Researchers and Students
Chemical Testing Services
Table 7. List of students and researchers provided with chemical testing services for their research, dissertation/thesis or class projects in 2018. Degree / Type of School / University Name Title Course Assistance
National Institute of Mary Ann Cielo R. Research Analysis of feed (tap) Analysis of Molecular Biology San Diego water for UP NIMBB Calcium, and and Biotechnology distiller Magnesium in (NIMBB) University and Hardness of the Philippines of Water Diliman Sample Institute of Biology, Dr. Michael C. MS Gene Expression Analysis of University of the Velarde / Windy Microbiology Analysis Copper- Copper in Philippines Diliman Lou R. Bagay Tolerant Endophytic Prepared Fungi Isolated from a Solutions, Plant Found in Mine Plant and Soil Tailings / Transcriptome Samples Analysis of Copper- Tolerant Endophytic Fungi Isolated from Mine Tailings
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Cont. of Table 7. University of the Marie Fatima S. High School Research Project Analysis of Philippines Paraiso Copper, Iron Integrated School and Total Dissolved Solids in Water Samples Department of Mary Vermi Aizza MS Research Project Analysis of Environmental M. Corpuz Environmental Lead in Water Engineering, Engineering Samples College of Engineering, University of the Philippines Diliman
Technical Advisories / Consultation Services
Technical advisories or consultation services on chemical analyses and other related topics are provided free to students and to the general public, whenever requested.
Type of Students Number of Consultations Feedback*
High School 28 100% Best
College 5 80% Best; 20% Better
*Based from students who voluntarily accomplished the survey forms, i.e., only 28.5% and 100% of the entire consultation services provided to high school and college students, respectively.
II. Training Programs
Provide training programs that are aimed towards capacity building of laboratory personnel of other chemical laboratories in the country through the technical expertise of the RASL Staff and/or the limited resources of the RASL.
Compliant to Republic Act No. 10912 (Continuing Professional Development Act of 2016) and as PRC accredited CPD Provider in Chemistry by the CPD Council of Chemistry (Accreditation No. CHM-2017-006) the RASL resumed the offerings of training programs.
All training programs had approved CPD units (ranging from 6 (one-day course) to 18 (three-day course) to the participants.
Total of five PRC-accredited training programs were offered.
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1) Title of Training : Quality Assurance in Chemical Analysis
Description : A three-day lecture-workshop covering the topic on quality protocols in chemical analysis including basic statistics. The technical aspects of QA as described by CITAC/Eurachem Guide such as selection of method, method validation, measurement of uncertainty, metrological traceability including quality control both internal and external procedures were emphasized in ensuring the quality of analytical data generated by a laboratory. Participants were taught on how to make and use a quality control chart to maintain the quality standard of the analysis. Exercises focused on gravimetric, titrimetric, chromatographic and spectropho-tometric methods of analysis.
Date : April 4-6, 2018 (1st Batch) July 18-20, 2018 (2nd Batch) Funding source : Training fee of P 10,000.00/participant (including food and training manual) Lecturers and trainors : Dr. Charita S. Kwan, Jonah L. Bondoc, Kristine O. Manalang, Vergel G. Valenzuela and Abegail R. Responde Profile of participants : Chemists and other chemistry-related Professionals from government and private institutions
Fig. 31. RASL staff with the participants of the Training Course on “Quality Assurance in Chemical Analysis” conducted on April 4-6, 2018 (1st Batch).
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2) Title of Training : Validation of Chemical Methods of Analysis
Description : A three-day lecture-workshop which covered the topic on validation of chemical methods of analysis including basic statistics. Lectures on how to validate test methods and how to establish the performance characteristics of the method were presented. Participants were taught on how to determine linearity of the calibration curve, range, detection limits, accuracy, accuracy, precision and ruggedness of the analytical method. Exercises focused on gravimetric, titrimetric, spectrophotometric and chromatographic methods of analysis.
Date : August 29-31, 2018 Funding source : Training fee of P 10,000.00/participant (including food and training manual) Trainors : Dr. Charita S. Kwan, Jonah L. Bondoc, Kristine O. Manalang, Vergel G. Valenzuela and Abegail R. Responde Profile of participants : Chemists and other chemistry-related Professional from government and private institutions
Fig. 32. RASL staff with the participants of the Training Course on “Validation of Chemical Methods of Analysis” conducted on August 29-31, 2018.
3) Title of Training : Validation of Chemical Methods of Analysis for the Staff of the Philippine Associated Smelting and Refining (PASAR) Corporation On-site training at the Leyte Industrial Development Estate, Isabel, Leyte
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Description : The training program was tailored-fit to the requirements of PASAR Corp., i.e., validation of the chemical methods of gravimetry, titrimetry, inductively-coupled plasma – optical emission spectrometry (ICP-OES) and X-ray fluorescence (XRF). Establishment of the performance characteristics of the methods based on the guidelines and principles of the Eurachem Guide 2014, ISO/IEC 17025:2017, International Union of Pure and Applied Chemistry (IUPAC) and the Association of Analytical Chemists (AOAC) were taught to the participants.
Date : October 23-25, 2018 Funding source : Agreed training package including air fares, food and accommodation of the trainors. Trainors : Dr. Charita S. Kwan, Dr. Preciosa Corazon B. Pabroa of the Philippine Nuclear Research Institute and Vergel G. Valenzuela Profile of participants : Laboratory personnel of PASAR Corp.
Fig. 33. Top: Dr. Charita S. Kwan (5th from the right, standing), Dr. Preciosa Corazon B. Pabroa (7th from the right, standing center) and Vergel G. Valenzuela (2nd from the right, sitting) with the training participants from PASAR Corp.; Bottom; Dr. Preciosa Corazon B. Pabroa and Mr. Vergel G. Valenzuela (4th
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and 5th from the right, respectively) checking the workshop outputs of the participants.
4) Title of Training : Seminar/Workshop on Chemical Safety and Chemical Waste Management
Description : A two-day lecture-workshop Chemical Waste Management covering the topic on chemical safety and chemical waste management in chemical laboratories. Safety practices on handling of chemicals and laboratory operations including the use of personal protective equipment (PPE) and safety gadgets have been emphasized. Participants were taught about Philippine regulations on chemicals and other pertinent environmental laws. Practices and procedures for an effective chemical waste management program were also presented.
Date : December 6 – 7, 2018 Funding source : Training fee of P 7,500.00/participant Trainors : Jonah L. Bondoc, Kristine O. Manalang and Vergel G. Valenzuela Profile of participants : Chemists and other chemistry-related Professionals from government and private institutions
Fig. 34. RASL trainors and staff with the participants of the Training Course on “Seminar/Workshop on “Chemical Safety and Chemical Waste Management” conducted on December 6 -7, 2018.
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The RASL continues to support selected incoming senior college students pursuing B.S. degree in chemistry or other related fields through On-the-Job Trainings and/or Internship Programs.
The Mid-year 2018 Chemistry 197 (Special Topics in Applied Chemistry – Practicum) - Internship Program on Chemical Analysis and Instrumentation in July 2018 (200 hours).
Training Coordinator : Dr. Charita S. Kwan (Training Coordinator) Trainor(s) : Ms. Jonah L. Bondoc (Lead) Ms. Kristine O. Manalang Ms. Abegail R. Responde Mr. Vergel G. Valenzuela Student Trainees/University : Russel Dane T. Terrado and Samantha Angela B. Valdez / Institute of Chemistry, UP Diliman, Quezon City, Quezon City (200 hours)
Linkages
International
• United Nations University - Institute for the Advanced Study of Sustainability Tokyo, Japan • Tokyo University of Agriculture and Technology – Laboratory of Organic Geochemistry • Shimadzu Asia Pacific Pte. Ltd, Singapore • Tongji University, Shanghai, China • GAPS Network, Environment and Climate Change Canada, Government of Canada – Air Quality Research Division, Science and Technology Branch
Local
• Institute of Environmental Science and Meteorology, UP Diliman • Institute of Chemistry, UP Diliman • Institute of Biology, UP Diliman • National Graduate School of Engineering, UP Diliman • Benguet State University • Department of Agriculture – Bureau of Agricultural Research • Philippine Trade and Training Center Department of Education – Bureau of Curriculum Development • Laguna Lake Development Authority (LLDA) • Professional Regulation Commission (PRC) • Integrated Chemist of the Philippines
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Other activities of the RASL in 2018
Three-Day Planning Workshop on the Topic “Familiarization of the ISO 17025:2017 Standard and Research Dissemination and Environmental Exposure Trip”, RASL Training Room and Baguio City, June 21-23, 2018.
