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High Diversity of Testate Amoebae (Amoebozoa, Arcellinida) Detected by HTS Analyses in a New England Fen Using Newly-Designed Taxon-Specific Primers

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High Diversity of Testate Amoebae (Amoebozoa, Arcellinida) Detected by HTS Analyses in a New England Fen Using Newly-Designed Taxon-Specific Primers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    Journal of Academia and Industrial Research (JAIR) Volume 3, Issue 3 August 2014 139 ISSN: 2278-5213 RESEARCH ARTICLE Protozoan Fauna of Freshwater Habitats in South Dum Dum Municipality, North 24 Parganas, West Bengal J. Chitra Protozoology Section, Lower Invertebrate Division, M Block, New Alipore, Kolkata-700053, India [email protected]; +91 98315 47265 ______________________________________________________________________________________________ Abstract Wetlands of South Dum Dum Municipality were focused to reveal the status of the planktonic protozoan fauna in detail. A total of 37 different sites were selected and plankton samples from these sites were collected. About 16 sp. of protozoa were identified from few localities from the present investigation. Eight species of rhizopoda belonged to 4 genera, 4 family (Pelomyxidae, Arcellidae, Centropyxidae and Difflugiidae) and 2 order (Pelobintida and Arcellinida), Four species of flagellate belongs to 2 genera, 1 family (Euglinidae) and 1 order (Euglenida), 4 species of ciliate belongs to 4 genera, 4 family (Colepidae, Vorticellidae, Euplotidae and Paramaeciidae), 2 order (Prorodontida and Peritrichida) and 2 suborder (Sporadotrichinia and Peniculina). Among 37 localities, protozoans were observed only in L2, L3, L8, L9, L12, L13, L15, L17, L18, L19, L21, L24, L26, L32, L33, L34 and L36 localities. Protozoan diversity and their abundance were noticed higher in L12, L18, L21, L26, L33 and L34 localities. Euglena viridis, E. acus, E. oxyuris and Phacus acumininata, Pelomyxa palustris, Vorticella companula were found to be higher in abundance and distribution. Keywords: South Dum Dum municipality, planktonic protozoan, Euglena viridis, abundance, distribution. Introduction Dumdum Park, Amarpalli, Telipukur, Nager Bazar, Protozoa are highly abundant in all aquatic habitats and Patipukur and Dum Dum were selected and the plankton greatly involved in food chain (Finlay, 1997).
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    Protist, Vol. 162, 545–570, October 2011 http://www.elsevier.de/protis Published online date 28 July 2011 PROTIST NEWS A Revised Classification of Naked Lobose Amoebae (Amoebozoa: Lobosa) Introduction together constitute the amoebozoan subphy- lum Lobosa, which never have cilia or flagella, Molecular evidence and an associated reevaluation whereas Variosea (as here revised) together with of morphology have recently considerably revised Mycetozoa and Archamoebea are now grouped our views on relationships among the higher-level as the subphylum Conosa, whose constituent groups of amoebae. First of all, establishing the lineages either have cilia or flagella or have lost phylum Amoebozoa grouped all lobose amoe- them secondarily (Cavalier-Smith 1998, 2009). boid protists, whether naked or testate, aerobic Figure 1 is a schematic tree showing amoebozoan or anaerobic, with the Mycetozoa and Archamoe- relationships deduced from both morphology and bea (Cavalier-Smith 1998), and separated them DNA sequences. from both the heterolobosean amoebae (Page and The first attempt to construct a congruent molec- Blanton 1985), now belonging in the phylum Per- ular and morphological system of Amoebozoa by colozoa - Cavalier-Smith and Nikolaev (2008), and Cavalier-Smith et al. (2004) was limited by the the filose amoebae that belong in other phyla lack of molecular data for many amoeboid taxa, (notably Cercozoa: Bass et al. 2009a; Howe et al. which were therefore classified solely on morpho- 2011). logical evidence. Smirnov et al. (2005) suggested The phylum Amoebozoa consists of naked and another system for naked lobose amoebae only; testate lobose amoebae (e.g. Amoeba, Vannella, this left taxa with no molecular data incertae sedis, Hartmannella, Acanthamoeba, Arcella, Difflugia), which limited its utility.
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  • Conicocassis, a New Genus of Arcellinina (Testate Lobose Amoebae)
    Palaeontologia Electronica palaeo-electronica.org Conicocassis, a new genus of Arcellinina (testate lobose amoebae) Nawaf A. Nasser and R. Timothy Patterson ABSTRACT Superfamily Arcellinina (informally known as thecamoebians or testate lobose amoebae) are a group of shelled benthic protists common in most Quaternary lacus- trine sediments. They are found worldwide, from the equator to the poles, living in a variety of fresh to brackish aquatic and terrestrial habitats. More than 130 arcellininid species and strains are ascribed to the genus Centropyxis Stein, 1857 within the family Centropyxidae Jung, 1942, which includes species that are distinguished by having a dorsoventral-oriented and flattened beret-like test (shell). Conicocassis, a new arcel- lininid genus of Centropyxidae differs from other genera of the family, specifically genus Centropyxis and its type species C. aculeata (Ehrenberg, 1932), by having a unique test comprised of two distinct components; a generally ovoid to subspherical, dorsoventral-oriented test body, with a pronounced asymmetrically positioned, funnel- like flange extending from a small circular aperture. The type species of the new genus, Conicocassis pontigulasiformis (Beyens et al., 1986) has previously been reported from peatlands in Germany, the Netherlands and Austria, as well as very wet mosses and aquatic environments in High Arctic regions of Europe and North America. The occurrence of the species in lacustrine environments in the central Northwest Ter- ritories extends the known geographic distribution of the genus in North America con- siderably southward. Nawaf A. Nasser. Department of Earth Sciences, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario, K1S 5B6, Canada. [email protected] R. Timothy Patterson.
