Supplementary material for Online Publication

Supplementary Table S1. Primers used in the study.

Gene Name of Sequence the primer Cyclin D1 Ccnd1-F 5’-CCGTCCATGCGGAAGATC-3’ Cyclin D1 Ccnd1-R 5’-ATGGCCAGCGGGAAGAC-3’ Ki-67 Mki67-F 5’-AGTCTCTTGGCACTCACAGC-3’ Ki-67 Mki67-R 5’-TTGTCCTCGGTGGCGTTATC-3’ Caspase9 Casp9-F 5’-ATGCAGGGTGCGCCTAGT-3’ Caspase9 Casp9-R 5’-GGTCTCAAGGTCTGTGACCAG-3’ Bcl2 Bcl2-F 5’-ACAGAACCGCAAGACAGGAG-3’ Bcl2 Bcl2-R 5’-GCAAGGAGGACTTGGGGTTT-3’ cMyc Cmyc-F 5’-GGTTTGGAAGAGGCTAGGGG-3’ cMyc Cmyc-R 5’-TAGCCGTGAATGAGGGTAGC-3’ β-actin Actb-F1 5’-GCCGTTCCGAAAGTTGCC-3’ β-actin Actb-R1 5’-AGCTGCAAAGAAGCTGTGCT-3’ β-actin Actb-F2 5’-TCCTCCTGAGCGCAAGTACTCT-3’ β-actin Actb-R2 5’-GCTGATCCACATCTGCTGGAA-3’ GAPDH Gapdh-F1 5’-AATGTGTCCGTCGTGGATCTGA-3’ GAPDH Gapdh-R1 5’-GATGCCTGCTTCACCACCTTCT-3’ GAPDH Gapdh-F2 5’-GAAGGTGAAGGTCGGAGTCA-3’ GAPDH Gapdh-R2 5’-GTTAAAAGCAGCCCTGGTGA-3’ Cdc23 Cdc23-F 5’-GGCTGCAATAGCAAGAAAGC-3’ Cdc23 Cdc23-R 5’-GTAAAGGCCGAATCCATCAA-3’ Olig1 Olig1-F 5’-AGCCAGAAGTCTTCGCATCC-3’ Olig1 Olig1-R 5’-GGTAGACACTGTTGGGGAGC-3’ Tbx3 Tbx3-F 5’-TGAAGAACAAGGCAGCCAGGAG-3’ Tbx3 Tbx3-R 5’-TAATGGTCCAACAGGCACACG-3’ Egr1 Egr1-F 5’-TATACTGGCCGCTTCTCCCT-3’ Egr1 Egr1-R 5’-AGAGGTCGGAGGATTGGTCA-3’ Cyp7a1 Cyp7a1-F 5’-CCTCCGGGCCTTCCTAAATC-3’ Cyp7a1 Cyp7a1-R 5’-CACTCGGTAGCAGAAGGCAT-3’ Mvd Mvd-F 5’-TACTGGGGGATGTCGAGTGT-3’ Mvd Mvd-R 5’-TAGCCCTTCAGAAACCAGCG-3’ Saa1 Saa1-F 5’-GCAGTGGCAAAGACCCCAAT-3’ Saa1 Saa1-R 5’-CCTAGGTAGGAAGAAGCCCAGA-3’ JunB JunB-F 5’-TGGGCACATCACCACTACAC-3’ JunB JunB-R 5’-TCTGGCTATGCAGTTCAGCC-3’ Hmgn1 Hmgn1wt-F 5’-CCCCGCGCCGCCACGATGCCCAAGAGGAAG-3’ Hmgn1 Hmgn1wt-R 5’-CCATCCGCGCTAACCTGCACGAGAAAGCAC-3’ Neo Hmgn1ko-R 5’-CGACTGCATCTGCTGGTTCTAAG-3’ Intergenic-1 IG1_F 5’-TGCCAGAGTTAACCTTGCTTG-3’ Intergenic-1 IG1_R 5’-CAGTATTCACCAGGCCATCA-3’ Intergenic-2 IG2_F 5’-GCAGCAGATGGCAAGTAATACTAAGAT-3’ Intergenic-2 IG2_R 5’-CCCTTATTCTCTGAGCATTAGACAGTTATA-3’ Olig1 DHS3 OL3_ F 5’-AAGCCATTTTAGCCTGACCA-3’ Olig1 DHS3 OL3_ R 5’-TTGCCAGAGAGGGTTATCGT-3’ Olig1 DHS2 OL2_ F 5’-CCGACGCCAAAGAGGAACAG-3’ Olig1 DHS2 OL2_ R 5’-GAGCTACCCAGCAGCAGGATG-3’ Olig1 DHS1 OL1_ F 5’-CCAAGCTCACGCCTCCATTG-3’ Olig1 DHS1 OL1_ R 5’-CGCCTGGGAAATCGCATAGTAC-3’

Supplementary Table S2. List of differentially expressed genes (FC>2, p≤0.01) in livers of Hmgn1+/+ and Hmgn1tm1/tm1 mice in groups A, B, and C.