Fig. 34. RASL staff during the Research Dissemination and Environmental Exposure Trip at Benguet State University, La, Trinidad, Benguet, June 22- 23, 2018.
Fig. 35. Research Dissemination at Benguet State University on June 22, 2018
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Fig. 36. Dr. Charita S. Kwan (3rd from the right) with Ms. Helen Subradil, trainor and groupmates in the DTI-PTTC training on Documentation of Laboratory Management System (Based on ISO/IEC 17025:2017) on November 6-7, 2018.
Fig. 37. Ms. Jonah L. Bondoc (2nd from left, back row) at the Tongji University in Shanghai, China for the 2018 2018 Asia-Pacific Leadership Programme, September 2018.
Fig. 38. Ms. Kristine O. Manalang at the Shimadzu Asia Pacific Pte. Ltd, with UNU participants and Shimadzu Laboratory Manager and staff, Singapore on November 5-9, 2018.
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Where regulations are concerned, the RASL with support from the NSRI Administration, strives to comply with the rules imposed by regulatory bodies (e.g., DENR-EMB, PDEA, PNP) on the use of regulated chemicals and disposal of chemical wastes. Two of the four permanent REPS of the RASL have been designated as the Pollution Control Officers of the NSRI in compliance with the requirements of DENR- EMB for all entities discharging or emitting substances.
Manpower Development
The RASL is basically composed of five permanent REPS, two UP contractual REPS (one of them is assigned as the chemical safety and waste management associate of the NSRI), one non-UP contractual research fellow, two permanent administrative personnel and one non-UP contractual utility worker.
Cognizant of the University’s academic stature, the REPS of the RASL are expected to pursue graduate studies preferably in chemistry or in other allied disciplines. Further, the RASL staff are guided by the core values of honesty, integrity and service to country and the Filipino people.
Activities of RASL Staff in 2018
1) CHARITA S. KWAN (University Extension Specialist V)
Positions held
• Laboratory Head • Member, NSRI Safety Committee • Affiliate Faculty, UPD IESM • Member, a Committee of the OVCAA • National Project Coordinator (Philippines), United Nations University – Institute for the Advanced Study of Sustainability funded project on “Monitoring and Management of Persistent Organic Pollutants in Asia”
Publications and Papers Presented (Please see section on RASL Publications and Papers Presented)
Professional/Extension Activities
• Member. Integrated Chemists of the Philippines • Member. Metrology, Standard, Testing and Quality (MSTQ), Inc. • Technical Expert Witness, Office of the Solicitor General for a case with the RASL test results as evidence – meetings. January 16, 17, 18, 19, 2018. • Invited Lecturer: "Persistent Organic Pollutants in the Marine Environment” in Marine Pollution Chemistry Class of Dr. Gil Jacinto. March 20, 2018 and November 15, 2018. • Trainor-Lecturer. Training Course on “Quality Assurance in Chemical Analysis”, UP NSRI Conference Room, UP Diliman, Quezon City. April 4-6, 2018 (1st Batch) and July 18-20, 2018 (2nd Batch). • Lead Organizer. Three-Day Planning Workshop on the topic, “Familiarization of the ISO 17025:2017 Standard and Research Dissemination and Environmental Exposure Trip”, RASL Training Room and Baguio City. June 21-23, 2018. • Speaker. Research Dissemination Activity at the Benguet State University. La Trinidad, Benguet, City. June 22, 2018.
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• Training Coordinator. The Mid-year 2018 Chemistry 197 (Special Topics in Applied Chemistry – Practicum of 200 hours) - Internship Program on Chemical Analysis and Instrumentation. July 2, 2018 to August 3, 2018. • Exploratory meetings for possible research collaborations: Dr. Elena Catap of IB. August 22, 2018; Ms. Celia Balbin of NISMED. October 4, 2018. • Trainor-Lecturer. Training Course on “Validation of Chemical Methods of Analysis”, UP NSRI Conference Room, UP Diliman, Quezon City. August 29-31, 2018. • Invited Resource Speaker: Dep-ED-Bureau of Curriculum Development, Curriculum Standards Development Division: Consultative Meeting on Math and Science Equipment, Mandaluyong City. October 9 and 11, 2018. • Trainor-Lecturer. On-site Training Course on “Validation of Chemical Methods of Analysis” for the Staff of the Philippine Associated Smelting and Refining (PASAR) Corporation in Leyte Industrial Development Estate (LIDE), Isabel, Leyte. October 23-25, 2018. • Panel Member. Defense of dissertation proposal of Mr. Renato Agustin, PhD Environmental Engineering candidate, UP Diliman College of Engineering. August 4, 2018. • Co-Adviser. Colloquium of Jonah L. Bondoc, PhD Env. Science candidate. June 18, 2018. • Co-Adviser. Defense dissertation proposal of Ms. Aileen Castaneda, PhD Environmental Engineering candidate, UP Diliman College of Engineering. June 30, 2018. • Co-Adviser. Defense dissertation proposal of Ms. Maria Teresa Marbella Mina, PhD Environmental Engineering candidate, UP Diliman College of Engineering. August 4, 2018. • Co-Adviser. Defense of MS thesis proposal of Mr. Kenneth Arguelles, MS Environmental Engineering candidate, UP Diliman College of Engineering. December 3, 2018
Seminars/Symposia/Trainings attended
• Updates on the Chemistry Profession Act: A Chemistry Week 2018 Symposium, The Integrated Chemists of the Philippines, Century Park Hotel, Manila. February 19, 2018. • Basic HPLC and Sample Preparation Workflow for LC/MS/MS, DKSH Philippines Inc. & Agilent Technologies, Research and Analytical Services Laboratory UP NSRI, Miranda Hall, UP Diliman, Quezon City. March 6, 2018. • 27th Symposium on Environmental Chemistry and the UNU-IAS Project Meeting, Okinawa, Japan. May 22-24, 2018. • REPS General Assembly, NISMED, UP Diliman. September 25, 2018. • University of the Philippines Systemwide REPS Conference, School of Statistics Auditorium and NISMED, UP Diliman. October 18-19, 2018. • ICP-OES and XRF Spectrometric Analysis by Dr. Preciosa Corazon B. Pabroa, Philippine Associated Smelting and Refining (PASAR) Corporation in Leyte Industrial Development Estate (LIDE), Isabel, Leyte. October 24, 2018. • DTI-PTTC training, “Documentation of Laboratory Management System (Based on ISO/IEC 17025:2017)”, Philippine Trade and Training Center, PTTC Bldg. Sen. Gil J. Puyat Ave. cor. Roxas Blvd., Pasay City. November 6-7, 2018. • Chemical Safety and Chemical Waste Management, Research and Analytical Services Laboratory, UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. December 6, 2018.
2) JONAH L. BONDOC (University Researcher II)
Positions held
• Designated Deputy Manager for Research and Trainings, Analyst/Supervisor • Designated 2018 NSRI PBB Focal Person
Publications and Papers Presented (Please see section on RASL Publications and Papers Presented)
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On-going PhD studies
• Completed 29 units and on-going dissertation on “Polychlorinated Biphenyls (PCBs) in Cetaceans Found Stranded in Philippines Waters – Linking Contamination Levels, Toxic Equivalents and Histopathological Results” for PhD in Environmental Science, UPD Institute of Environmental Science.
