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Rabbit IgG Isotype Control NB810-56910-10mg

Unit Size: 10 mg Store at 4C.

Reviews: 6 Publications: 35

Protocols, Publications, Related Products, Reviews, Research Tools and Images at: www.novusbio.com/NB810-56910

Updated 10/25/2020 v.20.1

Earn rewards for product reviews and publications. Submit a publication at www.novusbio.com/publications Submit a review at www.novusbio.com/reviews/destination/NB810-56910 Page 1 of 4 v.20.1 Updated 10/25/2020 NB810-56910-10mg Rabbit IgG Isotype Control Product Information Unit Size 10 mg Concentration 5 mg/ml Storage Store at 4C. Clonality Polyclonal Preservative 0.09% Sodium Azide Isotype IgG Purity Ion exchange chromatography Buffer PBS. Product Description Description This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG . Host Rabbit Gene ID 3500 Gene Symbol IGHG1 Species Rabbit Specificity/Sensitivity By immunoelectrophoresis the IgG was shown to 1) react with antiserum specific for rabbit IgG, 2) not react with antiserum specific for IgA or IgM, and 3) produce a single precipitin arc with antiserum against rabbit serum identical to that produced with anti-IgG antisera. Notes concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Product Application Details Applications Western Blot, Chromatin , Flow Cytometry, /, , Immunoprecipitation Recommended Dilutions Western Blot 1:100 - 1:2000, Chromatin Immunoprecipitation 1:10 - 1:500, Flow Cytometry 1:10 - 1:1000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoprecipitation 1:10 - 1:500 Application Notes IP usage was reported in scientific literature (PMID: 23872598). ChIP usage was reported in scientific literature (PMID: 24324133). Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined. Use in IHC reported in secitific publication PMID: 32736983 Page 2 of 4 v.20.1 Updated 10/25/2020 Images Immunohistochemistry-Frozen: Rabbit IgG Isotype Control [NB810- 56910] - Mouse ear skin (aceton-fixed frozen section) stained with rabbit IgG isotype control, which was used as a negative control. Image from verified customer review.

Immunohistochemistry-Frozen: Rabbit IgG Isotype Control [NB810- 56910] - Mouse ear skin (aceton-fixed frozen section) stained with rabbit IgG isotype control, which was used as a negative control. Image from verified customer review. Page 3 of 4 v.20.1 Updated 10/25/2020 Publications Roberts JT, Porman AM, Johnson AM Identification of m6A residues at single-nucleotide resolution using eCLIP and an accessible custom analysis pipeline RNA (New York, N.Y.) Apr 1 2021 12:00AM [PMID: 33376190] (IP) Bisoendial R, Tabet F et al. Apolipoprotein A-I Limits the Negative Effect of Tumor Necrosis Factor on Lymphangiogenesis. Arterioscler Thromb Vasc Biol Jan 11 2015 12:00AM [PMID: 26359513] (IHC, Mouse) Qazi IH, Yang H, Wei S et al. Dietary selenium deficiency and supplementation differentially modulate the expression of two ER-resident selenoproteins (selenoprotein K and selenoprotein M) in the ovaries of aged mice: Preliminary data Reprod Biol Jul 28 2020 12:00AM [PMID: 32736983] (IHC) Porman A, Roberts J, Chrupcala M et al. A single N6-methyladenosine site in lncRNA HOTAIR regulates its function in breast cancer cells bioRxiv Jun 9 2020 12:00AM (IP) Bengts S, Shamoun L, Kunath A et al. Altered IL-32 Signaling in Abdominal Aortic Aneurysm J. Vasc. Res. May 20 2020 12:00AM [PMID: 32434199] (IHC) Ho JJD, Balukoff NC, Theodoridis PR et al. A network of RNA-binding controls translation efficiency to activate anaerobic metabolism Nat Commun May 29 2020 12:00AM [PMID: 32472050] (IP) Muschter D, Fleischhauer L, Taheri S et al. Sensory neuropeptides are required for bone and cartilage homeostasis in a murine destabilization-induced osteoarthritis model Bone Jan 8 2020 12:00AM [PMID: 31926346] (IHC-P, Mouse) Antalikova J, Secova P, Horovska L et al. Missing Information from the Estrogen Receptor Puzzle: Where Are They Localized in Bull Reproductive Tissues and Spermatozoa? Cells Jan 10 2020 12:00AM [PMID: 31936899] (ICC/IF, Bovine) Czarnek M, Bereta J Proteolytic Processing of Neuregulin 2 Mol. Neurobiol. Dec 14 2019 12:00AM [PMID: 31838721] (IHC) Das B, Pal B, Bhuyan R et al. MYC Regulates the HIF2 alpha Stemness Pathway via Nanog and Sox2 to Maintain Self-Renewal in Cancer Stem Cells versus Non-Stem Cancer Cells Cancer Res. Jul 2 2019 12:00AM [PMID: 31266772] (ChIP, Mouse, Human) Cansby E, Magnusson E, Nunez-Duran E et al. STK25 Regulates Cardiovascular Disease Progression in a Mouse Model of Hypercholesterolemia Arterioscler. Thromb. Vasc. Biol. Jun 21 2018 12:00AM [PMID: 29930001] (Rabbit) Ma XH, Piao SF, Dey S et al. Targeting ER stress-induced autophagy overcomes BRAF inhibitor resistance in melanoma. J Allergy Clin Immunol. [PMID: 24569374] (WB, PLA, Human)

Details: Citation using the Immobilized form of this antibody. More publications at http://www.novusbio.com/NB810-56910 Novus Biologicals USA Bio-Techne Canada 10730 E. Briarwood Avenue 21 Canmotor Ave Centennial, CO 80112 Toronto, ON M8Z 4E6 USA Canada Phone: 303.730.1950 Phone: 905.827.6400 Toll Free: 1.888.506.6887 Toll Free: 855.668.8722 Fax: 303.730.1966 Fax: 905.827.6402 [email protected] [email protected]

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Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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