Revision: 2 Effective Date: 12/16/2019 temperature and atmosphere appropriate at the incubated Response: Cultural Expected Appearance: Prepared Appearance: Dehydrated Specifications Control Quality organisms. enteric of identification and using references toappropriate Refer Procedure Test 3. 2. 1. Preparation Refer Precaution specifications. performance to meet required as supplemented and/or adjusted be may Formula 25°C at 0.2 ± 7.4 Final pH: Agar Red Neutral Salts Bile Lactose Dige Enzymatic Casein of Digest Enzymatic Typical Formulation Mycobacterium allowing violet crystal not contain does medium this addition, de an to electrolyte provide the medium from Proteus of swarming that restricts medium a differential Salt is and Violet Crystal without Agar MacConkey colifo and pathogens enteric between reactions differential improved formula modifications The concentrations. red neutral and salts, bile altered content, agar chloride,decreased sodium 0.5% of addition the include modifications of growth improved modifications group. Formula coliform of strains differentiate wasto used medium MacConkey salt bile on the is based Agar MacConkey Description humans. in conditions other disease or of in diagnosis use the for of strains swarming containing specimens from bacilli enteric negative Salt and Violet Crystal without Agar MacConkey Use Intended Sheet Specification Technical
Autoclave at 121°C for 15 minutes. 15 for 121°C at Autoclave medium. the dissolve completely to minute one boil for and agitation frequent with Heat water. purified inof one liter the medium of 47 g Suspend
to SDS
MacConkeyAgarwithout Crystal Violet and Salt (NCM0072) spp., aiding in the detection and isolation of enteric microorganisms. Sodium Chloride is deleted deleted Chlorideis Sodium microorganisms. enteric of isolation and detection the in aiding spp.,
st of of st spp. to grow. to spp.
Gelatin rms. rms.
Prepared medium is trace to slightly hazy, and pink to rose pink and hazy, slightly to is trace medium Prepared
Powder is homogeneous, free flowing, and trace to light pink light to trace and flowing, free homogeneous, is Powder
Cultural response on MacConkey Agar MacConkey on response Cultural
MacConkey Agar without Crystal Violet and Salt for the isolation isolation the Salt for and Violet Crystal without Agar MacConkey
12 g/L0.04 10 1 1 - 5 0.0 0.0 neutral red neutral .0 .0 .0 ficient medium preventing preventing medium ficient
g/L g/L g/L g/L g/L is used for the isolation and differentiation of Gramof differentiation and the isolation for used is
- lactose agar of MacConkey. The original original The MacConkey. of agar lactose Salmonella typhosa typhosa Salmonella
Shigella and examined for growth after 18 after growth for examined and Staphylococcus
and and Proteus Proteus Proteus Salmonella Salmonella without from members of the of members from , Enterococcus, Enterococcus,
spp Crystal Violet & Salt & Violet Crystal spp. from spreading. In spreading. from spp. , and is not intended intended not is , and strains. These These strains. - orange. - beige.
- and 24
hours.
-
Revision: 2 Effective Date: 12/16/2019 light by and moisture from Protect storage temperature. at same the environment humidity low in a the container 2 at media culture dehydrated Store Storage 3. 2. 1. Procedure the of Limitations directed. as when stored container in its intact medium to applies Expiry color. the original from has changed appearance or if flowing, free st date toexpiration Refer Expiration reduced. bile. Non Lactose Results listed are organisms The Sheet Specification Technical Streptococcus pyogenes Streptococcus pneumonia Streptococcus aureus Staphylococcus sonnei Shigella typhimurium Salmonella mirabilis Proteus mirabilis Proteus coli Escherichia faecalis Enterococcus Microorganism
to reduce growth and recovery o recovery and growth to reduce Agar MacConkey of Incubation identification. complete for are recommended pure cultures using gram allowing Agar, MacConkey Agar MacConkey Although medium. this on grow to or fail poorly grow that be encountered strains may some variation, nutritional Due to keeping container tightly closed. tightly container keeping - fermenting organisms grow as pink to brick pink grow as organisms fermenting
-
lactose fermenting organisms grow as colorless or clear colonies. Swarming by Swarming orclearcolonies. grow colorless as organisms fermenting lactose
ATCC® 25931 ATCC®
ATCC® 25922 ATCC®
ATCC® 29906 ATCC® 12453 ATCC®
ATCC® 29212 ATCC®
ATCC® 25923 ATCC® the minimum that should be used for quality control testing. qualitycontrol used thatfor beshould minimum the amped on the container. The dehydrated medium should be discarded if not not if discarded be should medium dehydrated The on the container. amped 14028 ATCC® ATCC® 19615 ATCC®
ATCC® 6305 ATCC®
without
without f certain Gram certain f – - positi
30°C away from direct sunlight. Once opened and recapped, place place recapped, and opened Once direct sunlight. from away 30°C
Crystal Violet & Salt is a selective medium, it is less inhibitory than it than isinhibitory less medium, Salt is a &selective Violet Crystal
ve organisms to grow. Biochemical and serological testing testing serological and toBiochemical grow. organisms ve
Crystal Violet & Salt under increased CO increased Salt under & Violet Crystal Inoculum Inoculum Approx. Approx. 50 50 50 50 50 50 (CFU) ~10 >10 >10 ------200 200 200 200 200 200 - negative bacilli. negative - 4 5 5 red colonies with or without a zone of precipitated zone precipitated of a without with or red colonies
Growth Complete Inhibition Complete Inhibition Complete May show partial May show
inhibition Growth
>70% >70% >70% >70% >70% ; suppressed ; suppressed size
Expected Results Expected
2
has been reported reported been has Bright pink colonies Bright pink Colorless colonies, colonies, Colorless colonies, Colorless Colorless Colorless colonies Colorless Colorless to pink pink to Colorless Proteus Proteus Pink colonies Pink swarming +/ swarming no swarming Reaction
colonies -- --
colonies spp. is spp.
-
Revision: 2 Effective Date: 12/16/2019 3. 2. 1. References Sheet Specification Technical
incubation. Abst. Ann. Mtg. American Society for Microbiology. C179. Microbiology. Society for American Mtg. Ann. Abst. incubation. Mazura D.C. Washington, Microbiology, for Society American ed. 6th microbiology, (eds.). Yolken R.H. and C. Tenover, F. Pfaller, M. A. Baron, E. J. R., P. Murray, A. MacConkey, - Reetz, G. T. Neblett, and J. M. Galperin. M. J. Galperin. and Neblett, T. G. Reetz,
1905. Lactose 1905. - fermenting bacteria in feces. J. Hyg. Hyg. J. feces. in bacteria fermenting 1979. MacConkey Agar: CO2 vs. ambient ambient vs. CO2 Agar: MacConkey 1979.
5 :333
- 379. 379.
Manual of clinical of Manual