Professional/Extension activities
• Focal Person. Communications for updating media platforms of RASL with Ma. Cristine Faye J. Denna: RASL Website, Gmail account and registration links for the offered training courses, Facebook account, Bulletin board (re-started) and Brochure. • Trainor-Lecturer. Training Course on “Quality Assurance in Chemical Analysis”, UP NSRI Conference Room, UP Diliman, Quezon City. April 4-6, 2018 (1st Batch) and July 18-20, 2018 (2nd Batch). • Speaker. Research Dissemination Activity at the Benguet State University, Baguio City. June 21-23, 2018. • Lead Trainor. Russel Dane T. Terrado and Samantha Angela B. Valdez, BS Chemistry seniors of Institute of Chemistry, UP Diliman, Quezon City, Chemistry 197 (Special Topics in Applied Chemistry – Practicum). Practical Training in Chemical Analysis and Instrumentation for the Mid-year 2018 On-the-Job Training. July 2 to August 3, 2018 and July 2-31, 2018, respectively. • Trainor-Lecturer. Training Course on “Validation of Chemical Methods of Analysis”, UP NSRI Conference Room, UP Diliman, Quezon City. August 29-31, 2018. • Resource Speaker. 6th Philippine Marine Mammal Stranding Network National Symposium, The Oriental Hotel, Palo, Leyte. October 5, 2018. • Trainor-Lecturer. Seminar/Workshop on “Chemical Safety and Chemical Waste Management”, UP NSRI Conference Room, UP Diliman, Quezon City. December 6-7, 2018. • Trainor. Adriel John Ilagan On-the-Job Training Sample Preparation for Trace Metals and Mercury.
Seminars/Symposia/Trainings attended
• Basic HPLC and Sample Preparation Workflow for LC/MS/MS, DKSH Philippines Inc. & Agilent Technologies, Research and Analytical Services Laboratory UP NSRI, Miranda Hall, UP Diliman, Quezon City. March 6, 2018. • The Overview on Procurement Modes and Introduction of New Process and Template for Small Value Procurement, UP NSRI / UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. April 17, 2018. • Awareness Seminar on Transition from ISO/IEC 17025:2005 to ISO/IEC:2017, Philippine Trade and Training Center, PTTC Bldg. Sen. Gil J. Puyat Ave. cor. Roxas Blvd., Pasay City. May 18, 2018. • 33rd Philippine Chemistry Congress, The Integrated Chemists of the Philippines, Philippine International Convention Center, Manila. May 30 to June 1, 2018. • Asia-Pacific Leadership Programme on Environment for Sustainable Development, UN Environment-Tongji Institute of Environment for Sustainable Development Tongji University / Shanghai, China. September 17-21, 2018.
3) KRISTINE O. MANALANG (University Researcher I)
Positions held
• Designated Quality Officer and Analyst/Supervisor • Pollution Control Officer, NSRI
Publications and Papers Presented (Please see section on RASL Publications and Papers Presented)
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Professional/Extension activities
• Member, NSRI Special Events Committee • Speaker, “Chemical Safety and Chemical Waste Management” In: NSRI Safety Seminar “Safety and Security in the Workplace: A Primer on NSRI’s Safety and Security Rules and Guidelines”, NSRI. February 23, 2018. • In-charge, Chemical waste management plan of the RASL in its application for the certification to operate a chemical laboratory from the PRC-BOC. • Trainor-Lecturer. Training Course on “Quality Assurance in Chemical Analysis”, UP NSRI Conference Room, UP Diliman, Quezon City. April 4-6, 2018 (1st Batch) and July 18-20, 2018 (2nd Batch). • Speaker. Research Dissemination Activity at the Benguet State University. Baguio City. June 21-23, 2018. • Trainor. Russel Dane T. Terrado and Samantha Angela B. Valdez, BS Chemistry seniors of Institute of Chemistry, UP Diliman, Quezon City, Chemistry 197 (Special Topics in Applied Chemistry – Practicum). Practical Training in Chemical Analysis and Instrumentation for the Mid-year 2018 On-the-Job Training. July 2 to August 3, 2018 and July 2-31, 2018, respectively. • Trainor-Lecturer. Seminar/Workshop on “Chemical Safety and Chemical Waste Management”, UP NSRI Conference Room, UP Diliman, Quezon City. December 6-7, 2018. • In-charge. Participation of RASL in the Proficiency Testing (PT) water sample: preparation of stock samples, arsenic analysis and preparation of measurement uncertainty (MU) forms.
On-going MS Studies
• Earned 29 units for MS Chemistry, UPD Institute of Chemistry
Seminars/Symposia/Trainings attended
• Basic HPLC and Sample Preparation Workflow for LC/MS/MS, DKSH Philippines Inc. & Agilent Technologies, Research and Analytical Services Laboratory UP NSRI, Miranda Hall, UP Diliman, Quezon City. March 6, 2018. • Analysis Session on Analytical Method for Perfluorochemicals (PFCs): UNU-IAS Shimadzu Project on Monitoring and Management of POPs in Asia, Chonnam National University, Yeosu, South Korea. March 20-23, 2018. • The Overview on Procurement Modes and Introduction of New Process and Template for Small Value Procurement, UP NSRI / UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. April 17, 2018. • 27th JEC Symposium, Okinawa, Japan. May 22-25, 2018. • 33rd Philippine Chemistry Congress, Philippine International Convention Center, Manila. May 30 to June 1, 2018. • Orientation to the University of the Philippines Anti-Sexual Harassment Code 2017, UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. June 13, 2018 • ISO/IEC 17025:2017 The Standard for Laboratory Competence, Philippine Trade and Training Center, PTTC Bldg. Sen. Gil J. Puyat Ave. cor. Roxas Blvd., Pasay City. October 30, 2018. • Analysis Session on Analytical Method for Perfluorochemicals (PFCs): UNU-IAS Shimadzu Project on Monitoring and Management of POPs in Asia, Shimadzu Asia Pacific Pte. Ltd, Singapore. November 5-9, 2018
4) VERGEL G. VALENZUELA (University Researcher I)
Positions held
Chemical Safety and Waste Management Associate, NSRI (January – March 2018) University Researcher I, NSRI (March – present)
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Analyst/Supervisor
Publications and Papers Presented (Please see section on RASL Publications and Papers Presented)
Professional/Extension activities
• Speaker. “Chemical Safety and Chemical Waste Management” In: NSRI Safety Seminar “Safety and Security in the Workplace: A Primer on NSRI’s Safety and Security Rules and Guidelines”, NSRI. February 23, 2018. • Trainor-Lecturer. Training Course on “Quality Assurance in Chemical Analysis”, UP NSRI Conference Room, UP Diliman, Quezon City. April 4-6, 2018 (1st Batch) and July 18-20, 2018 (2nd Batch). • Speaker. Research Dissemination Activity at the Benguet State University. Baguio City. June 21-23, 2018. • Trainor. Russel Dane T. Terrado and Samantha Angela B. Valdez, BS Chemistry seniors of Institute of Chemistry, UP Diliman, Quezon City, Chemistry 197 (Special Topics in Applied Chemistry – Practicum). Practical Training in Chemical Analysis and Instrumentation for the Mid-year 2018 On-the-Job Training. July 2 to August 3, 2018 and July 2-31, 2018, respectively. • Trainor-Lecturer. Training Course on “Validation of Chemical Methods of Analysis”, UP NSRI Conference Room, UP Diliman, Quezon City. August 29-31, 2018. • Trainor-Lecturer. On-site Training Course on “Validation of Chemical Methods of Analysis” for the Staff of the Philippine Associated Smelting and Refining (PASAR) Corporation in Leyte Industrial Development Estate (LIDE), Isabel, Leyte. October 23-25, 2018. • Trainor-Lecturer. Seminar/Workshop on “Chemical Safety and Chemical Waste Management”, UP NSRI Conference Room, UP Diliman, Quezon City. December 6-7, 2018. • In-charge, Participation of RASL in the Proficiency Testing (PT) water sample: Sample preparation and instrumentation of Trace metals in water PT (18-103, Hg, Pb, Cd, Mn, Zn and Cr). • In-charge, Participation of the RASL in the Proficiency Testing (PT) program for the analysis of TOC in water leading to the Laboratory Excellence Recognition for the RASL by the Environment Resource Associates, U.S.A.