The log2 expression ratios for individual genes is color-coded as shown below.

Higher expression ↔ lower expression 2 1 0 -1 -2

Hmgn1+/+ vs. Hmgn1+/+ vs. Hmgn1+/+ vs. Hmgn1 tm/tm1 Hmgn1 tm/tm1 Hmgn1 tm/tm1 Group: A B C Probe Set ID Gene Symbol 1415812_at Gsn 1.23 0.16 -0.60 1415822_at Scd2 0.67 -0.20 -0.60 1415885_at Chgb 0.45 -1.70 -1.66 1415899_at Junb -1.20 -0.21 -0.13 1415949_at Cpe 0.83 -0.89 -1.12 1416021_a_at Fabp5 /// Gm3601 1.15 0.70 -0.06 1416022_at Fabp5 1.51 0.81 -0.20 1416149_at Olig1 2.40 1.16 0.07 1416326_at Crip1 0.93 0.37 -0.22 1416454_s_at Acta2 1.61 0.58 -0.23 1416630_at Id3 -1.32 -0.72 -0.15 1416661_at Eif3a -0.14 0.02 -0.32 1416852_a_at Ncdn -0.48 0.05 -0.02 1416930_at Ly6d 0.34 -0.50 -0.64 1416955_at Slc25a10 0.44 0.34 0.06 1417023_a_at Fabp4 0.24 -1.01 -1.26 1417063_at C1qb 0.83 0.54 -0.08 1417065_at Egr1 -1.74 -0.98 -0.24 1417168_a_at Usp2 -0.72 0.11 0.59 1417273_at Pdk4 0.41 0.08 -0.76 1417303_at Mvd 1.52 0.64 0.03 1417370_at Tff3 0.05 0.09 0.90 1417408_at F3 -0.39 -0.18 0.21 1417600_at Slc15a2 -2.24 -1.20 -0.94 1417867_at Cfd 0.27 -0.35 -0.85 1417930_at Nab2 -1.02 -0.46 0.00 1417956_at Cidea -0.23 -0.29 -0.33 1417963_at Pltp 1.58 0.86 0.14 1418102_at Hes1 -1.64 -0.71 -0.14 1418149_at Chga 0.30 -0.87 -1.71 1418174_at Dbp -1.27 0.25 0.84 1418188_a_at Malat1 -0.13 -0.32 0.06 1418189_s_at Malat1 -0.15 -0.32 0.09 1418507_s_at Socs2 -1.18 -0.69 -0.57 1418511_at Dpt 1.21 0.54 -0.46 1418652_at Cxcl9 0.33 0.17 -0.28 1418654_at Hao2 0.13 -0.94 -1.52 1418726_a_at Tnnt2 0.77 0.18 -0.34 1418728_at Star -0.12 -2.08 -2.03 1418729_at Star 0.23 -1.23 -1.12 1418835_at Phlda1 -0.30 -0.04 0.22 1418918_at Igfbp1 -0.22 -0.17 -0.14 1418925_at Celsr1 0.72 0.56 0.02 1418932_at Nfil3 0.06 0.05 -0.42 1419075_s_at Saa1 -0.19 0.13 0.19 1419127_at Npy -0.10 -0.52 -0.81 1419154_at Tmprss2 -1.12 -0.66 0.15 1419247_at Rgs2 0.50 -0.14 -0.60 1419430_at Cyp26a1 -0.28 -0.11 -0.01 1419663_at Ogn 1.08 -0.08 -0.82 1419766_at Sik1 -1.03 -0.36 -0.20 1419820_at Pkhd1 0.96 0.44 -0.42 1419874_x_at Zbtb16 -0.36 -0.28 -0.22 1419918_at Tmed7 0.12 0.25 0.06 1419975_at Scp2 0.18 0.01 0.44 1420013_s_at Lss 1.04 0.62 -0.17 1420037_at LOC100503191 0.10 0.22 -0.06 1420089_at Nfkbia -0.05 0.08 -0.19 1420132_s_at Pttg1ip 0.00 0.14 0.09 1420438_at Orm2 0.65 0.34 0.11 1420650_at Zfhx3 -0.03 0.32 0.29 1420772_a_at Tsc22d3 0.51 0.44 -0.06 1420835_at Slc25a30 -0.58 0.27 0.30 1420836_at Slc25a30 -0.53 0.18 0.20 1420946_at Atrx 0.55 0.18 -0.75 1421037_at Npas2 0.39 0.14 -0.26 1421116_a_at Rtn4 0.33 0.17 -0.41 1421410_a_at Pstpip2 0.82 0.60 -0.15 1421447_at Onecut1 -0.34 0.33 0.60 Igh-2 /// Igh-VJ558 /// 1421653_a_at LOC544903 -0.12 0.03 0.05 1421679_a_at Cdkn1a -0.36 -0.21 0.04 1421818_at Bcl6 -1.62 -1.36 -0.41 