Chemical Constituents from Acer mandshuricum Bull. Korean Chem. Soc. 2010, Vol. 31, No. 4 929 DOI 10.5012/bkcs.2010.31.04.929 Chemical Constituents from Acer mandshuricum and Their Effects on the Function of Osteoblastic MC3T3-E1 Cells Yan Ding, Chun Liang, Huu Tung Nguyen, Eun Mi Choi, Jeong Ah Kim, and Young Ho Kim* College of Pharmacy, Chungnam National University, Daejeon 305-764, Korea. *E-mail:
[email protected] Received October 26, 2009, Accepted February 16, 2010 A new compound, 4-methoxyl 5-hydroxymethyl benzoic 3-O-β-D-glucopyranoside (1), has been isolated from the leav- es and stems of Acer mandshuricum, along with nine known compounds (2-10). Their structures were determined by a variety of spectroscopic analyses. The effect of compounds 1-10 on the function of osteoblastic MC3T3-E1 cells was examined by determining alkaline phosphatase (ALP) activity, collagen synthesis, and mineralization. Compound 1 significantly increased the function of osteoblastic MC3T3-E1 cells; 5.0 µM of 1 increased ALP activity, collagen syn- thesis, and mineralization of MC3T3-E1 cells to 114.7, 119.5, and 108.2% (P < 0.05) of the basal value, respectively. In addition, compounds 2-10 also potently increased the function of osteoblastic MC3T3-E1 cells. Key Words: Acer mandshuricum, Aceraceae, 4-Methoxyl 5-hydroxymethyl benzoic 3-O-β-D-glucopyrano- side, Osteoblast differentiation, MC3T3-E1 cell Introduction GC spectra: Shidmazu-2010 spectrometer. Column chromato- graphy (CC): silica gel 60 (40 - 63 and 63 - 200 µm particle size, Osteoporosis is a major health concern for aging communiti- Merck) and RP-18 (40 - 63 µm particle size, Merck).