On-going MS Studies
• Earned 27 units for Master in Environment and Natural Resource Management, UP Open University.
Seminars/Symposia/Trainings attended
• Basic HPLC and Sample Preparation Workflow for LC/MS/MS, DKSH Philippines Inc. & Agilent Technologies, Research and Analytical Services Laboratory UP NSRI, Miranda Hall, UP Diliman, Quezon City. March 6, 2018. • 27th Symposium On Environmental Chemistry. Okinawa, Japan. May 22-25, 2018. • 33rd Philippine Chemistry Congress, Philippine International Convention Center, Manila. May 30 to June 1, 2018. • Orientation to the University of the Philippines Anti-Sexual Harassment Code 2017, UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. June 13, 2018. • Basic Training Course for Pollution Control Officers, Environmental Trainings Support Services Inc. and DENR-EMB, Bella Ibarra, Quezon Ave., West Triangle, Quezon City. July 10-14, 2018. • ISO/IEC 17025:2017 The Standard for Laboratory Competence, Philippine Trade and Training Center, PTTC Bldg. Sen. Gil J. Puyat Ave. cor. Roxas Blvd., Pasay City. 5 November 2018.
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5) ABEGAIL R. RESPONDE (University Research Associate II)
Positions held
• Designated Document Control Officer and Analyst/Supervisor • Member, NSRI Academic Personnel Committee (AcPC) • Member, Seminar Committee of NSRI Lecture Series
Professional/Extension activities
• Trainor-Lecturer. Training Course on “Quality Assurance in Chemical Analysis”, UP NSRI Conference Room, UP Diliman, Quezon City. April 4-6, 2018 (1st Batch) and July 18-20, 2018 (2nd Batch). • Speaker. Research Dissemination Activity at the Benguet State University. Baguio City. June 21-23, 2018. • Trainor. Russel Dane T. Terrado and Samantha Angela B. Valdez, BS Chemistry seniors of Institute of Chemistry, UP Diliman, Quezon City, Chemistry 197 (Special Topics in Applied Chemistry – Practicum). Practical Training in Chemical Analysis and Instrumentation for the Mid-year 2018 On-the-Job Training. July 2 to August 3, 2018 and July 2-31, 2018, respectively. • Trainor-Lecturer. Training Course on “Validation of Chemical Methods of Analysis”, UP NSRI Conference Room, UP Diliman, Quezon City. August 29-31, 2018. • Moderator and Participant. NSRI Lecture on “Probabilistic Graphical Models Framework and Applications” NSRI Conference Room, NSRI, Miranda Hall, UP Diliman, Quezon City. October 18, 2018.
Seminars/Symposia/Trainings attended
Basic HPLC and Sample Preparation Workflow for LC/MS/MS, DKSH Philippines Inc. & Agilent Technologies, Research and Analytical Services Laboratory UP NSRI, Miranda Hall, UP Diliman, Quezon City. March 6, 2018. The Overview on Procurement Modes and Introduction of New Process and Template for Small Value Procurement, UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. April 17, 2018. Orientation to the University of the Philippines Anti-Sexual Harassment Code 2017, UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. June 13, 2018. ISO/IEC 17025:2017 The Standard for Laboratory Competence, Philippine Trade and Training Center, PTTC Bldg. Sen. Gil J. Puyat Ave. cor. Roxas Blvd., Pasay City. November 5, 2018. Chemical Safety and Chemical Waste Management, Research and Analytical Services Laboratory / UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. December 6-7, 2018
6) JANNELLE O. CRISTOBAL (University Research Associate I)
Positions Held
• Analyst, UNU-IAS and Shimadzu funded project
Professional/Extension activities
• In-charge, maintenance and upkeep of the LC-MS/MS. • Analyst-in-charge, Participation of the RASL in the Proficiency Testing (PT) program for the analysis of TOC in water leading to the Laboratory Excellence Recognition for the RASL by the Environment Resource Associates, U.S.A.
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On-going MS Studies
• Earned 26 units for MS Food Science, College of Home Economics, UP Diliman.
Seminars/Symposia/Trainings attended
• On-the-Job Training on Total Organic Carbon in Water by Sparging Technique, Research and Analytical Services Laboratory, Miranda Hall, UP Diliman, Quezon City. April 4 to May 16, 2018. • Bukluran 2018: The 1st Diliman International Symposium in Food Science and Nutrition, College of Home Economics, Department of Food Science and Nutrition, UP College Home Economics, UP Diliman, Quezon City. October 17-19, 2018. • ISO/IEC 17025:2017 The Standard for Laboratory Competence, Philippine Trade and Training Center, PTTC Bldg. Sen. Gil J. Puyat Ave. cor. Roxas Blvd., Pasay City. October 30, 2018. • Chemical Safety and Chemical Waste Management, Research and Analytical Services Laboratory / UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. December 6-7, 2018.
8) ADRIEL JOHN R. ILAGAN (University Research Associate I)
Positions held
• Chemical Waste Management Associate, NSRI • Analyst
Professional/Extension activities
• Member. Integrated Chemists of the Philippines • Speaker. “Chemical Safety and Chemical Waste Management” In: NSRI Safety Seminar “Safety and Security in the Workplace: A Primer on NSRI’s Safety and Security Rules and Guidelines”, NSRI. 17 September 2018. • Assist in the preparation of documents for the Performance-Based Bonus (PBB) requirements • Assistance in the Training Course in Chemical Safety and Chemical Waste Management • Coordinate for 2019 RASL Environmental Exposure Trip – Payatas • Examiner. UPCAT 2019, UP Diliman, Quezon City. October 27 and 28, 2018
On-going MS Studies
• Earned 21 units for MS Chemistry, UPD Institute of Chemistry
Seminars/Symposia/Trainings attended
• On-the Job-Training on “Training: Ventilation Measurement of RASL Hoods” Research and Analytical Services Laboratory, Miranda Hall, UP Diliman, Quezon City. Date: July 5, 2018 and December 7, 2018. • Training Course on “Validation of Chemical Methods of Analysis”, Research and Analytical Services Laboratory / UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. August 29-31, 2018. • On-the Job-Training on “Sample Preparation of Water for Analysis of Mercury”, Research and Analytical Services Laboratory, Miranda Hall, UP Diliman, Quezon City. July 10 to September 10, 2018. • On-the Job-Training on “Sample Preparation of Water for Analysis of Metals”, Research and Analytical Services Laboratory, Miranda Hall, UP Diliman, Quezon City. July 10 to September 10, 2018.
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• Chemical Safety and Chemical Waste Management, Research and Analytical Services Laboratory, UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. December 6-7, 2018.
8) AIRA SACHA NADINE S. FERRER (Research Fellow, CHED-PCARI Collaborative Project)
On-going MS Studies
Earned 16 units for MS Environmental Science, UPD Institute of Environmental Science and Meteorology.
Seminars/Symposia/Trainings attended
• Basic HPLC and Sample Preparation Workflow for LC/MS/MS, DKSH Philippines Inc. & Agilent Technologies, Research and Analytical Services Laboratory UP NSRI, Miranda Hall, UP Diliman, Quezon City. March 6, 2018. • Training Course on “Quality Assurance in Chemical Analysis”, Research and Analytical Services Laboratory / UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. July 18-20, 2018. • Training Course on “Validation of Chemical Methods of Analysis”, Research and Analytical Services Laboratory / UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. August 29-31, 2018.
9) MA. CRISTINE FAYE J. DENNA (University Research Associate I, Special Project)
On-going MS Studies
• Earned 23 units for MS Environmental Science, UPD Institute of Environmental Science and Meteorology
Seminars/Symposia/Trainings attended
• Basic HPLC and Sample Preparation Workflow for LC/MS/MS, DKSH Philippines Inc. & Agilent Technologies, Research and Analytical Services Laboratory UP NSRI, Miranda Hall, UP Diliman, Quezon City. March 6, 2018.
