(12) Patent Application Publication (10) Pub. No.: US 2007/01965.17 A1 San Martin (43) Pub
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US 200701965.17A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2007/01965.17 A1 San Martin (43) Pub. Date: Aug. 23, 2007 (54) MODIFIED SAPONIN MOLLUSCICIDE Related U.S. Application Data (63) Continuation-in-part of application No. PCT/US06/ (75) Inventor: Ricardo Manuel San Martin, Santiago 14458, filed on Apr. 18, 2006. (CL)CL (30) Foreign Application Priority Data Correspondence Address: Jul. 13, 2005 (CL).......................................... 1745-2005 MYERS WOLIN, LLC Publication Classification 100 HEADQUARTERS PLAZA North Tower, 6th Floor (51) Int. Cl. MORRISTOWN, NJ 07960-6834 (US) AOIN 45/00 (2006.01) AOIN 65/00 (2006.01) AOIN 43/04 (2006.01) (73) Assignee: DICTUC S.A., Santiago (CL) (52) U.S. Cl. ............................... 424/725; 514/33; 514/26 (57) ABSTRACT (21) Appl. No.: 11/740,275 Saponins from plant materials are alkaline modified and (22) Filed: Apr. 25, 2007 used as molluscicides. Patent Application Publication Aug. 23, 2007 Sheet 1 of 5 US 2007/01965.17 A1 Ratio husk-water from 1:3-5 Temperature 20-35° C from 0.5 to 1 hr Alkaline treatment NaOH 0.4-0.6N Temperature 90-100° C from 1 to 2 hr pH from 12 to 13 Neutralization pH from 6.5 to 7.5 Hot Air Dry 5-10% w/w moisture content Figure 1: Major processing steps - Powder product Patent Application Publication Aug. 23, 2007 Sheet 2 of 5 US 2007/01965.17 A1 Quinoa husk Extraction Ratio husk-water from 1:8-10 Water Temperature 20-30°C from 0.5-1 h Removal of proteins Bentonite and/or Bentonite 1-5 g/L and/or Acid Acid pH 3.5-4.0 Washing of solids Water Ratio husk-water from 1:5-8 20-30°C from 0.5-1 hr Liquid Solid residue Concentration 200 to 350 g solids/L Alkaline treatment NaOH 0.4-0.6N Temperature 90-100°C from 1.5-2.5 h pH from 12 to 13 Neutralization pH from 6.5 to 7.5 Packaging Figure 2 : Major processing steps - Liquid product Patent Application Publication Aug. 23, 2007 Sheet 3 of 5 US 2007/01965.17 A1 1 kgQuinoa husk Extraction Ratio husk-water 1:3 Temperature 20 °C for 0.5 hr 80 g NaOH + Alkaline treatment 2 L Water Ratio husk-water 1:5 NaOH 0.4-N Temperature 95 °C for 2hr Neutralization Temperature 20 °C pH 7 Air Dry 16 hr at 70 °C Figure 3: Example Processing steps - Powder product Patent Application Publication Aug. 23, 2007 Sheet 4 of 5 US 2007/01965.17 A1 Figure 4b O. F. m t; E. i. -- 3:as - www.xxxx-xx-x-- Figure 4 : RP-HPLC chromatogram of duinoa saponins. Absorbance at 210 nm vs. injection time (min) . Figure 4a: Crude acqueous eXtra Ct, Figure 4b : a CueOuS eXtra Cl purified with ultrafiltration membranes. Peaks A, B and C correspond to main Culin Oa Sap OnlinS. Patent Application Publication Aug. 23, 2007 Sheet 5 of 5 US 2007/01965.17 A1 - is E.. Y - 8. - : st - c n o r, r secrys-----e.is - , ...&rs.s- & k ‘y...sier,t Figure 5: RP-HPLC chromatograms of quinoa husks treated with NaOH. Absorbance at 210 nm vs. injection time (min). US 2007/01965.17 A1 Aug. 23, 2007 MODIFIED SAPONN MOLLUSCCDE industry and with the intent of providing a valuable protein source for farmers, who primarily live on a rice diet. It is 0001. This disclosure is a continuation in part of pending now over 20 years since Golden Apple Snails (Pomacea PCT/US2006/014458 filed Apr. 18, 2006 and claims priority canaliculata) were introduced in Asian countries from its from Chilean application No. 1745-2005 filed Jul. 13, 2005, native habitat in Northern Argentina and Southern Brazil the entire disclosures of which are incorporated by reference with the intention of using them for human consumption. herein. Unfortunately, the imported snails are able to transfer Angiostrongylus cantonensis (rat lungworm) like the native BACKGROUND OF THE INVENTION apple Snail population (Pila). This parasite spends a part of 0002) 1. Field of the Invention its life cycle in apple Snails and can infect humans when the Snails arent cooked thoroughly. 0003. This disclosure relates to the recovery of saponins from plant material. In particular, the invention relates to the 0010 Instead of becoming a food source the snails recovery of saponins from Chenopodium quinoa (Ouinoa) escaped and became a serious threat to rice production and (Chenopodiaceae) husk in commercially useful forms. It the environment. During the 1980s the introduced snails also relates to a method and a composition based on quinoa rapidly spread to Indonesia, Thailand, Cambodia, Hong saponins. The invention also relates to a method for the Kong, Southern China, Japan, the Philippines and Australia. control of fresh water mollusks, in particular, Snails, which In 1989 Pomacea Canaliculata was introduced in Hawaii to comprises the application of a composition based on alkaline serve as a