Product Datasheet
Rabbit IgG Isotype Control NB810-56910-10mg
Unit Size: 10 mg Store at 4C.
Reviews: 6 Publications: 35
Protocols, Publications, Related Products, Reviews, Research Tools and Images at: www.novusbio.com/NB810-56910
Updated 10/25/2020 v.20.1
Earn rewards for product reviews and publications. Submit a publication at www.novusbio.com/publications Submit a review at www.novusbio.com/reviews/destination/NB810-56910 Page 1 of 4 v.20.1 Updated 10/25/2020 NB810-56910-10mg Rabbit IgG Isotype Control Product Information Unit Size 10 mg Concentration 5 mg/ml Storage Store at 4C. Clonality Polyclonal Preservative 0.09% Sodium Azide Isotype IgG Purity Ion exchange chromatography Buffer PBS. Product Description Description This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG antibody. Host Rabbit Gene ID 3500 Gene Symbol IGHG1 Species Rabbit Specificity/Sensitivity By immunoelectrophoresis the IgG was shown to 1) react with antiserum specific for rabbit IgG, 2) not react with antiserum specific for IgA or IgM, and 3) produce a single precipitin arc with antiserum against rabbit serum identical to that produced with anti-IgG antisera. Notes Protein concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Product Application Details Applications Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation Recommended Dilutions Western Blot 1:100 - 1:2000, Chromatin Immunoprecipitation 1:10 - 1:500, Flow Cytometry 1:10 - 1:1000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoprecipitation 1:10 - 1:500 Application Notes IP usage was reported in scientific literature (PMID: 23872598). ChIP usage was reported in scientific literature (PMID: 24324133). Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined. Use in IHC reported in secitific publication PMID: 32736983 Page 2 of 4 v.20.1 Updated 10/25/2020 Images Immunohistochemistry-Frozen: Rabbit IgG Isotype Control [NB810- 56910] - Mouse ear skin (aceton-fixed frozen section) stained with rabbit IgG isotype control, which was used as a negative control. Image from verified customer review.
Immunohistochemistry-Frozen: Rabbit IgG Isotype Control [NB810- 56910] - Mouse ear skin (aceton-fixed frozen section) stained with rabbit IgG isotype control, which was used as a negative control. Image from verified customer review. Page 3 of 4 v.20.1 Updated 10/25/2020 Publications Roberts JT, Porman AM, Johnson AM Identification of m6A residues at single-nucleotide resolution using eCLIP and an accessible custom analysis pipeline RNA (New York, N.Y.) Apr 1 2021 12:00AM [PMID: 33376190] (IP) Bisoendial R, Tabet F et al. Apolipoprotein A-I Limits the Negative Effect of Tumor Necrosis Factor on Lymphangiogenesis. Arterioscler Thromb Vasc Biol Jan 11 2015 12:00AM [PMID: 26359513] (IHC, Mouse) Qazi IH, Yang H, Wei S et al. Dietary selenium deficiency and supplementation differentially modulate the expression of two ER-resident selenoproteins (selenoprotein K and selenoprotein M) in the ovaries of aged mice: Preliminary data Reprod Biol Jul 28 2020 12:00AM [PMID: 32736983] (IHC) Porman A, Roberts J, Chrupcala M et al. A single N6-methyladenosine site in lncRNA HOTAIR regulates its function in breast cancer cells bioRxiv Jun 9 2020 12:00AM (IP) Bengts S, Shamoun L, Kunath A et al. Altered IL-32 Signaling in Abdominal Aortic Aneurysm J. Vasc. Res. May 20 2020 12:00AM [PMID: 32434199] (IHC) Ho JJD, Balukoff NC, Theodoridis PR et al. A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism Nat Commun May 29 2020 12:00AM [PMID: 32472050] (IP) Muschter D, Fleischhauer L, Taheri S et al. Sensory neuropeptides are required for bone and cartilage homeostasis in a murine destabilization-induced osteoarthritis model Bone Jan 8 2020 12:00AM [PMID: 31926346] (IHC-P, Mouse) Antalikova J, Secova P, Horovska L et al. Missing Information from the Estrogen Receptor Puzzle: Where Are They Localized in Bull Reproductive Tissues and Spermatozoa? Cells Jan 10 2020 12:00AM [PMID: 31936899] (ICC/IF, Bovine) Czarnek M, Bereta J Proteolytic Processing of Neuregulin 2 Mol. Neurobiol. Dec 14 2019 12:00AM [PMID: 31838721] (IHC) Das B, Pal B, Bhuyan R et al. MYC Regulates the HIF2 alpha Stemness Pathway via Nanog and Sox2 to Maintain Self-Renewal in Cancer Stem Cells versus Non-Stem Cancer Cells Cancer Res. Jul 2 2019 12:00AM [PMID: 31266772] (ChIP, Mouse, Human) Cansby E, Magnusson E, Nunez-Duran E et al. STK25 Regulates Cardiovascular Disease Progression in a Mouse Model of Hypercholesterolemia Arterioscler. Thromb. Vasc. Biol. Jun 21 2018 12:00AM [PMID: 29930001] (Rabbit) Ma XH, Piao SF, Dey S et al. Targeting ER stress-induced autophagy overcomes BRAF inhibitor resistance in melanoma. J Allergy Clin Immunol. [PMID: 24569374] (WB, PLA, Human)
Details: Citation using the Agarose Immobilized form of this antibody. More publications at http://www.novusbio.com/NB810-56910 Novus Biologicals USA Bio-Techne Canada 10730 E. Briarwood Avenue 21 Canmotor Ave Centennial, CO 80112 Toronto, ON M8Z 4E6 USA Canada Phone: 303.730.1950 Phone: 905.827.6400 Toll Free: 1.888.506.6887 Toll Free: 855.668.8722 Fax: 303.730.1966 Fax: 905.827.6402 [email protected] [email protected]
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Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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