Special Project
• Assistant. Development of media platforms of RASL: RASL Website, Gmail account and registration links for the offered training courses, Facebook account, Bulletin board (re- started) and Brochure.
10) JENALYN V. CRUZ (Administrative Assistant II)
Positions held
• Designated Laboratory Secretary
On-going MS Studies
• Earned 24 units for Master of Public Administration UPD NCPAG
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Seminars/Symposia/Trainings attended
• The Overview on Procurement Modes and Introduction of New Process and Template for Small Value Procurement, UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. April 17, 2018. • Orientation to the University of the Philippines Anti-Sexual Harassment Code 2017, UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. June 13, 2018. • ISO/IEC 17025:2017 The Standard for Laboratory Competence, Philippine Trade and Training Center, PTTC Bldg. Sen. Gil J. Puyat Ave. cor. Roxas Blvd., Pasay City. October 30, 2018. • Chemical Safety and Chemical Waste Management, Research and Analytical Services Laboratory, UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. December 6-7, 2018. • Examiner. UPCAT 2019, UP Diliman, Quezon City. October 27 and 28, 2018
11) CECILIA B. MENGUIS (Laboratory Technician I)
Seminars/Symposia/Trainings attended
• Chemical Safety and Chemical Waste Management, Research and Analytical Services Laboratory, UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. December 6-7, 2018
12) PATRICK R. PESTILOS (Utility Worker, January 2 – October 31, 2018)
Seminars/Symposia/Trainings attended
• Orientation to the University of the Philippines Anti-Sexual Harassment Code 2017, UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. June 13, 2018
2.2 INFORMATION SERVICE
The NSRI provides information on its technical services and research activities through the following publications (for details, please see section 1.2).
1) The NSRI Research and Extension Services 2) The NSRI Catalog of Cultures 3) The NSRI Brochure 4) Announcement of Training Courses (uploaded in the NSRI Website @www.nsri.upd.edu.ph)
2.3 COMMUNITY SERVICE
The NSRI as a national center of excellence in the basic sciences strives to promote consciousness and appreciation for the natural sciences by hosting visits of students and researchers and accommodating requests for demonstration of modern equipment for various groups.
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2.4 BIOSAFETY PROGRAM
The NSRI continues to implement a Safety Program for the benefit of NSRI-based researchers. The program aims to orient NSRI-based research and laboratory personnel in the safety procedures implemented in the NSRI. The NSRI Biosafety Committee conducts the NSRI Biosafety Seminar twice a year. Attendance to the seminar is a prerequisite in working in the NSRI facilities. The Biosafety Committee is chaired by Dr. Maria Auxilia T. Siringan, who is the Biological Safety Officer of the Institute. The Pollution Control Officer who implements the Chemical Waste Management Program of the Institute is Ms. Kristine O. Manalang.
The NSRI is the only institute in the University of the Philippines Diliman which continuously implements a laboratory biosafety program, conducts a regular biosafety seminar and chemical waste management.
2.5 LECTURE SERIES
The following talks were hosted by the NSRI during its Lecture Series in 2018.
Title : The Intriguing SPIRALS: Links to Food Safety and Gut Health Speaker : Dr. Miguel M. Garcia, International Consultant o Food Safety and Microbiology (Ottawa) NAST Corresponding Member
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Title : Sexual Dimorphism During Development: Man vs. Woman on a Molecular Level Speaker : Dr. Lerrie Ann DG Ipulan-Colet Assisting Professor, Institute of Biology, UP Diliman Visiting Researcher, Institute of Advanced Medicine, Wakayama Medical University, Japan
Title : Probabilistic Graphical Models Framework and Applications Speaker : Dr. Reinabelle C. Reyes Professorial Lecturer, National Institute of Physics, UP Diliman
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Title : Finding Info @ www.cslib.upd.edu.ph Speaker : Ms. Antoinette D. Villaflor Head Librarian, College of Science Library UP Diliman
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3. ORGANIZATION AND PERSONNEL
Organization and Management
The Organizational Chart of both regular and contractual personnel of NSRI is shown in Fig. 39. The various units, their principal personnel and functions are briefly described below:
Fig. 39. The NSRI Organizational Chart
Executive Council
This is the highest policy-making authority within the NSRI consisting of the NSRI Director and the Dean of the College of Science as Co-Chairpersons and the representatives of the disciplinal and interdisciplinary units covered by the mandate of NSRI. The Executive Council evaluates all research proposals and progress reports as well as other requests pertaining to research.
The Director serves for a term of three years and is the chief executive of the Institute. He/she communicates and implements the decisions of the Executive Council to
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parties concerned, represents the Institute and/or negotiates collaborations on behalf of NSRI, initiates programs and taps external sources of funding, both national and international.
The representatives are selected by their respective units for a term of two years. They supervise the screening of research proposals within their units and act as liaison of their units with the Executive Council.
The following were the members of the Executive Council in 2018:
Chair: Dr. Windell L. Rivera Director, NSRI
Co-Chair: Dr. Perry S. Ong (January 2, 2018 – December 31, 2018) Dean, College of Science
Members:
Dr. Jonathan A. Anticamara (January 1, 2018 – July 31, 2018) Dr. Michael C. Velarde (August 1, 2018 – December 31, 2018) Institute of Biology
Dr. Guillermo M. Nuesca – (August 1, 2018 – December 31, 2018) Institute of Chemistry
Dr. Joseph Manuel C. Loquias Department of Mathematics
Dr. Gay Jane P. Perez (January 2, 2018 – December 31, 2018) Institute of Environmental Science and Meteorology
Dr. Elmer S. Estacio Material Science and Engineering Program
Research Section
This section performs the research functions of the NSRI. At present, most of the research is undertaken through the faculty of the College of Science in the following disciplines: Biology, Chemistry, Mathematics, and Environmental Science and Meteorology. The research thrusts in these areas therefore reflect those of the institutes. The members of NSRI’s regular REPS who were initially hired to provide technical services to researchers were recognized as a research unit in 1988 under the Research Policies and Guidelines of NSRI. As such, the NSRI REPS have also been active in soliciting grants from NSRI. NSRI’s REPS have also been active in obtaining external support for their projects (see section on Externally - Funded Researches).
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The research personnel of the NSRI thus consist of the following:
1. Project Leaders, Co-Project Leaders and Collaborators: faculty researchers of the College of Science, NSRI research staff and adjunct researchers of the College of Science. 2. Contractual Research Staff: University Research Associate I, hired on a contractual basis for a particular project.
Technical Support Section
This section provides technical services for researchers and performs the extension services described in Technical assistance and Advisory services section. The principal units are the Biological Research and Services Laboratory (BRSL), the DNA Analysis Laboratory (DAL), the Microbiological Research and Services Laboratory (MRSL) and the Research and Analytical Services Laboratory (RASL).