1421874_a_at Mrps23 0.24 0.39 -0.36 1421977_at Mmp19 0.71 0.47 -0.09 1422100_at Cyp7a1 2.24 0.87 -0.90 1422198_a_at Shmt1 0.33 0.30 -0.30 1422557_s_at Mt1 -2.43 -0.77 0.70 1422651_at Adipoq -0.10 -1.69 -1.88 1422735_at Foxq1 -0.30 0.02 0.30 1422926_at Mc2r 0.12 -0.60 -1.11 1422973_a_at Thrsp 1.42 0.90 -0.01 1423319_at Hhex 1.48 0.79 0.19 1423381_at 4921521F21Rik 0.04 -0.93 -0.97 1423389_at Smad7 -1.38 -0.63 -0.11 1423397_at Ugt2b38 -0.89 0.02 0.42 1423418_at Fdps 1.08 0.65 -0.33 1423516_a_at Nid2 0.59 0.24 -0.39 1423547_at Lyz2 0.77 0.32 -0.28 1423556_at Akr1b7 0.32 -2.02 -1.95 1423828_at Fasn 1.20 0.91 0.19 1424126_at Alas1 0.56 0.37 0.14 1424262_at Aif1l -0.02 -0.24 0.19 1424351_at Wfdc2 1.05 0.80 0.21 1424607_a_at Gm4354 0.31 0.21 0.31 1424609_a_at Gm4354 0.41 0.19 0.27 1424638_at Cdkn1a -0.43 -0.21 -0.09 1424669_at Zfyve21 0.65 0.50 -0.31 1424673_at Clec2h 0.01 0.01 -0.37 1424737_at Thrsp 1.14 0.65 0.11 1424893_at Ndel1 0.05 0.10 0.42 1424932_at Egfr -1.36 -0.89 -0.32 1424942_a_at Myc 0.16 -0.07 -0.30 1424950_at Sox9 -0.81 -0.27 -0.33 1424967_x_at Tnnt2 1.12 0.30 0.01 1425099_a_at Arntl 0.12 -0.02 -0.44 1425222_x_at D630002G06Rik 0.92 0.57 -0.51 1425281_a_at Tsc22d3 0.75 0.70 0.04 1425296_a_at Rgs3 -0.16 0.06 0.19 1425329_a_at Cyb5r3 0.42 0.41 0.03 1425362_at Agfg2 -0.70 -0.22 0.24 1425424_at MGC7817 -0.20 0.00 0.15 1425673_at Lpp 0.05 0.05 0.06 1425701_a_at Rgs3 -0.25 -0.11 0.02 Igh-2 /// Igh-VJ558 /// 1425763_x_at LOC544903 -0.07 0.14 0.12 1425895_a_at Id1 -2.73 -1.55 -0.31 1426037_a_at Rgs16 1.08 1.01 0.25 1426278_at Ifi27l2a 0.39 -0.30 -0.99 1426389_at Camk1d 0.53 0.24 -0.33 1426663_s_at Slc45a3 0.12 0.11 -0.21 1426808_at Lgals3 0.57 0.14 -0.60 1426913_at 1.06 0.67 -0.35 BC005512 /// F630007L15Rik /// 1426936_at Gm6958 1.23 0.88 0.17 1426956_a_at Trp53bp1 0.41 0.12 -0.22 1427126_at Hspa1b 0.63 0.51 -0.22 1427127_x_at Hspa1b 0.52 0.50 -0.14 1427422_at Gm6484 -1.04 -0.58 0.02 1427520_a_at Myh1 0.05 0.54 1.18 1427747_a_at Lcn2 0.01 0.28 0.39 1427820_at 0.57 0.16 -0.17 1427826_a_at Slco1b2 0.00 -0.11 -0.52 1427838_at Tubb2a 0.37 0.08 0.08 1427868_x_at Myh1 -0.07 0.40 1.11 1427893_a_at Pmvk 1.07 0.60 -0.38 1427915_s_at LOC100045866 /// Tceb1 0.33 0.37 -0.28 1428021_at Mccc2 -0.29 0.10 -0.39 1428022_at Lcn13 0.16 0.52 0.51 1428070_at Syvn1 -0.56 -0.14 0.62 1428160_at Ndufab1 -0.02 0.21 -0.03 1428224_at Hnrpdl -0.02 -0.27 -0.21 1428306_at Ddit4 0.74 0.49 0.12 1428942_at Mt2 -3.12 -0.94 0.84 1429360_at Klf3 -0.17 -0.12 0.13 1429379_at Lyve1 -0.34 0.34 -0.04 1429786_a_at Zwint 0.51 0.28 -0.27 1430718_s_at Dnajc24 0.18 0.40 -0.41 1430838_x_at Mbd1 -1.02 -0.35 -0.24 1431056_a_at Lpl -0.08 -0.45 -1.15 1431182_at Hspa8 /// LOC624853 1.51 0.95 0.20 1431240_at Clec2h 0.08 0.15 -0.45 1431464_a_at Pmm2 0.20 0.32 -0.13 1431645_a_at Gdi2 -0.05 0.29 -0.27 1431721_a_at Proz 0.18 0.24 -0.06 1432344_a_at Aplp2 0.27 0.20 -0.42 1433443_a_at Hmgcs1 1.00 0.48 -0.29 1433446_at Hmgcs1 1.05 0.58 -0.36 1434087_at Mthfr 0.44 0.24 -0.19 1434099_at Ppargc1a 0.24 0.14 -0.32 1434272_at Cpeb2 -0.16 -0.41 -0.27 1434292_at Snhg11 0.99 0.54 -0.07 1434416_a_at Solh -0.01 0.11 0.25 1434465_x_at Vldlr 1.09 0.19 -0.09 1434739_at Fmr1nb -1.61 -1.12 -0.10 1435405_at Setd4 0.36 0.23 -0.03 1435458_at Pim1 -1.01 -0.53 -0.16 1435602_at 0.04 0.23 0.20 1435884_at Itsn1 0.57 0.33 0.26 1436186_at E2f8 -0.56 -0.04 -0.09 1436902_x_at Tmsb10 0.65 0.52 0.58 1436991_x_at Gsn 1.00 0.19 -0.35 1437171_x_at Gsn 1.18 0.17 -0.35 1437262_x_at Bcas2 0.03 -0.44 0.32 1437684_at Ftsjd1 -0.28 -0.41 0.08 1437729_at Gm7536 -0.03 0.35 -0.15 1437772_s_at Fuca1 0.28 0.25 -0.15 1437901_a_at Vps41 0.55 0.32 -0.15 1438069_a_at Rbm5 0.64 0.19 -0.12 1438156_x_at Cpt1a -0.17 -0.43 0.21 1438211_s_at Dbp -1.94 0.59 0.92 1438244_at Nfib 0.33 0.28 -0.31 1438245_at 0.30 0.28 0.50 1438397_a_at Rbm39 0.16 0.11 0.02 1438403_s_at Malat1 0.19 -0.16 0.10 1438511_a_at 1190002H23Rik -0.14 -1.27 -1.31 1438652_x_at Pigq 0.08 0.25 0.02 1438675_at Sfswap -0.71 -0.23 0.47 1438743_at Cyp7a1 2.56 1.20 -0.80 1438802_at Foxp1 0.13 0.25 -0.06 1438842_at Mtch2 -0.08 -0.05 0.17 1438953_at Figf 0.31 0.09 -0.03 1439264_x_at Lasp1 -0.17 -0.02 0.37 1439415_x_at Rps21 0.11 -0.08 0.36 1441115_at D18Ertd232e 0.73 0.26 -0.06 1442025_a_at -0.59 -0.44 -0.30 1442026_at -0.30 -0.23 -0.09 1442744_at Rbm39 0.03 0.10 0.13 1443949_at -0.06 0.05 0.12 1445689_at -0.50 -0.26 0.33 1446147_at Rbm39 -0.67 -0.25 0.17 1448021_at Fam46c -0.04 -0.03 -0.38 1448130_at Fdft1 1.01 0.70 -0.04 1448301_s_at Serpinb1a 1.07 0.40 -0.77 1448416_at Mgp 0.34 -0.54 -0.86 1448453_at Hsd3b1 0.06 -2.20 -2.15 1448507_at Efhd1 0.16 -0.44 -1.18 1448626_at Cdk5rap1 1.54 0.49 -0.45 1448663_s_at Mvd 1.30 1.04 0.06 1448666_s_at Tob2 -0.01 0.20 0.04 1448724_at Cish 0.55 0.32 0.04 1448804_at Cyp11a1 -0.12 -1.13 -1.62 1448950_at Il1r1 -1.12 -0.59 0.09 1449033_at Tnfrsf11b -0.11 0.10 -0.06 1449109_at Socs2 -0.98 -0.50 -0.53 1449145_a_at Cav1 0.63 -0.20 -0.47 1449164_at Cd68 0.99 0.63 -0.43 1449182_at Retn 0.03 -0.88 -0.88 1449201_at Star -0.14 -1.60 -1.99 1449326_x_at Saa2 -0.39 0.11 0.03 LOC100505359 /// Xlr4a 1449347_a_at /// Xlr4b /// Xlr4c 1.40 1.46 0.87 1449390_at Gpatch4 0.75 0.05 -0.37 1449676_at 0.35 0.11 -0.02 1449773_s_at Gadd45b -0.07 -0.35 -0.65 1449851_at Per1 -0.69 -0.19 0.31 1449854_at Nr0b2 -0.70 -0.43 -0.09 1450007_at 1500003O03Rik -0.04 0.14 -0.02 1450018_s_at Slc25a30 -0.42 0.12 0.11 1450051_at Atrx 0.05 -0.02 -0.53 1450252_at Onecut1 0.00 0.36 0.31 1450264_a_at Chka -0.28 -0.05 -0.16 1450708_at Scg2 0.29 -1.30 -1.78 1450788_at Saa1 -0.20 -0.05 0.29 1450792_at Tyrobp 1.02 0.46 -0.17 1451204_at Scara5 -3.53 -0.60 0.80 1451263_a_at Fabp4 0.13 -0.94 -1.07 1451438_s_at Clec2h 0.17 0.06 -0.51 1451557_at Tat -0.48 -0.17 -0.04 C730048C13Rik /// 1451635_at D630002G06Rik 1.74 0.66 0.11 1451799_at Ccdc25 1.32 0.94 0.23 1451956_a_at Sigmar1 0.39 0.26 -0.22 1452050_at Camk1d 0.46 0.30 -0.28 1452114_s_at Igfbp5 0.85 0.21 -0.18 1452318_a_at Hspa1b 0.47 0.25 0.08 1452416_at Il6ra -0.54 -0.27 -0.11 1452527_a_at P2rx4 -0.08 0.31 -0.18 1452670_at Myl9 1.27 0.51 -0.06 1452754_at Creld2 -0.45 0.36 1.02 1453596_at Id2 -2.15 -1.37 -0.47 1453740_a_at Ccnl2 0.15 0.02 0.08 1453988_a_at Ide 0.29 0.28 -0.26 1454838_s_at Pkdcc -0.24 -0.07 0.26 1455282_x_at Alas1 0.56 0.23 0.13 1455812_x_at Vasn -0.16 0.11 -0.06 1455938_x_at Rad21 -0.27 -0.33 0.10 1456037_x_at Preb 0.06 0.44 0.33 1456174_x_at Ndrg1 -0.14 -0.13 -0.68 1456292_a_at Vim 1.02 0.64 -0.21 1456319_at 1.38 0.47 -0.43 1456424_s_at Pltp 1.31 0.75 0.13 1456469_x_at Lasp1 -0.19 -0.40 -0.02 1460273_a_at Naip2 1.54 0.71 -0.02 1460368_at Mpp4 -0.81 -0.27 0.02 1460511_at Pkp2 -0.56 -0.22 0.05 1420136_a_at 0.02 0.17 -0.15 1428386_at Acsl3 0.90 0.70 0.02 1428670_at 1.03 0.59 -0.18 1428748_at Zfp826 0.47 0.27 0.17 1428923_at Ppp1r3g 1.23 0.88 -0.30 1429671_at Scand3 -0.60 -0.07 0.25 1429810_at Rictor -0.51 -0.46 -0.04 1429822_at 4633401B06Rik -0.27 -0.01 0.26 1429882_at 2610005L07Rik -0.36 0.03 0.28 1429900_at 5330406M23Rik 0.18 0.09 0.00 1430401_at 3110045C21Rik 1.22 0.64 -0.30 1430470_at 5730411F24Rik 0.60 0.36 -0.20 1430584_s_at Car3 1.07 0.79 0.03 1430622_at LOC100504504 -0.37 -0.17 0.06 1430637_at 2210016H18Rik 0.36 0.29 -0.24 1431101_a_at Srd5a1 -0.43 -0.07 -0.24 1431252_a_at Zfp655 -0.66 0.03 -0.05 1431316_at Itch 0.37 0.32 0.12 1431355_s_at Trpm7 -0.11 0.12 0.41 1431406_at Agxt2l1 0.49 0.38 0.06 1431528_at 5830427D02Rik -0.40 -0.08 0.48 1431806_at 4931408D14Rik 1.07 0.64 0.09 1432352_at Ccny 0.04 0.21 0.39 1432850_at 5430434G16Rik -0.24 -0.12 0.30 1433203_at 6030400A10Rik 0.82 0.77 0.23 1433266_at 2810416A17Rik -0.42 -0.06 0.23 1433453_a_at Abtb2 0.57 0.30 -0.23 1433454_at Abtb2 0.47 0.43 -0.14 1433632_at Irf2bp2 -0.21 -0.02 -0.41 1433634_at Irf2bp2 -0.39 -0.17 -0.23 1433898_at -0.65 -0.06 0.12 1434452_x_at Eif2a 0.15 -0.26 0.11 1435158_at Rbm12b 1.07 0.70 -0.03 1435319_at Ip6k2 0.50 0.37 -0.08 1435579_at -0.19 -0.08 0.45 1435595_at 1810011O10Rik 0.96 0.68 -0.16 1435888_at Egfr -1.60 -0.88 -0.25 1436200_at Lonrf3 -0.03 0.01 -0.64 1436202_at Malat1 0.24 -0.02 -0.50 1436453_at BB144871 0.13 -0.93 -0.93 1436497_at LOC100503785 0.35 0.56 0.36 1436631_at Ctnna1 -0.69 -0.03 0.18 1436660_at Rrbp1 -0.43 -0.24 -0.10 1436964_at