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  • Testate Amoebae As a Hydrological Proxy for Reconstructing Water-Table
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    1 Arcellinida testate amoebae (Amoebozoa: Arcellinida): model of organisms for assessing microbial biogeography T h i e r r y J . H e g e r , E n r i q u e L a r a , E d w a r d A . D . M i t c h e l l 1 WSL, Swiss Federal Institute for Forest, Snow and Landscape Research, Ecosystem Boundaries Research Unit, Wetlands Research Group, Lausanne, Switzerland; Laboratory of Ecological Systems, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland; Department of Zoology and Animal Biology, University of Geneva,Switzerland; and Biodiversity Research Center, University of British Columbia, Vancouver, Canada 2 Institute of Biology, Laboratory of Soil Biology, University of Neuchâtel, Switzerland 7.1 Introduction Although widely recognised as essential participants in ecosystem processes and representing a signifi cant part of the Earth’s biodiversity (Clarholm, 1985 ; Corliss, 2002; Schröter et al., 2003 ; Falkowski et al., 2004 ), eukaryotic microorganisms are very poorly understood from evolutionary and biogeographic points of view. Major questions concerning the diversity and the distribution of protists remain 2 completely unresolved. Arcellinida testate amoebae are an excellent group from which to get insights into these questions because they are easy to collect, pre- sent in diff erent habitats and they build a shell of characteristic morphology that remains even after the organism’s death. In this group, both cosmopolitan and restricted distribution patterns have been documented. Some morphospecies such as Apodera vas(=Nebela vas) , Alocodera cockayni or the whole genus Certesella have been reported as one of the most convincing examples of heterotrophic protist s with restricted distributions (Foissner, 2006 ; Smith and Wilkinson, 2007 ; Smith et al., 2008 ).
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  • This Thesis Has Been Submitted in Fulfilment of the Requirements for a Postgraduate Degree (E.G
    This thesis has been submitted in fulfilment of the requirements for a postgraduate degree (e.g. PhD, MPhil, DClinPsychol) at the University of Edinburgh. Please note the following terms and conditions of use: This work is protected by copyright and other intellectual property rights, which are retained by the thesis author, unless otherwise stated. A copy can be downloaded for personal non-commercial research or study, without prior permission or charge. This thesis cannot be reproduced or quoted extensively from without first obtaining permission in writing from the author. The content must not be changed in any way or sold commercially in any format or medium without the formal permission of the author. When referring to this work, full bibliographic details including the author, title, awarding institution and date of the thesis must be given. Protein secretion and encystation in Acanthamoeba Alvaro de Obeso Fernández del Valle Doctor of Philosophy The University of Edinburgh 2018 Abstract Free-living amoebae (FLA) are protists of ubiquitous distribution characterised by their changing morphology and their crawling movements. They have no common phylogenetic origin but can be found in most protist evolutionary branches. Acanthamoeba is a common FLA that can be found worldwide and is capable of infecting humans. The main disease is a life altering infection of the cornea named Acanthamoeba keratitis. Additionally, Acanthamoeba has a close relationship to bacteria. Acanthamoeba feeds on bacteria. At the same time, some bacteria have adapted to survive inside Acanthamoeba and use it as transport or protection to increase survival. When conditions are adverse, Acanthamoeba is capable of differentiating into a protective cyst.
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  • Amoebozoa) Is Congruent with Test Size and Metabolism Type
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  • Vita: OR Anderson
    CURRICULUM VITA O. Roger Anderson [Updated May 2020] BIRTH DATE: August 4, 1937 OCCUPATION: Microbial Physiological Ecologist, Biologist, and Educator PROFESSIONAL RANK: Professor of Natural Sciences, Columbia University T. C., 1964-present Teachers College ; Department Chairman, 1974-1980, 1993-1996, 2000-2017 Senior Research Scientist (Adj.), Biology, 1967-present Lamont-Doherty Earth Observatory of Columbia University Faculty Member at Large, Columbia University Graduate School of Arts and Sciences. 1993-present DEGREES: Bachelor of Arts (Botany) Washington University, St. Louis 1959 Master of Arts (Biological Education) Washington University 1961 Doctorate (Biology and Education) Washington University 1964 PROFESSIONAL EXPERIENCE (TEACHING): 1963-64 Washington University, St. Louis 1964-67 Assistant Professor of Natural Sciences Columbia University, Teachers College 1968-70 Associate Professor of Natural Sciences Columbia University, Teachers College 1971- Professor of Natural Sciences Columbia University, Teachers College 1992-1993 College Research Coordinator, Teachers College. 1993-1996 Associate Director, Division of Instruction, T. C. OFFICES IN NATIONAL AND INTERNATIONAL ORGANIZATIONS: 1976 President, National Association for Res. Science Teaching (International) 1993-95 President, Columbia University Chapter Sigma Xi Honorary Scientific Society (National) 1995 President, International Society of Protistology (International) ----------------------------------------------------------------------------------------------------
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