Biological Research and Services Laboratory (BRSL)
1 University Researcher III 1 University Researcher II 1 University Researcher I 2 University Research Associate I 2 University Research Associate I (UP contractual) 1 Administrative Aide V DNA Analytical Laboratory (DAL)
1 University Researcher V/Scientist II 1 University Researcher III/Scientist I 1 University Researcher III 1 University Researcher I 3 University Research Associate I 1 University Research Associate I (UP contractual) 1 Research Associate (non-UP contractual) 1 Consultant
Microbiological Research and Services Laboratory (MRSL)
1 University Extension Specialist IV 2 University Researcher II 1 University Researcher I 1 Laboratory Technician Il 1 University Research Associate I (UP contractual) 1 Administrative Aide V (UP contractual) 1 Administrative Aide II (UP contractual) 1 Administrative Aide I (UP contractual) 1 Laboratory Staff (non-UP contractual) 1 Consultant
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Research and Analytical Services Laboratory (RASL)
1 University Extension Specialist V 1 University Researcher ll 2 University Researcher I 1 University Research Associate II 2 University Research Associate I (UP contractual) 1 Research and Analytical Staff (non-UP contractual) 1 Laboratory Technician I 1 Administrative Assistant II
Administrative Support Section
This section consists of the staff of the Administrative Office, Publication Section and the Property Office:
1. Administrative Office: handles the administration of research projects and actively supports all activities of the in-house laboratories of the Institute. The specific functions are: (1) to provide assistance to researchers in financial management; (2) to provide support to clerical and artistic services in the preparation of research manuscripts; (3) to coordinate with different laboratories regarding their activities for research and extension activities, such as holding of seminars, training courses, etc. (4) to arrange schedules of use of Institute facilities; and (5) to take charge of tour visits of students and guests in the Institute. The staff consists of:
1 Administrative Officer V 1 Administrative Officer IV 1 Administrative Officer III 1 Administrative Assistant V 1 Administrative Assistant II 1 Administrative Aide IV 1 Administrative Aide III
2. Publication Section: offers printing, binding and photocopying services. It is equipped with necessary facilities and manpower for the publication of research reports, manuals, books, etc. The staff consists of:
1 Publication Circulation Officer II
3. Property Section: in-charge of the overall maintenance and upkeep of NSRI building and grounds, facilities and equipment, property and supply, administration of electrical, plumbing and carpentry work. The staff consists of:
1 Administrative Officer V 1 Precision Instrument Technician III 1 Administrative Assistant III 1 Administrative Aide Vl 1 Administrative Aide lV 1 Laboratory Aide II
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4. Computer Room: provides for the computer needs of researchers, URAs and administrative personnel, including e-mail and internet access provided by the University.
List of NSRI Room Occupants
Below is the complete list of NSRI room occupants in 2018.
Table 8. List of NSRI’s room occupants in 2018.
Name of NSRI room/Lab occupants Assigned rooms
Director’s Office Rm. 234
NSRI In-house Laboratories: Biological Research and Services Laboratory (BRSL) Rms. 118-127 + EM/DNA Lab DNA Analysis Laboratory (DAL) Rms. 201-212 (12 rooms), 222 & 226 Microbiological Research Services Laboratory (MRSL) Rms. 101 - 102 Research and Analytical Services Laboratory (RASL) Rms. 134-135, 138-139 and Pesticide Laboratory
NSRI Offices: Administration Office Rm. 234 Property & Maintenance Office Rm. 137 DA-BAR/UP-NSRI Project Office Rm.233 DA-BAR/UP-NSRI Researchers Room Rm.237 DA-BAR (Drying Room) Rm.223 DA-BAR temporary room w/ fume hood Rm.237 UP-NSRI/PCAMRD-DOST Project Room Rm.114
Provision for Property & Maintenance Section (PMS): Office Rms.239, 241, 243 Stock Room Rm.245 (PHEIRL)
NSRI Rooms: Chemical Waste Storage Rm.15 Conference/ Council Room Rm.231 Computer Room Rm.236 Guest Scientist Room Rm. 246 General Room for Use of Fume Hood/Storage Room Rm.247 General Room (Fume Hood, Hot Room, Sterilizer) Rms.235, 244 Dr. Maria Auxilia Siringan’s Project Rm.115 Dr. Wildell L. Rivera AH 7&9 (PHEIRL) Dr. Pierangeli G. Vital SR 1&3 (PHEIRL) Animal Facility SR 2&3 (PHEIRL) Animal House No room number Solvent Storage Room No room number Distillation Shed No room number Hot Room Rm.113
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Personnel Inventory of the NSRI
The list of regular personnel of the NSRI in 2018 is presented in Table 9. The total inventory of personnel for 2018 is provided in Table 10.
Table 9. Regular personnel of NSRI in 2018 STA- TUS NAME POSITION TITLE OF STATUS OF SERVICE APPT.
Windell L. Rivera Director Additional Assignment BIOLOGICAL RESEARCH AND SERVICES LABORATORY (BRSL) Pierangeli G. Vital University Researcher III T Temporary (eff. 08/01/18) Raquel O. Rubio University Researcher II P On active duty (eff. 04/04/18) Joy Ann P. Santos University Researcher I T Temporary (eff. 09/01/18) Erna Louise L. Elias University Research Assoc. I P On active duty Maria Genesis G. Magleo University Research Assoc. I UC UP Contractual (until 06/30/18) Aurora Ines J. Aguilar University Research Assoc. I UC UP Contractual (eff. 10/15/18) Jude Christian E. Francisco University Research Assoc. I UC UP Contractual (eff. 10/15/18) Ryan May T. Jalova Administrative Aide V P On active duty (eff. 03/19/18) DNA ANALYSIS LABORATORY (DAL) Maria Corazon A. De Ungria University Researcher V / Scientist II P On active duty Gayvelline C. Calacal University Researcher II / Scientist I P On active duty Frederick C. Delfin University Researcher III P On active duty (eff. 02/23/18) Minerva S. Sagum University Research Assoc. I P On active duty Jazelyn M. Salvador University Researcher I P On active duty (eff. 10/16/18) Miriam Ruth M. Dalet University Research Assoc. I P On active duty (eff. 02/19/18) Paul Ryan L. Sales University Research Assoc. I UC UP Contractual Nelvie Fatima Jane A. Soliven University Research Assoc. I UC UP Contractual Bea G. Gallardo Research Associate NUC Non-UP Contractual MICROBIOLOGICAL RESEARCH AND SERVICES LABORATORY (MRSL) Maria Auxilia T. Siringan University Extension Specialist IV P On active duty Celia A. Cereno University Researcher II P On active duty (eff. 04/04/18) Vina B. Argayosa University Researcher II P On active duty (eff. 04/04/18) Mary Ann Cielo R. San Diego University Researcher I P On active duty (eff. 09/03/18) Jamelle Justine C. Cataag University Research Assoc. I UC UP Contractual Reynaldo E. Javate, Jr. Laboratory Technician II P On active duty Rosalyn F. Diaz Admin Aide V UC UP Contractual (eff. 01/03/18) Ma. Adele N. Arjona Admin Aide II UC UP Contractual (eff. 01/03/18) Dionisio R. Villanueva Admin Aide I UC UP Contractual (eff. 01/03/18) Raquel Timola Utility Worker UC UP Contractual (eff. 01/03/18) RESEARCH AND ANALYTICAL SERVICES LABORATORY (RASL) Charita S. Kwan University Extension Specialist V P On active duty Jonah L. Bondoc University Researcher ll P On active duty (eff. 02/23/18) Abegail R. Responde University Research Associate II P On active duty Cecilia B. Menguis Laboratory Technician I P On active duty Jenalyn V. Cruz Administrative Assistant II P On active duty (eff. 02/01/18)