D7Ertd715e 0.68 0.38 -0.30 1436968_x_at Klhl24 -0.05 0.09 -0.01 1437073_x_at -0.66 -0.17 0.16 1437129_at E330018D03Rik -0.56 -0.10 -0.07 1437162_at 0.11 0.08 0.05 1437285_at 1110020G09Rik 0.21 0.03 -0.05 1437584_at 0.10 0.12 -0.06 1437633_at Ankrd11 0.38 0.18 -0.22 1437669_x_at Ccrl1 0.36 0.30 -0.46 1437676_at Spag9 0.01 0.04 -0.26 1437821_at 0.26 0.11 0.10 1438072_at 0.39 0.16 0.04 1438259_at -0.25 -0.29 0.00 1438294_at Atxn1 -0.48 -0.08 0.26 1438331_at 0.50 0.39 0.02 1438345_at -0.33 -0.10 0.31 1438408_at Ankrd56 -1.04 -0.45 -0.11 1438487_s_at Zzz3 -0.18 -0.05 0.11 1438516_at Rif1 -1.10 -0.19 -0.14 1438543_at -0.09 0.12 0.01 1438558_x_at Foxq1 -0.21 0.06 0.31 1438643_at Camk1d 0.58 0.18 -0.31 1438664_at Prkar2b 0.01 -0.82 -1.12 1438719_at Map3k2 0.19 0.02 -0.27 1438788_at D5Wsu152e -0.32 0.01 0.41 1438862_at 0.56 0.19 -0.04 1438879_at 0.40 0.30 0.11 1438895_at A430102J17Rik -0.04 0.03 0.41 1438916_x_at 6720401G13Rik 0.73 0.33 0.07 1439123_at 0.00 -0.01 -0.11 1439163_at Zbtb16 -0.51 -0.42 -0.15 1439293_at BC031353 0.57 0.38 0.01 1439307_at 0.36 0.07 -0.13 1439331_at 4932439E07Rik -0.35 -0.14 0.04 1439342_at Clpx 1.26 0.87 -0.24 1439348_at S100a10 0.71 0.41 -0.01 1439353_x_at Tmem56 -0.12 -0.54 0.04 1439397_at Fmn1 1.02 0.66 -0.06 1439566_at Gprin3 -0.03 0.36 -0.01 1439617_s_at Pck1 -1.08 -0.28 -0.58 1439687_at Rab14 0.03 -0.03 0.22 1440068_at -0.71 -0.14 0.15 1440078_at -0.16 -0.11 0.35 1440163_at Setd3 -0.03 0.10 0.30 1440351_at 0.59 0.15 0.17 1440506_at 0.55 0.17 0.09 1440513_at C80258 -0.13 -0.05 0.26 1440624_at 0.86 0.78 0.06 1440660_at -1.15 -0.43 -0.10 1440671_at A130012E19Rik -0.42 0.10 0.27 1440749_at 0.24 0.24 0.12 1440790_x_at 0.22 0.18 0.24 1441108_at -0.26 0.05 0.28 1441231_at Gm3626 -0.63 -0.48 0.25 1441326_at Cp 0.31 0.28 -0.11 1441397_at -0.39 -0.08 0.11 1441413_at -0.39 -0.18 0.15 1441437_at -0.10 -0.20 -0.09 1441479_at 0.13 0.00 0.40 1441498_at 0.96 0.79 0.04 1441546_at Mpp6 -0.28 0.01 0.15 1441547_at 0.02 -0.02 -0.15 1441624_at Sorbs2 0.09 0.12 0.04 1441644_at 0.02 0.03 0.24 1441732_at -0.55 -0.19 0.19 1441769_at -0.55 -0.06 0.35 1441779_at 9530006C21Rik 0.01 0.00 0.25 1441823_at Zmiz1 0.00 0.01 0.07 1441871_at 1810044D09Rik -0.49 -0.26 -0.12 1441906_x_at Syap1 0.01 -0.23 0.30 1441926_x_at Tmie 0.96 0.57 -0.08 1441956_s_at Cux1 -0.50 -0.05 0.04 1442069_at Ept1 -0.27 -0.12 0.25 1442070_at Mllt10 -0.48 -0.03 0.05 1442277_at Chka -0.06 -0.29 -0.01 1442311_at Zfr -0.05 0.03 0.55 1442331_at 0.92 0.45 0.24 1442358_at AA409587 -0.08 0.12 0.46 1442381_at -0.33 -0.01 0.02 1442409_at D9Wsu90e 1.30 0.46 0.05 1442509_at 0.34 0.27 0.06 1442593_at 0.20 0.16 0.30 1442625_at -0.05 0.02 0.20 1442704_at -0.19 0.12 0.40 1442849_at Lrp1 -0.11 0.14 0.09 1442886_at 1.18 0.58 0.16 