food source and aquarium pet. Some Snails treated quinoa Saponins, in which the dose results in the escaped to the wild and turned into a serious pest in the taro death of the mollusk but not in the death of non-target and rice fields. organisms, such as fish, frogs, etc., thus allowing the safe 0011. As a result of the failure this program, the snails application of the method and product on flooded plantations were released to rivers and rice fields, where they prolifer Such as rice fields, as well as in rivers, ponds, lakes and other ated to Such an extent that they are now considered one of naturally occurring or artificial bodies of water. the 100 worst invasive alien species in the world (ISSG, 0004 The composition comprises liquid and powders 2006). which do not attack beneficial forms of aquatic life when 0012. The Golden Apple Snail, popularly known as used in appropriate amounts. This disclosure also relates to 'golden kuholPomacea canaliculata Lamarck, is one of methods of producing the disclosed compositions. the major pest problems in rice production. In 1989, the 0005 2. Description of Related Art Food and Agriculture Organization of the United Nations estimated that yield losses owing to this pest ranged from I. Snails 1% to 40% of the planted area in the Philippines, resulting 0006 Snails are members of the molluscan class Gas in a huge production loss (The Philippine Rice Research tropoda that have coiled shells. Snails are found in fresh Institute). water, saltwater, and terrestrial environments. Most are 0013 Fresh water snails cause great losses in agriculture herbivorous, though a few land species and many marine and health human problems. Since its introduction in Asia in species may be omnivores or carnivores. The majority of 1985, the golden apple Snail (Pomacea canaliculata) has Snails are not terrestrial. Snails with lungs belong to the caused losses in excess of US $1,000 million in rice crops. group Pulmonata, while those with gills form a paraphyletic Likewise, there are several aquatic snails that transmit group. Schistosomiasis (e.g. Biomphalaria spp.), which is, after 0007. Apple snails are tropical and sub-tropical freshwa malaria, the second most important tropical disease for ter snails of the family Ampullariidae (sometimes referred to humans, affecting more than 200 million people. as Pilidae). They are commonly divided into several genera. 0014. In the water of the rice fields the snails reproduce The genera Asolene, Felipponea, Marisa, and Pomacea are extremely quickly and eat young rice seedlings Voraciously, found in South America, Central America, the West Indies causing significant economical damage to farmers. In addi and the Southern U.S.A., while the genera Afropomus, tion to countries Such as Taiwan, Thailand, Malaysia, Indo Lanistes and Saulea are found in Africa. The genus Pila is nesia and The Philippines which are suffering from exten native in both Africa and Asia. sive damage due to this pest, other countries, such as 0008. Apple snails are the largest living freshwater snails. Australia, consider the pest as a serious potential menace to The Giant Apple Snail, Pomacea maculata (Family Amp its rice agriculture (Plant Health Australia, 2005, pp. 12-13). ullariidae), is the largest freshwater Snail known, with a 0015 The invasion of Pomacea in new habitats has been diameter of up to 15 cm and a mass of over 600 g. Apple shown to drastically alter the state and function of invaded Snails are exceptionally well adapted to tropical regions natural wetlands. When plants are consumed, nutrients in the characterized by periods of drought alternating with periods system are shunted to phytoplankton instead of the plants of high rainfall. One of the more typical adaptations of apple which creates dense algal blooms. Snails is the bronchial respiration system comparable with 0016) Pomacea and Marisa species have been introduced the gills of a fish located at the right side of the snail body in Africa and Asia to control Snails such as Planorbidae, which enables the snails to breathe under water as well as a Bulinus sp. and Biomphalaria sp. which serve as interme lung at the left side of the body to respirate air. diate hosts for trematoda parasites which can cause Swim 0009 Pomacea canaliculata is a common apple snail. In mers itch and schistosomiasis, a disease that affects over 200 the 1980s, snails of the genus Pomacea (Pomacea canali million people in tropical regions. Despite the fact the culata) were introduced in Taiwan to start an escargot tremadote parasites do not complete their life cycle in apple US 2007/01965.17 A1 Aug. 23, 2007 Snails, apple Snails themselves can carry these parasites and turned to commercial molluscicides such as niclosamide and nematodes of the genus Angiostrongylus.