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Cont. of Table 9. Kristine O. Manalang University Researcher I P On active duty Janelle O. Cristobal University Research Associate I UC UP Contractual (eff. 09/18/17) Vergel Valenzuela Chem. Safety & Waste Mgt. Associate T Temporary Adriel John R. Ilaga University Research Associate I UC UP Contractual (eff. 07/03/18) ADMINISTRATIVE SECTION Leahfe P. Gabriel Administrative Officer V P On active duty Eleazar S. Del Remedio Administrative Officer IV P On active duty Edeza M. Rivera Administrative Officer III P On active duty Dimpna N. Bulaon Administrative Assistant V P On active duty Paul Anthony D. Vicente Administrative Assistant II T Temporary (eff. 03/01/18) Romeo M. Magtibay Administrative Aide III P On active duty Joseph S. Balucos Administrative Aide IV P On active duty PUBLICATION SECTION Vilma S. Vega Publication Circulation Officer II P On-active duty PROPERTY SECTION Alvin DC. Mallari Administrative Officer V P On-active duty Delfa B. Lancara Administrative Assistant III P On-active duty Maritess S. De Jesus Administrative Aide VI P On-active duty (eff. 03/01/18) Edgardo C. Gayanes Laboratory Aide II P On-active duty Joselito M. Mejia Precision Instrument Technician T On-active duty (eff. 07/09/18)
P – Permanent UC- Contractual T-Temporary NUC – Non-UP Contractual
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Table 10. Personnel inventory of NSRI in 2018 No. of Personnel CATEGORY (Man Years) TOTAL Regular Items Lumpsum
NON-REGULAR
Members, Executive Council 7 Research Section
Project Leaders 19 Co-Project Leaders 3 Program Coordinators 0 Collaborators 1 University Research Associate I 30 Administrative Aide V 1 Administrative Aide II 1 Administrative Aide I 1
REGULAR
Director (Professor 6) 1
Research Staff
University Researcher V/Scientist II 1 1 University Extension Specialist V 1 1 University Extension Specialist IV 1 1 University Researcher III/Scientist l 1 1 University Researcher III 2 2 University Researcher II 4 4 University Researcher I 5 5 University Research Associate II 1 1 University Research Associate I 5 5 Laboratory Technician II 1 1 Laboratory Technician I 2 2 Laboratory Aide II 1 1
Administrative Staff
Administrative Officer V 2 2 Publication Circulation Officer II 1 1 Administrative Officer IV 1 1 Administrative Officer III 1 1 Precision Instrument Technician III 1 1 Administrative Assistant V 1 1 Administrative Assistant III 1 1 Administrative Assistant II 2 2 Administrative Aide VI 1 1 Administrative Aide V 1 1 Administrative Aide IV 2 2 Administrative Aide III 1 1 ------TOTAL 104
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3.1 NSRI Staff Development in 2018
1) WINDELL L. RIVERA, Ph.D. (Director)
Positions held
Professor 6, Institute of Biology UP Scientist III, UP System, 2018-2020 Chair, Philippine Academy of Microbiology, 2018-2019 Vice President, Outstanding Young Scientists, Inc., 2018-2020 PRO, Phi Sigma Biological Sciences Honor Society, Alpha Chi Chapter Board Member, Institute of Biology Alumni Association, 2015-2019
Publications
Espiritu, C.L.A., Justo, C.A.C., Rubio, M.J., Svobodova, M., Bashammakh A.S., Alyoubi, A.O., Rivera W.L., Rollon, A.P., O' Sullivan, C.K. 2018. Aptamer Selection Against a Trichomonas vaginalis Adhesion Protein for Diagnostic Applications. ACS Infectious Diseases. DOI 10.1021/acsinfecdis.8b00065 Adao, D.E.V., Rivera, W.L. 2018. Recent Advances in Blastocystis sp. Research. Philippine Science Letters. 11(1): 39-50. Justo, C.A.C., Relucio-San Diego, M.A.C.V., Rivera, W.L. 2018. Metronidazole Susceptibility and TVV-infection of Trichomonas vaginalis from Metro Manila and Angeles City, Philippines. Acta Medica Philippina. 52(6): 553-556. Salas, R.L., Garcia, J.K.D.L., Miranda, A.C.R., Rivera, W.L., Nella, R.B., Sabido, P.M.G. 2018. Effects of Truncation of the Peptide Chain on the Secondary Structure and Bioactivities of Palmitoylated Anoplin. Peptides. 104 (2018) 7-14. Vital, P.G., Zara, E.S., Paraoan, C.E.M., Dimasupil, M.A.Z., Abello, J.J.M., Santos, I.T.G., Rivera, W.L. 2018. Antibiotic Resistance and Extended-spectrum Beta- lactamase Production of Escherichia coli Isolated from Irrigation Waters in Selected Urban Farms in Metro Manila, Philippines. Water. 2018. 10, 548. DOI 10.3390/w10050548. Valle, D.L., Jr., Cabal, A.B.S., Paclibare, P.A.P., Rivera, W.L. 2018. Enteroaggregative Escherichia coli Infections Among Children in a Tertiary Hospital in the Philippines. Southeast Asian Journal of Tropical Medicine and Public Health. 49 (3): 409-420. Mathay, A.C.M.T.A., Dimasupil, M.A.Z., Rivera, W.L., Vital, P.G.. 2018. Bacteriological Screening of Fresh and Fresh-cut Fruits Vended in Sselect Open Air Open Air Markets and Supermarkets in Metro Manila, Philippines. Philippine Science Letters. 11(Supplement): 37-42. Justo, C.A.C., Mapile, M.R.F., Santos, P.D.M., Rivera, W.L. 2018. Determination of the Optimal Pre-enrichment Period for the detection of Salmonella enterica in Artificially Inoculated Meat Products Using Culture, PCR and LAMP Assays. Philippines Science Letters. 11(Supplementary): 43-50. Obusan, M.C.M., Aragones, L.V., Rivera, W.L., Siringan, M.A.T. 2018. Antibiotic Susceptibility Patterns of Bacteria Isolated from Cetaceans Stranded in the Philippines. Aquatic Mammals. DOI 10.1578/AM.44.5.2018.
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2) LEAHFE P. GABRIEL (Administrative Officer V)
Additional assignments
Member, NSRI Research and Administrative Committee (RAC) Member, NSRI Administrative Personnel Committee (APC) Member, UP Supervisors Association, Inc.
Seminars/Trainings attended
Science Career System Orientation for Finance & Administrative Personnel of Various Government Agencies with CS Scientists, Hotel Jen, Pasay City. July 31, 2018. UP System Procurement on APP and PPMP Preparation and Walkthrough on Procurement Process, SOLAIR, UP Campus, Diliman, Q.C. July 27, 2018. Orientation to the University of the Philippines Anti-Sexual Harassment Code 2017, UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. June 13, 2018. Orientation Seminar on CSC 2017 Omnibus Rules on Appointments and Other Human Resource Actions (ORA-OHRA) and 2017 Rules on Administrative Cases in the CSC (RACCS), sponsored by UPSSAI, CS Auditorium, U.P. Diliman, Q.C. June 7, 2018. Data Privacy Act of 2012 Seminar Workshop, CS Auditorium, U.P. Diliman, Q.C. May 18, 2018. Rules on Administrative Cases in the Civil Service (2017 RACCS), NEC, U.P. Diliman, Q.C. April 24, 2018. Omnibus Rules on Appointment and Other Human Resource ActIons (ORA-OHRA), College of Mass Communication, U.P. Diliman, Q.C. April 23, 2018. Overview on Procurement Modes and Introduction of New Process and Template for Small Value Procurement by Ms. Rosalyn Joan Pascual, BAC Secretariat, U.P. Diliman, Q.C. April 17, 2018.
3) ALVIN DC. MALLARI (Adminstrative Officer V)
Additional assignment
• NSRI-APC member, Supervisory level • Supervisor – Property and Maintenance Section • Member - Union Representative Committee
Seminars attended
“Risk Management in Purchasing Process”. Yana Chemodities, 151 Kaliraya St., Q.C. October 23, 2018. Institute of Integrated Electrical Engrs., 5th Metro Manila Regional Conference, 5/f Mega B, Megatrade Hall 3, SM Mega Mall, Mandaluyong City. August 10-11, 2018. Resource person. NSRI Safety Seminar “Safety and Security in the Workplace: A Primer on NSRI’s Safety and Security Rules and Guidelines”, NSRI. February 23, 2018.
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4) VILMA S. VEGA (Publication Circulation Officer II)
Additional assignment
Member, UP Supervisors Association, Inc. Member, NSRI Seminar and Special Events Committee
Seminars attended
Orientation to the University of the Philippines Anti-Sexual Harassment Code 2017, UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. June 13, 2018. Data Privacy Act of 2012 Seminar Workshop, CS Auditorium, U.P. Diliman, Q.C. May 18, 2018.