1442992_at 130004C03 -0.21 0.01 0.35 1443003_at 0.52 0.14 0.04 1443017_at Cpeb2 -0.19 -0.21 -0.63 1443056_at 1.27 0.81 0.24 1443075_at 0.36 0.10 0.27 1443086_at Alcam 0.11 -0.04 -0.10 1443088_at 9930031P18Rik -0.28 -0.09 0.14 1443109_at 0.32 0.26 -0.08 1443147_at 1.51 0.26 -1.24 1443206_at 0.01 -0.03 -0.02 1443231_at 0.03 0.06 0.05 1443327_at D130043K22Rik 1.28 0.77 -0.39 1443430_at 0.76 0.34 0.35 1443521_at -0.12 -0.10 0.27 1443526_at -0.27 -0.13 0.43 1443533_at 0.22 0.17 0.14 1443609_s_at Syvn1 -0.54 -0.19 0.71 1443624_at 0.19 0.25 0.14 1443789_x_at Cox8c -0.28 -0.67 0.00 1443923_at Akap13 0.70 -0.22 -0.20 1444032_at 0.16 0.61 0.63 1444036_at 0.84 0.43 -0.28 1444045_at -0.16 -0.14 0.49 1444114_at -0.11 -0.02 0.39 1444194_at -0.24 0.02 0.32 1444195_at 0.09 -0.04 0.26 1444268_at BC004004 -0.22 -0.07 -0.21 1444296_a_at Serpina4-ps1 -0.21 0.23 0.34 1444297_at Serpina4-ps1 -0.01 0.23 0.40 1444326_at 0.14 0.18 -0.37 1444349_at -0.25 0.06 0.44 1444473_at -0.04 0.19 0.18 1444518_at Acox1 -0.02 0.03 0.32 1444538_at -0.48 -0.28 0.30 1444622_at 0.73 0.37 -0.01 1445001_at -0.49 -0.19 0.06 1445225_at 2410001C21Rik -0.13 -0.23 -0.25 1445379_at -0.03 0.00 0.20 1445402_at -0.29 -0.09 -0.14 1445562_at 1.02 0.69 0.04 1445574_at -1.30 -0.48 0.05 1445676_at 1.13 0.57 -0.11 1445765_at 9430011C21Rik 0.42 0.38 0.18 1445980_at 1.00 0.57 0.26 1446007_at 0.56 0.26 0.07 1446068_at Adk 0.07 -0.02 0.09 1446127_at Zeb1 0.18 -0.09 0.26 1446230_at 0.66 0.31 -0.41 1446272_at Cdk17 0.46 0.17 0.15 1446284_at Mtss1 -0.01 0.25 0.63 1446461_at 0.12 -0.05 0.21 1446675_at Adk 0.47 -0.08 -0.09 1446850_at 0.08 0.25 0.37 1446990_at Nfia -0.30 -0.07 -0.15 1447023_at 0.23 0.08 -0.02 1447408_at -0.35 -0.27 0.17 1447517_at Skiv2l2 -1.77 -0.47 -0.20 1447560_at -0.16 -0.28 0.12 1447625_at E2f5 -0.02 -0.15 0.05 1447640_s_at Pbx3 -0.33 0.17 -0.04 1447657_s_at Synpo2l 0.00 -0.48 0.11 1447670_at Psmd9 -0.54 -0.10 0.11 1447719_at Apoh -0.17 -0.13 0.38 1447830_s_at Rgs2 0.27 0.11 -0.70 1447891_at 0.78 0.42 0.22 1447951_at 0.34 0.24 -0.09 1448092_x_at Serpina4-ps1 -0.35 0.17 0.38 1449746_s_at Glipr1 0.21 -0.09 0.03 1452678_a_at Ccbl1 0.55 0.38 -0.06 1452771_s_at Acsl3 0.66 0.67 -0.18 1452905_at Meg3 -0.01 -0.55 -0.93 1453103_at Ablim1 0.74 0.49 -0.32 1453345_at Nipal1 0.05 -0.04 -0.74 1453582_at Chka -0.43 -0.40 -0.16 1453588_at Car3 1.14 0.23 -0.27 1453595_at 2900064B18Rik 0.33 0.19 0.00 1453698_at 6030451C04Rik 0.15 0.47 0.13 1454766_at Amn1 1.14 0.63 -0.30 1455418_at 0.05 -0.01 -0.28 1455840_at Rapgef5 0.83 0.38 -0.25 1456159_at -0.24 -0.05 0.16 1456354_at Chrna4 1.00 0.15 -0.04 1456362_at Cyp11b1 -0.24 -1.45 -1.73 1456413_at Pde4dip -0.06 0.11 -0.03 1456446_at 4930523C07Rik -0.13 -0.16 0.45 1456475_s_at Prkar2b 0.16 -0.64 -0.98 1456514_at 0.06 -0.13 0.58 1456625_at Aasdhppt 0.05 0.13 -0.38 1456648_at 0.27 0.48 -0.18 1456661_at -0.39 0.00 -0.09 1456718_at Tmem56 -0.19 -0.48 0.11 1456742_x_at Tm9sf2 -0.41 -0.61 -0.12 1456864_at -0.09 0.26 -0.27 1456955_at 0.49 0.26 0.28 1456960_at -0.10 -0.04 0.15 1457178_at 0.22 0.39 0.78 1457264_at Phf20l1 -0.55 -0.23 0.19 1457374_at Nedd4l 0.23 0.17 -0.21 1457410_at Arhgap5 0.47 0.31 0.13 1457554_at Apob -0.01 -0.03 -0.16 1457670_s_at Lmna 0.28 0.51 -0.01 1457760_at A930004J17Rik 0.18 -0.03 0.26 1457851_at 0.04 0.01 0.30 1457944_at -0.47 -0.32 0.05 1458007_at Myo1b 0.54 -0.10 -0.09 1458069_at 0.28 0.28 0.40 1458099_at 0.22 0.07 0.27 1458205_at 0.61 0.31 0.33 1458344_at Nagpa -0.06 -0.18 0.25 1458347_s_at Tmprss2 -1.34 -0.72 0.01 1458578_at -0.77 -0.39 0.18 1458737_at C77097 0.17 0.03 -0.13 1458768_at C79946 -0.26 -0.17 0.15 1458974_at 0.56 0.60 0.15 1459008_at 0.01 0.02 0.30 1459196_at 0.23 0.19 -0.44 1459238_at 0.50 0.44 0.12 1459253_at 1700023H06Rik -1.33 -0.62 -0.05 1459344_at 9630019E01Rik -0.16 -0.34 -0.01 1459424_at D3Ertd108e 0.48 0.14 -0.16 1459557_at 0.46 0.20 0.04 1459635_at -0.12 0.19 0.05 1459861_s_at Kdm2b -0.53 -0.20 0.21 1459864_at Gpr146 -0.22 -0.39 0.25 1459879_at 4921513D23Rik 0.48 0.21 0.04 1459948_at 1.67 1.23 1.03 1459961_a_at Stat3 0.53 0.29 -0.21 1460049_s_at 1500015O10Rik 0.11 -0.89 -1.12

Supplementary Table S3. GO analysis of the differentially expressed genes

GO ACCESSION GO Term P value GO:0008203 cholesterol metabolic process 1.29E-06 GO:0016125 sterol metabolic process 2.39E-06 GO:0006695 cholesterol biosynthetic process 4.26E-06 negative regulation of sequence-specific DNA GO:0043433 binding transcription factor activity 1.00E-05 GO:0016126 sterol biosynthetic process 1.24E-05 negative regulation of transcription from RNA GO:0000122|GO:0010553|GO:0045816 polymerase II promoter 1.30E-05 GO:0001071 nucleic acid binding transcription factor activity 2.17E-05 sequence-specific DNA binding transcription GO:0003700|GO:0000130 factor activity 2.17E-05 regulation of transcription from RNA polymerase GO:0006357|GO:0006358|GO:0010551 II promoter 2.24E-05 regulation of cyclin-dependent protein kinase GO:0000079 activity 2.26E-05 GO:0048523|GO:0051243 negative regulation of cellular process 4.05E-05 GO:0008610 lipid biosynthetic process 4.09E-05 GO:0007623|GO:0050895 circadian rhythm 5.15E-05 GO:0048519|GO:0043118 negative regulation of biological process 5.95E-05 negative regulation of transcription, DNA- GO:0045892|GO:0016481|GO:0032582|GO:0061021 dependent 6.26E-05 GO:0007507|GO:0007511 heart development 6.42E-05 GO:0008299|GO:0009241 isoprenoid biosynthetic process 7.45E-05 GO:0051253 negative regulation of RNA metabolic process 7.94E-05