5) EDEZA M. RIVERA (Administrative Officer III)
Additional Assignment
Representative, Non-Supervisory Level, NSRI AdPC. July 2015 – present
Seminars attended
Leave Administration Course for Effectiveness- CSC. National Engineering Center, U.P. Diliman, Q.C. April 10 – 11, 2018. The Overview on Procurement Modes and Introduction of New Process and Template for Small Value Procurement, UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. April 17, 2018. Data Privacy Act of 2012 Seminar Workshop, CS Auditorium, U.P. Diliman, Q.C. May 18, 2018. Orientation Seminar on Civil Service Commission 2017 Omnibus Rules on Appointments and Other Human Resource Actions (ORA-OHRA) - UPSSA, Inc. College of Science, U.P. Diliman, Q.C. June 7, 2018. Orientation to the University of the Philippines Anti-Sexual Harassment Code 2017, UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. June 13, 2018.
6) DIMPNA N. BULAON (Administrative Assistant II)
Additional Assignment
Representative, Non-Supervisory Level, NSRI AdPC. July 2016 – June 2019. Proctor. UPCAT 2019, UP Diliman, Quezon City. October 27 and 28, 2018.
Seminars attended
• Science Career System Orientation for Finance & Administrative Personnel of Various Government Agencies with CS Scientists, Hotel Jen, Pasay City. July 31,. 2018. • Orientation to the University of the Philippines Anti-Sexual Harassment Code 2017, UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. June 13, 2018.
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• Data Privacy Act of 2012 Seminar Workshop, CS Auditorium, U.P. Diliman, Q.C. May 18, 2018. • The Overview on Procurement Modes and Introduction of New Process and Template for Small Value Procurement, UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. April 17, 2018.
7) DELFA B. LANCARA (Administrative Assistant II)
Additional Assignment
• Member. UP Credit Cooperative • Member. UP Provident • Member. All UP Workers Union • Proctor. UPCAT 2019, UP Diliman, Quezon City. October 27 and 28, 2018.
Seminars attended
UP System Procurement on APP and PPMP Preparation and Walkthrough on Procurement Process, SOLAIR, UP Campus, Diliman, Q.C. July 20, 2018. Data Privacy Act of 2012 Seminar Workshop, CS Auditorium, U.P. Diliman, Q.C. May 18, 2018. Orientation to the University of the Philippines Anti-Sexual Harassment Code 2017, UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. June 13, 2018.
8) MARITESS C. DE JESUS (Administrative Aide IV)
Seminars attended
UP System Procurement on APP and PPMP Preparation and Walkthrough on Procurement Process, SOLAIR, UP Campus, Diliman, Q.C. July 20, 2018. Data Privacy Act of 2012 Seminar Workshop, CS Auditorium, U.P. Diliman, Q.C. May 18, 2018. Orientation to the University of the Philippines Anti-Sexual Harassment Code 2017, UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. June 13, 2018. The Overview on Procurement Modes and Introduction of New Process and Template for Small Value Procurement, UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. April 17, 2018.
9) PAUL ANTHONY D. VICENTE (Laboratory Technician I)
Seminars attended
UP System Procurement on APP and PPMP Preparation and Walkthrough on Procurement Process, SOLAIR, UP Campus, Diliman, Q.C. July 27, 2018. Orientation to the University of the Philippines Anti-Sexual Harassment Code 2017, UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. June 13, 2018. The Overview on Procurement Modes and Introduction of New Process and Template for Small Value Procurement, UP NSRI, Conference Room, Miranda Hall, UP Diliman, Quezon City. April 17, 2018.
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3.2 Resource Generation and Allocation
The NSRI’s resources are derived mainly from the U.P. Budget. Additional resources are generated from external grants to the NSRI research staff and the funds from technical extension services. A brief description of each source and their corresponding allocation is given below:
NSRI Budget
The NSRI’s Internal Operating Budget for 2018 is shown in Table 12.
The NSRI budget from the U.P. revolving fund is shown in Table 13. It showed how much allocation was given to research grants by discipline, how much went to MOOE, honoraria and URA’s salaries.
Funds from Technical Extension Services
The funds from technical extension services go to the UP Trust Account. It is used mainly to purchase minor equipment and additional supplies for the research and service laboratories as well as photocopying units. Also, it is used to hire non-UP contractual personnel as needed, and to provide incentives for the regular staff. The NSRI makes available through these services the expertise of its staff and the use of its maintained scientific equipment. The extension services fill a strong need of outside agencies, particularly medium level business and government laboratories with inadequate facilities and for specialized services like analytical services, microbiological services, etc. The technical extension services provide a great opportunity for the University of the Philippines to fulfill a critical national need and at the same time generate funds for the Institute and the University.
3.3 Fiscal Resources
Financial assistance amounting to P 3 million was received by NSRI from the office of the Chancellor in 2018 as additional grant for Research MOOE.
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Table 12. The NSRI’s Internal Operating Budget in 2018 Appropriation Particulars Allocation Reserve Withheld Total
FOR: RESEARCH GRANTS (Current Operating Expenditures)
I. Personnel Services 1. Permanent Positions a. Number b. Salary per PSI c. Salary difference Adjusted TOTAL 2. Lump sum for wages, contractuals, P 5,826,000 P 5,826,000 substitute, faculty, graduate student, assistants and consultants 3. Lump sum for lecturers, honoraria, allowances and hazard pay 1,810,000 1,810,000 4. PERA/ACA/Bonus/Cash Gift 5. Magna Carta 6. GSIS life & ret., Medicare, ECIP TOTAL FOR PS 7,636,000 Appropriation Particulars Allocation Reserve Withheld Total II. Maintenance & Other Operating Expenses (MOOE) 1. Regular 4,331,000 4,331,000 2. Utilities 3. Janitorial 4. UP Provident Fund HMO TOTAL MOOE 4,331,000
TOTAL APPROPRIATIONS FOR RESEARCH GRANTS 11,967,000
FOR: REGULAR GRANTS (Current Operating Expenditures)
I. Personnel Services 1. Permanent Positions a. Number 28 28 b. Salary per PSI 11,154,000 11,154,000 c. Salary Difference Adjusted TOTAL 2. Lump sum for wages, contractuals, substitute, faculty, graduate student, assistants and consultants 3. Lump sum for lecturers, honoraria, allowances and hazard pay 116,000 116,000 4. RATA, PERA,ACA, Clothing, PEI, Mid-year & Year-End Bonus, Cash Gift, Magna 3,003,000 3,003,000 Carta 6. GSIS,PhilHealth,ECIP,PAGIBIG,RLIP 1,507,000 1,507,000
TOTAL FOR PS 15,780,000 II. Maintenance & Other Operating Expenses (MOOE) 1. Regular 576,000 576,000 2. Utilities 3. Janitorial/Guard 4. UP Provident Fund HMO TOTAL MOOE 576,000
TOTAL APPROPRIATIONS FOR REGULAR GRANTS 16,356,000
GRAND TOTAL APPROPRIATIONS P 28,323,000
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FUTURE PLANS AND RECOMMENDATIONS
Research. While the NSRI is known for its extension services, focus on its research programs will be equally pursued. The Institute will continue to partner with government agencies like the Department of Science and Technology (DOST) and the Department of Agriculture (DA) for research funding opportunities. In-house laboratories will continue to establish linkages with international scientists and experts within and outside UP to update their research capabilities. These will result to an increase in the number of international publications generated from these collaborative researches.
Manpower. The NSRI will continue to request for additional REPS items from UP Diliman administration to support the research and extension services of the in-house laboratories. It is also the aim of the Institute to increase the number of REPS recognized as Scientists either in the UP Scientific Productivity System or in the Scientific Career System jointly organized by the DOST and Civil Service Commission (CSC).
The administrative staff will continue to attend workshops related to their duties and responsibilities in the Institute. Doing so will improve the quality of their support to research and other activities of the Institute.
Infrastructure. The Institute will continue to seek help from the University to upgrade its facilities.
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WINDELL L. RIVERA, PH.D.
MS. VILMA S. VEGA
MS. RAQUEL O. RUBIO MS. JOY ANN P. SANTOS
(632) 928-6868 / (632) 925-2963
Miranda Hall corner Quirino and Velasquez Streets., UP Campus, Diliman, Quezon City 1101 [email protected]